[Histonet] Mounting medium

2019-06-01 Thread Heather Marlatt via Histonet
I’m trying to find a really rapid drying mounting medium for same day
shipping does anyone have any suggestions?

When reading they usually just say “rapist dry” but how long is “rapid”

Thanks for the help awesome histopeeps!
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Re: [Histonet] mounting medium

2017-11-14 Thread Mary Lou Norman via Histonet
Refrax from Anatech

-Original Message-
From: Allan Wang via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Monday, November 13, 2017 5:47 PM
To: warda hassan
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] mounting medium

Hi,

This information would be very useful for me and probably others as well, since 
I've just arbitrarily chosen one.
Can you summarize the responses you received for us?

Allan

On Wed, Nov 8, 2017 at 10:28 AM, warda hassan via Histonet < 
histonet@lists.utsouthwestern.edu> wrote:

> Hello to all
>
> Can anyone suggest which mouting medium is best that will help 
> preservation of staining properties without creating fading of stains 
> and bubbles on long run.
> Thanks alot
> ___
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>
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Re: [Histonet] mounting medium

2017-11-14 Thread Diane Satterfield via Histonet
We use Sub-X Mounting Medium from Leica.  This is what the pathologist wants us 
to use.

-Original Message-
From: Allan Wang via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Monday, November 13, 2017 5:47 PM
To: warda hassan
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] mounting medium

Hi,

This information would be very useful for me and probably others as well,
since I've just arbitrarily chosen one.
Can you summarize the responses you received for us?

Allan

On Wed, Nov 8, 2017 at 10:28 AM, warda hassan via Histonet <
histonet@lists.utsouthwestern.edu> wrote:

> Hello to all
>
> Can anyone suggest which mouting medium is best that will help preservation
> of staining properties without creating fading of stains and bubbles on
> long run.
> Thanks alot
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwICAg&c=imBPVzF25OnBgGmVOlcsiEgHoG1i6YHLR0Sj_gZ4adc&r=eLj2B87Mkll0NUAmalTD_1fRsqzhM-r-54bMn0ykFbs&m=qAeXtP7MVtiB5tSOrrA22EAPWyiHIuG7ijlXdENpf68&s=-1VDk505lt6WIaFntwlARL_RqLOgFH02KVXBwp7kovE&e=
>  
>
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Re: [Histonet] mounting medium

2017-11-13 Thread Morken, Timothy via Histonet
We use Permaslip acrylic for manual coverslipping. Sakura media for our 
automated coverslipper. We have also used Richard-Allan CytoSeal with great 
resuls. We stopped using one called Quick-mount (comes in  tube) because they 
had a batch that was bad and the media turned cloudy and the slips dropped off 
after about 6 months. That has been a pain

Tim Morken
Pathology Site Manager, Parnassus 
Supervisor, Electron Microscopy/Neuromuscular Special Studies
Department of Pathology
UC San Francisco Medical Center

-Original Message-
From: Reilly, Laurie via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Monday, November 13, 2017 3:35 PM
To: Allan Wang; warda hassan; Histonet@lists.utsouthwestern.edu
Cc: Hautaniemi, Walter; Reilly, Sue; Reeks, Karen
Subject: Re: [Histonet] mounting medium

We also would be interested in other's thoughts on mounting media. We are 
having coverslips coming unstuck from some of our teaching slides after 3 or 4 
years. It is very frustrating when cover slipping manually and wondering how 
long it will last.

Thanks and regards, Laurie.

Mr. Laurie REILLY
Histopathology
Veterinary and Biomedical Sciences
James Cook University
Townsville  Qld.  4811
Australia.

Phone 07 4781 4468
Mobile 0448 957747


-Original Message-
From: Allan Wang via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Tuesday, 14 November 2017 8:47 AM
To: warda hassan 
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] mounting medium

Hi,

This information would be very useful for me and probably others as well, since 
I've just arbitrarily chosen one.
Can you summarize the responses you received for us?

