Order of addition when making the solutions is often important to dilute
the least soluble component rapidly. Zn acetate is the most soluble Zn
salt. Put that in your tube first and then dilute it with your largets
volume (probably either water) and then add the other ingredients, consider
vortex
gt;
>> On Fri, May 11, 2012 at 11:43 AM, Rajesh Kumar wrote:
>>
>>> If there is no cure , then fine.
>>> pH may not be the answer as it doesn't Happen with TRIS buffer pH 7.6.
>>> Thanks to every one
>>> Rajesh
>>>
>>>
ery one
Rajesh
Date: Fri, 11 May 2012 12:35:45 -0400
From: dj...@cornell.edu <mailto:dj...@cornell.edu>
Subject: Re: [ccp4bb] zinc with HEPES
To: CCP4BB@JISCMAIL.AC.UK <mailto:CCP4BB@J
ops with more than 100 tiny needles with a tough membrane on it
> has been frustrating as I end up loosing several drops to just to fish out
> 1-2 needles. I am ready to try if there any trick left.
>
> Thanks for lots and lots of help.
>
> Regards,
> Rajesh
>
Thanks. I will try.
Rajesh
Date: Fri, 11 May 2012 12:38:20 -0500
From: j-kell...@fsm.northwestern.edu
Subject: Re: [ccp4bb] zinc with HEPES/seeding
To: CCP4BB@JISCMAIL.AC.UK
mitegen loops might help, particularly micromesh...
JPK
On Fri, May 11, 2012 at 12:35 PM, Rajesh Kumar wrote:
Dear
n (yet to collect data) but manipulating these
> crystal drops with more than 100 tiny needles with a tough membrane on it
> has been frustrating as I end up loosing several drops to just to fish out
> 1-2 needles. I am ready to try if there any trick left.
>
> Thanks for lots and lots of
rds,Rajesh
Date: Fri, 11 May 2012 18:09:54 +0100
Subject: Re: [ccp4bb] zinc with HEPES
From: patr...@douglas.co.uk
To: ccp4...@hotmail.com
Rajesh
How did you do the MMS? By hand or with a robot, and what screens did you use?
and why did you change to HEPES out of interest?
Patrick
On 11 May 2012 18:
Almost assuredly you are getting zinc hydroxide precipitate. Zn(OH)2
will readily precipitate from solutions of alkaline Zn(II). Your problem
is compounded by the fact that HEPES is a non-coordinating buffer, so it
does not help solubilize the zinc ion. You might find a weakly
coordinating buff
not be the answer as it doesn't Happen with TRIS buffer pH 7.6.
>> Thanks to every one
>> Rajesh
>>
>>
>> --
>> Date: Fri, 11 May 2012 12:35:45 -0400
>> From: dj...@cornell.edu
>>
increase
thickness or change the shape of very very thin needle crystals.
Yes, I will try very less, 50uM.Thanks for helping me to understand.Rajesh
> Date: Fri, 11 May 2012 12:53:53 -0400
> Subject: Re: [ccp4bb] zinc with HEPES
> From: liehy...@gmail.com
> To: ccp4...@hotmail.com
pH may not be the answer as it doesn't Happen with TRIS buffer pH 7.6.
> Thanks to every one
> Rajesh
>
>
> --
> Date: Fri, 11 May 2012 12:35:45 -0400
> From: dj...@cornell.edu
>
> Subject: Re: [ccp4bb] zinc with HEPES
> To: CCP4BB@JIS
pH is the culprit here
Like some already mentioned
change your pH to 6.4 and use a different buffer like cacodylate
or you can use Zinc acetate in water pH still would be 6.4
from your last mail
why would you add Zn to your initial hit condition
is there any rationale?
Padayatti
On Fri, May 11,
If there is no cure , then fine.pH may not be the answer as it doesn't Happen
with TRIS buffer pH 7.6.Thanks to every oneRajesh
Date: Fri, 11 May 2012 12:35:45 -0400
From: dj...@cornell.edu
Subject: Re: [ccp4bb] zinc with HEPES
To: CCP4BB@JISCMAIL.AC.UK
There
many might have used
this combination.
ThanksRajesh
Date: Fri, 11 May 2012 11:29:03 -0500
Subject: Re: [ccp4bb] zinc with HEPES
From: j-kell...@fsm.northwestern.edu
To: ccp4...@hotmail.com
CC: CCP4BB@jiscmail.ac.uk
Try adding water first, so that you are not mixing concentrated Zn with
On 05/11/12 12:26, Rajesh Kumar wrote:
Dear All,
This question sounds simple but I dont know the answer.
I was preparing a 24 well crystal screen. When I try to use 10 mM
ZnSO4 with HEPES (pH 7.6) buffer it precipitates. I tried both ZnCl2
and Zn acetate the effect is same.
I dont know why t
-8-646-1710
From: CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] on behalf of Rajesh Kumar [ccp4...@hotmail.com]
Sent: Friday, May 11, 2012 7:26 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] zinc with HEPES
Dear All,
This question sounds simple but I dont know the
Try adding water first, so that you are not mixing concentrated Zn with
concentrated HEPES. Also it depends what else is in your cocktail.
JPK
On Fri, May 11, 2012 at 11:26 AM, Rajesh Kumar wrote:
> Dear All,
>
> This question sounds simple but I dont know the answer.
> I was preparing a 24 we
Dear All,
This question sounds simple but I dont know the answer.I was preparing a 24
well crystal screen. When I try to use 10 mM ZnSO4 with HEPES (pH 7.6) buffer
it precipitates. I tried both ZnCl2 and Zn acetate the effect is same. I dont
know why this Zn in not compatible with HEPES.Could
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