Yes sorry, i meant paratone-N also. Tommi
Kohteesta: ferrer Lähetetty: keskiviikko 15. elokuuta klo 0.41 Aihe: Re: [ccp4bb] Fishing crystals from volatile solvent as precipitant Vastaanottaja: ccp4bb@jiscmail.ac.uk Dear Thomas, Alternatively you can try shooting on crystals in the drop, in situ. So fishing, no cryo. But potentially high radiation damage. Can be considered if you have enough crystals, and if your crystallization plate makes it possible. Regards JL On 14/08/2018 20:58, Thomas Krey wrote: Dear crystallization experts, We have 3D protein crystals grown from a microseed matrix screening vapor diffusion experiment in either 15% (v/v) Reagent alcohol HEPES Na pH 7.5 0.2 M MgCl2 or in 27% Isopropanol 0.18 M MgCl2 90 mM HEPES Na pH 7.5 10% Glycerol Upon opening the corresponding wells these crystals move quite a bit – presumably due to the volatility of the alcohols. Does anyone have a good suggestion to stabilize the swirling movements? Does anyone have experience, whether these conditions alone can serve as cryo-protectant (i.e., do we really have to fish, move into cryo solution and fish again)? Any suggestion or input would be highly welcome. Thank you very much in advance. Thomas Prof. Dr. Thomas Krey Hannover Medical School Institute of Virology Structural Virology Group Carl-Neuberg-Str. 1 D-30625 Hannover phone: +49 (0) 511 - 532 4308 email: krey.tho...@mh-hannover.de<mailto:krey.tho...@mh-hannover.de> To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 -- -------------------------------- Jean-Luc Ferrer Institut de Biologie Structurale 71 Avenue des Martyrs CS 10090 38044 Grenoble Cedex 9 - FRANCE Ph.: +33 (0)4 57 42 85 22 Cell: +33 (0)6 89 45 13 57 email: jean-luc.fer...@ibs.fr<mailto:jean-luc.fer...@ibs.fr> -------------------------------- To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
