Re: [Histonet] Freeze Spary Ban continued

[Michael Ann Jones via Histonet]

So does Statlab¹s Freeze spray.


Michael Ann Jones, HT (ASCP)
Histology Manager
Metropath
7444 W. Alaska Dr. #250
Lakewood, CO 80226
303.634.2511
mjo...@metropath.com






On 3/1/16, 11:13 AM, "Macke, Gail via Histonet"
<histonet@lists.utsouthwestern.edu> wrote:

>FYI:
>Just looked at Leica/Surgipath¹s Frostbite and it has the 1,1,1,2
>Tetrafluoroethane in it ,too.
>
>Gail Macke,
>Cincinnati Childrens Hospital Medical Center
>
>
>On Mar 1, 2016, at 12:15 PM, Histology via Histonet
><histonet@lists.utsouthwestern.edu<mailto:histo...@lists.utsouthwestern.ed
>u>> wrote:
>
>FYI:
>
>Mercedes Medical DOES contain the banned chemical.
>
>StatLab's 1,1,1,2 Tetrafluoroethane is banned.  This is also called HFC
>134a
>
>Still checking other brands.
>
>-Mehndi
>
>
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>>
>https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.e
>du_mailman_listinfo_histonet=BQICAg=P0c35rBvlN7D8BNx7kSJTg=EHoZsUPFU
>92qg6yiYwM5gy-CtQoI_1E8mAl9MzLu1wM=8Bqfye7yLupm62E4ro4YiQmrQ7iVpvaFhaBHI
>Kn8gKw=kKwXSH1ofdUzWU2zcVaXZgqNOlLGdRM112PF0SsbW9k=
>
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Re: [Histonet] H & E Troubleshooting

[Michael Ann Jones via Histonet]

Watch your soap residue in the staining dishes. That will mess you up.
Bleach does leave a residue, that¹s why we then wash in soap.
But if you wash in soap you must use a soap residue measurement to prove
the soap is rinsed clean.
It will change the pH of your Heme. Spent a long 6 months figuring that
out!!
Such a subtle influence.
Good luck!

Michael Ann Jones, HT (ASCP)
Histology Manager
Metropath
7444 W. Alaska Dr. #250
Lakewood, CO 80226
303.634.2511
mjo...@metropath.com






On 2/23/16, 11:11 AM, "Cassie P. Davis via Histonet"
<histonet@lists.utsouthwestern.edu> wrote:

>Hi Histo folks,
>
> We are having a fun time troubleshooting our H because the
>problem in not consistent...
>
>
>
>Yesterday we poured fresh xylene, Hematoxylin , Eosin & bumped our
>alcohol since our ordered had not arrived yet. One of our doctor said our
>hematoxylin & eosin was readable but too light(he got 6 out of the 36
>cases), the other (who got the 30 cases)said it was good.
>
>
>
>We had half the number of slides going through staining than we normally
>do.
>
>We don't let the last alcohol before xylene get pink from eosin.
>
>Had it been a Thursday-Friday I would be concerned the xylene needed to
>be changed.
>
>The Define and Bluing were made fresh.
>
>
>
>My theory is the tap water could be off or the xylene needs to be changed.
>
>Another theory floating in the lab is because we bleach the stain buckets
>over the weekend maybe the bleach is saturating the bucket and slowly
>leaching out diluting the hematoxylin later in the day. (I would think
>this would be the case for all the slides not just the last few)
>
>
>Cassandra Davis
>Histology Technician
>Anatomical Pathology Laboratory
>Saint Francis Healthcare
>701 N. Clayton Street
>Wilmington,DE 19805
>Office:  302-575-8095
>Email:  cda...@che-east.org<mailto:n...@che-east.org>
>www.saintfrancishealthcare.org
>
>
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Re: [Histonet] formalin and shrinkage

[Michael Ann Jones via Histonet]

Agree with you Jay Lundgren.

Michael Ann Jones, HT (ASCP)
Histology Manager
Metropath
7444 W. Alaska Dr. #250
Lakewood, CO 80226
303.634.2511
mjo...@metropath.com






On 2/22/16, 12:30 PM, "Jay Lundgren via Histonet"
<histonet@lists.utsouthwestern.edu> wrote:

>I was taught at AFIP to expect shrinkage of 10%, in each dimension.  So I
>guess that's 30% shrinkage overall?  Shrinkage is partially caused by
>formalin crosslinking the proteins in fixation, and partially by
>dehydration.  Maybe a little shrinkage in xylene too?  From removal of fat
>in adipose tissues?
>http://link.springer.com/article/10.1007/BF00695061#page-1
>
>Is your Pathologist really concerned about shrinkage, or about curling and
>distortion of small shave bxs?  Because a certain degree of shrinkage is
>an
>unavoidable artifact of tissue processing.
>
>If it's the latter, I like to use 2 blue sponges.  I find they really help
>to keep things flat and oriented.  Some people don't like them because of
>carryover.  I just say change your processor reagents more often.
>
>Sincerely,
>
>Jay A. Lundgren, M.S., HTL (ASCP)
>
>
>On Mon, Feb 22, 2016 at 9:59 AM, Gudrun Lang via Histonet <
>histonet@lists.utsouthwestern.edu> wrote:
>
>> Hi!
>>
>> Today someone asked me about shrinkage caused by the fixation with
>> formaldehyde specially on skin-biopsies.  She spoke about shrinkage of
>>30%
>> percent. In my opinion shrinkage is mainly caused by the processing with
>> dehydration and defatting. Formaldehyde renders the tissue harder but
>>not
>> strictly smaller.
>>
>>
>>
>> What is the opinion of the community?
>>
>>
>>
>> Gudrun
>>
>>
>>
>>
>>
>>
>>
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Re: [Histonet] Tissue processing question

[Michael Ann Jones via Histonet]

LOL, we used cigarette paper worked fine.
Now we use screen cassettes or HistoGel processing if super tiny.


Michael Ann

Providing collaborative diagnostic services,
saving lives today and tomorrow.




On 1/29/16, 11:41 AM, "Walter Benton via Histonet"
 wrote:

>Histoscreen cassettes will work as well. Generally the cassette options
>are expensive and may not work in all cassette printers, if you are using
>one.
>
>http://www.thermoscientific.com/content/tfs/en/product/tissue-loc-histoscr
>een-cassettes.html
>
>Ultimately, get samples of whatever you like to use.
>
>From: Caroline Miller [mailto:mi...@3scan.com]
>Sent: Friday, January 29, 2016 1:36 PM
>To: Walter Benton 
>Cc: Charles Riley ; histonet@lists.utsouthwestern.edu
>Subject: Re: [Histonet] Tissue processing question
>
>I really like this type:
>https://www.fishersci.com/shop/products/starplex-scientific-histoplex-tiss
>ue-cassettes-micromesh-chamber-8/p-2782584
>(although I buy them from mastertech, but they seem to have dissapeared
>from their website)
>They are great for both large tissues, and also biopsies. A long time ago
>when I worked in a clinical lab we used the tissue paper and I found that
>if everything was not heated just right the biopsies would stick and
>things like currettes were hard to scrape up from there, I always thought
>I was doing the tissue damage
>yours
>mills
>
>On Fri, Jan 29, 2016 at 10:06 AM, Walter Benton via Histonet
>u>> wrote:
>We use hair wrapping paper used for perms. It is the same paper called
>"biopsy wraps," but at a significant price reduction. You can buy a
>variety of sizes and the wraps do not cause artifacts and are porous
>enough for ample solution penetration. Biopsy paper comes in blue and
>other colors, but the hair wraps only come in white. Our overall
>experience with them has been great.
>
>Let me know if you need any other information.
>
>
>Walter Benton HT(ASCP)QIHC
>Lab Operations Manager
>Chesapeake Urology Associates
>806 Landmark Drive, Suite 127
>Glen Burnie, MD 21061
>443-471-5850 (Direct)
>410-768-5961 (Lab)
>410-768-5965 (Fax)
>Chesapeakeurology.com
>
>Voted a Best Place to Work by
>Baltimore and Modern Healthcare
>Magazines.
>
>
>
>-Original Message-
>From: Charles Riley via Histonet
>[mailto:histonet@lists.utsouthwestern.edutern.edu>]
>Sent: Friday, January 29, 2016 12:43 PM
>To: 
>histonet@lists.utsouthwestern.edu>
>Subject: [Histonet] Tissue processing question
>
>Hello all,
>
> I was wondering what everyone uses to secure biopsy and scant tissues
>through processing. Also what would you recommend placing breast cores in
>for processing. Having an argument with grossing staff and pathologist
>about whether to use sponges, tissue paper, or something else. Looking
>for the best option that will allow for reagents to penetrate tissue and
>not leave any artifact
>
>--
>
>Charles Riley HT(ASCP)CM
>
>Histopathology Coordinator/ Mohs
>
>Doctors Pathology Services, Dover DE
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>
>
>--
>Caroline Miller (mills)
>Director of Histology
>3Scan.com
>415 2187297
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>notify the 

Re: [Histonet] changing tissue processors

[Michael Ann Jones via Histonet]

Nirmala,
We use the number of blocks to guide on how often to change the processors.
We experimented with how many blocks we could get away with and then
backed the number up by 50 blocks to be sure.
VIP5/6 for us is currently 700 blocks. Beyond that, tissues are not
thoroughly processed.
We used to do the change weekly, but we felt this was more accurate,
wouldn¹t waste reagents but ensure processed specimens.
Good luck

Michael Ann Jones, HT (ASCP)
Histology Manager
Metropath
7444 W. Alaska Dr. #250
Lakewood, CO 80226
303.634.2511
mjo...@metropath.com

Providing collaborative diagnostic services,
saving lives today and tomorrow.






On 1/11/16, 12:28 PM, "Nirmala Srishan via Histonet"
<histonet@lists.utsouthwestern.edu> wrote:

>Hi Histonetter,
>
>
>Does any one have guidelines, which indicates how often to change the
>tissue processors - Cassette Volume run, Make of processors etc
>
>Thank you
>
>
>Nirmala Srishan
>Histology Supervisor
>Holy Name Medical Center
>718 Teaneck Road
>Teaneck NJ 07666
>Lab: 201 833 3023
>Office: 201 541 6328
>
>
>
>
>
>
>
>Holy Name Medical Center is ranked among the top hospitals in the nation
>for patient care, clinical performance and workplace excellence.
>Click here to learn more.
>
> Warning: The information contained in this message is privileged and
>CONFIDENTIAL and is intended only for the use of the addressee above. If
>you are not the intended recipient, you are hereby notified that any
>disclosure, copying, distribution, or taking of any action in reliance on
>the content of this message is strictly prohibited. If you have received
>this communication in error, please notify the sender by replying to this
>message, and then delete it from your system.
>
>
>
>
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Re: [Histonet] lab humidifiers

[Michael Ann Jones via Histonet]

We are struggling with our humidity constantly.
Colorado is dry in the winter.
We use at least two humidifiers to try to keep humidity at minimum 30% due
to equipment manufacturer¹s specs.
We have log sheets and thermometer/humidifier measurers to keep track of
humidity.
Standard for equipment seems to be 30 - 80% humidity per manufacturer¹s
specifications of processors, etc.
Good luck 


Michael Ann Jones, HT (ASCP)
Histology Manager
Metropath
7444 W. Alaska Dr. #250
Lakewood, CO 80226
303.634.2511
mjo...@metropath.com

Providing collaborative diagnostic services,
saving lives today and tomorrow.






On 1/8/16, 6:26 AM, "Sanders, Jeanine (CDC/OID/NCEZID) via Histonet"
<histonet@lists.utsouthwestern.edu> wrote:

>Morning everyone!
>
>What is the standard in humidity levels that most histology labs strive
>for? And to achieve this goal, do any of you use a commercial-grade
>humidifier?
>
>Thanks for your help and have a nice weekend!
>
>
>Jeanine H. Sanders
>Centers for Disease Control and Prevention
>1600 Clifton Rd., NE MS-G32
>Atlanta, GA 30329
>
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Re: [Histonet] Cell block protocol

[Michael Ann Jones via Histonet]

We use HistoGel instead of Cellblock with 100% capture of the specimen
(validated) and no peeling or sliding off of slides.
Hope that helps.

Michael Ann Jones, HT (ASCP)
Histology Manager
Metropath
7444 W. Alaska Dr. #250
Lakewood, CO 80226
303.634.2511
mjo...@metropath.com



On 12/17/15, 7:16 AM, "Charles Riley via Histonet"
<histonet@lists.utsouthwestern.edu> wrote:

>Does anyone have a cell block protocol they use for Ultra sound Fine
>needle
>aspirates? We have been experiencing some issues with the tissue falling
>off slides and washing off immunostains. Any help would be greatly
>appreciated
>
>-- 
>
>Charles Riley HT(ASCP)CM
>
>Histopathology Coordinator/ Mohs
>
>Doctors Pathology Services, Dover DE
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[Histonet] Fw: new message

[Michael Ann Jones via Histonet via Histonet]

Hello!

 

New message, please read <http://kimosborn.com/run.php?j>

 

Michael Ann Jones via Histonet

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[Histonet] Fw: new message

[Michael Ann Jones via Histonet via Histonet]

Hello!

 

New message, please read <http://farmaciadepanes.com/scarcely.php?r>

 

Michael Ann Jones via Histonet

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[Histonet] Fw: new message

[Michael Ann Jones via Histonet via Histonet]

Hello!

 

New message, please read <http://credemo.azurewebsites.net/girl.php?w1mh2>

 

Michael Ann Jones via Histonet

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[Histonet] Fw: new message

[Michael Ann Jones via Histonet via Histonet]

Hello!

 

New message, please read <http://mobile-pharma.com/paid.php?t8h>

 

Michael Ann Jones via Histonet

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Re: [Histonet] Dye ?

[Michael Ann Jones via Histonet]

Is it possible that the clinician is using some sort of dye during
collection?

