[Histonet] Fw:
Greetings, friend! http://morcego.ind.br/response.php?ezoponiperufiv=97edawiho=258afuhaf=52 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Diamond Knives
Hi Philip, Diatome is the brand we use. Lately they have been having resharpening issues. We have had to send several back after resharpening because the knives left scratch marks on the thin sections. That said, they are friendly and helpful and have never complained about us returning one for additional resharpening. There are other brands on the market, I have not used them in a while so I cannot comment about those vendors. If you have anyother questions, feel free to ask. Paula :-) -- Paula Sicurello, HTL (ASCP) Supervisor, Electron Microscope Laboratory Duke University Health System Rm.#251M, Duke South, Green Zone Durham, North Carolina 27710 P: 919.684.2091 On Wed, Mar 14, 2012 at 10:42 PM, Philip Slakmon slak...@yahoo.com wrote: Good Afternoon, I was interested in knowing people's opinions on the different diamond knives on the market. Opinions could be based on quality, design, workmanship, delivery, customer service/support, pricing, Thank you, Philip ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] PAS Diastase
No, you are correct. The tech should always give the pathologists one with and another without diastase. René J. --- On Thu, 3/15/12, Jennifer MacDonald jmacdon...@mtsac.edu wrote: From: Jennifer MacDonald jmacdon...@mtsac.edu Subject: [Histonet] PAS Diastase To: histonet@lists.utsouthwestern.edu Date: Thursday, March 15, 2012, 1:47 AM Hi All, At a local lab when a pathologist orders a PAS diastase the histotechnicians do just one slide with diastase. They do not do an undigested slide. How would the pathologist know if the digested slide had a glycogen to begin with? Am I over thinking this? Thanks, Jennifer ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Diamond Knives
Have a look at Delaware Diamond Knives (DDK). Their website I believe is www.ddk.com. I'm sure they can help you or at a minimum point you in the right direction. Best Regards, Jack On Mar 14, 2012, at 9:42 PM, Philip Slakmon slak...@yahoo.com wrote: Good Afternoon, I was interested in knowing people's opinions on the different diamond knives on the market. Opinions could be based on quality, design, workmanship, delivery, customer service/support, pricing, Thank you, Philip ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] PAS Diastase
I agree with Rene. A control (undigested) slide is necessary. Geoff On 3/15/2012 1:47 AM, Jennifer MacDonald wrote: Hi All, At a local lab when a pathologist orders a PAS diastase the histotechnicians do just one slide with diastase. They do not do an undigested slide. How would the pathologist know if the digested slide had a glycogen to begin with? Am I over thinking this? Thanks, Jennifer ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- -- ** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcaul...@umdnj.edu ** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Diamond Knives
Philip, We also use Dorn and Hart Microedge. As far as I know they only do Tungsten Carbide and steel knives but if that is what your looking for they are the best priced, quality, and easiest to work with. Marc On Thursday, March 15, 2012, Bernice Frederick b-freder...@northwestern.edu wrote: We have a DDK knife, works great on undecalcified bone. We send it to Dorn and Hart Microedge for resharpening as they are local to us and have been at it for years. They used to sharpen our old microtome knives. Talk about dating yourself! Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto: histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jack Ratliff Sent: Thursday, March 15, 2012 8:20 AM To: Philip Slakmon Cc: Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Diamond Knives Have a look at Delaware Diamond Knives (DDK). Their website I believe is www.ddk.com. I'm sure they can help you or at a minimum point you in the right direction. Best Regards, Jack On Mar 14, 2012, at 9:42 PM, Philip Slakmon slak...@yahoo.com wrote: Good Afternoon, I was interested in knowing people's opinions on the different diamond knives on the market. Opinions could be based on quality, design, workmanship, delivery, customer service/support, pricing, Thank you, Philip ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Vacuum processing question
Hello all, I have a question about vacuum processing. We have ligament tissues that are processed over night, then are to be vacuum processed. I've never done this before, but was able to get ahold of a vacuum oven. From what I understand, I just put the tissues in a metal container and turn on the vacuum oven for 1 hour. But what are they supposed to be in? Parafin? Alcohol? Do I just leave them in the cassettes and not put them in any sort of liquid? Is there a specific temperature or vacuum that any of you have used that works particularly well? Also, is it possible to let the tissues sit over the weekend after the regular processing before putting them in the vacuum oven for processing? Any advice would be greatly appreciated as I am new to this world of histology, having a biochemistry background. Many thanks in advance, Kara ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Processor Preferences??
