[Histonet] Embedding Stamp???
Hi, I am trying to order a tool that I can flatten wavy tissues down at the bottom of the mold for embedding. It looks like a little metal square (kind of) stamp. Do anyone know what it's called and where I can order? Thank you, Judy ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Embedding Stamp???
It's called a tamper and I know Fisher sells them.search embedding tamper on the web site. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartl...@cdc.hhs.gov -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Yeonju Shim Sent: Tuesday, April 14, 2009 9:54 AM To: histonet; histonet-ow...@lists.utsouthwestern.edu Subject: [Histonet] Embedding Stamp??? Hi, I am trying to order a tool that I can flatten wavy tissues down at the bottom of the mold for embedding. It looks like a little metal square (kind of) stamp. Do anyone know what it's called and where I can order? Thank you, Judy ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Embedding Stamp???
They are called tampers and are available at Sakura - 1551 and 1552. They are also available through Cardinal, but I'm not sure the item numbers for Cardinal. Joyce -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Yeonju Shim Sent: Tuesday, April 14, 2009 9:54 AM To: histonet; histonet-ow...@lists.utsouthwestern.edu Subject: [Histonet] Embedding Stamp??? Hi, I am trying to order a tool that I can flatten wavy tissues down at the bottom of the mold for embedding. It looks like a little metal square (kind of) stamp. Do anyone know what it's called and where I can order? Thank you, Judy ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Embedding Stamp???
Some people call it a thumper (I do not like the name at all) and others call it a tissue pressing tool or a tissue flattening tool. Always made of aluminum with a T shape. René J. --- On Tue, 4/14/09, Yeonju Shim shimj...@gmail.com wrote: From: Yeonju Shim shimj...@gmail.com Subject: [Histonet] Embedding Stamp??? To: histonet histonet@lists.utsouthwestern.edu, histonet-ow...@lists.utsouthwestern.edu Date: Tuesday, April 14, 2009, 9:54 AM Hi, I am trying to order a tool that I can flatten wavy tissues down at the bottom of the mold for embedding. It looks like a little metal square (kind of) stamp. Do anyone know what it's called and where I can order? Thank you, Judy ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Embedding Stamp???
It is also called a tamper. Pam Marcum - Original Message - From: Rene J Buesa rjbu...@yahoo.com To: histonet histonet@lists.utsouthwestern.edu, histonet-ow...@lists.utsouthwestern.edu, Yeonju Shim shimj...@gmail.com Sent: Tuesday, April 14, 2009 10:03:34 AM GMT -05:00 US/Canada Eastern Subject: Re: [Histonet] Embedding Stamp??? Some people call it a thumper (I do not like the name at all) and others call it a tissue pressing tool or a tissue flattening tool. Always made of aluminum with a T shape. René J. --- On Tue, 4/14/09, Yeonju Shim shimj...@gmail.com wrote: From: Yeonju Shim shimj...@gmail.com Subject: [Histonet] Embedding Stamp??? To: histonet histonet@lists.utsouthwestern.edu, histonet-ow...@lists.utsouthwestern.edu Date: Tuesday, April 14, 2009, 9:54 AM Hi, I am trying to order a tool that I can flatten wavy tissues down at the bottom of the mold for embedding. It looks like a little metal square (kind of) stamp. Do anyone know what it's called and where I can order? Thank you, Judy ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Avatar Message--Apology to List
Hello Everyone, This crazy program got ahold of my email list and sent this mess out. I apologize to one and all for the inconvenience!! Sorry, Lyn ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Embedding Stamp???
Go to a Pipe shop and look into tobacco tampers. Very useful ... they have a longer handle and don't seem quite as hot as the square Histo tamper. Gary -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Yeonju Shim Sent: Tuesday, April 14, 2009 6:54 AM To: histonet; histonet-ow...@lists.utsouthwestern.edu Subject: [Histonet] Embedding Stamp??? Hi, I am trying to order a tool that I can flatten wavy tissues down at the bottom of the mold for embedding. It looks like a little metal square (kind of) stamp. Do anyone know what it's called and where I can order? Thank you, Judy ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Histonet Digest, Vol 65, Issue 22
Give me a shout in regards to cell pellets TMA's. There are several methods and some are less expensive then others. Have a good one. Lori Beecher is usually difficult to deal with.histonet-requ...@lists.utsouthwestern.edu wrote: Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than Re: Contents of Histonet digest... Today's Topics: 1. Re: TMA for cell pellets? (Bernie Taupin) 2. To all spam gourmets (V. Neubert) 3. RE: I recommend NOT opening that Attachment: (Hugh Luk) 4. RE: TMA for cell pellets? (Hartson, Louise) 5. Re: Formula 83 users? (Rene J Buesa) 6. Re: Negative IHC controls (Rene J Buesa) 7. RE: Formula 83 users? (Blazek, Linda) 8. Re: Spyware from an Attachment: (Geoff McAuliffe) 9. Formula 83-Thank you! (Jacqueline Farnsworth) 10. HISTOS 5 processor (FU,DONGTAO) 11. Position Open In Mass (Alyssa Peterson) 12. Re: HISTOS 5 processor (Robert Schoonhoven) 13. Travelling Histotechs (Robert Schoonhoven) 14. Regional Sales Manager position- West Coast (kris.caldw...@leica-microsystems.com) 15. New lab, need some info (Putnam, Jodi) -- Message: 1 Date: Wed, 8 Apr 2009 22:50:26 -0700 (PDT) From: Bernie Taupin bernietau...@ymail.com Subject: Re: [Histonet] TMA for cell pellets? To: Thom Jensen tissuear...@hotmail.com, louise_hart...@urmc.rochester.edu, histonet@lists.utsouthwestern.edu Message-ID: 338565.20208...@web43513.mail.sp1.yahoo.com Content-Type: text/plain; charset=iso-8859-1 Recently, someone mentioned putting a library of protocols online, perhaps in the HistoNet archives. I'd like to second that emotion if its possible to make happen... What a great resource that would be! From: Thom Jensen tissuear...