RE: [Histonet] Eosin to dye small Biopsies
We use about the same amount but in our last 100% Alc. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histot...@imagesbyhopper.com Sent: Thursday, October 21, 2010 4:40 PM To: Scott, Allison D Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Eosin to dye small Biopsies We use about 40ml of eosin in the first 100% alcohol in both of our large specimen small biopsy machines. Michelle On Oct 21, 2010, at 4:33 PM, Scott, Allison D allison_sc...@hchd.tmc.edu wrote: Hello to all in histoland. Are any of you using eosin on the processor to dye your small bx's? If so, are you putting it in the 100% alcohol to do so? Any help in this matter will be greatly appreciated. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital Houston, Texas 77026 CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 (HIPAA), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Endothelial histochemistry
Hi, I am just new in this group, so I hope this is the way I can get help. I am doing histochemistry on human hypothalamus and I need to stain endothelial cells. I tried UEA-I (lectin) and I got microvessels stained as the whole and like a line. But I would like to have an image of the endothelial cells of even larger vessels, the way that I can show cell by cell in a circular shape of a vessel. Could you help me and tell me what antibody or lectin helps me best. Regards Ali ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Eosin to dye small Biopsies
We use cobalt blue as it does not interfere with FISH Sent from my Verizon Wireless Phone - Reply message - From: susan.wal...@hcahealthcare.com Date: Fri, Oct 22, 2010 3:28 am Subject: [Histonet] Eosin to dye small Biopsies To: histot...@imagesbyhopper.com, allison_sc...@hchd.tmc.edu Cc: histonet@lists.utsouthwestern.edu We use about the same amount but in our last 100% Alc. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histot...@imagesbyhopper.com Sent: Thursday, October 21, 2010 4:40 PM To: Scott, Allison D Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Eosin to dye small Biopsies We use about 40ml of eosin in the first 100% alcohol in both of our large specimen small biopsy machines. Michelle On Oct 21, 2010, at 4:33 PM, Scott, Allison D allison_sc...@hchd.tmc.edu wrote: Hello to all in histoland. Are any of you using eosin on the processor to dye your small bx's? If so, are you putting it in the 100% alcohol to do so? Any help in this matter will be greatly appreciated. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital Houston, Texas 77026 CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 (HIPAA), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Eosin to dye small Biopsies
We use eosin at the grossing bench on endoscopic specimens and hematoxylin on prostate/liver biopsies. The pathologist drops a drop of stain on each specimen. Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical Center I 200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F: 804-765-5582 l dkb...@chs.net Scott, Allison D allison_sc...@hchd.tmc.edu Sent by: histonet-boun...@lists.utsouthwestern.edu 10/21/2010 04:37 PM To histonet@lists.utsouthwestern.edu cc Subject [Histonet] Eosin to dye small Biopsies Hello to all in histoland. Are any of you using eosin on the processor to dye your small bx's? If so, are you putting it in the 100% alcohol to do so? Any help in this matter will be greatly appreciated. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital Houston, Texas 77026 CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 (HIPAA), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] pituitary stain
--- dear all can you share me pituitary stain protocol called PAS/ celestine blue / Orange G? thanks in advance mohamed ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] pituitary stain
Mohamed, The University of Nottingham Histology department has an extensive website of histology protocols: http://www.nottingham.ac.uk/pathology/default.html There is a protocol for PAS/OG on that site. I do not have any experience using it, but as it comes out of John Bancroft's old lab it will undoubtedly be very sound. It does differ somewhat from the method of AGE Pearse that I followed many, many moons ago in Glasgow. We also always performed an OFG (orange-fuchsin-green) method on all pituitary bxs (if you need a copy of that let me know).As I'm sure you are aware, tinctorial stains for pituitary adenomas have been replaced with immunohistochemistry for the hormones ACTH, FSH, GH, LH, prolactin and TSH: PAS/OG 1. Section to 70% alcohol 2. leave in 70% alcohol for 5 minutes 3. transfer to Solution A for 15 minutes at room temp 4. Wash in 70% alcohol for 5 minutes 5. transfer to reducing rinse B for 5 minutes 6. 70% alcohol for 3 minutes 7. rinse in running tap water 8. Schiff's reagent 20-30 minutes 9. wash in water 3 minutes 10. Celestin Blue solution 10 minutes 11. Rinse well in water 12. Hematoxylin 10 minutes 13. wash in water 14. 2% Orange G in 5% phosphotungstic acid for 3 minutes 15. wash in water 16. dehydrate, clear and mount Result Nuclei - black RBC's - yellow-orange Alpha granules - orange Beta granules - magenta (PAS +ve) Solution A Periodic acid - 400mg Distilled water - 15 ml 100% alcohol - 35 ml Reducing Solution B Sodium thiosulphate - 1gm Distilled water - 20ml 100% alcohol- 30ml HCl - 0.5ml Rationale PAS converts 1,2 glycols to aldehyde. The reducing step is thought to block the subsequent reaction of collagen and retic fibers with the Schiff's reagent. Beta granules contain muco- or glycoproteins that react with Schiff's to give their magenta staining. Orange G, which is an acidic dye, stains the alpha granules. As RBC's are also acidophilic in nature, they also pick up the orange coloration. Reference Pearse AGE. The cytochemical demonstration of gonadotrophic hormone in the human anterior hypophysis. J Pathol Bacteriol. 1949: 61; 195-202 If you need a recipe for the celestin blue solution, drop me a line and I'll get it to you, but you should find it in most good histotechnology text-books, or on-line Best wishes Ronnie Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager ChildLab, a Division of Nationwide Children's Hospital www.childlab.com 700 Children's Drive Columbus, OH 43205 (P) 614-722-5450 (F) 614-722-2899 ronald.hous...@nationwidechildrens.orgmailto:ronald.hous...@nationwidechildrens.org www.NationwideChildrens.orghttp://www.NationwideChildrens.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of mohamed abd el razik Sent: Friday, October 22, 2010 8:36 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] pituitary stain --- dear all can you share me pituitary stain protocol called PAS/ celestine blue / Orange G? thanks in advance mohamed ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet - Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Need opinions on HE stainers please!
