RE: [Histonet] Re: Help! Liver mistakenly processed in paraffin (had to be in OCT instead)!
Perhaps all is not lost as you will be able to see on a H@E the spaces
reluctantly vacated by the lipids.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of z o n k e d
Sent: 13 November 2012 17:44
To: Jennifer MacDonald
Cc: histonet@lists.utsouthwestern.edu; histonet-boun...@lists.utsouthwestern.edu
Subject: [Histonet] Re: Help! Liver mistakenly processed in paraffin (had to be
in OCT instead)!
We just wanted to see general lipids, nothing in particular. This mouse died
unexpectedly and may have been part of a group that was put on a high fat or
high bile acid diet and we just wanted to see what happened.
On Tuesday, November 13, 2012, Jennifer MacDonald wrote:
It depends on what you are using the oil red o for. Lipofuscin and
ceroid can be demonstrated with an oil red o stain after processing.
Jennifer MacDonald
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Date:11/13/2012 08:53 AM
Subject:[Histonet] Help! Liver mistakenly processed in paraffin
(had to bein OCT instead)!
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Hello Histonetters,
First time writer, long time reader. I'm a newbie tech in academia and
I was given a simple task which I think I pretty much screwed up.
I should have embedded half of a mouse liver in paraffin for microtome
sectioning while the other half should have been embedded in OCT for
cryosectioning (for oil red o). I made the mistake last night of
placing both liver halves into the tissue processor. The liver I
intended for OCT embedding is now hard as wax. Is there any way to
deparaffinize processed organs and may I embed them in OCT for proper
cryosectioning? I imagine that the liver would get dehydrated, I would
get crappy sections, and Oil Red O won't work.
Any suggestions are welcome.
Thank you so much,
Zoe W.
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[Histonet] Technovit 9100 new fails to polymerize
Hello, I got the same problem, how did you solve it ? Best regards Jean-Philippe ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Technovit 9100 new fails to polymerize
Hello, As I wrote you some weeks ago, we started to use Technovit 9100 New for tissue embedding. You helped me to solve my previous problems. And I hope, you will help me to solve my present problems. Sorry, I can explain my problem as follow : The main problem is that we are having some troubles with the polymerization of Technovit 9100 New. The polymerization mixture does not want to become hard. We filled trial moulds with 3 ml of polymerization mixture. Moulds were vacuumed and sealed. We tried to perform the polymerization reaction at -30 degrees C, -20 degrees C, -4 degrees C, +4 degrees C, +20 degrees C. Technovit 9100 New fails to polymerize. After a week, the polymerization mixture becomes gelatinous but not hard. We used the manufacturer's protocol. The preparation of the polymerization mixture was precise (I accurately prepared stock solutions several times). I cannot understand where is the problem May be it is necessary to add more activator (dibenzoyl peroxide and N,N-3,5-tetramethylaniline). May be it is necessary to mix stock solutions A and B in another proportion (not 9:1). Is it necessary to prepare the solution at 4 degrees too ? What should I do? Thank you in advance, ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: staffing numbers
Allison, We are right around 7k cases/year, 20k blocks, and about 33k slides. We have 3 tech including myself as cutter and embedder. The other two tech also cover grossing, special stains, IHC, and cytology prep. We have 1 lab assistant who also helps out with grossing (doesn't do frozen) and cytology. We are also looking to hire another assistant to do filing, etc. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.org www.LMHealth.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Hutton, Allison Sent: Tuesday, November 13, 2012 11:00 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] staffing numbers Hi Everyone, I am looking for some crude numbers regarding staffing. I would like to know the number of cases done per year and your number of histotechs. Any information will be appreciated Thank you Allison ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Technovit 9100 new fails to polymerize
We had the same problems. We switched to Immunobed and that is wonderful. Sets up every single time. Give it a try. Best regards, Nanne -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jean-Philippe Berteau Sent: Wednesday, November 14, 2012 4:14 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Technovit 9100 new fails to polymerize Hello, As I wrote you some weeks ago, we started to use Technovit 9100 New for tissue embedding. You helped me to solve my previous problems. And I hope, you will help me to solve my present problems. Sorry, I can explain my problem as follow : The main problem is that we are having some troubles with the polymerization of Technovit 9100 New. The polymerization mixture does not want to become hard. We filled trial moulds with 3 ml of polymerization mixture. Moulds were vacuumed and sealed. We tried to perform the polymerization reaction at -30 degrees C, -20 degrees C, -4 degrees C, +4 degrees C, +20 degrees C. Technovit 9100 New fails to polymerize. After a week, the polymerization mixture becomes gelatinous but not hard. We used the manufacturer's protocol. The preparation of the polymerization mixture was precise (I accurately prepared stock solutions several times). I cannot understand where is the problem May be it is necessary to add more activator (dibenzoyl peroxide and N,N-3,5-tetramethylaniline). May be it is necessary to mix stock solutions A and B in another proportion (not 9:1). Is it necessary to prepare the solution at 4 degrees too ? What should I do? Thank you in advance, ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] TBS Slide Printer
