Re: [Histonet] Used knife sharpeners
Caroline, You can buy a new or refurbished HI-76 knife sharpener thru Hacker Instruments and it will come with a warranty. If you find one on Ebay, you can get the replacement honing and stropping wheels from Hacker. ( I know, it's quite the name for a company that sells knife sharpeners). The HI-76 comes with an instructional video. It's the one thing that is usually missing when you acquire used equipment. The guy you need to talk to at Hacker is Jim Mullen 800-442-2537. Good luck with your search. Dorothy Dorothy Traczyk MTA Histology LLC PO Box 602 Point Pleasant, NJ 08742 T: 732-899-2912 F: 732-899-5469 www.mtahistology.com In a message dated 3/28/2010 1:24:21 P.M. Eastern Daylight Time, cb...@wfubmc.edu writes: Hey Guys, What¹s the most convenient and economical way to sharpen a knife for an AO860? I know there are commercial services you can send the knife to. I¹ve also seen used knife sharpeners on ebay. Since my AO microtome is so nice, I¹m tempted to get one of the AO knife sharpeners (like the 935). Any recommendations on what to buy or look for in a used knife sharpener? Any recommendations for a book or website on basic knife sharpening techniques? Thanks, Caroline Bass ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] mouse perfusion rate
Hi Joe, Thanks for that notice about flow rates. But I think for the mouse you meant 1-3mls/min (not per 10min?)... Regards, Merced --On Saturday, March 27, 2010 5:03 PM -0700 Joseph Saby saby_josep...@yahoo.com wrote: All- From previous work with rat perfusions, the flow rate was about 10 ml/minute. If I had to guess, the equivalent flow rate for a mouse would be closer to 1-3 mls/10 minutes. If you go 10 ml/minute, you will definitely cause blowout artefacts. Joe Saby, BA HT __ From: Merced M Leiker lei...@buffalo.edu To: charles.scou...@leica-microsystems.com; mak...@ufl.edu; histonet@lists.utsouthwestern.edu Sent: Fri, March 19, 2010 9:21:38 AM Subject: RE: [Histonet] mouse perfusion rate The vasculature will leak too much and the mouse will get bloated - you'll see it first in either the intestines blowing up like a balloon or fluid coming out of the nose. Just not the same as the heart pumping when the mouse is alive with intact physiology and normal functioning. Don't know exactly why, but that's what happens when you go too fast. Perhaps the vasculature has lost its control to compensate for the pressure? I'm not a physiologist so I'm not sure why...maybe someone on the Histonet can answer that? Regards, Merced --On Thursday, March 18, 2010 5:49 PM -0500 charles.scou...@leica-microsystems.com wrote: Why not? What happens? One would think the mammalian cardiovascular system could withstand physiological pressures and flow rates, at least for one lifetime? Cordially, Charles W. Scouten, Ph.D Product Manager, MNL Biosystems Division Leica Biosystems Richmond, Inc. 5205 Route 12 P.O. Box 528 Richmond, IL 60071 United States of America Telephone 630 964 0501 facsimile +1 630 964 0576 www.MyNeuroLab.com www.leica-microsystems.com IMPORTANT - This email and any attachments may be confidential. Any retransmissions, dissemination or other use of these materials by persons or entities other than the intended recipient is prohibited. If received in error, please contact us and delete all copies. Before opening or using attachments, check them for viruses and defects. Our liability is limited to resupplying any affected attachments. [Any representations or opinions expressed in this email are those of the individual sender]. From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Merced M Leiker lei...@buffalo.edu Sent: Thursday, March 18, 2010 12:38 PM To: MKing mak...@ufl.edu; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] mouse perfusion rate That may be mouse cardiac output, but I can assure you, from experience, you do not want to perfuse at 17ml/min. Regards, Merced --On Thursday, March 18, 2010 1:32 PM -0400 MKing mak...@ufl.edu wrote: Li, Mouse cardiac output seems to be about 17 ml/min (e.g. www.transonic.com/mice1.shtml), you probably want to try for that to keep pressures close to physiological. A syringe pump is pretty inexpensive and probably all you need. Mike - Original Message - From: Li Zhang dancingw...@yahoo.com Date: Wednesday, March 17, 2010 14:59 Subject: [Histonet] question about mouse perfusion To: histonet@lists.utsouthwestern.edu My question is: can anyone give me a rough idea of how fast I should inject ( like ml/min). I think I've tried like 30 ml in 3 min, and I suspect that it's too fast because I do observe tissue swelling sometimes. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Merced M Leiker Research Technician III Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA lei...@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email __ Merced M Leiker Research Technician III Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA lei...@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Merced M Leiker
