[Histonet] Precision in IHC validations
Thanks to everyone that has responded to my question. We specifically interested in the procedure for this CAP question: COM.40350 Validation of Test Performance Specifications - Modified FDA-cleared/ approved Tests and LDTs Prior to clinical use of each modified FDA-cleared or approved test and laboratory developed tests (LDTs), the laboratory has performed a validation study and prepared a written assessment of each of the following test method performance specifications, as applicable, using a sufficient number of characterized samples: ● Analytical accuracy ● Analytical precision ● Reportable range ● Analytical sensitivity (lower detection limit) ● Analytical specificity ● Any other performance characteristic required to ensure analytical test performance Do you comment at all regarding precision…..do you run the same slide each day over 3 days, or something like that? NOTE 3: Precision is validated by repeat measurement of samples at varying concentrations or activities within-run and between-run over a period of time. We are just not sure what number of repeats is best….. This electronic message is intended only for the use of the individual(s) and entity named as recipients in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from any computer. Do not deliver, distribute or copy this message, and do not disclose its contents or take any action in reliance on the information it contains. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] How are you documenting precision in IHC validations
We are working on IHC antibody validations and I want to know if there is a minimum number of slides you must run to demonstrate precision? I have not been able to find a definitive number or procedure. What is everyone doing for this? Thanks in advance for everyone's help. Martha Ward Atrium Health Wake Forest Baptist This electronic message is intended only for the use of the individual(s) and entity named as recipients in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from any computer. Do not deliver, distribute or copy this message, and do not disclose its contents or take any action in reliance on the information it contains. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Bone marrow clot IHC tissue sections washing
It was brought to my attention that we had significant washing on 3 of 8 bone marrow clot sections the other day; this is not the first time so we would like to get to the bottom of this. We use positively charged slides and all 8 cases were cut and run the same morning but allowed to air dry and then bake at 60C for 20 minutes before being run on our Bond 3 stainer. Has anyone out there experienced this type of problem and if so, what were your solutions? The repeat of the 3 cases today showed similar washing of tissue. This hasn't just started but has occurred periodically but the pathologists have tried to live with it and usually we can finally get enough tissue to stay on after 1-2 attempts. Suggestions include cutting and drying the slides overnight and/or going to a gelatinated slide versus a sialylated slide. We have been using this particular brand of positive charged slide with good results for several years and rarely have issues with other tissue types unless they are particularly bloody. Thoughts or suggestions are greatly appreciated. Martha Ward MT(ASCP) QIHC Atrium Health Wake Forest Baptist ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] antibody protocol for CXCL13
Our heme path docs are interested in this antibody and I wondered if anyone has a protocol they can share?We have the Leica Bond 3 as our staining platform/detection system. Thanks in advance for your help. Martha Ward MT(ASCP) QIHC Manager, Molecular Diagnostics Lab Atrium Health Wake Forest Baptist Health ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] HSV1 and HSV2
Thank you for the information. I will look into your antibody source. Martha -Original Message- From: Cartun, Richard Sent: Tuesday, June 15, 2021 3:26 PM To: Martha Ward-Pathology Cc: histonet@lists.utsouthwestern.edu Subject: [EXTERNAL] RE: HSV1 and HSV2 Hi Martha: We are using type-specific monoclonals from BioSB (Santa Barbara, CA). They also sell a cocktail of the two antibodies which is what we use for screening. Rarely, do we get requests for type-specific identification. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology/Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (Office) (860) 545-2204 (Fax) richard.car...@hhchealth.org -Original Message- From: Martha Ward-Pathology via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Tuesday, June 15, 2021 3:10 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HSV1 and HSV2 CAUTION: This email is from outside HHC. USE CARE when opening attachments or links. We have always offered these two antibodies separately but recently we have begun having issues with our HSV2. In researching a new source I am seeing that some places do some sort of cocktail mixture of the two. What are others out there doing for this? Thanks in advance for your help. Martha Ward, Manager Molecular Diagnostics Lab Wake Forest Baptist Medical Center Winston-Salem, NC 27157 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!KCs9X-8!PM-BBy3bF7epr83ucPrDa0D21BtxagGkNt2mKWWY7SUaExNwbpL43czZRyw5F0A-LMQ$ Reminder: This e-mail and any attachments are subject to the current HHC email retention policies. Please save or store appropriately in accordance with policy. This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] HSV1 and HSV2
We have always offered these two antibodies separately but recently we have begun having issues with our HSV2. In researching a new source I am seeing that some places do some sort of cocktail mixture of the two. What are others out there doing for this? Thanks in advance for your help. Martha Ward, Manager Molecular Diagnostics Lab Wake Forest Baptist Medical Center Winston-Salem, NC 27157 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Filed slides sticking together
I am posting this question for our Histology manager: Does anyone dry coverslipped slides in an oven before filing and if so how long and at what temperature. We are having issues with filed slides sticking together. Thanks in advance for your help with her question. Martha Ward, MT ASCP (QIHC) Manager, Molecular Diagnostics Lab Wake Forest Baptist Medical Center Winston-Salem, NC 27157 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Antibody validation question
When doing an initial antibody validation CAP requires at least 90% concordance between the new test and the comparator test or expected values.Are there specified requirements for sensitivity, specificity and accuracy? Thanks in advance for any help provided! Martha Ward, Manager, Molecular Diagnostics Lab Wake Forest Baptist Medical Center Winston-Salem, NC 27157 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Question about accessioning outside consult cases
We are in the process of switching to AP Beaker and in the midst of the build. My question involves how others are handling outside consult surgical and/or cytology cases. Do you assign them an unique number that identifies them as a consult as opposed to an inside case - SO20- verses S20-? We currently assign them as just another surgical case but I know some places do have outside consult designations. What is everyone doing? Thanks in advance for your input. Martha Ward, MT ASCP (QIHC) Manager, Molecular Diagnostics Lab Wake Forest Baptist Medical Center Winston-Salem, NC 27157 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] "Floaters" in surgical or cytology specimens
I am posting this question for a colleague in our Cytology department. How often do you see floaters on surgical or cytology specimens?Obviously we would never want to see any type of carryover but is there a standard rate published somewhere that he can reference? Thanks in advance for your help. Martha Ward, MT ASCP, QIHC Manager, Molecular Diagnostics Lab Wake Forest Baptist Medical Center ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Tissue processor validation
I am posting this question for my colleague in the Histology lab. She is working on a plan for a new tissue processor validation, including special stains, and would like to get input from anyone that has already gone through it.This is the first time anyone here has ever been involved in validating a new processor. Current ones are 25+ years. Any help or advice would be appreciated. Thanks in advance! Martha Ward, MT (ASCP) QIHC Wake Forest Baptist Health ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] IHC validation after a new tissue processer is installed
Hello all, After a very long time our histology department is getting a new tissue processor, and hopefully several more in the near future. Of course for IHC that means a revalidation/reverification of our IHC stains. Since I have not had to do this before I wanted to get some guidance on how to go about doing this. I feel like I have to completely revalidate the ER, PR and Her2 (20 positive, 20 negative) but wasn't sure about the other antibodies. We have a menu of around 130do I have to test every one of them or can we chose a representative sample? How many antibodies would you suggest? How many positive and negative cases of each should we run? It says it is left up to the medical director but with our CAP inspection next spring we want to make sure we will be fully prepared.What have others done? Thank you in advance for your help with this. Martha Ward, MT (ASCP) QIHC Wake Forest Baptist Medical Center ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Mounting media for immunofluorescence slides
This may be an odd request but I am hoping someone out there can help us with this problem. Our institution is purchasing new digital scanners (Olympus 200) to scan all our pathology case slides. It has come to our attention that the mounting media we have all been using for cover slipping our direct immunofluorescence slides (skins and renal biopsies using FITC IgG, etc. antibodies) is incompatible with the use of these new scanners. We use a hard set mounting media from Vector but it is never quite hard enough to prevent any movement of the cover slip, which is apparently critical to the scanning process and potentially damaging to the scanner.The techs that are using the scanners are advising us to use a permanent mounting media such as Cytoseal 60. Has anyone encountered this problem and was there any downside to using something like Cytoseal 60 on the slides? We plan to test a few control slides to see how they look. Thanks in advance for any advice you can give me. Martha Ward Molecular Diagnostics Lab Wake Forest Baptist Health Winston-Salem, NC 27157 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] PPE for the Histology lab
Our department is looking at safety issues and one suggestion from our CLIA director is that everyone in the lab (our lab performs IHC only) should wear gloves, a gown and eye or face protection at all times and requiring it for anyone that enters the lab. We have task specific PPE requirements for these items to be worn when changing solutions on the stainers or working with serum for our indirect immunofluorescence for example but don't require it for FFPE microtomy or loading the IHC stainers. What are others requiring in their labs? Thanks in advance for your feedback on this issue. Martha Ward Manager, Molecular Diagnostics Lab Wake Forest Baptist Health Winston-Salem, NC 27157 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RUO vs IVD antibodies in immunofluorescence procedures
We are looking for IVD antibodies for C1q and C4d for use on frozen tissues (renal). What vendors are folks using for these direct immunofluorescence stains? Thanks in advance for your help. Martha Ward Wake Forest Baptist Health Winston-Salem, NC 27157 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Looking for vendor for Zeus Wash solution
I am having trouble locating a distributor for the Zeus Wash solution (cat#103) that we use to wash our skin and renal biopsies.Cardinal and Fisher do not seem to carry it and we do not have Zeus Scientific in our system as a vendor we can use. Any help would be appreciated! Thanks, Martha Ward Wake Forest Baptist Health Winston-Salem, NC 27157 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Vendors closed on Presidents Day
Am I the only one that finds it odd and frustrating that some vendors are closed on Presidents Day? I just tried to call the technical service number for Dako about a problem and the message said to call back tomorrow! Hospital labs don't close and I would have thought they would at least have someone staffing the number to cover issues that come up. Thankfully my call isn't urgent but it could have been and then I would be stuck. Martha Ward Wake Forest Baptist Medical Center ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Reproducibility in IHC validations
My medical director and I are working on our procedure for reproducibility in our IHC validations and would like to know how others are handling this. How many slides do you run and over how many days/runs?If someone would share their procedure with us that would be wonderful. Thanks in advance for your help. Martha Ward Wake Forest Baptist Health ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] HPV testing on head and neck tumors and reimbursement
Hello, My billing group wanted me to ask if anyone is seeing denials for reimbursement for HPV testing on male Medicare patients when it is performed on ffpe for head and neck tumors? We have seen a few denials recently and they wanted to see how everyone is handling the coding. This is for CPT code 87624. Thank you in advance for any input you may have on this issue. Martha Ward, MT (ASCP) QIHC Wake Forest Baptist Medical Center Winston-Salem, NC 27157 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Recycling formalin
I am posting this for our Histology lab manager: Is anyone recycling formalin and if so, exactly what are you using the recycled formalin for?Putting it on processors, adding to specimens, etc? Apparently our EHS department is pushing this. Also thank everyone for their help with our questions about unstained slides for prostate biopsies.It was very helpful. Thanks in advance for your help with our recycled formalin question. Martha Ward Wake Forest Baptist Health 336-716-2109 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Prostate needle biopsies
I am posting this question for our Histology manager.For prostate needle biopsies how many levels and unstained slides are people cutting and also how long after the case is signed out is everyone keeping the unstained slides that have been cut? Thanks in advance for everyone's help. Martha Ward Wake Forest Baptist Health Winston-Salem, NC 25157 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Beaker with or without Vantage
"They" aren't the ones that will have to use it.therein lies the rub! I have not heard many positive things about AP Beaker. Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard \ Winston-Salem, NC 27157 p 336.716.2109 \ f 336.716.5890 mw...@wakehealth.edu -Original Message- From: Morken, Timothy via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Tuesday, November 28, 2017 11:48 AM To: Histonet Subject: Re: [Histonet] Beaker with or without Vantage I am interested as well. "They" are threatening us with a move to Beaker in the future Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -Original Message- From: Blake Taylor via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Tuesday, November 28, 2017 8:10 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Beaker with or without Vantage I'm looking for anyone out there that has switched to Beaker for AP, Also do you use Ventana Connect with Beaker? Did you choose to use the Beaker tracking system or is anyone using Beaker in conjunction with Vantage? Our Hospital is in the beginning phase of moving from Copath Sunquest to Beaker (2017 version) . Any thoughts of what has gone well and what has not would be appreciated. Thanks so much Blake Taylor Surgical Pathology Supervisor Lexington Medical Center 803-936-8214 bcdu...@lexhealth.org PRIVILEGED AND CONFIDENTIAL: This electronic message and any attachments are confidential property of the sender. The information is intended only for the use to the person to whom it was addressed. Any other interception, copying, accessing, or disclosure of this message is prohibited. The sender takes no responsibility for any unauthorized reliance on this message. If you have received this message in error, please immediately notify the sender and purge the message you received. Do not forward this message without permission. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Looking for a C4d ffpe control block