I am posting a question for a friend. She is looking for feedback on the TBS Shurmark slide marking instrument. All comments are appreciated. Thanks. Christie ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: liftin tissue
We use Mount quick media, which comes with a procedure for section transfer Vanessa -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lee Loss Sent: Tuesday, November 13, 2012 3:21 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] liftin tissue I need to lift and transfer one stained section from a slide and transfer it to another slide to destain the HE and run an immuno on it. Does anyone have a procedure for doing that? Thank you. Lee The materials and information in this e-mail are confidential and may contain Protected Health Information covered under the HIPAA Privacy Rule. If you are not the intended recipient, be advised that any unauthorized use, disclosure, copying, distribution, or action taken in reliance on the contents of this information is strictly forbidden by law. If you have received this e-mail in error, please notify me by reply e-mail and then delete this message. Do not pass any of this information to anyone else. Thank you for your cooperation. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Zinc formalin as primary fixative for TEM
Hello everyone, A colleague has tissues which are fixed in zinc formalin and wants to examine them by transmission electron microscopy. Any thoughts regarding the use of this fixative for EM? Thanks in advance, Carla Conway Histology Technician Western Fisheries Research Center, USGS 6505 N.E. 65th Street Seattle, WA 98115-5016 USA Phone: 206-526-6282 ext. 242 Fax: 206-526-6654 E-mail: cmcon...@usgs.gov ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] HE Question
Hi All, I am having a problem with my staining, ( well, I think its pretty good but the path says its not cark enough for her) So, I am using Azer Scientific Hematoxylin Extra and I initially started out with 2.5 min in it. I upped it to 5 min and then to 10 and 12 min. The problem is the control I have works and looks great at the 2.5 min. Besides just keeping it in the heme longer what else can I do? I have cut a few extra dummy slides today so I can try different things out with them. I am tired of hearing from the path everyday that the heme is too light for her. I am a new lab for a derm clinic and I work alone so its basiclly up to me to figure this out. Please help! Thanks! Jackie ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] HE Question
Jaclyn it sounds daft but check the pH of your Eosin we found this can be strongly acidic. Peter Peter L Craven FIBMS Pathology Department Raigmore Hospital From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jaclyn Pitts [pitts.jac...@gmail.com] Sent: 14 November 2012 03:50 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HE Question Hi All, I am having a problem with my staining, ( well, I think its pretty good but the path says its not cark enough for her) So, I am using Azer Scientific Hematoxylin Extra and I initially started out with 2.5 min in it. I upped it to 5 min and then to 10 and 12 min. The problem is the control I have works and looks great at the 2.5 min. Besides just keeping it in the heme longer what else can I do? I have cut a few extra dummy slides today so I can try different things out with them. I am tired of hearing from the path everyday that the heme is too light for her. I am a new lab for a derm clinic and I work alone so its basiclly up to me to figure this out. Please help! Thanks! Jackie ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message may contain confidential information. If you are not the intended recipient please inform the sender that you have received the message in error before deleting it. Please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Thank you for your co-operation. NHSmail is the secure email and directory service available for all NHS staff in England and Scotland NHSmail is approved for exchanging patient data and other sensitive information with NHSmail and GSi recipients NHSmail provides an email address for your career in the NHS and can be accessed anywhere ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] HE Question
Jackie - Try a different hematoxylin - we switched to Platinum Line Modified Harris Hematoxylin - we save money and the pathologists prefer the results. Tresa -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jaclyn Pitts Sent: Wednesday, November 14, 2012 8:51 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HE Question Hi All, I am having a problem with my staining, ( well, I think its pretty good but the path says its not cark enough for her) So, I am using Azer Scientific Hematoxylin Extra and I initially started out with 2.5 min in it. I upped it to 5 min and then to 10 and 12 min. The problem is the control I have works and looks great at the 2.5 min. Besides just keeping it in the heme longer what else can I do? I have cut a few extra dummy slides today so I can try different things out with them. I am tired of hearing from the path everyday that the heme is too light for her. I am a new lab for a derm clinic and I work alone so its basiclly up to me to figure this out. Please help! Thanks! Jackie ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Histology Supervisor Needed in Atlanta. Can you help?
