[Freesurfer] mri_thickness mri_make_surfaces

2014-10-09 Thread Seung-Goo KIM
Hi all,

I’m trying to compute cortical thickness using mris_thickness (stable 5), but 
getting slightly different results from the recon-all 5.1.0-amd64-2.13 
(mri_make_surfaces, stable 5).

Hinted from an old thread: 
https://www.mail-archive.com/freesurfer@nmr.mgh.harvard.edu/msg05994.html, I 
set the switch “-N 20, but it returns  different values: 50% of vertices have 
identical values with the cross-correlation of about 0.98 and RMS of 0.08 mm, 
which is of course very high, but a bit odd while I was expecting nearly 
identical results. When I checked the scatter plots, the mris_thickness returns 
a bit smaller values for many vertices than the standard result. Is this 
somewhat expected difference between mri_make_surfaces and mris_thickness?

Also I found that mri_thickness reads “?h.gray”, not “?h.pial”. As I assumed 
that it reads “?h.pial” and “?h.white” to compute thickness, so I just linked 
“?h.pial” to “?h.gray”.  Is this assumed input for mris_thickness?


Best regards,
-- 
Seung-Goo KIM

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Re: [Freesurfer] WM Pial Segmentation Errors

2014-10-09 Thread Varjabedian, Ani
Hi Michael, 

I took a look at your data and it seems that you paired a T2 image with
a T1 image when you submitted to recon all. Recon-all will try to
average these together, and since they have such different contrasts,
you will end up with a poor template for surfaces later on. 

Try running recon-all again without the T2 and see if that helps.


-Ani 


On Wed, 2014-10-08 at 17:21 +, Smith, Michael wrote:
 Ah, sorry for some reason it just wasn’t clicking before.  I was able to
 upload a dataset.  Thanks!
 
 On 10/8/14, 10:59 AM, Bruce Fischl fis...@nmr.mgh.harvard.edu wrote:
 
 Hi Michael
 
 you need to follow the directions on this page:
 
 http://surfer.nmr.mgh.harvard.edu/fswiki/FtpFileExchange
 
 in particular, make sure you change directories before trying to put
 
 cheers
 Bruce
 
 On Wed, 
 8 Oct 2014, 
 Smith, 
 Michael wrote:
 
  See attached
 
  On 10/8/14, 10:46 AM, Bruce Fischl fis...@nmr.mgh.harvard.edu wrote:
 
  can you send us the details of your ftp command?
  On Wed, 8 Oct 2014, Smith,
  Michael wrote:
 
  Hi Bruce,
 
  Do I not have permission to transfer files to the remote machine? I
 can
  login fine as an anonymous user but when I attempt to use the put
  command
  it says it could not create the file.
 
  On 10/7/14, 7:33 PM, Bruce Fischl fis...@nmr.mgh.harvard.edu
 wrote:
 
  Hi Michael
 
  sure, the T2 can help if you have it (although you'll need to
 download
  some
  updated binaries), but it is impossible to tell from these images
 what
  is
  going on. Looks either the intensity normalization, mri_segment or
  mris_make_surfaces failed. You can try the expert options for each
 one
  to
  prespecify the range of allowable intensities for gray and white
  matter.
  If
  you upload the subject to our ftp site (the whole subject directory
  tarred
  and gzipped) we will take a look
 
  cheers
  Bruce
 
  On Mon, 6 Oct 2014, Smith, Michael wrote:
 
  Hi,
 
  I¹ve been having trouble on a few of our subjects with getting the
  white
  matter and pial surface files to ³line up² correctly with the volume
  files.
   I¹ve attached a screenshot of what is happening: the pial surface
 is
  in red
  and the white matter surface file is in yellow. I have also attached
  an
  example of a ³good² subject for comparison.
 
  I¹ve tried looking through the tutorials online but didn¹t see
  anything
  that
  was really related to my issue and I was wondering what the best way
  to
  correct this.  We have T2 weighted images for several subjects is
 well
  if
  that helps.  Not sure if Freesurfer can use T2 images instead of T1
 in
  the
  ­reconall script but I thought I would mention it.  Thank you very
  much
  in
  advance!
 
  Best,
 
  Michael S.
 
 
 
 
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[Freesurfer] deployed (compiled) MATLAB scripts path manipulation

2014-10-09 Thread slehar
I have been following the directions on this site to deploy (compile) my
MATLAB script so as to be able to run it on launchpad without worrying
about MATLAB licenses.

https://nmr.mgh.harvard.edu/martinos/itgroup/deploytool.html

They say that the deployed script cannot do path manipulations such as
addpath() or path(path,xxx), so the script should use isdeployed to make
sure that such path commands are only executed if isdeployed == 0.


