RE: [Histonet] PAS with Diastase Digestion
Not spit ,saliva and we leave it on for 15/20 minutes and then rinse well. I have had techs who did not like doing it so we bought the enzyme and after all the hassle she found it did not work as well...now we all think about chocolate and salivate. :) -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Erin Sarricks Sent: Wednesday, February 09, 2011 7:38 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] PAS with Diastase Digestion Hi Histonet- I recently ran a PAS/D stain and had some issues with it. Both with and without slides came out looking the same so I'm guessing my digestion step didn't work! I used a Malt Diastase solution (0.5g to 500mL water) for my digestion. This is the procedure I used: 1.Deparaffinize and hydrate to water. 2.Place the sections labeled “with” in diastase solution preheated to 37˚C for 1 hour. Hold the sections labeled “without” in distilled water. 3.Wash in running water for 5 minutes 4.Place all section (with and without) in 0.5% periodic acid solution for 5 minutes 5.Wash in 3 changes of distilled water 6.Place in Schiff reagent for 15 minutes 7.Wash in lukewarm tap water for 5 minutes (immediately sections turn dark pink color). 8.Counterstain in Mayer’s Hematoxylin for 3 minutes. 9.Wash in tap water for 10 minutes 10. Dehydrate starting with 95% ETOH, clear, and coverslip. I am wondering if my solution possibly got too warm in the oven and hindered the enzyme activity, or is it possible I left it in too long? Any tips would be much appreciated! Oh, and I have about 300 slides to stain, so spitting on them is my last last last resort! Haha! Thanks in advance for all your help! Erin Sarricks, HT (ASCP) Histology Laboratory Technician USAMRICD Comparative Pathology Branch Office: Bldg E-3081 Room 178 E-mail: erin.p.sarri...@us.army.mil ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] FFPE tissue - sucrose fixing necessary?
This sounds like something you would do before freezing not processing to paraffin. Miss Margaret Blount Histology Manager Metabolic Research Laboratories Level 4 Institute of Metabolic Science Box 289, Addenbrooke's Hospital Hills Road, Cambridge, CB2 0QQ Tel 01223 769061/336079 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Abbey Mortimer Sent: 10 February 2011 02:22 To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] FFPE tissue - sucrose fixing necessary? Hello helfpul Histonetters! I am curious about your formalin-fixed paraffin embedded (FFPE) tissue protocols. If the tissue has been fixed with saline and paraformaldehyde during perfusion, then further fixed in paraformaldehyde for 24 hours, do you still utilise a sucrose wash before running it through the automatic tissue processor? Or do you not need this step? I have heard mixed responses about this and wonder if any of you have had experience with this! Thanks so much, Abbey ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] IHC validation
Added to this.make sure you keep your work up sheets. Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.) AP Supervisor Shore Memorial Hospital 609-653-3590 Speak only well of people and you need never whisper Rene J Buesa rjbu...@yahoo.co m To Sent by: Histonet histonet-bounces@ histonet@lists.utsouthwestern.edu lists.utsouthwest , Joe Nocito jnoc...@satx.rr.com ern.educc Subject 02/09/2011 03:26 Re: [Histonet] IHC validation PM Joe: This is what I would do; 1- run 1 control slide per antibody you have in your arsenal 2- compare the result with a control slide already in your files. 3- show both slides to the chief pathologist (after all is his/her opinion the one is going to be asked by CAP) 4- those antibodies whose positive controls reacted substantially different to those in your files are the ones you have to work with with respect to concentration or detection method. 5- never overdue it, and avoid excessive costs that usually are never appreciated. Rely always in your pathologist's opinion René J. --- On Tue, 2/8/11, Joe Nocito jnoc...@satx.rr.com wrote: From: Joe Nocito jnoc...@satx.rr.com Subject: [Histonet] IHC validation To: Histonet histonet@lists.utsouthwestern.edu Date: Tuesday, February 8, 2011, 5:43 PM Greetings Histoland, I need some help. We are about to switch IHC machines from the Richard-Allen Axiom to the Ventana Benchmark Ultra. How many slides, per antibody, do you run for the validation study? We have over 100 primary antibodies. Normally, when we work up a new antibody, we start with a titer. Once that is established, we run 10 cases to check for specificity. Hopefully we can obtain cases that are really positive, some weakly positive and some flat out negative. Once that is completed, we run 10 different tissue types to check for any unexpected cross-reactivity. The ultra holds 30 slides and we are receiving two machines. If we run 10 slides/antibody, that's going to take a while, not to mention the number of detection kits that will be used. Do you think 5 slides/antibody is sufficient? I emailed CAP last week for their take and they never returned my email (I told my medical director to hold their check for the year and see how fast they respond to that). Ah oh, don't go down that road Joe, it's unhealthy. What are your thoughts? Thanks Joe (JTT) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] CPT code 88363
