[Histonet] slide disposal
Hi everyone! Thank you for all the great responses to my last question about metal vs. plastic molds. I have another question being debated however, How to dispose of slides once the required time is up (10 years for us). We have put the slide in sharps containers and then into biohazard, but are they really bioharzard, the slides only contain the patients accession number - no personal ID on the slides? How do you guys dispose of your slides when space and money are an issue? Thank you Sharon Campbell, HTL(ASCP)CM, BSBM Histology Supervisor Celligent Diagnostics, LLC Formerly Pathology Associates Services 101 East W.T. Harris Blvd, Suite 1212 Charlotte, NC 28262 800-524-6779 ext. 104 704-970-3304 Direct Line shar...@celligent.net ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Cutting angle?
I have a refurbished Reichert-Jung 2030, but with a knife holder from a newer model (so I'm told by the refurbisher). I use 0-1 degree angle. Merced --On Tuesday, March 10, 2009 4:15 PM -0700 Va Paula Sicurello vapat...@yahoo.com wrote: Hello Listers, I have inherited a Reichert-Jung 2030 microtome. The knife holder is a bit funky and hard to set the clearance angle. What angle do y'all use? I'm trying to get 4 or 5 degrees. Thanks, Paula :-) Paula Sicurello VA Medical Center San Diego Veterans Medical Research Foundation (VMRF) Core Research Imaging Center 3350 La Jolla Village Dr., MC151 San Diego, CA 92161 858-552-8585 x2397 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Merced M Leiker Research Technician II 354 Biomedical Research Building School of Medicine and Biomedical Sciences State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 Ph: (716) 829-6033 Fx: (716) 829-2725 No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] incubator oven
Hi All, I desperately need to get a new incubator oven for my histology lab. It seems as if most ovens are now convection ovens. Since my old oven is not convection I am just concerned that the constant air movement will some how affect the tissue slides in the way the paraffin in melted before the processing of the slides. I will be using this oven for strictly melting paraffin containers to have ready for embedding center and for baking of slides to melt paraffin prior to processing. Has anyone had any concerns or issues with these new convection ovens or can they recommend a decent oven to buy. it just needs to be a medium sized oven. Thanks in advance. Kris kalleberg kristopher.kalleb...@unilever.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] slide disposal
We have a broken glass waste container we put ours in for pick up and disposal (don't deal with non-animal patients, so not an issue). -M --On Wednesday, March 11, 2009 4:56 AM -0400 Sharon Campbell shar...@celligent.net wrote: Hi everyone! Thank you for all the great responses to my last question about metal vs. plastic molds. I have another question being debated however, How to dispose of slides once the required time is up (10 years for us). We have put the slide in sharps containers and then into biohazard, but are they really bioharzard, the slides only contain the patients accession number - no personal ID on the slides? How do you guys dispose of your slides when space and money are an issue? Thank you Sharon Campbell, HTL(ASCP)CM, BSBM Histology Supervisor Celligent Diagnostics, LLC Formerly Pathology Associates Services 101 East W.T. Harris Blvd, Suite 1212 Charlotte, NC 28262 800-524-6779 ext. 104 704-970-3304 Direct Line shar...@celligent.net ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Merced M Leiker Research Technician II 354 Biomedical Research Building School of Medicine and Biomedical Sciences State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 Ph: (716) 829-6033 Fx: (716) 829-2725 No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] slide disposal
Old slides, after all the processing they have endured are not hazardous (from the contagious/disease point of view) any more. They are hazardous from the mechanical (potential physical injury point of view) and it is more than enough to dispose of them in sharps containers. Word of caution, just do not dump the slides on the sharp containers because they will occupy much more space (and will require more sharp containers) as if you try to place them in a good arrangement. Do not succumb to the temptation of making noise with the old slides as yiu dispose of them! René J. --- On Wed, 3/11/09, Sharon Campbell shar...@celligent.net wrote: From: Sharon Campbell shar...@celligent.net Subject: [Histonet] slide disposal To: histonet@lists.utsouthwestern.edu Date: Wednesday, March 11, 2009, 4:56 AM Hi everyone! Thank you for all the great responses to my last question about metal vs. plastic molds. I have another question being debated however, How to dispose of slides once the required time is up (10 years for us). We have put the slide in sharps containers and then into biohazard, but are they really bioharzard, the slides only contain the patients accession number - no personal ID on the slides? How do you guys dispose of your slides when space and money are an issue? Thank you Sharon Campbell, HTL(ASCP)CM, BSBM Histology Supervisor Celligent Diagnostics, LLC Formerly Pathology Associates Services 101 East W.T. Harris Blvd, Suite 1212 Charlotte, NC 28262 800-524-6779 ext. 104 704-970-3304 Direct Line shar...@celligent.net ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] incubator oven