@hotmail.com To: louise_hart...@urmc.rochester.edu; histonet@lists.utsouthwestern.edu Sent: Thursday, April 9, 2009 1:27:18 AM Subject: [Histonet] TMA for cell pellets? Have you tried to embed your cell pellets into a tissue microarray? Two companies I would recommend: www.arraymold.com www.beecherinstruments.com Two of the best TMA products on the market. cheers, Date: Thu, 2 Apr 2009 12:05:39 -0400 From: louise_hart...@urmc.rochester.edu To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Embedding cell pellets I am looking for a protocol for embedding cell pellets in paraffin. Thanks, Louise Louise Hartson, BA Senior Technical Associate University of Rochester louise_hart...@urmc.rochester.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _ Hotmail® is up to 70% faster. Now good news travels really fast. http://windowslive.com/online/hotmail?ocid=TXT_TAGLM_WL_HM_70faster_032009___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Message: 2 Date: Thu, 09 Apr 2009 10:07:54 +0200 From: V. Neubert histonet.nos...@vneubert.com Subject: [Histonet] To all spam gourmets To: histonet@lists.utsouthwestern.edu Message-ID: 49ddacda.8040...@vneubert.com Content-Type: text/plain; charset=ISO-8859-1; format=flowed Please, stop spamming this list. Comment if you know something useful. Otherwise keep it. I don't mind what you had for breakfast in June 1st last year or your opinion on my clothes' colour. Believe it or not, there IS a real life ( link: http://en.wikipedia.org/wiki/Real_life_(reality) ), and maybe you should check it out - it has way more to offer than waiting for responses to mock about. Thanks. Valentin PS: What about a moderated board? Every inappropriate comment on HISTO TOPIC could be moved or even deleted, except in the spamming forum which can be found on nearly every board on the net. -- Message: 3 Date: Wed, 8 Apr 2009 22:13:22 -1000 From: Hugh Luk hlu...@msn.com Subject: RE: [Histonet] I recommend NOT opening that Attachment: To: histonet histonet@lists.utsouthwestern.edu Message-ID: snt106-w30443621932d763b828123c3...@phx.gbl Content-Type: text/plain; charset=iso-8859-1 Fellow histology and related fields professionals, I'm not opening this. From a call name of u know (u_deserve_2_k...@yahoo.com). The last time I opened something like this, from
RE: [Histonet] Embedding Stamp???
Ack! You beat me to it ;-) We use pipe nails which come from a local pipe store and are very inexpensive. They work well for prostate and other core biopsies. Bill B. At 7:12 AM -0700 4/14/09, Martin, Gary wrote: Go to a Pipe shop and look into tobacco tampers. Very useful ... they have a longer handle and don't seem quite as hot as the square Histo tamper. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] TBS vs PBS
Hello all, Would proteinase K diluted in PBS be any different then PK diluted in Tris buffer when used for a fluorescent antibody test? I'm having trouble duplicating a published testing method and the only difference is PK diluted in TBS instead of PBS. Thanks in advance for all the wonderful information from all. Roger Roger Charles Microbiologist Pennsylvania Veterinary Laboratory 2305 N Cameron St Harrisburg, PA 17110 717-787-8808 rchar...@state.pa.usmailto:rchar...@state.pa.us No trees were hurt in the sending of this email, However many electrons were severely inconvenienced! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RELIA Histology Job Alert Histology tech needed in Atlanta!
Hi Histonetters! I hope everyone is having a great day! I am excited to tell you about a brand new position in a private pathology lab in the Atlanta area. I know this client very well and it is an excellent place to work. They are an established lab with a growing practice. The supervisor is great to work with and the salary and benefits are very competitive for the Atlanta area. This is a permanent full time dayshift position. The schedule is M-F dayshift. My client is in need of an ASCP certified tech with several years of experience. This person will need strong cutting and embedding skills and Ability to work under pressure, attention to detail, excellent fine motor skills for the cutting work required for the tissue, a very light touch when handing delicate biopsies and tissue and strong laboratory skills. While the core schedule is M-F dayshift some flexibility in days and hours is preferred. If you or anyone you know might be interested in this position please contact me. I can be reached toll free at 866-607-3542 or rel...@earthlink.net Have a great day!! I Thank You! Pam Barker President RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 Toll Free: (866)607-3542 FAX: (407)678-2788 E-mail: mailto:rel...@earthlink.net rel...@earthlink.net mailto:rel...@earthlink.net mailto:rel...@earthlink.net http://home.earthlink.net/~relia1 http://home.earthlink.net/~relia1 http://www.myspace.com/pamatrelia www.myspace.com/pamatrelia http://www.myspace.com/pamatrelia http://www.myspace.com/pamatrelia ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] 5 Reasons
Please help me out here I am suppose to post 5 Reasons to become a histotech in our lab. It is a competition for lab week. Each department has to post 5 reasons you should work in their department. I am looking for some great answers Thanks, Cindy ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] (no subject)
Is anyone using their target retrieval for dako more than 1 time? Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] decaling bones
We are having the hardest time decaling bones. Femurs and tibias. Is there such as thing as overdecaling and the bone becoming hard again? Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 1 Children's WaySlot 820 Little Rock, AR 72202 phone 501.364.4240 fax501.364.3155 visit us on the web at:www.archildrens.org ** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Blocking