% Only add path if not deployed
if isdeployed==0;
  addpath ~nummenma/matlab/work/connectome/HCP_preproc_tools;
end

but then I need to add the path back in again so it can access the
functions on that path. In the deploytool GUI I click Settings (the little
gear icon), and in the upper window Additional Parameters passed to MCC:
I inserted

==[ Additional Parameters passed to MCC: ]==
-I ~nummenma/matlab/work/connectome/HCP_preproc_tools
mris_gradient_nonlin__unwarp_volume__batchmode_HCPS.m


(A web site I found said you need a path to the m-file itself, not just to
the directory that contains it, and you separate the path and file)

Then I found I had to circumvent more path(path,xxx) commands in my
startup.m file too

==[ startup.m ]==
% If script is not deployed add paths
if isdeployed==0;
  % FreeSurfer -%
  fshome = getenv('FREESURFER_HOME');
  fsmatlab = sprintf('%s/matlab',fshome);
  if (exist(fsmatlab) == 7)
  path(path,fsmatlab);
  end
  clear fshome fsmatlab;
  %-%

  % FreeSurfer FAST %
  fsfasthome = getenv('FSFAST_HOME');
  fsfasttoolbox = sprintf('%s/toolbox',fsfasthome);
  if (exist(fsfasttoolbox) == 7)
  path(path,fsfasttoolbox);
  end
  clear fsfasthome fsfasttoolbox;
  %-%
end
=

Now at least it compiles without error. But when I run it I get this:

Set up FreeSurfer environment
 freesurfer-Linux-centos6_x86_64-stable-v5.3.0-20130514 
Setting up environment for FreeSurfer/FS-FAST (and FSL)
FREESURFER_HOME   /usr/local/freesurfer/stable5_3_0
FSFAST_HOME   /usr/local/freesurfer/stable5_3_0/fsfast
FSF_OUTPUT_FORMAT nii.gz
SUBJECTS_DIR  /space/ficus/4/users/SNEF_subjects
MNI_DIR   /usr/local/freesurfer/stable5_3_0/mni
FSL_DIR   /usr/pubsw/packages/fsl/current
./run_unwarpone.sh
/cluster/matlab/8.2
/space/ficus/4/users/SLOTS_sessions_Step/SLOTS010/aahscout/001/001.mgz
/space/ficus/4/users/SLOTS_sessions_unwarp/SLOTS010/aahscout/001/001.mgz
/space/ficus/4/users/SLOTS_sessions_Step/SLOTS010/unwarpscout001.log
--
Setting up environment variables
---
LD_LIBRARY_PATH is
.:/cluster/matlab/8.2/runtime/glnxa64:/cluster/matlab/8.2/bin/glnxa64:/cluster/matlab/8.2/sys/os/glnxa64
The file
   '/usr/local/freesurfer/stable5_3_0/matlab/load_nifti.m'
   is not in the application's expanded CTF archive at
'/homes/6/slehar/.mcrCache8.2/unwarp2'.
This is typically caused by calls to ADDPATH in your startup.m or
matlabrc.m files. Please see the compiler documentation and use the
ISDEPLOYED function to ensure ADDPATH commands are not executed by
deployed applications.
An error occurred while trying to determine whether load_nifti is a
function name.

MATLAB:err_while_looking_up_function
Error:An error occurred while trying to determine whether load_nifti is
a function name.
Error:Error in mclFreeStackTrace.

Any idea what the problem is now? load_nifti is presumably a FreeSurfer
function -- do I now have a problem with my paths to FreeSurfer? I
searched my script code and found no explicit call to load_nifti, I
presume that is called deep inside some other function. Should I add the
FreeSurfer paths also through the deploytool GUI and not call nmrenv?

(from my .cshrc file...
alias nmrenv 'source /usr/local/freesurfer/nmr-stable53-env'
)

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[Freesurfer] excluding labels

2014-10-09 Thread Adam, Ruth
Hi all,Would be great is someone can tell me how to exclude user-definedlabels from the analysis.As far as I understand after creating all labels, mris_label2annot with a custom colortable should be used.-Is this correct or is there a more efficient way to exclude labels form analysis?-How can the new annotation file be incorporated with old annotation files to mark what should be excluded/included in the analysis?-Can a similar operation be done directly for labels created in Tkmedit and volumes?Thank you very much in advance!Best,ruthi___
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[Freesurfer] 8 spots remain in the FreeSurfer course

2014-10-09 Thread Allison Stevens
Hello everyone,
We have 8 spots remaining in our upcoming FreeSurfer course happening on 
October 27-29. We will accept late registrations up to the course date; 
however, since the payment deadline has passed, payment is due immediately 
upon registration.