Usually all ovens (new and old are of the convection type) and the air circulation will improve the effect of heat over the slides. René J. --- On Wed, 3/11/09, Kalleberg, Kristopher kristopher.kalleb...@unilever.com wrote: From: Kalleberg, Kristopher kristopher.kalleb...@unilever.com Subject: [Histonet] incubator oven To: histonet@lists.utsouthwestern.edu Date: Wednesday, March 11, 2009, 9:40 AM Hi All, I desperately need to get a new incubator oven for my histology lab. It seems as if most ovens are now convection ovens. Since my old oven is not convection I am just concerned that the constant air movement will some how affect the tissue slides in the way the paraffin in melted before the processing of the slides. I will be using this oven for strictly melting paraffin containers to have ready for embedding center and for baking of slides to melt paraffin prior to processing. Has anyone had any concerns or issues with these new convection ovens or can they recommend a decent oven to buy. it just needs to be a medium sized oven. Thanks in advance. Kris kalleberg kristopher.kalleb...@unilever.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] IF on frozen sections
Hi everyone, Does anyone have any experience in IF on mouse embryo frozen sections? Vanessa ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: CAP regs on Histonet
For ease of reference, if you are asking, replying, or quoting anything pertaining to CAP regs, PLEASE give the CAP checklist number that contains the information being discussed. For those of us trying to keep current, it makes it so much easier to check our own records. Just a suggestion - Terri Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital and Medical Center 1648 Huntingdon Pike Meadowbrook, PA 19046 (215) 938-3676 phone (215) 938-3689 fax - CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] CPT Coding question 88342 PIN 4 cocktail
Disclaimer: I do not consider myself to be a coding expert. Take my opinions with a grain of salt. I've never seen coding for softening of keratin therefore I don't see how you could charge for it. This is not decalcification and should not be represented as such. Until such time as a code appears (don't hold your breath) the KOH or whatever technique you employ is for your convenience to make a block more cuttable (is that a word?). There is no rule that technical and professional charges must be the same. They are reimbursed differently, sometimes the technical having a higher reimbursement than the professional. You are free to set your charges for each where you see fit. I believe that your charges for prostate IHC have to be justified by the pathology. In your example, some of those cores may be clearly benign and there would be no basis for charging IHC for eight cores if only one or two contain the lesion. In your case, by having four cores in one block, you benefit by keeping your expenses down however I do believe that your IHC charges should (must?) be based on the location of the lesion under study. It would seem to me that charging for IHC on eight cores when the report describes a lesion in only one is asking for trouble You are correct that you can charge 88342 x 3 for the PIN 4 markers as each is separate and can be visualized distinctly from one another. Vinnie Della Speranza Manager for Anatomic Pathology Services Medical University of South Carolina 165 Ashley Avenue Suite 309 Charleston, South Carolina 29425 Tel: (843) 792-6353 Fax: (843) 792-8974 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Shea's Sent: Tuesday, March 10, 2009 9:09 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CPT Coding question 88342 PIN 4 cocktail We started staining prostate needle bxs w/ PIN 4 (triple stain). 