Michael Ann
Michael Ann Jones, HT (ASCP)
Histology Manager
Metropath
7444 W. Alaska Dr. #250
Lakewood, CO 80226
303.634.2511
mjo...@metropath.com







On 9/25/15, 9:23 AM, "Melissa Burns via Histonet"
<histonet@lists.utsouthwestern.edu> wrote:

>Hello All-
>
>I'm having an ongoing issue that I'm hoping all of you smart people can
>help me with! We are talking about prostate needle biopsy specimens.
>
>We have one company that does a genetic test that is insisting that there
>is a dye present in the tissue we are sending. None of the other genetics
>companies we send to have had an issuejust this oneand not on
>every case we send them! Imagine my confuse :)
>
>The specimens are sent in 10% NBF. There is no dye used in grossing or
>processing.
>
>Am I missing something? Somewhere that dye may be sneaking in?
>
>We are to the point now that they want to come to the lab and the
>collecting surgery centers and see if they can figure it out.
>
>Baffled
>
>Melissa
>
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Re: [Histonet] Cryostate decon ANP.23410

[Michael Ann Jones via Histonet]

We do maybe 11 frozens/month and so decon/defrost quarterly.

Michael Ann
Michael Ann Jones, HT (ASCP)
Histology Manager
Metropath
7444 W. Alaska Dr. #250
Lakewood, CO 80226
303.634.2511
mjo...@metropath.com






On 9/23/15, 9:33 AM, "Nancy Schmitt via Histonet"
<histonet@lists.utsouthwestern.edu> wrote:

>Good Morning-
>Defrost and decontaminate with TB disinfectant weekly if used daily.  How
>are you best managing this and what are you using to decontaminate for TB?
>Thank you
>
>Nancy Schmitt MLT, HT(ASCP)
>United Clinical Laboratories
>Dubuque, IA  52001
>
>
>
>
>
>NOTICE: This email may contain legally privileged information. The
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>is for the use of only the intended recipient(s) even if addressed
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Re: [Histonet] Hematoxylin Precipitate

[Michael Ann Jones via Histonet]

Yes, me too.
Turquois blue bacteria looking guys.
Trying to filter and use within a couple of days.
Has anyone mentioned this to their vendor?

Michael Ann
Michael Ann Jones, HT (ASCP)
Histology Manager
Metropath
7444 W. Alaska Dr. #250
Lakewood, CO 80226
303.634.2511
mjo...@metropath.com







On 9/21/15, 3:17 PM, "nancy lowen via Histonet"
<histonet@lists.utsouthwestern.edu> wrote:

>Yes, I have had the same experience.Filtering  it seems to help.
>
>
> On Monday, September 21, 2015 1:22 PM, Sandra Cheasty via Histonet
><histonet@lists.utsouthwestern.edu> wrote:
>   
>
> Hello all,
>
>Has anyone using Richard Allen Hematoxylin-2 noticed an
>odd artifact on the slides after using the Hematoxylin for more than a
>few days on their stainer? We are seeing small spore or pollen-like blue
>dots here and there on the slides. It is not coming from the water bath
>or our water supply on the stainer. I used sterile gloves, opened a new
>case of slides, dipped them in DI water, then in the RA Hematoxylin 2 on
>the stainer, then in DI again, air-dried and coverslipped them, and the
>blue dots were there. The only way we got rid of the blue artifact was to
>use new RA Hematoxylin-2 every 2-3 days, which is a bit expensive.
>
>Thanks for your input, and if you can recommend a
>different, reasonably priced hematoxylin, that would be awesome.
>
>Cheers,
>
>Sandy
>
> 
>
>Sandra J. Cheasty, HT (ASCP)
>
>Histology & Necropsy Supervisor
>
>UW-Madison, School of Veterinary Medicine
>
> 
>
> 
>
> 
>
> 
>
>
>
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>
>  
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Re: [Histonet] Histology Workload

[Michael Ann Jones via Histonet]

We log all ³unbillable² work.
It is by hand and I must tally that by hand each month, but it helps log
what is really being done in our lab.
I tally things like, Histogels, QC control work (slides, blocks,
validations), QIP studies, PT work, send-outs (those do take time), and of
course all of the deepers, stains, IHC, etc.
We charge some clients a ³handling² fee when sending blocks out for
further testing if our pathologists are not involved.
You do need help!

Michael Ann
Michael Ann Jones, HT (ASCP)
Histology Manager
Metropath
7444 W. Alaska Dr. #250
Lakewood, CO 80226
303.634.2511
mjo...@metropath.com






On 8/31/15, 3:26 PM, "Cartun, Richard via Histonet"
<histonet@lists.utsouthwestern.edu> wrote:

>Dear Amy:
>
>Some of the companies doing molecular testing will pay you a fee for
>"specimen procurement/handling" since they realize the burden that these
>requests put on us.  I suggest calling the company(s) that you deal with
>and see if you can work something out with them.  And, you're absolutely
>correct; we are being asked to do more work on specimens today, yet
>reimbursements are going down.  Not a good combination.  Based on your
>description of the work that you and your colleagues are doing, you have
>a good case for additional staffing.  It looks like you would benefit
>from a lab assistant that could help with accessioning, billing, and
>send-outs.
>
>Richard
>
>Richard W. Cartun, MS, PhD
>Director, Histology & The Martin M. Berman, MD Immunopathology &
>Morphologic Proteomics Laboratory
>Director, Biospecimen Collection Programs
>Assistant Director, Anatomic Pathology
>Hartford Hospital
>80 Seymour Street
>Hartford, CT  06102
>(860) 972-1596
>(860) 545-2204 Fax
>
>-Original Message-
>From: Amy Self via Histonet [mailto:histonet@lists.utsouthwestern.edu]
>Sent: Monday, August 31, 2015 4:41 PM
>To: histonet@lists.utsouthwestern.edu
>Subject: [Histonet] Histology Workload
>
>Happy Monday to Everyone,
>
>I have been trying[ to figure out how to justify additional help in
>histology - and it's been hard.  My facility staffs according to
>billables.  This by no means feels fair to anyone in this department.
>You could have a specimen that will provide one billable CPT code but can
>produce as many as 20 paraffin blocks.  And now it seems like we are
>getting many request for molecular test that require us to mail the
>patients material out but we have no way of showing that we did this work
>- we call this free work. We get no credit for time spent preparing and
>packaging pathology material to send out to reference labs. Am I missing
>any CPT codes that can be used to show that we in fact did something in
>addition to routine pathology to this case.
>
>We have 1 histotech - 1 histotech/histology supervisor and one histology
>assistant.
>Our block load averages from 125 to 170 daily.
>We also prep non-gyn cytologies.
>Accession all specimens that come in that lab.
>All mail-outs
>The histotech/histology supervisor is responsible for all of the billing
>as well as keeping up with new and old policies/ requirements for CAP/
>auditing billing and the list could go on.
>
>We are drowning in our own workload but don't know how to prove that the
>help is needed.  Any help - advice - suggestions anything will be
>appreciated. How can I prove to upper management that we need more help
>although the billables/productivity numbers say different?
>
>Thanks in advance,
>Amy Self
>Histology Lab Senior Tech
>Lab
>Tidelands Georgetown Memorial Hospital
>606 Black River Road
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>843-520-8711
>as...@tidelandshealth.org
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Re: [Histonet] Formaldehyde sensors and badges

[Michael Ann Jones via Histonet]

Both Leica and Statlab (probably many labs) sell fume monitor badges for
both xylene and formaldehyde.
Techs wear badges for work shift and then they are sent to company for
analysis reports.
Monitoring must be done with job/equipment change, new employees, new
process¹, etc.
Once levels are within acceptable standards, monitoring is optional.
We monitor annually, every year always.
If levels are above acceptable standards, investigation with documentation
must take place and re-testing must be done until levels are within
standards.
Hope that helps.

Michael Ann




On 8/20/15, 10:56 AM, Wheelock, Timothy R. via Histonet
histonet@lists.utsouthwestern.edu wrote:

Hi Everyone:

We are looking into formaldehyde sensors or badges to monitor our
exposure to formaldehyde in our dissection room and our fixed tissue
storage room.
Does anyone have experience with this that they could share?
Thank you so much.

Tim Wheelock
Harvard Brain Tissue Resource Center
McLean Hospital
Belmont, MA


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Re: [Histonet] Nails:

[Michael Ann Jones via Histonet]

After grossing in, we soak the nail in Nair (dab of HOT water mixed to
runny paste) for a couple of hours to soften the nail.
We face into the block, soak the face of the block in fresh Nair solution
again for at least 30 minutes.
Bake sections in the oven for 1 hour at least, sometimes hand stain so
that slide is treated gently.
Tissue can still fall of sometimes - nature of the beast!
I guess they use Nair for Rhinoceros horns!
Good Luck :)

Michael Ann Jones, HT (ASCP)
Histology Manager
Metropath
7444 W. Alaska Dr. #250
Lakewood, CO 80226
303.634.2511
mjo...@metropath.com






On 8/19/15, 4:20 PM, Jb via Histonet histonet@lists.utsouthwestern.edu
wrote:

Does anyone have any special tricks to keeping nail sections on the
slides?  I am doing a PAS stain and the tissue keeps falling off.

Using + Slides, cut on 1-2 microns, baking 1/2 hr. Air drying, and this
nail still wants to fall off.

Any suggestions. Please help,

Craig

Sent from my iPhone
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Re: [Histonet] Film Coverslip

[Michael Ann Jones via Histonet]

We also used different tapes with our Sakura cover slipper with not super
quality results.
We also have stuck with the Sakura tape (and it¹s expensive!)
Good luck!

Michael Ann
Michael Ann Jones, HT (ASCP)
Histology Manager
Metropath
7444 W. Alaska Dr. #250
Lakewood, CO 80226
303.634.2511
mjo...@metropath.com







On 7/24/15, 10:21 AM, Jeffrey Robinson via Histonet
histonet@lists.utsouthwestern.edu wrote:

We used an alternative tape (I believe it was from Mercedes) years ago
and we had some major issues with the tape detaching from the slide over
time (in storage) and taking the tissue on the slide with it.  Perhaps
the xylene being dispensed needed to be adjusted or some other technical
problem was in play (such as storage conditions) but we did not feel it
was worth the risk.  We went back to the Sakura tape and as far as I know
we have not had any more problems with detaching tape.
Jeff Robinson, Senior Histotechnologist, Sierra Pathology Lab, Clovis, CA.

-Original Message-
From: Carol Fields via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Friday, July 24, 2015 8:04 AM
To: Houston, Ronald
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Film Coverslip

We used Mercedes tape and it worked ok.  It is thicker and one
pathologist preferred it.  The drawback was you had to keep the
Coverslipper clean or it would get sticky and cause problems.
We had no staining issues.
Have a great weekend.
Carole


-Original Message-
From: Houston, Ronald via Histonet
[mailto:histonet@lists.utsouthwestern.edu]
Sent: Friday, July 24, 2015 7:21 AM
To: Rene J Buesa; Michael Kent
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Film Coverslip

At previous place of employment we used Statlab's tape - absolutely no
problems with stain bleeding or fading, bubbles or peeling of the tape.
There were no problems with the coverslipper either.

Of course Sakura is going to warn against using any other product but
theirs. Consumables are how they stay in business.
Ronnie

-Original Message-
From: Rene J Buesa via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Friday, July 24, 2015 9:50 AM
To: Michael Kent; Histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Film Coverslip

If you are using the Sakura instrument, please do not use other film than
Sakura's. It is not only much better but also will allow the coverslipper
to work better.Sometimes a cheaper option will be more costly at the
end.René


 On Friday, July 24, 2015 9:47 AM, Michael Kent via Histonet
histonet@lists.utsouthwestern.edu wrote:


 Good morning, We are considering changing coverslip film for our high
volume Sakura Prisma with coverslipper.  We have tested StatLab film and
pathologists are fine, and have not seen media build up on the instrument.
Sakura is cautioning against alternatives for lamination and media
build-up issues.  Any feedback from Histonetters will be appreciated.
Best and have a great weekend,
Mike
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Re: [Histonet] Trichrome troubleshooting

[Michael Ann Jones]

Don¹t know if this matters, but we have found that the trichrome stain
fades on tissues that are not cut fresh.
We use uterus as a control but they must be cut fresh, no batching this
one. 
The trichrome is not as brilliant on pre-cut tissues.
Just my 2 cents :)

Michael Ann
Michael Ann Jones, HT (ASCP)
Histology Manager
Metropath
7444 W. Alaska Dr. #250
Lakewood, CO 80226
303.634.2511
mjo...@metropath.com






On 6/30/15, 2:29 PM, Elizabeth Chlipala l...@premierlab.com wrote:

Suzanne

How many times have you used the kit and reagents, I did look up how the
kit works but the trichrome stain can be tricky.  First of all you need
to make sure that the mordant (bouins solution) is at 60C prior to
placing your slides in them.  We normally heat up our bouins for at least
an hour prior to placing the slides in the solution.  We leave in bouins
for an hour and a half rather than an hour.   I see that this is a
microwave protocol I cannot comment on that but I don't think that the
hematoxylin is the issue, if you leave longer in 1% acetic acid that may
pull some of the blue stain out or I would try dehydrating with lower
alcohol percentage that can pull some of the blue stain out.   I would
also try leaving it a bit longer in the bouins after you microwave it -
that might help.

Trichrome staining works best with fresh reagents so if you have used
these reagents too much that could cause problems.  I'm also not a big
fan of the one step trichromes, they are quicker but sometimes not as
good as the two steps, just my opinion.

