Re: [ccp4bb] neutron data measurement

gt; very limited on a molar basis within the crystal. As can be seen with > Benno’s myoglobin crystal, 30 years of iron activation has yet to produce a > measurable defect. > > > Leif Hanson > > University of Toledo > -- patr...@douglas.co.ukDouglas Instruments Ltd.

Re: [ccp4bb] Complex seeding

-- patr...@douglas.co.ukDouglas Instruments Ltd. Douglas House, East Garston, Hungerford, Berkshire, RG17 7HD, UK Directors: Peter Baldock, Patrick Shaw Stewart http://www.douglas.co.uk Tel: 44 (0) 148-864-9090US toll-free 1-877-225-2034 Regd. England 2177994, VAT Reg. GB 480 7371 36

Re: [ccp4bb] UV imaging of crystals

what a Rock UV Imager does. I know >> > this is probably a crazy dream. However, I would greatly appreciate >> > any comments, advice or experience any of you may have. >> > >> > Molecular Dimension do such an adaptor which fits to existing >> microscopes. >> > >> > See >> > < >> http://www.moleculardimensions.com/shopdisplayproducts.asp?id=121&cat=X%2DtaLight%3Csup%3E%99%3C%2Fsup%3E+100+%2D+UV+for+Microscope+ >> > >> > >> > >> > >> > This message was sent using IMP, the Internet Messaging Program. >> > >> > .. >> > Jürgen Bosch >> > Johns Hopkins University >> > Bloomberg School of Public Health >> > Department of Biochemistry & Molecular Biology >> > Johns Hopkins Malaria Research Institute >> > 615 North Wolfe Street, W8708 >> > Baltimore, MD 21205 >> > Office: +1-410-614-4742 >> > Lab: +1-410-614-4894 >> > Fax: +1-410-955-2926 >> > http://web.mac.com/bosch_lab/ >> > >> > >> > >> > >> > >> > >> > > -- patr...@douglas.co.ukDouglas Instruments Ltd. Douglas House, East Garston, Hungerford, Berkshire, RG17 7HD, UK Directors: Peter Baldock, Patrick Shaw Stewart http://www.douglas.co.uk Tel: 44 (0) 148-864-9090US toll-free 1-877-225-2034 Regd. England 2177994, VAT Reg. GB 480 7371 36

Re: [ccp4bb] Crystals with Organic solvents

; Thanks in Advance > > --  patr...@douglas.co.uk    Douglas Instruments Ltd.  Douglas House, East Garston, Hungerford, Berkshire, RG17 7HD, UK  Directors: Peter Baldock, Patrick Shaw Stewart  http://www.douglas.co.uk  Tel: 44 (0) 148-864-9090    US toll-free 1-877-225-2034  Regd. England 2177994, VAT Reg. GB 480 7371 36

Re: [ccp4bb] Fab:antigen complex crystallization!!!

e most common precipitant for a particular class of proteins > is better spent on setting up more trays. > > Cheers, > > Ed. > > -- > Oh, suddenly throwing a giraffe into a volcano to make water is crazy? >         Julian, King of

Re: [ccp4bb] Fab:antigen complex crystallization!!!

I would really appreciate any suggestion,help, direction. > Thanks > ivan -- patr...@douglas.co.ukDouglas Instruments Ltd. DouglasHouse, EastGarston, Hungerford, Berkshire, RG177HD, UK Directors: Peter Baldock, Patrick Shaw Stewart http://www.douglas.co.uk Tel: 44 (0) 148-864-9090

Re: [ccp4bb] small lysozyme crystals?

m is > 100 um wide. > > I'm sure someone has done this before? > > -James Holton > MAD Scientist > --  patr...@douglas.co.uk    Douglas Instruments Ltd.  DouglasHouse, EastGarston, Hungerford, Berkshire, RG177HD, UK  Directors: Peter Baldock, Patrick Shaw Stewart  http://

Re: [ccp4bb] need proper suggestion

as Instruments Ltd. DouglasHouse, EastGarston, Hungerford, Berkshire, RG177HD, UK Directors: Peter Baldock, Patrick Shaw Stewart http://www.douglas.co.uk Tel: 44 (0) 148-864-9090US toll-free 1-877-225-2034 Regd. England 2177994, VAT Reg. GB 480 7371 36

