Lots of waste!
Lee Ann Baldridge
IUSM
Indpls.,IN
From: histonet-boun...@lists.utsouthwestern.edu
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V
[hor...@archildrens.org]
Sent: Friday, August 21, 2009 3:49 PM
To:
With the shortage of PCP controls what are you using as a possible
alternative?
Any information will be greatly appreciated.
Thank you.
Lin S. Bustamante, B.Sc.; HT(ASCP)
Research Associate
Histology Lab. Supervisor
Dept. of Veterinary Integrative Biosciences
Texas AM University
College Station,
Our home made recipe is : 1925 mls of 95% alcohol + 360 mls DH20 + 250 mls of
Formaldehyde + 125 mls Glacial Acetic Acid. Got this 20 years ago from one of
my pathologists' journals and it works really well. Let sit overnight and even
the smallest nodes will turn opaque against a somewhat
Can anyone give feedback on reagents/procedures to use for immunofluorescence
to reduce/eliminate red cell autofluorescence on FFPE sections. I am using an
indirect method of labeling for two antibodies (FITC and Texas Red).
I have not tried 0.1% sodium borohydride. Will this help for
We have been utilizing 0.25% Ammonium Hydroxide(28%) + 70% Ethanol v/v for 1
hour followed by 10 minutes in 50% Ethanol rinsing in several changes of
buffer afterward and it seems to be working well for a multitude of
autofluorescent problems on FFPE sections for us. We tried to Sodium
Hello all,
I am working in an Allergy and Immunology research lab and we have been trying
to work up a flow cytometry antibody for IHC on paraffin. The antibody is mouse
anti-human TCR V alpha24-Biotin from BeckmanCoulter. Does anyone have a working
procedure for this antibody? I have tried
Good Morning/Afternoon Histonetters,
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The copper binds with the hemoglobin in the RBC and greatly reduces or
eliminates autofluoresence depending on timing.
10 mM Copper Sulfate
10 mM Copper Sulfate
Cupric Sulfate...1.25 gm
50 mM Amonium acetate (pH5)500.0 ml
Adjust ph to 5.0 with 1.0 M NaOH
50 mM Ammonium
I wonder if 'storing these reagents in the refrigerator' concept originated
with the idea of 'keeping them in the dark'.
Janet
From: histonet-boun...@lists.utsouthwestern.edu on behalf of John Kiernan
Sent: Fri 8/21/2009 5:00 PM
To: Cheri Miller
Cc:
Folks,
It's my annual inquiry as to whether someone has optimized
immunohistochemistry for CD4 and CD8 on formalin-fixed paraffin mouse
tissue. Over the years we have tried lots of different antibodies but I
am wondering if there is anything new that we have not tried. Any leads
would be great!
When this happens in our lab, we put Drierite (anhydrous calcium
sulfate) into the clearing agents. Just put enough to have not quite a
solid layer on the bottom of the staining well, so it will not block the
staining rack from going all the way into the clearing agent. We get
ours from W.A.
We are having a very similar problem too. We change our alcohols and Histoclear
like mad, but are having eosin bleeding out of the sections. I'm starting to
wonder if it is the mounting media on our glass coverslipper. I ordered new
bottle gaskets, so I guess I'll see what happens then.
Angela
All,
Is anyone performing paraffin block IHC staining for IgG4? If so, a vendor
name would be much appreciated.
Thank-you,
Glen Dawson
Milwaukee, WI
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Glen,
We had the same situation about a year ago. We sent the block and a bucal
smear out for DNA analysis.
Now we have the Vantage system to assure positive patient id. Check into it.
It saves time and worry and assures patient safety.
Jan
-Original Message-
From:
Our Molecular Pathology Laboratory offers a multiplex PCR assay for identity
markers for these situations. They receive specimens from all over the country
for this testing (usually specimen mix-ups). It's not cheap and they only bill
the referring facility.
Richard
Richard W. Cartun, Ph.D.
You would send out for DNA fingerprinting. We have used the Cleveland Clinic
for this.
Christie
From: janice.maho...@alegent.org
To: gdaw...@dynacaremilwaukee.com; histonet@lists.utsouthwestern.edu
Date: Mon, 24 Aug 2009 13:41:40 -0500
Subject: RE: [Histonet] Block Verification
CC:
I'm getting the same eosin bleeding and water on the slides. I'm using Hemo-D
and CitriSolv. I have changed the reagents and am still getting the eosin
bleeding, the water on the slide is a new phenomenon.
Any suggestions?
Paula Sicurello
VA Medical Center San Diego
Veterans Medical
If your solutions are clean the cause may be that the level of the water wash
station on your stainer is higher than the level of the subsequent alcohol
container which means that some water will not be removed from the top of the
slide. This is common with running water washes on stainers as
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