About to use the antibody anti-connexin 43. On the data sheet it tells me to block using 5% milk, so it's casein blocking. In 1992 Dave Tacha published a paper (J. Histotech. 15. 132-137. Casein reduces non-specific background staining in immunolabelling techniques), regarding casein blocking where he recommended using it at 5%. Later however, Mary Vaughan on Histonet suggested reducing the concentration, after a series of experiments, to 0.03% for 30 minutes. What's the current view on casein blocking, concentration, time etc. Ian. Dr. Ian Montgomery, Histotechnology, I.B.L.S. Support Unit, Thomson Building, University of Glasgow, Glasgow, G12 8QQ. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] F4/80
Hi Histonet, We have a working IHC protocol for F4/80 on mouse tissues. However, when we try to switch this procedure to IF it doesn't work. Would anyone please have any suggestions as to why this won't work? The pretreatment that we use is trypsin at 37 degrees for 15 min. Does this enzyme inhibit the Cy5 which is the secondary fluorophore that we're using? Any advice/help is greatly appreciated! Thanks! Anne ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Research/Pharma Position In Mass
*Hi,* * * *My name is Alyssa Peterson, and I am the director of Lab/Research recruitment for Allied Search Partners. I wanted to follow up with everyone on Histonet about a position that I am currently setting up interviews for. Please read over the job description and let me know if you are interested in exploring this opportunity and send me an updated resume. If not, we do offer to pay up to $1000 as a referral bonus, so please feel free to forward this to whomever you feel fit. * ** *LOCATION* 20 miles Northwest of Boston, MA *TITLE/POSITION* * * Status: Exempt. Regular, full-time. Work Shift: 8:00am - 5:00pm, M-F, and as required. Flex hours as necessary. Weekly schedule may be Tuesday – Saturday or Sunday – Thursday. *QUALIFICATIONS* B.S. in Life Sciences or equivalent experience. Animal handling restraint experience with both large and small animals required. Must be experienced with all methods of dose administration and biological sample collection techniques in all laboratory species. Self-disciplined, independent worker who can work well on his/her own and with a team. Must possess good time- management skills. Ability to work in a fast-paced environment. Attention to detail. Ability to lift 50 lbs. Must be able to work late nights for study performance and weekends. Prior experience performing necropsies needed. *RESPONSIBILITIES* Conduct In Vivo/Pharma testing under appropriate guidelines (FDA, EPA, GLP, ISO, OECD). Prepare testing solutions, perform dose administration (using IM, IP, ID, IV, SQ, topical, nasal and oral gavage), and perform blood collection on small animals (retro orbital, IC in mice rats, and ear vein in rabbits) and large animals (cephalic, saphenous, IC, etc.) Handle mice, rats, hamster, rabbits, guinea pigs, cats, dogs, swine, sheep, goats and primates. Performs multiple projects with the emphasis on small animal PK/TOX studies and manages all aspects of these studies. These would include the dosing, bleeding, processing of the samples taken and all data associated with these studies. Will work closely with the Study Directors as it pertains to the studies. Daily responsibilities will included but are not limited to clinical observation, body weights, food consumption, ECG’s, etc. Generates and maintains all paperwork associated with each project. Assist with limited animal care activities, such as feeding, water and bedding/cage changes as necessary. Preparation and review of data packages, utilized in the collection of study raw data. * * Maintain conformance with quality mission statement, goals and values. Adhere to compliance with ISO 17025 certification through the laboratories and operations. Ensuring Compliance with health and safety programs, radiation and other regulatory programs.** * * Assist management in special projects, as requested. * * -- Alyssa Peterson Allied Search Partners O: 770.621.2639 ext. 4 F: 770.621.2640 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] 5 Reasons
How about the ability to diversify among several curricula : special staining, IHC, sectioning, special procedures (muscle, nerve biopsies), Electron Microscopy, Immunoflourescence... From: histonet-boun...@lists.utsouthwestern.edu on behalf of Cindy DuBois Sent: Tue 4/14/2009 11:52 AM To: Histonet Subject: [Histonet] 5 Reasons Please help me out here I am suppose to post 5 Reasons to become a histotech in our lab. It is a competition for lab week. Each department has to post 5 reasons you should work in their department. I am looking for some great answers Thanks, Cindy ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet === The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. === ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Tissue Tek II- last one
Almost have the lab boxed up but I have 1Tissue Tek II staining tray with 12 staining dishes along with 14 more other staining dishes left. Cathy A. Mayton Wasatch Histo Consultants, Inc. 775-625-4425 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Tissue Tek II gone
Thanks for all the inquiries but the Tissue Tek II staining tray and all the extra dishes are gone. Cathy A. Mayton Wasatch Histo Consultants, Inc. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Xylene substitute
Formula 83, like xylene and many xylene substitutes, is categorized as flammable according to its MSDS and, thus, must be handled as a hazardous waste in accordance with EPA regulations, including monitoring its exposure, storage, and disposal. Peter Peter G. Baldwin Director of Sales, Marketing Business Development pbald...@micronenvironmental.com Micron Environmental Industries, Inc. Green Chemistry for LifeSM www.MicronEnvironmental.com 1221 Cameron Street Alexandria, VA 22314 703-548-2776 703-548-7988/Fax ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] re: decaling bone