1. My understanding is that we can bill for 88342 x 3 (per specimen) if a comment is made on the results of the nuclear staining and cytoplasmic staining of the DAB and the staining of the Vulcan red. The key is documentation in the report. This is simple to understand when you rec'd 1 specimen/container, but we rec'd: A. Right prostate Bx - Red stained bx: apex Green stained bx: base Blue stained bx: midgland Yellow stained bx: transition submitted in one Cassette A. B. Left prostate Bx - Red stained bx: apex Green stained bx: base Blue stained bx: midgland Yellow stained bx: transition submitted in one Cassette B. 2. If the pathologist needed this stain on every specimen, it would be 8 separate identifiable specimens x 3 (separate identifiable stains in the cocktail) , therefore88342 x 24, even though it is only 2 blocks. 3. If the pathologist was only interested in this stain on on the Apex in A and B it would be 2 specimens x 3, therefore 88342 x 6 for the same 2 blocks (even though it stains all 8 specimens, only 2 of the 8 are in need of this stain). Another words, we can't charge the technical component until we find out how many specimens the pathologist is looking at in a slide (professional component), even though it is the same amount of work and reagents. Does this sound right? 4. Is the technical charge always the same as the professional charge? Another unrelated question - We can bill for decalcification, but is there a billing code for KOH in the same manner to soften and treat toenails before processing? It is documented in the Path report. I know this has been discussed in the archives, but there seems to be conflicting opinions. Do we know for sure? Thanks ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] MOLDS AND SLIDE DISPOSAL
Metal molds vs Disposable molds We too have chosen metal molds. We clean them by placing them in a random processing basket and running an extended purge cycle with xylene and alcohol. It has worked very well for us. Slide disposal: I believe the CAP guidelines from 2005 state that slides should be kept for a minimum of 20 years. I don't know if this has been recently revised. Here in Alberta, Canada, the retention guidelines for slides is 30 years.(College of Physicians and Surgeons of Alberta) We also package into plastic pails to be picked up by a disposal company. They are never put into a regular dumpster as there is definite danger of slide breakage and potential injury to anyone who comes in contact with them. I realize that this is an added expense however, with a longer retention time, the slides are not discarded as often. Karen J Kay, MLT Pathology Supervisor Chinook Health Laboratory - Chinook Regional Hospital 960 - 19 st South,Lethbridge, Alberta,CANADA (403) 388 - 6061 (Phone) Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sharon Campbell Sent: Wednesday, March 11, 2009 3:57 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] slide disposal Hi everyone! Thank you for all the great responses to my last question about metal vs. plastic molds. I have another question being debated however, How to dispose of slides once the required time is up (10 years for us). We have put the slide in sharps containers and then into biohazard, but are they really bioharzard, the slides only contain the patients accession number - no personal ID on the slides? How do you guys dispose of your slides when space and money are an issue? Thank you Sharon Campbell, HTL(ASCP)CM, BSBM Histology Supervisor Celligent Diagnostics, LLC Formerly Pathology Associates Services 101 East W.T. Harris Blvd, Suite 1212 Charlotte, NC 28262 800-524-6779 ext. 104 704-970-3304 Direct Line shar...@celligent.net - This communication is intended for the use of the recipient to which it is addressed, and may contain confidential, personal and or privileged information. Please contact us immediately if you are not the intended recipient. Do not copy, distribute or take action relying on it. Any communication received in error, or subsequent reply, should be deleted or destroyed. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] incubator oven
Hi Kris, I think that a smallish lab oven would suffice. If all you are doing is melting paraffins I think any of the ones from the major vendors (Fisher, VWR, etc.) would be good enough. I do believe that those are still just ovens and don't have the air currents. Paula Sicurello VA Medical Center San Diego Veterans Medical Research Foundation (VMRF) Core Research Imaging Center 3350 La Jolla Village Dr., MC151 San Diego, CA 92161 858-552-8585 x2397 --- On Wed, 3/11/09, Kalleberg, Kristopher kristopher.kalleb...@unilever.com wrote: From: Kalleberg, Kristopher kristopher.kalleb...@unilever.com Subject: [Histonet] incubator oven To: histonet@lists.utsouthwestern.edu Date: Wednesday, March 11, 2009, 1:40 PM Hi All, I desperately need to get a new incubator oven for my histology lab. It seems as if most ovens are now convection ovens. Since my old oven is not convection I am just concerned that the constant air movement will some how affect the tissue slides in the way the paraffin in melted before the processing of the slides. I will be using this oven for strictly melting paraffin containers to have ready for embedding center and for baking of slides to melt paraffin prior to processing. Has anyone had any concerns or issues with these new convection ovens or can they recommend a decent oven to buy. it just needs to be a medium sized oven. Thanks in advance. Kris kalleberg kristopher.kalleb...@unilever.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] slide disposal