[Histonet] Removing tissues from OCT for molecular analysis
Hello, Can someone suggest a means of removing tissues from OCT for subsequent molecular analysis? I did not find anything in the archives describing this exactly. Thanks, Katie Katie Crosby Immunohistochemistry Cell Signaling Technology 3 Trask Lane Danvers, MA 01923 Phone: 978-867-2352 Fax: 978-867-2400 WEB Site: www.cellsignal.com Toll Free: 1-877-616-CELL (877-616-2355) 1-877-678-TECH (877-678-8324) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE:cover slips
I have tried several brands all have the same problem some worse than others. But even worse than the coverslips are the slides. I cut a lot of frozen sections on charged slides for fluorescent labeling the slides are dirty and the dirt gets trapped under the tissue as well as around it. The dirt is auto fluorescent. Sometimes it is hard to tell label from auto fluorescence. I have tried slides from multiple vendors and all seem to have the same problem. Does anyone have a good vendor for clean charged slides. Message: 15 Date: Wed, 24 Mar 2010 16:24:18 -0400 From: Helen Fedor hfe...@jhmi.edu Subject: [Histonet] Cover glass To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Message-ID: 3201cf51728f6048a24fa3afffeef1d316bdea3...@jhemtexvs3.win.ad.jhu.edu Content-Type: text/plain; charset=us-ascii Hello, Our lab does IHC staining in house and have had zero problems with our Fisher brand cover slips until recently. We've tried both Fisher and Corning cover slips and what appears to be dust or imperfections in the glass are found on both brands. This is creating false positives for us, because when the positive staining is low it is difficult to impossible to tell the imperfections from the staining itself. Any advice will be welcome. -- Helen *** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RELIA Special Job Alert for Managers and Supervisors 3-29-10
Hi Histonetters!, I have several exciting opportunities for experienced Managers, and Supervisors in hospital and private lab environments in several locations nationwide. These are some of the premier employers in the United States. The positions are of course full time and permanent. My clients offer excellent compensation, benefits and relocation assistance. Here are my leadership positions: Histology Manager - Long Island, NY NYS lic NOT req Lab Manager - Uniondale, NY Lab Manager - Tempe, AZ Lab Manager - Richmond,VA Histology Manager - Uniondale, NY Histology Manager - Tempe, AZ Histology Manager - Richmond,VA Histology Supervisor - Las Vegas, NV Histology Manager - Spokane, WA If you would like more information or know of someone else who might be interested, please contact me at rel...@earthlink.net or 866-607-3542. I am available to discuss the opportunity at your convenience including after hours. Thanks-Pam There are a lot of recruiters out there right now trying to work with histology professionals and I appreciate your support and respect your needs. Remember I offer over 25 years of experience as a recruiter and for over 6 years I have dedicated my practice solely to placing histology professionals like you. Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: rel...@earthlink.net mailto:rel...@earthlink.net http://home.earthlink.net/~relia1 www.myspace.com/pamatrelia http://www.myspace.com/pamatrelia ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Removing tissues from OCT for molecular analysis
