[ccp4bb] Postdoctoral Research Fellow position Monash University, Australia

[Jennifer Huynh]

Postdoctoral Research Fellow position Monash University, Australia

Department of Biochemistry and Molecular Biology, Infection & Immunity
Program
Biomedicine Discovery Institute
Faculty of Medicine, Nursing and Health Sciences

Prof. Jamie Rossjohn FAA FAHMS FLSW FMedSci, ARC Australian Laureate Fellow
& Head, Infection and Immunity Program, Monash Biomedicine Discovery
Institute, seeks a postdoctoral researcher to join his research group to
work in the area of structural immunology, at Monash University, Clayton
campus, Melbourne, Australia.

The Rossjohn laboratory has provided profound insight into the molecular
bases underpinning lipid- mediated immunity (e.g. Nature 2007, Immunity
2009, 2011, Nature Immunol. 2012, 2015 & 2016), and seeks to build upon
these initial findings in the context of protective, tumour and aberrant
immunity. The laboratory is funded by grants from the NHMRC and ARC.

The role: seeking highly motivated and talented post- doctoral scientist to
join the research group. The selected candidates will work in the area of lipid
mediated immunity and structural biology. For more information:
http://research.med.monash.edu.au/rossjohn/

Postdoctoral research position: the applicant should hold a PhD in
Biochemistry, or Structural Biology with a background in Protein Chemistry
and Crystallography, with some experience in molecular biology, cell
culture (E. coli, insect cells and/or mammalian expression systems),
protein biochemistry, and/or immunology.

Candidates with a promising track record in the relevant areas and a proven
publication record in international journals are encouraged to apply.

Appointment will be made at a level appropriate to the successful
applicant’s qualifications, experience and in accordance with
classification standards for each level.

Salary range: Duration:

Level A $81,486 - $87,471 or Level B $92,074 - $109,339,

considered, to Jennifer Huynh (jennifer.hu...@monash.edu).

Closing Date: Sept 2, 2018

Further info can be found here: http://careers.pageuppeople.
com/513/cw/en/job/580071/research-fellow

-- 
*JENNIFER HUYNH*
*Senior Administration Coordinator *
*Office of Prof. Jamie Rossjohn, FAA FAHMS FLSW FMedSci*
*ARC Australian Laureate Fellow*

*Department of Biochemistry & Molecular Biology/ *
*School of Biomedical Sciences*
Monash University
Ground floor, Building 76, Clayton Campus
19 Innovation Walk
Clayton VIC 3800
Australia

T: +61 3 990 29252
F: +61 3 990 55645
E: jennifer.hu...@monash.edu 
(Please note my email is not jenny.hu...@monash.edu)
W: http://research.med.monash.edu.au/rossjohn/

Twitter: @RossjohnLab 
Facebook: *theRossjohnLab *
*Please note: I work part-time Tuesdays, Wednesdays & Thursdays in the
office*



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[ccp4bb] Postdoctoral position in RNA Epigenetics at UT Health San Antonio, Texas

[Yogesh Gupta]

*Postdoctoral Fellow position in RNA Epigenetics at UT Health San Antonio,
San Antonio, Texas*



The laboratory of Dr. Yogesh Gupta (http://ccri.uthscsa.edu/YGupta.html) at
The University of Texas Health Science Center in San Antonio (UT Health San
Antonio™) is seeking a highly motivated and enthusiastic scientist with a
strong interest in mechanistic studies on large protein-nucleic acid
assemblies that play critical roles in leukemia and pediatric sarcoma
pathogenesis. We are particularly interested in understanding the exact
mechanisms by which different enzymes and accessory factors cross talk,
assemble, and dynamically regulate the covalent chemical modifications on
both coding and non-coding RNA transcripts. We employ a powerful
combination of structural and chemical biology tools with an array of other
biophysical, *in silico*, and cell-based methods to address prevailing
questions for the emerging field of RNA epigenetics.



Our lab is physically located within the Greehey Children’s Cancer Research
Institute  (Greehey CCRI) at UT Health San
Antonio.  We have full access to an extensive array of core institutional
facilities (Biochemistry core
, CIDD ,
Research core labs ) located
within the Department of Biochemistry and Structural Biology, the Joe R.
and Teresa Lozano Long School of Medicine, and Greehey CCRI.  Applicants
with demonstrated experience in protein biochemistry, sarcoma or leukaemia
biology with experience in cell imaging, tissue culture, FACS, mice,
Cas/CRISPR knock out, and a keen interest in mechanistic/structural studies
are preferred. Candidates are expected to possess strong organizational,
interpersonal skills, and ability to work as part of a team. Candidate
should hold (or soon expected to hold) a PhD or equivalent degree in a
related discipline. Prior experience in X-ray crystallography or NMR is not
required.