We use the same company that takes away our alcohol waste. They pulverize the slides for us. There is a company in WI that makes a Slydeater that you can rent for one month to pulverize your slides. I am looking into that aspect. Joyce Cline, H.T. (ASCP) Technical Specialist Hagerstown Medical Lab. 301-665-4980 fax 301-665-4941 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sharon Campbell Sent: Wednesday, March 11, 2009 4:57 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] slide disposal Hi everyone! Thank you for all the great responses to my last question about metal vs. plastic molds. I have another question being debated however, How to dispose of slides once the required time is up (10 years for us). We have put the slide in sharps containers and then into biohazard, but are they really bioharzard, the slides only contain the patients accession number - no personal ID on the slides? How do you guys dispose of your slides when space and money are an issue? Thank you Sharon Campbell, HTL(ASCP)CM, BSBM Histology Supervisor Celligent Diagnostics, LLC Formerly Pathology Associates Services 101 East W.T. Harris Blvd, Suite 1212 Charlotte, NC 28262 800-524-6779 ext. 104 704-970-3304 Direct Line shar...@celligent.net ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet * CONFIDENTIALITY NOTICE * This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Need Advice on Low Temp Tissue Processing
Sean: Considering that the van't Hoff coefficient determines a metabolic coefficient increase/reduction every 10ºC (Q10) I would increase the processing period one time every 10ºc between the temperature your protocol was designed to work at and the new temperature you are going to use now. Lets say that you fine tuned your protocol for 35 ºC and now you will be working at 4ºC which is a almost 30ºC less = 10ºC x 3 If a given step at 35ºC was set at 1 hour, I would use now 3 hours for the same step. René J. --- On Wed, 3/11/09, Sean McBride smcbr...@cs.cmu.edu wrote: From: Sean McBride smcbr...@cs.cmu.edu Subject: [Histonet] Need Advice on Low Temp Tissue Processing To: histonet@lists.utsouthwestern.edu Date: Wednesday, March 11, 2009, 12:30 PM Hi folks, I have a rabbit cranial defect study in which the implant material is sensitive to 100% ethanol at temperatures 20*C. As I cannot use my my Sakura tissue processor for the specimens (min operating temp=35*C), I plan to process them by hand @ 4*C in a cold room. My intuition tells me that at lower temperatures, the processing times in each solution should be extended by a percentage, but by how much, I have not an idea. Does anyone have any experience or ideas with regards to low temperature tissue processing times? Best regards, ~Sean McBride Bone Tissue Engineering Center Carnegie Mellon Research Institute Suite 4311 700 Technology Drive Pittsburgh, PA 15219-3124 412-268-8275 (o) 412-901-7540 (m) 412-268-8641 (fax) smcbr...@cs.cmu.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] paraffin oil-clearing agent
hi everyone, last week i had aquestion regarding xylene substitutes and Vector Blue compatibility. i came across an article saying use of paraffin oil as substitute for xylene... can paraffin oil be used as clearing agent ? _ How fun is this? IMing with Windows Live Messenger just got better. http://www.microsoft.com/india/windows/windowslive/messenger.aspx___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Compromised specimens
When our histology lab receives a compromised specimen (not labeled with patient's name, wrong DOB, no label whatsoever, etc) we have the offending party correct the error and fill out an accountability form accepting full responsibility for the identification of the specimen. We then scan the accountability form into the patient's medical record and include the information in the specimen comments section of the pathology record. This information does not print on the official pathology report. My question - should the pathologist include in his/her report the compromised specimen information? Presently, on our clinical lab reports and cytology reports it is included - simply saying Compromised Specimen. Our pathologists historically have not wanted to include this in the official pathology report and I feel that it should be in there - sort of a cover your butt addendum. I'm curious what other hospitals do!! Thanks in advance, Lynne A. Bell, HT (ASCP) Lead Histologist Central Vermont Medical Center 130 Fisher Road Barre, VT 05641 802-371-4923 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: RE: [Histonet] slide disposal