Hello everyone, I am trying to find someone who can share a C4d ffpe control block with us. Ours is almost gone and I don't have access to any suitable tissues in house. If anyone has a block they are willing to share please contact me. I can try to help out with any controls that you may be in need of as well. Thanks in advance for everyone's help. Martha Ward, MT (ASCP) QIHC Manager, Molecular Diagnostics Lab Wake Forest Baptist Health Winston-Salem, NC 27157 mw...@wakehealth.edu<mailto:mw...@wakehealth.edu> ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Merkel cell polyomavirus antibody
Good morning, I have had a request for this antibody but I am having trouble locating a vendor for it.If anyone has any information on vendor, etc. , I would appreciate it. We have Leica Bond 3s. Thank you in advance for your help. Martha Ward Wake Forest Baptist Health ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] New IHC instrument validation process
How is everyone handling new instrument validation processes? We are looking at getting a new immunostainer.Beyond the varication process that the installers do what are our responsibilities from a lab stand point? We have a large menu of antibodies.Would it be sufficient to run one of each antibody to demonstrate that the new instrument compares to the old.The detection kits, retrieval solutions, antibodies, etc. would all remain the same. Thanks in advance for your help. Martha Ward Molecular Diagnostics Lab Wake Forest Baptist Health Winston-Salem, NC 27157 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Looking for a maintenance form
Does anyone have a specific form that they use to indicate that a particular instrument is functioning as expected after service repairs or PMs? We need to document this information and I would like a simple form to fill out and include with the service reports.Just trying to not have to reinvent the wheel and would like to see what others are doing. Thanks in advance for your help. Martha Ward Molecular Diagnostics Lab Wake Forest Baptist Health Winston-Salem, NC 27157 336-716-2109 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Dako Herceptest Link kit question
We just installed a new Dako Link Autostainer and have started using the Herceptest Link kit. We had been using two drop zones with our other, older instrument but that was applying only 100 uL in the 2 drop zones. With this new program we set it up to apply two zones of 200 uL but discovered that we will be cutting our test capability in half (50 to about 25 slides). How are other users handling this? Do you use two drop zones, one on each end to cover the control and case material or do you have the reagents apply in one drop zone in the middle of the slide? I am just concerned about the reagents spreading evenly across the slide. What is everyone else's experience with this issue? Thanks in advance for your help! Martha Ward Wake Forest Baptist Health ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] PLA2R assay question
I have been asked to look into offering PLA2R on our renal biopsies and in doing some research on the subject I find that some people are staining frozen tissue via an indirect IMF procedure and some are staining ffpe tissues, also using an indirect IMF procedure.I would prefer to do this via traditional IHC (we have Bond 2 stainers).Is anyone doing this way? If so, could you provide some tips on getting it to work successfully? Thanks in advance for any help. Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard \ Winston-Salem, NC 27157 p 336.716.2109 \ f 336.716.5890 mw...@wakehealth.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Blog Post Not lab related
I agreethe delete button is very easy to use. -Original Message- From: Jennifer MacDonald via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Thursday, April 14, 2016 12:16 PM To: Rene J Buesa Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Blog Post Not lab related In addition to the Histonet, I am on a listserve for clinical laboratory educators. Dr. Raff posts his blog link on that listserve as well. He also responds to some of the questions. There has never been one public complaint regarding his posts. It is very clear in the subject line and the ability to delete his posts are quite easy. Please don't give credence to the myth that the clinical lab has more patience and common sense than the histology world. From: Rene J Buesa via Histonet To: Caroline Miller , Lester Raff MD Cc: "histonet@lists.utsouthwestern.edu" Date: 04/14/2016 06:02 AM Subject:Re: [Histonet] Blog Post Not lab related Amen!!!But you have to concede that Lester is a very persistent, almost obstinate individual, probably used to impose his will and this postings are just an example of it: he likes his blog and tries to impose it to everyone. Evidently he has all the time in the world and just does not know what to do with it and enjoys sharing his "witty" side. René On Wednesday, April 13, 2016 3:24 PM, Caroline Miller via Histonet wrote: Lester, I do believe this is the second 'non lab related' blog post this week. As we have spoken about before I do not think histonet is an appropriate place for your blog posts. I, personally, was totally OK with you co-posting with other lab related topics. However you are now pushing it and I respectfully ask that you refrain from sending out your blog posts to the whole list, let people sign up if they are interested. The issue is that this is a histology related list, if everyone posted non-lab stuff here it would soon be chaos! Respectfully yours, mills On Wed, Apr 13, 2016 at 11:38 AM, Lester Raff MD via Histonet < histonet@lists.utsouthwestern.edu> wrote: > Just some philosophy towards the end of a long day. > > > http://www.chicagonow.com/downsize-maybe/2016/04/kitten-power-can-get-things-done/ > > > > Just a reminder-I try to limit my blog invitations here. If you enjoy the > blog, remember to subscribe (no charge, no spam) on the ChicagoNow blog > site. > > Thanks for your readership. > > Lester J. Raff, MD MBA > UroPartners > Medical Director Of Laboratory > 2225 Enterprise Dr. Suite 2511 > Westchester, Il 60154 > Tel: 708-486-0076 > Fax: 708-492-0203 > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Caroline Miller (mills) Director of Histology 3Scan.com 415 2187297 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Billing
Yes that is how we do it as well. Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard \ Winston-Salem, NC 27157 p 336.716.2109 \ f 336.716.5890 mw...@wakehealth.edu -Original Message- From: Vickroy, James [mailto:jvick...@springfieldclinic.com] Sent: Friday, May 29, 2015 3:13 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Billing Just need a reminder: If a pathologist orders the same several IHC stains on two blocks from the same specimen am I correct to think that we can only charge the stains on one of the blocks? 88342 and the remainder 88341's Jim Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois 62703 Office: 217-528-7541, Ext. 15121 Email: jvick...@springfieldclinic.com<mailto:jvick...@springfieldclinic.com> This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] IHC billing question
That's what we do as well. Martha Ward Wake Forest Baptist Health From: Weems, Joyce K. [joyce.we...@emoryhealthcare.org] Sent: Friday, May 01, 2015 9:13 AM To: 'Cartun, Richard'; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] IHC billing question I do it every day - change every first to 88342. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.we...@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cartun, Richard Sent: Thursday, April 30, 2015 5:33 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC billing question Effective January 1, 2015, our LIS team removed all of the CPT 88342 codes for IHC from our CoPath stain dictionary since you couldn't tell whether a Cytokeratin-7 was being performed as an "88341" or as an "88342". Now, as you might have expected, none of the "inpatient" IHC testing has been accounted for (the outpatient IHC has been billed manually from the pathology report), and they want someone to go back and enter all the CPT codes into the system (hopefully, not me!). Has anyone else encountered this problem? Thanks (I think). Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] IHC billing question
We do our billing manually through CoPath as well. From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of Mike Pence [mpe...@grhs.net] Sent: Thursday, April 30, 2015 5:44 PM To: 'WILLIAM DESALVO'; Cartun, Richard Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] IHC billing question We do all of our IHC billing manually. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of WILLIAM DESALVO Sent: Thursday, April 30, 2015 4:43 PM To: Cartun, Richard Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] IHC billing question We have to manually review the IHC billing also and continue to audit. It took billing and IT three months to create the logic to automate billing for a specimen and account for combination of there being the possibility of 88341, 88342 & 88344 and the proper combinations. Sent from my iPhone > On Apr 30, 2015, at 2:34 PM, Cartun, Richard > wrote: > > Effective January 1, 2015, our LIS team removed all of the CPT 88342 codes > for IHC from our CoPath stain dictionary since you couldn't tell whether a > Cytokeratin-7 was being performed as an "88341" or as an "88342". Now, as > you might have expected, none of the "inpatient" IHC testing has been > accounted for (the outpatient IHC has been billed manually from the pathology > report), and they want someone to go back and enter all the CPT codes into > the system (hopefully, not me!). Has anyone else encountered this problem? > Thanks (I think). > > Richard > > Richard W. Cartun, MS, PhD > Director, Histology & Immunopathology > Director, Biospecimen Collection Programs Assistant Director, Anatomic > Pathology Hartford Hospital > 80 Seymour Street > Hartford, CT 06102 > (860) 972-1596 > (860) 545-2204 Fax > > > This e-mail message, including any attachments, is for the sole use of the > intended recipient(s) and may contain confidential and privileged > information. Any unauthorized review, use, disclosure, or distribution is > prohibited. If you are not the intended recipient, or an employee or agent > responsible for delivering the message to the intended recipient, please > contact the sender by reply e-mail and destroy all copies of the original > message, including any attachments. > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: IHC Billing Question
We have not been charging for the negative control, assuming that it was just a cost of doing business. I would be interested to hear if anyone has been charging for their negative controls as well. Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard \ Winston-Salem, NC 27157 p 336.716.2109 \ f 336.716.5890 mw...@wakehealth.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Burnett, Brandy Sent: Thursday, April 16, 2015 9:48 AM To: 'Joana Moreira'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: IHC Billing Question We recently added HER2 IHC testing in our lab which we are required to use a negative reagent control For each case. Is there a cpt code for negative reagent control reimbursement? Any information on this Would be much appreciated! Thanks Brandy Burnett Histotechnoligist, QIHC(ASCP) CCH Pathology/Histology Expert physicians. Quality hospitals. Superior care. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Joana Moreira Sent: Thursday, April 16, 2015 7:25 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC Billing Question Greetings from Doha! This was much probably discussed before, but I was wondering if you could help me with a query in regards to billing. For the sites that are still doing an IHC negative reagent control for each patient specimen, do you bill for the negative control? Using code 88341? I believe it should be billed (since when and if performed correctly the negative control follows a normal IHC technique) however I am completely new to the billing subject. My previous experience is based in Portugal and UK (where billing does not exist) and I've been introduced to this topic since I joined my current institution that will be following the North American Healthcare model. So... any help will be GREATLY appreciated!! Many Thanks in advance, Joana Joana Moreira Supervisor - Anatomical Pathology Department of Pathology Sidra Medical & Research Center PO Box 26999 | Doha, Qatar Direct Line +974-4404-2036 jmore...@sidra.org | www.sidra.org Disclaimer: This email and its attachments may be confidential and are intended solely for the use of the individual to whom it is addressed. If you are not the intended recipient, any reading, printing, storage, disclosure, copying or any other action taken in respect of this e-mail is prohibited and may be unlawful. If you are not the intended recipient, please notify the sender immediately by using the reply function and then permanently delete what you have received. Any views or opinions expressed are solely those of the author and do not necessarily represent those of Sidra Medical and Research Center. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ~~ This email and any files transmitted with it are confidential, and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error contact the Help Desk for Cape Cod Healthcare. helpd...@capecodhealth.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Need paraffin control block for C4d on kidney tissue
Our current kidney paraffin tissue control that we use for C4d by IHC, as well as the full Ig panel on ffpe kidneys, is getting thin and we are having some difficulty finding a suitable control. Does anyone has a block they would be willing to send us? I may be able to trade for another control block. Thanks in advance for your help. Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard \ Winston-Salem, NC 27157 p 336.716.2109 \ f 336.716.5890 mw...@wakehealth.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Her2 IHC validation
In advance of preparing for our CAP inspection window, I am working on our validation write-up for our Her2 IHC and was looking for some data concerning the correlation rates.We compared our IHC staining to FISH Her2 results on the same case. Is there a minimum correlation rate? I have been unable to find one in my reading. Thank you in advance for your help. Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard \ Winston-Salem, NC 27157 p 336.716.2109 \ f 336.716.5890 mw...@wakehealth.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] p16 Antibody