Our laboratory will be purchasing new HE stain equipment in the near future. We would like to hear the pros and cons out there for the Thermo-Shandon Gemini, Sakura Prisma, Leica Autostainer XL and the Ventana Symphony. Thanks in advance for your input. Kendall A. Neely Histology Technical Specialist Shands Rocky Point Laboratories (352) 265-0111, x72113 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Eosin to dye small Biopsies
Allison Scott at LBJ Hospital in Houston, Texas asks about the use of eosin to dye small biopsy specimens. Several replies mention addition of eosin to one of the processing alcohols. I have never seen this done, in maybe 60 pathology services I've worked in. (I'd know, because I nearly always examine the paraffin block when I order recuts or send a case out for consultation.) It's a fine time-waster for the pathologist to mark small specimens with dye while grossing. I've used Mercurochrome (merbromin, related to eosin but with 26% mercury) which fortunately was banned in the USA about ten years ago. I've used eosin, and I've used safranin (from the microbiology lab's Gram stain setup). I don't know whether safranin interferes with FISH, as eosin is well known to, nor do I know if you can put safranin in the processing alcohol. And I've used Davidson tissue marking inks. I've never seen or heard of cobalt blue used for this purpose - is this the insoluble coloring material, chemically cobalt aluminate? Bob Richmond Samurai Pathologist Knoxville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] eosin
I think the use in a processor becomes a local culture. I think everyone in my community does it (someone spread the word), so now we all do. I believe that we are all putting it in the last alcohol. It really makes facing a block on minute pieces a whole lot easier. We found marking the tissue directly tedious, and it didn't persist as well in the processing. Because there is often so little visible material in our FNA cell blocks, we mark the histogel pellet with Davidson ink, and when the tech has just faced off the ink, he/she knows its time to start collecting sections. Bill Tench Associate Dir. Laboratory Services Chief, Cytology Services Palomar Medical Center 555 E. Valley Parkway Escondido, California 92025 bill.te...@pph.org Voice: 760- 739-3037 Fax: 760-739-2604 [None] made the following annotations - Confidential E-Mail: This e-mail is intended only for the person or entity to which it is addressed, and may contain information that is privileged, confidential, or otherwise protected from disclosure. Dissemination, distribution, or copying of this e-mail or the information herein by anyone other than the intended recipient is prohibited. If you have received this e-mail in error, please notify the sender by reply e-mail, and destroy the original message and all copies. - ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Endothelial histochemistry
Hi Ali, Although I do not ever perform endothelial immunohistochemistry in our laboratory, I know it is common to use an antibody against CD31 to selectively stain endothelial cells and this may work well in pituitary tissue. Hope that helps, Kristen Ali Nasr Esfahani alina...@student.liu.se 10/22/10 5:31 AM Hi, I am just new in this group, so I hope this is the way I can get help. I am doing histochemistry on human hypothalamus and I need to stain endothelial cells. I tried UEA-I (lectin) and I got microvessels stained as the whole and like a line. But I would like to have an image of the endothelial cells of even larger vessels, the way that I can show cell by cell in a circular shape of a vessel. Could you help me and tell me what antibody or lectin helps me best. Regards Ali ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Re: Eosin to dye small Biopsies
As much as I respect Dr. Richmond, I would have to disagree that staining bx's with eosin is a waste of pathologist time. It helps the embedding tech and cutting tech see the minute pieces, which may be otherwise lost. Sometimes that is the diagnostic material. We would not want to put a patient through another procedure because we couldn't recover the tissue submitted. We use a vial with a dropper. Once the biopsy is placed in the cassette you take 1 second more to drop a drop of eosin on the specimen. Well worth everyone's time in my humble opinion. Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical Center I 200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F: 804-765-5582 l dkb...@chs.net Robert Richmond rsrichm...@gmail.com Sent by: histonet-boun...@lists.utsouthwestern.edu 10/22/2010 10:44 AM To histonet@lists.utsouthwestern.edu cc Subject [Histonet] Re: Eosin to dye small Biopsies Allison Scott at LBJ Hospital in Houston, Texas asks about the use of eosin to dye small biopsy specimens. Several replies mention addition of eosin to one of the processing alcohols. I have never seen this done, in maybe 60 pathology services I've worked in. (I'd know, because I nearly always examine the paraffin block when I order recuts or send a case out for consultation.) It's a fine time-waster for the pathologist to mark small specimens with dye while grossing. I've used Mercurochrome (merbromin, related to eosin but with 26% mercury) which fortunately was banned in the USA about ten years ago. I've used eosin, and I've used safranin (from the microbiology lab's Gram stain setup). I don't know whether safranin interferes with FISH, as eosin is well known to, nor do I know if you can put safranin in the processing alcohol. And I've used Davidson tissue marking inks. I've never seen or heard of cobalt blue used for this purpose - is this the insoluble coloring material, chemically cobalt aluminate? Bob Richmond Samurai Pathologist Knoxville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Need opinions on HE stainers please!
For the love of your sanity (and keeping your pathologists happy)= DO
NOT BUY A SYMPHONY Their reagents are all proprietary and so
troubleshooting is almost impossible without paying Ventana to have
tech he= lp loom around your lab. The slides take 3 days or so to dry
before y= ou can file them, so you have to have tons of counterspace
(depending on yo= ur volume) to let them sit there in slide folders
before you can file. = ; The pathologists I worked with when we were
trying this thing out hated t= he staining and said that it wasn't
consistent from slide to slide. N= ot to mention the physical size of
the thing and the reagents are insane ex= pensive!!!
Just my opinion =)
Sarah Goebel, B.A., H= T (ASCP)
Histotechnician
XBiotech USA Inc.
8201 East Riverside Dr. Bldg 4 Suite 100
= em
Austin, Te= xas 78744
(512)386-2907
Original Message
Subject: [Histonet] Need opinions on HE stainers please!
From: Kendall Neely [1]nee...@s= hands.ufl.edu
Date: Fri, October 22, 2010 7:31 am
To: [2]histo...@lists= .utsouthwestern.edu
Our laboratory will be purchasing new HE stain equipment in the near
f= uture. We would like to hear the pros and cons out there for the
Thermo-Sh= andon Gemini, Sakura Prisma, Leica Autostainer XL and the
Ventana Symphony.= Thanks in advance for your input.
Kendall A. Neely
Histology Technical Specialist
Shands Rocky Point Laboratories
(352) 265-0111, x72113
___
Histonet mailing list
[3]histo...@lists.utsouth= western.edu
[4]http:= //lists.utsouthwestern.edu/mailman/listinfo/histonet
References
1. 3Dmailto:nee...@shands.ufl.edu;
2. 3Dmailto:histonet@lists.utsouthwestern.edu;
3. 3Dmailto:Histonet@lists.utsouthwestern.edu;
4. 3Dhttp://lists.utsouthwestern.edu/mailman/listinfo/histonet;
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Re: [Histonet] eosin
It sounds like I am in the minority in pitting the eosin in the *first* 100% alcohol! I agree with Bill, it really makes a HUGE difference in small, minute tissues. We did run into one issue though, we were using orange biopsy cassettes and the red-orange tissue was difficult to spot in the orange cassettes! We have since switched to an aqua color and no more hiding specimens! ;o) Michelle On Oct 22, 2010, at 10:51 AM, Tench, Bill bill.te...@pph.org wrote: I think the use in a processor becomes a local culture. I think everyone in my community does it (someone spread the word), so now we all do. I believe that we are all putting it in the last alcohol. It really makes facing a block on minute pieces a whole lot easier. We found marking the tissue directly tedious, and it didn't persist as well in the processing. Because there is often so little visible material in our FNA cell blocks, we mark the histogel pellet with Davidson ink, and when the tech has just faced off the ink, he/she knows its time to start collecting sections. Bill Tench Associate Dir. Laboratory Services Chief, Cytology Services Palomar Medical Center 555 E. Valley Parkway Escondido, California 92025 bill.te...@pph.org Voice: 760- 739-3037 Fax: 760-739-2604 [None] made the following annotations - Confidential E-Mail: This e-mail is intended only for the person or entity to which it is addressed, and may contain information that is privileged, confidential, or otherwise protected from disclosure. Dissemination, distribution, or copying of this e-mail or the information herein by anyone other than the intended recipient is prohibited. If you have received this e-mail in error, please notify the sender by reply e-mail, and destroy the original message and all copies. - ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] eosin
We put eosin in the first 100% alcohol. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histot...@imagesbyhopper.com Sent: Friday, October 22, 2010 11:18 AM To: Tench, Bill Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] eosin It sounds like I am in the minority in pitting the eosin in the *first* 100% alcohol! I agree with Bill, it really makes a HUGE difference in small, minute tissues. We did run into one issue though, we were using orange biopsy cassettes and the red-orange tissue was difficult to spot in the orange cassettes! We have since switched to an aqua color and no more hiding specimens! ;o) Michelle On Oct 22, 2010, at 10:51 AM, Tench, Bill bill.te...@pph.org wrote: I think the use in a processor becomes a local culture. I think everyone in my community does it (someone spread the word), so now we all do. I believe that we are all putting it in the last alcohol. It really makes facing a block on minute pieces a whole lot easier. We found marking the tissue directly tedious, and it didn't persist as well in the processing. Because there is often so little visible material in our FNA cell blocks, we mark the histogel pellet with Davidson ink, and when the tech has just faced off the ink, he/she knows its time to start collecting sections. Bill Tench Associate Dir. Laboratory Services Chief, Cytology Services Palomar Medical Center 555 E. Valley Parkway Escondido, California 92025 bill.te...@pph.org Voice: 760- 739-3037 Fax: 760-739-2604 [None] made the following annotations - Confidential E-Mail: This e-mail is intended only for the person or entity to which it is addressed, and may contain information that is privileged, confidential, or otherwise protected from disclosure. Dissemination, distribution, or copying of this e-mail or the information herein by anyone other than the intended recipient is prohibited. If you have received this e-mail in error, please notify the sender by reply e-mail, and destroy the original message and all copies. - ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Need opinions on HE stainers please!