Hi Histonetters! I hope everybody is having a great day. I wanted to drop you a quick line to ask for help because I am currently recruiting for one of my best clients located in Atlanta, GA. We are looking for an experienced Histology Supervisor. We are looking for someone who is ASCP certified with at least 3 years of histology and 2 years of experience supervising a histology lab. They are offering a great salary, terrific benefits, the stability of a large company and a great group of people to work with. The help I need from you is do you know anyone that might be interested in hearing about this opportunity? If so could you please forward my e-mail to them? If you are interested in this position please contact me ASAP at rel...@earthlink.net or toll free at 866-607-3542. Remember if I hire someone you refer you will receive a referral bonus! Thank you, Pam - 866-607-3542 (866-60RELIA) - Toll Free Thank You! Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: rel...@earthlink.net www.facebook.com http://www.facebook.com/PamBarkerRELIA /PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] HE Question
I never heard of Azer Scientific hematoxylin but if you can leave the sections in it during 12 minutes and it is still weak for your pathologist, it seems that it is a progressive hematoxylin, of the Mayer type. Progressive hematoxylins are somewhat tricky and have to be very fresh to work well. I have always used and preferred regressive hematoxylins of the Harris' type. With them you are in control of the staining when you differentiate them up to the strength proffered by your pathologist. My advise, since it seems that you cannot satisfy your pathologist's demands,is to switch to a Harris type hematoxylin and stop fiddling with the hematoxylin you are using. I always used and preferred Harris by Richard Alan. Switch and your problems will be over. René J. From: Jaclyn Pitts pitts.jac...@gmail.com To: histonet@lists.utsouthwestern.edu Sent: Wednesday, November 14, 2012 10:50 AM Subject: [Histonet] HE Question Hi All, I am having a problem with my staining, ( well, I think its pretty good but the path says its not cark enough for her) So, I am using Azer Scientific Hematoxylin Extra and I initially started out with 2.5 min in it. I upped it to 5 min and then to 10 and 12 min. The problem is the control I have works and looks great at the 2.5 min. Besides just keeping it in the heme longer what else can I do? I have cut a few extra dummy slides today so I can try different things out with them. I am tired of hearing from the path everyday that the heme is too light for her. I am a new lab for a derm clinic and I work alone so its basiclly up to me to figure this out. Please help! Thanks! Jackie ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] HE Question
I know this is out of the box, but it happened to me so I thought I'd pass this along. We had the same issue with our chief pathologist...hematoxylin was too light. We tried everything, but she still complained!! Then we diluted our Eosin Y 1:1 with 95% alcohol and then she was happy. Crazy, I know, but something to think about! Best of luck, and please let us know your findings! Lynette From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of Goins, Tresa [tgo...@mt.gov] Sent: Wednesday, November 14, 2012 11:02 AM To: Jaclyn Pitts; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] HE Question Jackie - Try a different hematoxylin - we switched to Platinum Line Modified Harris Hematoxylin - we save money and the pathologists prefer the results. Tresa -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jaclyn Pitts Sent: Wednesday, November 14, 2012 8:51 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HE Question Hi All, I am having a problem with my staining, ( well, I think its pretty good but the path says its not cark enough for her) So, I am using Azer Scientific Hematoxylin Extra and I initially started out with 2.5 min in it. I upped it to 5 min and then to 10 and 12 min. The problem is the control I have works and looks great at the 2.5 min. Besides just keeping it in the heme longer what else can I do? I have cut a few extra dummy slides today so I can try different things out with them. I am tired of hearing from the path everyday that the heme is too light for her. I am a new lab for a derm clinic and I work alone so its basiclly up to me to figure this out. Please help! Thanks! Jackie ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] HE Question