We dilute ours 1:5 from the predilute and run it on the Bond 3. It works very well. Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard \ Winston-Salem, NC 27157 p 336.716.2109 \ f 336.716.5890 mw...@wakehealth.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Burnett, Brandy Sent: Wednesday, January 14, 2015 10:15 AM To: 'sarah.dys...@stdavids.com'; lseb...@uwhealth.org; veronique.bar...@gmail.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] p16 Antibody We use the Ventana (INK4a) clone as well. What dilution do you use? Brandy Burnett Histotechnoligist, QIHC(ASCP) CCH Pathology/Histology 508-862-5267 bburn...@capecodhealth.org Expert physicians. Quality hospitals. Superior care. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of sarah.dys...@stdavids.com Sent: Wednesday, January 14, 2015 10:01 AM To: lseb...@uwhealth.org; veronique.bar...@gmail.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] p16 Antibody Ventana unfortunately has the market on the "good" clone...you can dilute it out pretty far (it comes pre-diluted) and still get good results however... Sarah Dysart BA, HT (ASCP), QIHC (ASCP) Pathology Supervisor North Austin Medical Center 12221 North Mopac Expressway Austin, TX 78758 512-901-1220 (lab) 512-901-6659 (office) -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sebree Linda A Sent: Wednesday, January 14, 2015 8:41 AM To: 'Véronique Barrès'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] p16 Antibody We use one from BD, Veronique, with good results. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory, Rm A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Véronique Barrès Sent: Wednesday, January 14, 2015 8:06 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] p16 Antibody Hi Histonetters! I am working in a research lab and we sometimes need to do p16 staining, but our pathologist told us that the antibody we are using right now is not good. I'd like to know which antibody you're using in your labs? Thanks for your help and happy Wednesday! Véronique ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ~~ This email and any files transmitted with it are confidential, and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error contact the Help Desk for Cape Cod Healthcare. helpd...@capecodhealth.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: EDTA decalcification solution follow up questions
Thank you to everyone who has responded. They have a more specific question: Currently they are using SurgiPath DeCal solution II. They are being asked to use an EDTA decal solution that will provide the best preservation of RNA and DNA proteins. What is everyone using for this purpose? Thanks again for any information Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard \ Winston-Salem, NC 27157 p 336.716.2109 \ f 336.716.5890 mw...@wakehealth.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Martha Ward-Pathology Sent: Monday, December 01, 2014 2:21 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] EDTA decalcification solution I am asking this question for our Histology Lab. They are being asked about using EDTA as a decal solution for bone and wondered if anyone else is using this?Is this available as a ready to use? Or do you have to make it up? What vendors are you using and could you provide a procedure about how you use itlength of time in solution, etc. Thanks in advance for your help. Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard \ Winston-Salem, NC 27157 p 336.716.2109 \ f 336.716.5890 mw...@wakehealth.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: back orders from various vendors
At least I know I am not crazy or being paranoid! :) this year just seems to be worse than I ever remember it beingand the worse part is while I am freaking out because I can't get something I desperately need, the customer service person can only say "We are sorry for the inconvenience". Argg -Original Message- From: Marcum, Pamela A [mailto:pamar...@uams.edu] Sent: Tuesday, December 02, 2014 10:59 AM To: Martha Ward-Pathology; histonet@lists.utsouthwestern.edu Subject: RE: back orders from various vendors Happens every year at this time with various vendors and end of year. I always over order in October to be sure I am good through the end of the year or as close as I can. I know the things I use most and those are just what is always in backorder at the end of the year. October became my answer as long as expiration dates are not an issue. Pam Marcum UAMS -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Martha Ward-Pathology Sent: Tuesday, December 02, 2014 9:48 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] back orders from various vendors I just need a moment to ventis it just me or is everyone seeing more and more back order issues with their various vendors? It is not specific to any one particular vendor, product or even category of product, but lately I am being told more and more often that an item is not availableexpected release dates anywhere from a few days to 6-8 weeks! This is starting to cause real problems . Thanks for letting get this off my chest.I will resume my frantic search for another antibody vendor now..... Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard \ Winston-Salem, NC 27157 p 336.716.2109 \ f 336.716.5890 mw...@wakehealth.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] back orders from various vendors
I just need a moment to ventis it just me or is everyone seeing more and more back order issues with their various vendors? It is not specific to any one particular vendor, product or even category of product, but lately I am being told more and more often that an item is not availableexpected release dates anywhere from a few days to 6-8 weeks! This is starting to cause real problems . Thanks for letting get this off my chest.I will resume my frantic search for another antibody vendor now. Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard \ Winston-Salem, NC 27157 p 336.716.2109 \ f 336.716.5890 mw...@wakehealth.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] EDTA decalcification solution
I am asking this question for our Histology Lab. They are being asked about using EDTA as a decal solution for bone and wondered if anyone else is using this?Is this available as a ready to use? Or do you have to make it up? What vendors are you using and could you provide a procedure about how you use itlength of time in solution, etc. Thanks in advance for your help. Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard \ Winston-Salem, NC 27157 p 336.716.2109 \ f 336.716.5890 mw...@wakehealth.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] RE: New CPT Codes for IHC
We do our billing as Joyce does as well. Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard \ Winston-Salem, NC 27157 p 336.716.2109 \ f 336.716.5890 mw...@wakehealth.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Miller, Suzie Sent: Wednesday, October 08, 2014 4:34 PM To: Blazek, Linda; Horn, Hazel V; 'Weems, Joyce K.'; 'Andrew Horvath'; 'Adesupo, Adesuyi (Banjo)'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: New CPT Codes for IHC We also interpret the coding to be as stated below by Joyce. From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of Blazek, Linda [lbla...@digestivespecialists.com] Sent: Wednesday, October 08, 2014 3:51 PM To: Horn, Hazel V; 'Weems, Joyce K.'; 'Andrew Horvath'; 'Adesupo, Adesuyi (Banjo)'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: New CPT Codes for IHC Joyce -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V Sent: Wednesday, October 08, 2014 3:45 PM To: 'Weems, Joyce K.'; 'Andrew Horvath'; 'Adesupo, Adesuyi (Banjo)'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: New CPT Codes for IHC I would like the correct answer for this. I have always billed as Joyce states. But a sister hospital says it's like Andrew's email. Who is right? Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hor...@archildrens.org archildrens.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K. Sent: Monday, October 06, 2014 3:41 PM To: 'Andrew Horvath'; 'Adesupo, Adesuyi (Banjo)'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: New CPT Codes for IHC Sorry, I left that part out. We don't do them, so I just didn't think. I understand the 88343 codes to be for multiple stains on the same slide. The first is 88342 additional 88343 - e.g. PIN4. So has that changed? Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.we...@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -Original Message- From: Andrew Horvath [mailto:ahorv...@cogipath.com] Sent: Monday, October 06, 2014 3:36 PM To: Weems, Joyce K.; 'Adesupo, Adesuyi (Banjo)'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: New CPT Codes for IHC Actually, for non-Medicare if there are multiple stains performed on a block, where the initial CPT is 88342, any subsequent billing for stains on that block would actually be 88343 from the information I see. Please refer to the links below for more information... http://ahsrcm.com/assets/2014-Pathology-CPT-Code-Changes.pdf https://www.aapc.com/memberarea/forums/showthread.php?t=101402 quote from the forum: Quite and uproar about this in our office - and a lot of confusion. Go to page 360 in this document to read Medicare's decision about this. (inactive link removed) "The CPT Editorial Panel revised the existing immunohistochemistry code, CPT code 88342 and created a new add-on code 88343 for CY 2014. Current coding requirements only allow CPT code 88342 to be billed once per specimen for each antibody, but the revised CPT codes and descriptors would allow the reporting of multiple units for each slide and each block per antibody (88342 for the first antibody and 88343 for subsequent antibodies). We believe that this coding would encourage overutilization by allowing multiple blocks and slides to be billed. To avoid this incentive, we are creating G0461 (Immunohistochemistry or immunocytochemistry, per specimen; first single or multiplex antibody stain) and G0462 (Immunohistochemistry or immunocytochemistry, per specimen; each additional single or multiplex antibody stain (List separately in addition to code for primary procedure)) to ensure that the services are only reported once for each antibody per specimen. We believe this will result in a
RE: [Histonet] Kim's question - order documentation
I think this is an interesting question. We frequently get phone calls from clinicians asking for ER, PR, Her2 or sometimes just other IHC stainsjust yesterday someone wanted CYK 7 and CYK 20 on a cytology block. We ask that they either call the pathologist who signed out the case and get them to order the stains, or with something like the breast panel, ask that they fax or email us, stating exactly what they want, the patient demographics and surgical number, etc. That way at least we have a paper trail for the files should anyone ask why we did the testing. Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard \ Winston-Salem, NC 27157 p 336.716.2109 \ f 336.716.5890 mw...@wakehealth.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cheryl Sent: Tuesday, October 07, 2014 1:14 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Kim's question - order documentation Kim- Your histonet question may not be as complicated as it might seem. Sometimes it's easier to look at these things backwards. What is the desired outcome? If there is an order -- say for a GMS--and it wasn't ordered by one of your pathologists, where did it come from? Can you track back and figure out what doctor ordered it and verify it's a valid request so the testing AND billing is appropriate (not fraudulent). When the surgeon or clinician collects the sample at the surgery or in their office, sometimes they want something specific -- say 'evaluate for fungus'. They may include this in the surgical notes, the office chart -- other places. His support staff will copy this onto the requisition or somewhere you get the request other than the requisition. If you keep copies of the req and other incoming documentation-- you've satisfied the requirement--you can track the source of the order. If you don't, include it in the gross description or notes that are transcribed onto the report so that you have a durable record that you can find (may take a while if it's the archived chart, but you can find it). This goes back to the requirement that orders can't just come from anyone or for any wild-hair reason-- and you have to be able to substantiate or prove the valid source of an ordered (and billed) test. Does that help? Cheryl Cheryl Kerry, HT(ASCP) Full Staff Inc. Staffing the AP Lab - one Great Tech at a time. 281.852.9457 Office 800.756.3309 Phone and Fax ad...@fullstaff.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: questions
Sounds like we are all working at the same place! LOL I hear exactly the same questions.is it something they teach to all pathology residents Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard \ Winston-Salem, NC 27157 p 336.716.2109 \ f 336.716.5890 mw...@wakehealth.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Thursday, August 07, 2014 1:18 PM To: 'Grantham, Andrea L - (algranth)'; Webb, Dorothy L; 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] RE: questions "... and I can still hear myself explaining to them that there could only be ONE first!" Yes, DAILY!!! Every time someone wants to change things around so they get theirs first, this is our only defense as to why everyone can't get theirs first. 8 years of college doesn't seem to help here... Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Grantham, Andrea L - (algranth) Sent: Thursday, August 07, 2014 10:08 AM To: Webb, Dorothy L; 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] RE: questions When I worked in clinical labs the order was first the priority cases (biopsies, bone marrows, etc.) then numerically. I remember once two physicians brought small biopsies and were in a hurry for the results, both wanted to be first out and I can still hear myself explaining to them that there could only be ONE first! Andi From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of Webb, Dorothy L [dorothy.l.w...@healthpartners.com] Sent: Thursday, August 07, 2014 9:54 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] questions I have a couple of questions to ask where there is no right or wrong answer, just curious as to the process that other labs use. 1. After processing, how do you determine the order in which to cut and stain the blocks..numerical or priority driven? 2. Do you adhere to the 6-72 hours of fixation for breasts or make certain all breast tissue is fixed for a minimum time of, say, 24 hours but, of course no longer than 72? I appreciate your responses and thanks for your time!! I am retiring at the end of this year and trying to optimize some processes beforehand:). Dorothy Webb, HT (ASCP) Regions Histology Technical Specialist 651-254-2962 This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this communication in error, please return it to the sender immediately and delete the original message and any copy of it from your computer system. If you have any questions concerning this message, please contact the sender. Disclaimer R001.0 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: rolling sections
We do the same thing on our lab. It isn't necessary for them to rollwe just catch them and fold them up and put them in the tube. Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard \ Winston-Salem, NC 27157 p 336.716.2109 \ f 336.716.5890 mw...@wakehealth.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Helen Fedor Sent: Thursday, June 12, 2014 10:55 AM To: 'Roberta Horner'; Histonet (histonet@lists.utsouthwestern.edu) Subject: [Histonet] RE: rolling sections Hi, I think that it is not necessary to actually get them to roll. We just collect all of the sections and put them into the tube. Scrunched, not rolled. Helen -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Roberta Horner Sent: Thursday, June 12, 2014 10:42 AM To: Histonet (histonet@lists.utsouthwestern.edu) Subject: [Histonet] rolling sections I have some researchers that want to do PCR. They want 10 - 10u sections in a micro-centrifuge tube. The only way to get the sections in the tube is for the sections to roll. How do you get sections to roll when you want them to roll? I've tried room temperature, on ice, brand new sharp blade, dull blade and I can still get some really nice ribbons. When I want a thick ribbon it will roll, darn that Murphy and his laws. Roberta Horner Animal Diagnostic Lab Penn State University ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Re: Negative Controls
