[Histonet] To all spam gourmets
Please, stop spamming this list. Comment if you know something useful. Otherwise keep it. I don't mind what you had for breakfast in June 1st last year or your opinion on my clothes' colour. Believe it or not, there IS a real life ( link: http://en.wikipedia.org/wiki/Real_life_(reality) ), and maybe you should check it out - it has way more to offer than waiting for responses to mock about. Thanks. Valentin PS: What about a moderated board? Every inappropriate comment on HISTO TOPIC could be moved or even deleted, except in the spamming forum which can be found on nearly every board on the net. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] I recommend NOT opening that Attachment:
Fellow histology and related fields professionals, I'm not opening this. From a call name of u know (u_deserve_2_k...@yahoo.com). The last time I opened something like this, from a name like this, I ended up owing money for Viagra treatments for Panda bears or something like that. It may be real, but it probably is spam. There...has been...a lot...of it...recently! By the way, there is no such thing as a Virus-proof LINK! Not sure? Just Google it. If you need Spyware or Antivirus software, check your IT department. Also, CNET has free downloads of AdAware, or AVG antivirus 8. And now for a joke (I think we all need it); A physician is doing his rounds and a nurse stops him in the hallway, Doctor, can you sign these reports? Fine he says and proceeds to pull out a rectal thermometer from his coat pocket. He looks at it in disbelief and exclaims, Darn it! Some a$$ has my pen! Respectfully, Hugh Luk, HTL (ASCP) Cancer Research center of Hawaii Pathology shared resources lab manager KP- Hawaii Date: Wed, 8 Apr 2009 21:41:35 -0700 From: u_deserve_2_k...@yahoo.com To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Spyware from an Attachment: Dear Listers, If you ever opened any strange attachments from this list, you should really consider scanning your Hard Disk for spyware. There is a great free anti-spyware program we use, which you can get at http://DELETEDthat is a virus-proof link, by the way. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _ Quick access to your favorite MSN content and Windows Live with Internet Explorer 8. http://ie8.msn.com/microsoft/internet-explorer-8/en-us/ie8.aspx?ocid=B037MSN55C0701A___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] TMA for cell pellets?
Thank you to everyone who responded!! I was given the cell pellets already in the matrix so I only had to process!! It was easier than the mouse tissues!! Since we do so few 20-40 at a time I do everything by hand...no automation in our lab!! -Original Message- From: Bernie Taupin [mailto:bernietau...@ymail.com] Sent: Thu 4/9/2009 1:50 AM To: Thom Jensen; Hartson, Louise; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] TMA for cell pellets? Recently, someone mentioned putting a library of protocols online, perhaps in the HistoNet archives. I'd like to second that emotion if its possible to make happen... What a great resource that would be! From: Thom Jensen tissuear...@hotmail.com To: louise_hart...@urmc.rochester.edu; histonet@lists.utsouthwestern.edu Sent: Thursday, April 9, 2009 1:27:18 AM Subject: [Histonet] TMA for cell pellets? Have you tried to embed your cell pellets into a tissue microarray? Two companies I would recommend: www.arraymold.com www.beecherinstruments.com Two of the best TMA products on the market. cheers, Date: Thu, 2 Apr 2009 12:05:39 -0400 From: louise_hart...@urmc.rochester.edu To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Embedding cell pellets I am looking for a protocol for embedding cell pellets in paraffin. Thanks, Louise Louise Hartson, BA Senior Technical Associate University of Rochester louise_hart...@urmc.rochester.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _ Hotmail® is up to 70% faster. Now good news travels really fast. http://windowslive.com/online/hotmail?ocid=TXT_TAGLM_WL_HM_70faster_032009___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Formula 83 users?
