Dear Thomas,
maybe you can try to overlay your drop with LV CryoOil from Jena
Bioscience. Then the evaporation of the solvent is slowed down and the
crystals are directly cryoprotected when you move the crystals through
the oil. We had quite good results when we cryoprotected crystals in
this oil.
Best wishes,
Yvonne
--
Dr. Yvonne Thielmann
Max Planck Institute of Biophysics
Molecular Membrane Biology
Max-von-Laue-Strasse 3
60438 Frankfurt / Main
Germany
Office +49 69 6303 1056
Lab +49 69 6303 1074
Fax +49 69 6303 1002
Am 15.08.2018 um 08:05 schrieb Kajander, Tommi A:
Yes sorry, i meant paratone-N also.
Tommi
Kohteesta: ferrer
Lähetetty: keskiviikko 15. elokuuta klo 0.41
Aihe: Re: [ccp4bb] Fishing crystals from volatile solvent as precipitant
Vastaanottaja: ccp4bb@jiscmail.ac.uk
Dear Thomas,
Alternatively you can try shooting on crystals in the drop, in situ. So
fishing, no cryo. But potentially high radiation damage. Can be
considered if you have enough crystals, and if your crystallization
plate makes it possible.
Regards
JL
On 14/08/2018 20:58, Thomas Krey wrote:
Dear crystallization experts,
We have 3D protein crystals grown from a microseed matrix screening
vapor diffusion experiment in either
15% (v/v) Reagent alcohol
HEPES Na pH 7.5
0.2 M MgCl2
or in
27% Isopropanol
0.18 M MgCl2
90 mM HEPES Na pH 7.5
10% Glycerol
Upon opening the corresponding wells these crystals move quite a bit –
presumably due to the volatility of the alcohols. Does anyone have a
good suggestion to stabilize the swirling movements? Does anyone have
experience, whether these conditions alone can serve as cryo-protectant
(i.e., do we really have to fish, move into cryo solution and fish again)?
Any suggestion or input would be highly welcome.
Thank you very much in advance.
Thomas
Prof. Dr. Thomas Krey
Hannover Medical School
Institute of Virology
Structural Virology Group
Carl-Neuberg-Str. 1
D-30625 Hannover
phone: +49 (0) 511 - 532 4308
email: krey.tho...@mh-hannover.de <mailto:krey.tho...@mh-hannover.de>
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