So do we. Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard \ Winston-Salem, NC 27157 p 336.716.2109 \ f 336.716.5890 mw...@wakehealth.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sebree Linda A Sent: Wednesday, April 30, 2014 8:35 AM To: 'Campbell, Tasha M.'; Terri Braud; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Negative Controls Tasha, We use the negative elements within our patient sample as our "negative tissue control". Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Campbell, Tasha M. Sent: Wednesday, April 30, 2014 6:48 AM To: Terri Braud; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Negative Controls So this has confused me more. So before you would run a negative control for each block you were testing and you would use the negative mouse or rabbit reagent. Now you don't have to do that but you still need a negative tissue control. So what exactly does this mean? Does it mean for every antibody that you are running you need to have a negative tissue control for it? So instead of using the negative mouse serum you would run a known negative tissue control with the antibody, say CD3 or whatever it is? So are most people doing a control slide with a negative tissue and a positive tissue on it? Tasha Campbell, B.S.,HTL(ASCP) Frederick Gastroenterology Associates 310 W. 9th St. Frederick, MD 21701 301-695-6800 ext. 144 (w) 304-685-9307 (c) -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Terri Braud Sent: Tuesday, April 29, 2014 1:30 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Negative Controls On Message 7 - Negative Controls While it is true that if you run polymers, you no longer have to run a negative reagent control, HOWEVER, you still must have a negative tissue control, which to quote CAP: "must show no staining of tissues known to lack the antigen" Any of the following can serve as a negative tissue control: 1. Multi tissue blocks. These can provide simultaneous positive and negative tissue controls and are considered "best practice"... The type of negative tissue control used (i.e. separate sections, internal controls, or multitissue blocks) must be specified in the laboratory manual." Thus sayeth CAP, the almighty. Please see ANP.22570 Our lab has defined our negative controls as a piece of Uterus as the negative tissue in a multitissue block as a negative tissue control for most of our antibodies, though for a few that might be too reactive in uterus, we use a piece of skin. I hope this helps. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Holy Redeemer Hospital Laboratory 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3676 Fax: 215-938-3874 Message: 7 From: Beth Brinegar Subject: [Histonet] Negative controls Hello fellow histonetters, What is are other labs doing to satisfy the ANP.22570 QC - Antibodies "Appropriate negative controls are used." - CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] CAP question ANP.22978 - Her2 assay validation
Hello all, I have been reading through the most recent revisions and want to see how others are handling this question. The explanation states that it is for new and existing assays and that if your validation does not meet current standards that you must supplement and bring it into compliance. Furthermore if you do not have any documentation from the initial validation the assay must be fully revalidated and documented. Our lab has been performing the Herceptest from Dako (FDA approved) since before 2008 and participating in the HER2 proficiency testing since it was first offered. We have our statistical results comparing our IHC patient results to FISH Her2 results since 2008 and we have always done well on our CAP proficiency testing (95%-100%).We do inter-pathologist result comparisons, using know CAP slides and have 95% to 100% agreements. What I do not have however is the original results of the slides that were stained to set up the original assay. Under these circumstances will we need to completely revalidate the assay, using the mandated 20+/20- cases, or can we simply do a retroactive formal review and write up of our past performances on our proficiency testing challenges? Thanks in advance for your help with this! Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard \ Winston-Salem, NC 27157 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Instrument Verification
I'm with you. There really appears to be no value to this particular requirement.I would only be concerned with it if I had just purchased it, or moved it into our lab from another location. Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard \ Winston-Salem, NC 27157 p 336.716.2109 \ f 336.716.5890 mw...@wakehealth.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cynthia Robinson Sent: Thursday, April 03, 2014 3:03 PM To: Terri Braud; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Instrument Verification I agree with you in that CAP is just looking for things to change and doesn't seem to be considering the change and decrease in staffing seen in clinical settings. Cryostat validation? Reallycut a slide after you have cleaned and pm'd the thing and go on. Good grief...I don't need any more paper and documentation on routine processes. As for tissue processors, I have 20 year old VIP's that have been running and producing specimens acceptably. I did validate them prior to being put in use but we didn't document like we do now. And I don't see the need to do it at this stage of use. We did do a very extensive validation on the Peloris we put into use last year and will going forward on new equipment. To me the daily QC of stain should provide our 'validation' of the process and include the processor. I am interested in others thoughts as well. Thanks for allowing me to rant. Cindi Robinson, HT(ASCP) Mercy Medical Center-Sioux City Dunes Medical Laboratories 350 W Anchor Drive Dakota Dunes SD 57049 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Terri Braud Sent: Thursday, April 03, 2014 1:00 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Instrument Verification I just received my midcycle CAP and for cryostat validation, we are planning to cut and stain a piece of frozen tonsil and have the path sign off on it. For the tissue processors, we will run a one minute test program. I hope this will fly. Is it just me, or is CAP insanely out of control with new or modified regulations and policies for AP? Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Holy Redeemer Hospital Laboratory 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3676 Fax: 215-938-3874 6. Validation of cryostat (Gloria Tharp) Message: 6 Date: Thu, 3 Apr 2014 09:59:26 -0500 From: "Gloria Tharp" Could anyone tell me how you are handling the new CAP ANP.23045 question on function and verification of equipment regarding a cryostat. Gloria Tharp, BA, HTL(ASCP) -- Message: 7 Date: Thu, 3 Apr 2014 15:26:17 + From: "Leann M. Murphy" How is everyone validating the tissue processor for new CAP ANP.23045 question on function and verification of equipment? LeAnn Murphy Aultman Hospital Canton, Ohio - CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] RE: Negative Controls for IHC
We discontinued them over a year ago. Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard \ Winston-Salem, NC 27157 p 336.716.2109 \ f 336.716.5890 mw...@wakehealth.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of joelle weaver Sent: Wednesday, February 12, 2014 11:04 AM To: Cheri Miller Cc: histonet@lists.utsouthwestern.edu; histonet-boun...@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Negative Controls for IHC Cheryl Yes, I understand. It is highly likely this will be the way things go here also. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: cmil...@physlab.com > To: joellewea...@hotmail.com > CC: histonet-boun...@lists.utsouthwestern.edu; > histonet@lists.utsouthwestern.edu > Date: Wed, 12 Feb 2014 08:04:43 -0600 > Subject: RE: [Histonet] RE: Negative Controls for IHC > > I just stopped using the reagent controls on all our IHC due to the drastic > cuts in reimbursement. It will be a significant immediate savings for our > tiny lab. > Cheryl A. Miller HT ASCP cm > Histology Supervisor > Hygiene Officer > Physicians Laboratory, P.C. > 4840 F St. > Omaha , NE. 68117 > 402 731 4145 ext. 532 > Cell 402 493 0403 > > From: histonet-boun...@lists.utsouthwestern.edu > [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of joelle weaver > [joellewea...@hotmail.com] > Sent: Tuesday, February 11, 2014 1:54 PM > To: Sebree Linda A; 'Abbott, Tanya'; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] RE: Negative Controls for IHC > > Not too long ago, there was a posting about labs that had discontinued. You > technically don't need with a polymer, non-biotin system. I still do them- > for now. The pathologist never uses them, internal controls only. But I do, > but this may be a good cost cutting idea for the future. > > > > > Joelle Weaver MAOM, HTL (ASCP) QIHC > > > From: lseb...@uwhealth.org > > To: tanyaabb...@catholichealth.net; > > histonet@lists.utsouthwestern.edu > > Date: Tue, 11 Feb 2014 19:44:37 + > > CC: > > Subject: [Histonet] RE: Negative Controls for IHC > > > > Hi Tanya, > > > > We have made the decision to continue running negative reagent control > > slides. > > > > Linda A. Sebree > > University of Wisconsin Hospital & Clinics IHC/ISH Laboratory > > 600 Highland Ave. > > Madison, WI 53792 > > (608)265-6596 > > FAX: (608)262-7174 > > > > -Original Message- > > From: histonet-boun...@lists.utsouthwestern.edu > > [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of > > Abbott, Tanya > > Sent: Tuesday, February 11, 2014 12:54 PM > > To: histonet@lists.utsouthwestern.edu > > Subject: [Histonet] Negative Controls for IHC > > > > I just read in Advance Dec 2013 that there is a possibility of laboratories > > utilizing fewer QC controls to cut costs? Has anyone stopped running > > negative controls for IHC? > > > > Tanya G. Abbott RT (CSMLS) > > Manager Technologist, Histology/Cytology St. Joseph Medical Center > > Reading, PA 19603-0316 ph 610-378-2635 fax 610-898-5871 > > email: tanyaabb...@catholichealth.net > > > > This electronic mail and any attached documents are intended solely for the > > named addressee(s) and contain confidential information. If you are not an > > addressee, or responsible for delivering this email to an addressee, you > > have received this email in error and are notified that reading, copying, > > or disclosing this email is prohibited. If you received this email in > > error, immediately reply to the sender and delete the message completely > > from your computer system. > > ___ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ___ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If > you are not the addressee intended / indicated or agent responsible for > delivering it to the addressee, you are hereby notified that
RE: [Histonet] Turn Around Time
Rene, No not the quality of the slides at all. Actually my lab does not do the processing and initial H&E, which is done in our main histology lab. My concern is that the push for quicker TATs sometimes leads to inadequate fixation, etc., which affects our lab.I'm just saying that fast does not always with better, but there is no reason not to stream line our processes and try to provide more timely results. Of course any case requiring our lab services (IHC, ISH, PCR) is going to have a longer TAT. We generally have a <24 hour TAT on our IHC and ISH and of course longer on our PCR testing as those are batched. More of an editorial comment that anything else Martha From: Rene J Buesa [mailto:rjbu...@yahoo.com] Sent: Monday, February 03, 2014 4:38 PM To: Martha Ward-Pathology; Dawn Bugge; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Turn Around Time Martha: So, do you mean that you are not satisfied with the quality of your slides? If you are, I just do not understand your concerns about a 24 h TAT! The fundamental issue is to develop adequate protocols assuring quality as well as a convenient (24 h) TAT. Just my 3 cents (after inflation!) René J. From: Martha Ward-Pathology mailto:mw...@wakehealth.edu>> To: Dawn Bugge mailto:drbu...@gmail.com>>; "histonet@lists.utsouthwestern.edu<mailto:histonet@lists.utsouthwestern.edu>" mailto:histonet@lists.utsouthwestern.edu>> Sent: Monday, February 3, 2014 2:05 PM Subject: RE: [Histonet] Turn Around Time While I recognize the need for a quick result in some cases, I also subscribe to the theory that "speed kills". I'm not sure that these quick TATs are always medically necessary, but rather more of a convenience.However that is another discussion altogether. That said, our institution shoots for the 80% in 24 hours as well. Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard \ Winston-Salem, NC 27157 p 336.716.2109 \ f 336.716.5890 mw...@wakehealth.edu<mailto:mw...@wakehealth.edu> -Original Message- From: histonet-boun...@lists.utsouthwestern.edu<mailto:histonet-boun...@lists.utsouthwestern.edu> [mailto:histonet-boun...@lists.utsouthwestern.edu<mailto:histonet-boun...@lists.utsouthwestern.edu>] On Behalf Of Dawn Bugge Sent: Monday, February 03, 2014 12:43 PM To: histonet@lists.utsouthwestern.edu<mailto:histonet@lists.utsouthwestern.edu> Subject: [Histonet] Turn Around Time Hello Histonet I am just curious what the standard for Turn Around Time is for most labs. I think a two day turn around time from the time the biopsy gets to the lab to the time the pathologist signs out a case is pretty fast. Thanks for your input. -- Dawn R Bugge Seattle Histology ___ Histonet mailing list Histonet@lists.utsouthwestern.edu<mailto:Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu<mailto:Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Turn Around Time
While I recognize the need for a quick result in some cases, I also subscribe to the theory that "speed kills". I'm not sure that these quick TATs are always medically necessary, but rather more of a convenience.However that is another discussion altogether. That said, our institution shoots for the 80% in 24 hours as well. Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard \ Winston-Salem, NC 27157 p 336.716.2109 \ f 336.716.5890 mw...@wakehealth.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Dawn Bugge Sent: Monday, February 03, 2014 12:43 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Turn Around Time Hello Histonet I am just curious what the standard for Turn Around Time is for most labs. I think a two day turn around time from the time the biopsy gets to the lab to the time the pathologist signs out a case is pretty fast. Thanks for your input. -- Dawn R Bugge Seattle Histology ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: HER2/NEU
That is an interesting observation, as we have seen something of the same thing and we use the Dako Herceptest.I would be interested in hearing others responses as well. Thank you for asking the question. Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard \ Winston-Salem, NC 27157 p 336.716.2109 \ f 336.716.5890 mw...@wakehealth.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Knutson, Deanne Sent: Wednesday, January 15, 2014 11:46 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] HER2/NEU I just wanted to see what others in the histology world might be experiencing. We have seen a marked increase in the percentage of HER2 cases resulting as Indeterminate, when we compare our last year patient predictive marker results to this year's results. The clone we are using is CB11 from Cell Marque. We did a HER2 study last year where we sent out our cases for a second opinion, and the study showed others interpreted our Indeterminates as that also. Has anyone else also noted this increase in their annual comparison, and what clone are you using? It is so nice to be able to reach out to our peers with these types of questions. Thank you for responding back! Deanne Knutson Anatomic Pathology Supervisor St. Alexius Medical Center 701-530-6730 dknut...@primecare.org<mailto:dknut...@primecare.org> This email may include confidential and privileged information. If this is not intended for your use, please destroy immediately and contact the sender of the message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Vendor suggestions for IgG subclass antibodies
