[Histonet] Re: bone marrow fixative
Now that we can no longer use mercury-containing fixatives, I don't think that the various proprietary fixatives advocated for bone marrow add anything to the morphology, and some of them can gum up processors or interfere with immunohistochemistry. Neutral buffered formalin requires time for fixation - clots need to be cut up as soon as possible after they're received, and biopsy specimens really ought to fix overnight before decalcification and processing. Communication with oncologists is essential (and rarely achievable). Bob Richmond Samurai Pathologist Knoxville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Deleting extraneous parts of posts when replying
My brief experience with paraffin and fluorescence (DAPI and EGFP) is that the paraffin embedding process destroys it. Regards, Merced --On Tuesday, March 02, 2010 3:25 PM -0600 Fabrice gankam gan...@googlemail.com wrote: Hi, Just wondering if anyone has use hydroethinide to detect the free radicals (ROS) in the CNS or anyother tissue of rats after 4% formallin fixation and parrafin embedding. I wanted to used the hydroethidine on paraffin sections. I wonder if the fluorescence is lost by fixation (12hrs in 4% PAF) and paraffin embedding plus deparaffination. All the papers I reviewed used hydroethidine on frozen section but our facility does not have vibratome or cryostat. Please help Please Help. Fabrice ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Merced M Leiker Research Technician III Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA lei...@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] bone marrow fixative
Acetic Zinc Formalin Disclaimer: The information in this message is confidential. If you are not the intended recipient, do not disclose, copy, or distribute this message, and please immediately contact the sender. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: B5 fixative
Amy, We use the B+ fixative available from BBC Biochemical ( http://www.bbcus.com/products.html?pc=19pid=57 ) My pathologists love it! Michelle Message: 3 Date: Tue, 02 Mar 2010 14:12:04 -0500 From: Amy Farnan farn...@nehealth.com Subject: [Histonet] bone marrow fixative To: histonet@lists.utsouthwestern.edu Message-ID: 4b8d1cb4.26ed.00d...@nehealth.com Content-Type: text/plain; charset=US-ASCII Good afternoon everyone, In past years our lab used B-5 fixative on bone marrow biopsies for better immunostaining. With the chemical hazards of B-5 we switched to AZF fixative and have been using this for the past few years. I am just curious what everyone else is using for fixative on their bone marrows and how is your immunoreactivity? Maybe a better question is what is the preferred fixative for bone marrows? Have a nice day, Amy Farnan Histology Supervisor Northeast Health *** _ Hotmail: Trusted email with Microsoft’s powerful SPAM protection. http://clk.atdmt.com/GBL/go/201469226/direct/01/___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] HE stainer/coverslippers
We are using the Leica Autostainer XL, bridge and coverslipper (CV5030). I think they're great! Michelle _ Hotmail: Trusted email with Microsoft’s powerful SPAM protection. http://clk.atdmt.com/GBL/go/201469226/direct/01/___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] IF staining on peritoneal macrophages
Rat anti-mouse F480 from serotec works hwell. It is crucial that you use a secondary against rat IgG that is highly cross adsorbed to many species, including mouse. I suggest whole IgG of donkey anti-rat IgG from Jackson Immunoresearch. Just make sure to pick the version adsorbed against mouse. I would give you the catalog number except I am not at my computer. Andrea T. Hooper ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Processing of fatty breast tissue
Following adequate fixation of predominantly fatty breast tissue, what factors could lead to significant shrinkage of adipose tissue around a fibrous lesion (by ~ 50-80%) ? _ IM on the go with Messenger on your phone http://go.microsoft.com/?linkid=9712960___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] IF staining on peritoneal macrophages
Rat anti-mouse F480 from serotec works hwell. It is crucial that you use a secondary against rat IgG that is highly cross adsorbed to many species, including mouse. I suggest whole IgG of donkey anti-rat IgG from Jackson Immunoresearch. Just make sure to pick the version adsorbed against mouse. I would give you the catalog number except I am not at my computer. Andrea T. Hooper ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Processing of fatty breast tissue
You have assumed that processing is correct, but shrinkage is always caused by too fast dehydration, therefore the processing is not adequate. René J. --- On Wed, 3/3/10, Dinesh Sariya sari...@hotmail.com wrote: From: Dinesh Sariya sari...@hotmail.com Subject: [Histonet] Processing of fatty breast tissue To: histonet@lists.utsouthwestern.edu Date: Wednesday, March 3, 2010, 10:41 AM Following adequate fixation of predominantly fatty breast tissue, what factors could lead to significant shrinkage of adipose tissue around a fibrous lesion (by ~ 50-80%) ? _ IM on the go with Messenger on your phone http://go.microsoft.com/?linkid=9712960___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Free radical detection and cell death IHC
Sorry to post this again but had problems posting messages lately. Here is my problem, We do not have cryostat in our facility and I will like to test free radical production in situ in rat brain tissue Some of the methods involves homogenate of whole part of brain and assay with component reacting with free radical that does not give you the geographical distribution of free radical. I'm therefore looking for a methods involving IHC or IF in paraffin section. I checked for hydroethidine but it seems like the fluorescence fades with paraffin processing. Has any one used hydroethidine with paraffin processing ? Does anyone know another method of free radical or oxidative stress detection on paraffin section ? We also wanted to asses cell death by some marker with the same tissue (paraffin processed) the caspase stain is faint and we will like to find a selective marker of cellular death or irreversible damage (apoptotic or non apoptotic) that could be used in IHC and IF. Has anyone ever used PI on rat tissue after paraffin processing ? Any other marker ? I heard about the hydroxyprobe but does it work on paraffin embedded tissue ? Thanks for your help guys Fabrice ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] HE stainer/coverslippers
I second the Prisma with attached coverslipper. We have been using it now for the past 2 months or so. It's really a non-hassle, state of the art piece of equipment. Paula -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Liz Chlipala Sent: Tuesday, March 02, 2010 12:50 PM To: histonet@lists.utsouthwestern.edu; VickiGauch Subject: RE: [Histonet] HE stainer/coverslippers We just purchased the sakura prisma and attached glass coverslipper. We just love them. We tried a lot of glass coverslippers and this by far is the best one that we have tried. We run both HE and specials on the prisma and have not had a problem with the glass coverslipper. We have been using them for about a little over 2 months now. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Tuesday, March 02, 2010 1:39 PM To: histonet@lists.utsouthwestern.edu; VickiGauch Subject: Re: [Histonet] HE stainer/coverslippers Sakura René J. --- On Tue, 3/2/10, Gauch, Vicki gau...@mail.amc.edu wrote: From: Gauch, Vicki gau...@mail.amc.edu Subject: [Histonet] HE stainer/coverslippers To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Date: Tuesday, March 2, 2010, 2:20 PM Hi, I was wondering what type of HE stainer/coverslipper units labs are using and the pros and cons of each. We found out we have funding to replace our old faithful GLX stainer(though it will break my heart to do so) and are looking for a stainer/ coverslipper that is one unit- one that we do not have to unload from the stainer to load onto the coverslipper. Any help would be greatly appreciated. Thanks so much, Vicki Gauch AMCH Albany,NY - CONFIDENTIALITY NOTICE: This email and any attachments may contain confidential information that is protected by law and is for the sole use of the individuals or entities to which it is addressed. If you are not the intended recipient, please notify the sender by replying to this email and destroying all copies of the communication and attachments. Further use, disclosure, copying, distribution of, or reliance upon the contents of this email and attachments is strictly prohibited. To contact Albany Medical Center, or for a copy of our privacy practices, please visit us on the Internet at www.amc.edu. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Ultram Fixative