Details on the course and a link to the registration page can be found 
here:
https://surfer.nmr.mgh.harvard.edu/fswiki/CourseDescription

Note: This course is the only one where we are currently able to accept 
credit card. This likely won't be an option for future courses.
Allison

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[Freesurfer] FSGD Format Error

2014-10-09 Thread Retzepi, Kallirroi
Hi all,
I have been struggling with my .fsgd file. Its header is the following (a total 
of 119 subjects):

GroupDescriptorFile1
TitlePatients_vs_Controls
MeasurementNamethickness
ClassPxMale
ClassPxFemale
ClassConMale
ClassConFemale
ClassPxMPRAGE
ClassPxSPGR
ClassConMPRAGE
ClassConSPGR
ClassMaleMPRAGE
ClassMaleSPGR
ClassFemaleMPRAGE
ClassFemaleSPGR
VariablesAge
InputFS_1PxFemalePxMPRAGEFemaleMPRAGE51
..(rest of subjects)


I think something is up with my Class declaration, since the error message I 
get is the following:

ERROR: gdfReadV1: Input line 1, subjid = FS_1
   Found 3 variables, expected. 1
FSGDF Format Error: file = /group_stats/Px_vs_Con_A.fsgd, tag=Input



Any help would be greatly appreciated!!

Thank you!

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[Freesurfer] lgi mris_preproc

2014-10-09 Thread Lars M. Rimol
Hi,

In the wiki page on the gyrification index it says that to sample the
results to ico, use

recon-all -s my_subject_id -qcache -measure pial_lgi

If I prefer not to use qudec, can I instead use mris_preproc to do the
registration to atlas and concatenate the files and then use mri_surf2sruf
to smooth? And then proceed with inferential statistics?


Thank  you!

---
from the wiki:


The primary output file is ?h.pial_lgi, which can be viewed as an overlay
in tksurfer or freeview:

tksurfer my_subject_id lh inflated -overlay lh.pial_lgi

To perform a vertex-wise analysis in QDEC of the lgi surface data, first
sample the results to the average template subject 'fsaverage':

recon-all -s my_subject_id -qcache -measure pial_lgi

Do this for each of the subjects in your group




-- 
yours,

Lars M. Rimol, PhD
Norwegian University of Technology and Science (NTNU)
Trondheim,
Norway
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Re: [Freesurfer] lgi mris_preproc

2014-10-09 Thread Douglas Greve

you should be able to use mris_preproc

On 10/9/14 1:08 PM, Lars M. Rimol wrote:

Hi,

In the wiki page on the gyrification index it says that to sample the 
results to ico, use

recon-all -s my_subject_id -qcache -measure pial_lgi

If I prefer not to use qudec, can I instead use mris_preproc to do the 
registration to atlas and concatenate the files and then use 
mri_surf2sruf to smooth? And then proceed with inferential statistics?



Thank  you!

---
from the wiki:


The primary output file is ?h.pial_lgi, which can be viewed as an 
overlay in tksurfer or freeview:

tksurfer my_subject_id lh inflated -overlay lh.pial_lgi

To perform a vertex-wise analysis in QDEC of the lgi surface data, 
first sample the results to the average template subject 'fsaverage':


recon-all -s my_subject_id -qcache -measure pial_lgi
Do this for each of the subjects in your group




--
yours,

Lars M. Rimol, PhD
Norwegian University of Technology and Science (NTNU)
Trondheim,
Norway


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Re: [Freesurfer] FSGD Format Error

2014-10-09 Thread Douglas Greve


Each subject can only have one class. So, if you have males and females 
who are conotrols and ADs, then you would have 4 classes, and each 
subject could only belong to one class


On 10/9/14 11:55 AM, Retzepi, Kallirroi wrote:

Hi all,
I have been struggling with my .fsgd file. Its header is the following 
(a total of 119 subjects):


GroupDescriptorFile1
TitlePatients_vs_Controls
MeasurementNamethickness
Class PxMale
Class PxFemale
ClassConMale
ClassConFemale
ClassPxMPRAGE
ClassPxSPGR
ClassConMPRAGE
ClassConSPGR
ClassMaleMPRAGE
ClassMaleSPGR
ClassFemaleMPRAGE
ClassFemaleSPGR
VariablesAge
InputFS_1PxFemalePxMPRAGEFemaleMPRAGE51
..(rest of subjects)


I think something is up with my Class declaration, since the error 
message I get is the following:


ERROR: gdfReadV1: Input line 1, subjid = FS_1
   Found 3 variables, expected. 1
FSGDF Format Error: file = /group_stats/Px_vs_Con_A.fsgd, tag=Input



Any help would be greatly appreciated!!