I don't think the bone matrix will re-calcify with normal bone histo solutions. What exactly are you using to decalcify the tissue? Hardening can occur at many steps following decal. I stopped using a commercial decalcifying agent (which consisted of formic acid with chelating agents added) because my tissue samples would harden more rapidly after dehydration, clearing and infiltration (paraffin) and wouldn't infiltrate properly. Not sure if it was the chelating additives or not, but I went back to using Formic A solution with 5-10% formic depending on the type of tissue. Matt ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Lab Week-Histology Trivial or Fun Facts
Hello, I was wondering if anyone has some histo trivial-fun facts to share for Lab Week? I remember a supervisor told me long ago that she was told while in training, that if you took a hard boilded egg and sectioned it at 2 microns you would have enough sections to cover a football field. Has anyone ever heard that one before? Can anyone contribute any others? We are trying to come up with some games for lab week. Lori ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Lab Week-Histology Trivial or Fun Facts
Lori Trivia but may not be fun. A fact re electron microscope. Glass knives have been used in electron microscopy since around the 1950s for preparation of thin sections. (The best glass was from antique windows. As glass is a viscous material that is constantly flowing, antique glass has less strain lines in it.) However this was not the first instance in which glass knives were used. I believe the first use was by Gutav Mann. In his book on histologic technique in 1902 he recommended the use of glass instead of steel knives in those instances when tissues were to be examined for iron, as he was afraid that steel knives used during cutting would contaminate the tissue. Barry -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Disher Lori Sent: Tuesday, April 14, 2009 2:13 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Lab Week-Histology Trivial or Fun Facts Hello, I was wondering if anyone has some histo trivial-fun facts to share for Lab Week? I remember a supervisor told me long ago that she was told while in training, that if you took a hard boilded egg and sectioned it at 2 microns you would have enough sections to cover a football field. Has anyone ever heard that one before? Can anyone contribute any others? We are trying to come up with some games for lab week. Lori ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Embedding Stamp???
Histonet friends, Try using the hex head of a 3/8 inch diameter 1-2 1/2 long bolt. This works quite well and costs about 15 cents at the hardware store. J.B.McCormick, M.D. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Yeonju Shim Sent: Tuesday, April 14, 2009 8:54 AM To: histonet; histonet-ow...@lists.utsouthwestern.edu Subject: [Histonet] Embedding Stamp??? Hi, I am trying to order a tool that I can flatten wavy tissues down at the bottom of the mold for embedding. It looks like a little metal square (kind of) stamp. Do anyone know what it's called and where I can order? Thank you, Judy ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Lab Week-Histology Trivial or Fun Facts
Histology world has some cute games and fun facts: http://www.histology-world.com/ -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet- boun...@lists.utsouthwestern.edu] On Behalf Of Disher Lori Sent: Tuesday, April 14, 2009 3:13 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Lab Week-Histology Trivial or Fun Facts Hello, I was wondering if anyone has some histo trivial-fun facts to share for Lab Week? I remember a supervisor told me long ago that she was told while in training, that if you took a hard boilded egg and sectioned it at 2 microns you would have enough sections to cover a football field. Has anyone ever heard that one before? Can anyone contribute any others? We are trying to come up with some games for lab week. Lori ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Lab Week-Histology Trivial or Fun Facts
Hi All, No. I never heard the hard boiled egg sectioning fact. 1. Match the baby picture to the tech. 2. Gather information on if one weren't a histotech what would one do? Create a form and have the techs match names to the profession. It could be fun. I participated in another lab once and the techs were funny. If I weren't a histotech I'd travel on the speech circuit. That particular person talked all day, literally. OR you can come up with professions based on the techs personality and have everyone guess on the form. Who ever get the most correct gets $5.00 Wawa card or something. 3. Unscramble laboratory terms from all labs including clinical. 4.Double check this info, Who developed the diamond knife for electron microscopy AND improved the ultramicrotome Answer: Humberto Fernandez-Moran--- Venezuela Stephanie D. Rivera Safety Assessment Department GlaxoSmithKline 709 Swedeland RD King of Prussia, PA 19406 phone: 610-270-7340 fax: 610-270-7202 Disher Lori lori.dis...@hcahealthcare.com Sent by: histonet-boun...@lists.utsouthwestern.edu 14-Apr-2009 15:12 To histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu cc Subject [Histonet] Lab Week-Histology Trivial or Fun Facts Hello, I was wondering if anyone has some histo trivial-fun facts to share for Lab Week? I remember a supervisor told me long ago that she was told while in training, that if you took a hard boilded egg and sectioned it at 2 microns you would have enough sections to cover a football field. Has anyone ever heard that one before? Can anyone contribute any others? We are trying to come up with some games for lab week. Lori ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Embedding Stamp???