Re: [ccp4bb] Reproducing crystals.

experience like this? Is PEG4000 degraded or oxidized? > > Please help me. > > Thanks in advance. > -- patr...@douglas.co.ukDouglas Instruments Ltd. DouglasHouse, EastGarston, Hungerford, Berkshire, RG177HD, UK Directors: Peter Baldock, Patrick Shaw Stewart http:/

Re: [ccp4bb] crystallizing a complex that's sensitive to ionic strength

arston, Hungerford, Berkshire, RG177HD, UK Directors: Peter Baldock, Patrick Shaw Stewart http://www.douglas.co.uk Tel: 44 (0) 148-864-9090US toll-free 1-877-225-2034 Regd. England 2177994, VAT Reg. GB 480 7371 36

Re: [ccp4bb] Your suggestions needed: Difficulties in reproducing HT crystallization conditions. Thanks!

discussion about protein crystallization and automation, please join our bulletin board at http://groups-beta.google.com/group/oryx_group?hl=en patr...@douglas.co.ukDouglas Instruments Ltd. DouglasHouse, EastGarston, Hungerford, Berkshire, RG177HD, UK Directors: Peter Baldock, P

[ccp4bb] Fab purification and crystallization

-beta.google.com/group/oryx_group?hl=en patr...@douglas.co.ukDouglas Instruments Ltd. DouglasHouse, EastGarston, Hungerford, Berkshire, RG177HD, UK Directors: Peter Baldock, Patrick Shaw Stewart http://www.douglas.co.uk/ Tel: 44 (0) 148-864-9090US toll-free 1-877-225-2034 Regd

Re: [ccp4bb] turn granular to crystal

uglasHouse, EastGarston, Hungerford, Berkshire, RG177HD, UK Directors: Peter Baldock, Patrick Shaw Stewart http://www.douglas.co.uk/ Tel: 44 (0) 148-864-9090US toll-free 1-877-225-2034 Regd. England 2177994, VAT Reg. GB 480 7371 36 From: CCP4 bulletin board [mailto:ccp...@jiscmail.ac.u

Re: [ccp4bb] Scaling up from an Intelliplate to Linbro Plate

rd at http://groups-beta.google.com/group/oryx_group?hl=en patr...@douglas.co.ukDouglas Instruments Ltd. DouglasHouse, EastGarston, Hungerford, Berkshire, RG177HD, UK Directors: Peter Baldock, Patrick Shaw Stewart http://www.douglas.co.uk/ Tel: 44 (0) 148-864-9090US toll-free 1-87

Re: [ccp4bb] control of nucleation

irectors: Peter Baldock, Patrick Shaw Stewart http://www.douglas.co.uk/ Tel: 44 (0) 148-864-9090US toll-free 1-877-225-2034 Regd. England 2177994, VAT Reg. GB 480 7371 36 From: CCP4 bulletin board [mailto:ccp...@jiscmail.ac.uk] On Behalf Of zq deng Sent: 06 May 2010 09:04 To: CCP4BB

Re: [ccp4bb] freezing crystals grown in isopropanol condition

; Can isopropanol itself act as a cryoprotectant? > Any suggestions on how to deal with isopropanol evaporation during > mounting? > > Many thanks and best wishes, > Chris > > > -- patr...@douglas.co.ukDouglas Instruments Ltd. DouglasHouse, EastGarston, Hungerford, Berk

Re: [ccp4bb] 24-well sitting-drop plates

e, EastGarston, Hungerford, Berkshire, RG177HD, UK > Directors: Peter Baldock, Patrick Shaw Stewart > http://www.douglas.co.uk/ > Tel: 44 (0) 148-864-9090US toll-free 1-877-225-2034 > Regd. England 2177994, VAT Reg. GB 480 7371 36 > > > -Original Message- > > Fro

Re: [ccp4bb] 24-well sitting-drop plates

patr...@douglas.co.ukDouglas Instruments Ltd. DouglasHouse, EastGarston, Hungerford, Berkshire, RG177HD, UK Directors: Peter Baldock, Patrick Shaw Stewart http://www.douglas.co.uk/ Tel: 44 (0) 148-864-9090US toll-free 1-877-225-2034 Regd. England 2177994, VAT Reg. GB 480 7371 36

