RE: [Histonet] biohazard bags
I agree. We never re-use them at my facility. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu]on Behalf Of Stacy McLaughlin Sent: Thursday, July 22, 2010 3:38 PM To: Jeff Birkner; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] biohazard bags Re-use of specimen transport bio-bags is a big NO-NO in my laboratory. You run the risk of accidentally using one that is contaminated. (This actually happened, and it was not a pretty sight.) You can't guarantee that the outside of that specimen container in the bag is not contaminated, even though it looks clean. You don't know the practices of the person that collected it. This could be a big liability and the possible consequences just aren't worth it. Just my 2 cents. Stacy -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu]on Behalf Of Jeff Birkner Sent: Thursday, July 22, 2010 3:11 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] biohazard bags We are looking into our current use of biohazard bags. How many of you are currently re-using any bags for specimen transport? These would only be bags were the actual specimen was inside a secondary container and thus never directly came into contact with the bags. Thanks! Jeffrey C. Birkner, CT(ASCP) Manager, Pathology Laboratory Section Collaborative Laboratory Services, L.L.C. 1005 Pennsylvania Ave, Suite 102 Ottumwa, IA 52501 641-455-5414 ORHC Extension #3538 jbirk...@colabserv.com The information contained in this communication may be confidential, is intended only for the use of the recipient(s) named above, and may be legally privileged. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication, or any of its contents, is strictly prohibited. If you have received this communication in error, please return it to the sender immediately and delete the original message and any copy of it from your computer system. If you have any questions concerning this message, please contact the sender. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] p-TBK-1 Ab
Good morning everyone, I work in a research lab and am trying to optimize a p-TBK-1 antibody; I am running this work-up on a BondIII and a BondMax with a Dako Envision Rabbit kit for detection and secondary. I have only seen very faint staining and am wondering if anyone out there has worked with this and, if so, what type of control tissue would show the best signal. Thanks for any help, Michelle Mathews, HT(ASCP) TPL, UNC-CH ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Artifacts in histology section
Dear Friends, I am an Histology Technologist. I am having a problem here,while sectioning am not seeing and scoring artifacts on the section but in the microscope am seeing a tearing artifacts particularly in endoscopy biopsies. am not able to locate where is the problem,is that because of blades or due to micro-crystallization of wax or due to any processing problem. Its not consistently in all but i get it on some blocks every day. Can any one help me in sorting it out. If anybody is interested in will send the picture of those section. with regards, Aazathraj.P Technical Officer, Apollo Hospitals-chennai India. aaz...@hotmail.com _ The latest in fashion and style in MSN Lifestyle http://lifestyle.in.msn.com/___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Microwave Decalcification
Hello all, We inherited a Pelco Biowave 34700 (from Ted Pella) and would like to optimize decalcification of rats knee joints, dogs rib using the Biowave. I was wondering if anyone have a protocol for decalcification using nitric acid or formic acid or EDTA using microwave or if you know someone that uses microwave technology for decalcification that I can ask. Your help is appreciated. Leticia ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Artifacts in histology section
Of the 3 probable causes, the most likely to be is the blade edge not uniformly sharp René J. --- On Fri, 7/23/10, Aazath Raj aaz...@hotmail.com wrote: From: Aazath Raj aaz...@hotmail.com Subject: [Histonet] Artifacts in histology section To: histonet@lists.utsouthwestern.edu Date: Friday, July 23, 2010, 11:26 AM Dear Friends, I am an Histology Technologist. I am having a problem here,while sectioning am not seeing and scoring artifacts on the section but in the microscope am seeing a tearing artifacts particularly in endoscopy biopsies. am not able to locate where is the problem,is that because of blades or due to micro-crystallization of wax or due to any processing problem. Its not consistently in all but i get it on some blocks every day. Can any one help me in sorting it out. If anybody is interested in will send the picture of those section. with regards, Aazathraj.P Technical Officer, Apollo Hospitals-chennai India. aaz...@hotmail.com _ The latest in fashion and style in MSN Lifestyle http://lifestyle.in.msn.com/___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Formalin substitutes