What you should do ideally is sample you tissue for molecular studie first, then carry out frozen sections Malika ... Smile it confuses people ... On 29 Mar 2010, at 15:31, Katie Crosby kcro...@cellsignal.com wrote: I would like to remove tissues from blocks of OCT and perform molecule studies. I need to separate tissues from OCT. Thanks, Katie On Mar 29, 2010, at 10:21 AM, Malika Benatti wrote: Do you mean removing OCT from tissue ? ... Smile it confuses people ... On 29 Mar 2010, at 13:55, Katie Crosby kcro...@cellsignal.com wrote: Hello, Can someone suggest a means of removing tissues from OCT for subsequent molecular analysis? I did not find anything in the archives describing this exactly. Thanks, Katie Katie Crosby Immunohistochemistry Cell Signaling Technology 3 Trask Lane Danvers, MA 01923 Phone: 978-867-2352 Fax: 978-867-2400 WEB Site: www.cellsignal.com Toll Free: 1-877-616-CELL (877-616-2355) 1-877-678-TECH (877-678-8324) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Katie Crosby Immunohistochemistry Cell Signaling Technology 3 Trask Lane Danvers, MA 01923 Phone: 978-867-2352 Fax: 978-867-2400 WEB Site: www.cellsignal.com Toll Free: 1-877-616-CELL (877-616-2355) 1-877-678-TECH (877-678-8324) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] mouse perfusion rate
I have perfused mice and rats at 300 mm Hg, about double physiological level, don't know what that made the flow rate. All mammals have the same blood pressure (within tolerances), so it is easier to select a suitable pressure to use than a flow rate, which varies dramatically. I look at brain, never pay any attention to the gut. Clear fluid comes out the nose, that is a good sign. There are pressure release valves across the cribiform plate to release CSF if there is too much. I am flooding the system, fluid coming out the nose means the extracellular fluid and CSF is being replaced as well as vascular blood. Good. The tissue is quality is excellent, free of red blood cells, can be unshrunk depending on the tonicity (should be sub isotonic) of the fixative fluid. Have looked at Nissl and EM material, no evidence of damage to the tissue. If gut is extended, might have something to do with the large intestines job of removing fluid from feces, and flooding the system swells the tissue. But does it matter? Do you use that tissue? What is the tissue quality if you use it after physiological pressure perfusion. Cordially, Charles W. Scouten, Ph.D Product Manager, MNL Biosystems Division Leica Biosystems Richmond, Inc. 5205 Route 12 P.O. Box 528 Richmond, IL 60071 United States of America Telephone 630 964 0501 facsimile +1 630 964 0576 www.MyNeuroLab.com http://www.myneurolab.com/ www.leica-microsystems.com http://www.leica-microsystems.com/ IMPORTANT - This email and any attachments may be confidential. Any retransmissions, dissemination or other use of these materials by persons or entities other than the intended recipient is prohibited. If received in error, please contact us and delete all copies. Before opening or using attachments, check them for viruses and defects. Our liability is limited to resupplying any affected attachments. [Any representations or opinions expressed in this email are those of the individual sender]. From: Merced M Leiker lei...@buffalo.edu [mailto:Merced M Leiker lei...@buffalo.edu] Sent: Monday, March 29, 2010 9:05 AM To: Joseph Saby saby_josep...@yahoo.com; charles.scou...@leica-microsystems.com; mak...@ufl.edu; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] mouse perfusion rate Hi Joe, Thanks for that notice about flow rates. But I think for the mouse you meant 1-3mls/min (not per 10min?)... Regards, Merced --On Saturday, March 27, 2010 5:03 PM -0700 Joseph Saby saby_josep...@yahoo.com wrote: All- From previous work with rat perfusions, the flow rate was about 10 ml/minute. If I had to guess, the equivalent flow rate for a mouse would be closer to 1-3 mls/10 minutes. If you go 10 ml/minute, you will definitely cause blowout artefacts. Joe Saby, BA HT __ From: Merced M Leiker lei...@buffalo.edu To: charles.scou...@leica-microsystems.com; mak...@ufl.edu; histonet@lists.utsouthwestern.edu Sent: Fri, March 19, 2010 9:21:38 AM Subject: RE: [Histonet] mouse perfusion rate The vasculature will leak too much and the mouse will get bloated - you'll see it first in either the intestines blowing up like a balloon or fluid coming out of the nose. Just not the same as the heart pumping when the mouse is alive with intact physiology and normal functioning. Don't know exactly why, but that's what happens when you go too fast. Perhaps the vasculature has lost its control to compensate for the pressure? I'm not a physiologist so I'm not sure why...maybe someone on the Histonet can answer that? Regards, Merced --On Thursday, March 18, 2010 5:49 PM -0500 charles.scou...