Hello Jennifer, I saw your message about tissue processors on the Histonet Archive May 2006 and wondered which unit you decided to purchase? We are looking for one now and have three in mind, Thermo Pathcentre, Leica ASP300s and the Tissue-Tek VIP6. Your thoughts on your choice and on these listed. Thanks you, John John A. Baker The University of Michigan Orthopaedic Research Laboratories Histology Unit 109 Zina Pitcher Place, 2218 BSRB Ann Arbor, MI 48109-2200 734-936-1635 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Processor Preferences??
Of the 3 you mention, I would buy VIP6. Let's see what Jennifer answers. René J. --- On Thu, 3/15/12, John Baker bak...@umich.edu wrote: From: John Baker bak...@umich.edu Subject: [Histonet] Processor Preferences?? To: histonet@lists.utsouthwestern.edu Date: Thursday, March 15, 2012, 10:13 AM Hello Jennifer, I saw your message about tissue processors on the Histonet Archive May 2006 and wondered which unit you decided to purchase? We are looking for one now and have three in mind, Thermo Pathcentre, Leica ASP300s and the Tissue-Tek VIP6. Your thoughts on your choice and on these listed. Thanks you, John John A. Baker The University of Michigan Orthopaedic Research Laboratories Histology Unit 109 Zina Pitcher Place, 2218 BSRB Ann Arbor, MI 48109-2200 734-936-1635 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Processor Preferences??
If it was me and it is in the future, I want a Leica Peloris. Leica also has a new one that is a single chamber version of the Peloris. Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of John Baker Sent: Thursday, March 15, 2012 9:13 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Processor Preferences?? Hello Jennifer, I saw your message about tissue processors on the Histonet Archive May 2006 and wondered which unit you decided to purchase? We are looking for one now and have three in mind, Thermo Pathcentre, Leica ASP300s and the Tissue-Tek VIP6. Your thoughts on your choice and on these listed. Thanks you, John John A. Baker The University of Michigan Orthopaedic Research Laboratories Histology Unit 109 Zina Pitcher Place, 2218 BSRB Ann Arbor, MI 48109-2200 734-936-1635 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Vacuum processing question
Vacuum processing has been advocated in the past as a way to process, but the penetration of liquid is not a function of negative pressure (vacuum) but of positive pressure. In the initial experiments with the Peloris instrument in Australia (by BioSystems before it was bought by Leica Microsystems) they subjected the tissues that were dehydrated with ethanol to high temperature under vacuum because they needed to eliminate the ethanol before the hot paraffin wax infiltration because ethanol absolutely does not mix with paraffin. Once they changed to isopropyl alcohol (that mixes with melted paraffin) the vacuum and heat step was reduced in time and intensity. The VIP uses vacuum in their retort as the only way the have to empty the retort but that vacuum step has effect on the tissues inside only during the few minutes the retort is empty, that is when the tissues as subjected to vacuum. At that moment the liquids inside the tissues will exit them and facilitate the penetration of the next liquid that will enter the retort under pressure. Vacuum per se has no place in tissue processing except to facilitate the elimination of some liquid that has already penetrated the tissue and as an intermediate step between other steps involving a liquid. I just do not know why you expect to obtain with your vacuum oven. They are usually used when you want to evaporate some liquid at a temperature below the boiling point. In the same way that a pressure cooker allows you to heat water at above 100ºC without boiling, a vacuum oven will allow you to boil water at less than 100ºC. Both variations of the boiling point (above or below) are an inverse relation with the (+) or (-) pressure. If you leave your specimens without any liquid in your vacuum oven, you will just dehydrate them. It seems that you have been asked to do this without any further instructions. Ask whomever requested you to do this what for you are supposed to do this. Never fear to ask anybody who instructs you to do something: why! René J. --- On Thu, 3/15/12, Kara Lee karabo...@hotmail.com wrote: From: Kara Lee karabo...