I am looking for a vendor for FITC labeled IgG1, IgG2 IgG3 and IgG4 antibodies for use in our immunofluorescence panels when staining renal biopsies. We get most of our FITC antibodies for our panel from Dako but I didn't see these others. Any suggestions are appreciated. Thanks in advance for your help! Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard \ Winston-Salem, NC 27157 p 336.716.2109 \ f 336.716.5890 mw...@wakehealth.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] IgG sub classes and anti-PLA2R in renals
I am looking for information on IgG sub class antibodies, as well as anti-PLA2R, for use in renal biopsies. Specifically using immunofluorescence. Is anyone using these antibodies routinely in their renal biopsy protocols? If so, would you mind sending me information such as vendors, etc.Actually any information is appreciated.I have just had a inquiry from our renal pathologist about offering these. Thanks in advance for your help. Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard \ Winston-Salem, NC 27157 p 336.716.2109 \ f 336.716.5890 mw...@wakehealth.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: cell block staining issues
Well actually it does help because we are trying to eliminate possible causes and it is nice to know that someone else uses Cytolyt with no problems. It is just frustrating because we are doing nothing different that I can find.The next step is to see if the samples are coming from the same doctor/clinic and see if they are doing anything different. Martha -Original Message- From: Tom McNemar [mailto:tmcne...@lmhealth.org] Sent: Thursday, October 31, 2013 9:17 AM To: Martha Ward-Pathology; histonet@lists.utsouthwestern.edu Subject: RE: cell block staining issues We have not used B72.3 for quite awhile now but all of our NGYNs are either collected in or processed with Cytolyt and we have had no issues. Sorry, I know this isn't much help. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.org www.LMHealth.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Martha Ward-Pathology Sent: Thursday, October 31, 2013 8:19 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cell block staining issues We have something of a mystery here and I am hoping someone can help. My cytopath docs are saying that our B72.3 (TAG-72) antibody is not working well in cell blocks created from fluids (plural), etc. and that this is a fairly recent development (over the past 5-6 weeks or so).The surgical cases we have stained look great as does our control, which is placed on each slide. We have not changed lot numbers of antibody or made any up recently and the antibody is not expired, or even close.The only thing we can find that is different is that cytology has been rinsing the needles out with a product called Cytolyt, instead of saline and they started this around the first of August.Does anyone know if this product could cause any interference with our staining? The docs just say that the tumor cells are not staining and we are sort of at a loss here. Thanks in advance for your help. Martha Ward, MT (ASCP) QIHC Manager [Wake Forest Baptist Health] Molecular Diagnostics Lab Medical Center Boulevard \ Winston-Salem, NC 27157 p 336.716.2109 \ f 336.716.5890 mw...@wakehealth.edu This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] cell block staining issues
We have something of a mystery here and I am hoping someone can help. My cytopath docs are saying that our B72.3 (TAG-72) antibody is not working well in cell blocks created from fluids (plural), etc. and that this is a fairly recent development (over the past 5-6 weeks or so).The surgical cases we have stained look great as does our control, which is placed on each slide. We have not changed lot numbers of antibody or made any up recently and the antibody is not expired, or even close.The only thing we can find that is different is that cytology has been rinsing the needles out with a product called Cytolyt, instead of saline and they started this around the first of August.Does anyone know if this product could cause any interference with our staining? The docs just say that the tumor cells are not staining and we are sort of at a loss here. Thanks in advance for your help. Martha Ward, MT (ASCP) QIHC Manager [Wake Forest Baptist Health] Molecular Diagnostics Lab Medical Center Boulevard \ Winston-Salem, NC 27157 p 336.716.2109 \ f 336.716.5890 mw...@wakehealth.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Submitting Questions
I have had the same problem over the past few months... Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard \ Winston-Salem, NC 27157 p 336.716.2109 \ f 336.716.5890 mw...@wakehealth.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Nails, Felton Sent: Tuesday, September 24, 2013 2:58 PM To: 'Joanne Clark'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Submitting Questions I am in the same situation??? -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Joanne Clark Sent: Tuesday, September 24, 2013 12:54 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Submitting Questions Can anyone out in histoland explain to me why my submitted questions are not making it through to histonet? I submitted a question on the 19th of September, and I still have not seen it appear in the group emails I get daily. I sent the question to the correct address (I checked it to be sure). When I respond to someones question, I can see my responses, I just can't seem to get an original question submitted. Can anyone tell me what I am doing wrong? Disclaimer: This electronic message may contain information that is proprietary, confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. __ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Re: Minimum sample size for breast markers
Thanks to everyone that responded to my question. It has encouraged a nice discussion and I have learned from it. Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard \ Winston-Salem, NC 27157 p 336.716.2109 \ f 336.716.5890 mw...@wakehealth.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bob Richmond Sent: Thursday, September 12, 2013 1:56 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Minimum sample size for breast markers Pathologists need to be very ready to note that a breast cancer sample size is small, and that the markers need to be repeated on the lumpectomy or mastectomy specimen. This is easier said than done. Popping a Dolly-size breast into too-small container and dribbling a little formalin on it is not going to preserve the specimen adequately. Even with a moderate-sized lumpectomy specimen the formaldehyde penetration is going to be chancy. This is an issue that surgeons, OR nurses, and too many pathologists are unable to understand. The pathologist has to arrange to receive the specimen promptly and get the tumor cut into formalin. Easier said than done. The woman's cancer care depends on this getting done. It's non-trivial. And colon cancer is distinctly the next frontier for this problem. Bob Richmond Samurai Pathologist Maryville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Minimum sample size for breast markers
Thanks everyone From: Rene J Buesa [mailto:rjbu...@yahoo.com] Sent: Thursday, September 12, 2013 10:56 AM To: Martha Ward-Pathology; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Minimum sample size for breast markers No, there are no written instructions on the subject. You just have to state how many cells you found and the clinician is supposed to act accordingly. René J. From: Martha Ward-Pathology mailto:mw...@wakehealth.edu>> To: Rene J Buesa mailto:rjbu...@yahoo.com>>; "histonet@lists.utsouthwestern.edu<mailto:histonet@lists.utsouthwestern.edu>" mailto:histonet@lists.utsouthwestern.edu>> Sent: Thursday, September 12, 2013 10:24 AM Subject: RE: [Histonet] Minimum sample size for breast markers Thanks Rene. I realize that it is ultimately up to the Pathologist but I was wondering if there are any written guideline/suggestions because I have had pathologists ask me if there is minimum. From: Rene J Buesa [mailto:rjbu...@yahoo.com] Sent: Thursday, September 12, 2013 9:23 AM To: Martha Ward-Pathology; histonet@lists.utsouthwestern.edu<mailto:histonet@lists.utsouthwestern.edu> Subject: Re: [Histonet] Minimum sample size for breast markers I do not think this is the adequate forum to ask this question because you may get a wrong answer that could determine your decision. Your pathologist is the one who should decide about this issue. René J. From: Martha Ward-Pathology mailto:mw...@wakehealth.edu>> To: "histonet@lists.utsouthwestern.edu<mailto:histonet@lists.utsouthwestern.edu>" mailto:histonet@lists.utsouthwestern.edu>> Sent: Thursday, September 12, 2013 9:06 AM Subject: [Histonet] Minimum sample size for breast markers Is there a minimum number of cells that should be present when testing for ER, PR and Her2? I have looked on the CAP website but cannot see anything about this. We are constantly getting these small biopsies or FNAs for testing and I sometimes wonder what the lowest threshold is for testing...20 tumor cells, 50 tumor cells Any information would be appreciated. Thanks in advance for your help. Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard \ Winston-Salem, NC 27157 p 336.716.2109 \ f 336.716.5890 mw...@wakehealth.edu<mailto:mw...@wakehealth.edu> ___ Histonet mailing list Histonet@lists.utsouthwestern.edu<mailto:Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Minimum sample size for breast markers
Thanks Rene. I realize that it is ultimately up to the Pathologist but I was wondering if there are any written guideline/suggestions because I have had pathologists ask me if there is minimum. From: Rene J Buesa [mailto:rjbu...@yahoo.com] Sent: Thursday, September 12, 2013 9:23 AM To: Martha Ward-Pathology; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Minimum sample size for breast markers I do not think this is the adequate forum to ask this question because you may get a wrong answer that could determine your decision. Your pathologist is the one who should decide about this issue. René J. From: Martha Ward-Pathology mailto:mw...@wakehealth.edu>> To: "histonet@lists.utsouthwestern.edu<mailto:histonet@lists.utsouthwestern.edu>" mailto:histonet@lists.utsouthwestern.edu>> Sent: Thursday, September 12, 2013 9:06 AM Subject: [Histonet] Minimum sample size for breast markers Is there a minimum number of cells that should be present when testing for ER, PR and Her2? I have looked on the CAP website but cannot see anything about this. We are constantly getting these small biopsies or FNAs for testing and I sometimes wonder what the lowest threshold is for testing...20 tumor cells, 50 tumor cells Any information would be appreciated. Thanks in advance for your help. Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard \ Winston-Salem, NC 27157 p 336.716.2109 \ f 336.716.5890 mw...@wakehealth.edu<mailto:mw...@wakehealth.edu> ___ Histonet mailing list Histonet@lists.utsouthwestern.edu<mailto:Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Minimum sample size for breast markers
Is there a minimum number of cells that should be present when testing for ER, PR and Her2? I have looked on the CAP website but cannot see anything about this. We are constantly getting these small biopsies or FNAs for testing and I sometimes wonder what the lowest threshold is for testing...20 tumor cells, 50 tumor cells Any information would be appreciated. Thanks in advance for your help. Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard \ Winston-Salem, NC 27157 p 336.716.2109 \ f 336.716.5890 mw...@wakehealth.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] EPIC Beaker
I would be interested in any information as well. We do not plan to implement for a year or more but one of our hospitals is working on Beaker now and finding that it does not adapt to AP as well as it does CP. Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard \ Winston-Salem, NC 27157 p 336.716.2109 \ f 336.716.5890 mw...@wakehealth.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Pam Marcum Sent: Wednesday, September 04, 2013 10:16 AM To: Histonet Subject: [Histonet] EPIC Beaker I know asked this a couple of months ago however; we are just getting ready to work with the EPIC people on the Histology/IHC/Slide & Block/Gross Room issues we need in AP. I am looking for any advise or recommendations anyone who is running Epic Beaker now or is in the process as we are to implement it in the next 6 months or so. I would prefer this is off line if anyone resonding is OK doing it that way. If others want the info then please to Histonet will work too. We are having issues getting our IT group and Epic to understand AP is not the same as CP. Thank You, Pam Marcum UAMS Histology Supervisor ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Unstained slides
We are looking at ways to improve our work processes, save time and labor and reduce costs, all while maintaining patient quality...as we all are of course. During our conversations the subject of cutting unstained slides has come up and we are looking for bench marking data to see if we are where we need to be. Currently we are cutting unstained slides for various protocols (including prostate biopsies), where the specimens are tiny, but we are also cutting a lot of "just in case" unstained slides. Our research has shown that about 50% of the time the unstained slides requested are never used and we are trying to find out if that is high, low or about what other institutions are seeing. If anyone has any data they could share with us we would appreciate it. Thanks in advance for your help. I can share what I find out if others are interested. Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard \ Winston-Salem, NC 27157 p 336.716.2109 \ f 336.716.5890 mw...@wakehealth.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Immunofluorescence on paraffin sections
We do quite a number of renal biopsies and sometimes the frozen tissue is not sufficient for the immunofluorescent panel and we have to use paraffin sections for our fluorescence staining. It has never really worked all that well and so we are looking for alternative methods. We get all our FITC labeled antibodies from Dako and would like to use them on our Bond III stainer, not for immunofluorescence but for immunoperoxidase staining. Has anyone out in Histoland tried this on the Bond, and if so, would you share your procedure with me? Thanks in advance for any help you can give me. Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard \ Winston-Salem, NC 27157 p 336.716.2109 \ f 336.716.5890 mw...@wakehealth.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] DAB enhancer
Sorry but I cannot get my messages to go through to the Histonet so I am piggybacking onto a previous message: I have been asked by a new pathologist to look into a DAB enhancer for use on our Bond III instruments. Are many of you using this on the Bond, or any other staining platform and what are the pros and cons of its use? Thanks! Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard \ Winston-Salem, NC 27157 p 336.716.2109 \ f 336.716.5890 mw...@wakehealth.edu -Original Message- ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] DAB enhancer
I have been asked by a new pathologist to look into a DAB enhancer for use on our Bond III instruments. Are many of you using this on the Bond, or any other staining platform and what are the pros and cons of its use? Thanks! Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard \ Winston-Salem, NC 27157 p 336.716.2109 \ f 336.716.5890 mw...@wakehealth.edu Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] p16 on the Bond
I am having trouble posting to the histonet for some reason. I am looking for condition and incubation times for p16 on the Bond. Our new pathologist says our p16 looks overstained to him and not at all what he is accustomed to seeing and I am trying to get the staining more to his liking, without much success. Thanks in advance for the help. Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard \ Winston-Salem, NC 27157 p 336.716.2109 \ f 336.716.5890 mw...@wakehealth.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Billing ???