we treat with SHARPS + Biological Hazardous 2009-03-12 TF 发件人： Mike Pence 发送时间： 2009-03-11 21:29:29 收件人： Sharon Campbell; histonet@lists.utsouthwestern.edu 抄送： 主题： RE: [Histonet] slide disposal All of our slides are put into buckets and placed in the regular dumpster as long as there is no patient names on the slides. We don't even treat them as sharps. Mike -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sharon Campbell Sent: Wednesday, March 11, 2009 3:57 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] slide disposal Hi everyone! Thank you for all the great responses to my last question about metal vs. plastic molds. I have another question being debated however, How to dispose of slides once the required time is up (10 years for us). We have put the slide in sharps containers and then into biohazard, but are they really bioharzard, the slides only contain the patients accession number - no personal ID on the slides? How do you guys dispose of your slides when space and money are an issue? Thank you Sharon Campbell, HTL(ASCP)CM, BSBM Histology Supervisor Celligent Diagnostics, LLC Formerly Pathology Associates Services 101 East W.T. Harris Blvd, Suite 1212 Charlotte, NC 28262 800-524-6779 ext. 104 704-970-3304 Direct Line shar...@celligent.net ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: RE: [Histonet] CD31 on rat brain
hi, i am trying to stain the rat CD31 the cat: 550274 is the anti-mouse CD31 have anyone tried this one: http://www.bdbiosciences.com/external_files/pm/doc/tds/rat/live/web_enabled/22711D_555025.pdf test it on parafin sections? 2009-03-12 TF 发件人： Chiriboga, Luis 发送时间： 2009-03-07 00:16:34 收件人： ti...@foxmail.com; Colleen Forster 抄送： histonet; ΕΛΕΝΗ ΚΟΝΣΟΛΑΚΗ 主题： RE: [Histonet] CD31 on rat brain For mouse (specifically) have used CD31 from BD catalog number 550274. Its a rat ant-mouse (Clone MEC13.3). can supply more info if need. The problem I have found is finding good control material for this marker. especially since my only source tends to be the investigators that provide me the samples to begin with. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of TF Sent: Friday, March 06, 2009 10:23 AM To: Colleen Forster Cc: histonet; ΕΛΕΝΗ ΚΟΝΣΟΛΑΚΗ Subject: [Histonet] CD31 on rat brain Just wonder anyone has a suggestion of antibody that works on rat brain? Frozen section and parafin sections? It would be great if this also works on mice. 2009-03-06 TF 发件人： Colleen Forster 发送时间： 2009-03-06 00:57:29 收件人： tifei 抄送： iskaliora; histonet; ΕΛΕΝΗ ΚΟΝΣΟΛΑΚΗ 主题： Re: [Histonet] staining brain vessels You have to have a specific CD31 to work on mouse brain. Very few of them do. Colleen Forster TF wrote: hi, CD31 works great in my section alpha-SMA also works another way is to perfuse the brain with BSA-rhodamine. you will get the fluorescence without the need of staining. 2009-03-05 TF 发件人： iskaliora 发送时间： 2009-03-05 18:49:11 收件人： histonet 抄送： ΕΛΕΝΗ ΚΟΝΣΟΛΑΚΗ 主题： [Histonet] staining brain vessels I was wondering if anybody might have an idea with the following problem we are experiencing: we want to stain for blood vessels in sections of mouse brain. Our experimental tissues have been fixed overnight in 4% paraformaldehyde and have been sitting in PBS since. We have tried staining with antibodies against desmin, SMA, and collagen but we get NO specific signal. We recently tried a non-fixed mouse brain and got desmin to work immediately. The problem is that we need to use the fixed brains because they are our experimental model and it would take too long (2 years to be exact) to generate the same samples. If anybody has come across such a problem before, or has a specific protocol for vessels that works on PFA fixed brain, we would appreciate the suggestions! thanks in advance! Irini - Irini Skaliora, PhD Investigator C᾽ (Assistant Professor) Developmental Biology Division Biomedical Research Foundation of the Academy of Athens (BRFAA) Soranou Efessiou 4 Athens 11527 tel. +30-210-6597203 (office) tel. +30-210-6597482 (lab) fax. +30-210-6597545 email: iskali...@bioacademy.gr ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain information that is proprietary, confidential, and exempt from disclosure under applicable law. Any unauthorized review, use, disclosure, or distribution is prohibited. If you have received this email in error please notify the sender by return email and delete the original message. Please note, the recipient should check this email and any attachments for the presence of viruses. The organization accepts no liability for any damage caused by any virus transmitted by this email. = ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] slide disposal