Allan

On Wed, Nov 8, 2017 at 10:28 AM, warda hassan via Histonet < 
histonet@lists.utsouthwestern.edu> wrote:

> Hello to all
>
> Can anyone suggest which mouting medium is best that will help 
> preservation of staining properties without creating fading of stains 
> and bubbles on long run.
> Thanks alot
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
___
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Re: [Histonet] mounting medium

2017-11-13 Thread Reilly, Laurie via Histonet
We also would be interested in other's thoughts on mounting media. We are 
having coverslips coming unstuck from some of our teaching slides after 3 or 4 
years. It is very frustrating when cover slipping manually and wondering how 
long it will last.

Thanks and regards, Laurie.

Mr. Laurie REILLY
Histopathology
Veterinary and Biomedical Sciences
James Cook University
Townsville  Qld.  4811
Australia.

Phone 07 4781 4468
Mobile 0448 957747


-Original Message-
From: Allan Wang via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Tuesday, 14 November 2017 8:47 AM
To: warda hassan 
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] mounting medium

Hi,

This information would be very useful for me and probably others as well, since 
I've just arbitrarily chosen one.
Can you summarize the responses you received for us?

Allan

On Wed, Nov 8, 2017 at 10:28 AM, warda hassan via Histonet < 
histonet@lists.utsouthwestern.edu> wrote:

> Hello to all
>
> Can anyone suggest which mouting medium is best that will help 
> preservation of staining properties without creating fading of stains 
> and bubbles on long run.
> Thanks alot
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
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Re: [Histonet] mounting medium

2017-11-13 Thread Allan Wang via Histonet
Hi,

This information would be very useful for me and probably others as well,
since I've just arbitrarily chosen one.
Can you summarize the responses you received for us?

Allan

On Wed, Nov 8, 2017 at 10:28 AM, warda hassan via Histonet <
histonet@lists.utsouthwestern.edu> wrote:

> Hello to all
>
> Can anyone suggest which mouting medium is best that will help preservation
> of staining properties without creating fading of stains and bubbles on
> long run.
> Thanks alot
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
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[Histonet] mounting medium

2017-11-08 Thread warda hassan via Histonet
Hello to all

Can anyone suggest which mouting medium is best that will help preservation
of staining properties without creating fading of stains and bubbles on
long run.
Thanks alot
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[Histonet] mounting medium for DAB & AP chromogens

2016-06-07 Thread Tyrone Genade via Histonet
Hello,

I'm currently using an ancient bottle of xylene based mounting medium and
want to get something better that will serve multiple purposes.

In the past I had used entellan for DAB and routine histo stains (i.e. H&E)
but then a student accidentally (recklessly?) used my Mowiol + n-propyl
gallate mounting medium (that I use for fluorescence) for a PTAH stain and
it is worked well, retaining the sharpness of the stain much longer than
the entellan did. In the past I had used the Mowiol for DAB and that seemed
to work well. I am now preparing to perform staining with AEC and Fastred
that don't like the alcohol dehydration steps needed for a xylene mounting
medium. Is there any reason why I can't simply use the Mowiol mounting
medium for all my work? It isn't like the DAB is going to dissolve... I am
using methyl green as a counter stain for my current work.

The mowiol mounting medium is glycerol based (
http://cshprotocols.cshlp.org/content/2006/1/pdb.rec10255.full?text_only=true
with n-propyl gallate instead of DABCO).

Thanks
-- 
Tyrone Genade
Orange City, Iowa
tel: (+1) 712 230 4101
http://tgenade.freeshell.org

Romans 6:23: The gift of God is eternal life through Christ Jesus our Lord.
To find out how to receive this FREE gift visit http://www.alpha.org.
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[Histonet] Mounting medium for IF

2010-05-20 Thread Bader Siddiki
Hello Laurie
Pi gives red color in IF, it stains DNA and RNA

PI excites at 535nmand emits at 615nm, producing a *red* fluorescence.

Therefore if you are using any chromophore at these wave lengths which emits
red color you will have problems.