Jacqueline: Formula 83 is a naphthenic hydrocarbon that cannot be used with coverslippers, is recyclable and while some people have been using it for more than 20 years, others find it irritant. René J. --- On Wed, 4/8/09, Jacqueline Farnsworth jacqueline.farnswo...@cls.ab.ca wrote: From: Jacqueline Farnsworth jacqueline.farnswo...@cls.ab.ca Subject: [Histonet] Formula 83 users? To: Histonet Histonet@lists.utsouthwestern.edu Date: Wednesday, April 8, 2009, 12:31 PM HI all. I have searched the Histonet archives and found a lot of very positive reviews regarding Formula 83. I am wondering if anyone has encountered any issues with using Formula 83 on a stainer and then xylene on a tape coverslipper. Are there any miscibility issues? Are you soaking in xylene prior to tape coverslipping required? Any helpful hints/tips/tricks? Thank you in advance. Jacqueline Farnsworth Anatomic Pathology, Tech III Foothills Medical Centre Calgary Laboratory Services Ph: 403-944-1578 Fax: 403-944-4748 P Please consider the environment before printing this email. This message and any attached documents are only for the use of the intended recipient(s), are confidential and may contain privileged information. Any unauthorized review, use, retransmission, or other disclosure is strictly prohibited. If you have received this message in error, please notify the sender immediately, and then delete the original message. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Negative IHC controls
I used to run a negative control per case or per block within any given case when doing IHC, but not for reagents. René J. --- On Wed, 4/8/09, Sheila Haas micropathl...@yahoo.com wrote: From: Sheila Haas micropathl...@yahoo.com Subject: [Histonet] Negative IHC controls To: histonet@lists.utsouthwestern.edu Date: Wednesday, April 8, 2009, 2:57 PM I have a question concerning ANP.22570 on the CAP checklist. Could you all tell me how you are handling negative controls for IHC staining? The question actually states (and I've confirmed with CAP) that we should be running two types of negative controls. One for reagents and one for each antibody in a run. I'd like to know what the practice is. This seems very costly and time consuming. Thanks in advance! Sheila Haas Laboratory Supervisor Micro Path Laboratories ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Formula 83 users?
With all due respect René; I have been using Formula 83 with my coverslipper with no problems at all though have a glass coverslipper not a tape. Linda Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lbla...@digestivespecialists.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Thursday, April 09, 2009 9:56 AM To: Histonet; Jacqueline Farnsworth Subject: Re: [Histonet] Formula 83 users? Jacqueline: Formula 83 is a naphthenic hydrocarbon that cannot be used with coverslippers, is recyclable and while some people have been using it for more than 20 years, others find it irritant. René J. --- On Wed, 4/8/09, Jacqueline Farnsworth jacqueline.farnswo...@cls.ab.ca wrote: From: Jacqueline Farnsworth jacqueline.farnswo...@cls.ab.ca Subject: [Histonet] Formula 83 users? To: Histonet Histonet@lists.utsouthwestern.edu Date: Wednesday, April 8, 2009, 12:31 PM HI all. I have searched the Histonet archives and found a lot of very positive reviews regarding Formula 83. I am wondering if anyone has encountered any issues with using Formula 83 on a stainer and then xylene on a tape coverslipper. Are there any miscibility issues? Are you soaking in xylene prior to tape coverslipping required? Any helpful hints/tips/tricks? Thank you in advance. Jacqueline Farnsworth Anatomic Pathology, Tech III Foothills Medical Centre Calgary Laboratory Services Ph: 403-944-1578 Fax: 403-944-4748 P Please consider the environment before printing this email. This message and any attached documents are only for the use of the intended recipient(s), are confidential and may contain privileged information. Any unauthorized review, use, retransmission, or other disclosure is strictly prohibited. If you have received this message in error, please notify the sender immediately, and then delete the original message. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Spyware from an Attachment:
Dear List: I strongly suggest that you NOT use any links offered by people with no name and no affiliation. Yes, there are good spyware detection programs available at no cost (Adaware for example) but I don't think this is one of them. There are at least 3 potential saboteurs on this list. Geoff u know wrote: Dear Listers, If you ever opened any strange attachments from this list, you should really consider scanning your Hard Disk for spyware. There is a great free anti-spyware program we use, which you can get at http://tinyurl.com/ynupj4 that is a virus-proof link, by the way. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- -- ** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcaul...@umdnj.edu ** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Formula 83-Thank you!
