[Histonet] in situ for mRNA - refresher course?
Hi all, after more than a decade away from the bench I have been asked to start doing ISH on cryosections for BMPs and TGFbetas (mRNA) . I desperately need some guidance - even an offer to training me. i don't even know waht type of probes have been developed in the interim. I am sure I can get some funding for a couple of days overseas.. Of course, vendors with local representation are welcome to contact me. I look forward to a HUMUNGOUS response regards -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel fax) 073 5574456 (emergencies only) There are nights when the wolves are silent and only the moon howls. George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] placental tissue
Dear collegues, I want to fixate placental tissue. What is the best solution to use? Formaldehyde 4%? neutral-buffered 10% formal saline? Other? Are there some tips and tricks while working with placental tissue? Thanks for your help. Regards Dr An Eerdekens University Hospitals Leuven Belgium ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] peggy wenk comments on HT/HTL practical
I was required to know and understand all this as well. I often have had to perform various aspects on an interview, and had no problems with this. Joelle Joelle Weaver MAOM, BA, (HTL) ASCP From: b-freder...@northwestern.edu To: lpw...@sbcglobal.net; mad...@verizon.net; histonet@lists.utsouthwestern.edu Date: Mon, 29 Aug 2011 12:35:50 + Subject: RE: [Histonet] peggy wenk comments on HT/HTL practical CC: I remember having to know, regarding Peggy's comment on why a reagent was on a piece of tissue, for my HTL what was going in every step of the retic (oxidation,reduction,toning etc) and believe me it was on the exam. We were taught to know the why from the techs that trained us and had taken the exam. If you chose to ignore what they said, it was on your head. As to the microtomy during an interview, I'm all for it as I have done it in the past (as an interviewer and interviewee) and most recently, as we had a tech come in from Romania and how were we to know what she knew? Their program is a CLS degree and she chose histo from that. Great tech by the way- histo is not much different the world over, from what I can see. Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lee Peggy Wenk Sent: Sunday, August 28, 2011 6:41 PM To: mad...@verizon.net; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] peggy wenk comments on HT/HTL practical Now add in a few other things, that I didn't directly mention in the original 2006 HistoNet reply. How many people are now using: - automated HE stainers - automated special stainers, including IHC stainer - automated coverslippers - automated microtomes - disposable blades Can you see why nearly everyone passed the practical? If they could cut a half-way decent section (with better microtomes and blades, easier to do), just put the slide on a machine and let the machine stain it. To fail, they basically had to NOT follow a LOT of directions, such as submitting colon for small intestine, submitting autolyzed gall bladder, doing the wrong stain (like doing a Prussian blue for iron, instead of the requested colloidal iron), grossing the tissue too small, microtoming too thick, putting the institution's name on the label, etc. Automation makes it easier to produce better sections and better stains, particularly if someone is a mediocre tech to begin with. As to whether the person understands the theory when using automated stainers - well, the fact that many of the people submitting the practical could pass the practical but would fail the written - that has been going on since ancient times, when staining was done by hand. They could follow the directions, but didn't know the reasons. Pour on solution A for 5 minutes, pour it off, pour on solution B for 10 minutes, rinse it off, and it's done. No idea what is in solution A or B, or what chemicals are binding to what components in the tissue, etc. Whether someone is doing the stain by hand or by machine, it's up to the person to have the curiosity to find out what is going on. Some people don't have it, and don't feel the urge to learn. That's one of the reasons I like going to state and national meetings, and reading HistoNet. These people WANT to learn! Hurrah for them! And there are a lot of people in the histology community willing to help people who WANT to learn - answering HistoNet, giving talks at state and national meetings, being a mentor, etc. Hurrah for them too! Peggy A. Wenk, HTL(ASCP)SLS Beaumont Health Systems Royal Oak, MI 48073 The above are my opinions and not those of my institution. -Original Message- From: mad...@verizon.net Sent: Sunday, August 28, 2011 2:23 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] peggy wenk comments on HT/HTL practical The dropping of the practical was explained so well by Peggy Wenk. I never got into the debate(glad I did not after reading Peggys comments), I would have argued to keep it. That said, indeed it is outdated. The fact that we were the last to do it I guess speaks volumes. I did struggle getting tissues for it for sure. Modern day, modern thinking. Thanks for the information Peggy and of course all you do fo this field. Nick(Rocky) Madary, HT/HTL(ASCP)QIHC ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Returning to Histology-Another Idea Won't Work
With all due respect and regard that should be afforded to the indisputable value of experience ( in time spans of 10 years or more), I would add that I do not see how this could be exchanged for academics.To me they are different animals- both adding something to the knowledge base of the person ( and speaking from my perspective of having both under my belt), and from my experience they are beneficial and useful to me, though in very different ways and different situations. I do not have the routes in front of me, and don't spend the time reviewing them that I formerly did, since I am not teaching at the moment, but I do not recall any experience level for any route stipulating 10 years? Joelle Weaver MAOM, BA, (HTL) ASCP From: histot...@imagesbyhopper.com Date: Sat, 27 Aug 2011 20:06:18 -0400 To: lpw...@sbcglobal.net Subject: Re: [Histonet] Returning to Histology-Another Idea Won't Work CC: histonet@lists.utsouthwestern.edu; araniqks...@yahoo.com 10 years experience to qualify for taking the exam? Is that an ASCP requirement? It sounds like they might be exchanging experience for the bachelor's degree? Have you checked out the online courses? I believe there are some that offer the bachelor's degree. Michelle Sent from my iPhone On Aug 27, 2011, at 1:45 PM, Lee Peggy Wenk lpw...@sbcglobal.net wrote: It looks like the time limits prohibit me from trying this, since I did it so long ago What is IT? What is prohibiting you from taking the HTL exam? What does having taken and passed the HT exam have to do with taking the HTL exam? Sorry, I'm really lost as to what you need, and how to help you. Peggy A. Wenk, HTL(ASCP)SLS Beaumont Hospital Royal Oak, MI 48073 The above response reflects my opinion, and not the hospital's view. -Original Message- From: Paula Sent: Thursday, August 25, 2011 3:31 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Returning to Histology-Another Idea Won't Work I thought it might be good to study and try for the HTL (already have school for HT and am certified) but it looks like the time limits prohibit me from trying this, since I did it so long ago. Anyone else have any ideas? I'd gladly buy the books and study. Cannot get into a lab here in NC without current experience. The closest school is in western NC, about 5 hours away. I didn't see anything near me in Raleigh. I was going to purchase the textbooks but now I see this won't work either: Histotechnologist, HTL(ASCP) Application Fee: $210 To be eligible for this examination category, an applicant must satisfy the requirements of at least one of the following routes: Route 1: Baccalaureate degree from a regionally accredited college/university with a combination of 30 semester hours (45 quarter hours) of biology and chemistry AND successful completion of a NAACLS accredited Histotechnician or Histotechnology program within the last 5 years; OR Route 2: Baccalaureate degree from a regionally accredited college/university with a combination of 30 semester hours (45 quarter hours) of biology and chemistry AND one year full time acceptable experience in a histopathology (clinical, veterinary, industry or research) laboratory in the U.S., Canada or an accredited laboratory* within the last ten years. *laboratory accredited by a CMS approved accreditation organization (i.e., AABB, CAP, COLA, DNV, The Joint Commission, etc.) Clinical Laboratory Experience To fulfill the experience requirement for the Histotechnologist examination, you must have experience, within the last ten years, in the following areas: Fixation Microtomy Processing Staining Any ideas? I have posted this before, but I keep on trying! Paula ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] harrasment for humble histotechs
I think that I have worked with some of these people, like I posted previously, if you don't commit the effort to learn and perfect your technique and understanding when you are learning and a student, and try to do a work around- short cut, by cheating, IT WILL SHOW at some point. This may not catch up with you that day, or even the next, but in a specific lab or market, people get to know pretty quickly and easily who is really committed to doing quality work , who knows the how and why of what they are doing, and who just shows up for a job... Joelle Weaver MAOM, BA, (HTL) ASCP From: histot...@imagesbyhopper.com Date: Sat, 27 Aug 2011 16:50:26 -0400 To: mad...@verizon.net Subject: Re: [Histonet] harrasment for humble histotechs CC: histonet@lists.utsouthwestern.edu :o). I'm not even sure I could find my results after all these years and 9 moves! I *do* remember how picture perfect those slides had to be though. Tiny air bubble=graded down. Folds=forget it. Knife marks=bad. I did both the HT and the HTL practicals and now new students don't even have to submit them. I didn't cheat, but over the years I saw students attempting to cheat. If I saw them, I would remind them that they were to work independently, but who knows what they did when I wasn't looking? Integrity is what you do when no one is looking. Sent from my iPhone On Aug 27, 2011, at 3:25 PM, mad...@verizon.net wrote: Gentle harrassment. I encourage you to look at you scores for your practical and written exam. Between gigs I am getting my important documents together and found my old scores for the HTL, no thte HT, however, both parts of the exam were taken by yours truly. Never got into the talk of practical vs no practical. I do think it si a good idea to keep it. Honest techs will be proud to do a practical. Looking at scores made me feel good that the next great job is right around the corner. Nick(Rocky) Madary, HT/HTL(ASCP)QIHC ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Moh's Tech Opening in Cleveland
We are currently looking for a Moh's Tech brand new graduate or with experience. Position is with a large Dermatology Practice in Cleveland with 6 offices in the Greater Cleveland Area. The position involves some travel between offices as well as working 1-2 days in their Dermatopathology lab. 3-4 days the candidate would be cutting tissue for the same physician mostly. Please contact me today if you might know anyone interested! Thanks! Brian Feldman Principal Prometheus Healthcare Office 301-693-9057 Fax 301-368-2478 http://us.mc538.mail.yahoo.com/mc/compose?to=br...@prometheushealthcare.com br...@prometheushealthcare.com http://www.prometheushealthcare.com/ www.prometheushealthcare.com *** Stay up to date on the newest positions and healthcare trends nationwide on Twitter!*** http://twitter.com/PrometheusBlog http://twitter.com/PrometheusBlog ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] troubleshooting Oil Red O
Hi all, I'm using the Churukian method posted by Gayle Callis a few years ago and I have encountered a strange problem. The lipid stains fine, and the Gill's hematoxylin looks good before I coverslip with Vectashield Hard Set aqueous mounting medium. But within a matter of minutes, the hematoxylin leaches out. I popped off the coverslip and tried again with Mayer's hematoxylin with the same results. I have used Vectashield Hard Set before, in fact I pulled a 5 year old control that still looks great. Does anyone have any ideas? Denise Crowley Histology Facility Manager Koch Center for Integrative Cancer Research Massachusetts Institute of Technology 500 Main St. 76-182 Cambridge MA 02139 617-258-8183 dencr...@mit.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] excellent condition used embedding center
Im looking for an excellent conditions embbeding center Lcda. Mary V. Guerrero,BS, MBA,HtL Administradora/Coordinadora General Pathology Lab. 55 N. Dr. Basora Edificio Médico IV Oficina 206 Mayaguez, Puerto Rico 00680 Tel. 787-834-8202 Fax: 787-831-5255 Sra. Dimary Valentín Secretaria Area de Facturación Advertencia de Confidencialidad: Esta comunicación, incluyendo los escritos que le acompañan, es para conocimiento y uso exclusivo del destinatario y esta protegida por la LEY FEDERAL HIPAA Y LEY ESTATAL 194 CONSTITUYENDOLO UN DOCUMENTO LEGAL Y PRIVILEGIADO. Si por error usted lo recibe y no es el destinatario ni a sido autorizado para leer este documento,SE LE ADVIERTE QUE ESTA ESTRICTAMENTE PROHIBIDO FOTOCOPIAR, REVELAR, DISTRIBUIR O TOMAR ACCION ALGUNA SOBRE EL CONTENIDO DE ESTA TRANSMISION ELECTRONICA. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] troubleshooting Oil Red O
It may be that Vector has changed the formulation for Hard Set. I suggest you call them up. I have found their technical support to be excellent. Geoff On 8/29/2011 10:30 AM, Denise G Crowley wrote: Hi all, I'm using the Churukian method posted by Gayle Callis a few years ago and I have encountered a strange problem. The lipid stains fine, and the Gill's hematoxylin looks good before I coverslip with Vectashield Hard Set aqueous mounting medium. But within a matter of minutes, the hematoxylin leaches out. I popped off the coverslip and tried again with Mayer's hematoxylin with the same results. I have used Vectashield Hard Set before, in fact I pulled a 5 year old control that still looks great. Does anyone have any ideas? Denise Crowley Histology Facility Manager Koch Center for Integrative Cancer Research Massachusetts Institute of Technology 500 Main St. 76-182 Cambridge MA 02139 617-258-8183 dencr...@mit.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- -- ** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcaul...@umdnj.edu ** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] iliac artery attachment to slides?
Good morning everybody!! I am trying to attach iliac arteries embedded in MMA (methyl methacrylate + dibutyl phthalate + perkadox 16) to gelatin coated slides to allow us to stain them with the Movat's Pentachrome. Unfortunately, I have a real problem getting them to attach - shortly after they are placed into 2-MEA for plastic removal, the section detaches from the slide. I have mounted hundreds of sections to slides before with great success, but have never tried iliac arteries before. When mounting them using the conventional method (a drop of 50% ETOH, roll them onto the slide using a plastic coverslip, clamping the stack and placing it into a 45 - 50 degree Celcius oven for 24 to 48 hours), the sections attach beautifully and do not fall off during deplasticization. The problem is that they are completely consumed with wrinkles around the entire circular area of the artery, rendering them unuseable. I have tried dry mounting (without using 50% ETOH or dH2O), I have tried floating the section onto a slide from a warm ETOH or dH2O solution (in water bath), I have tried using a slide warmer, I have tried a heat gun at low and high temperature settings, etc., etc.. The sections look great using the dry mounting methods (i.e. they don't wrinkle), but they detach from the slide very quickly following submersion in 2-MEA. It appears to me that the wrinkles appear following the introduction of the ETOH or dH2O. If anyone would have any suggestions or comments, it would be greatly appreciated. Thanks again for all of your help. Have a great day!! Jim ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] lot to lot
Good Afternoon I would like to get an idea of how many slides people are running for lot to lot antibody and detection testing. For example would a tonsil with good internal negative and positive elements be good enough to validate a new lot of LCA? Thank you Mary ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] lot to lot
I think yes! René J. --- On Mon, 8/29/11, Mary Helie mary.he...@yale.edu wrote: From: Mary Helie mary.he...@yale.edu Subject: [Histonet] lot to lot To: histonet@lists.utsouthwestern.edu Date: Monday, August 29, 2011, 12:06 PM Good Afternoon I would like to get an idea of how many slides people are running for lot to lot antibody and detection testing. For example would a tonsil with good internal negative and positive elements be good enough to validate a new lot of LCA? Thank you Mary ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] lot to lot
I know different people do lot to lot checks in various ways. We use concentrated antibodies, and so we like to do a 'sandwich' titer check on the new lot using our usual tissue control tissue. For example, if the last CD20 lot was at 1:100 we would check the new lot at 1:50, 1:100, 1:200 using tonsil tissues our standard SOPs. I did have 1 inspector tell me they thought that was unnecessary. It's our preference to do 3 titers assess the maximum performance in one fell swoop. Our detection lot to lot check is completely different. We check 8-10 different antibodies using our standard control tissue with the new detection lot. we use antibodies with a variety of cellular expressions to assess cytoplasmic, membranous nuclear expression. We make sure to include some of our 'fussy' antibodies. Of course we have SOPs forms to go along with this. Call me crazy - I prefer to think of it as meticulous dedicated! We have had a couple of problems with detections over the years. Just my 2 cents. Patti Ann Loykasek HTL, QIHC PhenoPath Laboratories On Aug 29, 2011, at 9:06 AM, Mary Helie wrote: Good Afternoon I would like to get an idea of how many slides people are running for lot to lot antibody and detection testing. For example would a tonsil with good internal negative and positive elements be good enough to validate a new lot of LCA? Thank you Mary ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Histotech position
Histonet members, York Hospital in York Pennsylvania is looking for a histotech to work day shift Monday through Friday with an occasional 5 hours on Saturday. If you are interested, apply at www.wellspan.orghttp://www.wellspan.org or contact: Andrea Bilger Team Leader, Histology York Hospital 1001 South George St. York, Pa. 17405 717-851-5040 CONFIDENTIALITY NOTICE: This email may contain confidential health information that is legally privileged. This information is intended for the use of the named recipient(s). The authorized recipient of this information is prohibited from disclosing this information to any party unless required to do so by law or regulation and is required to destroy the information after its stated need has been fulfilled. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or action taken in reliance on the contents of this email is strictly prohibited. If you receive this e-mail message in error, please notify the sender immediately to arrange disposition of the information. . __ This e-mail has been scanned by MCI Managed Email Content Service, using Skeptic(tm) technology powered by MessageLabs. For more information on MCI's Managed Email Content Service, visit http://www.mci.com. __ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Thanks!
Thank you all- this was very helpful. I appreciate it. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Cost of an HE
Hi Netters, If anyone has figured the cost of an HE lately will you pleas share the info with me? It has been awhile since I have done this. My email is below if you do not mind sharing. Thank you in advance. Carole Carole Fields, HT (ASCP) Histology Supervisor Northside Hospital Atlanta, GA 30342 carol.fie...@northside.com CONFIDENTIALITY NOTICE: This electronic mail transmission has been sent by Northside Hospital. It may contain information that is confidential, privileged, proprietary, or otherwise legally exempt from disclosure. If you are not the intended recipient, you are hereby notified that you are not authorized to read, print, retain, copy or disseminate this message, any part of it, or any attachments. If you have received this message in error, please delete this message and any attachments from your system without reading the content and notify the sender immediately of the inadvertent transmission. There is no intent on the part of the sender to waive any privilege. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: iliac artery attachment to slides?
Hi Jim, sounds like you are having a time of it. I figure Jack Ratliff will chime in as soon as he sees this. In the meantime I will give you the same advice he gave to me. If you are having troubles with tissues adhering, try Haupt's adhesive. You can find recipes on the internet to make it yourself, or you can buy it commercially ready to use (www.dornandhart.com). I have heard from several people who use this consistently with their MMA and they swear by it. Have you tried stretching the sections using a few drops of 50% alcohol and a couple of soft brushes prior to covering in plastic and clamping? It's going to be tough getting circular tissues wrinkle free. I hope others with more experience than me will chime in on this. Best wishes, Teri Johnson, HT(ASCP)QIHC Head, Histology and Electron Microscopy Stowers Institute for Medical Research Kansas City, MO ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Cost of an HE
The information on direct costs is in separate e-mail René J. --- On Mon, 8/29/11, Carol Fields carol.fie...@northside.com wrote: From: Carol Fields carol.fie...@northside.com Subject: [Histonet] Cost of an HE To: histonet@lists.utsouthwestern.edu Date: Monday, August 29, 2011, 2:04 PM Hi Netters, If anyone has figured the cost of an HE lately will you pleas share the info with me? It has been awhile since I have done this. My email is below if you do not mind sharing. Thank you in advance. Carole Carole Fields, HT (ASCP) Histology Supervisor Northside Hospital Atlanta, GA 30342 carol.fie...@northside.com CONFIDENTIALITY NOTICE: This electronic mail transmission has been sent by Northside Hospital. It may contain information that is confidential, privileged, proprietary, or otherwise legally exempt from disclosure. If you are not the intended recipient, you are hereby notified that you are not authorized to read, print, retain, copy or disseminate this message, any part of it, or any attachments. If you have received this message in error, please delete this message and any attachments from your system without reading the content and notify the sender immediately of the inadvertent transmission. There is no intent on the part of the sender to waive any privilege. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Charcoal filters for VIP1000 K series
There are two charcoal filters (#6160) available for a Sakura Finetek VIP 1000, K series tissue processor. These are free for the lab who can use them. Contact Maria Jerome [mjerome.mt...@gmail.com] for shipping arrangements. She is not on Histonet. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] training techs
Way to go Patsy!! :o)
Sent from my iPhone
On Aug 29, 2011, at 5:36 PM, Patsy Ruegg pru...@ihctech.net wrote:
Hey Everyone,
The third tech I trained in the last 3 years just passed her HTL exam last
Friday, that all makes me feel pretty good. I just signed an affiliate
agreement to be a clinical training site for the Histology program at UND
today, they are sending me another student who will be with me for 2
semesters.
Regards,
Patsy
Patsy Ruegg, HT(ASCP)QIHC
IHCtech, LLC
Fitzsimmons BioScience Park
12635 Montview Blvd. Suite 215
Aurora, CO 80010
P-720-859-4060
F-720-859-4110
wk email mailto:pru...@ihctech.net pru...@ihctech.net
web site http://www.ihctech.net www.ihctech.net
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Re: [Histonet] peggy wenk comments on HT/HTL practical
Interestingly, I have never been required to section tissue in a job interview. I have worked at four hospitals, three private labs and one research facility (hubby moved us around a bit!) I just recently hired two techs, directly out of school with no real world experience. I did ask them to cut some slides for me! ;o) Michelle Sent from my iPhone On Aug 29, 2011, at 9:05 AM, joelle weaver joellewea...@hotmail.com wrote: I was required to know and understand all this as well. I often have had to perform various aspects on an interview, and had no problems with this. Joelle Joelle Weaver MAOM, BA, (HTL) ASCP From: b-freder...@northwestern.edu To: lpw...@sbcglobal.net; mad...@verizon.net; histonet@lists.utsouthwestern.edu Date: Mon, 29 Aug 2011 12:35:50 + Subject: RE: [Histonet] peggy wenk comments on HT/HTL practical CC: I remember having to know, regarding Peggy's comment on why a reagent was on a piece of tissue, for my HTL what was going in every step of the retic (oxidation,reduction,toning etc) and believe me it was on the exam. We were taught to know the why from the techs that trained us and had taken the exam. If you chose to ignore what they said, it was on your head. As to the microtomy during an interview, I'm all for it as I have done it in the past (as an interviewer and interviewee) and most recently, as we had a tech come in from Romania and how were we to know what she knew? Their program is a CLS degree and she chose histo from that. Great tech by the way- histo is not much different the world over, from what I can see. Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lee Peggy Wenk Sent: Sunday, August 28, 2011 6:41 PM To: mad...@verizon.net; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] peggy wenk comments on HT/HTL practical Now add in a few other things, that I didn't directly mention in the original 2006 HistoNet reply. How many people are now using: - automated HE stainers - automated special stainers, including IHC stainer - automated coverslippers - automated microtomes - disposable blades Can you see why nearly everyone passed the practical? If they could cut a half-way decent section (with better microtomes and blades, easier to do), just put the slide on a machine and let the machine stain it. To fail, they basically had to NOT follow a LOT of directions, such as submitting colon for small intestine, submitting autolyzed gall bladder, doing the wrong stain (like doing a Prussian blue for iron, instead of the requested colloidal iron), grossing the tissue too small, microtoming too thick, putting the institution's name on the label, etc. Automation makes it easier to produce better sections and better stains, particularly if someone is a mediocre tech to begin with. As to whether the person understands the theory when using automated stainers - well, the fact that many of the people submitting the practical could pass the practical but would fail the written - that has been going on since ancient times, when staining was done by hand. They could follow the directions, but didn't know the reasons. Pour on solution A for 5 minutes, pour it off, pour on solution B for 10 minutes, rinse it off, and it's done. No idea what is in solution A or B, or what chemicals are binding to what components in the tissue, etc. Whether someone is doing the stain by hand or by machine, it's up to the person to have the curiosity to find out what is going on. Some people don't have it, and don't feel the urge to learn. That's one of the reasons I like going to state and national meetings, and reading HistoNet. These people WANT to learn! Hurrah for them! And there are a lot of people in the histology community willing to help people who WANT to learn - answering HistoNet, giving talks at state and national meetings, being a mentor, etc. Hurrah for them too! Peggy A. Wenk, HTL(ASCP)SLS Beaumont Health Systems Royal Oak, MI 48073 The above are my opinions and not those of my institution. -Original Message- From: mad...@verizon.net Sent: Sunday, August 28, 2011 2:23 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] peggy wenk comments on HT/HTL practical The dropping of the practical was explained so well by Peggy Wenk. I never got into the debate(glad I did not after reading Peggys comments), I would have argued to keep it. That said, indeed it is outdated. The fact that we were the last to do it I guess speaks volumes. I did struggle getting
[Histonet] RE: Going for the HTL
To Paula: Who wrote, thought it might be good to study and try for the HTL (already have school for HT and am certified) but it looks like the time limits prohibit me from trying this, since I did it so long ago. Anyone else have any ideas? I'd gladly buy the books and study. Cannot get into a lab here in NC without current experience. The closest school is in western NC, about 5 hours away. I didn't see anything near me in Raleigh. I was going to purchase the textbooks but now I see this won't work either: There is an internet program just down the road in Charleston for HTLs: www.musc.edu/histoprogram Our program is NACCLS approved, has start dates in April and Sept. You can also call the director, Karen Brinker Geils @ (843) 792-4013. Take a look, I'll bet you can do it! It took me over 10 years, but I got my BS and passed my HTL just this year! Life got in my way for a while, but I did it, and I know you can too! Wanda Shotsberger Gray HT/HTL (ASCP), QIHC ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: peggy wenk comments on HT/HTL practical
I really appreciate Peggy Wenk's analysis of the practical examination and why it had to be dropped. I never really understood the issue before. I must confess I always enjoyed helping the prospective examinee obtain exactly the right tissue. No, this endometrium is poorly preserved. We'll arrange with surgery for a completely fresh specimen - I'll block it initially for the diagnosis, then we'll fix it overnight and then block it exactly to specifications. - Ick - this one's been curetted - we'll get another one. I'll block the margins of this colon resection specimen, then we'll pin a portion of tissue onto paraffin and fix it flat overnight. Next time I do an autopsy we'll get a lumbar spinal cord in the intact dura. I'll open the dura dorsally and ventrally with iridectomy scissors, then we'll hang it in neutral buffered formalin for two days. Then I'll tie the dura and dependent nerves with a cotton string. When you embed you'll remove the string, taking care that dura and nerves remain in position. After that it's all yours. If it doesn't work the first time, we've got three more levels in the jar. OK, I'm a geek, I'm 72 years old, I got a right. Bob Richmond Samurai Pathologist Knoxville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