Hi everyone - does anyone know how effective these formalin substitutes are at killing microorgansms9esp HG3) in the tissue fixed? I know that Finefix states that because of the alcohol content being over 70% that it is effective against many micro-organisms but info seems scarce on the others Thanks and have a nice weekend Colin Weaver Histology lab manager VLA Thirsk North Yorkshire UK /prebrVeterinary Laboratories Agency (VLA)brbrThis email and any attachments is intended for the namedbrrecipient only.brIf you have received it in error you have no authority to use,brdisclose, store or copy any of its contents and you should destroybrit and inform the sender. Whilst this email and associated attachments will have beenbrchecked for known viruses whilst within VLA systems we canbraccept no responsibility once it has left our systems.brCommunications on VLA's computer systems may be monitoredbrand/or recorded to secure the effective operation of the systembrand for other lawful purposes.brpre ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] artifacts of sectioning
What I have used in the past is a short warm water soak, 10-15 seconds, in the water bath, after facing in and between sections, then placing the block back on ice. I would use a small 200 mL beaker filled with DI water sitting inside the water, it helps reduce junk floating the bath. Be careful to avoid over soaking. Matt Brooks, BS, HT (ASCP) Histology Supervisor InCyte Pathology mbro...@incytepathology.com 509-892-2744 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tench, Bill Sent: Friday, July 23, 2010 9:10 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] artifacts of sectioning It sounds like what you are describing is what we call chatter. it seems to occur most frequently with GI biopsies, esp colon polyps and usually in the abnormal epithelium. My techs tell me that cooling the block better on the ice block makes it go away. i have no personal experience, other than to say that when it occurs, it makes the slide of very poor quality. Bill Tench Associate Dir. Laboratory Services Chief, Cytology Services Palomar Medical Center 555 E. Valley Parkway Escondido, California 92025 bill.te...@pph.org Voice: 760- 739-3037 Fax: 760-739-2604 [None] made the following annotations - NOTICE: This email message is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. This message has been content scanned by the Axway MailGate. MailGate uses policy enforcement to scan for known viruses, spam, undesirable content and malicious code. For more information on Axway products please visit www.axway.com. - ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] HPV
We are doing the P16 antibody from MTM Labs. Rick Garnhart HT(ASCP) Memorial Health System Histology Supervisor 1400 E. Boulder St. Colorado Springs, CO 80909 Cell: 719-365-8357 Ph: 719-365-6926 Fax: 719-365-6373 rick.garnh...@memorialhealthsystem.com Mission: To provide the highest quality health care Vision: To create an outstanding health system where patients heal and people thrive Values: Compassion - Integrity - Quality - Respect - Teamwork www.memorialhealthsystem.com The information contained in or attached to this electronic message is privileged and confidential, intended only for the use of the individual(s) named above. If the reader of this message is not the intended recipient, or the employee or agent responsible to deliver it to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please inform the sender immediately and remove any record of this message.___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Water collecting at bottom of sections
Hello all, From time to time and depending on what brand of adhesive (or charged) slides I am using, I seem to get a bag of water that drains to the bottom of my sections but doesn't drain out. I have been working in microtomy a long time and have had to deal with this contingency time and time again, but never really have gotten to the bottom of the problem. I spoke with a premium manufacturer of such slides and they seemed to indicate that it is a problem with the coating, but couldn't tell me for sure. All I know is that certain brands do this more than others. If you know what I mean, you know it is a problem. My bath is pure distilled H2O with no gelatin or Sta-on added. It is if the adhesive properties are SO good that they will not release the water when vertically drained and have to be shaken off or cut with a razor blade at bottom to release the water. Anyway, if anyone has an insight or two on this, I would be interested. It seem sthe most challenging issues are ones that seem related to some of the most simple tasks that one has performed for many years!! Manufacturers understand what I mean, but cannot pinpoint the problem for me via phone or e-mail. Anyone see this and have a chemical/mechanical solution they have developed over the years? Thanks! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] thick slice IHC help!!