Thank you all for taking time to respond to my inquiry about Formula 83. We received a free sample and will perform some trials and I will post our results on the Histonet asap. Of course, there can always be hidden challenges bringing reagents over the Canadian border, so that in itself can prove interesting, (eh!) Thanks Jacquie Jacqueline Farnsworth Anatomic Pathology, Tech III Foothills Medical Centre Calgary Laboratory Services Ph: 403-944-1578 Fax: 403-944-4748 P Please consider the environment before printing this email. This message and any attached documents are only for the use of the intended recipient(s), are confidential and may contain privileged information. Any unauthorized review, use, retransmission, or other disclosure is strictly prohibited. If you have received this message in error, please notify the sender immediately, and then delete the original message. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] HISTOS 5 processor
Hi, all We get some money recently and want to spend it on the HISTOS 5 proceesor from Smilestone. But we are not sure whether it is worth purchasing. Does anyone here have any experiences or suggestions of it? Thank you, Ann Fu University of Flodrida ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Position Open In Mass
Position Open in Mass Good morning and Happy Spring to everyone. I have a full time permanent position open in Burlington, Ma area. I have included the details below, and don’t forget about our $1000 referral program in place. Please forward this to whomever you feel fit, and if we place one of your referrals in a position than we pay you $1000 as a referral bonus. Thank you histonet and have a great day! *Qualifications: * - Necropsies experience needed - B.S. in Life Sciences or equivalent experience - Animal handling restraint experience with both large and small animals - Dose administration and biological sample collection techniques experience needed - Ability to lift 50 lbs. *Title: * Pharma Technician *Department:* * * Pharma/chem. Services *Status: *Exempt *Shift: *Monday-Friday, Full Time, 8am-5pm, and as required for study performance and weekends. Weekly schedule may be Tuesday-Sat. or Sunday-Thursday. *Benefits: *Healthcare benefits, PTO, Vacation time, 401K, and much more! -- Alyssa Peterson Allied Search Partners O: 770.621.2639 ext. 4 F: 770.621.2640 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] HISTOS 5 processor
Ann, I was able to investigate this unit at Milestone Medical as their US headquarters is located locally here in Kalamazoo, MI. They have an excellent line of products and I recommend them highly. Disclaimer: I have no commercial interest in Milestone Medical. Regards, Bob Schoonhoven, HT/HTL (ASCP) From: FU,DONGTAO f...@ufl.edu To: Histonet Histonet@lists.utsouthwestern.edu Sent: Thursday, April 9, 2009 10:25:56 AM Subject: [Histonet] HISTOS 5 processor Hi, all We get some money recently and want to spend it on the HISTOS 5 proceesor from Smilestone. But we are not sure whether it is worth purchasing. Does anyone here have any experiences or suggestions of it? Thank you, Ann Fu University of Flodrida ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Travelling Histotechs
I have noticed in the past several posts looking for traveling histo techs. Would one of these agencies please contact me off list? 35+ years of experience recently become available. Robert Schoonhoven HT/HTL (ASCP) 269-615-0576 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Regional Sales Manager position- West Coast
This person will need to be based on the West Coast, California preferred. Leica Microsystems is a leading global designer and producer of innovative high-tech precision optics systems for the analysis of microstructures. It is one of the market leaders in each of the fields of Microscopy, Confocal Laser Scanning Microscopy, Imaging Systems, Specimen Preparation and Medical Equipment. Comprising nine manufacturing facilities in seven countries, sales and service companies in 20 countries and an international network of dealers, the company is represented in over 100 countries This person will drive annual sales strategies and goals through management of Sales Representatives and field technical support staff. Develop area sales plans in conjunction with the Biosystems Division Director of Sales to support Leica’s Histology customers. Provide the