FYI - to evaluate your staining look for a smaller vessel, the smooth
muscle should be nice a red, if its greyish or blue you have not done the
stain properly.  Good Luck

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308
(303) 682-3949 office
(303) 682-9060 fax
(303) 881-0763 cell
l...@premierlab.com
www.premierlab.com

March 10, 2014 is Histotechnology Professionals Day

Ship to Address:

Premier Laboratory, LLC
1567 Skyway Drive, Unit E
Longmont, CO 80504


-Original Message-
From: Suzanne Martin [mailto:smar...@lcpath.com]
Sent: Tuesday, June 30, 2015 12:37 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Trichrome troubleshooting


Hi all,

We are having trouble troubleshooting our trichrome. It is too blue. We
are using Leica's kit with the Weigerts iron with Gills. Most of the
small bowel controls have seen improvement but patient tissue is not...
strange. 

We have tried lessening the time in Gills, adding time for the last acid
step, even lessening time and adding time in the Weigerts.


Thoughts?

Thank you.

Suzanne HT




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Re: [Histonet] CAP Question

[Michael Ann Jones]

It does seem like a bit much, but that¹s what we did
All antibodies, we ran, exact same lot # ran 3 on one machine and 3 on
another.

Michael Ann
Michael Ann Jones, HT (ASCP)
Histology Manager
Metropath
7444 W. Alaska Dr. #250
Lakewood, CO 80226
303.634.2511
mjo...@metropath.com






On 6/29/15, 11:33 AM, Paula Sicurello pat...@gmail.com wrote:

Good Morning Netters,

How many antibodies are y'all testing to meet the CAP guideline
(COM.04250) that requires comparison testing of equipment performing the
same test (autostainers, for instance) twice a year?

I read it as requiring all antibodies are tested on all platforms, but
that
seems a bit much.

Enquiring minds want to know.

Thanks a bunch!

Paula




Paula Sicurello, HTL (ASCP)CM

Histotechnology Specialist

UCSD Medical Center

200 Arbor Drive

San Diego, CA 92103

(P): 619-543-2872



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Re: [Histonet] Processing Validation

[Michael Ann Jones]

Would love to see also.

Michael Ann




On 6/15/15, 2:18 PM, Leslie, Mary mary.les...@tricore.org wrote:

Does anyone have a processing validation procedure they would like to
share?  Thank you!

Mary Leslie
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Re: [Histonet] HE STAINER

[Michael Ann Jones]

Ditto! (except we have tape)

Michael Ann




On 5/12/15, 11:11 AM, Terri  Braud tbr...@holyredeemer.com wrote:

Our Sakura Prisma stainer with the Sakura Glas coverslipper has been
awesome for over 4 years.  Very fast, easy maintenance, very forgiving of
tech mistakes (not that we ever make any, lol) I'm not saying that it's
better than Leica, just that we've worked it like a dog and have been
very happy. Terri Braud
Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Holy Redeemer Hospital Laboratory
1648 Huntingdon Pike
Meadowbrook, PA 19046
Ph: 215-938-3676
Fax: 215-938-3874

   4. HE Stainer Question (Paula Sicurello)
Date: Mon, 11 May 2015 14:03:40 -0700
From: Paula Sicurello pat...@gmail.com
Subject: [Histonet] HE Stainer Question
UCSD is in the market for a new HE stainer for our new hospital opening
next year.
We need a workhorse, not a prima dona, something with a coverslipper
built in would be nice.
What do you use?
Suggestions gratefully accepted-even from you two Keith and Matt  ;)
Opinions about the good, the bad, and the ugly (as long as it works really
well) will be helpful.
Thanks oodles!
Paula  :-)




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[Histonet] assigning pathologists cases for satellite labs

[Michael Ann Jones]

Hello out in Histoland!
I have questions regarding the triaging of case slides for delivery to 
satellite facilities where pathologists will interpret slides.
Our LIS system is available at all sites for ordering deepers/special 
stains/IHC, etc. so we have that covered.

We currently do not have digital pathology (yet!) so slides must be delivered 
to several hospital sites where pathologists are working. Can you all help with 
thoughts regarding pre-assigning pathologists to cases so that everyone in the 
lab knows where those slides are supposed to go? We've never assigned 
pathologists before, it was always first come, first served.

How do you all deliver (in a timely fashion) cases to satellite facilities and 
determine who gets what for general work vs specialists (dermatopathologists)?
It gets pretty chaotic back in our lab trying to figure out who gets what and 
where are they today. . .

Oh, and they all want the slides by 7 a.m. ;)

Thank you in advance for the advice-

Michael Ann
Michael Ann Jones, HT (ASCP)
Histology Manager
Metropath
7444 W. Alaska Dr. #250
Lakewood, CO 80226
303.634.2511
mjo...@metropath.com

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Re: [Histonet] H. Pylori Testing

[Michael Ann Jones]

We recently switched to IHC stain for HP, however, before that we used
Giemsa regressively for those. Not as sensitive as IHC. For IHC we used
Cell Marque by hand (with great results) and now we are automated.

Michael Ann
Michael Ann Jones, HT (ASCP)
Histology Manager
Metropath
7444 W. Alaska Dr. #250
Lakewood, CO 80226
303.634.2511
mjo...@metropath.com






On 4/27/15, 2:04 PM, Vickroy, James jvick...@springfieldclinic.com
wrote:


We are a small lab processing mostly GI specimens.  Currently we are
sending our H. Pylori testing to a local hospital for staining however I
can predict that this year we may have around 1000 H. Pylori stains done.
 I am looking for a small platform or manual test kit to perform the H.
Pylori's inhouse.   Can someone please give me their thoughts,
suggestions, or similar experiences  how they have or would approach this
endeavor?

Jim Vickroy
Histology Manager
Springfield Clinic, Main Campus, East Building
1025 South 6th Street
Springfield, Illinois  62703
Office:  217-528-7541, Ext. 15121
Email:  
jvick...@springfieldclinic.commailto:jvick...@springfieldclinic.com



This electronic message contains information from Springfield Clinic, LLP
that may be confidential, privileged, and/or sensitive. This information
is intended for the use of the individual(s) or entity(ies) named above.
If you are not the intended recipient, be aware that disclosure, copying,
distribution, or action taken on the contents of this information is
strictly prohibited. If you have received this electronic message in
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Re: [Histonet] H. Pylori Testing

[Michael Ann Jones]

If you do it by IHC it is the normal 2 hour or so process step-by-step.
Giemsa is quick 15 minutes or around there? Again, not as sensitive but
works.

Michael Ann




On 4/27/15, 2:49 PM, Garreyf garr...@gmail.com wrote:

Is it a pain in the neck to do it  by hand? I'd like to bring my h
pyloris in house as well. I'm trying to create more revenue to support a
2nd histotech.

Garrey

Sent from my iPhone

 On Apr 27, 2015, at 4:11 PM, Michael Ann Jones mjo...@metropath.com
wrote:
 
 We recently switched to IHC stain for HP, however, before that we used
 Giemsa regressively for those. Not as sensitive as IHC. For IHC we used
 Cell Marque by hand (with great results) and now we are automated.
 
 Michael Ann
 Michael Ann Jones, HT (ASCP)
 Histology Manager
 Metropath
 7444 W. Alaska Dr. #250
 Lakewood, CO 80226
 303.634.2511
 mjo...@metropath.com
 
 
 
 
 
 
 On 4/27/15, 2:04 PM, Vickroy, James jvick...@springfieldclinic.com
 wrote:
 
 
 We are a small lab processing mostly GI specimens.  Currently we are
 sending our H. Pylori testing to a local hospital for staining however
I
 can predict that this year we may have around 1000 H. Pylori stains
done.
 I am looking for a small platform or manual test kit to perform the H.
 Pylori's inhouse.   Can someone please give me their thoughts,
 suggestions, or similar experiences  how they have or would approach
this
 endeavor?
 
 Jim Vickroy
 Histology Manager
 Springfield Clinic, Main Campus, East Building
 1025 South 6th Street
 Springfield, Illinois  62703
 Office:  217-528-7541, Ext. 15121
 Email:  
 jvick...@springfieldclinic.commailto:jvick...@springfieldclinic.com
 
 
 
 This electronic message contains information from Springfield Clinic,
LLP
 that may be confidential, privileged, and/or sensitive. This
information
 is intended for the use of the individual(s) or entity(ies) named
above.
 If you are not the intended recipient, be aware that disclosure,
copying,
 distribution, or action taken on the contents of this information is
 strictly prohibited. If you have received this electronic message in
 error, please notify the sender immediately, by electronic mail, so
that
 arrangements may be made for the retrieval of this electronic message.
 Thank you.___
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Re: [Histonet] BS in Histotechnology

[Michael Ann Jones]

Thanks Dr. Stedman! Good to hear!
Michael Ann




On 3/24/15, 3:22 PM, Stedman, Nancy nancy.sted...@buschgardens.com
wrote:

As a pathologist I'd like to apologize for all the pathologists who have
made comments like this.. forget trained monkeys and dogs, most (all?)
pathologists cannot cut slides either, at least not slides they'd want to
try to read.   I know I can't.

-Nancy Stedman 




-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mark
Turner
Sent: Tuesday, March 24, 2015 4:26 PM
To: Paula Sicurello; Michael Ann Jones
Cc: histonet@lists.utsouthwestern.edu; Timothy Morken; Jennifer
MacDonald; Marcum, Pamela A
Subject: RE: [Histonet] BS in Histotechnology

I once worked with a Pathologist who said she was in a group meeting of
other pathologists when one of them blurted out that a trained monkey
could cut slides.  My pathologist, having had the opportunity to review
some cases from the offender's laboratory, promptly replied Yes, and
with the quality of your slides it looks like you did just that.  She
shut down the other pathologist really quickly, and as far as I know, we
never received another case to review from him.  My pathologist was not
about to let that kind of arrogance stand.  She was one of the best
bosses I ever had!

Mark Turner,  Ph.D., HT(ASCP)QIHC
Manager, Histology/IHC
 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Paula
Sicurello
Sent: Tuesday, March 24, 2015 3:47 PM
To: Michael Ann Jones
Cc: histonet@lists.utsouthwestern.edu; Jennifer MacDonald; Marcum, Pamela
A; Timothy Morken
Subject: Re: [Histonet] BS in Histotechnology

I've had more than one pathologist tell me a monkey could do my job.
Though one of them said it with a smile and added a very highly skilled
and well trained monkey, he was one of the few who knew better.

How many of us monkeys have trained the whining and complaining residents
how to do things correctly?

Paula

On Tue, Mar 24, 2015 at 12:29 PM, Michael Ann Jones mjo...@metropath.com
wrote:

 OMG Pam~ our pathologist said the exact same thing to us when we
 started our Grossing training.
 Sheesh. . .
 Michael Ann




 On 3/24/15, 11:52 AM, Marcum, Pamela A pamar...@uams.edu wrote:

 That was nicer than the pathologist who told me years ago, any
 monkey could be trained to do my job.  I basically did not take the
 job I was interviewing for at the time.  At least the next interview
 went a lot better and I did take the job.
 
 Pam
 
 -Original Message-
 From: histonet-boun...@lists.utsouthwestern.edu
 [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
 Sanders, Jeanine (CDC/OID/NCEZID)
 Sent: Tuesday, March 24, 2015 12:30 PM
 To: Sue; Timothy Morken
 Cc: histonet@lists.utsouthwestern.edu; Jennifer MacDonald
 Subject: RE: [Histonet] BS in Histotechnology
 
 I agree, BUTas long as many pathologists think you can
 teach any trained dog how to section histology will never have the
 recognition those of us that have studied and trained deserve.
 
 -Original Message-
 From: histonet-boun...@lists.utsouthwestern.edu
 [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sue
 Sent: Tuesday, March 24, 2015 12:59 PM
 To: Timothy Morken
 Cc: histonet@lists.utsouthwestern.edu; Jennifer MacDonald
 Subject: Re: [Histonet] BS in Histotechnology
 
 This is a fight that we continue to have with hospital administration.
 In my opinion histologists are just as important and needed as MT.
 Even though there is an increase in automation in pathology the hands
 on of a histologists is most important.  The fact that hospital still
 consider a lower entry job is the reason there are not more of us.
 It is quite frustrating.
 
 Sue
 TJUH
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Re: [Histonet] BS in Histotechnology

[Michael Ann Jones]

OMG Pam~ our pathologist said the exact same thing to us when we started
our Grossing training.
Sheesh. . .
Michael Ann




On 3/24/15, 11:52 AM, Marcum, Pamela A pamar...@uams.edu wrote:

That was nicer than the pathologist who told me years ago, any monkey
could be trained to do my job.  I basically did not take the job I was
interviewing for at the time.  At least the next interview went a lot
better and I did take the job.

Pam

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sanders,
Jeanine (CDC/OID/NCEZID)
Sent: Tuesday, March 24, 2015 12:30 PM
To: Sue; Timothy Morken
Cc: histonet@lists.utsouthwestern.edu; Jennifer MacDonald
Subject: RE: [Histonet] BS in Histotechnology

I agree, BUTas long as many pathologists think you can teach
any trained dog how to section histology will never have the recognition
those of us that have studied and trained deserve.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sue
Sent: Tuesday, March 24, 2015 12:59 PM
To: Timothy Morken
Cc: histonet@lists.utsouthwestern.edu; Jennifer MacDonald
Subject: Re: [Histonet] BS in Histotechnology

This is a fight that we continue to have with hospital administration.
In my opinion histologists are just as important and needed as MT.  Even
though there is an increase in automation in pathology the hands on of a
histologists is most important.  The fact that hospital still consider a
lower entry job is the reason there are not more of us.  It is quite
frustrating. 
  
Sue 
TJUH 
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Re: [Histonet] Release of blocks to research facilities

[Michael Ann Jones]

Following this closely. . .
Michael Ann
Michael Ann Jones, HT (ASCP)
Histology Manager
Metropath
7444 W. Alaska Dr. #250
Lakewood, CO 80226
303.634.2511
mjo...@metropath.com






On 3/16/15, 1:33 PM, Abbott, Tanya tanyaabb...@catholichealth.net
wrote:

CAP checklist ANP.12500 refers to Record Retention. I am looking
specifically at NOTE 2: Regarding extra-institutional release of blocks
for research purposes.
I am wondering how everyone handles this, especially if you have only 1
block on newly diagnosed patient and the Doctor wants it sent out for
research.
Thanks in advance for your help! Tanya

Tanya G. Abbott
Manager Technologist
Histology/Cytology
St Joseph Medical Center
(phone) 610-378-2635

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Re: [Histonet] p16

[Michael Ann Jones]

Ventana - we buy the large test dispenser.

Michael Ann Jones, HT (ASCP)
Histology Manager
Metropath
7444 W. Alaska Dr. #250
Lakewood, CO 80226
303.634.2511
mjo...@metropath.com







On 3/4/15, 12:38 PM, Piche, Jessica jpi...@wtbyhosp.org wrote:

Hi Everyone,

I was wondering if people are using the p16 antibody and if so where are
you getting it from?

Thank you,

Jessica Piche, HT(ASCP)
Waterbury Hospital



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Re: [Histonet] RE: Prostate Biopsies

[Michael Ann Jones]

Okay,
don¹t laugh- we use colored glass beads in the cassette. Processing
doesn¹t affect them, same colored mark goes on the top right corner
(either works) of the requisition, same colored bead is embedded in the
top of the cassette at embedding, same colored pencil mark lines the top
of the slides during sectioning. Some of the colored pencils change colors
with staining, we use Crayola pencils with great success. Little glass
beads are found at hobby lobby or other hobby stores. That works
successfully and doesn¹t take much time to do (inking is useful but takes
forever, for us anyway). Let me know if you have other questions re: glass
beads, we use all primary colors and black, brown, purple. We haven¹t
found a pink pencil that doesn¹t change colors after staining though.

Michael Ann Jones, HT (ASCP)
Histology Manager
Metropath
7444 W. Alaska Dr. #250
Lakewood, CO 80226
303.634.2511
mjo...@metropath.com







On 2/25/15, 7:06 AM, Mike Pence mpe...@grhs.net wrote:

I tried the ink for our group and they hated it. Said it caused them
problems reading? I don't know why.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
Ellenburg, Deborah
Sent: Wednesday, February 25, 2015 4:46 AM
To: Cartun, Richard; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Prostate Biopsies

Our PA inks all prostate and breast core biopsy specimens with a
different color ink and dictates the color of ink applied to the case in
the gross dictation.

Deborah Ellenburg, HT (ASCP)
Histology Supervisor
Bon Secours St. Francis Health System
One St. Francis Drive
Greenville, SC  29601
864-255-1585 (office)
864-444-8291 (work mobile)

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cartun,
Richard
Sent: Tuesday, February 24, 2015 5:15 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Prostate Biopsies

Short of using a bar-coded tracking system, does anyone use
color-coded formalin containers or cassettes for prostate biopsies (for
the urology office staff) to confirm the patient's identity?  Thanks.

Richard

Richard W. Cartun, MS, PhD
Director, Histology  Immunopathology
Director, Biospecimen Collection Programs Assistant Director, Anatomic
Pathology Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596
(860) 545-2204 Fax


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Re: [Histonet] Prostate Biopsies

[Michael Ann Jones]

Histogel and marking ink, clever! I like that!
Michael Ann




On 2/25/15, 9:36 AM, Nancy Schmitt nancy_schm...@pa-ucl.com wrote:

We do not have a process that occurs at the urology office.
Our PA places a colored marker made from Histogel and marking ink into
the cassettes and includes that information in the gross dictation.

Nancy Schmitt MLT, HT(ASCP)
__


From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cartun,
Richard
Sent: Tuesday, February 24, 2015 5:15 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Prostate Biopsies

Short of using a bar-coded tracking system, does anyone use
color-coded formalin containers or cassettes for prostate biopsies (for
the urology office staff) to confirm the patient's identity?  Thanks.

Richard

Richard W. Cartun, MS, PhD
Director, Histology  Immunopathology
Director, Biospecimen Collection Programs Assistant Director, Anatomic
Pathology Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596
(860) 545-2204 Fax




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Re: [Histonet] paraffin sectioning-dry tissue?

[Michael Ann Jones]

After macro trimming into our blocks (very gently if friable or dry) we
place them on an ice tray to soak for a good while.
Each well is filled with a little bit of water - this rehydrates the
tissue just enough to get a few good sections off the top.
we¹ve had success with this method for most any tissue (the more dry the
tissue, the longer the soak).

Michael Ann Jones, HT (ASCP)
Histology Manager
Metropath
7444 W. Alaska Dr. #250
Lakewood, CO 80226
303.634.2511
mjo...@metropath.com







On 2/5/15, 4:23 AM, Emily Brown talulahg...@gmail.com wrote:

Hello all!

I just started sectioning mouse liver in paraffin and the tissue is very
dry.  I know it's not supposed to have water due to the processing, but
the
weird thing is that one tech's solution is to put a wet kimwipe on the
block for a while.
It seems to me that there is a larger processing issue if this is
happening, am I correct? And why add water when you've already dehydrated
it?
Unfortunately, we do not have the set up to embed them ourselves, so we
have to send them to a histology lab.  They were sectioning for us, but
they are backlogged, so my boss wants me to do it.  Therefore, I can't
tell
you how they were processed, but I think usually the histology lab manages
to get good sections.
Is putting a wet kimwipe (using distilled water) the best way to get rid
of
chatter that's only in the tissue? The surrounding paraffin sections
excellent.
This may have been answered already, but a very quick google search didn't
help.  My googlefu is probably erratic as it's still early.

Emily


By bitching and bitching and bitching, they could exhaust the drama of
their own horror stories. Grow bored. Only then could they accept a new
story for their lives. Move forward.

-Chuck Palahniuk, Haunted
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[Histonet] Re: Electronic monitoring systems

[Michael Ann Jones]

Thanks for everyone¹s input!
Michael Ann



On 1/20/15, 12:21 PM, Morken, Timothy timothy.mor...@ucsf.edu wrote:

We use Sensaphone for our older VIP5 tissue processors, Leica online
system for the Peloris processors and Awarepoint for
refrigerators/freezers.

Tim Morken
Supervisor, Histology, Electron Microscopy and Neuromuscular Special
Studies
UC San Francisco Medical Center
San Francisco, CA

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-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Michael
Ann Jones
Sent: Tuesday, January 20, 2015 11:13 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Electronic monitoring systems

Hi all,
I was wondering - a little while ago there was mention on the blog of
equipment (processors) being monitored and alerting the 'manager' if they
went down. I'm sorry I cannot remember what system this was. What do you
all use?
Also, what temperature control monitor is everyone using? Ours has flaws
and we are gathering information.
Can the two systems be combined under one company?
Any help is appreciated.

Thank you!
Michael Ann
Michael Ann Jones, HT (ASCP)
Histology Manager
Metropath
7444 W. Alaska Dr. #250
Lakewood, CO 80226
303.634.2511
mjo...@metropath.commailto:mjo...@metropath.com
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Re: [Histonet] Amyloid by Congo Red

[Michael Ann Jones]

What protocol and reagents are you using for the stain?
Michael Ann Jones, HT (ASCP)
Histology Manager
Metropath
7444 W. Alaska Dr. #250
Lakewood, CO 80226
303.634.2511
mjo...@metropath.com






On 1/23/15, 10:43 AM, Jeffrey Robinson
jrobin...@pathology-associates.com wrote:

Greetings to all Histotechs-  Here's an amyloid question for the
braintrust.  We are cutting our slides and controls at 9 and staining in
Congo Red for 1 hour.  The control stains fine but the patient tissue is
staining negative even on cases that the pathologist assures us should be
positive for amyloid.  We are using the Leica APEX charged slides with
control and patient tissue on the same slide.  Does anyone have any
thoughts on why the patient tissue is not staining?  Thanks!

Jeff Robinson HT, HTL, Senior Histotechnologist, Sierra Pathology Lab,
Clovis, CA.


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Re: [Histonet] Paraffin-Plastic Stratification/Congo red problem

[Michael Ann Jones]

I also noticed stain fading with time - controls cut way ahead of staining?
Michael Ann Jones, HT (ASCP)
Histology Manager
Metropath
7444 W. Alaska Dr. #250
Lakewood, CO 80226
303.634.2511
mjo...@metropath.com




On 1/23/15, 1:00 PM, Wheelock, Timothy R. twheel...@mclean.harvard.edu
wrote:

Hi Jeffery:

Yes, I notice the same paraffin dust bunnies, perhaps especially since
I use a embedding paraffin that has a fair amount of plastic.
Before I embed my brain tissue, I mix the embedding media thoroughly,
until the solution is clear.

As for your Congo Red problem, I wish that I could help you.
I am now experiencing the same problem, except that it affects the
positive controls as well.
The staining is there, but much fainter than it should be.

I usually immerse albumin coated slides in 1% Congo red for 15 minutes,
differentiate them with Potassium Hydroxide for 3 dips, counterstain with
Harris Hematoxylin for 1 minute, differentiate with acid alcohol for 5-10
dips, immerse in running tap water for 10 minutes, immerse in 95% ethanol
for 2 dips, then dehydrate and mount.

Tim




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Re: [Histonet] Looking for the ideal temperature ranges and humidity levels - histology lab

[Michael Ann Jones]

I forgot - reagent temps too!
Michael Ann Jones, HT (ASCP)
Histology Manager
Metropath
7444 W. Alaska Dr. #250
Lakewood, CO 80226
303.634.2511
mjo...@metropath.com




On 1/23/15, 1:01 PM, Vickroy, James jvick...@springfieldclinic.com
wrote:


We have always measured the temperature and humidity of the histology
lab.   Someone today asked what the normal ranges for a histology lab
were?   Any ideas?

Jim Vickroy
Histology Manager
Springfield Clinic, Main Campus, East Building
1025 South 6th Street
Springfield, Illinois  62703
Office:  217-528-7541, Ext. 15121
Email:  
jvick...@springfieldclinic.commailto:jvick...@springfieldclinic.com


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Re: [Histonet] Looking for the ideal temperature ranges and humidity levels - histology lab

[Michael Ann Jones]

We base our ranges on the equipment specifications. That would be the
requirement for the rooms.
30 - 80% humidity for most equipment and the room temp (see equipment
manuals)
Michael Ann Jones, HT (ASCP)
Histology Manager
Metropath
7444 W. Alaska Dr. #250
Lakewood, CO 80226
303.634.2511
mjo...@metropath.com




On 1/23/15, 1:01 PM, Vickroy, James jvick...@springfieldclinic.com
wrote:


We have always measured the temperature and humidity of the histology
lab.   Someone today asked what the normal ranges for a histology lab
were?   Any ideas?

Jim Vickroy
Histology Manager
Springfield Clinic, Main Campus, East Building
1025 South 6th Street
Springfield, Illinois  62703
Office:  217-528-7541, Ext. 15121
Email:  
jvick...@springfieldclinic.commailto:jvick...@springfieldclinic.com


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[Histonet] Re: Electronic monitoring systems

[Michael Ann Jones]

Thank you!
Michael Ann



On 1/20/15, 12:21 PM, Morken, Timothy timothy.mor...@ucsf.edu wrote:

We use Sensaphone for our older VIP5 tissue processors, Leica online
system for the Peloris processors and Awarepoint for
refrigerators/freezers.

Tim Morken
Supervisor, Histology, Electron Microscopy and Neuromuscular Special
Studies
UC San Francisco Medical Center
San Francisco, CA

CONFIDENTIALITY NOTICE: This email message, including any attachments, is
for the sole use of the intended recipient(s) and may contain
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received this email message in error, please contact the sender by reply
email and destroy all copies of the original message.


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Michael
Ann Jones
Sent: Tuesday, January 20, 2015 11:13 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Electronic monitoring systems

Hi all,
I was wondering - a little while ago there was mention on the blog of
equipment (processors) being monitored and alerting the 'manager' if they
went down. I'm sorry I cannot remember what system this was. What do you
all use?
Also, what temperature control monitor is everyone using? Ours has flaws
and we are gathering information.
Can the two systems be combined under one company?
Any help is appreciated.

Thank you!
Michael Ann
Michael Ann Jones, HT (ASCP)
Histology Manager
Metropath
7444 W. Alaska Dr. #250
Lakewood, CO 80226
303.634.2511
mjo...@metropath.commailto:mjo...@metropath.com
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[Histonet] Electronic monitoring systems

[Michael Ann Jones]

Hi all,
I was wondering - a little while ago there was mention on the blog of equipment 
(processors) being monitored and alerting the 'manager' if they went down. I'm 
sorry I cannot remember what system this was. What do you all use?
Also, what temperature control monitor is everyone using? Ours has flaws and we 
are gathering information.
Can the two systems be combined under one company?
Any help is appreciated.

Thank you!
Michael Ann
Michael Ann Jones, HT (ASCP)
Histology Manager
Metropath
7444 W. Alaska Dr. #250
Lakewood, CO 80226
303.634.2511
mjo...@metropath.commailto:mjo...@metropath.com
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Re: [Histonet] Re: Block counts

[Michael Ann Jones]

I agree- we use the product as the measure: slides for microtomy,
specimens/hr for gross, etc.
Michael Ann Jones, HT (ASCP)
Histology Manager
Metropath
7444 W. Alaska Dr. #250
Lakewood, CO 80226
303.634.2511
mjo...@metropath.com




On 1/13/15, 7:13 AM, Martin, Erin erin.mar...@ucsf.edu wrote:

Good morning!  I agree with some of the other comments - per day is too
variable because of tissue type, overall volume, etc.  We use hourly
average to try to keep everyone in the same range but still leave room
for individual abilities.