Sakura used to sell items called Tissue Tampers just for this purpose in different sizes. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Yeonju Shim Sent: Tuesday, April 14, 2009 8:54 AM To: histonet; histonet-ow...@lists.utsouthwestern.edu Subject: [Histonet] Embedding Stamp??? Hi, I am trying to order a tool that I can flatten wavy tissues down at the bottom of the mold for embedding. It looks like a little metal square (kind of) stamp. Do anyone know what it's called and where I can order? Thank you, Judy -- ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Job opening in East Tennessee
Blount Memorial Hospital in Maryville, TN has a histology technician position open Monday thru Friday 7-3:30. We are located just minutes from the beautiful Smoky Mountains National Park and experience 4 wonderful seasons! All types of outdoor activities are possible. Maryville is host to the annual Foothills Fall Festival with top name entertainment and crafts, and just 30 minutes from Knoxville's culture events and entertainment, as well as UT football, basketball, etc. Applicants must meet the educational and training requirements necessary for certification by the American Society of Clinical Pathology as a Histology Technician or have experience equal to certification. General histology experience preferred. Must demonstrate competency and successfully complete the on-the-job orientation through the histology section of the laboratory. Performs all duties of a Histology Technician and other duties as assigned. Technically, it is listed as part time, but there will be 40 hrs/wk needed. Good benefits are offered. Anyone interested please visit our website at blountmemorial.org. to fill out an application and attach a resume. Or, you may just send me a resume. Thanks, Renee Grow, BA., HT (ASCP) rg...@bmnet.com Histology Supervisor Blount Memorial Hospital 907 E. Lamar Alexander Pkwy. Maryville, TN 37804-5016 (865) 977-4744 (865) 977-5766 Fax This communication may contain protected health information (PHI) that is legally protected from inappropriate disclosure by the Privacy Standards of the Health Insurance Portability and Accountability Act (HIPAA) and relevant Tennessee Laws. If you are not the intended recipient, please note that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this message in error, you should notify the sender immediately by telephone or by return e-mail and delete this message from your computer. Direct questions to the Blount Memorial Hospital Privacy Officer at 865-977-4675. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] 2009 MO Society for Histotechnology Symposium
Mark your calendar for this years MO Society for Histotechnology Symposium to be held at the beautiful Lake of the Ozarks, Port Arrowhead Resort on May 29-30, with a vendor reception and MSH Quarterly Business Meeting on Thursday evening, May 28. Our program is as follows: Thursday, May 28: 6:00 pm - 8:00 pm - Registration and Exhibit Hall Open 8:00 pm - Enjoy the Portside Lounge with friends or attend the MSH Quarterly Business Meeting Friday, May 29: 7:00 - 7:45 am - Registration 7:45 am - Welcome - Amanda Kelley, MSH President 8:00 - 9:30 - Brave New World: Introduction to Molecular Pathology, Part 1 - Dr. Thomas Haas, DO, FASCP 9:30 - 10:00 - Exhibit Hall Open 10:00 - 11:15 - Brave New World: Introduction to Molecular Pathology, Part 2 - Dr. Thomas Haas, DO, FASCP 11:30 - 12:30 - Green Histology - Laurence Patton BS, HT(ASCP) 12:30 - 1:15 - Lunch on your own 1:30 - 5:00 Workshop 1 - Muscle Biopsies: Gross Room to Reading Room - Konnie Zeitner, HT(ASCP)HTL SLS Workshop 2 - The Histology Workout: Getting Lean in the Lab - Christine Charlie Dorner, HT(ASCP)QIHC Workshop 3 - Methyl Methacrylate - Why and How? - Jack Ratliff, BA 6:00 - 8:00 Tropic Island Dinner Cruise (Cash Bar) Saturday, May 30 7:00 - 7:45 am - Registration 7:45 - Welcome - JP Rey, MSH Vice-President 8:00 - 8:45 - Mohs at a Glance, The Benefits of Moh's Surgery - Gina Rodriguez, HT(ASCP) 8:45 - 10:00 - Where We are and Where we have been with Tissue Processing, Part 1 - Christine Charlie Dorner, HT(ASCP)QIHC 10:00 - 10:30 - Exhibit Hall Open 10:30 - 12:00 - Where We are and Where we have been with Tissue Processing, Part 2 - Christine Charlie Dorner, HT(ASCP)QIHC 12:00 - 1:30 - MSH Awards lunch with Exhibitors 1:30 - 4:30 Workshop 4 - The Clinical, Technical, and Financial Benefits of Multi-antigen Immunostaining (MAIS) Procedures - Joseph D. Myers, MS, CT(ASCP) Workshop 5 - Tissue Identification for the Histotechnologist - Dr. Thomas Haas, DO, FASCP Workshop 6 - The What, When, Where, and How of Disaster Preparedness - Sylvia Jackson Casey, HT(ASCP), MA/MA All workshops are CEU approved by NSH. For complete information and a pdf copy of the brochure, visit www.missouri-histo.org or email Amanda Kelley kelleypa...@charter.net or JP Rey jp10...@hotmail.com Hotel reservation deadline is April 27, 2009, and the toll free number is 1-800-532-3575. Let them know you're attending the MSH symposium for a reduced room rate. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] decaling bones
No, once it is decalcified, that's it! René J. --- On Tue, 4/14/09, Horn, Hazel V hor...@archildrens.org wrote: From: Horn, Hazel V hor...@archildrens.org Subject: [Histonet] decaling bones To: histonet@lists.utsouthwestern.edu Date: Tuesday, April 14, 2009, 12:04 PM We are having the hardest time decaling bones. Femurs and tibias. Is there such as thing as overdecaling and the bone becoming hard again? Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 1 Children's WaySlot 820 Little Rock, AR 72202 phone 501.364.4240 fax501.364.3155 visit us on the web at:www.archildrens.org ** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Sales Account Executive- Chicagoland