Thank you!



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Re: [Freesurfer] excluding labels

2014-10-09 Thread Douglas Greve

What analysis?

On 10/9/14 11:35 AM, Adam, Ruth wrote:

Hi all,

Would be great is someone can tell me how to exclude 
user-defined labels from the analysis.


As far as I understand after creating all labels, mris_label2annot 
with a custom colortable should be used.
-Is this correct or is there a more efficient way to exclude labels 
form analysis?
-How can the new annotation file be incorporated with old annotation 
files to mark what should be excluded/included in the analysis?
-Can a similar operation be done directly for labels created in 
Tkmedit and volumes?


Thank you very much  in advance!

Best,
ruthi


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Re: [Freesurfer] Study design for qdec or command line group analysis

2014-10-09 Thread Sneha Pandya
Hi all,

Sorry for the follow up but hope someone can clarify following doubts.

Thanks,
Sneha

From: freesurfer-boun...@nmr.mgh.harvard.edu 
[freesurfer-boun...@nmr.mgh.harvard.edu] on behalf of Sneha Pandya 
[snp2...@med.cornell.edu]
Sent: Tuesday, October 07, 2014 1:06 PM
To: freesurfer@nmr.mgh.harvard.edu
Subject: [Freesurfer] Study design for qdec or command line group analysis

Dear freesurfers,

I have a study in which we want to perform group analysis to find the effect of 
age on cortical thickness between patients and controls. We have 11 patients 
with age group from 45-75 years and the mean of 53. We shall age match our 
controls with respect to similar age range and mean.

However our patient scans are from both 1.5T and 3.0T scanner, hence I have few 
questions about designing our study.


A) Can we treat certain discrete factors as continuous variables, like gender 
(male with value 1 and female with value 2), and scanner (1.5T with value 1 and 
3.0T with value 2) to make group analysis qdec-able in case of 3 discrete 
factors? I tried to look into mailing archive to figure out the answer, but so 
far found confounding answers.

B) If what asked above is possible can you please suggest which design I should 
opt for?
Design 1)
Three discrete factors, each with 2 levels:
(i) gender (male and female)
   (ii) groups (healthy and patients)
  (iii) scanner (1.5T and 3.0T),
Continuous variable:
(i) age

OR

Design 2)
Two  discrete factors, each with 2 levels
(i) gender (male and female)
   (ii) groups (healthy and patients)
Continuous variables:
(i) age
   (ii) scanner (1.5T as 1, and 3.0T as 0)

From literature we are not expecting any effect of gender and scanner on 
change of cortical thickness with age between groups, however I guess we need 
to control for it right?
Should I implement design 1 with mri_glmfit command line, as it has 3 discrete 
factors or design 2 in qdec?

B) We are expecting some change in cortical thickness with age between groups. 
Hence we shall be using DODS model to perform the analysis. In this case I 
should demean my ages right (subtract grand mean of age from combined healthy 
and patient groups and subtract from every individual age)?

Thanks,
Sneha

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Re: [Freesurfer] Surface Viewer Issue.

2014-10-09 Thread Jamie Hanson
 on
 functional data and extracting the thickness measures under that ROI. But
 this would be a little different

 Does this make sense, and is this doable in freesurfer? If so, could
 someone point me to the relevant tutorial or commands?

 I appreciate your help
 -- next part --
 An HTML attachment was scrubbed...
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 http://mail.nmr.mgh.harvard.edu/pipermail/freesurfer/attachments/20141008/9ecbc2cf/attachment-0001.html

 --

 Message: 7
 Date: Thu, 9 Oct 2014 00:12:04 -0400 (EDT)
 From: Reza Rajimehr r...@nmr.mgh.harvard.edu
 Subject: Re: [Freesurfer] rawfunc2surf-sess with a limited set of
 surface files
 To: freesurfer@nmr.mgh.harvard.edu
 Message-ID:
 42178.18.93.15.69.1412827924.squir...@mail.nmr.mgh.harvard.edu
 Content-Type: text/plain;charset=iso-8859-1

 Hi Doug,

 Removing -i fmc did not help, and I still get the same error.