[ccp4bb] Vapor diffusion calculator

ouse, EastGarston, Hungerford, Berkshire, RG177HD, UK Directors: Peter Baldock, Patrick Shaw Stewart http://www.douglas.co.uk/ Tel: 44 (0) 148-864-9090US toll-free 1-877-225-2034 Regd. England 2177994, VAT Reg. GB 480 7371 36 > -Original Message- > From: CCP4 bulletin

Re: [ccp4bb] stabilizing solution

//groups-beta.google.com/group/oryx_group?hl=en patr...@douglas.co.ukDouglas Instruments Ltd. DouglasHouse, EastGarston, Hungerford, Berkshire, RG177HD, UK Directors: Peter Baldock, Patrick Shaw Stewart http://www.douglas.co.uk/ Tel: 44 (0) 148-864-9090US toll-free 1-877-225-2034 Regd

Re: [ccp4bb] anaerobic crystallization

roup?hl=en patr...@douglas.co.ukDouglas Instruments Ltd. DouglasHouse, EastGarston, Hungerford, Berkshire, RG177HD, UK Directors: Peter Baldock, Patrick Shaw Stewart http://www.douglas.co.uk/ Tel: 44 (0) 148-864-9090US toll-free 1-877-225-2034 Regd. England 2177994, VAT Reg. GB 4

Re: [ccp4bb] Seeding

patr...@douglas.co.uk <mailto:patr...@douglas.co.uk> Douglas Instruments Ltd. DouglasHouse, EastGarston, Hungerford, Berkshire, RG177HD, UK Directors: Peter Baldock, Patrick Shaw Stewart http://www.douglas.co.uk/ <http://www.douglas.co.uk/> Tel: 44 (0) 148-864-9090US toll-fr

Re: [ccp4bb] How to improve crystal which is twinning?

ruments Ltd. DouglasHouse, EastGarston, Hungerford, Berkshire, RG177HD, UK Directors: Peter Baldock, Patrick Shaw Stewart http://www.douglas.co.uk/ <http://www.douglas.co.uk/> Tel: 44 (0) 148-864-9090US toll-free 1-877-225-2034 Regd. England 2177994, VAT Reg. GB 480 7371 36 From: CC

Re: [ccp4bb] Experimental design and response surface methods for crystal optimization

uming! There's more info at http://www.douglas.co.uk/rat_des.htm Patrick --  patr...@douglas.co.uk    Douglas Instruments Ltd.  DouglasHouse, EastGarston, Hungerford, Berkshire, RG177HD, UK  Directors: Peter Baldock, Patrick Shaw Stewart  http://www.douglas.co.uk/  Tel: 44 (0) 148-864-9090 

Re: [ccp4bb] microbatch vs hanging drop

patr...@douglas.co.ukDouglas Instruments Ltd. DouglasHouse, EastGarston, Hungerford, Berkshire, RG177HD, UK Directors: Peter Baldock, Patrick Shaw Stewart http://www.douglas.co.uk/ Tel: 44 (0) 148-864-9090US toll-free 1-877-225-2034 Regd. England 2177994, VAT Reg. GB 480 737

Re: [ccp4bb] Crystal vacuum cleaner

ouse, EastGarston, Hungerford, Berkshire, RG177HD, UK Directors: Peter Baldock, Patrick Shaw Stewart http://www.douglas.co.uk/ <http://www.douglas.co.uk/> Tel: 44 (0) 148-864-9090US toll-free 1-877-225-2034 Regd. England 2177994, VAT Reg. GB 480 7371 36 From: CCP4 bul

Re: [ccp4bb] Crystallography plates, hanging drop but templated sealing film.

, please join our bulletin board at http://groups.google.com/group/oryx_group/members_invite?hl=en patr...@douglas.co.ukDouglas Instruments Ltd. DouglasHouse, EastGarston, Hungerford, Berkshire, RG177HD, UK Directors: Peter Baldock, Patrick Shaw Stewart http://www.douglas.co.uk/ Tel: 44 (0) 148

Re: [ccp4bb] Query on program for creating random crystallization screen

DouglasHouse, EastGarston, Hungerford, Berkshire, RG177HD, UK Directors: Peter Baldock, Patrick Shaw Stewart http://www.douglas.co.uk/ Tel: 44 (0) 148-864-9090US toll-free 1-877-225-2034 Regd. England 2177994, VAT Reg. GB 480 7371 36 > -Original Message- > From: CCP4 bulletin boa

