Dear Careina,
Please make sure that your crystals are protein or salt, possibly using Izit
dye.Note: there is no correlation so far hypothesized on the diffraction of
ugly / beautiful / needle / plate type crystals....I believe!
With best regardsS.M.Jaimohan PhD
On Tuesday, 14 August, 2018, 7:21:09 PM IST, ferrer
<jean-luc.fer...@ibs.fr> wrote:
On 14/08/2018 12:27, Careina Edgooms wrote:
Cryoprotectant was 50% PEG just added to the buffer that it crystallised in.
It crystallised under batch.
which kind of batch plate ? Some are not suitable to in situ.
JL
On Tuesday, August 14, 2018, 12:11:17 PM GMT+2, Hughes, Jon
<jon.hug...@bot3.bio.uni-giessen.de> wrote:
maybe it's the cryobuffer that's the problem (you didn't mention it). you could
try to fish the crystals with minimal liquid attached by mounting them in oil
rather than a cryobuffer. or you could test the native diffraction "in situ"
(at room temperature in the drop): quite a few beamlines offer this possibility
these days.
best
jon
Von: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] Im Auftrag von Careina
Edgooms
Gesendet: Dienstag, 14. August 2018 11:59
An: CCP4BB@JISCMAIL.AC.UK
Betreff: [ccp4bb] crystals that dont diffract :( :(
I got the most beautiful crystals I have ever seen and they don't diffract at
all. Not poor diffraction, NO diffraction. Anyone know why this could be and
how I can go about fixing it? I had three beautiful crystals and not one
diffracted. I did leave them in the drop for about 3 weeks before harvesting
and in liquid nitrogen for about a month before diffracting. Could that be a
factor? If I regrew more beautiful crystals and diffracted straight away could
that help?
Careina
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Jean-Luc Ferrer
Institut de Biologie Structurale
71 Avenue des Martyrs
CS 10090
38044 Grenoble Cedex 9 - FRANCE
Ph.: +33 (0)4 57 42 85 22
Cell: +33 (0)6 89 45 13 57
email: jean-luc.fer...@ibs.fr
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