By the way we also have several mounting mediums for IHC and IF, if you
would like to try, please let us know.

Bader
**



-- 
If any Q's please feel free to contact us
Have a nice day/weekend
Mit freundlichen Grüßen / With Kind Regards /
avec l'aimable ce qui concerne
Met vriendelijke groeten
種とについて
Bader
Bader B Siddiki, PhD
Executive director,
Research and development
ImmunoBioScience corp.
Phone: + 425 367 4601
Phone: + 425 514 3761
Fax: + 425 367 4817
cell (mobile) phone: + 425 314 0199
e-mail address: bade...@gmail.com
Web site: www.immunobioscience.com
Marketing: phone: + 650 343 IBSC (4272)
   E-mail: anitai...@aol.com
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RE: [Histonet] Mounting Medium for Immunofluorescence

2010-05-20 Thread Merced M Leiker
Actually if I could make a minor correction to your statement: propidium 
iodide is excited by green light at 488nm but emits in the red portion of 
the spectrum (620nm)...



--On Thursday, May 20, 2010 12:48 PM -0600 Liz Chlipala 
 wrote:



I think that the propidium iodide will stain the nuclei or DNA, similar
to DAPI, so this mounting media will counterstain the nuclei and its
visible in the FITC wavelength (488), while if you conterstain with DAPI
it is excited with a different wavelength, therefore it might interfere
with the FITC portion of your stain.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, Colorado 80308
office (303) 682-3949
fax (303) 682-9060
www.premierlab.com


Ship to Address:
1567 Skyway Drive, Unit E
Longmont, Colorado 80504

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Laurie
Colbert
Sent: Thursday, May 20, 2010 12:13 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Mounting Medium for Immunofluorescence

We normally coverslip our immunofluorescence slides with a permanent
aqueous mounting medium.  By mistake, today they were coverslipped with
a mounting medium that we use for coverslipping our FISH slides.  This
mounting medium is described as "Mounting medium for fluorescence, with
propidium iodide."

Will this make a difference when reading the slides??



Laurie Colbert

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Merced M Leiker
Research Technician III
Cardiovascular Medicine
348 Biomedical Research Building
State University of New York at Buffalo
3435 Main St, Buffalo, NY 14214  USA
lei...@buffalo.edu
716-829-6118 (Ph)
716-829-2665 (Fx)

No trees were harmed in the sending of this email.
However, many electrons were severely inconvenienced.


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Re: [Histonet] Mounting Medium for Immunofluorescence

2010-05-20 Thread Merced M Leiker

Hi Laurie,

Propidium iodide is a DNA intercalator that fluoresces red (ex 488nm).

Regards,
Merced

--On Thursday, May 20, 2010 11:13 AM -0700 Laurie Colbert 
 wrote:



We normally coverslip our immunofluorescence slides with a permanent
aqueous mounting medium.  By mistake, today they were coverslipped with
a mounting medium that we use for coverslipping our FISH slides.  This
mounting medium is described as "Mounting medium for fluorescence, with
propidium iodide."

Will this make a difference when reading the slides??



Laurie Colbert

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Merced M Leiker
Research Technician III
Cardiovascular Medicine
348 Biomedical Research Building
State University of New York at Buffalo
3435 Main St, Buffalo, NY 14214  USA
lei...@buffalo.edu
716-829-6118 (Ph)
716-829-2665 (Fx)

No trees were harmed in the sending of this email.
However, many electrons were severely inconvenienced.