Is this a code that can be used even if you have your molecular done somewhere else? Mike -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Podawiltz, Thomas Sent: Wednesday, February 09, 2011 2:43 PM To: Richard Cartun; Histonet Subject: RE: [Histonet] CPT code 88363 It is my understanding that it used for examaination and selection of retrieved archival (ie,previously diagnosed) tissue for molecular analysis (eg, KRAS mutational analysis). page 440 of the 2011 AMA CPT , professional edition. Tom Podawiltz, HT (ASCP) Histology Section Head/Laboratory Safety Officer LRGHealthcare 603-524-3211 ext: 3220 From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Richard Cartun [rcar...@harthosp.org] Sent: Wednesday, February 09, 2011 1:12 PM To: Histonet Subject: [Histonet] CPT code 88363 Is anyone using the above CPT code for reviewing old pathology slides prior to ordering IHC testing or can it only be used for slide review prior to ordering molecular testing? Thanks. Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] QIHC
Read and digest the Dako book and you will sail through the exam. For a specialist type qualification it really is pitiful. Ronnie -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Michael Bradley Sent: Wednesday, February 09, 2011 4:30 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] QIHC Hi all I am preparing to take the QIHC exam. Does anyone have any suggestions on what I should study? What areas does the exam concentrate on and what research material I can use. Thanks ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet - Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] QIHC
Hi Michael, I used NSH 5th series assessment book, double check on the NSH website. It's the book with questions and answers for IHC, Enzyme histochemistry. I also used IHC tutorial by Thermo Shandon online, that was recommended on the histonet network. I'm not sure of the website. Maybe someone has that information. Both of these tools helped me when I passed in April 2009. Steph -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Michael Bradley Sent: Wednesday, February 09, 2011 4:30 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] QIHC Hi all I am preparing to take the QIHC exam. Does anyone have any suggestions on what I should study? What areas does the exam concentrate on and what research material I can use. Thanks ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Cassette labeling
We use B. Nancy Schmitt MLT, HT(ASCP) United Clinical Laboratories Dubuque, IA Message: 9 Date: Tue, 8 Feb 2011 16:54:54 -0500 From: Lee Mayhew lmayh...@cogeco.ca Subject: [Histonet] Cassette labeling To: Histonet Histonet@lists.utsouthwestern.edu Message-ID: 82d56d94-90d4-4137-88d1-4c0ede21f...@cogeco.ca Content-Type: text/plain; charset=us-ascii Hi Histonetters, At my hospital, we are having a discussion about how to label cassettes. I have worked at 2 hospitals, and they each do it a different way. Our cassette labeller will print either way. Could you please indicate which way you do it at your site, A or B. A..When the cassette is open and sitting on the bench facing you with the lid on the far side and the surface for writing on is closest to you, the surgical number is upside down. B.When the cassette is open and sitting on the bench facing you with the lid on the far side and the surface for writing on is closest to you, the surgical number is right side up. Thanks in advance. Lee Mayhew MLT St. Josephs Hospital Hamilton, ON Canada -- NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Supervisory/Lead Job in Fort Myers, FL