Thank you Joyce - The SlydEater pulverizes the glass slide and obliterates the label affixed to the slide. Units are available for both short term and long term rentals, as well as Least to Own. If interested, visit our website at: http://www.SlydEater.com or by calling me directly at 800-233-3721. Mark J. Griffith E-mail: mailto:m...@vyleater.com S G Enterprises, Inc. N115 W19000 Edison Drive Germantown, WI 53022 USA Tel: 262/251-8300 US/Canada toll-free: 800/233-3721 Fax: 262/251-1616 From: Joyce Cline jcl...@wchsys.org To: Histonet histonet@lists.utsouthwestern.edu Date: Wed, 11 Mar 2009 12:51:14 -0400 We use the same company that takes away our alcohol waste. They pulverize the slides for us. There is a company in WI that makes a Slydeater that you can rent for one month to pulverize your slides. I am looking into that aspect. Joyce Cline, H.T. (ASCP) Technical Specialist Hagerstown Medical Lab. 301-665-4980 fax 301-665-4941 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sharon Campbell Sent: Wednesday, March 11, 2009 4:57 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] slide disposal Hi everyone! Thank you for all the great responses to my last question about metal vs. plastic molds. I have another question being debated however, How to dispose of slides once the required time is up (10 years for us). We have put the slide in sharps containers and then into biohazard, but are they really bioharzard, the slides only contain the patients accession number - no personal ID on the slides? How do you guys dispose of your slides when space and money are an issue? Thank you Sharon Campbell, HTL(ASCP)CM, BSBM Histology Supervisor Celligent Diagnostics, LLC Formerly Pathology Associates Services 101 East W.T. Harris Blvd, Suite 1212 Charlotte, NC 28262 800-524-6779 ext. 104 704-970-3304 Direct Line shar...@celligent.net ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Bad sections
Hi Histo netters, I am am my wit's end. I know how to section but my sections are compressing like crazy. I've altered my processing protocol thinking that I was overprocessing my samples. I've tried different knife angles, different brands of razor blade knives. It's either me or the microtome. Info- I process animal tissues with between 30-60 minute steps. Vacuum paraffins for 30 minutes. Section with Reichert-Jung 2030 set at 4-ish degrees (a little below the middle line on the knife holder). Soak blocks on Downey ice blocks, water bath at 44 degrees. Suggestions? Waa, Paula Paula Sicurello VA Medical Center San Diego Veterans Medical Research Foundation (VMRF) Core Research Imaging Center 3350 La Jolla Village Dr., MC151 San Diego, CA 92161 858-552-8585 x2397 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] detergent
Hi Rene, To clarify your post: Do you mean liquid dish soap (like palmolive) is to be used? When you say not dishwasher detergent I think of Automatic Dishwasher detergent such as Cascade. Or no dish soap at all and I should use liquid laundry detergent? Thanks. Leslie From: Rene J Buesa rjbu...@yahoo.com Subject: Re: [Histonet] problem eyes To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu, Perry, Margaret margaret.pe...@sdstate.edu Date: Wednesday, March 4, 2009, 9:14 PM Add a few drops (4-5) of liquid detergent, but not dishwasher detergent (because it will dissolve the paraffin). reni J. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Bad sections
What animal tissues are you using? If rodent, may need less time on the steps - like 10-20 min. --On Wednesday, March 11, 2009 11:29 AM -0700 Va Paula Sicurello vapat...@yahoo.com wrote: Hi Histo netters, I am am my wit's end. I know how to section but my sections are compressing like crazy. I've altered my processing protocol thinking that I was overprocessing my samples. I've tried different knife angles, different brands of razor blade knives. It's either me or the microtome. Info- I process animal tissues with between 30-60 minute steps. Vacuum paraffins for 30 minutes. Section with Reichert-Jung 2030 set at 4-ish degrees (a little below the middle line on the knife holder). Soak blocks on Downey ice blocks, water bath at 44 degrees. Suggestions? Waa, Paula Paula Sicurello VA Medical Center San Diego Veterans Medical Research Foundation (VMRF) Core Research Imaging Center 3350 La Jolla Village Dr., MC151 San Diego, CA 92161 858-552-8585 x2397 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Merced M Leiker Research Technician II 354 Biomedical Research Building School of Medicine and Biomedical Sciences State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 Ph: (716) 829-6033 Fx: (716) 829-2725 No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] IHC chromogen loss, HELP!