Please visit the websites below to learn more about our work and future
directions –

http://ccri.uthscsa.edu/YGupta.html



If interested in applying, please send a short description of your research
accomplishments, and current CV with a list of three (3) references to Dr.
Yogesh Gupta (email: gup...@uthscsa.edu).



The Greehey Children’s Cancer Research Institute is a unique specialized
cancer research center focusing on basic and translational research in
childhood cancer, and occupies a state-of-the-art 100,000 sq. foot research
facility on the university’s Greehey Academic and Research Campus.



San Antonio is the nation’s seventh largest city and is located at the edge
of the beautiful Texas Hill County. San Antonio offers a rich,
multi-cultural community, affordable cost of living, excellent weather and
a thriving biomedical industry.



*All Postdoctoral appointments are designated as security sensitive
positions. The University of Texas Health Science Center at San Antonio is
an Equal Opportunity/Affirmative Action Employer including protected
veterans and persons with disabilities.*

-- 
Yogesh Gupta, PhD
Assistant Professor of Biochemistry & Structural Biology
PI, Greehey Children's Cancer Research Institute
University of Texas Health Science Center
8403 Floyd Curl Drive, San Antonio, Texas, USA 78229
http://ccri.uthscsa.edu/YGupta.html



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[ccp4bb] ccp4 release 7.0 update 061

[Charles Ballard - UKRI STFC]

Dear All

ccp4 update 061 to release 7.0 is now available.  It contains

* DIALS 1.10.4 and XIA2 0.5.582
 - release notes: https://github.com/dials/dials/releases/tag/v1.10.0

* molprobity 4.4
 - scripts molprobity.molprobity, molprobity.clashscore, molprobity.cablam, 
probe, reduce, etc
 - http://molprobity.biochem.duke.edu/
 - thank you to Jane Richardson, Nigel Moriarty, Randy Read

* lorestr
 - update to use bundled molprobity.

This is available as an update to current installations, and as a direct 
download from the ccp4 site.

Coming soon:
 
 * major update to monomer library
 * molprobity integration to ccp4i2
 * DIALS gui update
 * additional reference structures for buccaneer.

All the best

Charles 


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Re: [ccp4bb] AW: [ccp4bb] crystals that dont diffract :( :(

[jai mohan]

 Dear Careina,
Please make sure that your crystals are protein or salt, possibly using Izit 
dye.Note: there is no correlation so far hypothesized on the diffraction of 
ugly / beautiful / needle / plate type crystalsI believe!

With best regardsS.M.Jaimohan PhD
On Tuesday, 14 August, 2018, 7:21:09 PM IST, ferrer 
 wrote:  
 
  
 
 On 14/08/2018 12:27, Careina Edgooms wrote:
  
   Cryoprotectant was 50% PEG just added to the buffer that it crystallised in. 
It crystallised under batch.   
 which kind of batch plate ? Some are not suitable to in situ.
 
 JL
 
   
  On Tuesday, August 14, 2018, 12:11:17 PM GMT+2, Hughes, Jon 
 wrote:  
  
  
maybe it's the cryobuffer that's the problem (you didn't mention it). you could 
try to fish the crystals with minimal liquid attached by mounting them in oil 
rather than a cryobuffer. or you could test the native diffraction "in  situ" 
(at room temperature in the drop): quite a few beamlines offer this possibility 
these days. 
 
best
 
jon
 
  

Von: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] Im Auftrag von Careina 
Edgooms
 Gesendet: Dienstag, 14. August 2018 11:59
 An: CCP4BB@JISCMAIL.AC.UK
 Betreff: [ccp4bb] crystals that dont diffract :( :(
   
  
   
I got the most beautiful crystals I have ever seen and they don't diffract at 
all. Not poor diffraction, NO  diffraction. Anyone know why this could be and 
how I can go about fixing it? I had three beautiful crystals and not one 
diffracted. I did leave them in the drop for about 3 weeks before harvesting 
and in  liquid nitrogen for about a month before diffracting. Could that be a 
factor? If I regrew more beautiful crystals and diffracted straight away could 
that help?
   
