do a search on the histonet archive
i posted a method quite a few years back
processed and cut eyes (human and piggy) on a daily basis for almost 10
years
go find it - it may help
patience, determination, thicker sections, 2 x pre-float out first on dist
water and then very dilute alcohol to manua
Hi,
Does anyone out there know if there is a monoclonal anti human NK cell antibody
that is exclusive, ie does not also mark CD8 T cells etc?
Kim
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Re:
Hi Nancy, do Tuj1 (beta 3 tubilin) stain nicely in adult rat brain? I think
Tuj1 is restricted to immature neurons inside the brain ! though it stains well
with retinal ganglion cell
I am also seeking for a nice marker for axon ...both normal and
regenerating...GAP-43 is one choice for
An additional step that often is helpful is to submit pericolonic adipose
tissue with vessels... frequently you can find microscopic lymph nodes/lymphoid
aggregates.
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Doesn't help with the wrinkles in OLD large Histotechs.
Sorry, couldn't resist
Regards
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Stree
Hi All,
Does anybody have an owner's manual to an Autotechnicon 2A? I've inherited
this 42 year old beasty and can't remember how to work the delay timer.
Yes, I am old enough to have used one of these R2D2 units in the past.
If you can help me, I'd be much obliged.
Paula :-)
Paula Sicurel
Hi Vanessa,
I have read in at least one online protocol and have also been advised by
our excellent and experienced (former) histotech who used to be here at UB
NOT to warm the slides without air-drying first. We typically air-dry the
slides, yes, standing up, overnight, before warming them.
If I am not mistaken vectamount is an aqueous mountant, dpx is
xylene/toluene based and requires going thru alcohols and xylene to mount.
One thing I do and you can try it, is airdry the slides and the use
permanent mounting media without going thru alcohols and xylene, keep in
mind there is a litt
My suggestion would be to follow the procedure of the old ocular guru
from the AFIP, Peter Emanuale. He taught me how the section human and
rabbit eyes. It involves using 2 water baths, one of which is at room
temp... and you should not use charged/coated slides.
Check our his chapter on ocular hi
Hi everyone,
Quick question, I am finding after cutting a lot of slides for H&Es onto VWR
Superfrost Plus slides (these are the only slides we have at the moment)
that when I lift sections from the water bath ( of distilled water) a water
bubble stays under the sections on the slide. When I lie th
Good question! I'd like to know that, too! But with any size specimen,
please!
--On Wednesday, March 04, 2009 1:23 PM -0800 Va Paula Sicurello
wrote:
Really? Liquid laundry soap? Does this help with all wrinkles in large
specimens?
Paula Sicurello
VA Medical Center San Diego
Veterans M
Yes, really with any type of large (and small) sections. The liquid soap will
reduce the surface tension of water (more than heat) allowing the sections to
expand easily. Try it..
René J.
--- On Wed, 3/4/09, Va Paula Sicurello wrote:
From: Va Paula Sicurello
Subject: Re: [Histonet] problem ey
Really? Liquid laundry soap? Does this help with all wrinkles in large
specimens?
Paula Sicurello
VA Medical Center San Diego
Veterans Medical Research Foundation (VMRF)
Core Microscope Facility, room B141
3350 La Jolla Village Dr., MC151
San Diego, CA 92161
858-552-8585 x2397
--- On Wed,
Add a few drops (4-5) of liquid detergent, but not dishwasher detergent
(because it will dissolve the paraffin).
rené J.
--- On Wed, 3/4/09, Perry, Margaret wrote:
From: Perry, Margaret
Subject: [Histonet] problem eyes
To: "histonet@lists.utsouthwestern.edu"
Date: Wednesday, March 4, 2009, 4:
Please help! We have been trying to cut whole eyes from a pig. They need to
be nice enough for a publication. We are having the devil of a time because no
matter what we do there are wrinkles. If we turn the waterbath up to stretch
things out the retina detaches. Do any of you have suggesti
As many of you may or may not know, the Histobath countertop freezing bath has
been discontinued by Thermo (formerly known as Thermo-Shandon, Shandon-Lipshaw,
etc.). I was told that the freon that is used to allow the unit to cool to -60
is no longer allowed by the EPA to be used in ultra low refri
hello everyone,
i have a problem which i dont know how to tackle, the
problem is that i have purchased vector blue as a chromogen and it is xylene
soluble can anyone suggest me how to avoid this as funds are low i cant
purchase xylene substitutes.
second question b
Hello histonetters,
Would you teach me what is best stain for elastic fiber in rat lung except
weigert's stain?