I second this one! Been there! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lynette Pavelich Sent: Wednesday, November 14, 2012 12:35 PM To: Goins, Tresa; Jaclyn Pitts; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] HE Question I know this is out of the box, but it happened to me so I thought I'd pass this along. We had the same issue with our chief pathologist...hematoxylin was too light. We tried everything, but she still complained!! Then we diluted our Eosin Y 1:1 with 95% alcohol and then she was happy. Crazy, I know, but something to think about! Best of luck, and please let us know your findings! Lynette From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of Goins, Tresa [tgo...@mt.gov] Sent: Wednesday, November 14, 2012 11:02 AM To: Jaclyn Pitts; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] HE Question Jackie - Try a different hematoxylin - we switched to Platinum Line Modified Harris Hematoxylin - we save money and the pathologists prefer the results. Tresa -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jaclyn Pitts Sent: Wednesday, November 14, 2012 8:51 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HE Question Hi All, I am having a problem with my staining, ( well, I think its pretty good but the path says its not cark enough for her) So, I am using Azer Scientific Hematoxylin Extra and I initially started out with 2.5 min in it. I upped it to 5 min and then to 10 and 12 min. The problem is the control I have works and looks great at the 2.5 min. Besides just keeping it in the heme longer what else can I do? I have cut a few extra dummy slides today so I can try different things out with them. I am tired of hearing from the path everyday that the heme is too light for her. I am a new lab for a derm clinic and I work alone so its basiclly up to me to figure this out. Please help! Thanks! Jackie ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Staffing
We processed about 30,000 blocks in 2011 with 2 FTE Histotechs and 1 PRN tech. I also work the bench about 70% of the time, and we have a FT tech assistant. FYI, there was a study by Kohl et al: The CAPNSH Workload Study published in the Archives of Pathology, Vol 135, June 2011 that addresses this issue. Diana Goodwin Histology Supervisor RWJUHH 609-631-6996 dgood...@rwjuhh.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histonet-requ...@lists.utsouthwestern.edu Sent: Wednesday, November 14, 2012 11:27 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 108, Issue 18 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than Re: Contents of Histonet digest... Today's Topics: 1. On call (Martin, Gary) 2. RE: staffing numbers (Lynette Pavelich) 3. Re: Help! Liver mistakenly processed in paraffin (had to be in OCT instead)! (Jennifer MacDonald) 4. Re: Help! Liver mistakenly processed in paraffin (had to be in OCT instead)! (z o n k e d) 5. Re: staffing numbers (Rene J Buesa) 6. Re: Help! Liver mistakenly processed in paraffin (had to be in OCT instead)! (Rene J Buesa) 7. liftin tissue (Lee Loss) 8. Problem with cardiomyocytes staining (Amos Brooks) 9. Cassette and slide labelling systems (Sheila Adey) 10. RE: Re: Help! Liver mistakenly processed in paraffin (had to be in OCT instead)! (Edwards, Richard E.) 11. Technovit 9100 new fails to polymerize (Jean-Philippe Berteau) 12. Technovit 9100 new fails to polymerize (Jean-Philippe Berteau) 13. RE: staffing numbers (Tom McNemar) 14. RE: Technovit 9100 new fails to polymerize (Marsh, Nannette) 15. TBS Slide Printer (CHRISTIE GOWAN) 16. RE: liftin tissue (Vanessa Perez) 17. Zinc formalin as primary fixative for TEM (Carla M Conway) 18. HE Question (Jaclyn Pitts) 19. RE: HE Question (Craven Peter (NHS HIGHLAND)) 20. RE: HE Question (Goins, Tresa) 21. Histology Supervisor Needed in Atlanta. Can you help? (Pam Barker) 22. Re: HE Question (Rene J Buesa) -- Message: 1 Date: Tue, 13 Nov 2012 10:07:36 -0800 From: Martin, Gary gmar...@marshallmedical.org Subject: [Histonet] On call To: histonet@lists.utsouthwestern.edu Message-ID: 6ED9D4252F278841A0593D3D788AF24C179597D6@mailsvr.MARSHMED.local Content-Type: text/plain; charset=us-ascii This question may be a bit off subject Histonet, but I have not been able to find information on this subject. Our facility has decided to pay our Pathologist for their on call duties. I have been tasked with finding what other facilities are paying for this service. Any information would be appreciated. Thank You Gary -- Message: 2 Date: Tue, 13 Nov 2012 18:54:05 + From: Lynette Pavelich lpave...@hurleymc.com Subject: [Histonet] RE: staffing numbers To: Hutton, Allison ahut...@dh.org, histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Message-ID: 89f4666a496dc949a819ecc40e11c867bf569...