[Histonet] Mounting medium
I’m trying to find a really rapid drying mounting medium for same day shipping does anyone have any suggestions? When reading they usually just say “rapist dry” but how long is “rapid” Thanks for the help awesome histopeeps! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] mounting medium
Refrax from Anatech -Original Message- From: Allan Wang via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Monday, November 13, 2017 5:47 PM To: warda hassan Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] mounting medium Hi, This information would be very useful for me and probably others as well, since I've just arbitrarily chosen one. Can you summarize the responses you received for us? Allan On Wed, Nov 8, 2017 at 10:28 AM, warda hassan via Histonet < histonet@lists.utsouthwestern.edu> wrote: > Hello to all > > Can anyone suggest which mouting medium is best that will help > preservation of staining properties without creating fading of stains > and bubbles on long run. > Thanks alot > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] mounting medium
We use Sub-X Mounting Medium from Leica. This is what the pathologist wants us to use. -Original Message- From: Allan Wang via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Monday, November 13, 2017 5:47 PM To: warda hassan Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] mounting medium Hi, This information would be very useful for me and probably others as well, since I've just arbitrarily chosen one. Can you summarize the responses you received for us? Allan On Wed, Nov 8, 2017 at 10:28 AM, warda hassan via Histonet < histonet@lists.utsouthwestern.edu> wrote: > Hello to all > > Can anyone suggest which mouting medium is best that will help preservation > of staining properties without creating fading of stains and bubbles on > long run. > Thanks alot > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwICAg&c=imBPVzF25OnBgGmVOlcsiEgHoG1i6YHLR0Sj_gZ4adc&r=eLj2B87Mkll0NUAmalTD_1fRsqzhM-r-54bMn0ykFbs&m=qAeXtP7MVtiB5tSOrrA22EAPWyiHIuG7ijlXdENpf68&s=-1VDk505lt6WIaFntwlARL_RqLOgFH02KVXBwp7kovE&e= > > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwICAg&c=imBPVzF25OnBgGmVOlcsiEgHoG1i6YHLR0Sj_gZ4adc&r=eLj2B87Mkll0NUAmalTD_1fRsqzhM-r-54bMn0ykFbs&m=qAeXtP7MVtiB5tSOrrA22EAPWyiHIuG7ijlXdENpf68&s=-1VDk505lt6WIaFntwlARL_RqLOgFH02KVXBwp7kovE&e= ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] mounting medium
We use Permaslip acrylic for manual coverslipping. Sakura media for our automated coverslipper. We have also used Richard-Allan CytoSeal with great resuls. We stopped using one called Quick-mount (comes in tube) because they had a batch that was bad and the media turned cloudy and the slips dropped off after about 6 months. That has been a pain Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -Original Message- From: Reilly, Laurie via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Monday, November 13, 2017 3:35 PM To: Allan Wang; warda hassan; Histonet@lists.utsouthwestern.edu Cc: Hautaniemi, Walter; Reilly, Sue; Reeks, Karen Subject: Re: [Histonet] mounting medium We also would be interested in other's thoughts on mounting media. We are having coverslips coming unstuck from some of our teaching slides after 3 or 4 years. It is very frustrating when cover slipping manually and wondering how long it will last. Thanks and regards, Laurie. Mr. Laurie REILLY Histopathology Veterinary and Biomedical Sciences James Cook University Townsville Qld. 4811 Australia. Phone 07 4781 4468 Mobile 0448 957747 -Original Message- From: Allan Wang via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Tuesday, 14 November 2017 8:47 AM To: warda hassan Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] mounting medium Hi, This information would be very useful for me and probably others as well, since I've just arbitrarily chosen one. Can you summarize the responses you received for us? Allan On Wed, Nov 8, 2017 at 10:28 AM, warda hassan via Histonet < histonet@lists.utsouthwestern.edu> wrote: > Hello to all > > Can anyone suggest which mouting medium is best that will help > preservation of staining properties without creating fading of stains > and bubbles on long run. > Thanks alot > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] mounting medium
We also would be interested in other's thoughts on mounting media. We are having coverslips coming unstuck from some of our teaching slides after 3 or 4 years. It is very frustrating when cover slipping manually and wondering how long it will last. Thanks and regards, Laurie. Mr. Laurie REILLY Histopathology Veterinary and Biomedical Sciences James Cook University Townsville Qld. 4811 Australia. Phone 07 4781 4468 Mobile 0448 957747 -Original Message- From: Allan Wang via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Tuesday, 14 November 2017 8:47 AM To: warda hassan Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] mounting medium Hi, This information would be very useful for me and probably others as well, since I've just arbitrarily chosen one. Can you summarize the responses you received for us? Allan On Wed, Nov 8, 2017 at 10:28 AM, warda hassan via Histonet < histonet@lists.utsouthwestern.edu> wrote: > Hello to all > > Can anyone suggest which mouting medium is best that will help > preservation of staining properties without creating fading of stains > and bubbles on long run. > Thanks alot > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] mounting medium