Careina
   
  

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 -- 

Jean-Luc Ferrer 
Institut de Biologie Structurale
71 Avenue des Martyrs   
CS 10090
38044 Grenoble Cedex 9 - FRANCE

Ph.:  +33 (0)4 57 42 85 22
Cell: +33 (0)6 89 45 13 57
email: jean-luc.fer...@ibs.fr

 

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[ccp4bb] Full Professorship/Chair of "Structural Biology" at the Technische Universität Berlin

[Holger Dobbek]

The Technische Universität Berlin, Fac­ulty of Pro­cess Sci­ences, Insti­tute 
of Bio­tech­no­logy has an opening for a chair/University Professor (W3) of 
"Structural Biology“. 

For details about the research profile, requirements and how to apply, please 
follow the link:
https://stellenticket.de/50129/TUB/?lang=en 


Applications should be sent until 24th of August.

On behalf of the search committee,

Holger Dobbek
Strukturbiologie/Biochemie
Institut für Biologie
Humboldt-Universität zu Berlin
Germany
Tel.:+49 30 2093 6369
holger.dob...@biologie.hu-berlin.de 


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[ccp4bb] AW: Re: [ccp4bb] crystals that dont diffract :( :(

[Hughes, Jon]

Yes indeed!
Jon

--
Jon Hughes
(+49/0)1757929098
Sent without the use of Apple products.

 Daniel M. Himmel, Ph. D. schrieb 

Dear JL,

Years ago, this was a common problem when I was crystallizing myosin
constructs for my doctoral work.  Some of the most beautiful crystals I
got showed little or no diffraction.  Often this occurs when there is a very
large water content in the asymmetric unit and in proteins that have a
great deal of intrinsic disorder.  Jon's suggestion to flash-cool them in
oil could work.  Your high PEG concentration in the flash-cooling solution
might work, too, but you probably cannot just plunge your crystals
suddenly into a much higher PEG solution.  A few suggestions:

1) Whichever cryoprotectant you use, introduce it to your crystal GRADUALLY,
such as in steps (e.g., 10% ==> 15% ==> 20% ==>25%) or something like that.
For some proteins, drastic rapid changes of concentrations of anything in
the solution can damage the crystal and introduce enough disorder so that
the protein crystal will not diffract well.  Sometimes you can tell when there's
damage if the crystal cracks (or melts away), but not always.

2) You may have to experiment with different cryoprotectants.  Different
cryoprotectants make different protein crystals happy.  For example, try PEG 
200,
PEG 400, PEG 600, glycerol, sucrose, trehalose, other disaccharide sugars, MPD, 
butanediols.

3) I have found that, as a "rule of thumb", 25% of any cryoprotectant is enough
to protect against ice formation.  If your protein crystal can tolerate it, 
higher
concentrations could be better (because they can shrink the unit cell and reduce
some of the water content).  HOWEVER, many protein crystals will not tolerate
much higher concentrations without taking on damage.

I hope this helps.

-Daniel

On Tue, Aug 14, 2018 at 9:45 AM, ferrer 
mailto:jean-luc.fer...@ibs.fr>> wrote:
Hi

Did you try them at room temp, in situ (straight in the plate). We observe that 
time to time on our beamline, when just harvesting, not mentioning cryo 
protection, is enough to loose all diffraction. It-s rare but happens.

Regards

JL

On 14/08/2018 11:58, Careina Edgooms wrote:
I got the most beautiful crystals I have ever seen and they don't diffract at 
all. Not poor diffraction, NO diffraction. Anyone know why this could be and 
how I can go about fixing it? I had three beautiful crystals and not one 
diffracted. I did leave them in the drop for about 3 weeks before harvesting 
and in liquid nitrogen for about a month before diffracting. Could that be a 
factor? If I regrew more beautiful crystals and diffracted straight away could 
that help?
Careina



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--

Jean-Luc Ferrer
Institut de Biologie Structurale
71 Avenue des 
Martyrs
CS 10090
38044 Grenoble Cedex 9 - FRANCE

Ph.:  +33 (0)4 57 42 85 22
Cell: +33 (0)6 89 45 13 57
email: jean-luc.fer...@ibs.fr





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Re: [ccp4bb] Fishing crystals from volatile solvent as precipitant

[Yvonne Thielmann]

Dear Thomas,

maybe you can try to overlay your drop with LV CryoOil from Jena 
Bioscience. Then the evaporation of the solvent is slowed down and the 
crystals are directly cryoprotected when you move the crystals through 
the oil. We had quite good results when we cryoprotected crystals in 
this oil.