direction, leadership, coaching and mentoring for direct sales representatives. Advance the professional development, professional conduct, sales effectiveness and efficiency of staff. Regional Sales Management Achieve and exceed Area’s quarterly and annual sales quota, strategic and unit sales mix objectives, profit goal and market share goals. Develop sales plans and strategies to increase market penetration within Area. Participate in annual sales strategy and OPEX planning. Develop annual sales quotas for sales representatives and dealers. Manage and control department expenses at or below budgeted levels. Approve pricing , within authority, for new and demonstration instrument sales. Maintain strategy, support and pricing consistency between sales territories for National/Regional Accounts and National contracts. Maintain relationships with key customers and influencers at major institutions in Area. Sales Representative Management Manage, coach, mentor and support a team of direct sales representatives. Ensure direct sales representative, specialists and ERD’s achieve and exceed individual quarterly and annual sales quota, strategic and unit sales mix objectives. Travel with sales representatives and specialists providing individual coaching and professional development to members of the regional selling team (target 3 days per week or 60% of available time). Complete Field Sales Management Reports and distribute report for each field visit. Establish Standards of Performance for direct reports and set expectations on completion of responsibilities and duties. Ensure direct reports are compliant with all aspects of Leica CRM tool. Ensure sales team adherence to and consistent use of the Leica Value Selling tool. Experience Required BA/BS Degree in Life Science or equivalent 5 plus years experience in sales Preferred MBA/Masters Degree in Life Science 3 plus years sales management experience in selling capital equipment Histology/Cytology/Immunohistochemistry market knowledge Experience in distributor management a plus Leica offers competitive salary, benefits including medical, dental, vision, prescription, long-term care, life insurance, STD, LTD, and 401 (k). Please email your resume to- kris.caldw...@leica-microsystems.com Kris Caldwell Human Resources Recruiter Leica Microsystems, Inc. 2345 Waukegan Road Bannockburn, IL 60015 www.leica-microsystems.com kris.caldw...@leica-microsystems.com 847-405-5432 - phone 847-236-3035 - fax 847-323-6169- cellular __ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email __ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] New lab, need some info
Hi, I called the NSH and they said I might have better luck posting on the histonet but they are sending me all that they have in the way of help in writing manuals for the lab. I am working in a new lab that just opened a few months ago, all derm, building from the ground up. I have all my log sheets generated (all that I can think of anyway) to document temps, maintenance on machines, non pathologist grossing log, block and slide count etc. I have to write all my manuals from scratch. I know that they have to be in NCCLS format but was wondering if anyone could just give me some advise. I have my first inspection, by the state, in August and we are eventually going to be CAP certified. So, I don't want to miss anything. I am the only tech and doing all the grossing, typing my transcription, ordering, basic histology, special stains, IF etc. Any words of wisdom would be greatly appreciated. I just don't want to miss anything and I have OCD and worry myself into a frenzy. I work for a great doctor and want her practice to grow rapidly and I know it will. Thanks for any help you can give. Jodi Jodi Putnam (HT,ASCP) Graves Gilbert Clinic Pathology Department 201 Park Street Bowling Green, KY 42102 (270) 393-2728 (voicemail) (270) 393-2795 Fax : (270) 393-2736 _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Graves-Gilbert Clinic. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] TdT
I'm wondering where labs are purchasing their TdT antibody from. I'm currently using the Dako Autostainer and would be interested in any information on the procedure as well. Thank you! Jean Taylor, HT(ASCP)QIHC IHC Tech Meriter Health Services Madison, WI ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] New lab, need some info