Hi all, I'm a summer student and the project I was given was to optimize the immunohistochemistry in my lab. BUT I'M NOT GETTING ANYTHING TO WORK!! I've been told it always worked beforeSo I was wondering if anyone has any ideas or protocols that work? I'd really appreciate any help I can get! Here's the protocol I'm using: our slices are 250-300 um thick. 1) I leave them in formalin for 2 days for the fixative step. 2) Wash slices 3X (15 min each) in PBS-T (PBS with 0.3% tritonX100 to help permeabilize my slices since they're thick) 3) Blocking step for 1/2 hr with 2%normal horse serum in PBS-T 4) Incubate in 1o antibody at 1:1000 in PBS-T + 2%normal horse serum for 72 hrs at 4C with shaking 5) Wash slices 5X in PBS-T 6) Blocking step for 1/2 hr with 2%normal horse serum in PBS-T 7) Incubate at room temp with 2o antibody at 1:500 +2%normal horse serum in PBS-T for 2hrs with shaking and kept in the dark 8 ) Wash 5X (1/2 hr each) in PBS before mounting Terri ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Water collecting at bottom of sections
I routinely flick my wrist holding the slide with the section I've just picked up. Usually, this is enough force to release any water at the bottom of the section. If that doesn't work I melt a tiny hole at the bottom of the section with a heated probe and flick again. I'm sure the manufacturer is right in saying that its the coating; the paraffin adheres too well and too quickly to the slide trapping water underneath. It is also very important to hold your slides as vertically as possible when bringing them under your section and raising the slide out of the water bath. That way you will trap as little water as possible underneath. Hope this helps. From one long-time-microtomist to another, Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of nap...@mail.siscom.net Sent: Friday, July 23, 2010 1:04 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Water collecting at bottom of sections Hello all, From time to time and depending on what brand of adhesive (or charged) slides I am using, I seem to get a bag of water that drains to the bottom of my sections but doesn't drain out. I have been working in microtomy a long time and have had to deal with this contingency time and time again, but never really have gotten to the bottom of the problem. I spoke with a premium manufacturer of such slides and they seemed to indicate that it is a problem with the coating, but couldn't tell me for sure. All I know is that certain brands do this more than others. If you know what I mean, you know it is a problem. My bath is pure distilled H2O with no gelatin or Sta-on added. It is if the adhesive properties are SO good that they will not release the water when vertically drained and have to be shaken off or cut with a razor blade at bottom to release the water. Anyway, if anyone has an insight or two on this, I would be interested. It seem sthe most challenging issues are ones that seem related to some of the most simple tasks that one has performed for many years!! Manufacturers understand what I mean, but cannot pinpoint the problem for me via phone or e-mail. Anyone see this and have a chemical/mechanical solution they have developed over the years? Thanks! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Water collecting at bottom of sections
I experienced it almost daily and thought this was normal!? I just dipped the slides gently on a paper towel right after getting them on the slide and shaking, for the case I could not just shake it off. Indeed, as I remember, the Super Frost Plus slides had to be dipped a bit harder to let the water rinse off. Hello all, From time to time and depending on what brand of adhesive (or charged) slides I am using, I seem to get a bag of water that drains to the bottom of my sections but doesn't drain out. I have been working in microtomy a long time and have had to deal with this contingency time and time again, but never really have gotten to the bottom of the problem. I spoke with a premium manufacturer of such slides and they seemed to indicate that it is a problem with the coating, but couldn't tell me for sure. All I know is that certain brands do this more than others. If you know what I mean, you know it is a problem. My bath is pure distilled H2O with no gelatin or Sta-on added. It is if the adhesive properties are SO good that they will not release the water when vertically drained and have to be shaken off or cut with a razor blade at bottom to release the water. Anyway, if anyone has an insight or two on this, I would be interested. It seem sthe most challenging issues are ones that seem related to some of the most simple tasks that one has performed for many years!! Manufacturers understand what I mean, but cannot pinpoint the problem for me via phone or e-mail. Anyone see this and have a chemical/mechanical solution they have developed over the years? Thanks! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Water collecting at bottom of sections