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RE: [Histonet] Mounting Medium for Immunofluorescence

2010-05-20 Thread Liz Chlipala
I think that the propidium iodide will stain the nuclei or DNA, similar
to DAPI, so this mounting media will counterstain the nuclei and its
visible in the FITC wavelength (488), while if you conterstain with DAPI
it is excited with a different wavelength, therefore it might interfere
with the FITC portion of your stain.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, Colorado 80308
office (303) 682-3949 
fax (303) 682-9060
www.premierlab.com
 
 
Ship to Address:
1567 Skyway Drive, Unit E
Longmont, Colorado 80504

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Laurie
Colbert
Sent: Thursday, May 20, 2010 12:13 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Mounting Medium for Immunofluorescence

We normally coverslip our immunofluorescence slides with a permanent
aqueous mounting medium.  By mistake, today they were coverslipped with
a mounting medium that we use for coverslipping our FISH slides.  This
mounting medium is described as "Mounting medium for fluorescence, with
propidium iodide."

Will this make a difference when reading the slides??

 

Laurie Colbert

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[Histonet] Mounting Medium for Immunofluorescence

2010-05-20 Thread Laurie Colbert
We normally coverslip our immunofluorescence slides with a permanent
aqueous mounting medium.  By mistake, today they were coverslipped with
a mounting medium that we use for coverslipping our FISH slides.  This
mounting medium is described as "Mounting medium for fluorescence, with
propidium iodide."

Will this make a difference when reading the slides??

 

Laurie Colbert

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Re: [Histonet] Mounting Medium Issues

2010-05-18 Thread Jack Ratliff

Jim,

I must say that I have used Eukitt exclusively for over 13 years and  
the only problem I have had with Eukitt is when I do not use enough  
and especially when coverslipping thicker sections. It is xylenes  
based so maybe you are experiencing excessive evaporation during  
drying??? If you are diluting the Eukitt with xylenes so that it flows  
better (not as viscious) and reduces the chance for air bubbles, this  
might cause excessive shrinkage and/or the problems you are  
experiencing.


Jack

On May 14, 2010, at 3:22 PM, "Herrick, James L. (Jim)" > wrote:



Hello everyone,

Hope you have all had a good week!!

Has anyone had any negative experiences using Eukitt as your mounting
media? We embed our specimens (human - iliac crest, animal - femurs,
tibias, vertebrae, etc.) in GMA and MMA and have noticed over a period
of time that a fairly large number of our slides are beginning to show
signs of the medium breaking up below the cover slip. It looks as if
there are large air pockets left behind. We are not yet sure why  
this is
happening. Would any of you geniuses have a resolution for us or  
know of

a better mounting media to use with plastic embedded specimens -
brightfield and fluorescence? Thanks for your expertise. It is very  
much

appreciated.

Have a great weekend!!

Jim


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FW: [Histonet] Mounting Medium Issues

2010-05-17 Thread Herrick, James L. (Jim)


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Herrick,
James L. (Jim)
Sent: Friday, May 14, 2010 3:23 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Mounting Medium Issues

Hello everyone,

Hope you have all had a good week!!

Has anyone had any negative experiences using Eukitt as your mounting
media? We embed our specimens (human - iliac crest, animal - femurs,
tibias, vertebrae, etc.) in GMA and MMA and have noticed over a period
of time that a fairly large number of our slides are beginning to show
signs of the medium breaking up below the cover slip. It looks as if
there are large air pockets left behind. We are not yet sure why this is
happening. Would any of you geniuses have a resolution for us or know of
a better mounting media to use with plastic embedded specimens -
brightfield and fluorescence? Thanks for your expertise. It is very much
appreciated.

Have a great weekend!!

Jim


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[Histonet] Mounting Medium Issues

2010-05-14 Thread Herrick, James L. (Jim)
Hello everyone,

Hope you have all had a good week!!

Has anyone had any negative experiences using Eukitt as your mounting
media? We embed our specimens (human - iliac crest, animal - femurs,
tibias, vertebrae, etc.) in GMA and MMA and have noticed over a period
of time that a fairly large number of our slides are beginning to show
signs of the medium breaking up below the cover slip. It looks as if
there are large air pockets left behind. We are not yet sure why this is
happening. Would any of you geniuses have a resolution for us or know of
a better mounting media to use with plastic embedded specimens -
brightfield and fluorescence? Thanks for your expertise. It is very much
appreciated.

Have a great weekend!!

Jim


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