NCCLS is now known as CLSI. They have a standard (document) for the writing of procedure manuals. CAP will require this, so you may want to purchase that document now. Putnam, Jodi putn...@ggclinic.com Sent by: histonet-boun...@lists.utsouthwestern.edu 04/09/2009 09:51 AM To histonet@lists.utsouthwestern.edu cc Subject [Histonet] New lab, need some info Hi, I called the NSH and they said I might have better luck posting on the histonet but they are sending me all that they have in the way of help in writing manuals for the lab. I am working in a new lab that just opened a few months ago, all derm, building from the ground up. I have all my log sheets generated (all that I can think of anyway) to document temps, maintenance on machines, non pathologist grossing log, block and slide count etc. I have to write all my manuals from scratch. I know that they have to be in NCCLS format but was wondering if anyone could just give me some advise. I have my first inspection, by the state, in August and we are eventually going to be CAP certified. So, I don't want to miss anything. I am the only tech and doing all the grossing, typing my transcription, ordering, basic histology, special stains, IF etc. Any words of wisdom would be greatly appreciated. I just don't want to miss anything and I have OCD and worry myself into a frenzy. I work for a great doctor and want her practice to grow rapidly and I know it will. Thanks for any help you can give. Jodi Jodi Putnam (HT,ASCP) Graves Gilbert Clinic Pathology Department 201 Park Street Bowling Green, KY 42102 (270) 393-2728 (voicemail) (270) 393-2795 Fax : (270) 393-2736 _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Graves-Gilbert Clinic. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Edge effect on IHC-P
Hi All, I am having variable staining when performing IHC. I seem to get an edge effect where the edge of the tissue shows increased DAB staining compared to the middle of the tissue. I have tried testing many fixation variables and still haven't found an edge affect culprit. I'm wondering if anyone in the community has experienced and corrected this problem. Thanks for you help! -Sam Perry Dana Farber Cancer Institute Boston MA Research Technician The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Edge effect on IHC-P
Sam, Could you give us a few facts from your protocol? They may help pin down what might be causing your edge effect. How long does your tissue stay on the grossing/trimming table before being put into fixative? What type of fixative are you using? How fresh is it? How long to you fix? What temps to you perform IHC at? How long are your incubations? Etc. Jan Shivers UMN VDL - Original Message - From: Perry, Samuel samuel_pe...@dfci.harvard.edu To: histonet@lists.utsouthwestern.edu Sent: Thursday, April 09, 2009 1:31 PM Subject: [Histonet] Edge effect on IHC-P Hi All, I am having variable staining when performing IHC. I seem to get an edge effect where the edge of the tissue shows increased DAB staining compared to the middle of the tissue. I have tried testing many fixation variables and still haven't found an edge affect culprit. I'm wondering if anyone in the community has experienced and corrected this problem. Thanks for you help! -Sam Perry Dana Farber Cancer Institute Boston MA Research Technician The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] TdT
I used the Tdt from DAKO using their autostainer, diluted 1:10, HIER at pH8 and thymus as control. René J. --- On Thu, 4/9/09, Taylor, Jean jtay...@meriter.com wrote: From: Taylor, Jean jtay...@meriter.com Subject: [Histonet] TdT To: histonet@lists.utsouthwestern.edu Date: Thursday, April 9, 2009, 2:11 PM I'm wondering where labs are purchasing their TdT antibody from. I'm currently using the Dako Autostainer and would be interested in any information on the procedure as well. Thank you! Jean Taylor, HT(ASCP)QIHC IHC Tech Meriter Health Services Madison, WI ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Effects of decalcification for special stains
Are there negative effects associated with acid decalcification and special stains, for example, AFB, GMS, and PAS (for fungus). All tissues are FFPE. Thanks, Kyle Ayres HT BS Nacogdoches Memorial Hospital ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Effects of decalcification for special stains