We have a (refurbished) Lecia Autostainer XL and really like it. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kendall Neely Sent: Friday, October 22, 2010 10:32 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Need opinions on HE stainers please! Our laboratory will be purchasing new HE stain equipment in the near future. We would like to hear the pros and cons out there for the Thermo-Shandon Gemini, Sakura Prisma, Leica Autostainer XL and the Ventana Symphony. Thanks in advance for your input. Kendall A. Neely Histology Technical Specialist Shands Rocky Point Laboratories (352) 265-0111, x72113 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] eosin
We add 1.0 mls of 10% aqueous Eosin to the first 100% Ethanol; we will then have Eosin in all of the Ethanol containers after they have been rotated. This removed the time consuming task of dotting each tissue at grossing and allows for easy visualization at embedding and sectioning. I have not had a complaint about the eosin interfering with FISH from our referral lab. Cathy Fitzpatrick Anatomic Pathology Section Head Kelowna General Hospital _ From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histot...@imagesbyhopper.com Sent: Friday, October 22, 2010 8:18 AM To: Tench, Bill Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] eosin It sounds like I am in the minority in pitting the eosin in the *first* 100% alcohol! I agree with Bill, it really makes a HUGE difference in small, minute tissues. We did run into one issue though, we were using orange biopsy cassettes and the red-orange tissue was difficult to spot in the orange cassettes! We have since switched to an aqua color and no more hiding specimens! ;o) Michelle On Oct 22, 2010, at 10:51 AM, Tench, Bill bill.te...@pph.org wrote: I think the use in a processor becomes a local culture. I think everyone in my community does it (someone spread the word), so now we all do. I believe that we are all putting it in the last alcohol. It really makes facing a block on minute pieces a whole lot easier. We found marking the tissue directly tedious, and it didn't persist as well in the processing. Because there is often so little visible material in our FNA cell blocks, we mark the histogel pellet with Davidson ink, and when the tech has just faced off the ink, he/she knows its time to start collecting sections. Bill Tench Associate Dir. Laboratory Services Chief, Cytology Services Palomar Medical Center 555 E. Valley Parkway Escondido, California 92025 bill.te...@pph.org Voice: 760- 739-3037 Fax: 760-739-2604 [None] made the following annotations - Confidential E-Mail: This e-mail is intended only for the person or entity to which it is addressed, and may contain information that is privileged, confidential, or otherwise protected from disclosure. Dissemination, distribution, or copying of this e-mail or the information herein by anyone other than the intended recipient is prohibited. If you have received this e-mail in error, please notify the sender by reply e-mail, and destroy the original message and all copies. - ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _ No virus found in this message. Checked by AVG - www.avg.com Version: 10.0.1136 / Virus Database: 422/3212 - Release Date: 10/22/10 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Need opinions on HE stainers please!
We have two leica XL Stainers and love them. We don't have the unit to transfer to the coverslippers yet and that would be great but minor overall. We are just setting up some special stains on it and the run up- run down programs for special stains. Pam Marcum UAMS - Original Message - From: Margaret Sherwood msherw...@partners.org To: Kendall Neely nee...@shands.ufl.edu, histonet@lists.utsouthwestern.edu Sent: Friday, October 22, 2010 10:44:50 AM Subject: RE: [Histonet] Need opinions on HE stainers please! We have a (refurbished) Lecia Autostainer XL and really like it. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kendall Neely Sent: Friday, October 22, 2010 10:32 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Need opinions on HE stainers please! Our laboratory will be purchasing new HE stain equipment in the near future. We would like to hear the pros and cons out there for the Thermo-Shandon Gemini, Sakura Prisma, Leica Autostainer XL and the Ventana Symphony. Thanks in advance for your input. Kendall A. Neely Histology Technical Specialist Shands Rocky Point Laboratories (352) 265-0111, x72113 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet - Original Message - From: Margaret Sherwood msherw...@partners.org To: Kendall Neely nee...@shands.ufl.edu, histonet@lists.utsouthwestern.edu Sent: Friday, October 22, 2010 10:44:50 AM Subject: RE: [Histonet] Need opinions on HE stainers please! We have a (refurbished) Lecia Autostainer XL and really like it. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kendall Neely Sent: Friday, October 22, 2010 10:32 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Need opinions on HE stainers please! Our laboratory will be purchasing new HE stain equipment in the near future. We would like to hear the pros and cons out there for the Thermo-Shandon Gemini, Sakura Prisma, Leica Autostainer XL and the Ventana Symphony. Thanks in advance for your input. Kendall A. Neely Histology Technical Specialist Shands Rocky Point Laboratories (352) 265-0111, x72113 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Need opinions on HE stainers please!
We are using the Sakura Prisma with attached coverslipper; it is a workhorse and allows for the use of what ever reagents/stains that you prefer. We are running our HE and H Pylori stains simultaneously. We experienced a small problem with the leveling of the instrument at the initial set up but once that was corrected it has been working well since then. The only draw back that I can see with the attached coverslipper is that the slides take longer to dry than with the older model. The slides are kept horizontal rather than vertical so the xylene doesn't run off. Cathy Fitzpatrick Anatomic Pathology Section Head Kelowna General Hospital _ From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kendall Neely Sent: Friday, October 22, 2010 7:32 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Need opinions on HE stainers please! Our laboratory will be purchasing new HE stain equipment in the near future. We would like to hear the pros and cons out there for the Thermo-Shandon Gemini, Sakura Prisma, Leica Autostainer XL and the Ventana Symphony. Thanks in advance for your input. Kendall A. Neely Histology Technical Specialist Shands Rocky Point Laboratories (352) 265-0111, x72113 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _ No virus found in this message. Checked by AVG - www.avg.com Version: 10.0.1136 / Virus Database: 422/3212 - Release Date: 10/22/10 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Endothelial histochemistry
Hi Ali, I tried FITC cd31 on mouse liver frozen section with tumors it worked really well. Good luck, Anil Kumar. On 22/10/10 11:29, Ali Nasr Esfahani alina...@student.liu.se wrote: Hi, I am just new in this group, so I hope this is the way I can get help. I am doing histochemistry on human hypothalamus and I need to stain endothelial cells. I tried UEA-I (lectin) and I got microvessels stained as the whole and like a line. But I would like to have an image of the endothelial cells of even larger vessels, the way that I can show cell by cell in a circular shape of a vessel. Could you help me and tell me what antibody or lectin helps me best. Regards Ali ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Processors
Greetings Histologist, Does anyone have positive or negative comments on the Thermo Citadel 1000 ? Is there an abundance of fumes during processing? Thanks again, Karen Karen E. Cruise Histologist / Research Technician II Washington University School of Medicine Laboratory for Translational Pathology 216 S. Kingshighway Rm #2332 St Louis, MO 63110 314-454-8636 Office 314-454-5525 Fax kcru...@path.wustl.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Microtome for undecalcified tissues
Hi, I'm looking for a good used sledge or rotary microtome capable of cutting MMA emebdded bone specimens. Can anyone reccomend a good model, manufacturer, supplier or know of a good used machine ? Many thanks. Adam ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Histo Opening in MA
MPath Search Partners is looking for a Histotechnician/Histotechnologist candidate for permanent direct hire in Massachusetts. *Shift/Schedule:* Monday-Friday, Day Shift Hours * * *Position: *Histotechnician/Histotechnologist *Location: * Pharmaceutical Company in the Burlington, MA area *Requirements:* HT/HTL ASCP preferred Degree preferred Must have experience with working with Animal Tissue *Benefits:* Generous PTO accrual schedule 401k Competitive wages/benefits Paid training Health Benefits *Application:* To be considered please submit your resume in order to schedule a phone screen with one of our recruiters. Please be aware that all resumes submitted to Allied Search Partners are confidential. *Serious inquiries only please.* 1. No resume will be submitted until we speak to you for a phone screen. 2. No resume will be submitted to client if you are currently or previously worked for client. 3. Name, and contact information will not be given out without your consent. Please submit resume to aly...@alliedsearchpartners.com -- Alyssa Peterson, Director of Candidate Recruitment LinkedIN:http://www.linkedin.com/in/alyssapetersonasp Allied Search Partners T: 888.388.7571 F: 888.388.7572 www.alliedsearchpartners.com This email including its attachments is intended only for the confidential use of the individual to whom it is addressed. If you are not the intended recipient, any use, dissemination, distribution or copying of this message or its attachments is prohibited. If you have received this message in error, please notify us immediately, and delete this message and its attachments permanently from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Microtome for undecalcified tissues
