RE: [Histonet] marine animals and insects/ arthrops histotechnology
Hi Ana, Yes, I work for an aquaculture veterinary pathology lab and have experience with some of these, but particularly fish. Please feel free to contact me for more information, I'll help if I can. Debbie Faichney Histopathology Institute of aquaculture University of Stirling Stirling Scotland UK -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ana Resendes Sent: 02 April 2009 19:40 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] marine animals and insects/ arthrops histotechnology Anyone out there expertize or with a good background or working experience in marine (fish, algae, shell moluscles crustaceans) and insects or arthropds histotechnology ? Thank you. Ana -- Ana Resendes DVM, MSc, PhD Veterinary Pathologist Centro de Investigação de Recursos Naturais (CIRN) Secção de Anatomia e Taxonomia Zoológicas, Departamento de Biologia, Universidade dos Açores. Rua da Mãe de Deus, 58 - Apartado 1422 P - 9501-801 Ponta Delgada (Açores) Portugal Tel. (+351) 296 650 000 ext. 1109 Fax (+351) 296 650 100 http://www.uac.pt/~pherg/ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Academic Excellence at the Heart of Scotland. The University of Stirling is a charity registered in Scotland, number SC 011159. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Histonet Digest, Vol 65, Issue 7
Hello Histonetters, I understand most of the professionals on this website are histology professionals, however, I thought I would give it a try since I have not found a cytology listserv yet. Does anyone know how many slides per day a cytotechnologist would screen within a private lab setting (On average)? Alyssa Peterson Allied Search Partners O: 770.621.2639 ext. 4 F: 770.621.2640 Hi Alyssa - Get ready...here is the dirt CAP follows CLIA-88, and both are quite clear on limits for Cytotech screening. They are no more than 100 slides in 24 hours and include gyn and non-gyn including new routine slides, 10% rescreen slides, and 5 year look back negative slides the maximum workload of 100 slides can be completed in no less than an 8-hour workday. This workload can also be expressed as slides per hour and is 12.5 slides/hour. These total limits apply regardless of the number of laboratories in which an individual works on a given day. Wait! it gets better For primary screening of gyn liquid based preps (read as Thin Prep) each slide must be counted as a single slide for the purpose of workload recording. For primary screening of non-gyn liquid based preps (read as Thin Prep) each slide may be counted as one-half slide for the purpose of workload recording, provided that the cells are dispersed over one-half or less of the total available slide area. Wait! there's still more... Workload calculations may vary with the use of automated screening instruments. Laboratories should follow manufacturers instructions for workload calculations and must assure that CLIAA-88 requirements are fulfilled. and just when you thought it was safe The laboratory director must establish the maximum workload (based on the capability/documented performance evaluation) for each individual examining slides and the limit must be reassessed at least every 6 months. The regulations go on to explain how the reassessment is to be done...blah, blah, blah Though my background and education are in Histology, I've served as technical supervisor over cytology departments for over 14 years, and the above listed %#@! is why Cytology can drive you up the wall. Our cytotech's, when we had them, screened no more than 80 slides/day, when all they did was screen. No pulling slides, no running up to do an FNAjust sitting non-stop and screening slides. UGH! Sorry Histo folk - but some of you are probably in the same position as I am, so please feel free to chime in. Terri Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital and Medical Center 1648 Huntingdon Pike Meadowbrook, PA 19046 (215) 938-3676 phone (215) 938-3689 fax - CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Tissue-Tek II Manual
Updated request with model number. Does anyone have a copy of the Tissue-Tek II manual that they would be willing to share? (PDF preferred, hard-copy graciously accepted) One for a model 4553 Cryostat (apologies for not including that in the original post) Thanks (in advance), Bernie Ball Duke University ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] RE: Histonet Digest, Vol 65, Issue 7