I second Linda's technique, the only other option I use is to bring the slide up at a negative angle so the slide comes out of the water bath first then the section. I find this works well for us. Cindy Pyse, CLT, HT (ASCP) Histology Supervisor X-Cell Laboratories e-mail cp...@x-celllab.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sebree Linda A Sent: Friday, July 23, 2010 2:42 PM To: nap...@mail.siscom.net; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Water collecting at bottom of sections I routinely flick my wrist holding the slide with the section I've just picked up. Usually, this is enough force to release any water at the bottom of the section. If that doesn't work I melt a tiny hole at the bottom of the section with a heated probe and flick again. I'm sure the manufacturer is right in saying that its the coating; the paraffin adheres too well and too quickly to the slide trapping water underneath. It is also very important to hold your slides as vertically as possible when bringing them under your section and raising the slide out of the water bath. That way you will trap as little water as possible underneath. Hope this helps. From one long-time-microtomist to another, Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of nap...@mail.siscom.net Sent: Friday, July 23, 2010 1:04 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Water collecting at bottom of sections Hello all, From time to time and depending on what brand of adhesive (or charged) slides I am using, I seem to get a bag of water that drains to the bottom of my sections but doesn't drain out. I have been working in microtomy a long time and have had to deal with this contingency time and time again, but never really have gotten to the bottom of the problem. I spoke with a premium manufacturer of such slides and they seemed to indicate that it is a problem with the coating, but couldn't tell me for sure. All I know is that certain brands do this more than others. If you know what I mean, you know it is a problem. My bath is pure distilled H2O with no gelatin or Sta-on added. It is if the adhesive properties are SO good that they will not release the water when vertically drained and have to be shaken off or cut with a razor blade at bottom to release the water. Anyway, if anyone has an insight or two on this, I would be interested. It seem sthe most challenging issues are ones that seem related to some of the most simple tasks that one has performed for many years!! Manufacturers understand what I mean, but cannot pinpoint the problem for me via phone or e-mail. Anyone see this and have a chemical/mechanical solution they have developed over the years? Thanks! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Water collecting at bottom of sections
I use FisherBrand SuperFrost Plus charged slides, the only time I have this problem is when my waterbath is too hot, or if for some reason I use warmed slides to retrieve my sections from the bath (like if you lay the unused slides on the edge of the waterbath, or if you use the warm droplet method to spread your sections)? I haven't had much experience with a lot of different slide types/brands though, but it might be an easy fix to the problem you hadn't thought about, as it is more or less unrelated to the brand of slide, although some brands may be more sensitive to this issue... Sincerely, Nicole Collette Lawrence Livermore National Lab/ UC Berkeley -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of nap...@mail.siscom.net Sent: Friday, July 23, 2010 11:04 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Water collecting at bottom of sections Hello all, From time to time and depending on what brand of adhesive (or charged) slides I am using, I seem to get a bag of water that drains to the bottom of my sections but doesn't drain out. I have been working in microtomy a long time and have had to deal with this contingency time and time again, but never really have gotten to the bottom of the problem. I spoke with a premium manufacturer of such slides and they seemed to indicate that it is a problem with the coating, but couldn't tell me for sure. All I know is that certain brands do this more than others. If you know what I mean, you know it is a problem. My bath is pure distilled H2O with no gelatin or Sta-on added. It is if the adhesive properties are SO good that they will not release the water when vertically drained and have to be shaken off or cut with a razor blade at bottom to release the water. Anyway, if anyone has an insight or two on this, I would be interested. It seem sthe most challenging issues are ones that seem related to some of the most simple tasks that one has performed for many years!! Manufacturers understand what I mean, but cannot pinpoint the problem for me via phone or e-mail. Anyone see this and have a chemical/mechanical solution they have developed over the years? Thanks! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://*lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Water collecting at bottom of sections
Am I the only person who paraffin sections by: cutting the sections, grabbing the ribbon with forceps or paintbrush, placing it on a dry slide, adding water underneath the ribbon with a pastuer pipet, and then placing the slide on a slide warmer. The water will heat up and the paraffin ribbon will expand on the bubble of water on the slide.Leave the slide on a slide warmer overnight and the water is evaporates. Some people blot off the extra water. Maybe that's just not any easier than picking up the sections from a water bath. Emily -- Outside of a dog, a book is man's best friend. Inside of a dog it's too dark to read. --Groucho Marx ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] artifacts in sections