At our institution the hospital bills the technical and the pathologist bills the professional. Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard \ Winston-Salem, NC 27157 p 336.716.2109 \ f 336.716.5890 mw...@wakehealth.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of jmasla...@stpetes.org Sent: Friday, June 21, 2013 11:49 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Billing ??? Happy Friday This is a question to those who work at a hospital that have contracted pathologists. Does your hospital bill the technical component and the pathologist bill the profession component or does the pathologist bill everything and pay the hospital for technical component? Joe Maslanka BS, CT,HT (ASCP) Anatomical Pathology Technical Supervisor Give thanks for ALL things. "Kindness is the language the blind can see & the deaf can hear- Mark Twain This electronic mail message contains information which is confidential. If you are not the intended recipient, please be aware that any disclosure, photocopying, distribution or use of the contents of the received information is prohibited. If you have received this e-mail in error, please reply to the sender immediately and permanently delete this message and all copies of it. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: negative controls
We eliminated them on August 1st, except in cases where they are specifically requested. So far we have run <10 negative slides. Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard \ Winston-Salem, NC 27157 p 336.716.2109 \ f 336.716.5890 mw...@wakehealth.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V Sent: Friday, August 17, 2012 12:36 PM To: 'Romundstad, Pamela K'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: negative controls For everyone performing immunos are you not running negative controls now with this new ruling from CAP? Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1302 office | 501.364.1241 fax hor...@archildrens.org archildrens.org 100 YEARS YOUNG! JOIN THE PARTY AT ach100.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Romundstad, Pamela K Sent: Friday, August 17, 2012 10:25 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Histonet Digest, Vol 105, Issue 20 Yes, the negative control updated change is in effect as of July 31. 2012. I contacted CAP personally this week and was directed to the revised ANP.22570. It only applies to polymer based detection systems (biotin-free). Respectfully, Pamela Romundstad HT, QIHC Gundersen Lutheran 608-775-3139 From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of histonet-requ...@lists.utsouthwestern.edu [histonet-requ...@lists.utsouthwestern.edu] Sent: Thursday, August 16, 2012 12:02 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 105, Issue 20 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. protocol for EGFP IHC in frozen rat brains (Bass, Caroline) 2. Re: Two identifiers... (Bob Richmond) 3. RE: Re: Two identifiers... (joelle weaver) 4. RE: Re: Two identifiers... (Michael Mihalik) 5. Negative Control (Pardue, Judith) 6. RE: Special Stain documentation (hymclab) 7. Peloris (Pardue, Judith) 8. RE: RE: Two identifiers... (Pratt, Caroline) 9. RE: RE: Two identifiers... (Tom McNemar) 10. marker specific for mouse fibroblasts (Kim Merriam) 11. RE: RE: Two identifiers... (Pratt, Caroline) 12. RE: marker specific for mouse fibroblasts (Elizabeth Chlipala) -- Message: 1 Date: Wed, 15 Aug 2012 18:07:46 -0400 From: "Bass, Caroline" Subject: [Histonet] protocol for EGFP IHC in frozen rat brains To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="Windows-1252" Hello Everyone, I have some rat brains that should express GFP, the brains were collected fresh and snap frozen in dry ice/isopentane. They have been stored for several months in a -80. I'd like to collect 500 um sections, thaw mount them to a slide an reserve these for mRNA collection. I would then like to take intervening sections to stain for GFP. I have a staining protocol for perfused, formalin fixed, sucrose protected floating brain sections that works fine. I'm a little more concerned about moving to unfixed sections. I know I need to cut everything on a cryostat and I assume that I have to process the thinner sections on the slide instead of free floating. What is the best way to proceed? Here are some specific questions? 1) what thickness should I cut, 50 um works great for my fixed sections. 2) should I "fix" these sections by either paraformaldehyde vapors or dunking in fix? If so, what kind/concentration? 3) could I then proceed as though this were a normal GFP immuno, with DAB stain? 4) should I used plus slides or subbed slides? 5) will the sections stay on without fixation? Any and all suggestions would be appreciated! Thanks, Caroline -- Message: 2 Date: Wed, 15 Aug 2012 21:11:12 -0400 From: Bob Richmond Subject: [Histonet] Re: Two identifiers... To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1
RE: [Histonet] Negative Reagent Control
What wonderful news Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard \ Winston-Salem, NC 27157 p 336.716.2109 \ f 336.716.5890 mw...@wakehealth.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Angela Bitting Sent: Wednesday, July 18, 2012 7:26 AM To: Richard Cartun; Histonet Subject: Re: [Histonet] Negative Reagent Control Our Prayers have been answered!!! >>> Richard Cartun 7/17/2012 7:04 PM >>> Most of you know that I have been advocating for the elimination of the "Negative Reagent Control" when using a non-avidin-biotin detection system (polymer) in immunohistochemical testing. In my opinion, it is a waste of healthcare dollars and, more importantly, precious patient specimen. Last year at the NSH IHC Forum in Denver I met James Dvorak, MT(ASCP) from the College of American Pathologists. We had a long discussion about this and he agreed to support my position within CAP. With the support of James, and the help of Dr. Regan Fulton (from the CAP IHC Committee), the wording on the CAP Anatomic Pathology checklist for question ANP.22570 will be changed. The new wording includes the following sentence: "Immunohistochemical tests using polymer-based detection systems (biotin-free) are sufficiently free of background reactivity to obviated the need for a negative reagent control and such controls may be omitted at the discretion of the laboratory director." I announced this change at the 2012 NSH IHC/ISH Forum this past weekend in Windsor, CT to "loud applause". This one change stands to save the healthcare system millions of dollars. Thank you Jim and Dr. Fulton for making this happen! Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Beaker module in EPIC
So far we have not had any responses so I assume the answer is no. Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard \ Winston-Salem, NC 27157 p 336.716.2109 \ f 336.716.5890 mw...@wakehealth.edu -Original Message- From: Michael Mihalik [mailto:m...@pathview.com] Sent: Friday, July 13, 2012 2:03 PM To: Martha Ward-Pathology; 'Paula Sicurello'; 'HistoNet'; microsc...@microscopy.com Subject: RE: [Histonet] Beaker module in EPIC I'm curious. Are ANY labs using Epic for their APLIS? The last I heard was a sales presentation a couple of years ago where the sales person declined to show the APLIS "because it wasn't ready". I do know of hospitals using Epic for order entry, though. Michael Mihalik PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 952.241.7369 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Martha Ward-Pathology Sent: Friday, July 13, 2012 7:42 AM To: Paula Sicurello; HistoNet; microsc...@microscopy.com Subject: RE: [Histonet] Beaker module in EPIC Again, we would be interested in any replies as well. Our institution is going live with Epic this fall but the labs will not be using Beaker until later on. We are still trying to figure out how all this will work and welcome all information. Paula, how soon will Duke be up and using Beaker? Martha Ward Wake Forest Baptist Health 336-716-2109 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Paula Sicurello Sent: Thursday, July 12, 2012 4:05 PM To: HistoNet; microsc...@microscopy.com Subject: [Histonet] Beaker module in EPIC Hello all Listers, I've already asked the Histonet but I'm wondering about a different thing this time. Does anyone have experience with Beaker in conjunction with Anatomic Pathology, Surgical Pathology or the multiple other clinical labs that are in hospitals today? I work in Anatomic/Surgical Pathology which encompasses all the Clinical Laboratory services here at Duke. We have been told to determine what we need for the Beaker module to be useful to us. I've seen a brief demo. as to how it will work for the ordering clinicians (they can order every test STAT!). Please, send me any of the pluses or minuses that you've experienced using Beaker. Thanks, Paula :-) -- Paula Sicurello, HTL (ASCP) Supervisor, Clinical Electron Microscopy Laboratory Duke University Health System Rm.#251M, Duke South, Green Zone Durham, North Carolina 27710 P: 919.684.2091 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Beaker module in EPIC
Again, we would be interested in any replies as well. Our institution is going live with Epic this fall but the labs will not be using Beaker until later on. We are still trying to figure out how all this will work and welcome all information. Paula, how soon will Duke be up and using Beaker? Martha Ward Wake Forest Baptist Health 336-716-2109 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Paula Sicurello Sent: Thursday, July 12, 2012 4:05 PM To: HistoNet; microsc...@microscopy.com Subject: [Histonet] Beaker module in EPIC Hello all Listers, I've already asked the Histonet but I'm wondering about a different thing this time. Does anyone have experience with Beaker in conjunction with Anatomic Pathology, Surgical Pathology or the multiple other clinical labs that are in hospitals today? I work in Anatomic/Surgical Pathology which encompasses all the Clinical Laboratory services here at Duke. We have been told to determine what we need for the Beaker module to be useful to us. I've seen a brief demo. as to how it will work for the ordering clinicians (they can order every test STAT!). Please, send me any of the pluses or minuses that you've experienced using Beaker. Thanks, Paula :-) -- Paula Sicurello, HTL (ASCP) Supervisor, Clinical Electron Microscopy Laboratory Duke University Health System Rm.#251M, Duke South, Green Zone Durham, North Carolina 27710 P: 919.684.2091 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Epic Beaker module
We would interested in any replies as well. Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard \ Winston-Salem, NC 27157 p 336.716.2109 \ f 336.716.5890 mw...@wakehealth.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Paula Sicurello Sent: Thursday, July 12, 2012 8:54 AM To: HistoNet Subject: [Histonet] Epic Beaker module Good Morning One and All, Does anyone out there have any experience with the Beaker module of the LIS system EPIC? We have tests that require tables and charts to be inserted into the final report, pathologists need to have a case queue and other items. If you are using Epic/Beaker- please let me know if your experiences and what if anything have you had Epic build into it for you. Thanks, Paula -- Paula Sicurello, HTL (ASCP) Supervisor, Clinical Electron Microscopy Laboratory Duke University Health System Rm.#251M, Duke South, Green Zone Durham, North Carolina 27710 P: 919.684.2091 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Napsin A
Hello. I was wondering if anyone had used the NapsinA from BioCare on the Bond III and if so, would you mind sharing your protocol? Thanks in advance for any advice you can give. Martha Ward, MT (ASCP) QIHC Manager, Molecular Diagnostics Lab Dept. of Pathology Wake Forest University Baptist Medical Center Winston-Salem, NC 27157 336-716-2109 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] NCCI policy on IHC billing
