Joe
The Oryx robots take about 8 minutes to dispense 96 wells, plus about a minute to pick up protein etc. Evaporation is not an issue even for 100 + 100 nl drops, because there's an evaporation shield. This can include microseeding into screens (the D'Arcy "MMS" method) - which we now regard as an absolutely essential facility. Many robots can't do this because the tips get clogged. It can also include a simple 2-d optimization grid. Setup is very simple because you only have to put the protein (and seed stock) into one well. Experiments with three drops per reservoir take about 14 minutes. Additive experiments take longer - the tip has further to go. Patrick http://www.douglas.co.uk/mms.htm http://www.douglas.co.uk/products.htm -- [EMAIL PROTECTED] Douglas Instruments Ltd. DouglasHouse, EastGarston, Hungerford, Berkshire, RG177HD, UK Directors: Peter Baldock, Patrick Shaw Stewart, James Smith http://www.douglas.co.uk/ Tel: 44 (0) 148-864-9090 US toll-free 1-877-225-2034 Regd. England 2177994, VAT Reg. GB 480 7371 36 From: CCP4 bulletin board [mailto:[EMAIL PROTECTED] On Behalf Of JOE CRYSTAL Sent: 14 April 2008 22:10 To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] Fwd: [ccp4bb] crystallisation robot Hi, Does anyone have information about how long it takes to set up a 96-well tray for the crystallization robots available? Besides cost per tray and maintenance cost, another important feature we consider is the time for setting up a 96-well tray. It is an important factor since we are talking about sub-microliter drops. Best, Joe
