[ccp4bb] intersection curve of cone with cylinder

2008-03-19 Thread Bernhard Rupp
Dear All - if memory serves me right, the intersection curve of a cone with a cylinder (like in a powder diffraction camera) is not an ellipse, but a "Schnittfigur zweiter Ordnung", loosly translated as 'intersection curve of second order'. Is there a correct English term for that type of curve?

[ccp4bb] Back-Fourier transforming an EM map with SFALL

2008-03-19 Thread Lawrence Shapiro
Hello All, I'm trying to back-FT an EM map to produce Fs and phases to use in molecular replacement, but am having a problem with running SFALL. Here's the issue: When running the back-transform in SFALL (generating structure factors from a map), I get the following error: Incorrect sampling gri

Re: [ccp4bb] The Lawnmower Man

2008-03-19 Thread James Stroud
Neural lace embedding is too barbaric. Has anyone written a coot driver for this non-invasive alternative: http://techreport.com/discussions.x/13928 ? The superiority of thought driven building over dragging a mouse + modifier keys might be reason enough to get me to switch from O. Jame

Re: [ccp4bb] PRODRG?

2008-03-19 Thread Winn, MD (Martyn)
Yes, it is down at the moment, due to a hacking event apparently. Don't know any more. Martyn -Original Message- From: CCP4 bulletin board on behalf of Harry M. Greenblatt Sent: Wed 3/19/2008 1:28 PM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] PRODRG? BS"D Hi all, Does anybody know

Re: [ccp4bb] Crystal scoring sheets for iPhones/Pocket-PCs

2008-03-19 Thread P Hubbard
Toys for crystallography: Maybe this could be filed under the same category as Johnny Chung Lee's Wii Remote projects a number of people here have mentioned about (though this certainly isn’t as cool as his “Minority Report” gloves or "fake" 3D-glasses)! Still, if Apple has it's way, in a year

Re: [ccp4bb] Phased Anomalous Difference Map

2008-03-19 Thread Kay Diederichs
Rajan Pillai schrieb: Hi All, Can anyone tell me how I calculate a phased anomalous difference map? I want to confirm such as the presence of Cl or S ion in an unknown electron density. Rajan. use coot and select "expert" for specifying labels - it gives you a choice for a anom diff map.

[ccp4bb] Phased Anomalous Difference Map

2008-03-19 Thread Rajan Pillai
Hi All, Can anyone tell me how I calculate a phased anomalous difference map? I want to confirm such as the presence of Cl or S ion in an unknown electron density. Rajan.

Re: [ccp4bb] Crystal scoring sheets for iPhones/Pocket-PCs

2008-03-19 Thread William Scott
Sounds great. You can jailbreak an iPhone/iPod touch and install a whole BSD subsystem on it. So printing should be possible at some point... This of course also suggests that iPod touch is a valid research expense ... William G. Scott Contact info: http://chemistry.ucsc.edu/~wgscott/

[ccp4bb] Senior Scientist Position at Evotec

2008-03-19 Thread Mather, Owen
Apologies for the slightly off topic post but can I draw people's attention to the job advert below. Due to continued expansion Evotec has a post for a Senior Scientist to join the X-ray crystallography team based in Abingdon, UK. Working within a small multidisciplinary project team your task wi

Re: [ccp4bb] cryoprotectant for crystals in isopropanol

2008-03-19 Thread Radisky, Evette S., Ph.D.
Another anecdote for you: I had crystals that grew in 15% PEG 2000 and 15% isopropanol. Cryos with glycerol melted the crystals. The best cryo I found was with 15% PEG 2000, 15% isopropanol, and 20% PEG 400. It's hard to predict how your crystals will behave with different cryoprotectants-- hopef

[ccp4bb] Crystal scoring sheets for iPhones/Pocket-PCs

2008-03-19 Thread P Hubbard
Hi, I could be wrong, but I’m not aware of any servers that have tray inspection web pages designed for iPhones and Pocket-PCs, so I thought I’d try and make a web page with simple scoring sheets for such devices. I don’t own an iPhone myself, but the following web page seems to work well on a

[ccp4bb] Workshop on Raw Image Formats in Structural Biology

2008-03-19 Thread Herbert J. Bernstein
There will be a workshop on Raw Image Formats in Structural Biology to be held at Brookhaven National Laboratory on 22 May 2008. Over the past 2 years, imgCIF has seen increasing use, and the interactions among raw image formats for x-ray crystallography, neutron crystallography and microscopy ha

[ccp4bb] PRODRG?

2008-03-19 Thread Harry M. Greenblatt
BS"D Hi all, Does anybody know what happened to the PRODRG server web site? Thanks Harry - Harry M. Greenblatt Staff Scientist Dept of Structural Biology [EMAIL PROTECTED] Weizmann Institute of Science

Re: [ccp4bb] cryoprotectant for crystals in isopropanol

2008-03-19 Thread David Roberts
Not that all proteins are created alike, but we had a protein crystallize in a similar situation (PEG/20%IPA). I don't remember the exact details, but we did have good luck using just glycerol as a cryoprotectant. That is, I made up the well buffer and added to that well buffer 20-25% glycer

[ccp4bb] Post-Doctoral position at the University of Dundee

2008-03-19 Thread Paul Fyfe
UNIVERSITY OF DUNDEE COLLEGE OF LIFE SCIENCES DIVISION OF BIOLOGICAL CHEMISTRY AND DRUG DISCOVERY POSTDOCTORAL RESEARCH ASSISTANT GRADE 7 (£26,666 - £32,796) Enthusiastic, highly motivated individuals are sought to study key enzymes of microbial pathogens to advance fundamental understanding of

Re: [ccp4bb] The Lawnmower Man

2008-03-19 Thread David Briggs
Wow, that is like, so last week(!). I'm using COOT though a wireless interface to a head-up-display mounted inside my contact lenses, controlled via a neural lace* embedded directly into my parietal lobe. I can refine and rebuild my structure whilst doing minipreps, having a coffee, in the shower

[ccp4bb] The Lawnmower Man

2008-03-19 Thread Jordi Benach
Dear everyone, Has anybody tried to hook up a protein display/model building program/computer with 3D glasses of the type people are developing for games? The type of glasses where one obviates the use of large monitors altogether, that is the display sits on the glasses themselves. Thanks,

Re: [ccp4bb] Broken Masks

2008-03-19 Thread Laurence Pearl
I think it is a version bug of some sort - when I ran the same mask creation run in CCP4i on my MacBook laptop (which has the most recent CCP4) it displays fine and also worked perfectly in dm averaging, turning a tangled mess into interpretable density. The mask generated by NCSMASK on my des

[ccp4bb] cryoprotectant for crystals in isopropanol

2008-03-19 Thread shivesh kumar
Dear all, The question is regarding the cryoprotectant.We have crystallized a protein in 20% PEG 500 with 10% isopropanol.What should be the cryo we should try for data collection.Also,what percentage of PEG 500 is sufficient enough as a cryo for data collection. We are also trying to crystallize i