@exchangemb1.hmc.hurleymc.com Content-Type: text/plain; charset=us-ascii We process ~14.0K cases/yr and 34.5K blocks/yr. We have 4 techs with me working part time on the bench. Lynette Lynette Pavelich, HT(ASCP) Histology Supervisor Hurley Medical Center One Hurley Plaza Flint, MI 48503 ph: 810.262.9948 mobile: 810.444.7966 From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of Hutton, Allison [ahut...@dh.org] Sent: Tuesday, November 13, 2012 11:00 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] staffing numbers Hi Everyone, I am looking for some crude numbers regarding staffing. I would like to know the number of cases done per year and your number of histotechs. Any information will be appreciated Thank you Allison ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Message: 3 Date: Tue, 13 Nov 2012 11:01:14 -0800 From: Jennifer MacDonald jmacdon...@mtsac.edu Subject: [Histonet] Re: Help! Liver mistakenly processed in paraffin (had to be in OCT instead)! To: z o n k e d zon...@gmail.com Cc: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu,
[Histonet] RE: HE staining
Jackie: If you are using tap water to rinse, check the pH. Acidic tap water can lighten the Hematoxylin staining. Diana Goodwin Histology Supervisor RWJUHH 609-631-6996 dgood...@rwjuhh.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histonet-requ...@lists.utsouthwestern.edu Sent: Wednesday, November 14, 2012 11:27 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 108, Issue 18 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than Re: Contents of Histonet digest... Today's Topics: 1. On call (Martin, Gary) 2. RE: staffing numbers (Lynette Pavelich) 3. Re: Help! Liver mistakenly processed in paraffin (had to be in OCT instead)! (Jennifer MacDonald) 4. Re: Help! Liver mistakenly processed in paraffin (had to be in OCT instead)! (z o n k e d) 5. Re: staffing numbers (Rene J Buesa) 6. Re: Help! Liver mistakenly processed in paraffin (had to be in OCT instead)! (Rene J Buesa) 7. liftin tissue (Lee Loss) 8. Problem with cardiomyocytes staining (Amos Brooks) 9. Cassette and slide labelling systems (Sheila Adey) 10. RE: Re: Help! Liver mistakenly processed in paraffin (had to be in OCT instead)! (Edwards, Richard E.) 11. Technovit 9100 new fails to polymerize (Jean-Philippe Berteau) 12. Technovit 9100 new fails to polymerize (Jean-Philippe Berteau) 13. RE: staffing numbers (Tom McNemar) 14. RE: Technovit 9100 new fails to polymerize (Marsh, Nannette) 15. TBS Slide Printer (CHRISTIE GOWAN) 16. RE: liftin tissue (Vanessa Perez) 17. Zinc formalin as primary fixative for TEM (Carla M Conway) 18. HE Question (Jaclyn Pitts) 19. RE: HE Question (Craven Peter (NHS HIGHLAND)) 20. RE: HE Question (Goins, Tresa) 21. Histology Supervisor Needed in Atlanta. Can you help? (Pam Barker) 22. Re: HE Question (Rene J Buesa) -- Message: 1 Date: Tue, 13 Nov 2012 10:07:36 -0800 From: Martin, Gary gmar...@marshallmedical.org Subject: [Histonet] On call To: histonet@lists.utsouthwestern.edu Message-ID: 6ED9D4252F278841A0593D3D788AF24C179597D6@mailsvr.MARSHMED.local Content-Type: text/plain; charset=us-ascii This question may be a bit off subject Histonet, but I have not been able to find information on this subject. Our facility has decided to pay our Pathologist for their on call duties. I have been tasked with finding what other facilities are paying for this service. Any information would be appreciated. Thank You Gary -- Message: 2 Date: Tue, 13 Nov 2012 18:54:05 + From: Lynette Pavelich lpave...@hurleymc.com Subject: [Histonet] RE: staffing numbers To: Hutton, Allison ahut...@dh.org, histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Message-ID: 89f4666a496dc949a819ecc40e11c867bf569...@exchangemb1.hmc.hurleymc.com Content-Type: text/plain; charset=us-ascii We process ~14.0K cases/yr and 34.5K blocks/yr. We have 4 techs with me working part time on the bench. Lynette Lynette Pavelich, HT(ASCP) Histology Supervisor Hurley Medical Center One Hurley Plaza Flint, MI 48503 ph: 810.262.9948 mobile: 810.444.7966 From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of Hutton, Allison [ahut...@dh.org] Sent: Tuesday, November 13, 2012 11:00 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] staffing numbers Hi Everyone, I am looking for some crude numbers regarding staffing. I would like to know the number of cases done per year and your number of histotechs. Any information will be appreciated Thank you Allison ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Message: 3 Date: Tue, 13 Nov 2012 11:01:14 -0800 From: Jennifer MacDonald jmacdon...@mtsac.edu Subject: [Histonet] Re: Help! Liver mistakenly processed in paraffin (had to be in OCT instead)! To: z o n k e d zon...@gmail.com Cc: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu, histonet-boun...@lists.utsouthwestern.edu histonet-boun...@lists.utsouthwestern.edu Message-ID: of088521f9.9dedeb08-on88257ab5.00687433-88257ab5.00687...@mtsac.edu Content-Type: text/plain;