This person will need to be based in Northern Illinois or Southern Wisconsin. Leica Microsystems is a leading global designer and producer of innovative high-tech precision optics systems for the analysis of microstructures. It is one of the market leaders in each of the fields of Microscopy, Confocal Laser Scanning Microscopy, Imaging Systems, Specimen Preparation and Medical Equipment. Comprising nine manufacturing facilities in seven countries, sales and service companies in 20 countries and an international network of dealers, the company is represented in over 100 countries, Leica Microsystems is a leading global designer and producer of innovative high-tech precision optics systems for the analysis of microstructures. It is one of the market leaders in each of the fields of Microscopy, Confocal Laser Scanning Microscopy, Imaging Systems, Specimen Preparation and Medical Equipment. To achieve Leica sales and profitability goals within an assigned territory through the implementation of aggressive, direct end-user selling techniques. Promote Leica as the Histology market leader in quality and innovation. - Achieve monthly, quarterly, annual and strategic product mix sales goals for the territory. - Plan and schedules face-to-face account calls to current and potential end-users of Leica products. - Identify and develop key accounts in the territory. - Manage assigned national accounts within territory requiring corporate coordination to enable closure and compliance of contracts. - Install/set-up instrumentation in customer laboratories. Perform demonstrations. Maintain demonstration equipment in a clean and operational manner. Train customers on the use of instrumentation. Prepare monthly territory status reports to Regional Sales Manager (including, but not limited to, Target Account Lists, Won/Lost accounts, Forecasts, expense reports and travel calendars). Maintain current knowledge of competition and market through study of competitive marketing information, competitive literature, and field surveillance of competition. Requirements - BA/BS in Life Sciences or equivalent - 1-3 years sales experience in capital equipment - Demonstrated success in selling - Experience selling Microtomes, cryostats, or autostainers - Understanding of Histology marketplace or a related discipline - 1-3 years Histology laboratory experience in clinical, research or industrial setting desirable but not required - Histotechnologist background is a plus - Good selling, negotiating, closing and account management skills - Self motivated - Goal oriented, results driven - Good interpersonal skills - Ability to comprehend scientific applications/markets - Good time management, good organizational skills - Works independently but able to interact as a team member - Good communications skills Proficient in computer skills (Excel, Word, PowerPoint, Lotus Notes, SAP) Send your resume to- kris.caldw...@leica-microsystems.com Leica offers competitive salary, benefits including medical, dental, vision, prescription, long-term care, life insurance, STD, LTD, and 401 (k). Please, no solicitations from 3rd Party Vendors. Thank you. Kris Caldwell Human Resources Recruiter Leica Microsystems, Inc. 2345 Waukegan Road Bannockburn, IL 60015 www.leica-microsystems.com kris.caldw...@leica-microsystems.com 847-405-5432 - phone 847-236-3035 - fax 847-323-6169- cellular __ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email __ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] TBS vs PBS
I am not sure of the effect of buffer type on the protease but it might be possible that the proteinase K is the problem. My experience is that they can vary between lots. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Charles, Roger Sent: Wednesday, 15 April 2009 12:41 AM To: Histonet (histonet@lists.utsouthwestern.edu) Subject: [Histonet] TBS vs PBS Hello all, Would proteinase K diluted in PBS be any different then PK diluted in Tris buffer when used for a fluorescent antibody test? I'm having trouble duplicating a published testing method and the only difference is PK diluted in TBS instead of PBS. Thanks in advance for all the wonderful information from all. Roger Roger Charles Microbiologist Pennsylvania Veterinary Laboratory 2305 N Cameron St Harrisburg, PA 17110 717-787-8808 rchar...@state.pa.usmailto:rchar...@state.pa.us No trees were hurt in the sending of this email, However many electrons were severely inconvenienced! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet * This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] decaling bones
The bone appears soft and easy to cut with a razor blade but after processing the tissue is as hard as brick. Any suggestions? Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 1 Children's WaySlot 820 Little Rock, AR 72202 phone 501.364.4240 fax501.364.3155 visit us on the web at:www.archildrens.org From: Rene J Buesa [mailto:rjbu...@yahoo.com] Sent: Tuesday, April 14, 2009 4:02 PM To: histonet@lists.utsouthwestern.edu; Horn, Hazel V Subject: Re: [Histonet] decaling bones No, once it is decalcified, that's it! René J. --- On Tue, 4/14/09, Horn, Hazel V hor...@archildrens.org wrote: From: Horn, Hazel V hor...@archildrens.org Subject: [Histonet] decaling bones To: histonet@lists.utsouthwestern.edu Date: Tuesday, April 14, 2009, 12:04 PM We are having the hardest time decaling bones. Femurs and tibias. Is there such as thing as overdecaling and the bone becoming hard again? Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 1 Children's WaySlot 820 Little Rock, AR 72202 phone 501.364.4240 fax501.364.3155 visit us on the web at:www.archildrens.org ** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] 5 Reasons
Cindy, A much respected and knowledgeable Histotech (who is now retired) said: Histotechnology - you either hate it or love it with a passion! Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cindy DuBois Sent: Wednesday, 15 April 2009 1:52 AM To: Histonet Subject: [Histonet] 5 Reasons Please help me out here I am suppose to post 5 Reasons to become a histotech in our lab. It is a competition for lab week. Each department has to post 5 reasons you should work in their department. I am looking for some great answers Thanks, Cindy ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet * This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] decaling bones
Hazel Processing using alcohol then xylene? If so then suspect too long in alcohol or using xylene is your major problem with paraffin wax a close second. Can use chloroform as an intermediary agent and bone will not harden as much. If you must use xylene, try to soak in a mixture of xylene:paraffin wax (1:1) at room temperature for at least a few hours. This allows some wax to penetrate into the tissue and cuts down time in paraffin wax. Barry -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V Sent: Tuesday, April 14, 2009 4:17 PM To: rjbu...@yahoo.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] decaling bones The bone appears soft and easy to cut with a razor blade but after processing the tissue is as hard as brick. Any suggestions? Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 1 Children's WaySlot 820 Little Rock, AR 72202 phone 501.364.4240 fax501.364.3155 visit us on the web at:www.archildrens.org From: Rene J Buesa [mailto:rjbu...@yahoo.com] Sent: Tuesday, April 14, 2009 4:02 PM To: histonet@lists.utsouthwestern.edu; Horn, Hazel V Subject: Re: [Histonet] decaling bones No, once it is decalcified, that's it! René J. --- On Tue, 4/14/09, Horn, Hazel V hor...@archildrens.org wrote: From: Horn, Hazel V hor...@archildrens.org Subject: [Histonet] decaling bones To: histonet@lists.utsouthwestern.edu Date: Tuesday, April 14, 2009, 12:04 PM We are having the hardest time decaling bones. Femurs and tibias. Is there such as thing as overdecaling and the bone becoming hard again? Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 1 Children's WaySlot 820 Little Rock, AR 72202 phone 501.364.4240 fax501.364.3155 visit us on the web at:www.archildrens.org ** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Histo Trivia