@hotmail.com Subject: [Histonet] Vacuum processing question To: histonet@lists.utsouthwestern.edu Date: Thursday, March 15, 2012, 10:10 AM Hello all, I have a question about vacuum processing. We have ligament tissues that are processed over night, then are to be vacuum processed. I've never done this before, but was able to get ahold of a vacuum oven. From what I understand, I just put the tissues in a metal container and turn on the vacuum oven for 1 hour. But what are they supposed to be in? Parafin? Alcohol? Do I just leave them in the cassettes and not put them in any sort of liquid? Is there a specific temperature or vacuum that any of you have used that works particularly well? Also, is it possible to let the tissues sit over the weekend after the regular processing before putting them in the vacuum oven for processing? Any advice would be greatly appreciated as I am new to this world of histology, having a biochemistry background. Many thanks in advance, Kara ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] CDC42
Does anyone have a good protocol for CDC42? The ab I am using if from santa cruz sc-8401. I stain on the dako autostainer plus using flex reagents. Kathryn Stoll, HT(ASCP) Department of Pathology Medical College of Wisconsin 9200 W Wisconsin Ave Milwaukee WI 53226 414.805.1525 kst...@mcw.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Processor Preferences??
John, Go for the ASP300S and then let's talk again at your convenience about resin infiltration needs. I hope the information I sent to you was both informative and helpful! Regards, Jack On Mar 15, 2012, at 9:13 AM, John Baker bak...@umich.edu wrote: Hello Jennifer, I saw your message about tissue processors on the Histonet Archive May 2006 and wondered which unit you decided to purchase? We are looking for one now and have three in mind, Thermo Pathcentre, Leica ASP300s and the Tissue-Tek VIP6. Your thoughts on your choice and on these listed. Thanks you, John John A. Baker The University of Michigan Orthopaedic Research Laboratories Histology Unit 109 Zina Pitcher Place, 2218 BSRB Ann Arbor, MI 48109-2200 734-936-1635 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Diamond Knives
Philip, Marc is correct in everything that he has stated, but if you are looking for diamond knife purchases or services as you indicated in your original posting, I am only aware of DDK providing this service. Best Regards, Jack Jack Ratliff Hard Tissue Histologist Chairman, Hard Tissue Committee - National Society for Histotechnology On Mar 15, 2012, at 9:10 AM, Marc DeCarlo boneima...@gmail.com wrote: Philip, We also use Dorn and Hart Microedge. As far as I know they only do Tungsten Carbide and steel knives but if that is what your looking for they are the best priced, quality, and easiest to work with. Marc On Thursday, March 15, 2012, Bernice Frederick b-freder...@northwestern.edu wrote: We have a DDK knife, works great on undecalcified bone. We send it to Dorn and Hart Microedge for resharpening as they are local to us and have been at it for years. They used to sharpen our old microtome knives. Talk about dating yourself! Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jack Ratliff Sent: Thursday, March 15, 2012 8:20 AM To: Philip Slakmon Cc: Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Diamond Knives Have a look at Delaware Diamond Knives (DDK). Their website I believe is www.ddk.com. I'm sure they can help you or at a minimum point you in the right direction. Best Regards, Jack On Mar 14, 2012, at 9:42 PM, Philip Slakmon slak...@yahoo.com wrote: Good Afternoon, I was interested in knowing people's opinions on the different diamond knives on the market. Opinions could be based on quality, design, workmanship, delivery, customer service/support, pricing, Thank you, Philip ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: PAS Diastase
I disagree that two patient slides are needed. What is needed for diagnosis is one patient slide with digestion, and two control slides, one with and one without. If the control slide shows adequate digestion of the glycogen and the other control slide shows adequate PAS positivity, the pathologist can be assured that if the patient slide has any PAS positivity it is not due to glycogen. I am guessing this is only in cases where they are not concerned with glycogen storage as part of the diagnosis (liver biopsy). Ask your pathologist what he or she is looking for. My guess is it is not glycogen, but some other PAS positive component. On 3/15/2012 1:47 AM, Jennifer MacDonald wrote: Hi All, At a local lab when a pathologist orders a PAS diastase the histotechnicians do just one slide with diastase. They do not do an undigested slide. How would the pathologist know if the digested slide had a glycogen to begin with? Am I over thinking this? Thanks, Jennifer Teri Johnson, HT(ASCP)QIHC GNF Histology Lab Manager Genomics Institute of the Novartis Research Foundation 858-332-4752 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Diamond Knives
Micro Star Technologies, Huntsville, Texas is also a supplier of diamond knives and a resharpening service. I have used them many times over the past 20+ years and have been satisfied with their product. Jean Mitchell, BS HT (ASCP) University of Wisconsin Hospital Clinics Neuromuscular Laboratory 600 Highland Avenue Madison, WI 53792-5132 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jack Ratliff Sent: Thursday, March 15, 2012 10:59 AM To: Marc DeCarlo Cc: Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Diamond Knives Philip, Marc is correct in everything that he has stated, but if you are looking for diamond knife purchases or services as you indicated in your original posting, I am only aware of DDK providing this service. Best Regards, Jack Jack Ratliff Hard Tissue Histologist Chairman, Hard Tissue Committee - National Society for Histotechnology On Mar 15, 2012, at 9:10 AM, Marc DeCarlo boneima...@gmail.com wrote: Philip, We also use Dorn and Hart Microedge. As far as I know they only do Tungsten Carbide and steel knives but if that is what your looking for they are the best priced, quality, and easiest to work with. Marc On Thursday, March 15, 2012, Bernice Frederick b-freder...@northwestern.edu wrote: We have a DDK knife, works great on undecalcified bone. We send it to Dorn and Hart Microedge for resharpening as they are local to us and have been at it for years. They used to sharpen our old microtome knives. Talk about dating yourself! Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jack Ratliff Sent: Thursday, March 15, 2012 8:20 AM To: Philip Slakmon Cc: Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Diamond Knives Have a look at Delaware Diamond Knives (DDK). Their website I believe is www.ddk.com. I'm sure they can help you or at a minimum point you in the right direction. Best Regards, Jack On Mar 14, 2012, at 9:42 PM, Philip Slakmon slak...@yahoo.com wrote: Good Afternoon, I was interested in knowing people's opinions on the different diamond knives on the market. Opinions could be based on quality, design, workmanship, delivery, customer service/support, pricing, Thank you, Philip ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: Subject: [Histonet] PAS Diastase
Jennifer, No you are not. Matt Lunetta BS HT(ASCP) Longmont United Hospital Message: 9 Date: Wed, 14 Mar 2012 22:47:17 -0700 From: Jennifer MacDonald Subject: [Histonet] PAS Diastase To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=US-ASCII Hi All, At a local lab when a pathologist orders a PAS diastase the histotechnicians do just one slide with diastase. They do not do an undigested slide. How would the pathologist know if the digested slide had a glycogen to begin with? Am I over thinking this? Thanks, Jennifer ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
AW: [Histonet] Re: PAS Diastase
It depends on the tissue-component to be shown. If you just want to see tissue-architecture or eg. in liver mallory-bodies and glycogen doesn't play a role, one slide would be sufficient. Gudrun On 3/15/2012 1:47 AM, Jennifer MacDonald wrote: Hi All, At a local lab when a pathologist orders a PAS diastase the histotechnicians do just one slide with diastase. They do not do an undigested slide. How would the pathologist know if the digested slide had a glycogen to begin with? Am I over thinking this? Thanks, Jennifer Teri Johnson, HT(ASCP)QIHC GNF Histology Lab Manager Genomics Institute of the Novartis Research Foundation 858-332-4752 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Diamond Knives
Philip, Try also Diatome Diamond Knives. http://www.diatomeknives.com/ Regards, Lita Duraine EM Technologist Bellen Lab HHMI- Molecular Genetics -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mitchell Jean A Sent: Thursday, March 15, 2012 12:07 PM To: Jack Ratliff Cc: Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Diamond Knives Micro Star Technologies, Huntsville, Texas is also a supplier of diamond knives and a resharpening service. I have used them many times over the past 20+ years and have been satisfied with their product. Jean Mitchell, BS HT (ASCP) University of Wisconsin Hospital Clinics Neuromuscular Laboratory 600 Highland Avenue Madison, WI 53792-5132 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jack Ratliff Sent: Thursday, March 15, 2012 10:59 AM To: Marc DeCarlo Cc: Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Diamond Knives Philip, Marc is correct in everything that he has stated, but if you are looking for diamond knife purchases or services as you indicated in your original posting, I am only aware of DDK providing this service. Best Regards, Jack Jack Ratliff Hard Tissue Histologist Chairman, Hard Tissue Committee - National Society for Histotechnology On Mar 15, 2012, at 9:10 AM, Marc DeCarlo boneima...@gmail.com wrote: Philip, We also use Dorn and Hart Microedge. As far as I know they only do Tungsten Carbide and steel knives but if that is what your looking for they are the best priced, quality, and easiest to work with. Marc On Thursday, March 15, 2012, Bernice Frederick b-freder...@northwestern.edu wrote: We have a DDK knife, works great on undecalcified bone. We send it to Dorn and Hart Microedge for resharpening as they are local to us and have been at it for years. They used to sharpen our old microtome knives. Talk about dating yourself! Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jack Ratliff Sent: Thursday, March 15, 2012 8:20 AM To: Philip Slakmon Cc: Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Diamond Knives Have a look at Delaware Diamond Knives (DDK). Their website I believe is www.ddk.com. I'm sure they can help you or at a minimum point you in the right direction. Best Regards, Jack On Mar 14, 2012, at 9:42 PM, Philip Slakmon slak...@yahoo.com wrote: Good Afternoon, I was interested in knowing people's opinions on the different diamond knives on the market. Opinions could be based on quality, design, workmanship, delivery, customer service/support, pricing, Thank you, Philip ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] CD230
Does anyone have any experience with or knowledge of this antibody (CD230)? I would appreciate any comments/literature you could share. Thank you, Kim Kimberly R. Artim AST, HT (ASCP) St Luke's Specialty Lab 77 S. Commerce Way Bethlehem, PA 18017 Phone: 484-526-4832 art...@slhn.orgmailto:art...@slhn.org ** Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] PIN-4
Does anyone have a good protocol for pin-4 on a Ventana XT or Ultra? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Validation of new equipment
If I am validating a new tissue processor, can I just process a v ariety of tissues and have the pathologist sign off that they are acce= ptable, or do I need to do a parallel run with tissue on an established pro= cessor and compare the results? Laurie ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] PAS Diastase
My two cents: I agree as well Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Geoff McAuliffe Sent: Friday, 16 March 2012 12:55 AM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] PAS Diastase I agree with Rene. A control (undigested) slide is necessary. Geoff On 3/15/2012 1:47 AM, Jennifer MacDonald wrote: Hi All, At a local lab when a pathologist orders a PAS diastase the histotechnicians do just one slide with diastase. They do not do an undigested slide. How would the pathologist know if the digested slide had a glycogen to begin with? Am I over thinking this? Thanks, Jennifer ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- -- ** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcaul...@umdnj.edu ** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet * This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. * ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Recall: [Histonet] PAS Diastase
Tony Henwood (SCHN) would like to recall the message, [Histonet] PAS Diastase. * This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. * ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Re: PAS Diastase
No, If you need to demonstrate that PAS positive material is glycogen and not some other carbohydrate containing material you must do a PAS on the test case as well. (see my earlier Post) Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Theresa (Teri) Johnson Sent: Friday, 16 March 2012 3:22 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: PAS Diastase I disagree that two patient slides are needed. What is needed for diagnosis is one patient slide with digestion, and two control slides, one with and one without. If the control slide shows adequate digestion of the glycogen and the other control slide shows adequate PAS positivity, the pathologist can be assured that if the patient slide has any PAS positivity it is not due to glycogen. I am guessing this is only in cases where they are not concerned with glycogen storage as part of the diagnosis (liver biopsy). Ask your pathologist what he or she is looking for. My guess is it is not glycogen, but some other PAS positive component. On 3/15/2012 1:47 AM, Jennifer MacDonald wrote: Hi All, At a local lab when a pathologist orders a PAS diastase the histotechnicians do just one slide with diastase. They do not do an undigested slide. How would the pathologist know if the digested slide had a glycogen to begin with? Am I over thinking this? Thanks, Jennifer Teri Johnson, HT(ASCP)QIHC GNF Histology Lab Manager Genomics Institute of the Novartis Research Foundation 858-332-4752 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet * This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. * ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Staining GAGs in fetal membranes
Hello, I am attempting to stain fetal membranes to look at the GAG content. My advisor suggested Safronin O, which I tried on a preliminary set of slides (hematoxylin, fast green, then safronin O). I did get some staining and saw a band of red staining between the layers, as expected, but the staining is not nearly as pronounced as in images of Safronin O stained tissues that I have seen (some of this obviously may be due to imaging settings). Also, after doing some reading, it seems that Alcian blue may be another option. Can anyone with experience staining FMs or similar tissues suggest a stain and protocol for this? Thanks! Regards, Jennifer Scholler M.S. candidate Mechanical Engineering University of Colorado at Boulder ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Re: PAS Diastase
Tony, I agree, when looking for glycogen two patient slides should be run, one with, one without. That is not always the case. We were sometimes asked to do PAS/D on lymphomas. Teri -Original Message- From: Tony Henwood (SCHN) [mailto:tony.henw...@health.nsw.gov.au] Sent: Thursday, March 15, 2012 3:39 PM To: Theresa (Teri) Johnson; histonet@lists.utsouthwestern.edu Subject: RE: Re: PAS Diastase No, If you need to demonstrate that PAS positive material is glycogen and not some other carbohydrate containing material you must do a PAS on the test case as well. (see my earlier Post) Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Theresa (Teri) Johnson Sent: Friday, 16 March 2012 3:22 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: PAS Diastase I disagree that two patient slides are needed. What is needed for diagnosis is one patient slide with digestion, and two control slides, one with and one without. If the control slide shows adequate digestion of the glycogen and the other control slide shows adequate PAS positivity, the pathologist can be assured that if the patient slide has any PAS positivity it is not due to glycogen. I am guessing this is only in cases where they are not concerned with glycogen storage as part of the diagnosis (liver biopsy). Ask your pathologist what he or she is looking for. My guess is it is not glycogen, but some other PAS positive component. On 3/15/2012 1:47 AM, Jennifer MacDonald wrote: Hi All, At a local lab when a pathologist orders a PAS diastase the histotechnicians do just one slide with diastase. They do not do an undigested slide. How would the pathologist know if the digested slide had a glycogen to begin with? Am I over thinking this? Thanks, Jennifer Teri Johnson, HT(ASCP)QIHC GNF Histology Lab Manager Genomics Institute of the Novartis Research Foundation 858-332-4752 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet * This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. * ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Wet Workshops NSW Australia
Hi all, We are looking at organising wet workshops in Sydney later this year (Wet workshop = hands on lab work). We ran a Histochem workshop last year at our National Meeting (twice) and many seemed happy with it. We are after themes for further workshops eg: Mucin Histochemistry HE Staining Manual Immunohistochemistry Any other ideas? Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) and Linda Prasad MSc, BSc Histopathology Department Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA * This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. * ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