@leica-microsystems.com wrote: Why not? What happens? One would think the mammalian cardiovascular system could withstand physiological pressures and flow rates, at least for one lifetime? Cordially, Charles W. Scouten, Ph.D Product Manager, MNL Biosystems Division Leica Biosystems Richmond, Inc. 5205 Route 12 P.O. Box 528 Richmond, IL 60071 United States of America Telephone 630 964 0501 facsimile +1 630 964 0576 www.MyNeuroLab.com www.leica-microsystems.com IMPORTANT - This email and any attachments may be confidential. Any retransmissions, dissemination or other use of these materials by persons or entities other than the intended recipient is prohibited. If received in error, please contact us and delete all copies. Before opening or using attachments, check them for viruses and defects. Our liability is limited to resupplying any affected attachments. [Any representations or opinions expressed in this email are those of the individual sender]. From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Merced M Leiker lei...@buffalo.edu Sent: Thursday,
[Histonet] Coverglass
We are currently trying to find a manufacturer that may sell cover glass slips measuring 45 x 60. We found a box by Clay Adams, but can not find that size anymore in their catalog. Debra Ann Ortiz Chief Medical Technologist The University of Chicago Medical Center Room E-602-A 5841 S. Maryland Avenue Chicago, Il 60637 phone: 773.702.5237 This e-mail is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged and confidential. If the reader of this e-mail message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is prohibited. If you have received this e-mail in error, please notify the sender and destroy all copies of the transmittal. Thank you University of Chicago Medical Center ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Is my Leica CM 1850 cryostat haunted?
We have a Leica CM 1850 cryostat that about once every three weeks to a month, turnes itself off! Has anyone else experienced this? How do I fix it? Initially, we thought someone after hours was turning it off, maybe someone in housekeeping or a passing med tech. That was not the case. Later our Biomedical people replaced a capacitor or something in the bowels of the cryostat thinking that may be faulty and contribute to the problem. That did not help either. Still later, the cryostat was put on its own circuit (No help), and then after that, we tried an uninterruptible power source thinking minor power fluctuations were the cause (no help either). Anyone know how to fix this problem?. It is disconcerting to walk into the lab in the morning and the cryostat is at room temperature and the OR is going to have a busy day. Michael Titford Pathology USA Mobile AL ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Acetone Fixation
Atoska Gentry asked: hello, if any of you have acetone fixation incorporated into you frozen section H E staining protocol will you please advise me on adjustments necessary for routine staining protocol. Also, out of curiosity please enlighten me on the purpose for post sectioning acetone fixation on tissue samples initially fixed in 95% ETOH/ Acetic Acid? Your prompt replies will be greatly appreciated. ~ Atoska Several thoughts come to mind. Acetone fixation is usually used only for preserving antigenicity on fresh frozen tissue samples. For slides needing HE I would use a formalin fixative prior to the HE procedure for fresh frozen tissues. Acetone fixed HE stained cryosections are uuugly. You should not be doing frozen sections on tissues initially fixed in alcohol. Alcohol is an anti-freeze and you will not get good freezing/sectioning of the samples. You can section unfixed samples and then fix after mounting on glass slides in the Alcohol/acetic acid fix. Teri Johnson, HT(ASCP)QIHC Managing Director, Histology Facility Stowers Institute for Medical Research Kansas City, MO ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] osteopontin
Can anyone recommend an osteopontin antibody for inflammation that works in FFPE mouse tissue, in particular mouse paws? This communication is intended solely for the use of the addressee and may contain information that is legally privileged, confidential or exempt from disclosure. If you are not the intended recipient, please note that any dissemination, distribution, or copying of this communication is strictly prohibited. Anyone who receives this message in error should notify the sender immediately and delete it from his or her computer ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Learn From the Best, Head West!
