Hi all, Since the key to the question lies in Optimal Fixation, the answer to
this question is A, and it is taken right out of the Histotechnology
self-instructional text 2nd edition pg 98. (See note)
Under Frozen Sections
I've included a procedure I have used successfully in the past. It diff
8-3991│email: mckenzie.em...@mhsil.com
>
> -Original Message-
> From: histonet-boun...@lists.utsouthwestern.edu
> [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lee & Peggy
> Wenk
> Sent: Thursday, October 03, 2013 9:22 AM
> To: Watson, Linda; Stephenson
Please see Freida Carson's Histotechnology-A self Instructional text (2nd
edition pg 98, or 3rd edition pps 117-118). There you will find that the
correct answer is "A"- 37-40 % formaldehyde. In the Notes section she explains
"Many laboratories use acetone or alcohol as the fixative for frozen
rn.edu"
Date: 10/03/2013 11:42 AM
Subject:RE: [Histonet] HT HistoDeck question...
Sent by:histonet-boun...@lists.utsouthwestern.edu
For those of you who use the 40% formaldehyde, how long is you fixation
time on frozen slides? We use 10% NBF with 1 minute to fix, but some
-boun...@lists.utsouthwestern.edu] Im Auftrag von Rathborne,
Toni
Gesendet: Donnerstag, 03. Oktober 2013 20:34
An: 'Jennifer MacDonald'; Grantham, Andrea L - (algranth)
Cc: HISTONET; histonet-boun...@lists.utsouthwestern.edu
Betreff: RE: [Histonet] HT HistoDeck question...
For those of you
al Message- From: Stephenson, Sheryl
> Sent: Thursday, October 03, 2013 7:21 AM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] HT HistoDeck question...
>
> Hi,
> Please clarify why this answer to the HistoDeck study question is a) and not
> b).
>
&
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jennifer
MacDonald
Sent: Thursday, October 03, 2013 2:04 PM
To: Grantham, Andrea L - (algranth)
Cc: HISTONET; histonet-boun...@lists.utsouthwestern.edu
Subject: Re: [Histonet] HT HistoDeck question...
We also use this oil red O method and use the 40
(algranth)"
To:
Cc: HISTONET
Date: 10/03/2013 08:33 AM
Subject: Re: [Histonet] HT HistoDeck question...
Sent by:histonet-boun...@lists.utsouthwestern.edu
I'd go with "A", but it really depends on what you are going to do with
the sections after fixation
I'd go with "A", but it really depends on what you are going to do with the
sections after fixation.
In the protocol for Oil Red O in Freida's second edition (that I use almost
daily combined with some steps from PolyScientific's ORO protocol), step #1
says to fix in 40% formaldehyde. Doesn't s
Wenk
Sent: Thursday, October 03, 2013 9:22 AM
To: Watson, Linda; Stephenson, Sheryl; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] HT HistoDeck question...
I agree, there is probably more than one correct answer to this question,
depending upon whether you are planning on doing stains
er 03, 2013 9:40 AM
To: Lee & Peggy Wenk ; Stephenson, Sheryl ;
histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] HT HistoDeck question...
For frozen cut sections, would the fixation also depend on what you plan on
doing with it. For example, H&E, Special Stain or IHC? Please correct
: RE: [Histonet] HT HistoDeck question...
We fix H&E's in 95% and our IHC protocol is acetone/alcohol fixation.
Bernice Frederick HTL (ASCP)
Senior Research Tech
Pathology Core Facility
ECOGPCO-RL
Robert. H. Lurie Cancer Center
Northwestern University
710 N Fairbanks Court
Olson 8-4
u
Subject: RE: [Histonet] HT HistoDeck question...
For frozen cut sections, would the fixation also depend on what you plan on
doing with it. For example, H&E, Special Stain or IHC? Please correct if I am
wrong. I think that is a trick question!!!
>-Original Message-
o:histonet-
>boun...@lists.utsouthwestern.edu] On Behalf Of Lee & Peggy Wenk
>Sent: Thursday, October 03, 2013 8:16 AM
>To: Stephenson, Sheryl; histonet@lists.utsouthwestern.edu
>Subject: Re: [Histonet] HT HistoDeck question...
>
>Personally, I think it's "a" is a
iginal Message-
From: Stephenson, Sheryl
Sent: Thursday, October 03, 2013 7:21 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] HT HistoDeck question...
Hi,
Please clarify why this answer to the HistoDeck study question is a) and
not b).
Here is the question:
'Frozen section
Hi,
Please clarify why this answer to the HistoDeck study question is a) and not
b).
Here is the question:
'Frozen section slides cut from fresh, unfixed tissue specimens are optimally
fixed in which of the following solutions?
a) 37%-40% formaldehyde
b) Cold acetone
c) Aceti
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