I want to look at arteries.
Thanks in advance,
Weihua
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If there are not 12 to be found, document what you did, how you did it, and how
many you found.
You cannot find what is not there, but make sure you tried your best.
René J.
--- On Wed, 3/4/09, Smith Wanda wrote:
From: Smith Wanda
Subject: [Histonet] Lymph Nodes on Colon Cancers
To: "histonet@
Good Morning,
What is everyone doing to meet the recommendation from the American College of
Surgeons and American Cancer Society regarding finding a minimum of 12 lymph
nodes on colon cancer cases? Especially when there are not 12 lymph nodes to
be found???
Thanks,
Wanda
WANDA G. SMITH, HTL(A
Good Morning Victoria
I believe Market Lab carries plastic cassette holders.
Karen J Kay, MLT
Pathology Supervisor
Chinook Health Laboratory
Chinook Regional Hospital
960 - 19 st South
Lethbridge, Alberta, Canada
T1J 1W5
(403) 388 - 6061 (Phone)
(403) 388 - 6067 (Fax)
Message: 6
Date: Tue, 3
Hi,
I have some mouse hindlimbs that have catheterized with Omnipaque. The
Omnipaque contains 302 ml of iohexol which is equivalent to 140 mg of
organic iodine per ml. The mice will be sacrificed immediately after
being radiographed. They would like them to be processed in paraffin. I
am wonderin
Hello histonetters,
I have the following, in perfect working condition, items for sale:
tissue processor VIP1000 benchtop, just refurbished
Shandon 24-3 slide stainer
Surgipath PC3001, PC3002 embedding center
ducktless hood with new carbon filters
hood the a super quite vent syste
Try some freshly prepared cresyl violet solutions?
2009-03-04
TF
发件人： Walters, Katherine S
发送时间： 2009-03-04 04:09:07
收件人： histo...@pathology.swmed.edu
抄送：
主题： [Histonet] Cresyl violet problems
To the "brainy" histo-people,
I have a student doing a cresyl violet stain on some frozen
Does anyone know a good anti-cholin acetyltransferase (ChAT) antibody for
mouse spinal cord to do immunohistochemistry (frozen section)? I see a
couple of vendors with more than a couple of clon names.
Moreover, somebody recommended this neurotransmitter as a motor neuronal
marker. If you can give
How is everyone getting the water? We have a distiller in the lab but it will
need to be replaced soon. The question is another distiller or buy bottled??
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it may be worth your while to buy another distiller...bottled water will
cost more over time...
--On Wednesday, March 04, 2009 6:37 AM -0800 kristen arvidson
wrote:
How is everyone getting the water? We have a distiller in the lab but it
will need to be replaced soon. The question is anot
I would by the VIP6 with total confidence.
René J.
--- On Wed, 3/4/09, Farnham, Lori wrote:
From: Farnham, Lori
Subject: [Histonet] We are buying a new Tissue Processor and need imput
To: histonet@lists.utsouthwestern.edu
Date: Wednesday, March 4, 2009, 9:11 AM
Hi All,
We are a small Patholog
I agree with Tony that a -70 freezer is ideal. We wrap our samples in aluminum
foil and then place in a plastic container. If you only have access to a -20
freezer, it's helpful to place cold packs in the freezer, near where you will
store the muscle samples. When a -20 freezer cycles, the tempe
Hi All,
We are a small Pathology Lab which processes a little over 7000 surgical
cases a year. We have a mixed variety of specimens (GI, GYN, skins,
etc).
We have been approved to buy a new tissue processor and are looking at
the Shandon Pathcentre and the Tissue Tek VIP 6. Any imput about these
p
What markers and specific clones do you need help with specifically? Also mouse
testis really needs a PFA fix. Even if you do fresh frozen sections I suggest a
short (10 min) fix in PFA rather than acetone.
-Original Message-
From: "Marti Gaudes, Merce"
Date: Wed, 04 Mar 2009 09:51:39
Hello!
Can anyone help us with CD markers? We want to use some CD markers on mouse
testis, and the antibodies data sheets explain that only freeze samples,
cryosectioned and post-fixed with acetone can be used. We tried to do this
protocols, but without any result. We frozen the sample with
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