 By looking at the rawfunc2surf-sess script, it looks like specifying an
 argument for -surfreg makes ExpKeyNeeded = 1. Can I just specify something
 for -expkey? Is this something that is used only in the name of log file?

 Thanks,
 Reza


 Try it without -i fmc


 On 10/08/2014 04:38 AM, Reza Rajimehr wrote:
  One more question:
 
  I tried to run:
 
  rawfunc2surf-sess -s oz_fparc -i fmc -trgsubject F99 -surfreg
  iter05.sphere.reg -fwhm 2 -per-session
 
  And I got this:
 
  ERROR: experts key needed (-expkey)
 
  Not sure what this flag is.
 
  Reza


 --

 Message: 8
 Date: Thu, 9 Oct 2014 12:42:07 +0200
 From: Seung-Goo KIM sol...@gmail.com
 Subject: [Freesurfer] mri_thickness  mri_make_surfaces
 To: freesurfer@nmr.mgh.harvard.edu
 Message-ID: 4e85c9bd-03cd-4781-a989-432d9eaf7...@gmail.com
 Content-Type: text/plain; charset=windows-1252

 Hi all,

 I?m trying to compute cortical thickness using mris_thickness (stable 5),
 but getting slightly different results from the recon-all 5.1.0-amd64-2.13
 (mri_make_surfaces, stable 5).

 Hinted from an old thread:
 https://www.mail-archive.com/freesurfer@nmr.mgh.harvard.edu/msg05994.html,
 I set the switch ?-N 20, but it returns  different values: 50% of vertices
 have identical values with the cross-correlation of about 0.98 and RMS of
 0.08 mm, which is of course very high, but a bit odd while I was expecting
 nearly identical results. When I checked the scatter plots, the
 mris_thickness returns a bit smaller values for many vertices than the
 standard result. Is this somewhat expected difference between
 mri_make_surfaces and mris_thickness?

 Also I found that mri_thickness reads ??h.gray?, not ??h.pial?. As I
 assumed that it reads ??h.pial? and ??h.white? to compute thickness, so I
 just linked ??h.pial? to ??h.gray?.  Is this assumed input for
 mris_thickness?


 Best regards,
 --
 Seung-Goo KIM

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 Message: 9
 Date: Thu, 9 Oct 2014 14:01:55 +
 From: Varjabedian, Ani avarjabed...@mgh.harvard.edu
 Subject: Re: [Freesurfer] WM  Pial Segmentation Errors
 To: Freesurfer support list freesurfer@nmr.mgh.harvard.edu
 Message-ID: 1412863314.22885.45.ca...@sangwoo.nmr.mgh.harvard.edu
 Content-Type: text/plain; charset=utf-8

 Hi Michael,

 I took a look at your data and it seems that you paired a T2 image with
 a T1 image when you submitted to recon all. Recon-all will try to
 average these together, and since they have such different contrasts,
 you will end up with a poor template for surfaces later on.

 Try running recon-all again without the T2 and see if that helps.


 -Ani


 On Wed, 2014-10-08 at 17:21 +, Smith, Michael wrote:
  Ah, sorry for some reason it just wasn?t clicking before.  I was able to
  upload a dataset.  Thanks!
 
  On 10/8/14, 10:59 AM, Bruce Fischl fis...@nmr.mgh.harvard.edu wrote:
 
  Hi Michael
  
  you need to follow the directions on this page:
  
  http://surfer.nmr.mgh.harvard.edu/fswiki/FtpFileExchange
  
  in particular, make sure you change directories before trying to put
  
  cheers
  Bruce
  
  On Wed,
  8 Oct 2014,
  Smith,
  Michael wrote:
  
   See attached
  
   On 10/8/14, 10:46 AM, Bruce Fischl fis...@nmr.mgh.harvard.edu
 wrote:
  
   can you send us the details of your ftp command?
   On Wed, 8 Oct 2014, Smith,
   Michael wrote:
  
   Hi Bruce,
  
   Do I not have permission to transfer files to the remote machine? I
  can
   login fine as an anonymous user but when I attempt to use the put
   command
   it says it could not create the file.
  
   On 10/7/14, 7:33 PM, Bruce Fischl fis...@nmr.mgh.harvard.edu
  wrote:
  
   Hi Michael
  
   sure, the T2 can help if you have it (although you'll need to
  download
   some
   updated binaries), but it is impossible to tell from these images
  what
   is
   going on. Looks either the intensity normalization