Re: [ccp4bb] about microbatch

rston, Hungerford, Berkshire, RG177HD, UK Directors: Peter Baldock, Patrick Shaw Stewart http://www.douglas.co.uk/ Tel: 44 (0) 148-864-9090US toll-free 1-877-225-2034 Regd. England 2177994, VAT Reg. GB 480 7371 36 From: CCP4 bulletin board [mailto:[EMAIL PROTECTED] On Behalf Of Jiamu Du Sen

[ccp4bb] crystallisation robot

rford, Berkshire, RG177HD, UK Directors: Peter Baldock, Patrick Shaw Stewart, James Smith http://www.douglas.co.uk/ Tel: 44 (0) 148-864-9090US toll-free 1-877-225-2034 Regd. England 2177994, VAT Reg. GB 480 7371 36 From: CCP4 bulletin board [mailto:[EMAIL PROTECTED] On Behalf Of JOE CR

Re: [ccp4bb] isopropanol as a precipitant

Please let me know if anyone wants samples. Patrick -- [EMAIL PROTECTED]Douglas Instruments Ltd. DouglasHouse, EastGarston, Hungerford, Berkshire, RG177HD, UK Directors: Peter Baldock, Patrick Shaw Stewart, James Smith http://www.douglas.co.uk/ Tel: 44 (0) 148-864-9090

[ccp4bb] Fwd: [ccp4bb] crystallisation robot

One thing that people often overlook is that quite a lot of protein can be lost by denaturation on the surface of the drop. This is more significant for smaller drops. Two suggestions: (1) increase the proportion of protein in the - technical term - teeny drop to say two thirds and (2) cover the

Re: [ccp4bb] crystallization robot

voirs, but they can do optimization of the drops. Best wishes Patrick -- [EMAIL PROTECTED]Douglas Instruments Ltd. DouglasHouse, EastGarston, Hungerford, Berkshire, RG177HD, UK Directors: Peter Baldock, Patrick Shaw Stewart, James Smith http://douglas.co.uk or http://www.douglas.co.uk

Re: [ccp4bb] Off Topic:crystallization in the presence of glycerol

Rob, A couple of years ago my colleague and I "mined" the crystallization data in REMARK 280 from 3939 entries in the PDB. In 109 entries, glycerol came out as the ingredient with the highest concentration that was measured with a % (percent) sign i.e. including '%', '%W/V', '%V/V' etc. The aver

Re: [ccp4bb] Help with reducing crystal mosaicity

25.7%. You can see the data at www.douglas.co.uk/top14.htm I thought this information could be useful if you want to replace some of the MPD with another precipitant (or cryoprotectant). Best wishes Patrick Shaw Stewart --  [EMAIL PROTECTED] Douglas Instruments Ltd.  DouglasHouse

[ccp4bb] Demonstration and support specialist

"salesmen" please. Please reply to the address below, or e-mail. Patrick Shaw Stewart --  [EMAIL PROTECTED] Douglas Instruments Ltd.  DouglasHouse, EastGarston, Hungerford, Berkshire, RG177HD, UK Directors: Peter Baldock, Patrick Shaw Stewart, James Smith http://douglas.co.

Re: [ccp4bb] Main topic of the day: Protein crystallization

Ronaldo I have a database of crystallization conditions extracted from the PDB. I was able to parse the data to extract the concentration of protein in around 900 cases. The lowest 9 protein concentrations were as follows: PDB ID TYPEDATEProtein conc, mg/ml 1EYMISOMERASE.

Re: [ccp4bb] Question about glove boxes for protein crystallization

Hi Mathews One of our customers, Bret Dillard at the University of Georgia, has had great success with one of our robots in a Bactron X anaerobic chamber. In this case, Bret mainly used the microbatch-under-oil method, which works very well for anaerobic work: (1) the oil protects the sample an

Re: [ccp4bb] crystal friendly solvents that are useful for dissolving hydrophobic small molecules?

probably better) microbatch-under-oil. The oil acts as a reservoir that contains excess small molecule that (you hope) will be fed into the crystals. Patrick Shaw Stewart -- [EMAIL PROTECTED]Douglas Instruments Ltd. DouglasHouse, EastGarston, Hungerford, Berkshire, RG177HD, UK Directors

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