Re: [Histonet] Epithelial cells
Try a Papanicolau stain. René J. --- On Thu, 3/5/09, Cheryl Crowder ccrow...@vetmed.lsu.edu wrote: From: Cheryl Crowder ccrow...@vetmed.lsu.edu Subject: [Histonet] Epithelial cells To: Histonet histo...@pathology.swmed.edu, Histonet histo...@pathology.swmed.edu, histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Date: Thursday, March 5, 2009, 8:59 PM Hi all - We have a graduate student growing equine epithelial cells in culture. Money is really short. She is wondering if there is a regular special stain for these epithelial cells rather than the more expensive IHC. Any suggestions? Thank you, Cheryl Cheryl Crowder, BA, HTL(ASCP) Chief Technologist Anatomic Pathology Department of Pathobiological Sciences School of Veterinary Medicine Louisiana State University Skip Bertman Drive Baton Rouge, LA 70803 225-578-9734 FAX: 225-578-9720 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] postnatal brain sections using vibratome
hi, i dont have experience on vibratome section at 50 um (for electrophysiology we cut 300). but I performed a lot on microtome or cryostat - frozen sections at 20-40 um. if you want the brain to be harder, perfuse longer and have better post-fixation. unless your antigen is more than fragile and even retrieval does not work. embeding could also be achieved by gelatin or egg yolk. Gelatin is softer, while egg yolk is harder and not transparent (you have to mark the direction). The detach problem could be caused by the wet surface of the brain, and the agarose will fall off. 2009-03-06 TF 发件人： shymaa shawadfy 发送时间： 2009-03-06 10:55:20 收件人： histonet@lists.utsouthwestern.edu 抄送： 主题： [Histonet] postnatal brain sections using vibratome Dear all I am trying to use vibratome 50 祄 thick sections for immunofluorescence using Postnatal day 0 brains. The problem is that brains are very soft and are usually destroyed upon handling and the agarose is separated form the brain. My used protocol was: perfusion with 4 % PFA for 3 min, followed by several hours to overnight post-fixation. Then embedding brains in 2 % low melting agarose and cutting the block on vibratome using low speed. I am thinking to add an overnight 20 % sucrose incubation step following the post-fixation step. Then embed in agarose and continue the normal protocol. May be sucrose will increase the elasticity of the tissue. So what do you think ? Thanks a lot shymaa ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] GFP Antibody
Hi, I use Invitrogen's rabbit (A11122) GFP at 1:1000. Paula Pierce, BS, HTL(ASCP)HT Excalibur Pathology, Inc. 631 N Broadway Ave Moore, OK 73160 405-759-3953 www.excaliburpathology.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] GFP antibody
another option is the antibody from NeoMarkers/Labvision MS-1288. I have used for several years mostly in mouse model but also works in other species and cell lines. have reference if you like + feel free if you have any questions -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of anh2...@med.cornell.edu Sent: Thursday, March 05, 2009 7:08 PM To: Michele Wich; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] GFP antibody The rabbit pAb antibody from Molecular Probes invitrogen works great in frozen and fixed frozen so it might work in paraffin. Worth a shot Andrea --Original Message-- From: Michele Wich Sender: histonet-boun...@lists.utsouthwestern.edu To: histonet@lists.utsouthwestern.edu Sent: Mar 5, 2009 6:53 PM Subject: [Histonet] GFP antibody Can anyone recommend a green fluorescent protein (GFP) antibody that works well in FFPE murine tissue? I have never used this antibody before and am starting from scratch, so any advice is greatly appreciated. This communication is intended solely for the use of the addressee and may contain information that is legally privileged, confidential or exempt from disclosure. If you are not the intended recipient, please note that any dissemination, distribution, or copying of this communication is strictly prohibited. Anyone who receives this message in error should notify the sender immediately and delete it from his or her computer ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain information that is proprietary, confidential, and exempt from disclosure under applicable law. Any unauthorized review, use, disclosure, or distribution is prohibited. If you have received this email in error please notify the sender by return email and delete the original message. Please note, the recipient should check this email and any attachments for the presence of viruses. The organization accepts no liability for any damage caused by any virus transmitted by this email. = ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] histo equipment for sale