Depending on the size of the bone the Leica 2255 is a great mictorome to use with tungsten carbide blades. It is heavy enough to larger blocks and is motorized. If it is not motorized I would not try it for as that will give you the best results. Pam Marcum - Original Message - From: Adam Hacking ahack...@yahoo.com To: Histonet@lists.utsouthwestern.edu Sent: Friday, October 22, 2010 11:42:52 AM Subject: [Histonet] Microtome for undecalcified tissues Hi, I'm looking for a good used sledge or rotary microtome capable of cutting MMA emebdded bone specimens. Can anyone reccomend a good model, manufacturer, supplier or know of a good used machine ? Many thanks. Adam ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet - Original Message - From: Adam Hacking ahack...@yahoo.com To: Histonet@lists.utsouthwestern.edu Sent: Friday, October 22, 2010 11:42:52 AM Subject: [Histonet] Microtome for undecalcified tissues Hi, I'm looking for a good used sledge or rotary microtome capable of cutting MMA emebdded bone specimens. Can anyone reccomend a good model, manufacturer, supplier or know of a good used machine ? Many thanks. Adam ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] I
I will be out of the office starting 10/21/2010 and will not return until 10/25/2010. In my absence please ask for Mary Campbell . If this is urgent or you need to speak to me directly you can contact me on my cell phone number 858-472-4266. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Histotech Opening in the Midwest
MPath Search Partners is looking for a Histotechncian/Histotechnologist candidate for permanent direct hire in Wisconsin. *Shift/Schedule:* Monday-Friday, Day Shift Hours, 38 hours per week * * *Position: *Histotechnician/Histotechnologist *Location: * State of the art laboratory in Manitowoc, Wisconsin 10 Dermatology offices throughout WI One centralized physician office laboratory *Requirements:* HT/HTL ASCP preferred Degree preferred Mohs experience *Benefits:* * Generous PTO accrual schedule * 401k match profit sharing * Competitive wages/benefits * Paid holidays * Paid training *Application:* To be considered please submit your resume in order to schedule a phone screen with one of our recruiters. Please be aware that all resumes submitted to Allied Search Partners are confidential. *Serious inquiries only please.* 1. No resume will be submitted until we speak to you for a phone screen. 2. No resume will be submitted to client if you are currently or previously worked for client. 3. Name, and contact information will not be given out without your consent. Please submit resume to aly...@alliedsearchpartners.com -- Alyssa Peterson, Director of Candidate Recruitment LinkedIN:http://www.linkedin.com/in/alyssapetersonasp Allied Search Partners T: 888.388.7571 F: 888.388.7572 www.alliedsearchpartners.com This email including its attachments is intended only for the confidential use of the individual to whom it is addressed. If you are not the intended recipient, any use, dissemination, distribution or copying of this message or its attachments is prohibited. If you have received this message in error, please notify us immediately, and delete this message and its attachments permanently from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Re: Eosin to dye small Biopsies
We have our grossing staff cover all GI biopsies, breast biopsies and endometrial curettings or any small colorless tissue with 0.1% basic fuchsin. It leaves it a nice pinkish tint after processing, the specimens are visible in the paraffin, and we haven't had any pathologist complaints. On Fri, Oct 22, 2010 at 8:10 AM, dkb...@chs.net wrote: As much as I respect Dr. Richmond, I would have to disagree that staining bx's with eosin is a waste of pathologist time. It helps the embedding tech and cutting tech see the minute pieces, which may be otherwise lost. Sometimes that is the diagnostic material. We would not want to put a patient through another procedure because we couldn't recover the tissue submitted. We use a vial with a dropper. Once the biopsy is placed in the cassette you take 1 second more to drop a drop of eosin on the specimen. Well worth everyone's time in my humble opinion. Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical Center I 200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F: 804-765-5582 l dkb...@chs.net Robert Richmond rsrichm...@gmail.com Sent by: histonet-boun...@lists.utsouthwestern.edu 10/22/2010 10:44 AM To histonet@lists.utsouthwestern.edu cc Subject [Histonet] Re: Eosin to dye small Biopsies Allison Scott at LBJ Hospital in Houston, Texas asks about the use of eosin to dye small biopsy specimens. Several replies mention addition of eosin to one of the processing alcohols. I have never seen this done, in maybe 60 pathology services I've worked in. (I'd know, because I nearly always examine the paraffin block when I order recuts or send a case out for consultation.) It's a fine time-waster for the pathologist to mark small specimens with dye while grossing. I've used Mercurochrome (merbromin, related to eosin but with 26% mercury) which fortunately was banned in the USA about ten years ago. I've used eosin, and I've used safranin (from the microbiology lab's Gram stain setup). I don't know whether safranin interferes with FISH, as eosin is well known to, nor do I know if you can put safranin in the processing alcohol. And I've used Davidson tissue marking inks. I've never seen or heard of cobalt blue used for this purpose - is this the insoluble coloring material, chemically cobalt aluminate? Bob Richmond Samurai Pathologist Knoxville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Patrick Laurie HT(ASCP)QIHC CellNetix Pathology Laboratories 1124 Columbia Street, Suite 200 Seattle, WA 98104 plau...@cellnetix.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Clarification-interfacing the IHC bond and Cassettelabelers to Co-Path
We have interfaced our Bonds with Soft Path LIS system. My justification for this started with using it for LEAN processes in that the orders for IHC went directly from the Pathologist to the Bond and eliminated the need for my techs to have to input individual orders for IHC by hand. Since we have set up our slide labelers to be recognized as just another printer as far as the LIS is concerned, we do not use paper labels at all but have 2d barcodes printed directly on our slides. When an order is put in by the pathologist for IHC, my techs can see the order, cut the section and print the slide with the correct bar code. Bond recognizes the barcode and initializes the tests that have been ordered and transferred from the pathologist. This has accomplished the following: No tech time lost in printing labels for slides to go on the bond. No ambiguity or lost IHC orders due to hand writing orders by the pathologist. No chance of keystroke errors on the part of my IHC tech while putting manual orders into the Bond. In addition to eliminating hand writing and manual keystrokes, which are distinct patient safety issue, I have calculated that having the interface has saved me approximately 0.7 FTE. Instead of having to hire extra staff to cover increased workloads or wasting existing staff on extraneous tasks (hand labeling, manually entering orders, etc), I can utilize them in other areas. The patient safety aspect of eliminating extra tasks involving manual data entry is huge. A majority of the lawsuits against pathology labs involve some aspect of human error resulting from manual tasks in labeling or data entry. In addition to being able to market my lab as patient safety focused, we have eliminated a major source of potential lawsuits. It's hard to put a price tag on what that saves other than to say that the costs are sometimes much more than the dollar figures paid out. Steve -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Akemi Allison Sent: Thursday, October 21, 2010 4:01 PM To: Walter Benton Cc: Histonet Subject: Re: [Histonet] Clarification-interfacing the IHC bond and Cassettelabelers to Co-Path Hi Walter and Histo-subscribers, Ist I want to thank Walter for his quick reply. I appreciate your answer! 2nd, I appreciate any and all replies, but does anyone have an article that addresses issues that can occur such as: Efficiency Omitting Duplication of Tests ordered: Additional Slides, Special Stains, IHC, FISH, CISH, Cost effectiveness due to omission of errors Patient Safety Thanks Akemi Allison BS, HT (ASCP) HTL Director Phoenix Lab Consulting Tele: 408.335.9994 E-Mail: akemiat3...@yahoo.com On Oct 21, 2010, at 11:38 AM, Walter Benton wrote: Efficiency Patient Safety Orders for the Bond come directly from the LIS and can not be misunderstood due to poor handwriting, since they are interfaced with the LIS. Walter Benton HT(ASCP)QIHC Histology Supervisor Chesapeake Urology Associates 806 Landmark Drive, Suite 126 (All Deliveries to Suite 127) Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) wben...@cua.md From: histonet-boun...@lists.utsouthwestern.edu [histonet- boun...@lists.utsouthwestern.edu] On Behalf Of Akemi Allison [akemiat3...@yahoo.com] Sent: Thursday, October 21, 2010 2:33 PM To: Histonet Subject: [Histonet] interfacing the IHC bond and Cassette labelers to Co-Path Hi out there in Histo Land! I would like your assistance in answering a question that was proposed by a friend who is not a histonet member. I don't have the answer, but know that one of you would. Below is the question: Could you help me justify the importance of interfacing our IHC bond and Cassette labelers to Co-Path? A simple paragraph, or if you have, a white paper, that would be great. I am attempting to get the interfaces approved through our IT Department and running up against some roadblocks. Thank you in advance for your assistance, Akemi Allison BS, HT (ASCP) HTL Director Phoenix Lab Consulting Tele: 408.335.9994 E-Mail: akemiat3...@yahoo.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] formalin specimen containers
Does anyone know of or have access to changes in regulations for formalin specimen containers? At our recent CAP inspection, we were told that JCAHO and OSHA were mandating that formalin specimen containers should now have locking lids. This is the first I have heard of this. Thanks Ronnie Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager ChildLab, a Division of Nationwide Children's Hospital www.childlab.com 700 Children's Drive Columbus, OH 43205 (P) 614-722-5450 (F) 614-722-2899 ronald.hous...@nationwidechildrens.orgmailto:ronald.hous...@nationwidechildrens.org www.NationwideChildrens.orghttp://www.NationwideChildrens.org One person with passion is better than forty people merely interested. ~ E.M. Forster - Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Cheap slide scanner for rat brains?