*Position Title: *Histotech III (Lead)/Supervisory *Reports To: *Laboratory Director *Shift: *Monday-Friday, 8am-5pm * * *Location:* * * Pathology Laboratory for well established busy office in Fort Myers, FL founded in 2000. We are looking at local candidates for now, but may be willing to help with relocation of in state applicants. * * *Requirements:* * * - ASCP certification required** - Experience leading a team of 3+ laboratory staff memebers** - Experience and knowledge of OSHA, CLIA, AHCA regulations** - The ability to implement and maintain QA/QC policies and procedures** - Oversee safety and training** - Understanding of the different lab licensing and ranges of testing** * * *Summary:* * * - Responsible for 2 other employees within the laboratory** - Work with the Director of Compliance on compliancy of the lab** - Handles all laboratory compliance** - Occasionally help with day to day routine histology ** ** * * *Benefits:* Health Insurance, Dental Insurance, Vision Insurance. Group Life, Short Term Disability and Long Term Disability paid by employer. Voluntary Life for self, spouse, and child. Discount on employer products and services. PTO for vacation, personal time, sick etc. Paid Holidays (7): New Years Day, Memorial Day, Fourth of July, Labor Day, Thanksgiving Day, Day after Thanksgiving Day, and Christmas Day. Bereavement Leave.* *Bi-annual bonuses, performance reviews, uniform and name badge. Direct Depost 401K with employer contribution. *To apply:* * * Please send resume and salary expectations to aly...@alliedsearchpartners.com . At that time we will contact you to conduct a phone screen. Thank you! -- * * **If you wish to no longer receive emails from Allied Search Partners please reply with “Remove.” * Alyssa Peterson, Director of Candidate Recruitment LinkedIN:http://www.linkedin.com/in/alyssapetersonasp Allied Search Partners T: 888.388.7571 F: 888.388.7572 www.alliedsearchpartners.com This email including its attachments is intended only for the confidential use of the individual to whom it is addressed. If you are not the intended recipient, any use, dissemination, distribution or copying of this message or its attachments is prohibited. If you have received this message in error, please notify us immediately, and delete this message and its attachments permanently from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] QIHC
Its true, that test is too easy if you ask me. Dako handbook is all you'll need. Mark On Thu, Feb 10, 2011 at 5:46 AM, Houston, Ronald ronald.hous...@nationwidechildrens.org wrote: Read and digest the Dako book and you will sail through the exam. For a specialist type qualification it really is pitiful. Ronnie -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto: histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Michael Bradley Sent: Wednesday, February 09, 2011 4:30 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] QIHC Hi all I am preparing to take the QIHC exam. Does anyone have any suggestions on what I should study? What areas does the exam concentrate on and what research material I can use. Thanks ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet - Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Histotech 2 position available in Portland, OR with Providence Health and Services
Dear Histology Professionals, (Please, no Recruiters) If you are interested in exploring a move to the Pacific NW, please email me regarding the following opportunity: Providence is calling a full-time Histotechnician II for an evening shift position at Providence Portland Medical Center in Portland, OR. Providence Health and Services in Oregon is seeking a passionate Histology professional to join our team in Pathology to accurately prepare material for study and diagnostic interpretation by pathologists. Histology personnel will perform pre-analytical, analytical and post-analytical duties including tissue processing, routine stains, special stains, immunohistochemical stains, instrument maintenance and problem resolution. Histology personnel work with acute-care and outpatient caregivers, vendors, consultants and other Laboratory Services staff in performing their duties. Our Pathology department serves 7 hospitals and multiple outpatient clinics in a challenging, state of the art department. We offer competitive compensation and a distinguished benefits plan. The requirements for this position include: * For HT, HTL with Bachelors degree: 2-5 years of industry related experience (**). For HT from accredited training program or OJT: 3-6 years of industry related (**) experience. (**) Industry related at Level 2 requires that the experience be from an equivalent high volume or acute care laboratory performing high complexity testing. Answer the call to fulfill the Mission. Providence Health Services is a not-for-profit organization extending across a five-state area - from Alaska through Washington, Montana, Oregon, and into Southern California. Providence employs more than 45,000 employees, and operates 27 acute care hospitals, and more than 35 non-acute health care facilities, as well as physician clinics, health plans, and numerous other health and education services. When you're ready to pursue an outstanding career opportunity that offers more than a competitive salary and an excellent variety of benefits, consider Providence Health Services-Oregon. Please send me your resume and/or any questions you have about this opportunity. Kind Regards, Annette M Fletcher Senior Recruiter annette.fletc...@providence.org Providence Health Services, Talent Acquisition Respect | Compassion | Justice | Excellence | Stewardship This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] p16 antibody