Posting for a friend.. We have hit a bump in the road with our IHC test and thought maybe you might have some input as we seem to be stuck. We stain (typically for virus) frozen bovine tissues using Biocare's Mach-3 AP polymer kit and develop with Vector Red, counterstain, and dehydrate and mount with Histoclear/VectaMount by hand or xylene/Surgipath mountant on the autocoverslipper. We have been noticing a major loss of stain after development recently. The controls seem to remain stained, it's the more diffuse and minimally stained sections that we have seen stain disappear between development and coverslipping. I did a test with Impress-DAB and Impress-Bajoran Purple to compare and we thought we had solved the problem as being related to the surgipath mountant. However I just did a run with cell markers coverslipping with the Histoclear/Vectamount and there seems to be a loss of stain again. Vector suggested trying 200 mM TBS for wash/diluent/stop instead of 100 mM. This is a recent problem as we have been using this protocol successfully for a few years now. Any suggestions would be greatly appreciated! Replies directly to meghan.tuc...@ars.usda.gov would be appreciated ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] validation documentation for processors
Rene, I agree with your process as to validation, however, could you help me understand where does the CAP state (what check off list item) concerning validation requirements when changing out processors or processing technologies. My staff is currently performing the validation process with a large microwave processor. If CAP does have a recommendation I would appreciate seeing it. I have a (1) out of (12) pathologists that (I think is going overboard) who would like my Pathology Manager to perform over 100 tissue samples for validation process to include IHC/FISH/special staining as we make the switch from conventional to microwave processing. Thanks! Michael Michael R. LaFriniere Executive Director Cytology Services of Maryland (CSM) 301-206-2555 ext 27 301-206-2595 fax michael.lafrini...@csmlab.com On 3/10/2009 at 5:23 PM, Rene J Buesa rjbu...@yahoo.com wrote: As all validations, you will have to process at least 25 pieces (CAP requires from 25 to 100) of tissue using your old and your new processor simultaneously. Make slides and later prepare HE and some HC and IHC procedures using both sets of slides and give them to as many pathologists as you can so they can select, without knowing which section comes from which processor, with only two options: either one section is better than the other, or both are equivalent for diagnostic purposes. Later your data should be analyzed statistically with the chi-square test or you could obviate the test if almost (more than 90%) of all the pairs are qualified as equivalent. René J. --- On Tue, 3/10/09, Joseph Fear fe...@bronsonhg.org wrote: From: Joseph Fear fe...@bronsonhg.org Subject: [Histonet] validation documentation for processors To: histonet@lists.utsouthwestern.edu Date: Tuesday, March 10, 2009, 4:45 PM A question came up in our lab about validation documentation for our new processor. Does anyone have any creative feedback on how your lab documents validation of machines like processors and stainers? See below Joseph Fear 03/01/09 I'll post the question at histonet and see what i can find out about how other histo labs document validation of thier machines. I think with the peloris we ran test cases and Dr.Pearson checked the HE slides, but you're right that we don't have documentation of what cases were run and with JP's signiture for approval of validation. I can work on a general 'machine validation form' in excel and get back with you. -Joseph Virginia Rupert 02/20/09 3:44 PM In your searches, or past experience, do you know or can you find out how new instruments are validated? I don't think we have adequate documentation for the Peloris, with test cases, etc. But I also haven't found a document to use as a template for our purposes. Any ideas? Thanks ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] HTLV-1 IHC
Is anyone performing IHC on FFPE human tissue for HTLV-1? Many Thanks, Jim ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] paraffin oil-clearing agent
Yes!! Paraffin oil = mineral oil. Under separate cover I am sending you an article I wrote on the subject. René J. --- On Wed, 3/11/09, anjan kumar drvet_an...@hotmail.com wrote: From: anjan kumar drvet_an...@hotmail.com Subject: [Histonet] paraffin oil-clearing agent To: triple immunohistochem histonet@lists.utsouthwestern.edu Date: Wednesday, March 11, 2009, 1:15 PM hi everyone, last week i had aquestion regarding xylene substitutes and Vector Blue compatibility. i came across an article saying use of paraffin oil as substitute for xylene... can paraffin oil be used as clearing agent ? _ How fun is this? IMing with Windows Live Messenger just got better. http://www.microsoft.com/india/windows/windowslive/messenger.aspx___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Compromised specimens
As long as everything is solved and there is no doubt about whose specimen it is, I don't see any benefit in letting people know that is was initially compromised. René J. --- On Wed, 3/11/09, Bell, Lynne lynne.b...@cvmc.org wrote: From: Bell, Lynne lynne.b...@cvmc.org Subject: [Histonet] Compromised specimens To: Histonet (histonet@lists.utsouthwestern.edu) histonet@lists.utsouthwestern.edu Date: Wednesday, March 11, 2009, 1:17 PM When our histology lab receives a compromised specimen (not labeled with patient's name, wrong DOB, no label whatsoever, etc) we have the offending party correct the error and fill out an accountability form accepting full responsibility for the identification of the specimen. We then scan the accountability form into the patient's medical record and include the information in the specimen comments section of the pathology record. This information does not print on the official pathology report. My question - should the pathologist include in his/her report the compromised specimen information? Presently, on our clinical lab reports and cytology reports it is included - simply saying Compromised Specimen. Our pathologists historically have not wanted to include this in the official pathology report and I feel that it should be in there - sort of a cover your butt addendum. I'm curious what other hospitals do!! Thanks in advance, Lynne A. Bell, HT (ASCP) Lead Histologist Central Vermont Medical Center 130 Fisher Road Barre, VT 05641 802-371-4923 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Fw: Re: detergent
Leslie: If you use Cascade, Palmolive or any other dishwasher liquid soap the paraffin will be dissolved and you will ruin your sections. I am referring to laundry liquid soap or mild hand washing liquid soap. René J. --- On Wed, 3/11/09, Leslie Chen lc...@mednet.ucla.edu wrote: From: Leslie Chen lc...@mednet.ucla.edu Subject: detergent To: rjbu...@yahoo.com Date: Wednesday, March 11, 2009, 2:52 PM Hi Rene, To clarify your post: Do you mean liquid dish soap (like palmolive) is to be used? When you say not dishwasher detergent I think of Automatic Dishwasher detergent such as Cascade. Or no dish soap at all and I should use liquid laundry detergent? Thanks. Leslie From: Rene J Buesa rjbu...@yahoo.com Subject: Re: [Histonet] problem eyes To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu, Perry, Margaret margaret.pe...@sdstate.edu Date: Wednesday, March 4, 2009, 9:14 PM Add a few drops (4-5) of liquid detergent, but not dishwasher detergent (because it will dissolve the paraffin). reni J. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Bad sections
Compressed sections usually are the result of: 1- blunt knife 2- too soft paraffin for the selected thickness 3- too warm paraffin (block not cold enough) 4- blade too vertical (not enough clearing angle) 5- cutting too fast, specially with thin sections 6- too warm blade 7- microtome set screws loose or defective. René J. --- On Wed, 3/11/09, Va Paula Sicurello vapat...@yahoo.com wrote: From: Va Paula Sicurello vapat...@yahoo.com Subject: [Histonet] Bad sections To: HistoNet histonet@lists.utsouthwestern.edu Date: Wednesday, March 11, 2009, 2:29 PM Hi Histo netters, I am am my wit's end. I know how to section but my sections are compressing like crazy. I've altered my processing protocol thinking that I was overprocessing my samples. I've tried different knife angles, different brands of razor blade knives. It's either me or the microtome. Info- I process animal tissues with between 30-60 minute steps. Vacuum paraffins for 30 minutes. Section with Reichert-Jung 2030 set at 4-ish degrees (a little below the middle line on the knife holder). Soak blocks on Downey ice blocks, water bath at 44 degrees. Suggestions? Waa, Paula Paula Sicurello VA Medical Center San Diego Veterans Medical Research Foundation (VMRF) Core Research Imaging Center 3350 La Jolla Village Dr., MC151 San Diego, CA 92161 858-552-8585 x2397 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Bad sections
Did you change paraffins lately? Softer paraffins, ie., lower melting point, have more compressibility. Peggy A. Wenk, HTL(ASCP)SLS Beaumont Hospital Royal Oak, MI 48073 - Original Message - From: Va Paula Sicurello vapat...@yahoo.com To: HistoNet histonet@lists.utsouthwestern.edu Sent: Wednesday, March 11, 2009 11:29 AM Subject: [Histonet] Bad sections Hi Histo netters, I am am my wit's end. I know how to section but my sections are compressing like crazy. I've altered my processing protocol thinking that I was overprocessing my samples. I've tried different knife angles, different brands of razor blade knives. It's either me or the microtome. Info- I process animal tissues with between 30-60 minute steps. Vacuum paraffins for 30 minutes. Section with Reichert-Jung 2030 set at 4-ish degrees (a little below the middle line on the knife holder). Soak blocks on Downey ice blocks, water bath at 44 degrees. Suggestions? Waa, Paula Paula Sicurello VA Medical Center San Diego Veterans Medical Research Foundation (VMRF) Core Research Imaging Center 3350 La Jolla Village Dr., MC151 San Diego, CA 92161 858-552-8585 x2397 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