Usually not, unless the tissues remain too much time in the decalcifyier, meaning more time than needed to decalcify. For IHC the adverse effect is greater and because of that, when possible, the decalficication should be done with EDTA. René J. --- On Thu, 4/9/09, kyle ayres ayresk...@yahoo.com wrote: From: kyle ayres ayresk...@yahoo.com Subject: [Histonet] Effects of decalcification for special stains To: histonet@lists.utsouthwestern.edu Date: Thursday, April 9, 2009, 3:31 PM Are there negative effects associated with acid decalcification and special stains, for example, AFB, GMS, and PAS (for fungus). All tissues are FFPE. Thanks, Kyle Ayres HT BS Nacogdoches Memorial Hospital ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet]( FE on BM Cores)Effects of decalcification for special stains
Don't forget the effects on decalcified Bone Marrow Cores. Even if you use EDTA decalcification solution, there will be diminished FE staining on Bone Marrow Cores or any other tissue that is decalcified and then stained for FE. Regards,Akemi Akemi Allison-Tacha BS, HT(ASCP)HTL Histology Manager Associated Pathology Medical Group Laboratories 105A Cooper Ct. Los Gatos, CA 95032 Cell: 425.941.4287 E-Mail: akemiat3...@yahoo.com --- On Thu, 4/9/09, Rene J Buesa rjbu...@yahoo.com wrote: From: Rene J Buesa rjbu...@yahoo.com Subject: Re: [Histonet] Effects of decalcification for special stains To: histonet@lists.utsouthwestern.edu, kyle ayres ayresk...@yahoo.com Date: Thursday, April 9, 2009, 1:53 PM Usually not, unless the tissues remain too much time in the decalcifyier, meaning more time than needed to decalcify. For IHC the adverse effect is greater and because of that, when possible, the decalficication should be done with EDTA. René J. --- On Thu, 4/9/09, kyle ayres ayresk...@yahoo.com wrote: From: kyle ayres ayresk...@yahoo.com Subject: [Histonet] Effects of decalcification for special stains To: histonet@lists.utsouthwestern.edu Date: Thursday, April 9, 2009, 3:31 PM Are there negative effects associated with acid decalcification and special stains, for example, AFB, GMS, and PAS (for fungus). All tissues are FFPE. Thanks, Kyle Ayres HT BS Nacogdoches Memorial Hospital ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Used Microtome Parts Available
Are there any used equipment companies out there that would be interested in two used Upper part for disposable blade carrier E ' for a Microm HM325 microtome? Laurie Colbert Huntington Hospital Pasadena, CA (626) 397-8620 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Not Histology:Early Friday and Safe Link RE: 10 most amazing images from Hubble
Hi All, I was deleting my unwanted e-mails last night and came across this link which is amazing and safe. If you haven't previewed this, you may want too. There is a higher power watching over us! http://www.dailymail.co.uk/legacygallery/gallery-9139/Hubble--The-amazing-space-photographs-universe.html?selectedImage=79814 Have a stellar day!Akemi Akemi Allison-Tacha BS, HT(ASCP)HTL Histology Manager Associated Pathology Medical Group Laboratories 105A Cooper Ct. Los Gatos, CA 95032 Cell: 425.941.4287 E-Mail: akemiat3...@yahoo.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Benchmark XT
I just had the same problem with a bad lot of charged slides. It is, indeed, maddening. Joe Nocito jnoc...@satx.rr.com 4/8/2009 5:08 PM another issue could be the mixer blowing too hard. I've had that happen too. JTT - Original Message - From: Kari Bradshaw kbrads...@lcpath.com To: Dana Spears dspe...@mmci.org; histonet@lists.utsouthwestern.edu; Phyllis Thaxton dch...@yahoo.com Sent: Wednesday, April 08, 2009 2:13 PM Subject: RE: [Histonet] Benchmark XT We just recently went through a similar problem with irregular staining, light staining, sometimes no stain of any kind including counterstain,tissue falling off, and a handfull of false negative patient tissues, but positive controls (on the same slide). With two XT's running continuously it taken several days and everyone's help isolating the problemnot to mention several different lots numbers of slidesyikes! Next time I'll blame the slides right off the bat. Kari L. Bradshaw HT(ASCP) Laboratory Manager Lower Columbia Pathologists (360)425-5620 kbrads...@lcpath.