>From what we understand from the new policy the cocktailed antibodies, such as >PIN4, are not to be charged separately. Martha Ward, MT (ASCP) QIHC Manager, Molecular Diagnostics Lab Dept. of Pathology Wake Forest University Baptist Medical Center Winston-Salem, NC 27157 336-716-2104 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rae Staskiewicz Sent: Thursday, January 05, 2012 9:00 AM To: 'Clare Thornton'; 'Sally Price'; histonet@lists.utsouthwestern.edu Cc: Dana Spears Subject: RE: [Histonet] NCCI policy on IHC billing Clair, I agree that the cocktailed antibodies would be charged as a single test, however, with the dual and triple stains, since these are technically done with separate procedures, would not each antibody be able to be charged? This is what we are thinking. How are others interpreting this Rae Staskiewicz -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Clare Thornton Sent: Thursday, January 05, 2012 5:35 AM To: Sally Price; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] NCCI policy on IHC billing Sally, The way I read it, I don't think it's one antibody per specimen; I think what they are saying is that if you are running one antibody on multiple blocks from one specimen, you can only bill once for that antibody. I think if you are running a panel of antibodies one one block from a specimen, you can still charge for each antibody. However, I completely agree with you. This change came as a big surprise for our lab, until Dorothy posted it on Histonet we had no idea. How can that be? (And thanks, Dorothy!) The day that post came out we happened to be running 20 pankeratin/p63 double stain slides, all on multiple blocks from one specimen. The two antibodies from that double stain are applied at different times (to the same slide) and we use two different detections. So had we ran them after the new year, we would've been able to charge only once for those 20 slides, never mind 20 tests being used from each antibody and 20 tests being taken from two different detection kits. And the tech time put into cutting those 20 slides! We have several double and triple stains that we run on a daily basis, and only one component from the triple stain is a cocktail; the rest are separate antibodies being applied to the same slide using two different detections. I'm not sure exactly what our lab is going to do about it, but somehow this change should have been made aware to everyone well before it happened. There are going to be labs who are no longer in compliance, and we all know what that means.. Clare J. Thornton, HTL(ASCP) Assistant Histology Supervisor Dahl-Chase Diagnostic Services 417 State Street, Suite 540 Bangor, ME 04401 cthorn...@dahlchase.com From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sally Price [sprice2...@gmail.com] Sent: Thursday, January 05, 2012 12:16 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] NCCI policy on IHC billing Histonetters: I waited a few days to see how others might weigh in after this information was posted. Call me crazy, but I expected quite a bit more reaction from our community. How is it that such a signifcant change in how IHC testing may be conducted and will be paid for in the future can produce so little response? The way this new policy is stated, it looks pretty straightfoward: one antibody (IHC procedure) per specimen; so, when it's necessary to use a battery of IHC stains to determine the origin of an undifferentiated neoplasm, the lab can only bill for one procedure. How could such an approach be possible? And what about multi-antibody procedures, which are usually more cost effective than single-antibody procedures? Come on folks, this is a big deal becuase IHC staining is essential to to the practice of anatomic pathology and provides a lot of us with our livelihood. I know I'm not alone in thinking that the CMS needs to know that this new policy is completely impractical and must be changed. Sure, there's some unnecessary IHC procedures being performed, but this isn't the way limit the problem. Sally -- Message: 6 Date: Fri, 30 Dec 2011 12:33:17 -0600 From: "Webb, Dorothy L" Subject: [Histonet] NCCI policy update To: "'histonet@lists.utsouthwestern.edu'" Is everyone aware that beginning 1/1/12, we can no longer bill for each block regarding IHC billing, only one unit of billing for each part type no matter how many blocks are stained? Also IHC "cocktail" stains, such as PIN4 must now be billed as one unit even though multiple antibodies are reported out. Kind of a s
[Histonet] RE: Epic LIS
I would also be interested in any replies concerning EPIC-beaker. Martha Ward, MT (ASCP) QIHC Manager, Molecular Diagnostics Lab Dept. of Pathology Wake Forest University Baptist Medical Center Winston-Salem, NC 27157 336-716-2104 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of jessica.va...@hcahealthcare.com Sent: Wednesday, January 04, 2012 1:29 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Epic LIS Is anyone currently using the EPIC-beaker LIS system? Can you contact me if you are? Thanks Jessica Vacca ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: NCCI policy update
Oh yes, we are very aware and quite upset at the change! Martha Ward, MT (ASCP) QIHC Manager, Molecular Diagnostics Lab Dept. of Pathology Wake Forest University Baptist Medical Center Winston-Salem, NC 27157 336-716-2104 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Webb, Dorothy L Sent: Friday, December 30, 2011 1:33 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] NCCI policy update Is everyone aware that beginning 1/1/12, we can no longer bill for each block regarding IHC billing, only one unit of billing for each part type no matter how many blocks are stained? Also IHC "cocktail" stains, such as PIN4 must now be billed as one unit even though multiple antibodies are reported out. Kind of a surprising reversal of the policy set in motion 10/1/2009. SPECIMEN becomes the unit of service rather than block(s) for IHC codes 88342, 88360, and 88361. Happy New Year to everyone out there. May 2012 find you happiness and health! Dorothy Webb, HT Regions Histology TS 651-254-2962 This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] C4d immunofluorescent positive controls
We are interested in finding out what type of positive control tissue everyone is using when performing C4d immunofluorescence on heart biopsies. Thanks in advance for any and all responses. Martha Ward, MT (ASCP) QIHC Manager, Molecular Diagnostics Lab Dept. of Pathology Wake Forest University Baptist Medical Center Winston-Salem, NC 27157 336-716-2104 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] PAX 2 antibody
Hello all, I am still hunting for a source for the PAX 2 antibody.Is this antibody currently available? I have heard in the past that there was a manufacturing issue. Martha Ward, MT (ASCP) QIHC Manager, Molecular Diagnostics Lab Dept. of Pathology Wake Forest University Baptist Medical Center Winston-Salem, NC 27157 336-716-2104 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] MHC-class 1 antibody
Thank you to the people who responded to my inquiry about the IDH1 antibody. I think we have found one to try. I have also have a request for the MHC-Class 1 antibody for muscle biopsies. Could anyone performing this suggest a vendor. So far I am coming up with antibodies for frozen tissue but they are looking for an antibody for paraffin embedded tissues. Any help would be appreciated. Hope everyone has a good Thanksgiving holiday. Martha Ward, MT (ASCP) QIHC Manager, Molecular Diagnostics Lab Dept. of Pathology Wake Forest University Baptist Medical Center Winston-Salem, NC 27157 336-716-2104 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] IDH1 and IDH2 antibodies, MHC-Class I
Hello all, I have had a request from our neuropathologists to bring these antibodies in house but I am having some trouble finding vendors for them. I would appreciate any information. Thanks! Martha Ward, MT (ASCP) QIHC Manager, Molecular Diagnostics Lab Dept. of Pathology Wake Forest University Baptist Medical Center Winston-Salem, NC 27157 336-716-2104 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Leica Bond III
We sometimes have issues with EBER and more often, with the Kappa and Lambda ISH on the Bond 3, more specifically on our tissues that were fixed in B Plus rather than formalin. I would interested in the responses as well. Martha Ward, MT (ASCP) QIHC Manager, Molecular Diagnostics Lab Dept. of Pathology Wake Forest University Baptist Medical Center Winston-Salem, NC 27157 336-716-2104 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Nails, Felton Sent: Friday, September 02, 2011 10:51 AM To: 'diane.cr...@amcny.org'; histonet@lists.utsouthwestern.edu Subject: [Histonet] Leica Bond III I want to poll Leica Bond III users to see if they are able to successfully run EBER Insitu on their unit? We continue to get inconsistent staining and inconsistent background staining. I have been told it's a pH problem, it's a tissue processing problem but we are able to do the stain with great results by hand. I welcome any and all suggestions __ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. __ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Test email after email address change
Martha Ward, MT (ASCP) QIHC Manager, Molecular Diagnostics Lab Dept. of Pathology Wake Forest University Baptist Medical Center Winston-Salem, NC 27157 336-716-2104 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] C FLIP AND NKF Beta antibodies
I have been asked about a research project involving these two antibodies and we are having some trouble finding sources that are appropriate for formalin fixed, paraffin embedded tissues. Does anyone have experience with these antibodies? Martha Ward, MT (ASCP) QIHC Assistant Manager Molecular Diagnostics Lab Dept. of Pathology Wake Forest University Baptist Medical Center Winston-Salem, NC 27157 336-716-2104 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Lab humidity range
We chose 10 - 90% as our range after reading what few instrument manuals even addressed the issue. I can only wish we could get to 17% humidity here in NC. Martha Ward, MT (ASCP) QIHC Assistant Manager Molecular Diagnostics Lab Dept. of Pathology Wake Forest University Baptist Medical Center Winston-Salem, NC 27157 336-716-2104 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Thursday, July 14, 2011 2:23 PM To: Histonet@lists.utsouthwestern.edu Cc: Hanosh, Timothy J.; Jillson, Gregory Subject: [Histonet] Lab humidity range We're prepping for a Big Inspection (I could tell you who but then I'd have to enter the Witness Protection Program). The Inspecting Organization will want me to cite a range for HUMIDITY in the lab. Because this not only hinges on our weather (we're in Monsoon Season right now and the lab humidity is 47% as I write but is normally between 17-20%), but also MUST be based on any of the histology equipment that may be affected by humidity in any way. Realizing that we all work in different "environments" and usually have little or no control over the HVAC system, what did you decide upon as your HUMIDITY RANGE for the SOP under which you operate? If there are no specific equipment requirements, I will be able to give a range of 0-100% without the necessity for a Corrective Action Report. If I am too specific and give too narrow a range, I may end up filling out a Corrective Action Report for days when I'm "out of range". Please give me as much detail as you are able, without being required to join me in hiding. Your input will be rewarded (don't ask). Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Langerin antibody
Hi all! I have been asked by one of our Pathology fellows to see if anyone is performing this antibody-Langerin- and if so, would you be willing to perform it for us.It is not something that we would be adding to our test menu at this time. Please let me know the fee and how many slides you would need. Thanks in advance for your help. Martha Ward, MT (ASCP) QIHC Assistant Manager Molecular Diagnostics Lab Dept. of Pathology Wake Forest University Baptist Medical Center Winston-Salem, NC 27157 336-716-2104 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] cost to produce one block
I am posting this question for a co-worker. She is attempting to calculate the cost of producing one formalin-fixed, paraffin embedded block. She has the amount of personnel time it takes but is having trouble with the reagents, etc. While we know there are lots of variables she is wondering if anyone would be willing to share this information with her. She is getting differing numbers and is trying to figure out which amount is most correct.Any responses will be kept confidential. Thanks! Martha Ward, MT (ASCP) QIHC Assistant Manager Molecular Diagnostics Lab Dept. of Pathology Wake Forest University Baptist Medical Center Winston-Salem, NC 27157 336-716-2104 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Thanks for the hair processing information
Thanks so much to the folks that responded to my request for information. I forwarded them on to my colleague. Martha Ward, MT (ASCP) QIHC Assistant Manager Molecular Diagnostics Lab Dept. of Pathology Wake Forest University Baptist Medical Center Winston-Salem, NC 27157 336-716-2104 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Processing hair
I am posting this question for a colleague who has been approached to do a research project involving processing human hair. Has anyone done this, and if so, could you provide the procedure. Thanks in advance for your help. Martha Ward, MT (ASCP) QIHC Assistant Manager Molecular Diagnostics Lab Dept. of Pathology Wake Forest University Baptist Medical Center Winston-Salem, NC 27157 336-716-2104 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Direct costs for small biopsy processing
I am posted this question for a colleague.She has been asked to provide the direct costs for processing one biopsy, for example, a punch skin biopsy. Would anyone be willing to share that information with us? She is looking at the total costs of having a resident or PA gross, someone to accession the case, the tissue processing, embedding, microtomy, staining the H&E, coverslipping and reviewing the slide. We know that reagents and salary costs will vary but she just wants a ball park figure for comparison.Thanks in advance for your help. Martha Ward, MT (ASCP) QIHC Assistant Manager Molecular Diagnostics Lab Dept. of Pathology Wake Forest University Baptist Medical Center Winston-Salem, NC 27157 336-716-2104 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] SOX11 antibody
Hello all, I have been asked to try to work up the SOX11 antibody for a research project. The antibody they gave me is from Sigma Life Science. I am having difficulty getting any staining at all. If anyone has any suggestions I would be very grateful.We are using a Leica Bond 3. Thanks in advance for any help. Martha Ward, MT (ASCP) QIHC Assistant Manager Molecular Diagnostics Lab Dept. of Pathology Wake Forest University Baptist Medical Center Winston-Salem, NC 27157 336-716-2104 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: negative controls on immunos
We do not run a second negative control. I agree that it wastes reagents and precious tissue. Martha Ward Wake Forest University Baptist Medical Center -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Diana McCaig Sent: Tuesday, March 01, 2011 1:08 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] negative controls on immunos If you do a run of several immunos today and you run a negative control, and there is a request for additional immunos tomorrow, would you run another negative control with the additional slides. They are being stained on a stainer and not manually, diana ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Her2 requests on decal specimens