Hi, This information would be very useful for me and probably others as well, since I've just arbitrarily chosen one. Can you summarize the responses you received for us? Allan On Wed, Nov 8, 2017 at 10:28 AM, warda hassan via Histonet < histonet@lists.utsouthwestern.edu> wrote: > Hello to all > > Can anyone suggest which mouting medium is best that will help preservation > of staining properties without creating fading of stains and bubbles on > long run. > Thanks alot > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] mounting medium
Hello to all Can anyone suggest which mouting medium is best that will help preservation of staining properties without creating fading of stains and bubbles on long run. Thanks alot ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] mounting medium for DAB & AP chromogens
Hello, I'm currently using an ancient bottle of xylene based mounting medium and want to get something better that will serve multiple purposes. In the past I had used entellan for DAB and routine histo stains (i.e. H&E) but then a student accidentally (recklessly?) used my Mowiol + n-propyl gallate mounting medium (that I use for fluorescence) for a PTAH stain and it is worked well, retaining the sharpness of the stain much longer than the entellan did. In the past I had used the Mowiol for DAB and that seemed to work well. I am now preparing to perform staining with AEC and Fastred that don't like the alcohol dehydration steps needed for a xylene mounting medium. Is there any reason why I can't simply use the Mowiol mounting medium for all my work? It isn't like the DAB is going to dissolve... I am using methyl green as a counter stain for my current work. The mowiol mounting medium is glycerol based ( http://cshprotocols.cshlp.org/content/2006/1/pdb.rec10255.full?text_only=true with n-propyl gallate instead of DABCO). Thanks -- Tyrone Genade Orange City, Iowa tel: (+1) 712 230 4101 http://tgenade.freeshell.org Romans 6:23: The gift of God is eternal life through Christ Jesus our Lord. To find out how to receive this FREE gift visit http://www.alpha.org. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Mounting medium for IF
Hello Laurie Pi gives red color in IF, it stains DNA and RNA PI excites at 535nmand emits at 615nm, producing a *red* fluorescence. Therefore if you are using any chromophore at these wave lengths which emits red color you will have problems. By the way we also have several mounting mediums for IHC and IF, if you would like to try, please let us know. Bader ** -- If any Q's please feel free to contact us Have a nice day/weekend Mit freundlichen Grüßen / With Kind Regards / avec l'aimable ce qui concerne Met vriendelijke groeten 種とについて Bader Bader B Siddiki, PhD Executive director, Research and development ImmunoBioScience corp. Phone: + 425 367 4601 Phone: + 425 514 3761 Fax: + 425 367 4817 cell (mobile) phone: + 425 314 0199 e-mail address: bade...@gmail.com Web site: www.immunobioscience.com Marketing: phone: + 650 343 IBSC (4272) E-mail: anitai...@aol.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Mounting Medium for Immunofluorescence
Actually if I could make a minor correction to your statement: propidium iodide is excited by green light at 488nm but emits in the red portion of the spectrum (620nm)... --On Thursday, May 20, 2010 12:48 PM -0600 Liz Chlipala wrote: I think that the propidium iodide will stain the nuclei or DNA, similar to DAPI, so this mounting media will counterstain the nuclei and its visible in the FITC wavelength (488), while if you conterstain with DAPI it is excited with a different wavelength, therefore it might interfere with the FITC portion of your stain. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert Sent: Thursday, May 20, 2010 12:13 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Mounting Medium for Immunofluorescence We normally coverslip our immunofluorescence slides with a permanent aqueous mounting medium. By mistake, today they were coverslipped with a mounting medium that we use for coverslipping our FISH slides. This mounting medium is described as "Mounting medium for fluorescence, with propidium iodide." Will this make a difference when reading the slides?? Laurie Colbert ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Merced M Leiker Research Technician III Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA lei...@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Mounting Medium for Immunofluorescence