Best wishes,
Yvonne


--
Dr. Yvonne Thielmann
Max Planck Institute of Biophysics
Molecular Membrane Biology
Max-von-Laue-Strasse 3
60438 Frankfurt / Main
Germany

Office +49 69 6303 1056
Lab +49 69 6303 1074
Fax +49 69 6303 1002

Am 15.08.2018 um 08:05 schrieb Kajander, Tommi A:

Yes sorry, i meant paratone-N also.

Tommi

Kohteesta: ferrer
Lähetetty: keskiviikko 15. elokuuta klo 0.41
Aihe: Re: [ccp4bb] Fishing crystals from volatile solvent as precipitant
Vastaanottaja: ccp4bb@jiscmail.ac.uk


Dear Thomas,

Alternatively you can try shooting on crystals in the drop, in situ. So 
fishing, no cryo. But potentially high radiation damage. Can be 
considered if you have enough crystals, and if your crystallization 
plate makes it possible.


Regards

JL

On 14/08/2018 20:58, Thomas Krey wrote:
Dear crystallization experts,

We have 3D protein crystals grown from a microseed matrix screening 
vapor diffusion experiment in either


15% (v/v) Reagent alcohol
HEPES Na pH 7.5
0.2 M MgCl2

or in

27% Isopropanol
0.18 M MgCl2
90 mM HEPES Na pH 7.5
10% Glycerol

Upon opening the corresponding wells these crystals move quite a bit – 
presumably due to the volatility of the alcohols. Does anyone have a 
good suggestion to stabilize the swirling movements? Does anyone have 
experience, whether these conditions alone can serve as cryo-protectant 
(i.e., do we really have to fish, move into cryo solution and fish again)?

Any suggestion or input would be highly welcome.

Thank you very much in advance.

Thomas


Prof. Dr. Thomas Krey
Hannover Medical School
Institute of Virology
Structural Virology Group
Carl-Neuberg-Str. 1
D-30625 Hannover
phone: +49 (0) 511 - 532 4308
email: krey.tho...@mh-hannover.de 


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--  Jean-Luc Ferrer Institut de Biologie 
Structurale 71 Avenue des Martyrs CS 10090 38044 Grenoble Cedex 9 - 
FRANCE Ph.: +33 (0)4 57 42 85 22 Cell: +33 (0)6 89 45 13 57 email: 
jean-luc.fer...@ibs.fr  


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Re: [ccp4bb] Fishing crystals from volatile solvent as precipitant

[Kajander, Tommi A]

Yes sorry, i meant paratone-N also.

Tommi

Kohteesta: ferrer
Lähetetty: keskiviikko 15. elokuuta klo 0.41
Aihe: Re: [ccp4bb] Fishing crystals from volatile solvent as precipitant
Vastaanottaja: ccp4bb@jiscmail.ac.uk


Dear Thomas,

Alternatively you can try shooting on crystals in the drop, in situ. So 
fishing, no cryo. But potentially high radiation damage. Can be considered if 
you have enough crystals, and if your crystallization plate makes it possible.

Regards

JL

On 14/08/2018 20:58, Thomas Krey wrote:
Dear crystallization experts,

We have 3D protein crystals grown from a microseed matrix screening vapor 
diffusion experiment in either

15% (v/v) Reagent alcohol
HEPES Na pH 7.5
0.2 M MgCl2

or in

27% Isopropanol
0.18 M MgCl2
90 mM HEPES Na pH 7.5
10% Glycerol

Upon opening the corresponding wells these crystals move quite a bit – 
presumably due to the volatility of the alcohols. Does anyone have a good 
suggestion to stabilize the swirling movements? Does anyone have experience, 
whether these conditions alone can serve as cryo-protectant (i.e., do we really 
have to fish, move into cryo solution and fish again)?
Any suggestion or input would be highly welcome.

Thank you very much in advance.

Thomas


Prof. Dr. Thomas Krey
Hannover Medical School
Institute of Virology
Structural Virology Group
Carl-Neuberg-Str. 1
D-30625 Hannover
phone: +49 (0) 511 - 532 4308
email: krey.tho...@mh-hannover.de


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--  Jean-Luc Ferrer Institut de Biologie 
Structurale 71 Avenue des Martyrs CS 10090 38044 Grenoble Cedex 9 - FRANCE Ph.: 
+33 (0)4 57 42 85 22 Cell: +33 (0)6 89 45 13 57 email: 
jean-luc.fer...@ibs.fr 

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