[Histonet] RE: flex kits
Lots of waste! Lee Ann Baldridge IUSM Indpls.,IN From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V [hor...@archildrens.org] Sent: Friday, August 21, 2009 3:49 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] flex kits If any of you are using the flex kits from Dako I would appreciate your feedback on what you think about them good and/or bad. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 1 Children's WaySlot 820 Little Rock, AR 72202 phone 501.364.4240 fax501.364.3155 visit us on the web at:www.archildrens.org ** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] PCP control
With the shortage of PCP controls what are you using as a possible alternative? Any information will be greatly appreciated. Thank you. Lin S. Bustamante, B.Sc.; HT(ASCP) Research Associate Histology Lab. Supervisor Dept. of Veterinary Integrative Biosciences Texas AM University College Station, TX 77843-4458 (979)845-3177 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] fat clearing reagent
Our home made recipe is : 1925 mls of 95% alcohol + 360 mls DH20 + 250 mls of Formaldehyde + 125 mls Glacial Acetic Acid. Got this 20 years ago from one of my pathologists' journals and it works really well. Let sit overnight and even the smallest nodes will turn opaque against a somewhat clear fatty background. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] reduction/elimination of red cell autofluorescence on FFPE sections for IF examination
Can anyone give feedback on reagents/procedures to use for immunofluorescence to reduce/eliminate red cell autofluorescence on FFPE sections. I am using an indirect method of labeling for two antibodies (FITC and Texas Red). I have not tried 0.1% sodium borohydride. Will this help for formalin fixed specimens? I have read that it is used for gluteraldehyde autofluorescence reduction. If appropriate how long should this reagent be applied to the specimen and at what temperature. This message (including any attachments) may contain confidential, proprietary, privileged and/or private information. The information is intended to be for the use of the individual or entity designated above. If you are not the intended recipient of this message, please notify the sender immediately, and delete the message and any attachments. Any disclosure, reproduction, distribution or other use of this message or any attachments by an individual or entity other than the intended recipient is prohibited. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] RE: reduction/elimination of red cell autofluorescence on FFPE sections for IF examination
We have been utilizing 0.25% Ammonium Hydroxide(28%) + 70% Ethanol v/v for 1 hour followed by 10 minutes in 50% Ethanol rinsing in several changes of buffer afterward and it seems to be working well for a multitude of autofluorescent problems on FFPE sections for us. We tried to Sodium Borohydride and it was a little diffiucult to work withquite hazardous. Amy S. Porter, HT (ASCP) QIHC Investigative HistoPathology Laboratory - Supervisor 2201 Biomedical Physical Sciences Bldg. Rm #2133 East Lansing, MI 48824-3320 Phone: (517) 884-5026 Fax: (517) 432-1368 Email: port...@msu.edu Web: www.humanpathology.msu.edu - Original Message - From: Thurby, Christina christina.thu...@bms.com To: Thurby, Christina christina.thu...@bms.com; histonet@lists.utsouthwestern.edu Sent: Monday, August 24, 2009 11:00 AM Subject: [Histonet] RE: reduction/elimination of red cell autofluorescence on FFPE sections for IF examination Sorry I did not include my contact information. Christina Thurby christina.thu...@bms.com 812-429-8097 Thanks! -Original Message- From: Thurby, Christina Sent: Monday, August 24, 2009 9:59 AM To: 'histonet@lists.utsouthwestern.edu' Subject: reduction/elimination of red cell autofluorescence on FFPE sections for IF examination Can anyone give feedback on reagents/procedures to use for immunofluorescence to reduce/eliminate red cell autofluorescence on FFPE sections. I am using an indirect method of labeling for two antibodies (FITC and Texas Red). I have not tried 0.1% sodium borohydride. Will this help for formalin fixed specimens? I have read that it is used for gluteraldehyde autofluorescence reduction. If appropriate how long should this reagent be applied to the specimen and at what temperature. This message (including any attachments) may contain confidential, proprietary, privileged and/or private information. The information is intended to be for the use of the individual or entity designated above. If you are not the intended recipient of this message, please notify the sender immediately, and delete the message and any attachments. Any disclosure, reproduction, distribution or other use of this message or any attachments by an individual or entity other than the intended recipient is prohibited. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Procedure for anti-human TCR V alpha24