Hi Laurie, Propidium iodide is a DNA intercalator that fluoresces red (ex 488nm). Regards, Merced --On Thursday, May 20, 2010 11:13 AM -0700 Laurie Colbert wrote: We normally coverslip our immunofluorescence slides with a permanent aqueous mounting medium. By mistake, today they were coverslipped with a mounting medium that we use for coverslipping our FISH slides. This mounting medium is described as "Mounting medium for fluorescence, with propidium iodide." Will this make a difference when reading the slides?? Laurie Colbert ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Merced M Leiker Research Technician III Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA lei...@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Mounting Medium for Immunofluorescence
I think that the propidium iodide will stain the nuclei or DNA, similar to DAPI, so this mounting media will counterstain the nuclei and its visible in the FITC wavelength (488), while if you conterstain with DAPI it is excited with a different wavelength, therefore it might interfere with the FITC portion of your stain. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert Sent: Thursday, May 20, 2010 12:13 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Mounting Medium for Immunofluorescence We normally coverslip our immunofluorescence slides with a permanent aqueous mounting medium. By mistake, today they were coverslipped with a mounting medium that we use for coverslipping our FISH slides. This mounting medium is described as "Mounting medium for fluorescence, with propidium iodide." Will this make a difference when reading the slides?? Laurie Colbert ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Mounting Medium for Immunofluorescence
We normally coverslip our immunofluorescence slides with a permanent aqueous mounting medium. By mistake, today they were coverslipped with a mounting medium that we use for coverslipping our FISH slides. This mounting medium is described as "Mounting medium for fluorescence, with propidium iodide." Will this make a difference when reading the slides?? Laurie Colbert ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Mounting Medium Issues
Jim, I must say that I have used Eukitt exclusively for over 13 years and the only problem I have had with Eukitt is when I do not use enough and especially when coverslipping thicker sections. It is xylenes based so maybe you are experiencing excessive evaporation during drying??? If you are diluting the Eukitt with xylenes so that it flows better (not as viscious) and reduces the chance for air bubbles, this might cause excessive shrinkage and/or the problems you are experiencing. Jack On May 14, 2010, at 3:22 PM, "Herrick, James L. (Jim)" > wrote: Hello everyone, Hope you have all had a good week!! Has anyone had any negative experiences using Eukitt as your mounting media? We embed our specimens (human - iliac crest, animal - femurs, tibias, vertebrae, etc.) in GMA and MMA and have noticed over a period of time that a fairly large number of our slides are beginning to show signs of the medium breaking up below the cover slip. It looks as if there are large air pockets left behind. We are not yet sure why this is happening. Would any of you geniuses have a resolution for us or know of a better mounting media to use with plastic embedded specimens - brightfield and fluorescence? Thanks for your expertise. It is very much appreciated. Have a great weekend!! Jim ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
FW: [Histonet] Mounting Medium Issues
-Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Herrick, James L. (Jim) Sent: Friday, May 14, 2010 3:23 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Mounting Medium Issues Hello everyone, Hope you have all had a good week!! Has anyone had any negative experiences using Eukitt as your mounting media? We embed our specimens (human - iliac crest, animal - femurs, tibias, vertebrae, etc.) in GMA and MMA and have noticed over a period of time that a fairly large number of our slides are beginning to show signs of the medium breaking up below the cover slip. It looks as if there are large air pockets left behind. We are not yet sure why this is happening. Would any of you geniuses have a resolution for us or know of a better mounting media to use with plastic embedded specimens - brightfield and fluorescence? Thanks for your expertise. It is very much appreciated. Have a great weekend!! Jim ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Mounting Medium Issues
Hello everyone, Hope you have all had a good week!! Has anyone had any negative experiences using Eukitt as your mounting media? We embed our specimens (human - iliac crest, animal - femurs, tibias, vertebrae, etc.) in GMA and MMA and have noticed over a period of time that a fairly large number of our slides are beginning to show signs of the medium breaking up below the cover slip. It looks as if there are large air pockets left behind. We are not yet sure why this is happening. Would any of you geniuses have a resolution for us or know of a better mounting media to use with plastic embedded specimens - brightfield and fluorescence? Thanks for your expertise. It is very much appreciated. Have a great weekend!! Jim ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