Hi everyone was wondering who carries the IVD p16 antibody? Is it only MTM? Thanks in advance. Michele Carr HTL ASCP Medical Laboratory Services ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] p16 antibody
Yes. They hold the patent. On Feb 10, 2011 1:30 PM, Michele Carr michelecar...@yahoo.com wrote: Hi everyone was wondering who carries the IVD p16 antibody? Is it only MTM? Thanks in advance. Michele Carr HTL ASCP Medical Laboratory Services ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] p16 antibody
MTM has the patent rights so they are the only one. Walter Benton HT(ASCP)QIHC Histology Supervisor Chesapeake Urology Associates 806 Landmark Drive, Suite 126 (All Deliveries to Suite 127) Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) wben...@cua.md From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Michele Carr [michelecar...@yahoo.com] Sent: Thursday, February 10, 2011 1:29 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] p16 antibody Hi everyone was wondering who carries the IVD p16 antibody? Is it only MTM? Thanks in advance. Michele Carr HTL ASCP Medical Laboratory Services ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] p16 antibody
They hold the patent and no one else produces it. We order the kit and use the antibody on the Ventana XT stainers, placing the antibody in a prep kit. Works great. Mark Turner, PhD, HT(ASCP)QIHC Mayo Clinic in Arizona Michele Carr michelecar...@yahoo.com wrote: Hi everyone was wondering who carries the IVD p16 antibody? Is it only MTM? Thanks in advance. Michele Carr HTL ASCP Medical Laboratory Services ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] tungsten carbide knives
Hi If anyone has any used tungsten carbide knives that they no longer need please let me know. These should be in good shape and suitable for sharpening. I'm happy to pay a resonable price for them. Thanks Adam --- On Thu, 2/10/11, histonet-requ...@lists.utsouthwestern.edu histonet-requ...@lists.utsouthwestern.edu wrote: From: histonet-requ...@lists.utsouthwestern.edu histonet-requ...@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 87, Issue 20 To: histonet@lists.utsouthwestern.edu Date: Thursday, February 10, 2011, 1:51 PM Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than Re: Contents of Histonet digest... Today's Topics: 1. RE: QIHC (Houston, Ronald) 2. RE: CPT code 88363 (Mike Pence) 3. RE: QIHC (Stephanie Rivera) 4. Cassette labeling (Nancy Schmitt) 5. HT Position in Oklahoma City (Gaiser, Marcia) 6. Supervisory/Lead Job in Fort Myers, FL (Alyssa Peterson) 7. RE: CPT code 88363 (Richard Cartun) 8. Re: QIHC (Mark Tarango) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Position Open in NJ/NY area
Allied Search Partners is currently accepting resumes for a histotechnician/histotechnologist. Ideally we are looking for a candidate with at least 3 years experience. Location: Clifton, NJ area *Essential Functions and Duties* Must be able to work independently under minimal supervision, maintain laboratory supplies, equipment, and QC/QA records Must be knowledgeable with basic computer skills and pathology software, and participate in improvement of laboratory procedures. The person should be reliable with great inter-personal communication skills, and willing to coordinate with other departmental staff. Need someone with great cutting, grossing skills. *Shift:* Day Shift Hours *Requirements* Bachelor’s degree preferred but not required HT (ASCP) preferred The Bachelor degree may be waived if the candidate has extensive experience (5 years) At least 3 years of histotechnology experience including routine histology, immunohistochemistry staining (automated), special stain (manual, for bone marrow and others). To apply for this position please submit resume to aly...@alliedsearchpartners.com for initial prescreening. No resume will be submitted to client until we speak to you for a phone interview. All resumes kept confidential. -- * * **If you wish to no longer receive emails from Allied Search Partners please reply with “Remove.” * Alyssa Peterson, Director of Candidate Recruitment LinkedIN:http://www.linkedin.com/in/alyssapetersonasp Allied Search Partners T: 888.388.7571 F: 888.388.7572 www.alliedsearchpartners.com This email including its attachments is intended only for the confidential use of the individual to whom it is addressed. If you are not the intended recipient, any use, dissemination, distribution or copying of this message or its attachments is prohibited. If you have received this message in error, please notify us immediately, and delete this message and its attachments permanently from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Need help with interpretation: Gomori Trichrome / MELAS-Syndrom
Hi all, I'm reading about OXPHOS diseases (defects in mitochondrial protein subunits of respiratory chain). RFF in MERRF stands for red ragged fibers, see: http://en.wikipedia.org/wiki/File:Ragged_red_fibers_in_MELAS.jpg I don't get what is stained there exactly. The picture shows a muscle biopsy stained with Gomori's trichrome stain.. What is stained in these red fibers? I'm sure one of you has some experience with that. Thanks, V. Neubert ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] bar-coded chemical inventory
Anyone out there using barcode systems to inventory chemicals and organize MSDS, expiration dates, lot numbers, NFPA, etc? If so, what system and how well does it work? In particular I'm curious about the CISpro system. Many thanks! Brandon This electronic message transmission contains information which may be confidential or privileged. The information is intended to be for the use of the individual or entity named above. If you are not the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this information is prohibited. If you have received this electronic transmission in error, please leave a message via telephone at (206) 624-1159, notify me by electronic reply, and delete this message. Opinions and ideas in this message that do not relate to official business are understood as neither given nor endorsed by the Seattle Cancer Care Alliance. To view our complete Notice of Privacy Practices, visit our web site at www.seattlecca.org. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Help with OCT problem
Has anyone ever experienced OCT blocks that cut like they are soft. I have turned the cyrostat down 10 degrees lower than usual and they are still soft. Really weird part is that I have 20 blocks in this group and some are soft and others are just fine. Tried changing angle, new blade nothing helps. When I finally manage to get a decent section and pick it up on the slide, the OCT around the tissue want even pick up smooth it wrinkles really bad. I tried just a block of OCT that I froze on a chuck and it cut great. I am clueless as to what is going on. Samples were frozen by someone else and brought to me to be cut. Donna Reynolds Core IHC Lab M.D. Anderson Cancer Center, Houston TX Research lab 713-192-8106. Donna Reynolds Core IHC Lab Dept. Cancer Biology, SRB 1.660 713-792-8106 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] bar-coded chemical inventory
I would be interested in that info too. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Seaton, Brandon W Sent: Thursday, February 10, 2011 12:37 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] bar-coded chemical inventory Anyone out there using barcode systems to inventory chemicals and organize MSDS, expiration dates, lot numbers, NFPA, etc? If so, what system and how well does it work? In particular I'm curious about the CISpro system. Many thanks! Brandon This electronic message transmission contains information which may be confidential or privileged. The information is intended to be for the use of the individual or entity named above. If you are not the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this information is prohibited. If you have received this electronic transmission in error, please leave a message via telephone at (206) 624-1159, notify me by electronic reply, and delete this message. Opinions and ideas in this message that do not relate to official business are understood as neither given nor endorsed by the Seattle Cancer Care Alliance. To view our complete Notice of Privacy Practices, visit our web site at www.seattlecca.org. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Help with OCT problem
Donna I have seen soft or rather sticky OCT samples in the past. If you freeze in isopentane and don't let the isopentane evaporate off the samples prior to wrapping the sample in foil, that excess isopentane changes the OCT, it makes it sticky. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Reynolds,Donna M Sent: Thursday, February 10, 2011 12:39 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Help with OCT problem Has anyone ever experienced OCT blocks that cut like they are soft. I have turned the cyrostat down 10 degrees lower than usual and they are still soft. Really weird part is that I have 20 blocks in this group and some are soft and others are just fine. Tried changing angle, new blade nothing helps. When I finally manage to get a decent section and pick it up on the slide, the OCT around the tissue want even pick up smooth it wrinkles really bad. I tried just a block of OCT that I froze on a chuck and it cut great. I am clueless as to what is going on. Samples were frozen by someone else and brought to me to be cut. Donna Reynolds Core IHC Lab M.D. Anderson Cancer Center, Houston TX Research lab 713-192-8106. Donna Reynolds Core IHC Lab Dept. Cancer Biology, SRB 1.660 713-792-8106 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] CPT code 88363
No; you can not bill the patient for this. You should charge the attorney for the work you do. Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax DANIEL HEWITT dhew...@hvhs.org 2/10/2011 3:25 PM How about pulling blocks and cutting blank slides for an attorney who does not represent our hospital? We don't have to do this very much but it does come up. Thanks Daniel Hewitt Histology Supervisor, HVS 412-749-7371 This email, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, or an agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and delete and destroy all copies of the original message, including attachments. Please note that any views or opinions presented in this e-mail are solely those of the author and do not necessarily represent those of Heritage Valley Health System. The integrity and security of this message cannot be guaranteed on the internet. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Thursday, February 10, 2011 11:23 AM To: Mike Pence; Histonet; Thomas Podawiltz Subject: RE: [Histonet] CPT code 88363 I think so. Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax Mike Pence mpe...@grhs.net 2/10/2011 9:19 AM Is this a code that can be used even if you have your molecular done somewhere else? Mike -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Podawiltz, Thomas Sent: Wednesday, February 09, 2011 2:43 PM To: Richard Cartun; Histonet Subject: RE: [Histonet] CPT code 88363 It is my understanding that it used for examaination and selection of retrieved archival (ie,previously diagnosed) tissue for molecular analysis (eg, KRAS mutational analysis). page 440 of the 2011 AMA CPT , professional edition. Tom Podawiltz, HT (ASCP) Histology Section Head/Laboratory Safety Officer LRGHealthcare 603-524-3211 ext: 3220 From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Richard Cartun [rcar...@harthosp.org] Sent: Wednesday, February 09, 2011 1:12 PM To: Histonet Subject: [Histonet] CPT code 88363 Is anyone using the above CPT code for reviewing old pathology slides prior to ordering IHC testing or can it only be used for slide review prior to ordering molecular testing? Thanks. Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Her2 requests on decal specimens
I am hoping to get some guidance on this. Occasionally we get requests to perform HER2 on specimens that have been decaled. The revised CAP question, ANP.22998 specifically states in the notes that decalcification with strong acids should not be used. How are other institutions handling this? Martha Ward, MT (ASCP) QIHC Assistant Manager Molecular Diagnostics Lab Dept. of Pathology Wake Forest University Baptist Medical Center Winston-Salem, NC 27157 336-716-2104 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Help with OCT problem
Donna, I would also check to make sure that the tissue wasn't held in an something with a lot of alcohol, like RNA later. I found out first hand that it doesn't work well. Good luck On Thu, Feb 10, 2011 at 11:38 AM, Reynolds,Donna M dreyn...@mdanderson.org wrote: Has anyone ever experienced OCT blocks that cut like they are soft. I have turned the cyrostat down 10 degrees lower than usual and they are still soft. Really weird part is that I have 20 blocks in this group and some are soft and others are just fine. Tried changing angle, new blade nothing helps. When I finally manage to get a decent section and pick it up on the slide, the OCT around the tissue want even pick up smooth it wrinkles really bad. I tried just a block of OCT that I froze on a chuck and it cut great. I am clueless as to what is going on. Samples were frozen by someone else and brought to me to be cut. Donna Reynolds Core IHC Lab M.D. Anderson Cancer Center, Houston TX Research lab 713-192-8106. Donna Reynolds Core IHC Lab Dept. Cancer Biology, SRB 1.660 713-792-8106 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Patrick Laurie HT(ASCP)QIHC CellNetix Pathology Laboratories 1124 Columbia Street, Suite 200 Seattle, WA 98104 plau...@cellnetix.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Help with OCT problem
I agree with previous responders to this query, as I've gotten alcohol on my blocks and since alcohol doesn't freeze real well it makes a sticky mess with the OCT. The affected OCT can be scraped off with a razor blade. Hopefully the tissue itself did not come in contact with alcohol prior to embedding. Regards, Merced --On Thursday, February 10, 2011 12:51 PM -0800 Patrick Laurie foreig...@gmail.com wrote: Donna, I would also check to make sure that the tissue wasn't held in an something with a lot of alcohol, like RNA later. I found out first hand that it doesn't work well. Good luck On Thu, Feb 10, 2011 at 11:38 AM, Reynolds,Donna M dreyn...@mdanderson.org wrote: Has anyone ever experienced OCT blocks that cut like they are soft. I have turned the cyrostat down 10 degrees lower than usual and they are still soft. Really weird part is that I have 20 blocks in this group and some are soft and others are just fine. Tried changing angle, new blade nothing helps. When I finally manage to get a decent section and pick it up on the slide, the OCT around the tissue want even pick up smooth it wrinkles really bad. I tried just a block of OCT that I froze on a chuck and it cut great. I am clueless as to what is going on. Samples were frozen by someone else and brought to me to be cut. Donna Reynolds Core IHC Lab M.D. Anderson Cancer Center, Houston TX Research lab 713-192-8106. Donna Reynolds Core IHC Lab Dept. Cancer Biology, SRB 1.660 713-792-8106 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Patrick Laurie HT(ASCP)QIHC CellNetix Pathology Laboratories 1124 Columbia Street, Suite 200 Seattle, WA 98104 plau...@cellnetix.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Merced M Leiker Research Technician III Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA lei...@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] CPT code 88363
You charge the attorney's office out the waazoo!!! WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of DANIEL HEWITT Sent: Thursday, February 10, 2011 3:25 PM To: Richard Cartun; Mike Pence; Histonet; Thomas Podawiltz Subject: RE: [Histonet] CPT code 88363 How about pulling blocks and cutting blank slides for an attorney who does not represent our hospital? We don't have to do this very much but it does come up. Thanks Daniel Hewitt Histology Supervisor, HVS 412-749-7371 This email, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, or an agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and delete and destroy all copies of the original message, including attachments. Please note that any views or opinions presented in this e-mail are solely those of the author and do not necessarily represent those of Heritage Valley Health System. The integrity and security of this message cannot be guaranteed on the internet. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Thursday, February 10, 2011 11:23 AM To: Mike Pence; Histonet; Thomas Podawiltz Subject: RE: [Histonet] CPT code 88363 I think so. Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax Mike Pence mpe...@grhs.net 2/10/2011 9:19 AM Is this a code that can be used even if you have your molecular done somewhere else? Mike -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Podawiltz, Thomas Sent: Wednesday, February 09, 2011 2:43 PM To: Richard Cartun; Histonet Subject: RE: [Histonet] CPT code 88363 It is my understanding that it used for examaination and selection of retrieved archival (ie,previously diagnosed) tissue for molecular analysis (eg, KRAS mutational analysis). page 440 of the 2011 AMA CPT , professional edition. Tom Podawiltz, HT (ASCP) Histology Section Head/Laboratory Safety Officer LRGHealthcare 603-524-3211 ext: 3220 From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Richard Cartun [rcar...@harthosp.org] Sent: Wednesday, February 09, 2011 1:12 PM To: Histonet Subject: [Histonet] CPT code 88363 Is anyone using the above CPT code for reviewing old pathology slides prior to ordering IHC testing or can it only be used for slide review prior to ordering molecular testing? Thanks. Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Help with OCT problem
Same with ETOH. We wipe our surfaces down with it to clean them so no cross contamination as we freeze our skin here on the metal side areas of our cryostats. If the alcohol hasn't been wiped dry or evaporated we have that problem too. Claire From: histonet-boun...@lists.utsouthwestern.edu on behalf of Liz Chlipala Sent: Thu 2/10/2011 1:49 PM To: Reynolds,Donna M; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Help with OCT problem Donna I have seen soft or rather sticky OCT samples in the past. If you freeze in isopentane and don't let the isopentane evaporate off the samples prior to wrapping the sample in foil, that excess isopentane changes the OCT, it makes it sticky. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