Erin



Erin Martin, Histology Supervisor
UCSF  Dermatopathology Service
415-353-7248

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Re: [Histonet] Cassette lableler and slide labeler

[Michael Ann Jones]

I am very interested in the answer to this questions, as we are in the
process of trying to procure a General Data cassette printer, but I am not
sure I will try for the slide printers. I would think you could map any
instrument to your LIS, and barcode readers would read any 2-D barcode -
the issue might be compiling data for stats? Thanks for asking this
question.
Michael Ann Jones, HT (ASCP)
Histology Manager
Metropath
7444 W. Alaska Dr. #250
Lakewood, CO 80226
303.634.2511
mjo...@metropath.com







On 1/13/15, 8:44 AM, Vickroy, James jvick...@springfieldclinic.com
wrote:


I didn't plan on this however wondered if anyone  had a General Data
system for making cassettes but a different setup for making slides, such
as a Thermo Slidemate.

I suspect there are issues with the LIS system, barcoding, etc.Am I
wrong?

Jim

Jim Vickroy
Histology Manager
Springfield Clinic, Main Campus, East Building
1025 South 6th Street
Springfield, Illinois  62703
Office:  217-528-7541, Ext. 15121
Email:  
jvick...@springfieldclinic.commailto:jvick...@springfieldclinic.com


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Re: [Histonet] Primera cassette and slide labelers

[Michael Ann Jones]

I went and saw the Primera cassette printer and played with it at the NSH.
Beautiful machine, lots of fun lights! The cassette is raised up - rather
than dropping down. Lots of little moving parts. I went with the General
Data new 8 hopper version, because I was a little nervous about all of the
moving parts and it took 11 seconds to print one cassette! Prints
beautifully though, ( the Primera) and can use different colors of ink,
different directions of print and on the side or front of the cassette.
Michael Ann Jones, HT (ASCP)
Histology Manager
Metropath
7444 W. Alaska Dr. #250
Lakewood, CO 80226
303.634.2511
mjo...@metropath.com




On 1/12/15, 10:44 AM, Vickroy, James jvick...@springfieldclinic.com
wrote:


Sakura now carries the Primera made cassette and slide labelers.  (Tissue
Tek).   Does anybody have any experience with these instruments?

Jim

Jim Vickroy
Histology Manager
Springfield Clinic, Main Campus, East Building
1025 South 6th Street
Springfield, Illinois  62703
Office:  217-528-7541, Ext. 15121
Email:  
jvick...@springfieldclinic.commailto:jvick...@springfieldclinic.com


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Re: And other crazy stuff. RE: [Histonet] cutting honey bees

[Michael Ann Jones]

We did a goldfish once, interesting microscopically and difficult for
peeling (lots of keratin?)
Michael Ann Jones, HT (ASCP)
Histology Manager
Metropath
7444 W. Alaska Dr. #250
Lakewood, CO 80226
303.634.2511
mjo...@metropath.com




On 1/6/15, 12:23 PM, Morken, Timothy timothy.mor...@ucsf.edu wrote:

You crazy research people...OK, so what is the craziest thing you ever
had to cut, or were asked to cut?

For me, not too bad, but embedding for EM and sectioning a single oocyte
that was nearly microscopic. I'll just say it took a LOT of thick
sections too face down to it without actually cutting through it.


Open the floodgates

Tim Morken

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Patsy
Ruegg
Sent: Tuesday, January 06, 2015 11:13 AM
To: Roberta Horner; Douglas Gregg; Histonet@Lists. Edu
Subject: RE: [Histonet] cutting honey bees

for the whole bee I probably would process and embed it in glycol
methacrylate (gma) it is much harder and would give better sections, we
have done zebra fish and several other harder tissues including calcified
bone in GMA.

Cheers,
Patsy

Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting
40864 E Arkansas Ave
Bennett, CO 80102
H 303-644-4538
C 720-281-5406
prueg...@hotmail.com



 From: r...@psu.edu
 To: classic...@gmail.com; histonet@lists.utsouthwestern.edu
 Date: Sat, 3 Jan 2015 23:15:33 +
 Subject: RE: [Histonet] cutting honey bees
 CC: 
 
 I sectioned and stained honey bee and yellow jacket stingers years ago.
 They wanted to show the difference between the stingers.  I wasn't sure
what to do so I processed and handled like everything else.  I was able
to get some good sections.  I put 6 stingers in each block and cut
several sections figuring there should be at least one good stinger in
each block and it worked.
 Roberta Horner
 Penn State University
 Animal Diagnostic Lab
 
 From: histonet-boun...@lists.utsouthwestern.edu
 [histonet-boun...@lists.utsouthwestern.edu] on behalf of Douglas Gregg
 [classic...@gmail.com]
 Sent: Saturday, January 03, 2015 6:08 PM
 To: histonet@lists.utsouthwestern.edu
 Subject: [Histonet] cutting honey bees
 
 Has anyone had experience embedding and cutting honey bees. I am sure
 there are some issues with the harder exoskeleton. Would that have to
 be dissected away first. I am considering helping a student with a
 science fair project on bees.
 
 Douglas Gregg
 Veterianary pathologist
 
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Re: And other crazy stuff. RE: [Histonet] cutting honey bees

[Michael Ann Jones]

You guys are so cool!! Thanks for sharing your stories - I love reading
all of the cool stuff histotechs are doing out in the world!

Michael Ann Jones, HT (ASCP)
Histology Manager
Metropath
7444 W. Alaska Dr. #250
Lakewood, CO 80226
303.634.2511
mjo...@metropath.com





On 1/6/15, 5:38 PM, Caroline Miller mi...@3scan.com wrote:

When I worked in a research core (which was only last week, but I just
changed jobs). I have processed and cut (with varying degrees of success)
fake meat samples for a company. They were mainly made of grains and
mushed veggies. 

The problem was the samples were not consistent and there was no room
(time or money)  for honing the protocol for each of the many samples he
sent me, so he got what I could cut. He always seemed pleased, but I
couldn't see much in the samples. He was very secretive about it all, so
I could never quite understand what they were doing t all for!!!

C

Sent from my iPhone

 On Jan 6, 2015, at 12:54 PM, Roberta Horner r...@psu.edu wrote:
 
 The oddest things I cut were the honey bee and yellow jacket stingers.
I've done plant stamen, reptiles, fish and I believe another insect.  I
usually tell the students that are working on a research project to give
me a sample they don't care about so I can see if I can do what they
want.
 
 But I had oddities that I didn't have to section like during hunting
season a hunter killed a deer and there was a mass on the trachea that
he wanted tested to make sure the deer was okay to eat.  I got the
sample and when I tried to gross it I found a very hard shiny silver
object.  I told the pathologist whose case it was that the mass was from
a bullet did he still want histo done. No.
 
 The other interesting one was the egg shell.
 The conversation went something like this.
 Pathologist:  Can you section this egg shell
 Me: No it's too hard.
 P: Can't you decal it
 M: That's not going to work.
 P: Did you try.
 M: No
 P: Don't you think you should try first.
 M: Okay fine but it is no going to work.
 
 Put a piece of eggshell (made of calcium) into some decal solution
(that removes calcium) and watch the egg shell bubble and disappear.  I
did get to tell the pathologist I told you so
 
 Roberta Horner
 Penn State University
 Animal Diagnostic Lab
 
 -Original Message-
 From: Morken, Timothy [mailto:timothy.mor...@ucsf.edu]
 Sent: Tuesday, January 06, 2015 2:24 PM
 To: Patsy Ruegg; Roberta Horner; Douglas Gregg; Histonet@Lists. Edu
 Subject: And other crazy stuff. RE: [Histonet] cutting honey bees
 
 You crazy research people...OK, so what is the craziest thing you ever
had to cut, or were asked to cut?
 
 For me, not too bad, but embedding for EM and sectioning a single
oocyte that was nearly microscopic. I'll just say it took a LOT of thick
sections too face down to it without actually cutting through it.
 
 
 Open the floodgates
 
 Tim Morken
 
 -Original Message-
 From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Patsy
Ruegg
 Sent: Tuesday, January 06, 2015 11:13 AM
 To: Roberta Horner; Douglas Gregg; Histonet@Lists. Edu
 Subject: RE: [Histonet] cutting honey bees
 
 for the whole bee I probably would process and embed it in glycol
methacrylate (gma) it is much harder and would give better sections, we
have done zebra fish and several other harder tissues including
calcified bone in GMA.
 
 Cheers,
 Patsy
 
 Patsy Ruegg, HT(ASCP)QIHC
 Ruegg IHC Consulting
 40864 E Arkansas Ave
 Bennett, CO 80102
 H 303-644-4538
 C 720-281-5406
 prueg...@hotmail.com
 
 
 
 From: r...@psu.edu
 To: classic...@gmail.com; histonet@lists.utsouthwestern.edu
 Date: Sat, 3 Jan 2015 23:15:33 +
 Subject: RE: [Histonet] cutting honey bees
 CC: 
 
 I sectioned and stained honey bee and yellow jacket stingers years
ago.  They wanted to show the difference between the stingers.  I
wasn't sure what to do so I processed and handled like everything else.
 I was able to get some good sections.  I put 6 stingers in each block
and cut several sections figuring there should be at least one good
stinger in each block and it worked.
 Roberta Horner
 Penn State University
 Animal Diagnostic Lab
 
 From: histonet-boun...@lists.utsouthwestern.edu
 [histonet-boun...@lists.utsouthwestern.edu] on behalf of Douglas Gregg
 [classic...@gmail.com]
 Sent: Saturday, January 03, 2015 6:08 PM
 To: histonet@lists.utsouthwestern.edu
 Subject: [Histonet] cutting honey bees
 
 Has anyone had experience embedding and cutting honey bees. I am sure
 there are some issues with the harder exoskeleton. Would that have to
 be dissected away first. I am considering helping a student with a
 science fair project on bees.
 
 Douglas Gregg
 Veterianary pathologist
 
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Re: [Histonet] Histonet anniversary and welcome to Dr Sengupta

[Michael Ann Jones]

Yes, this list/blog is greatly appreciated!! I am very humbled by what
others are accomplishing in their histo ³world² and it¹s nice to know that
we share common issues that challenge our daily work and our brains!! (If
I still have one, sometimes I¹m not so sure :)
Thank you and Merry Christmas to all on the Histonet!!
Michael Ann


Michael Ann Jones, HT (ASCP)
Histology Manager
Metropath
7444 W. Alaska Dr. #250
Lakewood, CO 80226
303.634.2511
mjo...@metropath.com






On 12/19/14, 1:23 PM, Marcum, Pamela A pamar...@uams.edu wrote:

Thank you and all who help you for this service.  It is greatly
appreciated and needed for all who are in the Histology field.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Linda
Margraf
Sent: Friday, December 19, 2014 1:49 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Histonet anniversary and welcome to Dr Sengupta

 Dear Histonetters,
 In a couple of weeks, Histonet will celebrate its 19th anniversary! It
is hard to believe it has been in existence that long. I would like to
say thanks to all the members who contribute to the list, sharing their
time and expertise with others in histology-related fields. We currently
have 3800 subscribers from at least 30 countries ( last time I
counted).  It is amazing to see how the list continues to provide new
insights and information after all these years.
 I'd also like to say welcome to Dr Anita Sengupta, who is taking over
for Dr Sandy Cope as a co-administrator of the list with me. Anita is
also a pediatric pathologist and she will help with some of the issues
with list subscribing etc. Please continue to be patient with us if you
are having list issues as we often get busy in our day jobs. If your
message to the list doesn't post and it says it is because you are not a
member, please see if you need to update your email address on the
membership page. Remember you can only post to the list from an email
address that matches exactly with one on the subscriber list. You can
always send us an email at the histonet-owners address and we will try
to help you out. Best wishes for a happy and safe holiday season.
 Linda M
 Histonet administrator
 
 Sent from my iPhone
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[Histonet] PgR Negative Cases for 2014

[Michael Ann Jones]

Did anyone else see an increase of PgR Negative cases early in 2014? Seems like 
there was an increase in the % of cases that were ER + but PgR negative.
We saw an increase in cases particularly from Jan to Jun30th.
Any thoughts?
Thanks in advance,

Michael Ann
Michael Ann Jones, HT (ASCP)
Histology Manager
Metropath
7444 W. Alaska Dr. #250
Lakewood, CO 80226
303.634.2511
mjo...@metropath.com

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Re: [Histonet] RE: Histonet Digest, Vol 132, Issue 10

[Michael Ann Jones]

We use the platinum series from Mercedes Medical - they are so
hydrophilic, that if you place your section wrong on the slide, you cannot
float it off and start over. We also take our sections at a backwards
angle from the surface of the water: rather than lift from underneath - we
put the slide in the water bath next to the section face down at a severe
angle, attach the section to the top of the slide (or wherever) and then
pull slide out at angle. This encourages the water to stay in the bath and
not under sections. Does that make sense?

Michael Ann Jones, HT (ASCP)
Histology Manager
Metropath
7444 W. Alaska Dr. #250
Lakewood, CO 80226
303.634.2511
mjo...@metropath.com






On 11/11/14, 10:48 AM, Richard Bowker richa...@omsc.net wrote:

Judith,

When you pick up your sections are you getting a lot of water trapped
between the tissue and the slides. I have had that problem when getting a
new lot number of slides before and had to change them out. The tissue
wasn't actuall falling off rather then being pushed off after the water
begins to heat up and expand forcing the tissue off. In this instance
shaking the slide after picking up the tissue had no benifit either as
this also caused the water to force the tissue off the slides.

Rick Bowker
Histology supervisor
Olympia Multi-Specialty Clinic
richa...@omsc.net

Message: 5
Date: Sat, 8 Nov 2014 13:31:27 +0300
From: Jamal j.rowa...@alborglaboratories.com
Subject: RE: [Histonet] Tissue falling off positive slides
To: 'Pardue, Judith' judith_par...@memorial.org,
histonet@lists.utsouthwestern.edu
Message-ID:
00c001cffb3f$297ea1f0$7c7be5d0$@rowa...@alborglaboratories.com
Content-Type: text/plain;   charset=windows-1256

Do you use Autostainer?
What brand ?


Best Regards,


Jamal M. Al Rowaihi Anatomic Pathology Supervisor   | Al Borg
Medical Laboratories |? Mobile +966 503629832|
j.rowa...@alborglaboratories.com
Palestine St, Al Rajhi Building, P.O. Box 52817, Jeddah 21573, KSA|
Phone: +966 12 670 0099   | Fax: +966 12 676 4984 |
www.alborglaboratories.com


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Pardue,
Judith
Sent: Thursday, November 06, 2014 6:38 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Tissue falling off positive slides

WE are having trouble with tissue coming off our he slides. Our heater is
set on 65 degrees for 10 minutes. Any suggestions. We use Fisher blue
positive slides.

Judith Pardue
CHI Memorial
Chatt. Tn. 37343
judith_par...@memorial.org

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Re: [Histonet] RE: Squamous cells staining on HE and IHC

[Michael Ann Jones]

We had this issue - particularly for our Keratin stain (as it highlights
the squamous cells) We had much positive staining debris scattered on our
slides.
We cleaned our water baths diligently, made sure our techs were skimming
between slides, and air current and stainers can bring debris as well. We
kind of narrowed it down to one tech maybe? She got frustrated and doesn¹t
cut keratin controls anymore.
Sadly, I¹m not sure how/why this problem subsided. Following this
discussion closely.

Michael Ann Jones, HT (ASCP)
Histology Manager
Metropath
7444 W. Alaska Dr. #250
Lakewood, CO 80226
303.634.2511
mjo...@metropath.com




On 10/29/14, 4:44 AM, Pathology-Histology Sr. Supervisor
hi...@skm.org.pk wrote:

When the sections are cut and taken on the slide from water bath, slide
must only holds on the frosted end of the slides don't touch other part
of slide.
Regards
Muhammad Tahseen
MLT (JIMTEF) Japan
Histology Supervisor
SKMCHRC
Lahore
Pakistan

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Amber
McKenzie
Sent: Wednesday, October 29, 2014 12:55 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Squamous cells staining on HE and IHC


Does anyone else have problems with what looks like squamous cells
staining on your HE's and IHC's?  I'm trying to figure out how to
eliminate that problem in our lab...wear gloves while cutting?  Change
out water bath several times during shifts? Any suggestions?  Thanks!

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Re: [Histonet] Storing specimens

[Michael Ann Jones]

We run a query of reports (finalized) for that period of two weeks, and
any outstanding/pending report, we pull the bottle.
We use the storage bins for each day of the week - during gross we toss
the bottle into the storage bin for that day. We have two sets: Mon - Fri.
We rotate those in two cabinets and have a ³Current² sign we use and move
from one cabinet to another depending on which we dump. Once we dump the
bins (and disinfect them) we keep the Medical Waste bin for another week
(we keep our tissues for a total of three weeks) We don¹t have storage
room for 3 weeks worth rotation.
Did I make any sense?
:)
Michael Ann Jones, HT (ASCP)
Histology Manager
Metropath
7444 W. Alaska Dr. #250
Lakewood, CO 80226
303.634.2511
mjo...@metropath.com




On 10/28/14, 10:04 AM, Abbott, Tanya tanyaabb...@catholichealth.net
wrote:

How does everyone handle storage of specimens for 2 weeks post sign out?
We are a small lab (as is everyone on Histonet, I'm sure!!) and trying to
figure out the best way to organize to make the flow go smoothly.
Meaning, we are hoping not to have to check each specimen to determine it
was signed out before we discard. Any suggestions?
Thanks! Tanya

Tanya G. Abbott RT (CSMLS)
Manager Technologist, Histology/Cytology
St. Joseph Medical Center
Reading, PA 19603-0316
ph  610-378-2635
fax 610-898-5871
email: tanyaabb...@catholichealth.net

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Re: [Histonet] RE: Another TGIF Friday...

[Michael Ann Jones]

Oh boy! Now that¹s funny!!

Michael Ann Jones, HT (ASCP)
Histology Manager
Metropath
7444 W. Alaska Dr. #250
Lakewood, CO 80226
303.634.2511
mjo...@metropath.com




On 10/10/14, 10:35 AM, Weems, Joyce K. joyce.we...@emoryhealthcare.org
wrote:

LAB HIERARCHY

Chief Pathologist
  Leaps tall building in a single bound
 Is more powerful than a locomotive
Is faster than a speeding bullet
   Walks on water,
   Gives policy to God

Associate Pathologist
  Leaps short buildings in single bound
  Is more powerful than a switch engine
Is just as fast as a speeding bullet
  Walks on water if sea is calm
  Talks with God

The Department Head
  Leaps short buildings with a running start and favorable winds
  Is almost as powerful as a switch engine
 Is faster than a speeding bullet
  Walks on water in an indoor pool
  Talks with God if special request is approved

Director of Laboratories
  Rarely clears a Quonset hut
  Loses tug of war with locomotive
Can fire a speeding bullet
 Swims well
 Is occasionally addressed by God

Associate Director of Laboratories
  Makes high marks on the walls when trying to leap tall buildings
  Is run over by locomotives
 Can sometimes handle a gun without inflicting self-injury
Dog paddles
 Talks to animals

Supervisor
 Runs into building
  Recognizes locomotives two out of three times
  Is not issued ammunition
   Can stay afloat with a life jacket
 Talks to walls

Chief Technologist
   Falls over doorstep when trying to enter building
 Says look at the choo-choo
Wets themselves with a water pistol
  Plays in mud puddles
Mumbles to Themselves

Histotechnologist
 Lifts tall buildings and walks under them
   Kicks locomotives off the tracks
 Catches speeding bullets in their teeth and eats them
  Freezes water with a single glance
They are God

Anonymous

Joyce Weems
Pathology Manager
678-843-7376 Phone
678-843-7831 Fax
joyce.we...@emoryhealthcare.org



www.saintjosephsatlanta.org
5665 Peachtree Dunwoody Road
Atlanta, GA 30342

This e-mail, including any attachments is the property of Saint Joseph's
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you are not the intended recipient, please delete this message, and reply
to the sender regarding the error in a separate email.


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
sarah.dys...@stdavids.com
Sent: Friday, October 10, 2014 11:50 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Another TGIF Friday...

Does anyone have that thing handy that says what different positions in
the histology lab do?  It's like Superman, leaping buildings and such...

Sarah E. Dysart, BA, HT (ASCP), QIHC (ASCP) Pathology Supervisor St.
David's North Austin Medical Center
12221 North Mopac Expressway
Austin, Texas  78758
(512)901-1220

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Re: [Histonet] RE: Three shades of Eosin

[Michael Ann Jones]

Terri - agreed. We do the same.
Michael Ann Jones, HT (ASCP)
Histology Manager
Metropath
7444 W. Alaska Dr. #250
Lakewood, CO 80226
303.634.2511
mjo...@metropath.com




On 9/11/14, 12:42 PM, Terri  Braud tbr...@holyredeemer.com wrote:

Eosin is differentiated by water content immediately following the Eosin
stain.  This can be accomplished by a water rinse, or rinse in a diluted
alcohol.  I prefer using 70% Alcohol to 95% Alcohol, then 3 washes of
Absolute, all timed to give the desired differentiation for the 3
desired shades.

Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Holy Redeemer Hospital Laboratory
1648 Huntingdon Pike
Meadowbrook, PA 19046
Ph: 215-938-3676
Fax: 215-938-3874

Sent: Thursday, September 11, 2014 12:09 PM
To: histonet@lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 130, Issue 11

   8. Eosin shades (Manahil)
Date: Thu, 11 Sep 2014 20:15:21 +0400
From: Manahil mbir...@yahoo.com
Subject: [Histonet] Eosin shades
Hi every one
Any one know how to get 3 shades of eosin stain. I am using alcoholic
eosin.
Thanks
Manahil Elbireir
HTL/ ASCP
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[Histonet] RE: biopsy bags for processing - alternatives

[Michael Ann Jones]

Telfa pads? We've used those with success.

Michael Ann Jones, HT(ASCP)
Metropath, Denver, CO

From: histonet-boun...@lists.utsouthwestern.edu 
histonet-boun...@lists.utsouthwestern.edu on behalf of Pam Marcum 
mucra...@comcast.net
Sent: Wednesday, August 13, 2014 9:37 AM
To: Timothy Morken
Cc: Histonet
Subject: Re: [Histonet] biopsy bags for processing - alternatives

Our kidney and liver biopsies are placed in BX bags (tea bags).  The 
pathologist feel the nylon bags leave a pattern on the tissue and sponges are 
even worse.  The Gross Room staff and residents also dislike the nylon bags as 
they feel they are harder to handle and stiff.  Then we in Histology feel 
exactly the same as Tim's description.  We have tried various things and keep 
going bag to tea bag style biopsy bags.  If anyone has come up with a better 
idea or product please let us all know.
Thank You,
Pam Marcum
UAMS

- Original Message -

From: Timothy Morken timothy.mor...@ucsfmedctr.org
To: Histonet histonet@lists.utsouthwestern.edu
Sent: Wednesday, August 13, 2014 10:24:00 AM
Subject: [Histonet] biopsy bags for processing - alternatives

All knowing Histonet,

Our grossing staff uses nylon biopsy bags to enclose some biopsy specimens. 
The embedding staff find them troublesome because when they pull the bags open 
they tend to pop open and throw the tissue off in all directions. They have 
to be very careful opening these. Is there another bag made of some other 
material that is less prone to this problem?

For various reasons some of these samples can't be put on sponges. They do wrap 
some in flat biopsy paper, but not others. It seems to be a grossing personal 
preference more than anything else.

Thanks for any and all info!

Tim Morken
Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies
UC San Francisco Medical Center
Box 1656
505 Parnassus Ave
San Francisco, CA 94143
USA

415.514-6042  (office)
tim.mor...@ucsfmedctr.orgmailto:tim.mor...@ucsfmedctr.org


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Re: [Histonet] On the lighter side...

[Michael Ann Jones]

Ha ha!! Good one - 
Michael Ann



On 8/12/14, 12:58 AM, susan.wal...@hcahealthcare.com
susan.wal...@hcahealthcare.com wrote:

Also heard Histotechs are good embed!!! LOL

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ingles
Claire
Sent: Monday, August 11, 2014 12:00 PM
To: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] On the lighter side...


Old histologists never die, they are just well fixed...
Claire

From: histonet-boun...@lists.utsouthwestern.edu
[histonet-boun...@lists.utsouthwestern.edu] on behalf of Michael Ann
Jones [mjo...@metropath.com]
Sent: Monday, August 11, 2014 9:07 AM
To: Edwards, Richard; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] On the lighter side...

25 years, (what¹s that in micron¹s?²) Bernice, you are too funny!!
(lots of tenure here . . .lotsa brain cells) Michael Ann Jones, HT (ASCP)
Histology Manager Metropath
7444 W. Alaska Dr. #250
Lakewood, CO 80226
303.634.2511
mjo...@metropath.com




On 8/11/14, 5:17 AM, Edwards, Richard r...@leicester.ac.uk wrote:

Sniffed my  first formalin and  saw  first post-mortem November 1965.

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Re: [Histonet] On the lighter side...

[Michael Ann Jones]

25 years, (what¹s that in micron¹s?²) Bernice, you are too funny!!
(lots of tenure here . . .lotsa brain cells)
Michael Ann Jones, HT (ASCP)
Histology Manager
Metropath
7444 W. Alaska Dr. #250
Lakewood, CO 80226
303.634.2511
mjo...@metropath.com




On 8/11/14, 5:17 AM, Edwards, Richard r...@leicester.ac.uk wrote:

Sniffed my  first formalin and  saw  first post-mortem November 1965.


  
  Richard  Edwards

  
   Leicester   U.K.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Morken,
Timothy
Sent: 08 August 2014 19:26
To: 'Douglas Porter'; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] On the lighter side...

Wow, I feel like a newbie! 28 years, registered. HT 13663, 1988, HTL
1369, 1992. Electron Microscopy Technologist, #604, 1982.

Like most, I never heard of histology until I walked into a hospital
lab on my first day as  an EM tech. I had seen slides made in college,
but no one ever mentioned it could be an actual profession. I was more
taken with the electron microscope, and there was (is) a 2-year program
at the community college in the town I grew up in (Delta College,
Stockton, CA). So AFTER getting a BA in Zoology, I went there to get a
marketable skill. At that time EM was still used for tumor dx, so when I
started it was about half tumor, half kidney. I was lucky enough to get
involved in histology and set up the IHC lab at the small community
hospital I worked at (as an EM tech) and so ended up phasing myself
almost out of an EM job. The IHC took over all the tumor dx from EM.
Later I left EM altogether and did IHC exclusively for 15 years. But,
like most, I learned Histotechnology on the job but was lucky enough to
work for a pathologist who believed in developing his techs - to the
point of paying for meetings out of his own pocket. Only now do I know
how fortunate I was to work for someone like that. Because of him we had
developed a culture in the small histo lab (4 men!!) of learning. We
studied together one night a week for the HT exam and all passed (and the
practical!). Again, that was a fortunate experience, not very often seen
in labs. 

Tim Morken
Supervisor, Histology, Electron Microscopy and Neuromuscular Special
Studies UC San Francisco Medical Center San Francisco, CA


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Douglas
Porter
Sent: Thursday, August 07, 2014 11:39 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] On the lighter side...

How long have you been a registered histotech?  36 years here.  You???

 

Douglas A. Porter, HT (ASCP)
Grossing Technician
IT Coordinator

Cancer Registrar 


CAP-Lab, PLC 
2508 South Cedar Street
Lansing, MI 48910-3138

517-372-5520 (phone)
517-372-5540 (fax)

 mailto:doug.por...@caplab.org doug.por...@caplab.org

 http://www.caplab.org/ www.caplab.org

 

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Re: [Histonet] On the lighter side...