I have always soaked my biopsies on ice water. It always seems to help. Sent from my Verizon Wireless BlackBerry -Original Message- From: histonet-requ...@lists.utsouthwestern.edu Sender: histonet-boun...@lists.utsouthwestern.edu Date: Fri, 23 Jul 2010 13:01:46 To: histonet@lists.utsouthwestern.edu Reply-To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 80, Issue 27 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than Re: Contents of Histonet digest... Today's Topics: 1. Laboratory Labeling Systems... (Barone, Carol ) 2. Re: Histonet Digest, Vol 80, Issue 26 (Stephanie Rodriguez) 3. Histology metrics (Scherer, Kris) 4. RE: HPV testing (McMahon, Loralee A) 5. RE: Biocare P63 with Dako Flex detection (Cynthia Pyse) 6. biohazard bags (Jeff Birkner) 7. RE: biohazard bags (Stacy McLaughlin) 8. Re: biohazard bags (dkb...@chs.net) 9. Sectioning problem (kgrob...@rci.rutgers.edu) 10. Re: Sectioning problem (Bryan Llewellyn) 11. Staining dish for vertical 30-slide rack (Morken, Tim) 12. RE: biohazard bags (Rathborne, Toni) 13. p-TBK-1 Ab (Michelle Mathews) 14. Artifacts in histology section (Aazath Raj) 15. Microwave Decalcification (Figliuolo, Leticia) 16. Re: Artifacts in histology section (Rene J Buesa) 17. artifacts of sectioning (Tench, Bill) 18. Formalin substitutes (Weaver, Colin) 19. RE: artifacts of sectioning (Matt Brooks) 20. HPV (rick.garnh...@memorialhealthsystem.com) -- Message: 1 Date: Thu, 22 Jul 2010 13:21:26 -0400 From: Barone, Carol cbar...@nemours.org Subject: [Histonet] Laboratory Labeling Systems... To: histonet@lists.utsouthwestern.edu Message-ID: 37e4bac017f57141af64faa5aeb04ce8033a7...@wlmmsx01.nemours.org Content-Type: text/plain; charset=us-ascii HistonettersWhat are your thoughts: We are getting ready to purchase a labeling system for our Core Facility. We have limited $'s and have demo'ed three systems. We have many different projects,with varying numbers of samples, blocks and slides. We would like to cover the widest amount of needs, with the systemnot just cassettes and slides, but samples in freezers to antibodies we have alloquotted. We are about to purchase a two-D bar-coding system that can label everything we need and work with our present EXCEL data bases. We are leaning toward a Brady System, where we can purchase individually the parts we need for labeling cassettes to eppy's and we especially like that they... not only have a label that can be attached to frozen vials... but that there are several types of scanners available for the different stations in our lab. This system meets our CER requirements and is up-gradable for adding more stations if needed at alater time. Sounds too good to be trueso that is where you all come in. Is anyone using a Brady Labeling System? Good? Bad? Problems? What are your thoughts? Thanks for the impromptu survey. We plan to order in August, if there are no big issues, with those that use it. We demo's the labels and are happy with them. We are going alpha numeric/2-D...3 lines of text, is needed for our research work. Thanks for your thoughts. C. Barone Director Histotechnology Core Lab cbar...@nemours.org -- Message: 2 Date: Thu, 22 Jul 2010 10:44:10 -0700 From: Stephanie Rodriguez srodrig...@phenopath.com Subject: [Histonet] Re: Histonet Digest, Vol 80, Issue 26 To: histonet@lists.utsouthwestern.edu Message-ID: c86dd17a.d08%srodrig...@phenopath.com Content-Type: text/plain; charset=US-ASCII As do we. Stephanie Rodriguez, HTL(ASCP), QIHC Senior Molecular Technologist-FISH IHC Technologist III Phenopath Laboratories Seattle, WA (206) 374-9000 On 7/22/10 10:04 AM, histonet-requ...@lists.utsouthwestern.edu histonet-requ...@lists.utsouthwestern.edu wrote: Message: 4 Date: Thu, 22 Jul 2010 08:18:27 -0700 From: Tench, Bill bill.te...@pph.org Subject: [Histonet] hpv testing on head and neck ca's To: histonet@lists.utsouthwestern.edu Message-ID: 2820431bf953bb4da3e9e1a5882265fd034a5...@mail1.pph.local Content-Type: text/plain; charset=us-ascii We do p16 on squamous ca of the head and neck region routinely Bill Tench Associate Dir. Laboratory Services Chief, Cytology Services Palomar Medical Center 555 E. Valley Parkway Escondido, California 92025 bill.te...@pph.org Voice: 760- 739-3037 Fax: 760-739-2604 This e-mail message, including any attachments, is for the sole use of the
[Histonet] Buffers
Always, Always, Always pH your buffers when you make them and adjust them properly. If you do not you will NEVER know if your buffer is really at the pH you think it is. You can't pour out 10 liters of water exactly the same every time. You can't be sure the factory weighed the salts perfectly every time. It is just a bad idea to assume these things are right and blame variations on the pH of the water expecting unbuffered water to be a constant. Ok, getting off the soapbox, Amos ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