Terri: Any private setting (including large reference labs) is ruled by the same standards as any other one. The screener threshold (when you need to hire an additional one) is 7,000 slides/year. Under separate cover I am sending you an article I wrote about the Cytology work within the Histology lab. René J. --- On Fri, 4/3/09, Terri Braud tbr...@holyredeemer.com wrote: From: Terri Braud tbr...@holyredeemer.com Subject: [Histonet] RE: Histonet Digest, Vol 65, Issue 7 To: histonet@lists.utsouthwestern.edu Date: Friday, April 3, 2009, 8:42 AM Hello Histonetters, I understand most of the professionals on this website are histology professionals, however, I thought I would give it a try since I have not found a cytology listserv yet. Does anyone know how many slides per day a cytotechnologist would screen within a private lab setting (On average)? Alyssa Peterson Allied Search Partners O: 770.621.2639 ext. 4 F: 770.621.2640 Hi Alyssa - Get ready...here is the dirt CAP follows CLIA-88, and both are quite clear on limits for Cytotech screening. They are no more than 100 slides in 24 hours and include gyn and non-gyn including new routine slides, 10% rescreen slides, and 5 year look back negative slides the maximum workload of 100 slides can be completed in no less than an 8-hour workday. This workload can also be expressed as slides per hour and is 12.5 slides/hour. These total limits apply regardless of the number of laboratories in which an individual works on a given day. Wait! it gets better For primary screening of gyn liquid based preps (read as Thin Prep) each slide must be counted as a single slide for the purpose of workload recording. For primary screening of non-gyn liquid based preps (read as Thin Prep) each slide may be counted as one-half slide for the purpose of workload recording, provided that the cells are dispersed over one-half or less of the total available slide area. Wait! there's still more... Workload calculations may vary with the use of automated screening instruments. Laboratories should follow manufacturers instructions for workload calculations and must assure that CLIAA-88 requirements are fulfilled. and just when you thought it was safe The laboratory director must establish the maximum workload (based on the capability/documented performance evaluation) for each individual examining slides and the limit must be reassessed at least every 6 months. The regulations go on to explain how the reassessment is to be done...blah, blah, blah Though my background and education are in Histology, I've served as technical supervisor over cytology departments for over 14 years, and the above listed %#@! is why Cytology can drive you up the wall. Our cytotech's, when we had them, screened no more than 80 slides/day, when all they did was screen. No pulling slides, no running up to do an FNAjust sitting non-stop and screening slides. UGH! Sorry Histo folk - but some of you are probably in the same position as I am, so please feel free to chime in. Terri Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital and Medical Center 1648 Huntingdon Pike Meadowbrook, PA 19046 (215) 938-3676 phone (215) 938-3689 fax - CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] keratinocyte marker
Hi all, Can anyone suggest a evolutionary conserved keratinocyte marker? I'm working with a fish (not zebrafish!) and need an antibody marker that can ID keratinocytes. Thanks -- Tyrone Genade http://tgenade.freeshell.org email: tgen...@freeshell.org tel: +27-84-632-1925 (c) For there is one God, and there is one mediator between God and men, the man Christ Jesus, who gave himself as a ransom for all. 1 Timothy 2:5-6 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] unsubscribe
Frances L. Swain HT(ASCP) A. A. S. Special Procedures Technician Department of Orthopaedic Surgery Center for Orthopaedic Research Barton Research Building 2R28 4301 West Markham Street Little Rock AR 72205 (501) 686-8739 PHONE (501) 686-8987 FAX swainfranc...@uams.edumailto:swainfranc...@uams.edu email Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Cryostat sections mounting with 0.1M PB solution onto slides
After we cut the brain sections on a cryostat (OCT embedded), we brush a drop of 0.1M PB on to the slide before mounting the sections. Do anyone know the side effect of this - for example, the sections will peel off during immunostaining? Another question is about the dry time after mounting and before staining. I know some people asked similar questions before, but they are using fresh tissue for frozen sections. We here perfuse the animal, post-fix the tissue and sink it in sucrose before making cryostat sections. So we may dry the sections in air up to weeks. I would like to ask about the proper heating time (with a heat plate) before dry the sections up in a fumehood. Should we heat the sections at 60 degree for 10 min or 2 hours shortly after mounting? Thanks very much. 2009-04-03 TF ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Protein Block
We recently purchased a Dako Autostainer with the PT Link for our IHC's. We are experiencing more background staining then before when we had the older version of the Dako Autostainer. Does anybody use protein block (serum free) to help minimize background staining? Thank you for your replies. Jason McGough HT(ASCP) Account Representative - Anatomic Pathology Clinical Laboratory of the Black Hills 2805 5th Street Suite 210 Rapid City, SD 57701 605-343-2267 Ext 127 605-718-3779 (Fax) jmcgo...@clinlab.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Strange Circles in IHC slides
The circles could be either from air bubbles present when you mount the slides onto the coverplates, or they could have arisen after exposure to H2O2 as part of the staining procedure. Doing the H2O2 off instrument in a coplin jar and then rinsing and mounting them in the coverplates will help keep that from happening. Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Histo license