[Histonet] SPIDIA Prostate Tumor Ring Trial
Hi Histonetters, I need some prostate experts to evaluate some virtual slides for a world wide morphology ring trial. We started the ring trial already in August, but a few of the participants don't respond. If you know anyone who would be interested, please ask them to contact me (m.ka...@erasmusmc.nl) for more info. Thanx! Marcel ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] FW: holes in tissue sections
From: Santiago, Albert Sent: Wednesday, November 14, 2012 1:35 PM To: 'histonet@lists.utsouthwestern.edu' Subject: holes in tissue sections Hello fellow histonetters, We are a derm lab and we are having slide quality issues, I am hoping someone can provide me with some advice on how to resolve this problem: We are experiencing holes in tissue sections. The holes appear mainly in the epidermis but sometimes in the dermis. I've tried a few different things but still haven't resolved. If someone has experienced or seen this please help. Any and all feedback will be appreciated. I have some sample photos for your review, if anyone would like to see what I'm talking about. Please let me know and I'll email them to you. Thank you, Albert The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message.___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: holes in tissue sections
Hi Albert, The first thing that comes to mind to me is aggressive facing or rough cutting of the block before taking your final good section. To solve this, after facing the block, (usually at 20+ microns), I go down to my desired slide micron (4) and finish facing at the lower micron for 5-10 more times. This will remove the holes caused by the high microns. Hope this helps, Lynette From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of Santiago, Albert [albert.santi...@uphs.upenn.edu] Sent: Wednesday, November 14, 2012 1:47 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] FW: holes in tissue sections From: Santiago, Albert Sent: Wednesday, November 14, 2012 1:35 PM To: 'histonet@lists.utsouthwestern.edu' Subject: holes in tissue sections Hello fellow histonetters, We are a derm lab and we are having slide quality issues, I am hoping someone can provide me with some advice on how to resolve this problem: We are experiencing holes in tissue sections. The holes appear mainly in the epidermis but sometimes in the dermis. I've tried a few different things but still haven't resolved. If someone has experienced or seen this please help. Any and all feedback will be appreciated. I have some sample photos for your review, if anyone would like to see what I'm talking about. Please let me know and I'll email them to you. Thank you, Albert The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message.___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] preserving hydrogel in tissue sections
We were asked to perform histology on goat knee joints (fixed in 10%NBF) to demonstrate a polymeric material - hydrogel. Could someone please share their procedure or direct me to someone who could help me? I couldn't find much information using PEG and are open to using other methods. We would like to preserve the hydrogel for microscopic evaluation if at all possible. Thanks in advance. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Gomori Trichrome and nemaline bodies?
Hello All, I work in a core facility providing laboratory services to researchers. I had a request to cut some frozen sections on muscle tissue, and stain them with Gomori Trichrome, a technique we use routinely. I did the stain, checked them microscopically andconfirmed that the collagen fibers were nicely stained. But the researcher came back and said that the slides did not show what she was looking for, which was nemaline bodies, rod-shaped structures indicative of a disease called nemaline myopathy. I had never heard of this but went online and found several articles on the disease, a couple of which showed images of nemaline bodies stained with a modified Gomori Trichrome procedure. But no indications of what the modifications were. Is anyone familiar with the staining of these bodies, either using a modified Gomori trichrome, or some other method? Thanks. Paul Monfils ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Gomori Trichrome and nemaline bodies?