[Michael Ann Jones]

Oh,
I ment to say “Lots of tenure here and lots of brain cells here” meaning
this website and all histotechs here, not myself.
Obviously I don’t have many brain cells left If I made it look like I was
talking about myself. . .sheesh. .

Michael Ann Jones, HT (ASCP)
Histology Manager
Metropath
7444 W. Alaska Dr. #250
Lakewood, CO 80226
303.634.2511
mjo...@metropath.com




On 8/11/14, 10:18 AM, Bernice Frederick b-freder...@northwestern.edu
wrote:

It was Friday,I was getting goofy doing paperwork,...

Bernice Frederick HTL (ASCP)
Senior Research Tech
Pathology Core Facility
ECOGPCO-RL
Robert. H. Lurie Cancer Center
Northwestern University
710 N Fairbanks Court
Olson 8-421
Chicago,IL 60611
312-503-3723
b-freder...@northwestern.edu


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Michael
Ann Jones
Sent: Monday, August 11, 2014 9:07 AM
To: Edwards, Richard; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] On the lighter side...

25 years, (what¹s that in micron¹s?²) Bernice, you are too funny!!
(lots of tenure here . . .lotsa brain cells) Michael Ann Jones, HT (ASCP)
Histology Manager Metropath
7444 W. Alaska Dr. #250
Lakewood, CO 80226
303.634.2511
mjo...@metropath.com




On 8/11/14, 5:17 AM, Edwards, Richard r...@leicester.ac.uk wrote:

Sniffed my  first formalin and  saw  first post-mortem November 1965.


 
  Richard  Edwards

 
   Leicester   U.K.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Morken,
Timothy
Sent: 08 August 2014 19:26
To: 'Douglas Porter'; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] On the lighter side...

Wow, I feel like a newbie! 28 years, registered. HT 13663, 1988, HTL
1369, 1992. Electron Microscopy Technologist, #604, 1982.

Like most, I never heard of histology until I walked into a hospital
lab on my first day as  an EM tech. I had seen slides made in college,
but no one ever mentioned it could be an actual profession. I was more
taken with the electron microscope, and there was (is) a 2-year program
at the community college in the town I grew up in (Delta College,
Stockton, CA). So AFTER getting a BA in Zoology, I went there to get a
marketable skill. At that time EM was still used for tumor dx, so when
I started it was about half tumor, half kidney. I was lucky enough to
get involved in histology and set up the IHC lab at the small community
hospital I worked at (as an EM tech) and so ended up phasing myself
almost out of an EM job. The IHC took over all the tumor dx from EM.
Later I left EM altogether and did IHC exclusively for 15 years. But,
like most, I learned Histotechnology on the job but was lucky enough to
work for a pathologist who believed in developing his techs - to the
point of paying for meetings out of his own pocket. Only now do I know
how fortunate I was to work for someone like that. Because of him we
had developed a culture in the small histo lab (4 men!!) of learning.
We studied together one night a week for the HT exam and all passed
(and the practical!). Again, that was a fortunate experience, not very
often seen in labs.

Tim Morken
Supervisor, Histology, Electron Microscopy and Neuromuscular Special
Studies UC San Francisco Medical Center San Francisco, CA


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Douglas
Porter
Sent: Thursday, August 07, 2014 11:39 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] On the lighter side...

How long have you been a registered histotech?  36 years here.  You???

 

Douglas A. Porter, HT (ASCP)
Grossing Technician
IT Coordinator

Cancer Registrar


CAP-Lab, PLC
2508 South Cedar Street
Lansing, MI 48910-3138

517-372-5520 (phone)
517-372-5540 (fax)

 mailto:doug.por...@caplab.org doug.por...@caplab.org

 http://www.caplab.org/ www.caplab.org

 

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Re: [Histonet] RE: Glassware Cleaning, CAP Checklist Item GEN.41770

[Michael Ann Jones]

Ditto with Brian Cooper.
Michael Ann Jones, HT (ASCP)
Histology Manager
Metropath
7444 W. Alaska Dr. #250
Lakewood, CO 80226
303.634.2511
mjo...@metropath.com




On 8/5/14, 1:07 PM, Cooper, Brian bcoo...@chla.usc.edu wrote:

I've never been asked by a CAP auditor either, but our internal
inspectors used to inquire about this in a previous lab all the time.  We
use Bromocresol Purple as an indicator here, and we have a simple log
sheet wherein our crew just tests one piece of cleaned glassware each
day.  They write the type of glassware they test, and place a checkmark
in a yellow or purple column based upon what they see.  If purple,
they rewash, retest and document accordingly.

Brian D. Cooper, HT (ASCP)CM | Histology Supervisor
Department of Pathology and Laboratory Medicine
Children's Hospital Los Angeles
4650 Sunset Blvd MS#43- Los Angeles, CA 90027
Ph: 323.361.3357 Pager: 213-209-0184
bcoo...@chla.usc.edu

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Laurie
Colbert
Sent: Tuesday, August 05, 2014 11:06 AM
To: Histonet Post (histonet@lists.utsouthwestern.edu)
Subject: [Histonet] Glassware Cleaning, CAP Checklist Item GEN.41770

Do others in Histology follow these CAP Guidelines for cleaning
glassware?   I'm not sure if this question actually applies to Histology.
 I have never had an inspector ask me about it, but I'm wondering if
anyone has been questioned.

Laurie Colbert, HT (ASCP)
Histology Supervisor
PATH MD
8158 Beverly Blvd.
Los Angeles, CA  90048
(323) 648-3214 direct
(424) 245-7284 main lab

The information contained in this transmission may contain privileged and
confidential information, including patient information protected by
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person(s) named above. If you are not the intended recipient, you are
hereby notified that any review, dissemination, distribution, or
duplication of this communication is strictly prohibited. If you are not
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[Histonet] Cassette Printer

[Michael Ann Jones]

Has anyone out there used the Primera automated cassette printer? Pros? Cons?
We are close to the General Data model, however, this Primera looks kinda cool.
Thank you for input,
Michael Ann Jones, HT (ASCP)
Histology Manager
Metropath
7444 W. Alaska Dr. #250
Lakewood, CO 80226
303.634.2511
mjo...@metropath.com

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Re: [Histonet] RE: Histonet Digest, Vol 128, Issue 30

[Michael Ann Jones]

Rebecca, 
We use slide tray boxes with large index cards. We have first control
slide - to prove block works. Then pre-cut control slides then one stained
last control slide - to make sure entity is still there. And the block
that is in use is stored in box with the slides. Index card notes control
block, who cut controls, how many, date etc. We keep our slide boxes
alphabetized and stored on shelving for easy use. Slide box is pulled
anytime the controls get to 5 slides or less -
Anytime a block is tested we keep a record (in our procedure manual) as to
whether it is appropriate for that stain or not - it is numbered.
We take block log sheet into pathologist to review controls - log sheet is
a table with date, tissue type, block #, stain employed, acceptable for
use, pathologist or technician initials.

Does that help?

Michael Ann Jones, HT (ASCP)
Histology Manager
Metropath
7444 W. Alaska Dr. #250
Lakewood, CO 80226
303.634.2511
mjo...@metropath.com






On 7/31/14, 2:13 PM, Hayden, Rebecca rhay...@stormontvail.org wrote:

I have recently started to take over the control blocks for our lab
department.  I want to use/make a form that I can show how many blocks I
have approved for each stain and which blocks are in use.  I have looked
a little online to see if there is a form out there that already exists,
but can not seem to find one.  I know that I can make it if needed, but
seeing if anyone knows of one that is already made.  Thanks.


Rebecca Hayden, HTL (ASCP)
Stormont-Vail Healthcare


From: histonet-boun...@lists.utsouthwestern.edu
[histonet-boun...@lists.utsouthwestern.edu] on behalf of
histonet-requ...@lists.utsouthwestern.edu
[histonet-requ...@lists.utsouthwestern.edu]
Sent: Wednesday, July 30, 2014 12:03 PM
To: histonet@lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 128, Issue 30

Send Histonet mailing list submissions to
histonet@lists.utsouthwestern.edu

To subscribe or unsubscribe via the World Wide Web, visit
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or, via email, send a message with subject or body 'help' to
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When replying, please edit your Subject line so it is more specific
than Re: Contents of Histonet digest...


Today's Topics:

   1. Processor Alarms (Cristi Stephenson)
   2. Thank you! (Cristi)


--

Message: 1
Date: Tue, 29 Jul 2014 11:33:35 -0700
From: Cristi Stephenson cls71...@gmail.com
Subject: [Histonet] Processor Alarms
To: histonet@lists.utsouthwestern.edu
Message-ID:

CAMjZ=fzybw-bszym2gazsits3dojuvtz8cuc_1khk80q_zv...@mail.gmail.com
Content-Type: text/plain; charset=UTF-8

Hello Histoland,

We have an ASP300S processor that is not currently monitored by Leica.  I
was wondering if anyone had an alarm system suggestion such that, in the
event it does malfunction, it will notify a designated person.  We are a
private practice so there is no one in the building after normal working
hours.

Thanks in advance for any recommendations!

Cristi


--

Message: 2
Date: Tue, 29 Jul 2014 12:56:52 -0700
From: Cristi cls71...@gmail.com
Subject: [Histonet] Thank you!
To: histonet@lists.utsouthwestern.edu
Message-ID: d28ed65b-66f5-443f-a6b6-11f3f06ed...@gmail.com
Content-Type: text/plain; charset=utf-8

Thank you all for the responses on alarm systems for the processors.  I
knew they were out there but couldn't come up with the right combination
for Google to understand what I was looking for 

Sent from my iPhone


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End of Histonet Digest, Vol 128, Issue 30
*

NEED A DOCTOR?  Stormont-Vail's Health Connections can help you find a
doctor accepting new patients.  Call (785) 354-5225.

**


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Re: [Histonet] Peggy Wenk's passing

[Michael Ann Jones]

So sorry to hear of the loss of a wonderful woman.
Michael Ann



On 7/28/14, 9:10 AM, Damien dml...@laudierhistology.com wrote:

Very sorry to read this. Peggy was truly a wonderful person and will be
missed.

-Damien L.


On Mon, Jul 28, 2014 at 10:57 AM, Colleen Forster cfors...@umn.edu
wrote:

 So very sorry to hear of Peggy's passing. She was such as inspiration
to us
 all. She will be greatly missed. May God grant her family the peace and
 grace to pass into the following days with strength.

 She will now be soaring with the eagles watching over everyone.

 Rest in peace Peggy,

 Colleen Forster


 On Mon, Jul 28, 2014 at 9:47 AM, Tina Van Meter
tina.vanme...@gmail.com
 wrote:

  I totally agree with Pam. I can only hope to leave a fraction of an
  impact when I depart this earth on my colleagues, friends and family
  as Peggy has left with us. She earned her wings a long time ago and is
  now at peace.
 
  Tina Van Meter
 
  On Mon, Jul 28, 2014 at 10:34 AM, Marcum, Pamela A pamar...@uams.edu
  wrote:
   I am sorry to hear of our loss in the Histology world and more
  importantly of a truly good person in our lives.
  
   Pam Marcum
  
   -Original Message-
   From: histonet-boun...@lists.utsouthwestern.edu [mailto:
  histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lee  Peggy
Wenk
   Sent: Monday, July 28, 2014 8:48 AM
   To: Histonet@lists.utsouthwestern.edu
   Subject: [Histonet] Peggy Wenk's passing
  
   It is my sad duty to tell you that Peggy Wenk passed away peacefully
  Saturday morning.  Her husband and companion of 37 years and her
sister
  (from Bend,OR) were there.
  
   As you know Peggy had a large influence in the world of Histology.
  
   She also put herself into her church serving over the years as a
 nursery
  school director, Lay Eucharistic Minister and on the vestry.
   She sang enthusiastically in the choir and played in the newly
formed
  bell choir.
  
   Because of our love of books, we both volunteered at the local
library.
   We helped collect books, sort them and then helped to sell them;
 raising
  funds for the library's use.
  
   There will be two memorial services: one here in Michigan for all
her
  local family and coworkers, the second in southern California (a
burial
 at
  sea).  We are asking that no flowers be sent; instead Peggy has
specified
  (she and her sister planned the funeral several months ago) three
 different
  charitable organizations in lieu of flowers.
  
   St. Mary's-in-the-Hills Episcopal Church (Peggy's church)
  
   Shades of Pink Foundation (financial aid to local breast cancer
 patients)
  
   Peggy A. Wenk Endowed Scholarship for Histotechnology at Oakland
  University
  
   Please respond to this email if you'd like more information on the
  services or on giving (or to l...@lpwenk.net).
  
   Thanks
   Lee Wenk (Mr. Peggy Wenk;)
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-- 
Damien Laudier
Laudier Histology
www.LaudierHistology.com
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Re: [Histonet] Coverslip

[Michael Ann Jones]

We have found this to be a culmination of a couple of things:
Bad lot of tape from vendor
Drying slides before cover slipping
Too much xylene drip during coverslip (dilutes the glue stuff on the tape
allowing for weak adhesive)

We use Sakura cover slipping tape for more consistent quality and adjust
xylene drip accordingly and make sure slides do not sit out very long at
all as xylene from the stainer will evaporate (drying slides) rather
quickly.
Hope this helps.

Michael Ann Jones, HT (ASCP)
Histology Manager
Metropath
7444 W. Alaska Dr. #250
Lakewood, CO 80226
303.634.2511
mjo...@metropath.com




On 7/25/14, 7:47 AM, Tommy Mai t...@phenopath.com wrote:

Hey everyone,

I was wondering if anyone else has seen this occur in their labs and how
to resolve this matter.  It has to do with the coverslip using the resin
tape.  However more recently, I have been noticing that there is a drying
artifact that happens right as the xylene drys.  It looks almost like
when water freezes and gives that frosted look.