Dear Sightdog(?), You did not give your real name (Sightdog doesn't count, unless that IS your real name). You also did not indicate what facility you are with. I'm sure you are on the up and up, but HistoNet Netiquette requires us to identify ourselves and readers would like to know who we are dealing with. What Hospital, Derm. Lab, Path lab, etc. are you affiliated with? Is the equipment located in the lab? Is it still in use? What are you asking, price-wise? Ford M. Royer, MT(ASCP) Minnesota Medical, Inc. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of nilfga...@comcast.net Sent: Wednesday, March 04, 2009 10:08 AM To: histonet@lists.utsouthwestern.edu; histonet-boun...@lists.utsouthwestern.edu Subject: [Histonet] histo equipment for sale Hello histonetters, I have the following, in perfect working condition, items for sale: tissue processor VIP1000 benchtop, just refurbished Shandon 24-3 slide stainer Surgipath PC3001, PC3002 embedding center ducktless hood with new carbon filters hood the a super quite vent system (for outside venting) mini VWR hybridization oven TBS warter bath - like new ONLY, if you are seriously interested, email me at sight...@comcast.net All pieces are located in Chicagoland. Pics available, can ship UPS ground. thanks Sightdog ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] histo equipment for sale
I know this lab - they are very much legitimate. What lab? Jackie M O'Connor wrote: I know this lab - they are very much legitimate. I would suggest anyone interested in the equipment contact them at the email provided, and they will be happy to talk terms and negotiate price one-on-one. Jackie (aka Cadaverdog) Ford Royer fro...@bitstream.net Sent by: histonet-boun...@lists.utsouthwestern.edu 03/06/2009 08:51 AM To nilfga...@comcast.net, sight...@comcast.net cc histonet@lists.utsouthwestern.edu, histonet-boun...@lists.utsouthwestern.edu Subject RE: [Histonet] histo equipment for sale Dear Sightdog(?), You did not give your real name (Sightdog doesn't count, unless that IS your real name). You also did not indicate what facility you are with. I'm sure you are on the up and up, but HistoNet Netiquette requires us to identify ourselves and readers would like to know who we are dealing with. What Hospital, Derm. Lab, Path lab, etc. are you affiliated with? Is the equipment located in the lab? Is it still in use? What are you asking, price-wise? Ford M. Royer, MT(ASCP) Minnesota Medical, Inc. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of nilfga...@comcast.net Sent: Wednesday, March 04, 2009 10:08 AM To: histonet@lists.utsouthwestern.edu; histonet-boun...@lists.utsouthwestern.edu Subject: [Histonet] histo equipment for sale Hello histonetters, I have the following, in perfect working condition, items for sale: tissue processor VIP1000 benchtop, just refurbished Shandon 24-3 slide stainer Surgipath PC3001, PC3002 embedding center ducktless hood with new carbon filters hood the a super quite vent system (for outside venting) mini VWR hybridization oven TBS warter bath - like new ONLY, if you are seriously interested, email me at sight...@comcast.net All pieces are located in Chicagoland. Pics available, can ship UPS ground. thanks Sightdog ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] CD31 on rat brain