I have a license to drive a car, and a certification that allows me to pay each year to ASCP. I feel so credentialed! I think we should all take Saturday and Sunday off. Nick Madary, HT/HTL(ASCP)QIHC Medimmune Histology Laboratory Mgr One Medimmune Way, Lab 2438-Area 4 Gaithersburg, MD 20878 ph 301.398.4745/6360 fx 301.398.9745 To the extent this electronic communication or any of its attachments contain information that is not in the public domain, such information is considered by MedImmune to be confidential and proprietary, and expected to be used only by the individual(s) for whom it is intended. If you have received this electronic communication in error, please reply to the sender advising of the error in transmission and delete the original message and any accompanying documents from your system immediately, without copying, reviewing or otherwise using them for any purpose. Thank you for your cooperation. P Please consider the environment before printing this e-mail To the extent this electronic communication or any of its attachments contain information that is not in the public domain, such information is considered by MedImmune to be confidential and proprietary. This communication is expected to be read and/or used only by the individual(s) for whom it is intended. If you have received this electronic communication in error, please reply to the sender advising of the error in transmission and delete the original message and any accompanying documents from your system immediately, without copying, reviewing or otherwise using them for any purpose. Thank you for your cooperation. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Tonsil Control Blocks Needed
Fellow Histonetters, I am having an extremely hard time obtaining tonsil control tissue and was wondering if anyone would have some they could share. I would be glad to exchange some rhabdomyosarcoma tissue blocks in exchange. Please let me know if you can help. Thanks, Charlene Henry HT (ASCP), QIHC Anatomic Pathology Section Head Department of Pathology St. Jude Children's Research Hospital 262 Danny Thomas Place Memphis, TN 38105 901-595-3191 fax 901-595-3100 Email Disclaimer: www.stjude.org/emaildisclaimer ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Tonsil Control Blocks Needed
Contact me off line and I'd be happy to see what we can do to accommodate you at jrobert...@pathologysciences.com Thank you. Jodie Robertson, HT (ASCP) QIHC Pathology Sciences Medical Group Histology Day Supervisor 183 E. 8th Ave. Chico, CA 95926 530-891-6244 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Henry, Charlene Sent: Friday, April 03, 2009 10:35 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Tonsil Control Blocks Needed Fellow Histonetters, I am having an extremely hard time obtaining tonsil control tissue and was wondering if anyone would have some they could share. I would be glad to exchange some rhabdomyosarcoma tissue blocks in exchange. Please let me know if you can help. Thanks, Charlene Henry HT (ASCP), QIHC Anatomic Pathology Section Head Department of Pathology St. Jude Children's Research Hospital 262 Danny Thomas Place Memphis, TN 38105 901-595-3191 fax 901-595-3100 Email Disclaimer: www.stjude.org/emaildisclaimer ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Removing PMMA blocks from glass vials
Someone gave me some small PMMA blocks polymerized inside glass scintillation vials. I always use plastic vials. Obviously the vials have to be broken to get the blocks out. Does anyone have some advice on the best way to do this? The guy who brought them said he thought I could use liquid nitrogen, but had no specifics. Any help will be appreciated. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Removing PMMA blocks from glass vials
Hi Paul, I did so many of those PMMA blocks cracking. Take a under pad, line your blocks not too many and cover with another under pad and start (literally) hammering them one by one with hammer gently I am not kidding. Make sure you wear safety glasses and double gloves to protect your hands. When the blocks are cracked remove them and rinse with tap water and small glass pieces will go into sharp container. I know seems very complicated but it is not. If you have more questions regarding this please fell free to contact me. Good Luck Shakun -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Monfils, Paul Sent: Friday, April 03, 2009 11:00 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Removing PMMA blocks from glass vials Someone gave me some small PMMA blocks polymerized inside glass scintillation vials. I always use plastic vials. Obviously the vials have to be broken to get the blocks out. Does anyone have some advice on the best way to do this? The guy who brought them said he thought I could use liquid nitrogen, but had no specifics. Any help will be appreciated. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Removing PMMA blocks from glass vials
I agree. It is a little messy and can be dangerous if you are not paying enough attention. LN just gets the glass cold and cracks it so you don't need a hammer. If you leave it in the LN too long the block may crack with the vial. Pam Marcum UPENN Vet School New Bolton Center - Original Message - From: Shakun Aswani shakun.asw...@acologix.com To: Paul Monfils pmonf...@lifespan.org, histonet@lists.utsouthwestern.edu Sent: Friday, April 3, 2009 2:23:30 PM GMT -05:00 US/Canada Eastern Subject: RE: [Histonet] Removing PMMA blocks from glass vials Hi Paul, I did so many of those PMMA blocks cracking. Take a under pad, line your blocks not too many and cover with another under pad and start (literally) hammering them one by one with hammer gently I am not kidding. Make sure you wear safety glasses and double gloves to protect your hands. When the blocks are cracked remove them and rinse with tap water and small glass pieces will go into sharp container. I know seems very complicated but it is not. If you have more questions regarding this please fell free to contact me. Good Luck Shakun -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Monfils, Paul Sent: Friday, April 03, 2009 11:00 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Removing PMMA blocks from glass vials Someone gave me some small PMMA blocks polymerized inside glass scintillation vials. I always use plastic vials. Obviously the vials have to be broken to get the blocks out. Does anyone have some advice on the best way to do this? The guy who brought them said he thought I could use liquid nitrogen, but had no specifics. Any help will be appreciated. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Entamoeba Trophozoites
I need help! Just received a needle biopsy of liver submitted in normal saline. They did all the routine cultures/ fungal, and are requesting a wet mount on this tissue for Entamoeba Trophozoites. I have contacted all of my resources in and out of state, and they cannot help me. HELP!! thanks, Lynette Lynette Pavelich, HT(ASCP) Histology Supervisor Hurley Medical Center One Hurley Plaza Flint, MI 48503 ph: 810-257-9948 fax: 810-762-7082 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Removing PMMA blocks from glass vials
Paul, Put in LN2 (or leave in -80 freezer for about 1 hour). Using the appropriate PPE, wrap in towel, hit with Hammer (gently...), welcome to use this SOP. Bob, Robert Schoonhoven From: Monfils, Paul pmonf...@lifespan.org To: histonet@lists.utsouthwestern.edu Sent: Friday, April 3, 2009 2:00:20 PM Subject: [Histonet] Removing PMMA blocks from glass vials Someone gave me some small PMMA blocks polymerized inside glass scintillation vials. I always use plastic vials. Obviously the vials have to be broken to get the blocks out. Does anyone have some advice on the best way to do this? The guy who brought them said he thought I could use liquid nitrogen, but had no specifics. Any help will be appreciated. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Removing PMMA blocks from glass vials
I would not use liquid nitrogen. The safest way to do this is to put the blocks in a refrigerator freezer for a couple of hours. Remove quickly, remove caps, wrap in paper towels and hit with a hammer. Be sure to wear protective clothing, eye covers and gloves. When the glass has broken into the towel put the towel in a sharps container, rinse the block and procees with the sectioning. Frances L. Swain HT(ASCP) A. A. S. Special Procedures Technician Department of Orthopaedic Surgery Center for Orthopaedic Research Barton Research Building 2R28 4301 West Markham Street Little Rock AR 72205 (501) 686-8739 PHONE (501) 686-8987 FAX swainfranc...@uams.edu email -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Monfils, Paul Sent: Friday, April 03, 2009 1:00 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Removing PMMA blocks from glass vials Someone gave me some small PMMA blocks polymerized inside glass scintillation vials. I always use plastic vials. Obviously the vials have to be broken to get the blocks out. Does anyone have some advice on the best way to do this? The guy who brought them said he thought I could use liquid nitrogen, but had no specifics. Any help will be appreciated. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Problems with mouse brains
Folks, I need some input on a problem we're seeing with some mouse brain samples. The samples are from new born mice P0 to P14 which have been fixed in 10% NBF (Fisher brand and well within expiration date) for 72 hours. They are then transferred to 70% etoh and processed on an 8 hour process. After the tissue is embedded, we are taking sagital sections, drying them overnight, and then baking overnight at 60C. They are then stained with HE. This has been working great but now all of a sudden we have very light HE staining, nuclear bubbling, and extensive tissue cracking. I also have noticed some formalin pigment. My first thought is that there was difference in formalin or time in formalin but that is not the case. I also wondered if the tissue might be exposed to excessive heat. We checked all of the temperatures in our processor, embedding center, and water baths - all were within tolerance. Any ideas what might cause all three artifacts? Could it be from inadequate paraffin infiltration? Any help would be greatly appreciated! Thanks, Julie Julie Randolph-Habecker, Ph.D. Staff Scientist - Director Experimental Histopathology Shared Resource Fred Hutchinson Cancer Research Center 1100 Fairview Ave, N. DE-360 (Please note new location) Seattle WA 98109-1024 206-667-6119 jhabe...@fhcrc.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Technovit 7200 (isobornylmethacrylate) staining
This question is specifically for Linda Jenkins or anyone who has worked with Technovit 7200, I am trying to do an HE stain on ~70 um thick ground sections on plastic slides. I have followed the Donath method all the way through processing, but the sections do not pick up stains the way the booklet says they should. I am even having trouble following GMA staining procedures that I have found, in fact am afraid to try too many because the acrylic forms tiny cracks that interfere with microscopy. I have researched the histonet archives, but haven't found anything other than a couple of similar inquiries. I would very much appreciate any advice from people who have worked with this specific formulation. Happy Friday! Lori [CONFIDENTIALITY AND PRIVACY NOTICE] Information transmitted by this email is proprietary to Medtronic and is intended for use only by the individual or entity to which it is addressed, and may contain information that is private, privileged, confidential or exempt from disclosure under applicable law. If you are not the intended recipient or it appears that this mail has been forwarded to you without proper authority, you are notified that any use or dissemination of this information in any manner is strictly prohibited. In such cases, please delete this mail from your records. To view this notice in other languages you can either select the following link or manually copy and paste the link into the address bar of a web browser: http://emaildisclaimer.medtronic.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Technovit 7200 (isobornylmethacrylate) staining
To my knowledge it is near impossible to obtain an adequate HE stain on thick resin sections. If this is not true, I would be interested in the same information. Jack On Apr 3, 2009, at 6:12 PM, Garcia, Lori, Sr. Scientist lori.gar...@medtronic.com wrote: This question is specifically for Linda Jenkins or anyone who has worked with Technovit 7200, I am trying to do an HE stain on ~70 um thick ground sections on plastic slides. I have followed the Donath method all the way through processing, but the sections do not pick up stains the way the booklet says they should. I am even having trouble following GMA staining procedures that I have found, in fact am afraid to try too many because the acrylic forms tiny cracks that interfere with microscopy. I have researched the histonet archives, but haven't found anything other than a couple of similar inquiries. I would very much appreciate any advice from people who have worked with this specific formulation. Happy Friday! Lori [CONFIDENTIALITY AND PRIVACY NOTICE] Information transmitted by this email is proprietary to Medtronic and is intended for use only by the individual or entity to which it is addressed, and may contain information that is private, privileged, confidential or exempt from disclosure under applicable law. If you are not the intended recipient or it appears that this mail has been forwarded to you without proper authority, you are notified that any use or dissemination of this information in any manner is strictly prohibited. In such cases, please delete this mail from your records. To view this notice in other languages you can either select the following link or manually copy and paste the link into the address bar of a web browser: http://emaildisclaimer.medtronic.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Entamoeba Trophozoites
Anyone else think this Rene fella comes across as a real know-it-all? From: Rene J Buesa rjbu...@yahoo.com To: histonet@lists.utsouthwestern.edu; histonet-boun...@lists.utsouthwestern.edu; Lynette Pavelich lpave...@hurleymc.com Sent: Friday, April 3, 2009 4:12:15 PM Subject: [Histonet] Entamoeba Trophozoites Lynette: Since it was received in saline and the tissue has its normal consistency I would prepare a squash (squash the tissue between 2 glass slides not between coverslips) with a rotary movement so you have 2 large areas of squashed tissue. After that, air dry, fix with methanol, air dry again and stain with Giemsa. That is what I would do. Try it if you do not have a better option. René J. --- On Fri, 4/3/09, Lynette Pavelich lpave...@hurleymc.com wrote: From: Lynette Pavelich lpave...@hurleymc.com Subject: [Histonet] Entamoeba Trophozoites To: histonet@lists.utsouthwestern.edu, histonet-boun...@lists.utsouthwestern.edu Date: Friday, April 3, 2009, 3:09 PM I need help! Just received a needle biopsy of liver submitted in normal saline. They did all the routine cultures/ fungal, and are requesting a wet mount on this tissue for Entamoeba Trophozoites. I have contacted all of my resources in and out of state, and they cannot help me. HELP!! thanks, Lynette Lynette Pavelich, HT(ASCP) Histology Supervisor Hurley Medical Center One Hurley Plaza Flint, MI 48503 ph: 810-257-9948 fax: 810-762-7082 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