Hello all, I am working in an Allergy and Immunology research lab and we have been trying to work up a flow cytometry antibody for IHC on paraffin. The antibody is mouse anti-human TCR V alpha24-Biotin from BeckmanCoulter. Does anyone have a working procedure for this antibody? I have tried many dilutions, retrieval methods and incubation times but nothing seems to work. Thanks in advance for your help. Sabina Sylvest CLS II Department of Allergy and Immunology Cincinnati Children's Research Foundation Cincinnati, OH (513)803-0741 ssylv...@cinci.rr.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Histotech Needed In Dover, FL Area
Good Morning/Afternoon Histonetters, Allied Search Partners is currently accepting resumes for our client in the Dover, FL area. We are prescreenign for the following: Position: Histotechnologists/Histotechnicians Shift:Full time/permanent, Day Shift Please submit resume for prescreening purposes to aly...@alliedsearchpartners.com *All inquiries are kept confidential* Be sure to visit our website to submit a job search request, refer a friend for $$Cash Bonus, and submit your resume to one of our career advisors. -- *Be sure to visit us on the web* www.alliedsearchpartners.com Alyssa Peterson, Director Of Recruitment Allied Search Partners O:888.388.7571 ext. 101 F: 888.388.7572 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] RE: reduction/elimination of red cell autofluorescence on FFPE sections for IF examination
The copper binds with the hemoglobin in the RBC and greatly reduces or eliminates autofluoresence depending on timing. 10 mM Copper Sulfate 10 mM Copper Sulfate Cupric Sulfate...1.25 gm 50 mM Amonium acetate (pH5)500.0 ml Adjust ph to 5.0 with 1.0 M NaOH 50 mM Ammonium acetate (pH5) Ammonium acetate.1.93 gm Distilled water500.0 ml Adjust pH to 5.0 with 1.0 M HCl 1. After staining wash slides in Reaction Buffer 2 times for 15 minutes each. 2. Rinse in PBS 2 times for 10 minutes each. 3. Rinse in distilled water 5 minutes. 4. Place slides in 10mM copper sulfate for 5 minutes. 5. Return slides to distilled water and check for autofluorescence with microscope. 6. if needed return slides to 10mM copper sulfate for a couple of more minutes and check again. 7. Rinse slides for 5 minutes in distilled water. 8. Rinse in PBS 2 times for 10 minutes each. 9. Coverslip slides with appropriate mounting media. James Watson HT ASCP Facilities Manager of Histology GNF Genomics Institute of the Novartis Research Foundation Tel 858-332-4647 Fax 858-812-1915 jwat...@gnf.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Amy Porter Sent: Monday, August 24, 2009 8:17 AM To: Thurby, Christina; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] RE: reduction/elimination of red cell autofluorescence on FFPE sections for IF examination We have been utilizing 0.25% Ammonium Hydroxide(28%) + 70% Ethanol v/v for 1 hour followed by 10 minutes in 50% Ethanol rinsing in several changes of buffer afterward and it seems to be working well for a multitude of autofluorescent problems on FFPE sections for us. We tried to Sodium Borohydride and it was a little diffiucult to work withquite hazardous. Amy S. Porter, HT (ASCP) QIHC Investigative HistoPathology Laboratory - Supervisor 2201 Biomedical Physical Sciences Bldg. Rm #2133 East Lansing, MI 48824-3320 Phone: (517) 884-5026 Fax: (517) 432-1368 Email: port...@msu.edu Web: www.humanpathology.msu.edu - Original Message - From: Thurby, Christina christina.thu...@bms.com To: Thurby, Christina christina.thu...@bms.com; histonet@lists.utsouthwestern.edu Sent: Monday, August 24, 2009 11:00 AM Subject: [Histonet] RE: reduction/elimination of red cell autofluorescence on FFPE sections for IF examination Sorry I did not include my contact information. Christina Thurby christina.thu...@bms.com 812-429-8097 Thanks! -Original Message- From: Thurby, Christina Sent: Monday, August 24, 2009 9:59 AM To: 'histonet@lists.utsouthwestern.edu' Subject: reduction/elimination of red cell autofluorescence on FFPE sections for IF examination Can anyone give feedback on reagents/procedures to use for immunofluorescence to reduce/eliminate red cell autofluorescence on FFPE sections. I am using an indirect method of labeling for two antibodies (FITC and Texas Red). I have not tried 0.1% sodium borohydride. Will this help for formalin fixed specimens? I have read that it is used for gluteraldehyde autofluorescence reduction. If appropriate how long should this reagent be applied to the specimen and at what temperature. This message (including any attachments) may contain confidential, proprietary, privileged and/or private information. The information is intended to be for the use of the individual or entity designated above. If you are not the intended recipient of this message, please notify the sender immediately, and delete the message and any attachments. Any disclosure, reproduction, distribution or other use of this message or any attachments by an individual or entity other than the intended recipient is prohibited. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] refrigerating sliver and gold chloride??
I wonder if 'storing these reagents in the refrigerator' concept originated with the idea of 'keeping them in the dark'. Janet From: histonet-boun...@lists.utsouthwestern.edu on behalf of John Kiernan Sent: Fri 8/21/2009 5:00 PM To: Cheri Miller Cc: histonet@lists.utsouthwestern.edu; histonet-boun...@lists.utsouthwestern.edu Subject: Re: [Histonet] refrigerating sliver and gold chloride?? There was never any reason to refrigerate gold chloride or silver nitrate. These compounds (solid or dissolved) can be kept for many years at room temperature. If the solutions are used repeatedly they eventually deteriorate from contamination with bits of sections, causing a changed appearance. Gold solutions take on a greenish grey hue and flakes of metallic gold eventually settle out. These can easily be recovered and recycled to make gold chloride (HAuCl4) again. Clean gold chloride solutions keep for ever. I have a few bottles of 0.5% that are still that beautiful yellow colour after about 25 years. Old silver nitrate looks a bit grey, not completely colourless. Re-purifying in a histology lab isn't really feasible. You can precipitate out and collect the silver, but (strangely) refining companies don't want it. Your message mentioned silver and gold chloride. I don't know a histological use for silver chloride, which is very sensitive to light - goes grey-violet as you look at it. John Kiernan Anatomy, UWO London, Canada = = = - Original Message - From: Cheri Miller cmil...@physlab.com Date: Friday, August 21, 2009 12:10 Subject: [Histonet] refrigerating sliver and gold chloride?? To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Cc: histonet-boun...@lists.utsouthwestern.edu histonet-boun...@lists.utsouthwestern.edu I just noticed that my silver and gold chloride says to store at room temp now. I have always stored them in the refrigerator. Is anyone still doing this?? Old habits die hard. I want to know if this has caused any staining issues for anyone. Cheryl Miller HT (ASCP) Histology Supervisor Physicians Laboratory,P.C. Omaha, Ne. 402 738 5052 PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet === The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. === ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] CD4 and CD8 in FFPE mouse tissue
Folks, It's my annual inquiry as to whether someone has optimized immunohistochemistry for CD4 and CD8 on formalin-fixed paraffin mouse tissue. Over the years we have tried lots of different antibodies but I am wondering if there is anything new that we have not tried. Any leads would be great! Thanks, Julie Julie Randolph-Habecker, Ph.D. Staff Scientist - Director Experimental Histopathology Shared Resource Fred Hutchinson Cancer Research Center 1100 Fairview Ave, N. DE-360 (Please note new location) Seattle WA 98109-1024 206-667-6119 jhabe...@fhcrc.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] water on slides?
When this happens in our lab, we put Drierite (anhydrous calcium sulfate) into the clearing agents. Just put enough to have not quite a solid layer on the bottom of the staining well, so it will not block the staining rack from going all the way into the clearing agent. We get ours from W.A. Hammond Drierite Company LTD, Xenia, Ohio 45385. Telephone number 937-376-2927. I hope this helps! Josie Britton HT Cheshire Medical Center Keene, NH 03431 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Hayes, Tina J. Sent: Monday, August 24, 2009 12:41 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] water on slides? We are having a problem with what seems to be water on our slides. We have had very high humidity levels in the air. It seems that we look at the slides before taking them to the pathology office, one pathologist reviews them and sees little or no water droplets, but as they sit and another pathologist reads them later, like the following day, the droplets are very apparent. We use Clearite-3. We have no xylene in our lab. We also coverslip with Permount. Is it possible that the clearite-3 and/or Permount is absorbing moisture from the atmosphere to the slides? And if so, do you have any suggestions for combating this issue? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This electronic message, including any attachments, is for the sole use of the intended recipients and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by electronic mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] water on slides?
We are having a very similar problem too. We change our alcohols and Histoclear like mad, but are having eosin bleeding out of the sections. I'm starting to wonder if it is the mounting media on our glass coverslipper. I ordered new bottle gaskets, so I guess I'll see what happens then. Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! Hayes, Tina J. tina.ha...@va.gov 8/24/2009 12:41 PM We are having a problem with what seems to be water on our slides. We have had very high humidity levels in the air. It seems that we look at the slides before taking them to the pathology office, one pathologist reviews them and sees little or no water droplets, but as they sit and another pathologist reads them later, like the following day, the droplets are very apparent. We use Clearite-3. We have no xylene in our lab. We also coverslip with Permount. Is it possible that the clearite-3 and/or Permount is absorbing moisture from the atmosphere to the slides? And if so, do you have any suggestions for combating this issue? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you.BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Bitting, Angela TEL;WORK:570-271-6844 ORG:;Histology EMAIL;WORK;PREF;NGW:akbitt...@geisinger.edu N:Bitting;Angela END:VCARD ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] IgG4
All, Is anyone performing paraffin block IHC staining for IgG4? If so, a vendor name would be much appreciated. Thank-you, Glen Dawson Milwaukee, WI ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Block Verification
Glen, We had the same situation about a year ago. We sent the block and a bucal smear out for DNA analysis. Now we have the Vantage system to assure positive patient id. Check into it. It saves time and worry and assures patient safety. Jan -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Dawson, Glen Sent: Monday, August 24, 2009 1:05 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Block Verification All, Our histology lab would like to verify that 2 paraffin blocks are from the same person to calm a pathologist's fears. Does anyone know of a good test for this purpose...PCR of some sort perhaps?? Any suggestions would be greatly appreciated. Thank-you In Advance, Glen Dawson BS, HT QIHC (ASCP) IHC Manager Milwaukee, WI ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel Health Systems, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Block Verification
Our Molecular Pathology Laboratory offers a multiplex PCR assay for identity markers for these situations. They receive specimens from all over the country for this testing (usually specimen mix-ups). It's not cheap and they only bill the referring facility. Richard Richard W. Cartun, Ph.D. Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax Dawson, Glen gdaw...@dynacaremilwaukee.com 8/24/2009 2:05 PM All, Our histology lab would like to verify that 2 paraffin blocks are from the same person to calm a pathologist's fears. Does anyone know of a good test for this purpose...PCR of some sort perhaps?? Any suggestions would be greatly appreciated. Thank-you In Advance, Glen Dawson BS, HT QIHC (ASCP) IHC Manager Milwaukee, WI ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Block Verification
You would send out for DNA fingerprinting. We have used the Cleveland Clinic for this. Christie From: janice.maho...@alegent.org To: gdaw...@dynacaremilwaukee.com; histonet@lists.utsouthwestern.edu Date: Mon, 24 Aug 2009 13:41:40 -0500 Subject: RE: [Histonet] Block Verification CC: Glen, We had the same situation about a year ago. We sent the block and a bucal smear out for DNA analysis. Now we have the Vantage system to assure positive patient id. Check into it. It saves time and worry and assures patient safety. Jan -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Dawson, Glen Sent: Monday, August 24, 2009 1:05 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Block Verification All, Our histology lab would like to verify that 2 paraffin blocks are from the same person to calm a pathologist's fears. Does anyone know of a good test for this purpose...PCR of some sort perhaps?? Any suggestions would be greatly appreciated. Thank-you In Advance, Glen Dawson BS, HT QIHC (ASCP) IHC Manager Milwaukee, WI ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel Health Systems, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] water on slides?
I'm getting the same eosin bleeding and water on the slides. I'm using Hemo-D and CitriSolv. I have changed the reagents and am still getting the eosin bleeding, the water on the slide is a new phenomenon. Any suggestions? Paula Sicurello VA Medical Center San Diego Veterans Medical Research Foundation (VMRF) Core for Micro Imaging(C-MI) 3350 La Jolla Village Dr., MC151 San Diego, CA 92161 858-552-8585 x2397 C-MI for your imaging needs. --- On Mon, 8/24/09, Angela Bitting akbitt...@geisinger.edu wrote: From: Angela Bitting akbitt...@geisinger.edu Subject: Re: [Histonet] water on slides? To: histonet@lists.utsouthwestern.edu, Tina J. Hayes tina.ha...@va.gov Date: Monday, August 24, 2009, 6:00 PM We are having a very similar problem too. We change our alcohols and Histoclear like mad, but are having eosin bleeding out of the sections. I'm starting to wonder if it is the mounting media on our glass coverslipper. I ordered new bottle gaskets, so I guess I'll see what happens then. Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! Hayes, Tina J. tina.ha...@va.gov 8/24/2009 12:41 PM We are having a problem with what seems to be water on our slides. We have had very high humidity levels in the air. It seems that we look at the slides before taking them to the pathology office, one pathologist reviews them and sees little or no water droplets, but as they sit and another pathologist reads them later, like the following day, the droplets are very apparent. We use Clearite-3. We have no xylene in our lab. We also coverslip with Permount. Is it possible that the clearite-3 and/or Permount is absorbing moisture from the atmosphere to the slides? And if so, do you have any suggestions for combating this issue? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. -Inline Attachment Follows- ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] water on slides?
If your solutions are clean the cause may be that the level of the water wash station on your stainer is higher than the level of the subsequent alcohol container which means that some water will not be removed from the top of the slide. This is common with running water washes on stainers as the water pressure will elevate the level. A common artifact appears as a blue stripe down the slide caused by the water running down and removing the eosin. regards Tony Reilly Chief Scientist Anatomical Pathology Pathology Queensland Level 1, Building 15 Princess Alexandra Hospital Ipswich Rd, Woolloongabba Q 4102 Australia Ph: 07 32402412 Fax:07 32402930 tony_rei...@health.qld.gov.au Va Paula Sicurello vapat...@yahoo.com 25/08/2009 7:30 am I'm getting the same eosin bleeding and water on the slides. I'm using Hemo-D and CitriSolv. I have changed the reagents and am still getting the eosin bleeding, the water on the slide is a new phenomenon. Any suggestions? Paula Sicurello VA Medical Center San Diego Veterans Medical Research Foundation (VMRF) Core for Micro Imaging(C-MI) 3350 La Jolla Village Dr., MC151 San Diego, CA 92161 858-552-8585 x2397 C-MI for your imaging needs. --- On Mon, 8/24/09, Angela Bitting akbitt...@geisinger.edu wrote: From: Angela Bitting akbitt...@geisinger.edu Subject: Re: [Histonet] water on slides? To: histonet@lists.utsouthwestern.edu, Tina J. Hayes tina.ha...@va.gov Date: Monday, August 24, 2009, 6:00 PM We are having a very similar problem too. We change our alcohols and Histoclear like mad, but are having eosin bleeding out of the sections. I'm starting to wonder if it is the mounting media on our glass coverslipper. I ordered new bottle gaskets, so I guess I'll see what happens then. Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! Hayes, Tina J. tina.ha...@va.gov 8/24/2009 12:41 PM We are having a problem with what seems to be water on our slides. We have had very high humidity levels in the air. It seems that we look at the slides before taking them to the pathology office, one pathologist reviews them and sees little or no water droplets, but as they sit and another pathologist reads them later, like the following day, the droplets are very apparent. We use Clearite-3. We have no xylene in our lab. We also coverslip with Permount. Is it possible that the clearite-3 and/or Permount is absorbing moisture from the atmosphere to the slides? And if so, do you have any suggestions for combating this issue? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. -Inline Attachment Follows- ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This email, including any attachments sent with it, is confidential and for the sole use of the intended recipient(s). This confidentiality is not waived or lost, if you receive it and you are not the intended recipient(s), or if it is transmitted/received in error. Any unauthorised use, alteration, disclosure, distribution or review of this email is strictly prohibited. The information contained in this email, including any attachment sent with it, may be subject to a statutory duty of confidentiality if it relates to health service matters. If you are not the intended recipient(s), or if you have received this email in error, you are asked to immediately notify the sender by telephone collect on Australia +61 1800 198 175 or by return email. You should also delete this email, and any copies, from your computer system network and destroy any hard copies produced. If not an