The Arizona Society for Histotechnology (ASH) is hosting the Region VII Summer Symposium June 4 - June 6 2010 at the Embassy Suites Phoenix North, 2577 W. Greenway Road, Phoenix, AZ 85023. Attendees with paid registration by May 15, 2010 will get a chance to win $100.00 cash. Guest speakers: Friday Jun 4 8 am - 11:30 am - Basic Chemistry and Lab Math for the HT, presented by Ada Feldman - LEAN Principles in Histology, presented by William DeSalvo - Immuno-staining of Cytological Materials: Application and Pitfalls, presented by Joseph Myers Friday Jun 4 1:00 pm - 4:30 pm - Rapid Processing: Microwave Style, presented by Donna Willis - It's your Health; It's your Safety, Julia Rosen and Dan Williams - This Doesn't Happen When I Stain Manually!, Peggy McArthur Saturday Jun 5 8 am - 11:30 am - Benefits of Multi-antigen Immuno-Staining, presented by Joseph Myers - HT/HTL Exam PART I, presented by Robert Lott - Knowing You, Knowing Me, presented by Beth Roche and Roger Strickland Saturday Jun 5 1:30 pm - 5:00 pm - Quality Control and Standardization of IHC, Kelsey Jones - HT/HTL Exam PART II, Robert Lott - Novel Detection Chemistry, Traci DeGeer Sunday Jun 6 8:00 am - 11:30 am - Tissue identification Made Easy, presented by Ada Feldman - Antibody Challenge 2010, Kelsey Jones and Ourhay Shamoon - Water Quality and Standards for the Histology Laboratory, Ethel Macrea Special events planned for Friday and Saturday evenings. For detailed brochure e-mail mshaef...@cox.net See you there, Marc Shaeffer ASH Secretary ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] MO Society for Histotechnology 2010 Spring Symposium
Hi all! The Missouri Society for Histotechnology cordially invites you to our 2010 Spring Symposium for continuing education and histopathology technique and earn up to 12 CEUs. This year's conference will be held in: Saint Louis, Missouri Thursday, May 20th to Saturday, May 22nd We will stay at the Sheraton Westport Lakeside Chalet which is located on Westport Plaza, a centrally located 42 acres development offering an unparalleled combination of amenities and services, where you may enjoy over 20 restaurant and entertainment venues. It is nestled in the heart of St Louis and surrounded by everything exciting that the city has to offer. Room reservations can be made by calling 1-800-822-3535. Hotel reservation deadline for symposium rate is April 29, 2010. Reservations received after this date will be subject to room rate availability. To secure your symposium rate please make your reservations early and indicate that you are attending the MSH symposium. Online room registration and meeting information is available at www.starwoodmeeting.com/Book/mohistotechnology. Visit our webpage for more information and links to the Symposium at www.missouri-histo.org/index.cfm For registration and more information about the program and work shop descritions, download the Brochure 2010 and Workshop Descriptions MHT 2010 in our downloads page. Obtain a discounted price on the symposium registration fee by becoming an active member of the society. Download a Membership/Renewal Application, fill it up and send it with your dues and your registration for the symposium. For more information, contact Amanda Kelley, MSH President, at amanda.kel...@stlukes-stl.com or Sharon Walsh at userwa...@sbcglobal.net Hope to see you there! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Removing tissues from OCT for molecular analysis
Katie, Rinse the frozen OCT surrounded tissue in Hanks or similar cell culture fluid. Two to three times should be enough to remove the OCT Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Katie Crosby Sent: Monday, 29 March 2010 11:56 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Removing tissues from OCT for molecular analysis Hello, Can someone suggest a means of removing tissues from OCT for subsequent molecular analysis? I did not find anything in the archives describing this exactly. Thanks, Katie Katie Crosby Immunohistochemistry Cell Signaling Technology 3 Trask Lane Danvers, MA 01923 Phone: 978-867-2352 Fax: 978-867-2400 WEB Site: www.cellsignal.com Toll Free: 1-877-616-CELL (877-616-2355) 1-877-678-TECH (877-678-8324) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet * This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. * ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
