[Histonet] anti-Cy3 for FFPE
Does anyone know of an antibody for Cy3 that works on FFPE tissue? Many thanks, Beth Beth Villarreal Scientist I Novartis Institutes for BioMedical Research, Inc. 300 Technology Square Cambridge, MA 02139 USA Phone+1 617 8714725 Fax +1 N.A. beth.villarr...@novartis.commailto:beth.villarr...@novartis.com www.novartis.comhttp://www.novartis.com/ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] GMS on toenails
From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Scott, Allison D Sent: Thursday, April 19, 2012 3:23 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] GMS on Toenail Hello to all in histoland. We have a stubborn toenail that keeps coming off when we try to do a GMS stain on the ventana machine. Any suggestions on how to keep the section on the slide during the staining procedure. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital Houston, Texas Hello Allison, we do PAS and GMS on toenails just about every day since we have a nail clinic in our department. First we dip our slides in Stay On solution (SurgiPath) and allow them to dry then we soak the faced nail block in 4% Ammonium Hydroxide for 45-60 minutes before we cut a ribbon and place on slides. We let the slides sit in a 70 degree oven for about 45 minutes, we Deparaffinize, hydrate to DH2O and we stain on the Artisan Special Stain stainer. Works every time. If it keeps falling off you might need to do it manually. Good Luck -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histonet-requ...@lists.utsouthwestern.edu Sent: Saturday, April 21, 2012 1:03 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 101, Issue 28 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than Re: Contents of Histonet digest... Today's Topics: 1. Re: Histonet Digest, Vol 101, Issue 27 (Eric Tambutte) 2. Re: Pinning Specimens (Bob Richmond) 3. Immunofluorescence (Thotakura, Anil Kumar) 4. RE: GMS on Toenail (Britton, Josette C) 5. Leica Peloris Processor (Joe W. Walker, Jr.) 6. Re: Leica Peloris Processor (Richard Cartun) 7. Re: Leica Peloris Processor (Patrick Laurie) 8. Re: Histonet Digest, Vol 101, Issue 27 (Amos Brooks) -- Message: 1 Date: Fri, 20 Apr 2012 19:03:08 +0200 From: Eric Tambutte etambu...@centrescientifique.mc Subject: [Histonet] Re: Histonet Digest, Vol 101, Issue 27 To: histonet@lists.utsouthwestern.edu Message-ID: 1083480...@s15272523.onlinehome-server.info Bonjour, Je suis absent du laboratoire jusqu'au jeudi 03 mai 2012. Je vous répondrai le plus rapidement possible. Eric Tambutté Thank you for your mail. I will be out of office till May 03rd 2012. I will respond to your e-mail as soon as possible. Thank you for your understanding. Best regards Eric Tambutté -- Message: 2 Date: Fri, 20 Apr 2012 13:20:15 -0400 From: Bob Richmond rsrichm...@gmail.com Subject: [Histonet] Re: Pinning Specimens To: histonet@lists.utsouthwestern.edu Message-ID: caoksrh5gcgsm6hesayw40jr0lfxssre_f42ze0hde1pve0e...@mail.gmail.com Content-Type: text/plain; charset=ISO-8859-1 About pinning specimens so they fix flat: Margaret Horne notes the use of dental wax. I've used it to make small boats for the rather exacting procedure of pinning muscle biopsy specimens for electron microscopy. Here a 1 to 2 mm bundle of longitudinal fibers has to be gently stretched to its resting length, pinned in the boat, and promptly fixed in glutaraldehyde. One technologist noted that she had a young pathologist who was wet behind the ears and wanted to pin specimens, and was looking to the older pathologists to bring him in line to The Way We've Always Done It. I try to remain wet behind the ears, though after nearly 50 years of occasionally pinning specimens I find myself a little dry. We continue to lose skills in the gross room. Today I'm working with a service that has almost no ability to handle calcified tissue - I need to go to the hardware store and buy a hacksaw to replace the worn-out Satterlee saw, which I think was gathered up off a Civil War battlefield. Bob Richmond Samurai Pathologist Knoxville TN -- Message: 3 Date: Fri, 20 Apr 2012 18:01:17 + From: Thotakura, Anil Kumar a.thotak...@imperial.ac.uk Subject: [Histonet] Immunofluorescence To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Message-ID: cbb762fa.14aa6%a.thotak...@imperial.ac.uk Content-Type: text/plain; charset=us-ascii Dear All, Can some one please send the protocol for immunofluorescence, I want to stain for FITC CD45.1 on mice spleens. Thank you very much for your help. Many Thanks, Anil kumar. -- Message: 4 Date: Fri, 20 Apr 2012 14:54:29 -0400
[Histonet] Atlanta Based Field IHC Tech - Great Package!!