Hello Everyone, Does anyone have a good way to get low magnification images from slides? I have some DAB stained rat coronal sections that I would like to digitize. Basically, I want to have a picture of the entire section. It doesn¹t have to be high resolution, just enough to make out the basic structures. Can someone recommend a way to do this? I¹ve used a flat bed scanner in the past, and I know some folks use an actual film slide scanner. What I¹m interested in are tips for finding a solution, particularly what to avoid, and model numbers that people have used. I don¹t want to keep buying scanners until I found one that works. And obviously I¹d like to keep the expenses to a minimum. Finally, has anyone tried this scanner? It¹s a little pricey for me, but I do like the idea behind it, although I wonder if it¹s just one of their stock scanners that they have repainted, added an adapter and jacked up the price for. It looks identical to other scanners that they sell for $300. http://www.meyerinst.com/html/oem/pse4/ Thanks, Caroline ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Clarification-interfacing the IHC bond andCassettelabelers to Co-Path
I'd be interested in knowing how this has impacted your pathologists. Were they resistant at first? The extra time inputting requests would now fall on them. And there is still a chance of keystroke errors with pathologist entry. I'm not disagreeing with the comments you made, only wondering since you were able to save .7 FTE, where the .7 pathologist came from. Or is it the interface itself that saves the time, and not who enters it? Also, did you have the slide printers before the LEAN process was implemented? or was it done because of LEAN and is part of the FTE savings? I'm very interested in this process, but know the types of questions I'll need to have answers for. We're a Cerner facility, so some things will be different but the principal will be the same. Toni -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu]on Behalf Of Feher, Stephen Sent: Friday, October 22, 2010 1:32 PM To: Akemi Allison; Walter Benton Cc: Histonet Subject: RE: [Histonet] Clarification-interfacing the IHC bond andCassettelabelers to Co-Path We have interfaced our Bonds with Soft Path LIS system. My justification for this started with using it for LEAN processes in that the orders for IHC went directly from the Pathologist to the Bond and eliminated the need for my techs to have to input individual orders for IHC by hand. Since we have set up our slide labelers to be recognized as just another printer as far as the LIS is concerned, we do not use paper labels at all but have 2d barcodes printed directly on our slides. When an order is put in by the pathologist for IHC, my techs can see the order, cut the section and print the slide with the correct bar code. Bond recognizes the barcode and initializes the tests that have been ordered and transferred from the pathologist. This has accomplished the following: No tech time lost in printing labels for slides to go on the bond. No ambiguity or lost IHC orders due to hand writing orders by the pathologist. No chance of keystroke errors on the part of my IHC tech while putting manual orders into the Bond. In addition to eliminating hand writing and manual keystrokes, which are distinct patient safety issue, I have calculated that having the interface has saved me approximately 0.7 FTE. Instead of having to hire extra staff to cover increased workloads or wasting existing staff on extraneous tasks (hand labeling, manually entering orders, etc), I can utilize them in other areas. The patient safety aspect of eliminating extra tasks involving manual data entry is huge. A majority of the lawsuits against pathology labs involve some aspect of human error resulting from manual tasks in labeling or data entry. In addition to being able to market my lab as patient safety focused, we have eliminated a major source of potential lawsuits. It's hard to put a price tag on what that saves other than to say that the costs are sometimes much more than the dollar figures paid out. Steve -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Akemi Allison Sent: Thursday, October 21, 2010 4:01 PM To: Walter Benton Cc: Histonet Subject: Re: [Histonet] Clarification-interfacing the IHC bond and Cassettelabelers to Co-Path Hi Walter and Histo-subscribers, Ist I want to thank Walter for his quick reply. I appreciate your answer! 2nd, I appreciate any and all replies, but does anyone have an article that addresses issues that can occur such as: Efficiency Omitting Duplication of Tests ordered: Additional Slides, Special Stains, IHC, FISH, CISH, Cost effectiveness due to omission of errors Patient Safety Thanks Akemi Allison BS, HT (ASCP) HTL Director Phoenix Lab Consulting Tele: 408.335.9994 E-Mail: akemiat3...@yahoo.com On Oct 21, 2010, at 11:38 AM, Walter Benton wrote: Efficiency Patient Safety Orders for the Bond come directly from the LIS and can not be misunderstood due to poor handwriting, since they are interfaced with the LIS. Walter Benton HT(ASCP)QIHC Histology Supervisor Chesapeake Urology Associates 806 Landmark Drive, Suite 126 (All Deliveries to Suite 127) Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) wben...@cua.md From: histonet-boun...@lists.utsouthwestern.edu [histonet- boun...@lists.utsouthwestern.edu] On Behalf Of Akemi Allison [akemiat3...@yahoo.com] Sent: Thursday, October 21, 2010 2:33 PM To: Histonet Subject: [Histonet] interfacing the IHC bond and Cassette labelers to Co-Path Hi out there in Histo Land! I would like your assistance in answering a question that was proposed by a friend who is not a histonet member. I don't have the answer, but know that one of you would. Below is the question: Could you help me justify the importance of interfacing our IHC bond and
[Histonet] eosin in processor
This is the best kept secretFor those who have never tried this, you don't know what you are missing in the ease of embedding and cutting. We have been adding alcoholic eosin (about 10cc each) to the 1st 2nd 100% alcohols, leaving the last 100% pure (of course there is carry over during the week), then we dump the first and rotate the other 2 each week. The one that we now moved into position #2 will get 10cc eosin). We send our IHC FISH to a HUGE reference lab. I specifically asked about this interfering and the technical rep said that it is not a problem esp they see a lot of it and they know to avoid non specific perimeter staining (the Pathologists know how to read around this because many labs are using Eosin in the processors). J ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Cheap slide scanner for rat brains?
Caroline The older models of the Nikon scanner for kodachromes had an attachment that would scan glass slides, I'm not sure about the new ones but we had one back in the late 90's early 2000's that would hold a slide and we could scan that. You could also take some low mag images on a microscope with a camera and them merge the images in adobe elements. It will work but the method can be a bit crude and you have to watch your illumination through the entire process. I have even used a regular digital camera and taken images of the glass slide, that worked too. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Caroline Bass Sent: Friday, October 22, 2010 11:50 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cheap slide scanner for rat brains? Hello Everyone, Does anyone have a good way to get low magnification images from slides? I have some DAB stained rat coronal sections that I would like to digitize. Basically, I want to have a picture of the entire section. It doesn¹t have to be high resolution, just enough to make out the basic structures. Can someone recommend a way to do this? I¹ve used a flat bed scanner in the past, and I know some folks use an actual film slide scanner. What I¹m interested in are tips for finding a solution, particularly what to avoid, and model numbers that people have used. I don¹t want to keep buying scanners until I found one that works. And obviously I¹d like to keep the expenses to a minimum. Finally, has anyone tried this scanner? It¹s a little pricey for me, but I do like the idea behind it, although I wonder if it¹s just one of their stock scanners that they have repainted, added an adapter and jacked up the price for. It looks identical to other scanners that they sell for $300. http://www.meyerinst.com/html/oem/pse4/ Thanks, Caroline ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Clarification-interfacing the IHC bond andCassettelabelers to Co-Path
Toni, We will be going live with the Bond shortly and will have the same workflow with a different LIS. Our pathologists have been ordering their own tests for years so there is no impact, except to save time in the Immuno Lab. Victor Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 vic...@pathology.washington.edu 206-598-2792 206-598-7659 Fax = Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. On 10/22/2010 10:56 AM, Rathborne, Toni wrote: I'd be interested in knowing how this has impacted your pathologists. Were they resistant at first? The extra time inputting requests would now fall on them. And there is still a chance of keystroke errors with pathologist entry. I'm not disagreeing with the comments you made, only wondering since you were able to save .7 FTE, where the .7 pathologist came from. Or is it the interface itself that saves the time, and not who enters it? Also, did you have the slide printers before the LEAN process was implemented? or was it done because of LEAN and is part of the FTE savings? I'm very interested in this process, but know the types of questions I'll need to have answers for. We're a Cerner facility, so some things will be different but the principal will be the same. Toni -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu]on Behalf Of Feher, Stephen Sent: Friday, October 22, 2010 1:32 PM To: Akemi Allison; Walter Benton Cc: Histonet Subject: RE: [Histonet] Clarification-interfacing the IHC bond andCassettelabelers to Co-Path We have interfaced our Bonds with Soft Path LIS system. My justification for this started with using it for LEAN processes in that the orders for IHC went directly from the Pathologist to the Bond and eliminated the need for my techs to have to input individual orders for IHC by hand. Since we have set up our slide labelers to be recognized as just another printer as far as the LIS is concerned, we do not use paper labels at all but have 2d barcodes printed directly on our slides. When an order is put in by the pathologist for IHC, my techs can see the order, cut the section and print the slide with the correct bar code. Bond recognizes the barcode and initializes the tests that have been ordered and transferred from the pathologist. This has accomplished the following: No tech time lost in printing labels for slides to go on the bond. No ambiguity or lost IHC orders due to hand writing orders by the pathologist. No chance of keystroke errors on the part of my IHC tech while putting manual orders into the Bond. In addition to eliminating hand writing and manual keystrokes, which are distinct patient safety issue, I have calculated that having the interface has saved me approximately 0.7 FTE. Instead of having to hire extra staff to cover increased workloads or wasting existing staff on extraneous tasks (hand labeling, manually entering orders, etc), I can utilize them in other areas. The patient safety aspect of eliminating extra tasks involving manual data entry is huge. A majority of the lawsuits against pathology labs involve some aspect of human error resulting from manual tasks in labeling or data entry. In addition to being able to market my lab as patient safety focused, we have eliminated a major source of potential lawsuits. It's hard to put a price tag on what that saves other than to say that the costs are sometimes much more than the dollar figures paid out. Steve -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Akemi Allison Sent: Thursday, October 21, 2010 4:01 PM To: Walter Benton Cc: Histonet Subject: Re: [Histonet] Clarification-interfacing the IHC bond and Cassettelabelers to Co-Path Hi Walter and Histo-subscribers, Ist I want to thank Walter for his quick reply. I appreciate your answer! 2nd, I appreciate any and all replies, but does anyone have an article that addresses issues that can occur such as: Efficiency Omitting Duplication of Tests ordered: Additional Slides, Special Stains, IHC, FISH, CISH, Cost effectiveness due to omission of errors Patient Safety Thanks Akemi Allison BS, HT (ASCP) HTL Director Phoenix Lab Consulting Tele: 408.335.9994 E-Mail: akemiat3...@yahoo.com On Oct 21, 2010, at 11:38 AM, Walter Benton wrote: Efficiency Patient Safety Orders for the
RE: [Histonet] Cheap slide scanner for rat brains?
Do you have a dissecting scope you could use? You can grab one with a pretty low resolution camera for about $800. If you need more details let me know. Sarah Goebel, B.A., HT (ASCP) Histotechnician XBiotech USA Inc. 8201 East Riverside Dr. Bldg 4 Suite 100 Austin, Texas 78744 (512)386-2907 Original Message Subject: [Histonet] Cheap slide scanner for rat brains? From: Caroline Bass cb...@wfubmc.edu Date: Fri, October 22, 2010 10:50 am To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Hello Everyone, Does anyone have a good way to get low magnification images from slides? I have some DAB stained rat coronal sections that I would like to digitize. Basically, I want to have a picture of the entire section. It doesn¹t have to be high resolution, just enough to make out the basic structures. Can someone recommend a way to do this? I¹ve used a flat bed scanner in the past, and I know some folks use an actual film slide scanner. What I¹m interested in are tips for finding a solution, particularly what to avoid, and model numbers that people have used. I don¹t want to keep buying scanners until I found one that works. And obviously I¹d like to keep the expenses to a minimum. Finally, has anyone tried this scanner? It¹s a little pricey for me, but I do like the idea behind it, although I wonder if it¹s just one of their stock scanners that they have repainted, added an adapter and jacked up the price for. It looks identical to other scanners that they sell for $300. http://www.meyerinst.com/html/oem/pse4/ Thanks, Caroline ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] formalin containers
We haven't heard anything about this (and we were inspected at the end of July). If you have any additional information, please post it. It is a little hard to image a locking Lid on a disposable plastic container of reasonable cost that is large enough to hold a a total colon resection or large mastectomy. Bill Tench Associate Dir. Laboratory Services Chief, Cytology Services Palomar Medical Center 555 E. Valley Parkway Escondido, California 92025 bill.te...@pph.org Voice: 760- 739-3037 Fax: 760-739-2604 [None] made the following annotations - Confidential E-Mail: This e-mail is intended only for the person or entity to which it is addressed, and may contain information that is privileged, confidential, or otherwise protected from disclosure. Dissemination, distribution, or copying of this e-mail or the information herein by anyone other than the intended recipient is prohibited. If you have received this e-mail in error, please notify the sender by reply e-mail, and destroy the original message and all copies. - ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Block release
Here's what the recently revised CAP checklist says about releasing blocks; scroll down to Note 2: **REVISED** 06/17/2010 ANP.12500 Record Retention Phase II Surgical pathology records and materials are retained for an appropriate period. NOTE 1: Minimum requirements for surgical pathology, providing these are not less stringent than state and federal regulations, are: 1. Accession log records - 2 years 2. Wet tissue (stock bottle) - 2 weeks after final report 3. Paraffin blocks - 10 years (subject to Note 2, below) 4. Glass slides (including control slides) and reports - 10 years (slides must remain readable for this period) 5. Surgical pathology reports - 10 years (see Notes 3 and 4, below) 6. Fluorochrome-stained slides - at the discretion of the laboratory director 7. Fine needle aspiration slides - 10 years 8. Images of FISH studies - 10 years (see Note 5, below) There must be a documented policy for protecting and preserving the integrity and retrieval of surgical pathology materials and records.The retention period should be extended, when appropriate, to provide documentation for adequate quality control and medical care. NOTE 2: Regarding release of blocks for research purposes: Federal regulations require that a laboratory retain paraffin blocks for two years. The CLA requires, however, that they must be kept for at least 10 years. Nevertheless, blocks may be released for research purposes after the two-year regulatory requirement if all of the following criteria are met: 1. The written consent of the patient is obtained. For laboratories subject to U.S. regulations, the consent must include formal authorization in accordance with the requirements of HIPAA, if identifiable patient information is released. 2. The laboratory retains sufficient blocks to support the diagnosis for the full 10-year period. 3. Provision is made for retrieval by the laboratory of any blocks or material that remain after use in research, if the blocks or material are needed for diagnostic, legal, or other legitimate purposes. 4. The laboratory meets other relevant requirements including but not limited to the requirements of the institution, the directives of any applicable institutional review board (IRB) or similar entity; and state and local laws and regulations. NOTE 3: Pathology reports may be retained in either paper or electronic format. If retained in electronic format alone, however, the electronic reports must include a secure pathologist electronic signature. Images of paper reports--such as microfiche or PDF files--are acceptable. NOTE 4: Reports of outside consultations performed on cases from the laboratory (whether or not such consultation was requested by the laboratory) must be retained for 10 years after the date on which the original report was issued. NOTE 5: There is no retention requirement for images when the source slides remain readable for the required 10-year retention period. The 10-year retention requirement applies to images of slide preparations that are not readable for the 10-year period (e.g. FISH studies). 22 of 44 VA Med Ctr - Minneapolis Path Lab Med Service 113 Anatomic Pathology Checklist 06.17.2010 Evidence of Compliance: ✓ Written record and specimen retention policy(ies) -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bernice Frederick Sent: Monday, October 18, 2010 10:04 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Block release Everyone, I have been asked to post a query as to what your institution does in terms of releasing blocks for oncology clinical trials. Please respond as it is important to us as we receive a lot of those blocks here at Northwestern (policies, procedures, alternative submissions etc) If you are in Australia, Ireland, Canada, Puerto Rico or Peru please also answer (though I sort of know already) as we do get blocks from you also. Thanks Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Coverslip removal question
Hi Histonetters When I soak my slides in xylene to remove the coverslip, do I then need to rehydrate them by washing them in 100% etoh,95% etoh,70% etoh, and then H20. And then drying and re applying permount? Or can I go straight from xylene to drying to re-coversliping with permount? Patrick Lewis Research Associate II-Bench| Infections and Prematurity Seattle Children's Research Institute 206-884-1115 OFFICE 000-000- PAGER 000-000- CELL 206-884-7311 FAX patrick.le...@seattlechildrens.org OFFICE 1900 9th Avenue Seattle, WA 98101 MAIL M/S C9S-8, Seattle, WA 98101 WWW seattlechildrens.org http://seattlechildrens.org/ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Coverslip removal question
No, once you have removed the coverslip, just wash the section thoroughly in xylene to remove any thick remnant of the old permount, and reapply permount and cover. René J. --- On Fri, 10/22/10, Lewis, Patrick patrick.le...@seattlechildrens.org wrote: From: Lewis, Patrick patrick.le...@seattlechildrens.org Subject: [Histonet] Coverslip removal question To: Histonet@lists.utsouthwestern.edu Date: Friday, October 22, 2010, 3:55 PM Hi Histonetters When I soak my slides in xylene to remove the coverslip, do I then need to rehydrate them by washing them in 100% etoh,95% etoh,70% etoh, and then H20. And then drying and re applying permount? Or can I go straight from xylene to drying to re-coversliping with permount? Patrick Lewis Research Associate II-Bench| Infections and Prematurity Seattle Children's Research Institute 206-884-1115 OFFICE 000-000- PAGER 000-000- CELL 206-884-7311 FAX patrick.le...@seattlechildrens.org OFFICE 1900 9th Avenue Seattle, WA 98101 MAIL M/S C9S-8, Seattle, WA 98101 WWW seattlechildrens.org http://seattlechildrens.org/ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Recommendations for decal solution
Hi guys, Can anyone recommend a good decal solution. I have some bone marrow and trachea tissues for paraffin sectioning and I want to decal them. Thanks Patrick Patrick Lewis Research Associate II-Bench| Infections and Prematurity Seattle Children's Research Institute 206-884-1115 OFFICE 000-000- PAGER 000-000- CELL 206-884-7311 FAX patrick.le...@seattlechildrens.org OFFICE 1900 9th Avenue Seattle, WA 98101 MAIL M/S C9S-8, Seattle, WA 98101 WWW seattlechildrens.org http://seattlechildrens.org/ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Recommendations for decal solution
I like 5 to 10% formic acid Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lewis, Patrick Sent: Friday, October 22, 2010 2:06 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Recommendations for decal solution Hi guys, Can anyone recommend a good decal solution. I have some bone marrow and trachea tissues for paraffin sectioning and I want to decal them. Thanks Patrick Patrick Lewis Research Associate II-Bench| Infections and Prematurity Seattle Children's Research Institute 206-884-1115 OFFICE 000-000- PAGER 000-000- CELL 206-884-7311 FAX patrick.le...@seattlechildrens.org OFFICE 1900 9th Avenue Seattle, WA 98101 MAIL M/S C9S-8, Seattle, WA 98101 WWW seattlechildrens.org http://seattlechildrens.org/ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Lab Manager and other Histology Jobs
I am one of the founders of a healthcare recruiting firm. I help Lab Professionals find permanent employment and I wanted to see if you or anyone you know is interested in exploring other career opportunities? We are completely free of charge to candidates and and we work on laboratory openings across the country. Our clients typically assist with relocation expenses. I am currently working on some great positions that you may be interested in including a Laboratory Manager opening at a Dermpath Lab. My client is a Pre-IPO Pathology company who is quickly establishing itself as an industry leader. They are looking for someone to step into a Lab Manager position that they have open in their Dermpath Lab in Long Island, NY. This state of the art lab s looking for someone with prior management experience to run and manage a lab consisting of 10 FTE's. This Manager will be responsible for overseeing the day to day operations of the lab. My clients offers one of the best compensation benefits package around including relocation assistance when necessary. For consideration you must have a Bachelor's Degree, be HT or HTL (ASCP) certified and licensed in the State of NY. A minimum of 2 years of prior supervisory or management experience is preferred. If you are interested in learning more about this position, please call or email me at k...@ka-recruiting.com Below is a list of some of the other opportunities we are currently working on. If you do not see an opening in a location in which you live or would like to live, please send me an email me a copy of your resume and let me know where you would be interested in a job. I will then tailor a search for you that is completely confidential and free to candidates. Current Histology Openings: NY - Upstate - Histotechnologist MI - Histology Manager NY - New York City - Histotechnologist - 3rd shift OK - Histology Supervisor GA - Histology Supervisor NV - Las Vegas - Histotechnologist - 3rd shift TN - Histotechnologist TX - Histology Supervisor If you're interested in any of these opportunities or are currently looking for employment then please send me an updated resume and let me know when you're available to talk. If this is not the right time for you to make a career change then please let me know if you can refer someone who is. We offer a generous referral bonus for anyone you refer to us that we place into any position across the country. To learn more about job openings in other parts of the country please contact me or visit our website at www.ka-recruiting.com. Sincerely, KC Carpenter K.A. Recruiting, Inc. 10 Post Office Square, 8th Floor South Boston, MA 02109 P: (617) 692-2949 F: (617) 507-8009 k...@ka-recruiting.com www.ka-recruiting.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Tissue Processor Advice
We are in need of some advice regarding rapid tissue processors. Models we are considering: Sakura Xpress Leica Peloris Thermo STP 420 It seems none of these models are perfect in every respect. I'm interested in anyone's opinions of these processors and your experience with them. All input is appreciated! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] eosin in processor
We don't, Leica recommends that you don't. We have our grossing group squirt a 0.1% Basic Fuchsin on the small translucent or white specimens. It leaves it light pink like the eosin. On Fri, Oct 22, 2010 at 11:09 AM, Rathborne, Toni trathbo...@somerset-healthcare.com wrote: Does anyone use eosin in their Peloris? -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu]on Behalf Of Shea's Sent: Friday, October 22, 2010 2:07 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] eosin in processor This is the best kept secretFor those who have never tried this, you don't know what you are missing in the ease of embedding and cutting. We have been adding alcoholic eosin (about 10cc each) to the 1st 2nd 100% alcohols, leaving the last 100% pure (of course there is carry over during the week), then we dump the first and rotate the other 2 each week. The one that we now moved into position #2 will get 10cc eosin). We send our IHC FISH to a HUGE reference lab. I specifically asked about this interfering and the technical rep said that it is not a problem esp they see a lot of it and they know to avoid non specific perimeter staining (the Pathologists know how to read around this because many labs are using Eosin in the processors). J ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Patrick Laurie HT(ASCP)QIHC CellNetix Pathology Laboratories 1124 Columbia Street, Suite 200 Seattle, WA 98104 plau...@cellnetix.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] decal solution help
Dear Histonetters Yes I should have said, I'll be doing IHC for viral proteins with both polyclonal (rabbit) and monoclonal (Rat/Mouse) primary antibodies and HRP-labeled secondary antibodies with DAKO AEC Substrate. Patrick Lewis Research Associate II-Bench| Infections and Prematurity Seattle Children's Research Institute 206-884-1115 OFFICE 000-000- PAGER 000-000- CELL 206-884-7311 FAX patrick.le...@seattlechildrens.org OFFICE 1900 9th Avenue Seattle, WA 98101 MAIL M/S C9S-8, Seattle, WA 98101 WWW seattlechildrens.org http://seattlechildrens.org/ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Recommendations for decal solution
Best decal in the world...Formical-4. It has formalin in it so it fixes and decals at the same time. You can get plain Jane decal too. The company is called decal. Here is the website. I think you can also get it from Fisher? http://www.decal-bone.com/ Sarah Goebel, B.A., HT (ASCP) Histotechnician XBiotech USA Inc. 8201 East Riverside Dr. Bldg 4 Suite 100 Austin, Texas 78744 (512)386-2907 Original Message Subject: [Histonet] Recommendations for decal solution From: Lewis, Patrick patrick.le...@seattlechildrens.org Date: Fri, October 22, 2010 1:06 pm To: Histonet@lists.utsouthwestern.edu Hi guys, Can anyone recommend a good decal solution. I have some bone marrow and trachea tissues for paraffin sectioning and I want to decal them. Thanks Patrick Patrick Lewis Research Associate II-Bench| Infections and Prematurity Seattle Children's Research Institute 206-884-1115 OFFICE 000-000- PAGER 000-000- CELL 206-884-7311 FAX patrick.le...@seattlechildrens.org OFFICE 1900 9th Avenue Seattle, WA 98101 MAIL M/S C9S-8, Seattle, WA 98101 WWW seattlechildrens.org http://seattlechildrens.org/ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Recommendations for decal solution
For the specimens you mention use EDTA pH7 René J. --- On Fri, 10/22/10, Lewis, Patrick patrick.le...@seattlechildrens.org wrote: From: Lewis, Patrick patrick.le...@seattlechildrens.org Subject: [Histonet] Recommendations for decal solution To: Histonet@lists.utsouthwestern.edu Date: Friday, October 22, 2010, 4:06 PM Hi guys, Can anyone recommend a good decal solution. I have some bone marrow and trachea tissues for paraffin sectioning and I want to decal them. Thanks Patrick Patrick Lewis Research Associate II-Bench| Infections and Prematurity Seattle Children's Research Institute 206-884-1115 OFFICE 000-000- PAGER 000-000- CELL 206-884-7311 FAX patrick.le...@seattlechildrens.org OFFICE 1900 9th Avenue Seattle, WA 98101 MAIL M/S C9S-8, Seattle, WA 98101 WWW seattlechildrens.org http://seattlechildrens.org/ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Tissue Processor Advice
We are using the Peloris with a 2 hr, 4 hr and 8 hr protocol. We run 2 hour protocols throughout the day with an average of 4-5 runs per day depending on specimen volume. We really like this processor. We have had them for 10 months now, are using factory protocols and have not had any specimens that have been either under or over processed. The techs and the pathologists are very pleased with it. Steve -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of caymanfl...@gmail.com Sent: Friday, October 22, 2010 4:17 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Tissue Processor Advice We are in need of some advice regarding rapid tissue processors. Models we are considering: Sakura Xpress Leica Peloris Thermo STP 420 It seems none of these models are perfect in every respect. I'm interested in anyone's opinions of these processors and your experience with them. All input is appreciated! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Tissue Processor Advice
How are you meeting the hours of fixation requirement for Breast? With 2 and 4 and 8 hours,, But recently there are articles calling for Her 2 to be done on GI cases. Just want to know you insight on this? -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Feher, Stephen Sent: Friday, October 22, 2010 2:12 PM To: caymanfl...@gmail.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Tissue Processor Advice We are using the Peloris with a 2 hr, 4 hr and 8 hr protocol. We run 2 hour protocols throughout the day with an average of 4-5 runs per day depending on specimen volume. We really like this processor. We have had them for 10 months now, are using factory protocols and have not had any specimens that have been either under or over processed. The techs and the pathologists are very pleased with it. Steve -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of caymanfl...@gmail.com Sent: Friday, October 22, 2010 4:17 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Tissue Processor Advice We are in need of some advice regarding rapid tissue processors. Models we are considering: Sakura Xpress Leica Peloris Thermo STP 420 It seems none of these models are perfect in every respect. I'm interested in anyone's opinions of these processors and your experience with them. All input is appreciated! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. __ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Aqua-Mount?
Is Aqua-Mount aqueous mounting medium still available? If so, who sells it? If not, recommendations for a similar product? Thanks Paul ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Aqua-Mount?
We use an aqueous mounting media from Diagnostic Biosystems. Available in two sizes. Same as the aqua mount Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Monfils, Paul [pmonf...@lifespan.org] Sent: Friday, October 22, 2010 6:27 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Aqua-Mount? Is Aqua-Mount aqueous mounting medium still available? If so, who sells it? If not, recommendations for a similar product? Thanks Paul ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Tissue Processor Advice
There are so many good to great processors on the market, but all have their plus and minus issues. You really have to decide what your two or three most important issues will be and then rank them. With the trend in becoming more efficient/cost effective, reducing TAT and LEAN process improvement, I suggest you look to improve your process to match these trends and you will be lead to rapid tissue processing in a LEAN way. Couple the previously mentioned trends/issues with versatility of processing with your routine formalin fixed samples with molecular fixed samples on the same instrument, I suggest the Sakura Xpress (X50 or X120) rapid processors. These instruments provide continuous loading, small batch and require a small volume of reagent for processing and then discard. The instruments do have a required reagent kit and there is a variable pre-processing protocol, depending on the tissue type and fat content. Using the reagent kit does allow for cost savings over conventional processing and I find the pre-processing allows for better precision processing techniques and protocols, we have never over processed tissues. Another great advantage is the increased velocity of the workflow as the instruments are continuous load (no cleaning cycle between batches) and small batch (1 to 40 cassettes). Loading 1 or 2 cassettes when a STAT or RUSH cases arrives and completes fixation does not interrupt the process or require special handling. An important factor to consider is that continuous load processing does assist in workload leveling, which can assist in reducing employee stress, increase productivity and error reduction. All these things lead directly to reduced TAT. Add the often overlooked advantage of removing Xylene from your tissue processing, and again, I suggest you consider the Xpress. I was an early adopter (5+ yrs use) and continue to use the X120 (2 units). I have not experienced any instrument performance or maintenance issues. I have had three software upgrades and the instruments had to go down for several hours to install the upgrade. The X120 and now the X50 have two programming options 1+ hour or 2+ hour processing. The most LEAN factor is that after the first basket of up to 40 cassettes, the next one comes off 20 or 40 minutes later and you can continuously load. There is no other instrument that can allow you to process in as small as batch or provide the continuous delivery of cassettes. You can do rapid processing with all of the instruments you are considering, but conventional, one reaction chamber instruments will limit the number of times the instrument can be run each day and that increases the batch size. Rapid processing does demand change in the way your lab does it's work. The first is standardization of gross dissection to 3 mm thickness. It does not matter the instrument, if you want to truly move into rapid processing, then you have to standardize your process in the gross room. The process of retraining and standardizing your gross room is well worth the effort. Another issue that you have to fully consider is how will specific fixation times affect your workflow. We now have specific guidelines for ER/PgR; Her2 and if you follow the NCI protocols for cancer tumors, you have to record your actual fixation times. Trying to manage all the different fixation times becomes difficult and will slow down your process. If you use your tissue processor to complete fixation, the processor will force you into larger batches. When you have to wait for tissues to complete fixation before starting your processing program, you also limit the number of times the processor can be run in a day. I find that separating fixation from processing is the best approach. You process specimens only when the optimal fixation time has been completed. Stop and consider all the different tissue types, size, fat content and required protocols, and you will see the value of a rapid processor that has small batch and continuous load capabilities. Meet all your fixation requirements and needs and only use the tissue processor for processing, not fixation. This is very LEAN concept and a concept that I believe you will need to embrace. Whenever you have the opportunity to change your process, I always suggest you look to improve the process, use the latest process improvement techniques and select an instrument that will assist in the change and prepare you for future change. My philosophy is we cannot continue to do tomorrow what we do today and expect a different outcome or result. Just my thoughts and experience, I hope this will help you. Do not hesitate to contact me if you have any questions. William DeSalvo, B.S., HTL(ASCP) Date: Fri, 22 Oct 2010 16:16:50 -0400 From: caymanfl...@gmail.com To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Tissue Processor Advice We are in need of some advice regarding rapid tissue