The NSH journal that just came had an article on the history of Histology. I am only halfway through the article but their were some interesting facts that you could probably use. I also want to thank all of you who helped me put together 5 reasons to work in histology. I am paring the list down to 5 and will post them here probably tomorrow. Cindy ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] (no subject)
I have used DAKO Target Retrieval in the past and it is definitely possible to re-use the solution. The important thing to do is to check the pH each day before use as this is the best indicator that the solution needs to be changed. It has bee an while since I used it but from my shaky memory you should get at least a week from the solution. regards Tony Reilly Chief Scientist Anatomical Pathology Pathology Queensland Level 1, Building 15 Princess Alexandra Hospital Ipswich Rd, Woolloongabba Q 4102 Australia Ph: 07 32402412 Fax:07 32402930 tony_rei...@health.qld.gov.au jeff jlhow...@yrmc.org 15/04/2009 1:55 am Is anyone using their target retrieval for dako more than 1 time? Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This email, including any attachments sent with it, is confidential and for the sole use of the intended recipient(s). This confidentiality is not waived or lost, if you receive it and you are not the intended recipient(s), or if it is transmitted/received in error. Any unauthorised use, alteration, disclosure, distribution or review of this email is strictly prohibited. The information contained in this email, including any attachment sent with it, may be subject to a statutory duty of confidentiality if it relates to health service matters. If you are not the intended recipient(s), or if you have received this email in error, you are asked to immediately notify the sender by telephone collect on Australia +61 1800 198 175 or by return email. You should also delete this email, and any copies, from your computer system network and destroy any hard copies produced. If not an intended recipient of this email, you must not copy, distribute or take any action(s) that relies on it; any form of disclosure, modification, distribution and/or publication of this email is also prohibited. Although Queensland Health takes all reasonable steps to ensure this email does not contain malicious software, Queensland Health does not accept responsibility for the consequences if any person's computer inadvertently suffers any disruption to services, loss of information, harm or is infected with a virus, other malicious computer programme or code that may occur as a consequence of receiving this email. Unless stated otherwise, this email represents only the views of the sender and not the views of the Queensland Government. ** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
FW: [Histonet] (no subject)
I also used Dako's Target Retrieval buffer in the past and it was also possible to dilute further up to 1:20 or 1:40 (instead of 1:10) with good results. Young -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Anthony Reilly Sent: Wednesday, 15 April 2009 10:31 AM To: histonet@lists.utsouthwestern.edu; jeff Subject: Re: [Histonet] (no subject) I have used DAKO Target Retrieval in the past and it is definitely possible to re-use the solution. The important thing to do is to check the pH each day before use as this is the best indicator that the solution needs to be changed. It has bee an while since I used it but from my shaky memory you should get at least a week from the solution. regards Tony Reilly Chief Scientist Anatomical Pathology Pathology Queensland Level 1, Building 15 Princess Alexandra Hospital Ipswich Rd, Woolloongabba Q 4102 Australia Ph: 07 32402412 Fax:07 32402930 tony_rei...@health.qld.gov.au jeff jlhow...@yrmc.org 15/04/2009 1:55 am Is anyone using their target retrieval for dako more than 1 time? Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This email, including any attachments sent with it, is confidential and for the sole use of the intended recipient(s). This confidentiality is not waived or lost, if you receive it and you are not the intended recipient(s), or if it is transmitted/received in error. Any unauthorised use, alteration, disclosure, distribution or review of this email is strictly prohibited. The information contained in this email, including any attachment sent with it, may be subject to a statutory duty of confidentiality if it relates to health service matters. If you are not the intended recipient(s), or if you have received this email in error, you are asked to immediately notify the sender by telephone collect on Australia +61 1800 198 175 or by return email. You should also delete this email, and any copies, from your computer system network and destroy any hard copies produced. If not an intended recipient of this email, you must not copy, distribute or take any action(s) that relies on it; any form of disclosure, modification, distribution and/or publication of this email is also prohibited. Although Queensland Health takes all reasonable steps to ensure this email does not contain malicious software, Queensland Health does not accept responsibility for the consequences if any person's computer inadvertently suffers any disruption to services, loss of information, harm or is infected with a virus, other malicious computer programme or code that may occur as a consequence of receiving this email. Unless stated otherwise, this email represents only the views of the sender and not the views of the Queensland Government. ** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _ This email has been scanned for the Sydney South West Area Health Service by the MessageLabs Email Security System. SSWAHS regularly monitors emails and attachments to ensure compliance with the NSW Government's Electronic Messaging Policy. This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please destroy it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of the Sydney South West Area Health Service. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Lab Week-Histology Trivial or Fun Facts LONG OFF TOPIC
Hi in grad school taking microanatomy and pathology classes, 2 that I heard are this: The surface area of all the alveoli in the lungs of an adult is between 40-70 square meters. That seems reasonable in having a 40-70 square meter surface (where all gas exchange takes place) represent all the gas exchange in lungs. Have seen that figure numerous times so while can't test it, can believe it. The other one that I also can't test and is hard to believe is that the sum total length of all vessels (large small, artery vein down to every single capillary) in one adult measures about 100,000 kilometers (62,000 miles). Again there are many disparate medical and anatomical references so either all right or all wrong. The 2 micron sectioned egg I don't believe. (1) There are 25,400 microns in an inch. A 2 inch long egg is about 50,000 microns long. At 2 microns per section thats about 25,000 egg sections. Even is each section is 2 square inches (that's generous since each end isn't close to 2 squre inches in area), thats 100,000 square inches. At 1,296 square inches per square yard, that's about 40 square yards which is far short of a football field (100 yards x 53 yards). (2) If you calculate the volume of a solid rectangle covering a football feild that is 100 yards x 53 yards x 2 microns and of course converting all to yards or microns, the answer is a specific volume. If you take the volume of an ellipsoid which is four thirds times pi times a times b times c with a, b and c being the lenggth of the 3 axis of the ellipsoid, and using approximate measurements for the egg, I come up with far , far less volume in egg than in the rectangular solid covering football field. (3) This is a classical calculus definte integral washer problem. Whether this egg as an ellipsoid is scalene, oblate or prolate, integrating volume over the limits of integration gives me much, much less volume than is needed to cover a football field 2 microns thick. Have tried all 3 methods and converting everything to microns or yards using scientific notation. So 6 calculations. Everytime I come up somewhere close to the area of 2 micron slices covering approximately 1/100 of the football field. Unless my math is all wrong, or this is a humongous, enormous ostrich and not chicken egg. Ray Raymond Koelling PhenoPath Labs Seattle, WA - Original Message - From: Disher Lori lori.dis...@hcahealthcare.com To: histonet@lists.utsouthwestern.edu Sent: Tuesday, April 14, 2009 12:12:40 PM GMT -08:00 US/Canada Pacific Subject: [Histonet] Lab Week-Histology Trivial or Fun Facts Hello, I was wondering if anyone has some histo trivial-fun facts to share for Lab Week? I remember a supervisor told me long ago that she was told while in training, that if you took a hard boilded egg and sectioned it at 2 microns you would have enough sections to cover a football field. Has anyone ever heard that one before? Can anyone contribute any others? We are trying to come up with some games for lab week. Lori ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: FW: [Histonet] (no subject)
As per Young's response, I have never diluted the solution further but found that if there was some drop in the level of the solution due to evaporation it could be topped up with deionised water as long as the pH was not changed. This was on the advice of the DAKO rep at the time. regards Tony Reilly Chief Scientist Anatomical Pathology Pathology Queensland Level 1, Building 15 Princess Alexandra Hospital Ipswich Rd, Woolloongabba Q 4102 Australia Ph: 07 32402412 Fax:07 32402930 tony_rei...@health.qld.gov.au Young Kwun kw...@email.cs.nsw.gov.au 15/04/2009 1:29 pm I also used Dako's Target Retrieval buffer in the past and it was also possible to dilute further up to 1:20 or 1:40 (instead of 1:10) with good results. Young -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Anthony Reilly Sent: Wednesday, 15 April 2009 10:31 AM To: histonet@lists.utsouthwestern.edu; jeff Subject: Re: [Histonet] (no subject) I have used DAKO Target Retrieval in the past and it is definitely possible to re-use the solution. The important thing to do is to check the pH each day before use as this is the best indicator that the solution needs to be changed. It has bee an while since I used it but from my shaky memory you should get at least a week from the solution. regards Tony Reilly Chief Scientist Anatomical Pathology Pathology Queensland Level 1, Building 15 Princess Alexandra Hospital Ipswich Rd, Woolloongabba Q 4102 Australia Ph: 07 32402412 Fax:07 32402930 tony_rei...@health.qld.gov.au jeff jlhow...@yrmc.org 15/04/2009 1:55 am Is anyone using their target retrieval for dako more than 1 time? Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This email, including any attachments sent with it, is confidential and for the sole use of the intended recipient(s). This confidentiality is not waived or lost, if you receive it and you are not the intended recipient(s), or if it is transmitted/received in error. Any unauthorised use, alteration, disclosure, distribution or review of this email is strictly prohibited. The information contained in this email, including any attachment sent with it, may be subject to a statutory duty of confidentiality if it relates to health service matters. If you are not the intended recipient(s), or if you have received this email in error, you are asked to immediately notify the sender by telephone collect on Australia +61 1800 198 175 or by return email. You should also delete this email, and any copies, from your computer system network and destroy any hard copies produced. If not an intended recipient of this email, you must not copy, distribute or take any action(s) that relies on it; any form of disclosure, modification, distribution and/or publication of this email is also prohibited. Although Queensland Health takes all reasonable steps to ensure this email does not contain malicious software, Queensland Health does not accept responsibility for the consequences if any person's computer inadvertently suffers any disruption to services, loss of information, harm or is infected with a virus, other malicious computer programme or code that may occur as a consequence of receiving this email. Unless stated otherwise, this email represents only the views of the sender and not the views of the Queensland Government. ** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _ This email has been scanned for the Sydney South West Area Health Service by the MessageLabs Email Security System. SSWAHS regularly monitors emails and attachments to ensure compliance with the NSW Government's Electronic Messaging Policy. This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please destroy it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of the Sydney South West Area Health Service. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu