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Dana Spears Sent: Wednesday, April 08, 2009 9:24 AM To: histonet@lists.utsouthwestern.edu; Phyllis Thaxton Subject: Re: [Histonet] Benchmark XT I would definitely check your slides - we have found that when we get a bad lot of charged slides, things start washing off on the XT - regardless of detection, antibodies, whatever. It was happening to us on some tissue types and not on others, but a new batch of charged slides did the trick. I've never had to dry 60 min in the oven - 20 does it for us when we aren't having slide issues. Also, if you are using any Sta-on or any adhesive in your waterbath with the charged slides it can cause the slides to sort of repel the tissue and negate the charged effect again certain tissues will fall off, others stay on Dana Spears, HTL(ASCP) Laboratory Manager Methodist Medical Center (309) 672-4930 (office) (309) 255-7214 (cell) (309) 279-3768 (fax) dspe...@mmci.org Phyllis Thaxton dch...@yahoo.com 4/8/2009 8:43:36 AM Is anyone out there having problems with tissue washing off the slides using the Ultraview kit from Ventana? Mainly we are having problems with needle biopsies washing off (prostate, liver). Fixation and processing is always the same 4-6 hours fixation, 3 hour biopsy processing run on VIP processor, cut no thicker than 4 microns, airdried at least 30 minutes, baked at 59-60 for at least one hour prior to staining. The instrument's calibrations have been checked and are fine. We never had this problem with the iView kit using the Nexes, doing pretreatments offline. Any help, ideas, info will be appreciated. Thanks, Phyllis Thaxton HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL 35401 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet NOTICE: This message is a PRIVATE communication. This e-mail may contain confidential or proprietary information that may be considered legally privileged. It is intended only for the named recipient(s). If an addressing or transmission error has misdirected the e-mail, please notify the author by replying to this message. If you are not the named recipient, you are not authorized to use, disclose, distribute, copy, print, or rely on this e-mail, and should immediately delete it from your computer system. Thank you for your assistance with this matter. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Controls needed!
I second that. Marsha is the loveliest person I know. - Original Message - From: Denise Piontek dbpion...@hotmail.com To: histonet@lists.utsouthwestern.edu Sent: Wednesday, April 08, 2009 8:49 PM Subject: RE: [Histonet] Controls needed! Newcomer Supply has been a reliable control source, when necessary the have customized orders. Date: Wed, 8 Apr 2009 07:14:21 -0500 From: jennifer.l.hofec...@vanderbilt.edu To: dknut...@primecare.org; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Controls needed! CC: Hi Deanne, Have you checked with the NSH Control Tissue Bank? The form to request tissue is online on the NSH web page (www.nsh.org). The bank is run by the Quality Control committee in conjunction with the IHCRG. It is a free service to NSH members. You may contact the committee chair, William DeSalvo at wdesalvo@hotmail.com with any specific questions. Have a great week. Jennifer L. Hofecker HT(ASCP) Vanderbilt University Medical Center Division of Neuropathology Nashville, TN ph 615.343.0083 fax 615.343.7089 -Original Message- From: Knutson, Deanne [mailto:dknut...@primecare.org] Sent: Tuesday, April 07, 2009 3:06 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Controls needed! We are looking for H. Pylori control tissue and also GMS/fungus control tissue. Is there anyone out there that might have extra to share? We have good GRAM control blocks that we would be happy to exchange. Please let me know if you can help us out. Thank you! Deanne Knutson Anatomic Pathology Supervisor St. Alexius Medical Center Bismarck, N. Dak. 58506 701-530-6730 Fax 701-530-6735 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _ Rediscover Hotmail®: Get quick friend updates right in your inbox. http://windowslive.com/RediscoverHotmail?ocid=TXT_TAGLM_WL_HM_Rediscover_Updates1_042009___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Many Thanks
I think our problem may very well be the slides. We tried many slides in the past and always came back to the superfrost brand that was most reliable, but I have found different opinions on these slides ;ately. I think it is time to change brands. Thank you all...Hope to see everyone in Birmingham Phyllis Thaxton HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