Ultram Fixative is actually Ultrum Fixative and is available thru American Mastertech: http://www.americanmastertech.com/store/main.aspx?p=ItemDetailStylesitem=FXULTGAL Cindy Dubois Message: 20 Date: Tue, 2 Mar 2010 20:08:23 -0500 From: Robert Richmond rsrichm...@gmail.com Subject: [Histonet] Re: Ultram fixative To: histonet@lists.utsouthwestern.edu Message-ID: abea52a61003021708q3a5e96a5l7d23d18732547...@mail.gmail.com Content-Type: text/plain; charset=ISO-8859-1 Richard Cartun asks We have a clinic that is fixing tissue in Ultram. I have never heard of it. Can someone educate me about Ultram? I can't Google any evidence for an Ultram fixative. Ultram is a trade name for tramadol, an opioid analgesic. Either somebody is confused, or you've got somebody involved in dubious drug transactions. Can anybody in the clinic shed any light on the matter? Bob Richmond Samurai Pathologist Knoxville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] negative controls
we have a ventana benchmark and use rabbit and mouse negative controls, is there anyone out there that uses a universal control and is that ok with your cap inspection? thanks so much antia dudley providence hosp mobile alabama _ Hotmail: Powerful Free email with security by Microsoft. http://clk.atdmt.com/GBL/go/201469230/direct/01/___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] negative controls
Anita, If you're referring to the reagents you use, we use a Universal Negative Control Serum (Biocare) on our negative control slides. We have 3 Ventana instruments and have never been cited. Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of anita dudley Sent: Wednesday, March 03, 2010 11:44 AM To: histo...@pathology.swmed.edu Subject: [Histonet] negative controls we have a ventana benchmark and use rabbit and mouse negative controls, is there anyone out there that uses a universal control and is that ok with your cap inspection? thanks so much antia dudley providence hosp mobile alabama _ Hotmail: Powerful Free email with security by Microsoft. http://clk.atdmt.com/GBL/go/201469230/direct/01/ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] HE stainer/coverslippers
I am glad you decided on that one Paula. It is wonderful Phyllis Thaxton HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL From: Paula Lucas plu...@biopath.org To: Liz Chlipala l...@premierlab.com; histonet@lists.utsouthwestern.edu; VickiGauch gau...@mail.amc.edu Sent: Wed, March 3, 2010 10:50:06 AM Subject: RE: [Histonet] HE stainer/coverslippers I second the Prisma with attached coverslipper. We have been using it now for the past 2 months or so. It's really a non-hassle, state of the art piece of equipment. Paula -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Liz Chlipala Sent: Tuesday, March 02, 2010 12:50 PM To: histonet@lists.utsouthwestern.edu; VickiGauch Subject: RE: [Histonet] HE stainer/coverslippers We just purchased the sakura prisma and attached glass coverslipper. We just love them. We tried a lot of glass coverslippers and this by far is the best one that we have tried. We run both HE and specials on the prisma and have not had a problem with the glass coverslipper. We have been using them for about a little over 2 months now. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Tuesday, March 02, 2010 1:39 PM To: histonet@lists.utsouthwestern.edu; VickiGauch Subject: Re: [Histonet] HE stainer/coverslippers Sakura René J. --- On Tue, 3/2/10, Gauch, Vicki gau...@mail.amc.edu wrote: From: Gauch, Vicki gau...@mail.amc.edu Subject: [Histonet] HE stainer/coverslippers To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Date: Tuesday, March 2, 2010, 2:20 PM Hi, I was wondering what type of HE stainer/coverslipper units labs are using and the pros and cons of each. We found out we have funding to replace our old faithful GLX stainer(though it will break my heart to do so) and are looking for a stainer/ coverslipper that is one unit- one that we do not have to unload from the stainer to load onto the coverslipper. Any help would be greatly appreciated. Thanks so much, Vicki Gauch AMCH Albany,NY - CONFIDENTIALITY NOTICE: This email and any attachments may contain confidential information that is protected by law and is for the sole use of the individuals or entities to which it is addressed. If you are not the intended recipient, please notify the sender by replying to this email and destroying all copies of the communication and attachments. Further use, disclosure, copying, distribution of, or reliance upon the contents of this email and attachments is strictly prohibited. To contact Albany Medical Center, or for a copy of our privacy practices, please visit us on the Internet at www.amc.edu. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] AutoZyme
Hi, I am from Argentina and I have to perform an immunehistochemstry with an antigen recovery using AutoZyme from Biomedia Foster City, CA. I looked for in google and I found a lot papers citations but I could find the company, Biomedia, to ask for a quotation. Has anybody got a contact from this company? Or an alternative product? Thanks in advance. Lorena Lic. Lorena Bendetti Laboratori de Terapia Molecular y Celular Fundacion Instituto Leloir Buenos Aires Argentina ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Colorado Society of Histotechnology meeting - April 23rd 24th in Estes Park
Hi, The Colorado Society of Histotechnology (CSH) meeting will be held at the YMCA or Rockies in Estes Park, CO, April 23rd 24th, 2010. If you are interested in attending the meeting and have not registered, please visit our website at http://www.coloradohisto.org/2010/meeting.htm. Online registration is available and the society is now able to take credit card payment. Thank you and I look forward to seeing you at the meeting. Regards, John McGinley CSH Secretary - John N. McGinley Cancer Prevention Laboratory Colorado State University 1173 Campus Delivery Fort Collins, CO 80523-1173 Ph: (970) 491-3041 Fx: (970) 491-3542 john.mcgin...@colostate.edu www.cpl.colostate.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] IF staining on peritoneal macrophages
Hi Andrea, Jackson lists a couple of FITC-conjugated donkey anti-rat secondaries: 712-095-150http://www.jacksonimmuno.com/MERCHANT2/merchant.mv?Screen=PRODProduct_Code=712-095-150 - Whole Donkey Anti-Rat IgG (H+L). 712-096-150http://www.jacksonimmuno.com/MERCHANT2/merchant.mv?Screen=PRODProduct_Code=712-096-150 - F(ab')2 fragment Donkey Anti-Rat IgG (H+L). Do you recognize which is the one you have ben using. I have been using Serotec's STAR80F antibody. They suggest using 1:10 Normal Mouse Serum in PBS to make the necessary dilutions. Is this something you do with the Jackson antibodies as well? I've never heard of this technique before. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Ultram Fixative
According to the MSDS sheet at the web address provided by Cindy Dubois, this solution contains water, sodium acetate, zinc chloride, phenol and citric acid - concentrations not disclosed; pH not stated. Another MSDS for ultrum at http://siri.org/msds/f2/bzr/bzrjb.html lists the ingredients as 1,5-pentanedial (that's glutaraldehyde) 3%, zinc sulphate.7H2O 1%, carboxy hydroxide (=??!, but 0.04%) and buffers 2.0%. Solutions containing glutaraldehyde and phenol are used as disinfectants; all kinds of additives, not the ones listed for ultrum, are included to increase shelf life and reduce the corrosive effect on metals (see eg Schattner 1978 US Patent 410301). Phenol accelerates protein cross-linking by formaldehyde, and phenol-formaldehyde fixatives were sometimes used in the late 1980s to early 1990s (see Hopwood et al 1989 Histochem. J. 21:228-234). This mixture never became popular. A Scopus search shows only 6 articles citing the original publication. Glutaraldehyde fixation can be bad news for light microscopy because it leaves all parts of the tissue bristling with free aldehyde groups. These can bind some dyes, are Schiff-positive and can bind proteins such as antibodies. Glutaraldehyde also induces fluorescence (not, strictly speaking autofluorescence, but just as unwanted). There are various clever ways of overcoming these undesirable actions of glutaraldehyde (eg Kasten Lala 1975 Stain Technol. 50: 197-201; Tagliaferro et al 1997 J. Neurosci. Methods 77(2):191-197). There is no shortage of stable fixative mixtures with known composition and ingredients whose actions on tissues have been quite thoroughly studied, and which don't corrode metals. John Kiernan Anatomy, UWO London, Canada = = = - Original Message - From: Cindy DuBois integrated.hi...@gmail.com Date: Wednesday, March 3, 2010 12:17 Subject: [Histonet] Ultram Fixative To: histonet@lists.utsouthwestern.edu Ultram Fixative is actually Ultrum Fixative and is available thru American Mastertech: http://www.americanmastertech.com/store/main.aspx?p=ItemDetailStylesitem=FXULTGAL Cindy Dubois Message: 20 Date: Tue, 2 Mar 2010 20:08:23 -0500 From: Robert Richmond rsrichm...@gmail.com Subject: [Histonet] Re: Ultram fixative To: histonet@lists.utsouthwestern.edu Message-ID: abea52a61003021708q3a5e96a5l7d23d18732547...@mail.gmail.comContent-Type: text/plain; charset=ISO-8859-1 Richard Cartun asks We have a clinic that is fixing tissue in Ultram. I have never heard of it. Can someone educate me about Ultram? I can't Google any evidence for an Ultram fixative. Ultram is a trade name for tramadol, an opioid analgesic. Either somebody is confused, or you've got somebody involved in dubious drug transactions. Can anybody in the clinic shed any light on the matter? Bob Richmond Samurai Pathologist Knoxville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: paraffin processing times for brain
Hi all I was wondering if anyone can send me please the optimal paraffin processing times for brain autopsy tissue. Currently the blocks have cut and stained well but 4-5mm thick tissue has expanded (bulged out) following cutting as if they had absorbed water. Solutions been all changed. thanks Stephen |Stephen Kum Jew |Senior Technical Officer |Discipline of Pathology |School of Medical Sciences |Blackburn Building D06 |University of Sydney NSW 2006 |Australia |Ph: + 61 2 9036 9027 |Fax:+61 2 9351 3429 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: paraffin processing times for brain
we used to do one hour in each step formalin through to paraffin (2 steps of paraffin). But 4 to 5mm is a little on the thick side. We used to try for 3 mm. But didn't always get it that way. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Stephen Kum Jew [stev...@med.usyd.edu.au] Sent: Wednesday, March 03, 2010 3:42 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: paraffin processing times for brain Hi all I was wondering if anyone can send me please the optimal paraffin processing times for brain autopsy tissue. Currently the blocks have cut and stained well but 4-5mm thick tissue has expanded (bulged out) following cutting as if they had absorbed water. Solutions been all changed. thanks Stephen |Stephen Kum Jew |Senior Technical Officer |Discipline of Pathology |School of Medical Sciences |Blackburn Building D06 |University of Sydney NSW 2006 |Australia |Ph: + 61 2 9036 9027 |Fax:+61 2 9351 3429 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Meditech and Computer Requisitions vs Paper Req's
Our hospital uses Meditech and in the near future we are going to go to CPOE (computerized physician order entry). We currently are the only dept that uses paper requisitions for Surgical Specimens and Cytology. For those of you out there that has gone through this transition, can you share any issues you have had? Thank you. Lori A Disher Lead Histology Tech Fawcett Memorial Hospital 21298 Olean Blvd, Port Charlotte, FL 33952 phone 941-627-6128 fax 941-764-7071 lori.dis...@hcahealthcare.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Butyl alcohol
Has anyone tried using butyl alcohol as a dehydrating and clearing agent on there tissues? What are some pros and cons people have experienced with using Butyl alcohol? Todd Krueger HTL(ASCP)CM Boston Scientific 2 Scimed Place, P121 Osseo, MN 55311 Phone: 763-694-5709 Fax: 763-694-5505 e-mail: todd.krue...@bsci.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Ultrum II (Ultram) fixative
Richard Cartun asked about Ultram fixative, which Cindy Dubois informs us is actually Ultrum fixative. According to its MSDS (dated 2003), Ultrum II tissue fixative contains glutaraldehyde, phenol, zinc chloride, sodium acetate, citric acid, and water. It is offered by American Master*Tech Scientific, Inc. in Lodi CA. http://www.americanmastertech.com/histologysupply.htm They also supply genuine B5 fixative, Bouin's fixative, and Carnoy's fixative. Some patent applications from a few years back suggest that Ultrum II was used to fix prostate biopsy specimens. Obviously glutaraldehyde and phenol both have safety and environmental problems of their own. Whether they would practically and legally support IHC would be hard to predict. And in diagnosing prostate cancer, the use of nucleoli as a criterion of malignancy requires NBF - other fixatives are likely to result in demonstrating nucleoli in benign cells. Bob Richmond Samurai Pathologist Knoxville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] VIP basket for K1000
Hi Netters!! Does anyone have an extra basket that I can borrow or purchase for the K1000 VIP? Doctor wants to start processing but basket wasn't included in processor. It's the small one. Thanks!!! I am in AZ if anyone here has one, I can come pick up.. Thanks!! Jill Jill Cox HT (ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Alcohol Source?
I vote for the scotch! Claire depends. A fine Scotch whiskey may be aged in old oak casks, while a delicate Tequila may not be ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Re: bone marrow fixative
Ah, in a perfect world! (which would be down right boring, thanks) Claire Neutral buffered formalin requires time for fixation - clots need to be cut up as soon as possible after they're received, and biopsy specimens really ought to fix overnight before decalcification and processing. Communication with oncologists is essential (and rarely achievable). Bob Richmond Samurai Pathologist Knoxville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Butyl alcohol
Dear Todd Krueger, There are three isomeric butyl alcohols (primary, secondary and tertiary) with different physical properties. and different uses in histology for primary and tertiary. I have used tertiary butyl alcohol (= t-butanol = 2-methylpropan-2-ol) a few times for processing into paraffin. It mixes with with both water and wax. It boils at 83C so it's slightly less of a fire hazard than ethanol. It doesn't form explosive peroxides with long storage, which makes it safer than dioxane and tetrahydrofuran, two other universal solvents that have been used for combined dehydration and clearing. t-Butanol doesn't have an offensive odour, but it is solid below 25C, which is inconvenient, and it's quite a bit more expensive than more commonly used solvents such as ethanol, isopropanol and xylene. t-Butanol was introduced as a combined dehydration and clearing agent by Larbaud (1921) Compt. Rend. Acad. Sci. 172:1317-1319. It is used more in plant than in animal histology. Primary butyl alcohol (n-butanol) is liquid at ordinary temperatures. It is only partly miscible with water, but miscible with ethanol-water mixtures and with paraffin. It has been recommended for transitioning to wax in procedures claimed to reduce hardening of wood (Zirkle 1930, Science 71:103-104) and insect specimens (Stiles 1934, Stain Technol. 9:97-100). Freeze-substitution into n-butanol can be followed by paraffin embedding because this alcohol is a liquid from -90 to +117C. I haven't tried any of these methods. Another use of n-butanol is in dehydration of sections stained with dyes that are easily extracted by water or water-ethanol mixtures. I have lots of experience in this area. A major disadvantage of n-butanol, for any application, is its vapour. It doesn't smell nasty but it makes you cough. John Kiernan Anatomy, UWO London, Canada = = = - Original Message - From: Krueger, Todd todd.krue...@bsci.com Date: Wednesday, March 3, 2010 17:08 Subject: [Histonet] Butyl alcohol To: histonet@lists.utsouthwestern.edu Has anyone tried using butyl alcohol as a dehydrating and clearing agent on there tissues? What are some pros and cons people have experienced with using Butyl alcohol? Todd Krueger HTL(ASCP)CM Boston Scientific 2 Scimed Place, P121 Osseo, MN 55311 Phone: 763-694-5709 Fax: 763-694-5505 e-mail: todd.krue...@bsci.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