I have attached our Modified Gomori's Trichrome procedure. Lynette From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of Monfils, Paul [pmonf...@lifespan.org] Sent: Wednesday, November 14, 2012 2:40 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Gomori Trichrome and nemaline bodies? Hello All, I work in a core facility providing laboratory services to researchers. I had a request to cut some frozen sections on muscle tissue, and stain them with Gomori Trichrome, a technique we use routinely. I did the stain, checked them microscopically andconfirmed that the collagen fibers were nicely stained. But the researcher came back and said that the slides did not show what she was looking for, which was nemaline bodies, rod-shaped structures indicative of a disease called nemaline myopathy. I had never heard of this but went online and found several articles on the disease, a couple of which showed images of nemaline bodies stained with a modified Gomori Trichrome procedure. But no indications of what the modifications were. Is anyone familiar with the staining of these bodies, either using a modified Gomori trichrome, or some other method? Thanks. Paul Monfils ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] FW: holes in tissue sections
From: joellewea...@hotmail.com To: albert.santi...@uphs.upenn.edu Subject: RE: [Histonet] FW: holes in tissue sections Date: Wed, 14 Nov 2012 19:47:46 + Thank you for the pictures. Yes, this appears to be a physical tearing. I agree with the other postee on the histonet that this is more likely due to microtomy technique. Specifically, due to rough facing, and then taking a section too early after returing to normal section thickness with an automated microtome or too rapid sectioning ( stroke speed)on a manual for the ribbon from which the final section is selected. With some skins, if they are somewhat dense, and (the tissue can sometimes even appear white in color if there are dehydration issues), I find that if you face a little more gingerly and then soak them in some very cold ice water and not just hardened ice, then enough moisture transfers to help make it pliable. I sometimes use the warm water bath and then transfer them back to the cold to get a bit more moisture. This always seems to work pretty well and produces a smoother section without tearing. Most times you can see this artifact on the waterbath if you are watching for it. I never take the first ribbons or sections that appear first on the knife blade with any sections due to this artifact and also the greater thickness of these sections. Alternatively, tearing can occur when the knife blade is not shifted after facing and debris of paraffin and excess tissue dull and remain on the blade( or behind the blade) from facing and then cause tears in sections from the extra drag on the blade edge. Just use one part of the blade only for facing and move to a fresh blade section only for final sections and watch carefully for dragging and tearing as the knift dulls on the water bath. Maybe give these technique adjustments a try first, and I hope that it will simply and quickly solve your issues. Joelle Weaver MAOM, HTL (ASCP) QIHC Subject: RE: [Histonet] FW: holes in tissue sections Date: Wed, 14 Nov 2012 14:28:17 -0500 From: albert.santi...@uphs.upenn.edu To: joellewea...@hotmail.com Hi Joelle, I have attached the photos per your request for your review. Thank you very much for taking the time to look into this. Thank you From: joelle weaver [mailto:joellewea...@hotmail.com] Sent: Wednesday, November 14, 2012 2:04 PM To: Santiago, Albert Subject: RE: [Histonet] FW: holes in tissue sections Yes images would be helpful. Thanks Joelle Weaver MAOM, HTL (ASCP) QIHC Date: Wed, 14 Nov 2012 13:47:36 -0500 From: albert.santi...@uphs.upenn.edu To: histonet@lists.utsouthwestern.edu Subject: [Histonet] FW: holes in tissue sections From: Santiago, Albert Sent: Wednesday, November 14, 2012 1:35 PM To: 'histonet@lists.utsouthwestern.edu' Subject: holes in tissue sections Hello fellow histonetters, We are a derm lab and we are having slide quality issues, I am hoping someone can provide me with some advice on how to resolve this problem: We are experiencing holes in tissue sections. The holes appear mainly in the epidermis but sometimes in the dermis. I've tried a few different things but still haven't resolved. If someone has experienced or seen this please help. Any and all feedback will be appreciated. I have some sample photos for your review, if anyone would like to see what I'm talking about. Please let me know and I'll email them to you. Thank you, Albert The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message.___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message.
[Histonet] test
test ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Control Block Log Sheets
We write the accession number on the control block itself and transfer that ID to the slide label and to the data entry system when the control block is used. This creates a permanent record while eliminating the need for a log that continually needs updating. Tresa -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Fawn Bomar Sent: Wednesday, November 14, 2012 12:23 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Control Block Log Sheets Does anyone have a template they would be willing to share on how to keep track of control blocks? Do you all use a type of log sheet to keep track, do you all number them a certain way to keep track? Thank you Fawn ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