Good Morning, We are searching for a Field Based IHC Technician needed in Atlanta to join a global leading manufacturer of Histology products. Perfect for someone looking to break out of the lab and into the field!! Contact m...@personifysearch.com Our client is growing at a rapid pace and has a Field Support Specialist opportunity based in Atlanta to serve as the technical expert on IHC. This position offers an extremely competitive package and opportunity for career growth. If you or anyone you may know would be interested please contact me at m...@personifysearch.com or 800.875.6188 ext. 103 to learn more! Regards, Matt Ward *Account Executive* *Personify* 5020 Weston Parkway Suite 315 Cary NC 27513 (Tel) 800.875.6188 direct ext 103 (Fax) 919.460.0642 www.personifysearch.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] GMS on toenails
We soak toenail specimens in 4% ammonia hydroxide the day before (before processing). After embedding, the nails are immediately sectioned and mounted on slides which were previously dipped in 5% gelatin. An HE, PAS and GMS is done on each nail specimen. This procedure speeds up the overall turn-around time and prevents sections from falling off. Jim ___ James E. Staruk HT(ASCP) www.masshistology.com www.nehorselabs.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Santiago, Albert Sent: Monday, April 23, 2012 8:24 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] GMS on toenails From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Scott, Allison D Sent: Thursday, April 19, 2012 3:23 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] GMS on Toenail Hello to all in histoland. We have a stubborn toenail that keeps coming off when we try to do a GMS stain on the ventana machine. Any suggestions on how to keep the section on the slide during the staining procedure. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital Houston, Texas Hello Allison, we do PAS and GMS on toenails just about every day since we have a nail clinic in our department. First we dip our slides in Stay On solution (SurgiPath) and allow them to dry then we soak the faced nail block in 4% Ammonium Hydroxide for 45-60 minutes before we cut a ribbon and place on slides. We let the slides sit in a 70 degree oven for about 45 minutes, we Deparaffinize, hydrate to DH2O and we stain on the Artisan Special Stain stainer. Works every time. If it keeps falling off you might need to do it manually. Good Luck ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
FW: [Histonet] Part Time Job Opening, Springfield, MA
From: aajl7...@hotmail.com To: histonet@lists.utsouthwestern.edu Date: Fri, 20 Apr 2012 09:10:08 -0400 Subject: [Histonet] Part Time Job Opening, Springfield, MA Please Send Resume for: Part Time opportunity for a Histotechnican. Duties and responsibilities include: • Under general supervision performs routine and non-routine activities involved in the preparation of slides, for microscopic evaluation by pathologist(s) according to policies and procedures • Process paperwork associated with accessioning and reporting. • Eligible to gross per CLIA requirements. • Ensure proper tissue processing. • Embed processed tissue in paraffin • Prepare slides for routine staining. • Perform microtomy of embedded tissue • Perform coverslipping of stained slides either manually or automated. • Perform filing of finished blocks and slides. • Perform routine maintenance and cleaning of equipment and troubleshoot minor equipment failures. Document remedial actions such as repairs or repeated tests. • Adhere to laboratory's quality control policies, and document all quality control activities. • Ensure all corporate safety, quality control and quality assurance standards are met. • Ensure compliance with all local, federal, CLIA and CAP regulations. • Maintain a clean and well-organized work area. • Clinical Chemistry other duties, as assigned by supervisor. Minimum Requirements Include: • AA or AS degree or equivalent training and experience , CLIA approved for grossing specimens • HT (ASCP) or HTL (ASCP) eligible OR five years of full time experience in the last 10 years. • Work Experience: 1-3 years experience as a Histotechnician in an anatomic pathology laboratory. • License for Clinical Laboratory Technician or Technologist license. ***Note this is a part time position also with coverage for vacation Thanks! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Who pays ......
for the express shipping when another medical institution requests a patient's pathology slides and/or unstains or paraffin block for additional testing? In my opinion, the requesting institution should provide a FedEx, UPS or other account number for this. After all, they will be billing the patient's insurance for the consult and/or special testing that is to be performed. This is a major cost to our Department that, as you know, cannot be billed to insurance. I have instructed our staff to ask for an account number; many facilities supply one, others do not. What do you do at your facility? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Who pays ......
We ask the requesting facility to pay shipping as well. Usually they comply. Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Monday, April 23, 2012 10:23 AM To: Histonet Subject: [Histonet] Who pays .. for the express shipping when another medical institution requests a patient's pathology slides and/or unstains or paraffin block for additional testing? In my opinion, the requesting institution should provide a FedEx, UPS or other account number for this. After all, they will be billing the patient's insurance for the consult and/or special testing that is to be performed. This is a major cost to our Department that, as you know, cannot be billed to insurance. I have instructed our staff to ask for an account number; many facilities supply one, others do not. What do you do at your facility? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] neutral buffered formalin fixed tissue and the Golgi-Cox stain
Hello, I'm working with very small fish brains and would like to perform the modified Golgi-Cox stain of http://www.ncbi.nlm.nih.gov/pubmed/21228908 . As my area of interest is near the edge of the tissue I need to coat in egg yolk or gelatine to prevent the dark crust which forms on the tissue (is there another way?). Manipulating my tiny brains in this way in a raw unfixed state is likely to cause significant tissue damage. I have found one article where Golgi-Cox was used on neutral buffered formalin (NBF) fixed tissue (the entire pig brain, http://www.ncbi.nlm.nih.gov/pubmed/2459816) but I can't get hold of the article to actually see how this turned out. Has anyone had any experience using the Golgi-Cox stain on NBF tissue? Where there any problems? (I don't want to kill fish for nothing.) On a related matter, anyone find any faults with the protocol/solutions at http://www.funjournal.org/images/stories/downloads/2011_Volume_10_Issue_1/wright_10_1_a85_a87.pdf ? Thanks -- Tyrone Genade http://tgenade.freeshell.org email: tgen...@gmail.com tel: +27-84-632-1925 (c) Romans 6:23: The gift of God is eternal life through Christ Jesus our Lord. To find out how to receive this FREE gift visit http://www.alpha.org. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Barcoding specimen tracking, lessons you learned
To anyone who has implemented a barcoding/specimen tracking system in your lab. What lessons did you learn that would make it easier if you did it over? We're starting the process and I would like to get some input on things to look out for! Thanks for any info and comments! Tim Morken Department of Pathology UC San Francisco Medical Center 505 Parnassus Ave, Box 1656 Room S570 San Francisco, CA 94132 (415) 353-1266 (ph) (415) 514-3403 (fax) tim.mor...@ucsfmedctr.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Glycosamineglycans quantitation
Hi histonetters'! Does anyone know the difference in glycosamineglycans quantification with 1,9-dimethyl-methylene blue or alcian blue (pH 2.5)? Thank you all Silvina ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Barcoding specimen tracking, lessons you learned
See Rich Pucci at UCSF Pathology. He would be a great resource. Sent from my Windows Phone From: Morken, Timothy Sent: 4/23/2012 10:31 AM To: Histonet Subject: [Histonet] Barcoding specimen tracking, lessons you learned To anyone who has implemented a barcoding/specimen tracking system in your lab. What lessons did you learn that would make it easier if you did it over? We're starting the process and I would like to get some input on things to look out for! Thanks for any info and comments! Tim Morken Department of Pathology UC San Francisco Medical Center 505 Parnassus Ave, Box 1656 Room S570 San Francisco, CA 94132 (415) 353-1266 (ph) (415) 514-3403 (fax) tim.mor...@ucsfmedctr.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Qualifications for grossing
All, Can a histotech perform GROSSING if he/she has an associate's degree in Histotechnology from an accredited institution (Argosy in MN)? Any help would be appreciated. Thank-you, Glen Dawson BS, HT(ASCP) QIHC Histology Technical Specialist Mercy Health System Janesville, WI ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Biospecimen collection
This inquiry applies to those of you collecting human tissue for research. I am being asked to develop pricing for the following activities regarding collection of biospecimens from surgery, image-guided biopsies, endoscopic biopsies, bone marrow biopsies, and body fluids: 1. Collection of fresh tissue and freezing in liquid nitrogen. 2. Collection of fresh tissue and placement in tissue culture media. 3. Preparation of one paraffin block containing formalin-fixed tissue. 4. The preparation of one unstained slide from a paraffin block. I would appreciate hearing from those of you who might already have charges established for these activities. Thank you very much for your time. Happy National Medical Laboratory Professionals Week to all! Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Histology Cassettes - Large or Extra Large
Hello all, I am looking for extra large histology cassettes that will hold specimens that are a max. 20mm thick (high). Is there such a thing? Where would I find these larger cassettes? Thank you, Merissa ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Histology Cassettes - Large or Extra Large
Cassettes used to process eyes should do the trick. Fisher Scientific* Tissue Path* MACROSETTE* Processing/Embedding Cassettes Walter Benton HT(ASCP)QIHC Histology Supervisor Chesapeake Urology Associates 806 Landmark Drive, Suite 126 (All Deliveries to Suite 127) Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) wben...@cua.md From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of M.O. [modz9...@gmail.com] Sent: Monday, April 23, 2012 6:40 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Histology Cassettes - Large or Extra Large Hello all, I am looking for extra large histology cassettes that will hold specimens that are a max. 20mm thick (high). Is there such a thing? Where would I find these larger cassettes? Thank you, Merissa ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Qualifications for grossing
Glen, Below are the requirements for high complexity testing, as outline by CLIA. You can reference the CLIA '88 ruling, specifically look at Subpart M, Section 493.1489 The requirements are weak, to say the least. I am not alone in the opinion that just because CLIA allows it, it is not necessarily appropriate for the minimum qualified person to be grossing certain specimens. Having someone other than an M.D., or ASCP certified PA do anything larger than a skin shave is not good medicine. But, in answer to your question - yes, the government allows inadequately trained personnel to perform high complexity testing. Sec. 493.1489 Standard; Testing personnel qualifications. Each individual performing high complexity testing must-- (a) Possess a current license issued by the State in which the laboratory is located, if such licensing is required; and (b) Meet one of the following requirements: (1) Be a doctor of medicine, doctor of osteopathy, or doctor of podiatric medicine licensed to practice medicine, osteopathy, or podiatry in the State in which the laboratory is located or have earned a doctoral, master's or bachelor's degree in a chemical, physical, biological or clinical laboratory science, or medical technology from an accredited institution; (2)(i) Have earned an associate degree in a laboratory science, or medical laboratory technology from an accredited institution or-- (ii) Have education and training equivalent to that specified in paragraph (b)(2)(i) of this section that includes-- (A) At least 60 semester hours, or equivalent, from an accredited institution that, at a minimum, include either-- (1) 24 semester hours of medical laboratory technology courses; or (2) 24 semester hours of science courses that include-- (i) Six semester hours of chemistry; (ii) Six semester hours of biology; and (iii) Twelve semester hours of chemistry, biology, or medical laboratory technology in any combination; and (B) Have laboratory training that includes either of the following: (1) Completion of a clinical laboratory training program approved or accredited by the ABHES, the CAHEA, or other organization approved by HHS. (This training may be included in the 60 semester hours listed in paragraph (b)(2)(ii)(A) of this section.) (2) At least 3 months documented laboratory training in each specialty in which the individual performs high complexity testing. (3) Have previously qualified or could have qualified as a technologist under Sec. 493.1491 on or before February 28, 1992 On Mon, Apr 23, 2012 at 1:19 PM, Glen Dawson ihcman2...@hotmail.com wrote: All, Can a histotech perform GROSSING if he/she has an associate's degree in Histotechnology from an accredited institution (Argosy in MN)? Any help would be appreciated. Thank-you, Glen Dawson BS, HT(ASCP) QIHC Histology Technical Specialist Mercy Health System Janesville, WI ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- *David Costanzo, MHS, PA (ASCP)* Project Manager *Blufrog Path Lab Solutions* 9401 Wilshire Blvd. Ste 650 Beverly Hills, CA 90212 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