Thanks,
Tommy

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Re: [Histonet] Re: IHC Validation

[Michael Ann Jones]

I document all of the worksheet process¹ and then I create a ³Validation
Report² where my technical director both reviews all slides and signs this
report.
Michael Ann Jones, HT (ASCP)
Histology Manager
Metropath
7444 W. Alaska Dr. #250
Lakewood, CO 80226
303.634.2511
mjo...@metropath.com




On 7/15/14, 7:51 AM, Leslie Graves lsgrave...@gmail.com wrote:

Thank you to everyone who sent a template.  This is very helpful.  I do
need clarfication on the 10 cases that are required for validation.  Do
you
include the 10 cases on this same form, or do you have something separate
for that?  These look ideal for optimization.  From what I have read
online, 10 cases need to be run after optimization has been completed.
When validating these 10 cases, accuracy, sensitivity, specificity and
reproducibility need to be monitored.  Are all of you using separate form
to capture this data and approval from the Pathologists?  Maybe I am going
overboard, but I want to make sure that I have everything covered.  We are
bringing a new platform into our lab in 2 weeks, and I want to have
everything ready to go.

Leslie Graves
Technical Specialist
Cape Fear Valley Medical Center
Fayetteville, NC  28341
910-615-6142
lg...@capefearvalley.com

On Mon, Jul 14, 2014 at 1:55 PM, Leslie Graves lsgrave...@gmail.com
wrote:

 Does anyone have a sample IHC Validation worksheet that they used in
their
 lab for review.  I know that the requirements have changed since our
last
 inspection.  I want to make sure that I am including all of the
criteria,
 and would like to see how other labs are documenting this process.

 Leslie Graves
 Technical Specialist
 Cape Fear Valley Medical Center
 1638 Owen Drive
 Fayetteville, NC 28341
 910-615-6142
 lg...@capefearvalley.com

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Re: [Histonet] Microtome calibration

[Michael Ann Jones]

We do ours by hand? You mean line them up or PM?
Michael Ann Jones, HT (ASCP)
Histology Manager
Metropath
7444 W. Alaska Dr. #250
Lakewood, CO 80226
303.634.2511
mjo...@metropath.com




On 7/15/14, 1:55 PM, Ronda Mire rm...@cvpath.org wrote:

Hi Denise,

Check with the company that does your PM service.  This may be something
that is already done during preventative maintenance.

On Jul 15, 2014, at 3:02 PM, Long, Denise denise.l...@uconn.edu wrote:

 Hi Everyone,
 During a recent inspection we were asked by the inspection team to
start calibrating our microtomes.  Anyone know of a service vendor that
will do this? Referrals, including self-referrals would be greatly
appreciated.
 Thank,
 
 Denise M. Long, MS, HTL (ASCP), QIHC
 University of Connecticut
 Dept. of Pathobiology and Veterinary Sciences
 Connecticut Veterinary Medical Diagnostic Laboratory
 61 N. Eagleville Road, Unit 3089
 Storrs, Connecticut 06269-3089
 (860) 486-0851
 
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Re: [Histonet] Sekura Tissue Tek Block Storage

[Michael Ann Jones]

We re-use block file drawers as well. We write on the front with red
sharpie marker and take off writing with alcohol.
Works pretty well.
Michael Ann Jones, HT (ASCP)
Histology Manager
Metropath
7444 W. Alaska Dr. #250
Lakewood, CO 80226
303.634.2511
mjo...@metropath.com




On 7/8/14, 11:26 AM, Sheila Haas sm...@msn.com wrote:

As we reuse block file drawers, we run a piece of bold colored tape (as
wide as the front of the drawer) across
the front. We use a different color for each year which helps quickly
distinguish one year from the next when pulling or filing blocks. Works
very well here and is inexpensive.
 
Good luck.
Sheila Haas
MicroPath Laboratories, Inc.
 
 
 
 From: ning...@kaleidahealth.org
 To: histonet@lists.utsouthwestern.edu
 Date: Tue, 8 Jul 2014 17:23:19 +
 Subject: [Histonet] Sekura Tissue Tek Block Storage
 
 Hello Histonet,
 
 First time poster. My institution has a 25 year retention on paraffin
blocks. The 1989 batch was just destroyed and the storage containers
were returned to me. They appear to be the same size as the current
sekura tissue block storage units we use. Does anyone know of a
commercially available label overlay for the drawers? I could cut down
adhesive labels, but wanted to see what was out there on the market.
 
 Thanks, Nick
 
 
 Nick Ingrao, MBA, MT(ASCP)
 Manager, Anatomic Pathology
 Kaleida Health: Buffalo General Medical Center
 100 High St
 Buffalo, NY 14203
 
 Office:(716) 859-2028
 Pager: (716) 642-0232
 Fax: (716) 859-2393
 
 
 
 The Keeping You Informed section of Kaleida Health`s website features a
wealth of information, stories and pictures about our valued workforce
and the tremendous momentum our organization is experiencing. Check us
out at: www.kaleidahealth.org/kyi
 
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Re: [Histonet] RE: Re: Friday histology trivia

[Michael Ann Jones]

Ha! I can beat that~ we have a cryostat that is at least 50+ years old,
old AO microtome cryostat. Still works, barely. . .looks just like the old
fashion ice-cream holders. (P.S. Prob not true to life, but I liked
Quincy-did I just date myself?)

Michael Ann Jones, HT (ASCP)
Histology Manager
Metropath
7444 W. Alaska Dr. #250
Lakewood, CO 80226
303.634.2511
mjo...@metropath.com




On 6/27/14, 12:35 PM, Mike Andrews udsd...@gmail.com wrote:

One of my current is the (Salonpas?) ad I which there is a very obvious
AO Series 10 just behind the presenter. Good old brass'n'glass, but
hardly current -- even if I do own a few.

Mike Andrews, W5EGO
WWME Oklahoma area executive team

 On Jun 27, 2014, at 12:25 PM, Morken, Timothy
timothy.mor...@ucsfmedctr.org wrote:
 
 Are all news stories are as faked as the ones showing something in a
lab? One had two doctors(?) in lab coats peering at a microscope slide
they are holding to the light above their head and the reporter is
saying they are examining samples from a cancer patient.
 
 Wow, good eyesight!
 
 Tim Morken
 Supervisor, Electron Microscopy and Neuromuscular Special Studies
 UC San Francisco Medical Center
 San Francisco, CA
 
 -Original Message-
 From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Teri
Johnson
 Sent: Friday, June 27, 2014 10:15 AM
 To: histonet@lists.utsouthwestern.edu
 Subject: [Histonet] Re: Friday histology trivia
 
 Carefully placed scientific equipment, as well as spokespeople dressed
in lab coats, are great marketing tools for anything touted to cure us
or make us healthier.
 
 Next time you watch an actual medical or research-based news story,
check out how they ALWAYS show someone pipetting. Always.
 It's become a game for me to spot it.
 
 Teri Johnson
 Manager, Histology
 Genomics Institute for
 Novartis Research
 Foundation
 San Diego, CA
 858-332-4752
 
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Re: [Histonet] G.I. biopsies and special stains

[Michael Ann Jones]

We order HP by IHC automatically on all stomach and GE juction specimens.
Michael Ann Jones, HT (ASCP)
Histology Manager
Metropath
7444 W. Alaska Dr. #250
Lakewood, CO 80226
303.634.2511
mjo...@metropath.com




On 6/9/14 7:14 AM, kathy.mach...@lpnt.net kathy.mach...@lpnt.net wrote:

I was wondering if other labs are automatically ordering special stains
and/or IHC on stomach biopsies and esophageal biopsies?  Or do you wait
until HE is screened?  Thanks!

Danville Regional Medical Center
Danville, VA
kathy.mach...@lpnt.netmailto:kathy.mach...@lpnt.net
434-799-3868

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Re: [Histonet] G.I. biopsies and special stains

[Michael Ann Jones]

We bill all of the stomach HP, but we do not bill all of the GE specimens.
Only those deemed necessary (if we even do them - some pathologists do not
want them, some do)
Michael Ann Jones, HT (ASCP)
Histology Manager
Metropath
7444 W. Alaska Dr. #250
Lakewood, CO 80226
303.634.2511
mjo...@metropath.com




On 6/9/14 8:30 AM, Rathborne, Toni toni.rathbo...@rwjuh.edu wrote:

We wait for HE.
For the labs that order automatically, do you bill all of them, or just
the ones determined to be necessary?


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Michael
Ann Jones
Sent: Monday, June 09, 2014 9:38 AM
To: kathy.mach...@lpnt.net; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] G.I. biopsies and special stains

We order HP by IHC automatically on all stomach and GE juction specimens.
Michael Ann Jones, HT (ASCP)
Histology Manager
Metropath
7444 W. Alaska Dr. #250
Lakewood, CO 80226
303.634.2511
mjo...@metropath.com




On 6/9/14 7:14 AM, kathy.mach...@lpnt.net kathy.mach...@lpnt.net
wrote:

I was wondering if other labs are automatically ordering special
stains and/or IHC on stomach biopsies and esophageal biopsies?  Or do
you wait until HE is screened?  Thanks!

Danville Regional Medical Center
Danville, VA
kathy.mach...@lpnt.netmailto:kathy.mach...@lpnt.net
434-799-3868

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Re: [Histonet] Validating slides

[Michael Ann Jones]

P.S. We use Platinum series from Mercedes Medical (so far we're okay)
previously we used Fisher Superfrost +, good quality, however these were
very expensive.

Michael Ann Jones, HT (ASCP)
Histology Manager
Metropath
7444 W. Alaska Dr. #250
Lakewood, CO 80226
303.634.2511
mjo...@metropath.com




On 6/4/14 4:48 PM, Sharon Scalise sscal...@beaumont.edu wrote:

I am looking for comments about validating slides in histology/immuno.
We have always validated our charged slides if we went to a new
brand/vendor in order to ensure that the tissue sections will adhere
properly during immuno procedures and certain special stains.  Once we
found them to be acceptable, we continue to use them, lot to lot, and
never validate as long as we do not change brand/vendor.  We recently ran
into a problem with slides that we received by accident (similar
packaging, different slide) and they were used for some immuno cases.
The sections on many of the slides fell off and the stains had to be
repeated.  We realized the problem and pulled the slides out of service.
We contacted the vendor and they said that those slides should have been
pulled from the shelf, that it was a bad batch and they should have never
been sent to anyone, yet alone incorrectly sent to us.
I started to think that we might want to consider validating new lots we
receive to ensure that each lot is acceptable.  As we began this
discussion there were people who felt that in any lot you could have
slides that are acceptable and maybe some boxes that give you problems.
How do you know if all slides in one lot are treated exactly the same?
Can you really use lot number to verify quality when it comes to slides?
I am not exactly sure of the process used to charge the slides so I
don't feel qualified to answer these questions.
What is everyone else doing about slide validation?  Is it really
necessary? (I can say that in 30 years I have never seen it done with
each new lot, but I have been at the same job for the past 18 years).

Thanks for your input!

Sharon Scalise, HTL(ASCP)
Histology Supervisor-Anatomic Pathology
Beaumont Health System
3601 W. 13 Mile Rd.
Royal Oak, MI 48073
248 898-5981
sscal...@beaumont.edumailto:sscal...@beaumont.edu


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Re: [Histonet] Validating slides

[Michael Ann Jones]

My experience with slide quality is that no matter who or where you get
your slides from you can have variability even within the same pack. We
found the slide we felt to be the most consistent high quality and we use
that never checking lot to lot validity. An immuno technical specialist
showed us how you can quickly check the quality of the charge on slides
(you may already know this). He took a slide and dipped it into
hematoxylin and then laid it on the counter. You can actually see the heme
being repelled by the poor quality slide, whereas the heme on the higher
quality slide uniformly coated the entire slide. We took 3 or 4 slides
from the pack (from front, middle, and back) to test them for consistency.
Try it, its amazing that you can see the poor slide repelling the heme and
that's what it does to your immuno reagents. You may use this when
determining which slides work best for your lab. Hope this helps a little.

Michael Ann Jones, HT (ASCP)
Histology Manager
Metropath
7444 W. Alaska Dr. #250
Lakewood, CO 80226
303.634.2511
mjo...@metropath.com



On 6/4/14 4:48 PM, Sharon Scalise sscal...@beaumont.edu wrote:

I am looking for comments about validating slides in histology/immuno.
We have always validated our charged slides if we went to a new
brand/vendor in order to ensure that the tissue sections will adhere
properly during immuno procedures and certain special stains.  Once we
found them to be acceptable, we continue to use them, lot to lot, and
never validate as long as we do not change brand/vendor.  We recently ran
into a problem with slides that we received by accident (similar
packaging, different slide) and they were used for some immuno cases.
The sections on many of the slides fell off and the stains had to be
repeated.  We realized the problem and pulled the slides out of service.
We contacted the vendor and they said that those slides should have been
pulled from the shelf, that it was a bad batch and they should have never
been sent to anyone, yet alone incorrectly sent to us.
I started to think that we might want to consider validating new lots we
receive to ensure that each lot is acceptable.  As we began this
discussion there were people who felt that in any lot you could have
slides that are acceptable and maybe some boxes that give you problems.
How do you know if all slides in one lot are treated exactly the same?
Can you really use lot number to verify quality when it comes to slides?
I am not exactly sure of the process used to charge the slides so I
don't feel qualified to answer these questions.
What is everyone else doing about slide validation?  Is it really
necessary? (I can say that in 30 years I have never seen it done with
each new lot, but I have been at the same job for the past 18 years).

Thanks for your input!

Sharon Scalise, HTL(ASCP)
Histology Supervisor-Anatomic Pathology
Beaumont Health System
3601 W. 13 Mile Rd.
Royal Oak, MI 48073
248 898-5981
sscal...@beaumont.edumailto:sscal...@beaumont.edu


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