I am hoping to get some guidance on this. Occasionally we get requests to perform HER2 on specimens that have been decaled. The revised CAP question, ANP.22998 specifically states in the notes that decalcification with strong acids should not be used. How are other institutions handling this? Martha Ward, MT (ASCP) QIHC Assistant Manager Molecular Diagnostics Lab Dept. of Pathology Wake Forest University Baptist Medical Center Winston-Salem, NC 27157 336-716-2104 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] IHC validation
What a sensible process! I agree with your approach. Martha Ward, MT (ASCP) QIHC Assistant Manager Molecular Diagnostics Lab Dept. of Pathology Wake Forest University Baptist Medical Center Winston-Salem, NC 27157 336-716-2104 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Wednesday, February 09, 2011 3:27 PM To: Histonet; Joe Nocito Subject: Re: [Histonet] IHC validation Joe: This is what I would do; 1- run 1 control slide per antibody you have in your "arsenal" 2- compare the result with a control slide already in your files. 3- show both slides to the chief pathologist (after all is his/her opinion the one is going to be asked by CAP) 4- those antibodies whose positive controls reacted substantially different to those in your files are the ones you have to work with with respect to concentration or detection method. 5- never overdue it, and avoid excessive costs that usually are never appreciated. Rely always in your pathologist's opinion René J. --- On Tue, 2/8/11, Joe Nocito wrote: From: Joe Nocito Subject: [Histonet] IHC validation To: "Histonet" Date: Tuesday, February 8, 2011, 5:43 PM Greetings Histoland, I need some help. We are about to switch IHC machines from the Richard-Allen Axiom to the Ventana Benchmark Ultra. How many slides, per antibody, do you run for the validation study? We have over 100 primary antibodies. Normally, when we work up a new antibody, we start with a titer. Once that is established, we run 10 cases to check for specificity. Hopefully we can obtain cases that are really positive, some weakly positive and some flat out negative. Once that is completed, we run 10 different tissue types to check for any unexpected cross-reactivity. The ultra holds 30 slides and we are receiving two machines. If we run 10 slides/antibody, that's going to take a while, not to mention the number of detection kits that will be used. Do you think 5 slides/antibody is sufficient? I emailed CAP last week for their take and they never returned my email (I told my medical director to hold their check for the year and see how fast they respond to that). Ah oh, don't go down that road Joe, it's unhealthy. What are your thoughts? Thanks Joe (JTT) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] High Complexity Testing
I completely agree!!! Martha Ward, MT (ASCP) QIHC Assistant Manager Molecular Diagnostics Lab Dept. of Pathology Wake Forest University Baptist Medical Center Winston-Salem, NC 27157 336-716-2104 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Dawson, Glen Sent: Tuesday, February 08, 2011 1:40 PM Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] High Complexity Testing Alright, if IHC is not high complexity testing, CAP should cut that massive part of their inspection in half and concentrate more on the pathologists' ability to accurately interpret the staining. Too much CAP regulation, Proficiency Testing & validation requirements involved if all IHC is is part of "Processing". My Opinion, Glen A. Dawson Milwaukee, WI -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Victoria Baker Sent: Tuesday, February 08, 2011 12:17 PM To: Whitaker, Bonnie Cc: Horn, Hazel V; Goins, Tresa; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] High Complexity Testing I should not have included CLIA in my e-mail as it would seem it has clouded things a little. I do apologize. Initially when these issues and guidelines came about CLIA and CAP dovetailed as far as Histology was concerned. Shelia you were looking for contacts that would help you with getting a more solid base to meet these regulations. If you go to the CAP website and click on the IHC link you will find links and publications to assist you. I would recommend that you contact the Applied Immunohistochemistry society as well. NSH or your state/regional society may also have additional information. Should I see something else in my searches I will most willingly forwarded them to you. Vikki On Tue, Feb 8, 2011 at 12:43 PM, Whitaker, Bonnie wrote: > Hi All, > > There is a difference in performing a task (immunostaining) that is > complex, and performing "high complexity testing" as the CLIA > regulations govern. > > Yes, staining is a complex task, and it requires knowlegable techs to > ensure that it is properly done, and to troubleshoot difficulties > when necessary. > > It is "high complexity testing" because "testing personnel" in > anatomic pathology are pathologists (and the non-physician people > performing gross examinations, who must meet "high complexity testing > personnel" > requirements. > > "Testing personnel" as defined by CLIA, are the people that report > results of that test, not people who perform other related duties. > > That's my explanation of the whole mess. > > Bonnie Whitaker > AP Operations Director > Ohio State University Medical Center > Department of Pathology > 614.293.5048 > > > -Original Message- > From: histonet-boun...@lists.utsouthwestern.edu [mailto: > histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Goins, Tresa > Sent: Tuesday, February 08, 2011 12:22 PM > To: Horn, Hazel V; 'Rene J Buesa'; histonet@lists.utsouthwestern.edu; > Sheila Fonner > Subject: RE: [Histonet] High Complexity Testing > > I must disagree with this assessment of what makes a test complex. If > the test is done properly [the responsibility of the technologist] > then the reading to the test is a visual determination that requires > experience on the part of the pathologist, but if the test is not done > properly, will the pathologist be able to tell the technologist what to do to > fix the problem? > > Where's the Tylenol? > > > -Original Message- > From: histonet-boun...@lists.utsouthwestern.edu [mailto: > histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V > Sent: Tuesday, February 08, 2011 9:58 AM > To: 'Rene J Buesa'; histonet@lists.utsouthwestern.edu; Sheila Fonner > Subject: RE: [Histonet] High Complexity Testing > > While the test is high complexity it is the READING of the test by the > pathologist that determines its complexity. Because histotechs do not > report the results our part of this test is not high complexity. > > Hazel Horn > Hazel Horn, HT/HTL (ASCP) > Supervisor of Autopsy/Histology/Transcription Arkansas Children's Hospital > 1 Children's WaySlot 820 > Little Rock, AR 72202 > > phone 501.364.4240 > fax501.364.3155 > > visit us on the web at:www.archildrens.org > > -Original Message- > From: histonet-boun...@lists.utsouthwestern.edu [mailto: > histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa > Sent: Tuesday, February 08, 2011 10:32 AM > To: histonet@lists.utsouthwestern.edu
[Histonet] CAP question ANP.22970
We are having our inspection this spring and I am working to get all our procedures, etc. ready. I am having trouble finding benchmark information for comparison for HER2 to comply with this question - ..."the laboratory at least annually compares its patient results with published benchmarks," We are using the Dako Herceptest. I spoke with Dako tech services and they did not have any information. What are other labs using for a benchmark. Thanks in advance for all your help. Martha Ward, MT (ASCP) QIHC Assistant Manager Molecular Diagnostics Lab Dept. of Pathology Wake Forest University Baptist Medical Center Winston-Salem, NC 27157 336-716-2104 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Manual Coverslipping Safety Issues
Yes we actually used the exposure to xylene to justify the purchase of a coverslipper in our lab. Ventilation in our lab is very poor to non-existent and we were successful in our push for the coverslipper. I also had an employee who was having difficulties with the fumes, etc. It has made a world of difference. Good luck! Martha Ward, MT (ASCP) QIHC Assistant Manager Molecular Diagnostics Lab Dept. of Pathology Wake Forest University Baptist Medical Center Winston-Salem, NC 27157 336-716-2104 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Gagnon, Eric Sent: Tuesday, January 04, 2011 12:24 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Manual Coverslipping Safety Issues Has anyone successfully lobbied their institution for an automated coverslipper for safety reasons? Still coverslipping manually-stained IHC, neuro autopsy and special stains, sometimes hundreds per day. There has to be a better way. Under budget constraints. That's why I'm wondering if anyone has used concerns about histology staff safety, specifically techs under direct exposure to toluene/xylene, to enable purchase of an automated/robot coverslipper. I'd be interested in anyone's experience with this approach, successfully or unsuccessfully. Eric Gagnon MLT Histology Laboratory Kingston General Hospital Kingston, Ontario, Canada ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Fisher MicroProbe manual staining system
Does anyone have one of these systems that they would be willing to donate? One of our pathologists is going on a mission trip to Honduras and would like to take one of these down there with him. We used to have the set up but recently got rid of it. (Timing is everything!!) Martha Ward, MT (ASCP) QIHC Assistant Manager Molecular Diagnostics Lab Dept. of Pathology Wake Forest University Baptist Medical Center Winston-Salem, NC 27157 336-716-2104 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Physician Signatures
Like everyone else we are struggling with how to get everyone to comply. If anyone has any plans I think the whole group could benefit. Right now our institution is getting together to figure out a way to get the information out to the surgeons, etc but no concrete plans. Martha Ward, MT (ASCP) QIHC Assistant Manager Molecular Diagnostics Lab Dept. of Pathology Wake Forest University Baptist Medical Center Winston-Salem, NC 27157 336-716-2104 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Friday, December 17, 2010 11:10 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Physician Signatures Is everyone concerned about getting a physician's signature on requisitions, or do you have a fool proof plan in place? Inquiring minds want to know... TIA! How many of you have electronic ordering for AP? This from CodeMap Compliance that I get.. Signatures Required on Laboratory Requisitions Although many professional groups and organizations lobbied against the signature requirement, CMS is implementing this provision as part of the CY 2011 MPFS. Starting January 1, 2011, the ordering physician must sign all written or paper requisitions for laboratory tests and services. Clinical laboratories will have to scramble to revise requisitions to include a signature line for the ordering physician. However, those labs that receive test orders via electronic or web-based interfaces should not be affected by the signature requirement included in the 2011 MPFS. In the comments to the proposed 2011 MPFS, CMS states, "Our proposed policy does not concern electronic or telephonic requests, because we do not consider these types of requests to be requisitions. As we discussed previously, a requisition is the actual paperwork, such as a form that is provided to a clinical diagnostic laboratory that identifies the test or tests to be performed for a patient. Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] paperless requisitions
I am posting this question for our AP manager and supervisor. Our anatomic pathology lab is starting to market their services, along with our clinical laboratories. Our LIS would like us to move toward paperless surgical pathology requisitions, to more closely mimic some of the larger outside reference labs. Is anyone out there currently paperless? We would be interested in seeing how you accomplished it, any pitfalls, examples of your on-line req, etc. Thanks in advance for any responses. Martha Ward, MT (ASCP) QIHC Assistant Manager, Molecular Diagnostics Lab Wake Forest University Baptist Medical Center 336-716-2104 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] CAP question ANP.22970
We are doing it by hand as well, into a spread sheet, and then doing the calculations. Martha Ward, MT (ASCP) QIHC Assistant Manager, Molecular Diagnostics Lab Wake Forest University Baptist Medical Center 336-716-2104 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of McMahon, Loralee A Sent: Thursday, October 21, 2010 1:57 PM To: cathy.crump...@tuality.org; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] CAP question ANP.22970 We do this by hand. Usually it involves a summer student to compile all of the data on a spread sheet and a formula to compute the percentage of positive cases. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of cathy.crump...@tuality.org [cathy.crump...@tuality.org] Sent: Thursday, October 21, 2010 1:33 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CAP question ANP.22970 How is everyone handling this new question about Annual Result Co mparison for ER and PR? The question states "...the laboratory at lea= annually compares its patient results with published benchmarks, and eva=ates interobserver variability among the pathologists in the laboratory..= Do you have a separate computer program or do this by hand? <=V> Cathy Crumpton HT(ASCP), Histology Lead Tuality=mmunity Hospital Hillsboro, OR 97123 (503)681-1292 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet