RE: [Histonet] Preventing slide labeling mistakes
You must match your blocks and slides. This will eliminate a lot of your mistakes/ NOT ALL! Mike -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lynette Pavelich Sent: Thursday, November 19, 2009 9:52 PM To: histonet@lists.utsouthwestern.edu; timothy.mor...@ucsfmedctr.org Subject: Re: [Histonet] Preventing slide labeling mistakes Hi Tim, We do everything you do, but we also match our blocks to the written number on the slides. We catch the majority of our mistakes at that point. The other is because the font on the label is so small, we have a hard time seeing the difference between 5's 6's!!! I'm sure it's not our ages or anything! We're working with CoPath to get a larger font!! Ahhh, getting old is SO fun! And yes, reading glasses work pretty good!! LOL Have a great weekend everyone!! Lynette Lynette Pavelich, HT(ASCP) Histology Supervisor MSH Competency Coordinator Hurley Medical Center One Hurley Plaza Flint, MI 48503 email: lpave...@hurleymc.com ph: 810-257-9948 fax: 810-762-7082 Morken, Tim timothy.mor...@ucsfmedctr.org 11/19/09 7:20 PM Hi, Can people share their procedures for preventing manual slide labeling mistakes? No need to include barcoding - we are exploring that but it is a ways off. We currently have a manual process: We prohibit pre-labeling slides in batches (many blocks/slides at one time), require labeling slides (hand-written) only at the time of cutting a single block, and matching paper label to the slide after staining. We don't currently match blocks to slides. Thanks for any info! Tim Morken Supervisor, Histology / IPOX UCSF Medical Center San Francisco, CA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Preventing slide labeling mistakes
Lynette, You might want to also investigate different fonts if that is an option. Keeping the same size but using a different font can make a world of difference. Victor Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 vic...@pathology.washington.edu 206-598-2792 206-598-7659 Fax = Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. Lynette Pavelich wrote: Hi Tim, We do everything you do, but we also match our blocks to the written number on the slides. We catch the majority of our mistakes at that point. The other is because the font on the label is so small, we have a hard time seeing the difference between 5's 6's!!! I'm sure it's not our ages or anything! We're working with CoPath to get a larger font!! Ahhh, getting old is SO fun! And yes, reading glasses work pretty good!! LOL Have a great weekend everyone!! Lynette Lynette Pavelich, HT(ASCP) Histology Supervisor MSH Competency Coordinator Hurley Medical Center One Hurley Plaza Flint, MI 48503 email: lpave...@hurleymc.com ph: 810-257-9948 fax: 810-762-7082 Morken, Tim timothy.mor...@ucsfmedctr.org 11/19/09 7:20 PM Hi, Can people share their procedures for preventing manual slide labeling mistakes? No need to include barcoding - we are exploring that but it is a ways off. We currently have a manual process: We prohibit pre-labeling slides in batches (many blocks/slides at one time), require labeling slides (hand-written) only at the time of cutting a single block, and matching paper label to the slide after staining. We don't currently match blocks to slides. Thanks for any info! Tim Morken Supervisor, Histology / IPOX UCSF Medical Center San Francisco, CA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Preventing slide labeling mistakes
Thanks for the suggestions. One clarification: matching blocks and slides CATCHES mistakes; it does not reduce or eliminate them. Tim -Original Message- From: Mike Pence [mailto:mpe...@grhs.net] Sent: Friday, November 20, 2009 6:38 AM To: Lynette Pavelich; histonet@lists.utsouthwestern.edu; Morken, Tim Subject: RE: [Histonet] Preventing slide labeling mistakes You must match your blocks and slides. This will eliminate a lot of your mistakes/ NOT ALL! Mike -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lynette Pavelich Sent: Thursday, November 19, 2009 9:52 PM To: histonet@lists.utsouthwestern.edu; timothy.mor...@ucsfmedctr.org Subject: Re: [Histonet] Preventing slide labeling mistakes Hi Tim, We do everything you do, but we also match our blocks to the written number on the slides. We catch the majority of our mistakes at that point. The other is because the font on the label is so small, we have a hard time seeing the difference between 5's 6's!!! I'm sure it's not our ages or anything! We're working with CoPath to get a larger font!! Ahhh, getting old is SO fun! And yes, reading glasses work pretty good!! LOL Have a great weekend everyone!! Lynette Lynette Pavelich, HT(ASCP) Histology Supervisor MSH Competency Coordinator Hurley Medical Center One Hurley Plaza Flint, MI 48503 email: lpave...@hurleymc.com ph: 810-257-9948 fax: 810-762-7082 Morken, Tim timothy.mor...@ucsfmedctr.org 11/19/09 7:20 PM Hi, Can people share their procedures for preventing manual slide labeling mistakes? No need to include barcoding - we are exploring that but it is a ways off. We currently have a manual process: We prohibit pre-labeling slides in batches (many blocks/slides at one time), require labeling slides (hand-written) only at the time of cutting a single block, and matching paper label to the slide after staining. We don't currently match blocks to slides. Thanks for any info! Tim Morken Supervisor, Histology / IPOX UCSF Medical Center San Francisco, CA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] CAP guidelines for positive controls
Hello, I am trying to find the guidelines for CAP that describes how long IHC positive control slides are to be kept on file ... does anyone know? Thanks in advance! Tiana This email and/or any documents in this transmission is intended for the addressee(s) only and may contain legally privileged or confidential information. Any unauthorized use, disclosure, distribution, copying or dissemination is strictly prohibited. If you receive this transmission in error, please notify the sender immediately and return the original. Ce courriel et tout document dans cette transmission est destiné à la personne ou aux personnes à qui il est adressé. Il peut contenir des informations privilégiées ou confidentielles. Toute utilisation, divulgation, distribution, copie, ou diffusion non autorisée est strictement défendue. Si vous n'êtes pas le destinataire de ce message, veuillez en informer l'expéditeur immédiatement et lui remettre l'original. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] CAP QUESTION ANP.12425 ASR disclaimer on pathology reports
What is the current practice for meeting this regulation? Is a general statement (disclaimer) placed on all pathology reports that have immunocytochemistry and/or FISH and ISH or is the disclaimer placed only on the reports where the class 1 ASR antibody or nucleic acid have been used? Thanks for your input? Annette Foshey Charge Tech in Histology Children's Hospital of Wisconsin 414-266-6580Fax 414-266-2779 afos...@chw.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] course recommendation
I am looking for a conference/course to attend in 2010. I am experienced in IF and IHC and would like to learn more about any new techniques and products that might be available. Does anyone have a recommendation for a course or a conference in the US? I am not a certified HT but I pretend to be one:) Thanks for any suggestions!! Mike ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] CAP QUESTION ANP.12425 ASR disclaimer on pathology reports
We used to add that the methods were experimental. René J. --- On Fri, 11/20/09, Foshey, Annette afos...@chw.org wrote: From: Foshey, Annette afos...@chw.org Subject: [Histonet] CAP QUESTION ANP.12425 ASR disclaimer on pathology reports To: (histonet@lists.utsouthwestern.edu) histonet@lists.utsouthwestern.edu Date: Friday, November 20, 2009, 12:54 PM What is the current practice for meeting this regulation? Is a general statement (disclaimer) placed on all pathology reports that have immunocytochemistry and/or FISH and ISH or is the disclaimer placed only on the reports where the class 1 ASR antibody or nucleic acid have been used? Thanks for your input? Annette Foshey Charge Tech in Histology Children's Hospital of Wisconsin 414-266-6580 Fax 414-266-2779 afos...@chw.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
AW: [Histonet] Preventing slide labeling mistakes
We write a macro-protocol at the time of grossing with all blocknumbers and additional identifer. This paper is matched the first time with all blocks after embedding and before cutting. Missing blocks or wrong labelled blocks are found. The second time the paper is matched with all slides after staining and before delivering. Missing and wrong labelled slides are found. Additional the slide-number and material is matched with the case sheet. The slides are labelled batchwise with 10 per batch immediatly before cutting. The identifiers (letters, lateral, resection-margin, etc.) are written on side of the cassette, so numbers can be written big enough. The numbers have maximum of 5 digits slash year. 12345/09 Gudrun -Ursprüngliche Nachricht- Von: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von Morken, Tim Gesendet: Freitag, 20. November 2009 01:20 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Preventing slide labeling mistakes Hi, Can people share their procedures for preventing manual slide labeling mistakes? No need to include barcoding - we are exploring that but it is a ways off. We currently have a manual process: We prohibit pre-labeling slides in batches (many blocks/slides at one time), require labeling slides (hand-written) only at the time of cutting a single block, and matching paper label to the slide after staining. We don't currently match blocks to slides. Thanks for any info! Tim Morken Supervisor, Histology / IPOX UCSF Medical Center San Francisco, CA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] CAP QUESTION ANP.12425 ASR disclaimer on pathologyreports
We add ours to only the Class I ASR cases. Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Foshey, Annette Sent: Friday, November 20, 2009 12:55 To: (histonet@lists.utsouthwestern.edu) Subject: [Histonet] CAP QUESTION ANP.12425 ASR disclaimer on pathologyreports What is the current practice for meeting this regulation? Is a general statement (disclaimer) placed on all pathology reports that have immunocytochemistry and/or FISH and ISH or is the disclaimer placed only on the reports where the class 1 ASR antibody or nucleic acid have been used? Thanks for your input? Annette Foshey Charge Tech in Histology Children's Hospital of Wisconsin 414-266-6580Fax 414-266-2779 afos...@chw.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] CAP guidelines for positive controls
They should be kept to comply with your policies and the state requirements. Remember that CAP has regulations but cannot override state nor institutional policies. René J. --- On Fri, 11/20/09, Tiana Fountain tfount...@exchange.hsc.mb.ca wrote: From: Tiana Fountain tfount...@exchange.hsc.mb.ca Subject: [Histonet] CAP guidelines for positive controls To: histonet@lists.utsouthwestern.edu Date: Friday, November 20, 2009, 12:14 PM Hello, I am trying to find the guidelines for CAP that describes how long IHC positive control slides are to be kept on file ... does anyone know? Thanks in advance! Tiana -Inline Attachment Follows- This email and/or any documents in this transmission is intended for the addressee(s) only and may contain legally privileged or confidential information. Any unauthorized use, disclosure, distribution, copying or dissemination is strictly prohibited. If you receive this transmission in error, please notify the sender immediately and return the original. Ce courriel et tout document dans cette transmission est destiné à la personne ou aux personnes à qui il est adressé. Il peut contenir des informations privilégiées ou confidentielles. Toute utilisation, divulgation, distribution, copie, ou diffusion non autorisée est strictement défendue. Si vous n'êtes pas le destinataire de ce message, veuillez en informer l'expéditeur immédiatement et lui remettre l'original. -Inline Attachment Follows- ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] course recommendation
Take a look at the National Society for Histotechnology (www.nsh.org). There are several State and Regional Society meetings coming up in the spring of 2010 and then of course there is the National Symposium/Convention in the Fall of 2010 in Seattle, WA. Jack Ratliff NSH -Hard Tissue Committee Chair Date: Fri, 20 Nov 2009 12:57:36 -0500 From: mti...@trudeauinstitute.org To: histonet@lists.utsouthwestern.edu Subject: [Histonet] course recommendation I am looking for a conference/course to attend in 2010. I am experienced in IF and IHC and would like to learn more about any new techniques and products that might be available. Does anyone have a recommendation for a course or a conference in the US? I am not a certified HT but I pretend to be one:) Thanks for any suggestions!! Mike ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Preventing slide labeling mistakes
Linda, Yes, I should have said matching blocks and slides after staining catches mistakes but does not eliminate them. We are looking at: isolating block to be cut, labeling slide just before or after sectioning, matching block to slide after sectioning at the cutting station. Unfortunately with a manual method it comes to taking a lot of care with what one does up front. No amount of error checking will catch all possible mistakes, and can actually lead to more if it is too tedious and tiring. Tim -Original Message- From: Sebree Linda A [mailto:lseb...@uwhealth.org] Sent: Friday, November 20, 2009 8:55 AM To: Morken, Tim; Mike Pence; Lynette Pavelich; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Preventing slide labeling mistakes If the matching is done at the time of sectioning, i.e. making sure the block you are about to cut matches the slide you have ready to pick up that block's section(s), it will reduce mislabeling errorsand its GLP! Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Morken, Tim Sent: Friday, November 20, 2009 10:42 AM To: Mike Pence; Lynette Pavelich; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Preventing slide labeling mistakes Thanks for the suggestions. One clarification: matching blocks and slides CATCHES mistakes; it does not reduce or eliminate them. Tim -Original Message- From: Mike Pence [mailto:mpe...@grhs.net] Sent: Friday, November 20, 2009 6:38 AM To: Lynette Pavelich; histonet@lists.utsouthwestern.edu; Morken, Tim Subject: RE: [Histonet] Preventing slide labeling mistakes You must match your blocks and slides. This will eliminate a lot of your mistakes/ NOT ALL! Mike -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lynette Pavelich Sent: Thursday, November 19, 2009 9:52 PM To: histonet@lists.utsouthwestern.edu; timothy.mor...@ucsfmedctr.org Subject: Re: [Histonet] Preventing slide labeling mistakes Hi Tim, We do everything you do, but we also match our blocks to the written number on the slides. We catch the majority of our mistakes at that point. The other is because the font on the label is so small, we have a hard time seeing the difference between 5's 6's!!! I'm sure it's not our ages or anything! We're working with CoPath to get a larger font!! Ahhh, getting old is SO fun! And yes, reading glasses work pretty good!! LOL Have a great weekend everyone!! Lynette Lynette Pavelich, HT(ASCP) Histology Supervisor MSH Competency Coordinator Hurley Medical Center One Hurley Plaza Flint, MI 48503 email: lpave...@hurleymc.com ph: 810-257-9948 fax: 810-762-7082 Morken, Tim timothy.mor...@ucsfmedctr.org 11/19/09 7:20 PM Hi, Can people share their procedures for preventing manual slide labeling mistakes? No need to include barcoding - we are exploring that but it is a ways off. We currently have a manual process: We prohibit pre-labeling slides in batches (many blocks/slides at one time), require labeling slides (hand-written) only at the time of cutting a single block, and matching paper label to the slide after staining. We don't currently match blocks to slides. Thanks for any info! Tim Morken Supervisor, Histology / IPOX UCSF Medical Center San Francisco, CA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] frozen sections not adhering to slide
I am processing adult rat brain sections for routine histological stains, but am having a terrible time getting them to stick to the slide. After transcardial perfusion the rat brains are dissected out and processed for frozen sectioning. I post-fix overnight in PFA, cryoprotect in 30%sucrose until the brains sink and flash freeze in isopentane. All seems like pretty standard stuff. After cutting 45 um thick sections and storing them in antifreeze solution (30% 0.1M Phosphate Buffer, 30% Glycerol, 40% Ethylene Glycol), I mount them on slides. After letting them dry overnight, I rehydrate/wash in 0.1M Phosphate Buffer. Frequently, the sections start to wrinkle up and start coming of the slide during this first step. I'm using superfrost+ slides from fisher. Does anyone have any advice? Please help. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: SPAM-LOW: [Histonet] course recommendation
Check out this Applied Immunohistochemistry and Molecular Pathology (AIMP) meeting coming at end of January 2009 in Florida. the fourth annual international retreat on applied IHC and molecular pathology. Please visit http://www.pathlearning.com/Pathology_Learning_Centers/Welcome.html for detailed information of the program and how to register. Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mike Tighe Sent: Friday, November 20, 2009 10:58 AM To: histonet@lists.utsouthwestern.edu Subject: SPAM-LOW: [Histonet] course recommendation I am looking for a conference/course to attend in 2010. I am experienced in IF and IHC and would like to learn more about any new techniques and products that might be available. Does anyone have a recommendation for a course or a conference in the US? I am not a certified HT but I pretend to be one:) Thanks for any suggestions!! Mike ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] CAP QUESTION ANP.12425 ASR disclaimer on pathologyreports
We use the statement for all reports that any IHC/ISH, regardless if they are ASRs or not. Martha Ward Wake Forest University Baptist Medical Center -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Foshey, Annette Sent: Friday, November 20, 2009 12:55 PM To: (histonet@lists.utsouthwestern.edu) Subject: [Histonet] CAP QUESTION ANP.12425 ASR disclaimer on pathologyreports What is the current practice for meeting this regulation? Is a general statement (disclaimer) placed on all pathology reports that have immunocytochemistry and/or FISH and ISH or is the disclaimer placed only on the reports where the class 1 ASR antibody or nucleic acid have been used? Thanks for your input? Annette Foshey Charge Tech in Histology Children's Hospital of Wisconsin 414-266-6580Fax 414-266-2779 afos...@chw.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] frozen sections not adhering to slide
I am processing adult rat brain sections for routine histological stains, but am having a terrible time getting them to stick to the slide. After transcardial perfusion the rat brains are dissected out and processed for frozen sectioning. I post-fix overnight in PFA, cryoprotect in 30%sucrose until the brains sink and flash freeze in isopentane. All seems like pretty standard stuff. After cutting 45 um thick sections and storing them in antifreeze solution (30% 0.1M Phosphate Buffer, 30% Glycerol, 40% Ethylene Glycol), I mount them on slides. After letting them dry overnight, I rehydrate/wash in 0.1M Phosphate Buffer. Frequently, the sections start to wrinkle up and start coming of the slide during this first step. I'm using superfrost+ slides from fisher. Does anyone have any advice? Please help. Weight Loss Program Best Weight Loss Program - Click Here! http://tagline.iwon.com/c?cp=DNzWnQGT6RwmX-EIosC0AQAAKZwW4ZcSHBdS1PQuiZmGMQTOAAYAAADNAAAEUlAqW_0= ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] CAP QUESTION ANP.12425 ASR disclaimer on pathology reports
The disclaimer is only for ASR antibodies. They don't have to be labeled experimental because a CLIA certified lab has full capability and authority to validate any antibody they want to use for any purpose. You do have to document your validation procedure and results. You can also use RUO antibodies under CLIA regs as long as you do the full documented validation. Interestingly there is no suggested disclaimer for RUO antibodies. Tim Morken Supervisor, Histology / IPOX UCSF Medical Center San Francisco, CA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Foshey, Annette Sent: Friday, November 20, 2009 9:55 AM To: (histonet@lists.utsouthwestern.edu) Subject: [Histonet] CAP QUESTION ANP.12425 ASR disclaimer on pathology reports What is the current practice for meeting this regulation? Is a general statement (disclaimer) placed on all pathology reports that have immunocytochemistry and/or FISH and ISH or is the disclaimer placed only on the reports where the class 1 ASR antibody or nucleic acid have been used? Thanks for your input? Annette Foshey Charge Tech in Histology Children's Hospital of Wisconsin 414-266-6580Fax 414-266-2779 afos...@chw.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] CAP QUESTION ANP.12425 ASR disclaimer onpathology reports
Just can't bill for RUO, correct? -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Morken, Tim Sent: Friday, November 20, 2009 13:24 To: Foshey, Annette; (histonet@lists.utsouthwestern.edu) Subject: RE: [Histonet] CAP QUESTION ANP.12425 ASR disclaimer onpathology reports The disclaimer is only for ASR antibodies. They don't have to be labeled experimental because a CLIA certified lab has full capability and authority to validate any antibody they want to use for any purpose. You do have to document your validation procedure and results. You can also use RUO antibodies under CLIA regs as long as you do the full documented validation. Interestingly there is no suggested disclaimer for RUO antibodies. Tim Morken Supervisor, Histology / IPOX UCSF Medical Center San Francisco, CA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Foshey, Annette Sent: Friday, November 20, 2009 9:55 AM To: (histonet@lists.utsouthwestern.edu) Subject: [Histonet] CAP QUESTION ANP.12425 ASR disclaimer on pathology reports What is the current practice for meeting this regulation? Is a general statement (disclaimer) placed on all pathology reports that have immunocytochemistry and/or FISH and ISH or is the disclaimer placed only on the reports where the class 1 ASR antibody or nucleic acid have been used? Thanks for your input? Annette Foshey Charge Tech in Histology Children's Hospital of Wisconsin 414-266-6580Fax 414-266-2779 afos...@chw.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] NYState licensing examination
Dear Histonetters, I am collecting any hand-outs, pamphlets, archives, books, CD's, anything you may have extra or may be willing to share that can help me prepare for the New York State Histologic Technician Examination. The examination may be similar to the HT and HTL exam; so anything related to these examinations will help. Thank you all in advance, Milton Milton A. Gomez, HTL (ASCP) Technical Supervisor Immunohistochemistry Department ARUP Laboratories, Inc. 500 Chipeta Way Salt Lake City, UT 84108-1221 Desk Phone: 801-583-2787, ext.3869 Lab. Phone: 801-584-5257/5242 Fax: 801-584-5217 E-mail: milton.go...@aruplab.com mailto:milton.go...@aruplab.com Web: www.aruplab.com http://www.aruplab.com/ - -- The information transmitted by this e-mail and any included attachments are from ARUP Laboratories and are intended only for the recipient. The information contained in this message is confidential and may constitute inside or non-public information under international, federal, or state securities laws, or protected health information and is intended only for the use of the recipient. Unauthorized forwarding, printing, copying, distributing, or use of such information is strictly prohibited and may be unlawful. If you are not the intended recipient, please promptly delete this e-mail and notify the sender of the delivery error or you may call ARUP Laboratories Compliance Hot Line in Salt Lake City, Utah USA at (+1 (800) 522-2787 ext. 2100 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] ISH
Dear Histonetters, I have a problem with my ISH and maybe one of you can help me. I used probes I *know* they are working. I put the mice embryos (E 10.5) in BM Purple for staining. Unfortunately they turned black in a short time. They were not stained in the inside. It looks like that the staining precipitated on the surface and no reaction did start in the inside. A few weeks ago I did an ISH to ( same tissue) and put the embryos in BM Purple over night in the fridge, because normally the staining should stop. It did not ! Does anybody know something about this problem? Thanks in advance, Sandra Moeller ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Lumpectomy question
Good Friday Afternoon, Does anyone know if there is a modifier for 88307 for Lumpectomies when 20-30 blocks are submitted. We are currently charging 88307 but the pathologist thinks there should be a modifier. Thanks. Debbie M. Boyd, HT(ASCP) I Chief Histologist I Southside Regional Medical Center I 200 Medical Park Boulevard I Petersburg, Va. 23805 I T: 804-765-5050 I F: 804-765-5582 I dkb...@chs.net -- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] CMS/NCCI Update Dated October 1, 2009
According the one of our pathologist the above mentioned update clarified that CMS allows the billing of special stains performed on different blocks of the same Pathology specimen. Previously, it was understood that a special stain could only be billed once per specimen. Now it appears that a stain could be billed twice if performed on block 1 and block 3 of the same specimen. Has anyone else reached this conclusion? Thanks, Joyce Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] CMS/NCCI Update Dated October 1, 2009
Joyce, That's very interesting, as I remember going to great lengths a few years ago to be sure we had a billing limiter in place in CoPath to prevent the multiple charging of CPT codes on the same specimen. However, I looked up the document released on 10/1/09 and here's what I found in regards to the special and immuno CPT codes: 8. The unit of service for special stains (CPT codes 88312-88313) and immunohistochemistry (CPT codes 88342, 88360, 88361) is each stain. If it is medically reasonable and necessary to perform the same stain on more than one specimen or more than one block of tissue from the same specimen, additional units of service may be reported for the additional specimen(s) or block(s). Physicians should not report more than one unit of service for a stain performed on a single tissue block. For example it is common practice to cut multiple levels from a tissue block and stain each level with the same stain. The multiple levels from the same block of tissue stained with the same stain should not be reported as additional units of service. Only one unit of service should be reported for the stain on multiple levels from the single tissue block. Additionally, controls performed with special stains should not be reported as separate units of service for the stain. From what all I can see, you're right; it seems that we can bill for multiple special stains on the same specimen, just not the same block (so tell Drs. Stargel and Sears it won't help them :) ). I'm going to present this to our pathologists and billing auditors for review. Thanks so much for sharing this with everyone!!! Drew On Fri, Nov 20, 2009 at 14:28, Weems, Joyce jwe...@sjha.org wrote: According the one of our pathologist the above mentioned update clarified that CMS allows the billing of special stains performed on different blocks of the same Pathology specimen. Previously, it was understood that a special stain could only be billed once per specimen. Now it appears that a stain could be billed twice if performed on block 1 and block 3 of the same specimen. Has anyone else reached this conclusion? Thanks, Joyce Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] anti-human Notch4
Hi friends, My PI is asking for Notch 4 IHC on FFPE tissue. Can any of you suggest me a good Ab (preferably mot mouse but anti-human Notch4) and protocol please! Thanks in advance, Naira Naira V. Margaryan, D.V.M., Ph.D. Research Scientist Children's Memorial Research Center 2300 Children's Plaza, Box 222 Chicago, IL 60614-3363 Tel: 773-755-6340 Fax: 773-755-6594 nmargar...@childrensmemorial.orgmailto:nmargar...@childrensmemorial.org For Express Mail: CMRC, Room C.473 2430 N. Halsted Street Chicago, IL 60614-4314 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] anti-human Notch4
I am interested in the same antibody. Thanks, Beatrice DeBrosse-Serra Pathology Scientist Pfizer Global Research Development CB4, 2150 10646 Science Center Drive San Diego, CA 92121 Phone# 858-622-5986 Fax# 858-678-8290 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Margaryan, Naira Sent: Friday, November 20, 2009 12:38 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] anti-human Notch4 Hi friends, My PI is asking for Notch 4 IHC on FFPE tissue. Can any of you suggest me a good Ab (preferably mot mouse but anti-human Notch4) and protocol please! Thanks in advance, Naira Naira V. Margaryan, D.V.M., Ph.D. Research Scientist Children's Memorial Research Center 2300 Children's Plaza, Box 222 Chicago, IL 60614-3363 Tel: 773-755-6340 Fax: 773-755-6594 nmargar...@childrensmemorial.orgmailto:nmargar...@childrensmemorial.org For Express Mail: CMRC, Room C.473 2430 N. Halsted Street Chicago, IL 60614-4314 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Lumpectomy question
Debbie, as far as I know, there is no modifier, all you can charge is a 88307. That's the same case when you have a skin excision at a 88305. Even if it's a melanoma excision and it takes 20 blocks, you can only charge an 88305. The system is FAR from perfect. JTT - Original Message - From: dkb...@chs.net To: histonet@lists.utsouthwestern.edu Sent: Friday, November 20, 2009 1:17 PM Subject: [Histonet] Lumpectomy question Good Friday Afternoon, Does anyone know if there is a modifier for 88307 for Lumpectomies when 20-30 blocks are submitted. We are currently charging 88307 but the pathologist thinks there should be a modifier. Thanks. Debbie M. Boyd, HT(ASCP) I Chief Histologist I Southside Regional Medical Center I 200 Medical Park Boulevard I Petersburg, Va. 23805 I T: 804-765-5050 I F: 804-765-5582 I dkb...@chs.net -- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] CAP QUESTION ANP.12425 ASR disclaimer onpathology reports
I have a question similar to this one. If I take 2 IVD antibodies say cytokeratin AE1/AE3 and 34BF12 and make these into one cytokeratin cocktail, is this considered an ASR because I combined them into another antibody? Joe - Original Message - From: Morken, Tim timothy.mor...@ucsfmedctr.org To: Foshey, Annette afos...@chw.org; histonet@lists.utsouthwestern.edu Sent: Friday, November 20, 2009 12:24 PM Subject: RE: [Histonet] CAP QUESTION ANP.12425 ASR disclaimer onpathology reports The disclaimer is only for ASR antibodies. They don't have to be labeled experimental because a CLIA certified lab has full capability and authority to validate any antibody they want to use for any purpose. You do have to document your validation procedure and results. You can also use RUO antibodies under CLIA regs as long as you do the full documented validation. Interestingly there is no suggested disclaimer for RUO antibodies. Tim Morken Supervisor, Histology / IPOX UCSF Medical Center San Francisco, CA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Foshey, Annette Sent: Friday, November 20, 2009 9:55 AM To: (histonet@lists.utsouthwestern.edu) Subject: [Histonet] CAP QUESTION ANP.12425 ASR disclaimer on pathology reports What is the current practice for meeting this regulation? Is a general statement (disclaimer) placed on all pathology reports that have immunocytochemistry and/or FISH and ISH or is the disclaimer placed only on the reports where the class 1 ASR antibody or nucleic acid have been used? Thanks for your input? Annette Foshey Charge Tech in Histology Children's Hospital of Wisconsin 414-266-6580Fax 414-266-2779 afos...@chw.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] rhodanin stain fading
Alcohol will cause the stain to fade, even the small amt. that is carried over to the xylene over the course of the day. Make sure your xylene is fresh and coverslip immediately after staining Rena Fail - Original Message From: Gudrun Lang gu.l...@gmx.at To: histonet@lists.utsouthwestern.edu Sent: Fri, November 20, 2009 2:16:49 PM Subject: [Histonet] rhodanin stain fading Hi! Yesterday I did a rhodine stain for copper. Immediatly after staining I saw not many but distinct red granula in hepatocytes. I am new to this stain, so I was happy (and a little bit proud), that it had worked. I also stained one slide over night for comparison. Today morning the first stained slides has faded and not the smallest bit of red colour could be seen. Is this a common problem? What causes the fast fading? Gudrun ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
AW: [Histonet] rhodanin stain fading
Rena, we use butylacetat for clearing and before coverslipping with Pertex (xylolbased, resinous). Butylacetat tolerates ethanol-carryover more than xylene, but evapourates very fast after couverslipping. But is it possible, that butylacetat or Pertex itself cause the fading? Do you think coverslipping with a waterbased medium is a good alternative? Gudrun -Ursprüngliche Nachricht- Von: Rena Fail [mailto:renaf...@bellsouth.net] Gesendet: Samstag, 21. November 2009 00:15 An: gu.l...@gmx.at; histonet@lists.utsouthwestern.edu Betreff: Re: [Histonet] rhodanin stain fading Alcohol will cause the stain to fade, even the small amt. that is carried over to the xylene over the course of the day. Make sure your xylene is fresh and coverslip immediately after staining Rena Fail - Original Message From: Gudrun Lang gu.l...@gmx.at To: histonet@lists.utsouthwestern.edu Sent: Fri, November 20, 2009 2:16:49 PM Subject: [Histonet] rhodanin stain fading Hi! Yesterday I did a rhodine stain for copper. Immediatly after staining I saw not many but distinct red granula in hepatocytes. I am new to this stain, so I was happy (and a little bit proud), that it had worked. I also stained one slide over night for comparison. Today morning the first stained slides has faded and not the smallest bit of red colour could be seen. Is this a common problem? What causes the fast fading? Gudrun ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