Just wonder anyone has a suggestion of antibody that works on rat brain? Frozen section and parafin sections? It would be great if this also works on mice. 2009-03-06 TF 发件人： Colleen Forster 发送时间： 2009-03-06 00:57:29 收件人： tifei 抄送： iskaliora; histonet; ΕΛΕΝΗ ΚΟΝΣΟΛΑΚΗ 主题： Re: [Histonet] staining brain vessels You have to have a specific CD31 to work on mouse brain. Very few of them do. Colleen Forster TF wrote: hi, CD31 works great in my section alpha-SMA also works another way is to perfuse the brain with BSA-rhodamine. you will get the fluorescence without the need of staining. 2009-03-05 TF 发件人： iskaliora 发送时间： 2009-03-05 18:49:11 收件人： histonet 抄送： ΕΛΕΝΗ ΚΟΝΣΟΛΑΚΗ 主题： [Histonet] staining brain vessels I was wondering if anybody might have an idea with the following problem we are experiencing: we want to stain for blood vessels in sections of mouse brain. Our experimental tissues have been fixed overnight in 4% paraformaldehyde and have been sitting in PBS since. We have tried staining with antibodies against desmin, SMA, and collagen but we get NO specific signal. We recently tried a non-fixed mouse brain and got desmin to work immediately. The problem is that we need to use the fixed brains because they are our experimental model and it would take too long (2 years to be exact) to generate the same samples. If anybody has come across such a problem before, or has a specific protocol for vessels that works on PFA fixed brain, we would appreciate the suggestions! thanks in advance! Irini - Irini Skaliora, PhD Investigator C᾽ (Assistant Professor) Developmental Biology Division Biomedical Research Foundation of the Academy of Athens (BRFAA) Soranou Efessiou 4 Athens 11527 tel. +30-210-6597203 (office) tel. +30-210-6597482 (lab) fax. +30-210-6597545 email: iskali...@bioacademy.gr ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: GFP antibody
We have had success staining murine paraffin sections using Rockland goat anti-GFP antibody. You'll need to optimize it to your laboratory conditions trying with either antigen retrieval (citrate buffer pH 6.0) or Proteinase K. Make sure you have tissue with known positive GFP expression and a wild type (negative) animal for controls. As for whether processing destroys the GFP in the sample, it will render the protein non-fluorescent. Fixation alone can do that. But the protein should still be there, even if it is not glowing. Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] CD31 on rat brain
For mouse (specifically) have used CD31 from BD catalog number 550274. Its a rat ant-mouse (Clone MEC13.3). can supply more info if need. The problem I have found is finding good control material for this marker. especially since my only source tends to be the investigators that provide me the samples to begin with. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of TF Sent: Friday, March 06, 2009 10:23 AM To: Colleen Forster Cc: histonet; ΕΛΕΝΗ ΚΟΝΣΟΛΑΚΗ Subject: [Histonet] CD31 on rat brain Just wonder anyone has a suggestion of antibody that works on rat brain? Frozen section and parafin sections? It would be great if this also works on mice. 2009-03-06 TF 发件人： Colleen Forster 发送时间： 2009-03-06 00:57:29 收件人： tifei 抄送： iskaliora; histonet; ΕΛΕΝΗ ΚΟΝΣΟΛΑΚΗ 主题： Re: [Histonet] staining brain vessels You have to have a specific CD31 to work on mouse brain. Very few of them do. Colleen Forster TF wrote: hi, CD31 works great in my section alpha-SMA also works another way is to perfuse the brain with BSA-rhodamine. you will get the fluorescence without the need of staining. 2009-03-05 TF 发件人： iskaliora 发送时间： 2009-03-05 18:49:11 收件人： histonet 抄送： ΕΛΕΝΗ ΚΟΝΣΟΛΑΚΗ 主题： [Histonet] staining brain vessels I was wondering if anybody might have an idea with the following problem we are experiencing: we want to stain for blood vessels in sections of mouse brain. Our experimental tissues have been fixed overnight in 4% paraformaldehyde and have been sitting in PBS since. We have tried staining with antibodies against desmin, SMA, and collagen but we get NO specific signal. We recently tried a non-fixed mouse brain and got desmin to work immediately. The problem is that we need to use the fixed brains because they are our experimental model and it would take too long (2 years to be exact) to generate the same samples. If anybody has come across such a problem before, or has a specific protocol for vessels that works on PFA fixed brain, we would appreciate the suggestions! thanks in advance! Irini - Irini Skaliora, PhD Investigator C᾽ (Assistant Professor) Developmental Biology Division Biomedical Research Foundation of the Academy of Athens (BRFAA) Soranou Efessiou 4 Athens 11527 tel. +30-210-6597203 (office) tel. +30-210-6597482 (lab) fax. +30-210-6597545 email: iskali...@bioacademy.gr ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain information that is proprietary, confidential, and exempt from disclosure under applicable law. Any unauthorized review, use, disclosure, or distribution is prohibited. If you have received this email in error please notify the sender by return email and delete the original message. Please note, the recipient should check this email and any attachments for the presence of viruses. The organization accepts no liability for any damage caused by any virus transmitted by this email. = ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] re: staining of elastin fibers (resend)
This is a resend of a previous message that seems to have been chopped to pieces in cyberspace. Hope this one comes through better: I prefer to use iron gallein elastin stain for demonstrating elasti fibers. It is simple to make up and gives good contrast compared to some of the other stains. Nuclei stain dark blue, elastin fibers black, everything else is pink or light purple. The protocol can be found in Humanson's Animal tissue techniques and the original article is by Churukian and Schenk (Stain Tech., 1976). I can provide a protocol in .doc format I can send, along with any notes, if you don't have access to either source. Current suppliers of gallein are Fisher Scientific, Spectrum Chemicals, and ArtChemicals.com. Spectrum has it cheapest. Good luck. -Matt - Matthew T. Close Lehigh University Department of Biological Sciences ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] CD31 on rat brain
I would guess that there are problems with the antibodies you are using. SMA is usually a very robust antigen for IHC, if you're not getting any staining you're probably using the wrong antibody. You need an antibody whose specs indicate that it works on mouse, and that it works on fixed tissue. You also don't give details on your staining protocol - it could be a technical issue with that as well. To find proper antibodies, I have found the Biocompare website to be an excellent place to look. Jean-Martin Lapointe AccelLAB Inc -- Message: 11 Date: Sat, 7 Mar 2009 00:44:32 +0800 From: TF ti...@foxmail.com Subject: Re: RE: [Histonet] CD31 on rat brain [Histonet] staining brain vessels I was wondering if anybody might have an idea with the following problem we are experiencing: we want to stain for blood vessels in sections of mouse brain. Our experimental tissues have been fixed overnight in 4% paraformaldehyde and have been sitting in PBS since. We have tried staining with antibodies against desmin, SMA, and collagen but we get NO specific signal. We recently tried a non-fixed mouse brain and got desmin to work immediately. The problem is that we need to use the fixed brains because they are our experimental model and it would take too long (2 years to be exact) to generate the same samples. If anybody has come across such a problem before, or has a specific protocol for vessels that works on PFA fixed brain, we would appreciate the suggestions! thanks in advance! Irini - Irini Skaliora, PhD Investigator Cá¾½ (Assistant Professor) Developmental Biology Division Biomedical Research Foundation of the Academy of Athens (BRFAA) Soranou Efessiou 4 Athens 11527 tel. +30-210-6597203 (office) tel. +30-210-6597482 (lab) fax. +30-210-6597545 email: iskali...@bioacademy.gr ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Histology for Zebrafish
Hi, I need to work on zebrafish for the first time and would like to get some general protocols for fixation and IHC staining. My investigator used 4% PFA for 2 hrs at RT for a whole zebrafish and sections didn't come out right (falling off not preserved well). I am going to increase the time and also try 10% formalin over night. Please let me know if formalin works ok for IHC later. Thank you. Judy ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Have a person on 3rd Shift ???
I have posted before, but would like some more feed back on this issue. Thanks to everyone who has already given me feedback on this! I would like to start working a person on third shift to try and relieve some stress for 1st shift. Is there any one who would like to give me their thoughts and opinions on this topic. Did it save money?, Did it relieve stress?? Was it easy to staff? And anything else that it may have helped or hindered. Andria B Evans, HTL(ASCP)CM Anatomic Pathology York Hospital 1001 S. George Street York, PA 17405 717-851-5006 You can learn a lot more from listening than you can from talking. Find someone with whom you don't agree in the slightest and ask them to explain themselves at length. Then take a seat, shut your mouth, and don't argue back. It's physically impossible to listen with your mouth open. -John Moe Maturity is accepting imperfections. CONFIDENTIALITY NOTICE: This email may contain confidential health information that is legally privileged. This information is intended for the use of the named recipient(s). The authorized recipient of this information is prohibited from disclosing this information to any party unless required to do so by law or regulation and is required to destroy the information after its stated need has been fulfilled. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or action taken in reliance on the contents of this email is strictly prohibited. If you receive this e-mail message in error, please notify the sender immediately to arrange disposition of the information. __ This e-mail has been scanned by MCI Managed Email Content Service, using Skeptic(tm) technology powered by MessageLabs. For more information on MCI's Managed Email Content Service, visit http://www.mci.com. __ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Histology for Zebrafish
Have you checked out the ton of protocols (including histo) available at ZFIN online: http://zfin.org/zf_info/zfbook/zfbk.html Hi, I need to work on zebrafish for the first time and would like to get some general protocols for fixation and IHC staining. My investigator used 4% PFA for 2 hrs at RT for a whole zebrafish and sections didn't come out right (falling off not preserved well). I am going to increase the time and also try 10% formalin over night. Please let me know if formalin works ok for IHC later. Thank you. Judy ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] HT exam
Hi MaryAnn, I am also about to take my HTL exam. The website has all the information you are looking for. I've been through these testing facilities when I took my GRE. Make sure you have at least 2 forms of I.D. with you, do not bring a cell phone, etc (all this is on the ASCP website). You will not get any results right away. I don't remember if you can go back and answer missed questions, but I doubt it. The way it works is that there is a pool of about 500+ questions worth at most 900 points. Each question is worth a certain amount of points based on the difficulty of the question. If you get a question correct, the next one will be harder. If you get it wrong, the next will be easier. You will answer 100 questions. Surf through the website and you will find all the answers you seek. (Click on the HT link from this page and a pdf will come up with the testing breakdown) http://www.ascp.org/FunctionalNavigation/certification/GetCertified/Examinat ionContentGuidelines.aspx Katelin Lester Cutting Edge Histology Services, LLC (503) 443-2157 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of MaryAnn Dixon Sent: Friday, March 06, 2009 10:53 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HT exam Hi everyone out there in Histoland, Well, I'm getting close to my HT exam date and was wondering if any of you had recently taken the exam. I can't find any info on the test itself as far as if it is weighted or just a straight 150 questions. Can you flag questions and go back? Do you know when you submit the test if you have passed or not? I've been told in the past that the computer would just shut off and a screen would come up pass or fail. Anyway, I have been focusing on Frieda Carson as well as the AFIP manual. Any suggestions for a successful passing score? I think you have to get a minimum of 400. Should I be concerned with microwave special stains? I would think the test is more old school. Any helpful hints would be truly appreciated. Thanks! MaryAnn Dixon BS Biological Scientist Anatomic Pathology UF Veterinary Medical Center (352) 352-2235 Ext. 4517 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Morgue responsibilities
Hi everyone. Happy Friday! We are in the process of reviewing our morgue policies, and the question about ownership has come up. How many of you are responsible for maintaining the morgue? Some hospitals I know have Security in charge, while others say Nursing or the Lab. Who is responsible for repairs, ordering new stretchers, hoists and refrigeration units? If the refrigeration goes down, what is your back-up plan? Do you have an agreement with another hospital, or Medical Examiner? Do you use refrigerated trucks? What is in your Disaster Policy when extra body storage is needed? Thanks in advance for all of your responses. Toni Rathborne Pathology Supervisor Somerset Medical Center 110 Rehill Ave. Somerville,NJ 08876 908-595-2367 CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Murine Pathologist Needed ASAP in MA area
Dear Histonetters! I was wondering if there are any or someone knows a veterinary pathologist who has experience working with mouse tissues and possibly Xenograft models? The pathologist we were outsourcing to has moved to a different state. We need one in Boston or nearby Massachusetts area. Any information would be greatly appreciated. Igor Deyneko. Infinity Pharmaceuticals Cambridge, MA 02139 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] HT exam
MaryAnn, The ASCP uses Computer Adaptive Testing (CAT) for the certification exams. As another person responded your questions will be based on the response to the previous questions. If you get the answer right subsequent questions asked will be of increasing difficulty and worth more points. The maximum score is 999. You need 400 to pass the exam. It is wise to answer each questions to the best of your ability so that you will receive questions with higher point values. You will have an opportunity to review all or some of your responses before you submit the exam. At the conclusion of the exam you will get a message on the screen to indicate that you have passed or failed. You will receive your final score in the mail in about 10 days. Applicants that are not successful will receive a breakdown of the scores by category (fixation, processing, microtomy, staining, and lab operations). Successful applicants just receive the total score. You will have 2.5 hours to answer the 100 questions. 40-50% of the exam is based on staining so that is an area that you will want to concentrate on, but not at the exclusion of the others. The test questions have been updated so don't rely on receiving old school questions. Also remember that since 2001 cytology preparation is included. You are also responsible for the basic theory of IHC, including antigen retrieval, controls, and antibody preparation. The following link will direct you to an explanation of CAT. http://www.ascp.org/FunctionalNavigation/certification/GetCertified/ComputerAdaptiveTestingCAT.aspx This link will direct you to the examination guidelines for the HT/HTL exams. On the last page is a list of stains to concentrate on. http://www.ascp.org/pdf/ Jennifer MacDonald Education Coordinator, Histotechnician Training Program Mt. San Antonio College 1100 N. Grand Ave. Walnut, CA 91789 (909) 594-5611 ext. 4884 jmacdon...@mtsac.edu MaryAnn Dixon dix...@vetmed.ufl.edu Sent by: histonet-boun...@lists.utsouthwestern.edu 03/06/2009 10:53 AM To histonet@lists.utsouthwestern.edu cc Subject [Histonet] HT exam Hi everyone out there in Histoland, Well, I'm getting close to my HT exam date and was wondering if any of you had recently taken the exam. I can't find any info on the test itself as far as if it is weighted or just a straight 150 questions. Can you flag questions and go back? Do you know when you submit the test if you have passed or not? I've been told in the past that the computer would just shut off and a screen would come up pass or fail. Anyway, I have been focusing on Frieda Carson as well as the AFIP manual. Any suggestions for a successful passing score? I think you have to get a minimum of 400. Should I be concerned with microwave special stains? I would think the test is more old school. Any helpful hints would be truly appreciated. Thanks! MaryAnn Dixon BS Biological Scientist Anatomic Pathology UF Veterinary Medical Center (352) 352-2235 Ext. 4517 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] HT exam
http://www.ascp.org/pdf/HTHTLSummaryofStainsforComputerExamination.aspx Sorry. The link was incomplete. Jennifer MacDonald jmacdon...@mtsac.edu Sent by: histonet-boun...@lists.utsouthwestern.edu 03/06/2009 01:59 PM To MaryAnn Dixon dix...@vetmed.ufl.edu cc histonet@lists.utsouthwestern.edu, histonet-boun...@lists.utsouthwestern.edu Subject Re: [Histonet] HT exam MaryAnn, The ASCP uses Computer Adaptive Testing (CAT) for the certification exams. As another person responded your questions will be based on the response to the previous questions. If you get the answer right subsequent questions asked will be of increasing difficulty and worth more points. The maximum score is 999. You need 400 to pass the exam. It is wise to answer each questions to the best of your ability so that you will receive questions with higher point values. You will have an opportunity to review all or some of your responses before you submit the exam. At the conclusion of the exam you will get a message on the screen to indicate that you have passed or failed. You will receive your final score in the mail in about 10 days. Applicants that are not successful will receive a breakdown of the scores by category (fixation, processing, microtomy, staining, and lab operations). Successful applicants just receive the total score. You will have 2.5 hours to answer the 100 questions. 40-50% of the exam is based on staining so that is an area that you will want to concentrate on, but not at the exclusion of the others. The test questions have been updated so don't rely on receiving old school questions. Also remember that since 2001 cytology preparation is included. You are also responsible for the basic theory of IHC, including antigen retrieval, controls, and antibody preparation. The following link will direct you to an explanation of CAT. http://www.ascp.org/FunctionalNavigation/certification/GetCertified/ComputerAdaptiveTestingCAT.aspx This link will direct you to the examination guidelines for the HT/HTL exams. On the last page is a list of stains to concentrate on. http://www.ascp.org/pdf/ Jennifer MacDonald Education Coordinator, Histotechnician Training Program Mt. San Antonio College 1100 N. Grand Ave. Walnut, CA 91789 (909) 594-5611 ext. 4884 jmacdon...@mtsac.edu MaryAnn Dixon dix...@vetmed.ufl.edu Sent by: histonet-boun...@lists.utsouthwestern.edu 03/06/2009 10:53 AM To histonet@lists.utsouthwestern.edu cc Subject [Histonet] HT exam Hi everyone out there in Histoland, Well, I'm getting close to my HT exam date and was wondering if any of you had recently taken the exam. I can't find any info on the test itself as far as if it is weighted or just a straight 150 questions. Can you flag questions and go back? Do you know when you submit the test if you have passed or not? I've been told in the past that the computer would just shut off and a screen would come up pass or fail. Anyway, I have been focusing on Frieda Carson as well as the AFIP manual. Any suggestions for a successful passing score? I think you have to get a minimum of 400. Should I be concerned with microwave special stains? I would think the test is more old school. Any helpful hints would be truly appreciated. Thanks! MaryAnn Dixon BS Biological Scientist Anatomic Pathology UF Veterinary Medical Center (352) 352-2235 Ext. 4517 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Masson's trichrome stain
I am having a problem with Biebrich Scarlet. I am attempting to do a Masson's stain on some mouse heart tissue. Instead of the strikingly bright red of the muscle tissue I am getting a purple color instead. Everything else looks fine. The hearts are fixed in Penfix and are embedded in paraffin. We make the solution up fresh for each run. I am using Sigma's Ponceau BS (CI 26905). Any ideas? Katherine Walters Histology Director Central Microscopy Research Facilities 85 Eckstein Medical Research Building University of Iowa Iowa City, Iowa 52242-1101 katherine-walt...@uiowa.edu www.uiowa.edu/~cemrf Notice: This UI Health Care e-mail (including attachments) is covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521, is confidential and may be legally privileged. If you are not the intended recipient, you are hereby notified that any retention, dissemination, distribution, or copying of this communication is strictly prohibited. Please reply to the sender that you have received the message in error, then delete it. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Morgue responsibilities
Pathology/Histology is responsible for the autopsy suite, providing the autopsy service, stocking the morgue. Nursing is responsible for the delivery of patients 24/7 to the morgue. Histology is responsible for releasing patients on day shift and arranging the autopsy if one is requested. We have an autopsy assistant who operates our morgue and works in histology when she is not busy providing service to the morgue. Evenings, nights and weekends the nursing service releases the patients. We are responsible for repairs to the autopsy suite. Nursing provides the transport carts. In case of a disaster, another room in the hospital is designated as the temporary morgue. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 1 Children's WaySlot 820 Little Rock, AR 72202 phone 501.364.4240 fax501.364.3155 visit us on the web at:www.archildrens.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Friday, March 06, 2009 1:51 PM To: Histonet_Listserv (E-mail) Subject: [Histonet] Morgue responsibilities Hi everyone. Happy Friday! We are in the process of reviewing our morgue policies, and the question about ownership has come up. How many of you are responsible for maintaining the morgue? Some hospitals I know have Security in charge, while others say Nursing or the Lab. Who is responsible for repairs, ordering new stretchers, hoists and refrigeration units? If the refrigeration goes down, what is your back-up plan? Do you have an agreement with another hospital, or Medical Examiner? Do you use refrigerated trucks? What is in your Disaster Policy when extra body storage is needed? Thanks in advance for all of your responses. Toni Rathborne Pathology Supervisor Somerset Medical Center 110 Rehill Ave. Somerville,NJ 08876 908-595-2367 CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. ** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
