Re: [Histonet] Fixation
Hi, you can find a pratical suggestion at your problem by watching the Step 9: Expedite Large Specimen Fixation on the guide: 101 Steps to Better Histology - a Practical Guide to Good Histology Practice You can download it from: http://www.leicabiosystems.com/pathologyleaders/101-steps-to-better-histology-a-practical-guide-to-good-histology-practice/ I don't know if such procedure is suitable for the study of your specimen but take it just like a suggestion. My Best. Massimo Tosi A humble Chemical Engineer who loves Histology Da: Giulia Zunino giuli.zun...@gmail.com A: histonet@lists.utsouthwestern.edu Inviato: Mercoledì 13 Marzo 2013 11:31 Oggetto: [Histonet] Fixation I would like to have some informations about fetal brain fixation.. In general, it is in use the fomalin, but during the cut it is possible to see that in the deep layer the formalin doesn't arrive... So, Could you give some suggestion to improve this technique?! Thanks in advance Best Giulia Zunino, PhD Student Laboratory of Molecular Neuropathology Centre for Integrative Biology (CIBIO), University of Trento ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] SOS EGFRV3
Does anyone perform this particular clone of EGFR?? Thanks in advance for your help. Billie Zimmerman MT(ASCP)QIHC 706-721-5617/3630 Augusta State University and Georgia Health Sciences University have consolidated to become Georgia Regents University. Effective January 9, 2013, my email address has changed to bzimm...@gru.edu. Please update your address book to reflect this change. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Technical Laboratory Coordinator of Pathology Surgical Services- Atlanta, GA
Full time/permanent opening for a Lab Coordinator of Pathology Surgical Services. Ideal candidate is HT/HTL/CT certified with at least 4 years of supervisory experience. Send an email to bran...@alliedsearchpartners.com for a full job description. To view a complete list of Allied Search Partners current openings go to: http://www.alliedsearchpartners.com/careers.php -- Brannon Owens Recruitment Manager Allied Search Partners ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Histonet Digest, Vol 112, Issue 13
Have a question to ask the group. One of the Pathologists is complaining about anucleate squames on her breast biopsies. Blames the tech for fingers being in the water bath, which may or may not happen. If she recuts the case it is great and she does not do anything different. what could cause this phenomenon? molds? It is driving us crazy. HELP NOTICE TO RECIPIENT: If you are not the intended recipient of this e-mail, you are prohibited from sharing, copying, or otherwise using or disclosing its contents. If you have received this e-mail in error, please notify the sender immediately by reply e-mail and permanently delete this e-mail and any attachments without reading, forwarding or saving them. Thank you. From: histonet-requ...@lists.utsouthwestern.edu To: histonet@lists.utsouthwestern.edu Date: 03/12/2013 10:03 AM Subject:Histonet Digest, Vol 112, Issue 13 Sent by:histonet-boun...@lists.utsouthwestern.edu Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than Re: Contents of Histonet digest... Today's Topics: 1. Re: Fw: news (Kim Donadio) 2. Detecting monoclonas in mouse lung (Leyva-Grado, Victor) 3. Reagent containers for VIP 3000 (Vickroy, Jim) -- Message: 1 Date: Mon, 11 Mar 2013 13:47:55 -0400 From: Kim Donadio one_angel_sec...@yahoo.com Subject: Re: [Histonet] Fw: news To: Kim Donadio one_angel_sec...@yahoo.com Cc: histonet histonet@lists.utsouthwestern.edu,confirm unsub 2m2s2bpqnls4gcuu00bqawlkaga4rn0q confirm-unsub-2m2s2bpqnls4gcuu00bqawlkaga4r...@yahoogroups.com, Pathrm35 pathr...@comcast.net, PensacolaToyBreedAdoption unsubscribe pensacolatoybreedadoption-unsubscr...@yahoogroups.com Message-ID: faa7e81b-723f-4446-b4a3-e945ca593...@yahoo.com Content-Type: text/plain;charset=us-ascii Disregard something is up with my account. Sent from my iPhone On Mar 11, 2013, at 11:38 AM, Kim Donadio one_angel_sec...@yahoo.com wrote: http://www.britvoice.co.uk/wwvc/woszquloax/hkjmnqu=bvdzq ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Message: 2 Date: Mon, 11 Mar 2013 19:37:29 + From: Leyva-Grado, Victor victor.leyva-gr...@mssm.edu Subject: [Histonet] Detecting monoclonas in mouse lung To: 'histonet@lists.utsouthwestern.edu' histonet@lists.utsouthwestern.edu Message-ID: a7d5472945c4e4448fb56d16f814743d071...@exch-2kx-mbxeb2.exchmail.mssm.edu Content-Type: text/plain; charset=us-ascii Dear all, I have a couple of monoclonal antibodies raised against a respiratory virus. I used as treatment for disease and they worked. In trying to elucidate some mechanisms, I'm planning to biotinylate the antibody and at specific time points collect the lungs and do IHC to determine the distribution of the monoclonal. Do you think this will be feasible? I'm also planning to use some section for double labeling IF to determine the site of interaction antigen-antibody. Do you guys have any reference for this? Thanks a lot, Victor Victor H Leyva-Grado DVM, PhD Postdoctoral Fellow Microbiology Department Global Health and Emerging Pathogens Institute Icahn School of Medicine at Mount Sinai One Gustave L Levy Place Box 1124 Annenberg 16-15 New York, NY 10029 Phone 1-212-241-7094 Fax 1-212-534-1684 -- Message: 3 Date: Tue, 12 Mar 2013 08:11:09 -0500 From: Vickroy, Jim vickroy@mhsil.com Subject: [Histonet] Reagent containers for VIP 3000 To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Message-ID: bb0b9f1a8373f14fa2974e8cb24bf9cf255c2...@mmc-mail.ad.mhsil.com Content-Type: text/plain; charset=us-ascii We have two old VIP 3000's and are trying to keep them running. Unfortunately finding some parts are getting g very hard. Can anyone steer me to who might have some old reagent containers for the VIP 3000? We have one container that started leaking yesterday and I suspect this is going to be an ongoing problem because of the age of the instrument. Thanks for your assistance. Jim James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 This message (including any attachments) contains
[Histonet] Thawing brain tissue
Hello Histonet community, I recently froze a primate brain using isopentane immerse in a bath of 100% ethanol and chunks of dry ice. Two out of the 3 blocks froze fine but the third one shows a very bad convex surface, although to be fair, the defective block was embedded in gelatin while the other ones weren't. We have had this issue before, even without the embedding, and my PI thinks it could be due in part to a bad blocking procedure in which the surface is not completely flat. Before we were able to deal with the convex defect. This time it is so bad that we think it should be better to thaw and re-freeze. When dealing with thawing is usually for other purposes (i.e., live cells retrieval) and I think it is suggested to thaw very fast, to avoid water crystal issues. But in the case of morphology and IHC, will it be beneficial to thaw quickly? I am trying to learn from others experience so I can try so salvage this sample so any information is appreciated. I know this is far from being a standard procedure but since the block got so deformed I don't think there is any other option, I am just trying to find the optimum conditions for a non-ideal situation. Thanks Maria Navas ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Thawing brain tissue
Hello Histonet community, I recently froze a primate brain using isopentane immerse in a bath of 100% ethanol and chunks of dry ice. Two out of the 3 blocks froze fine but the third one shows a very bad convex surface, although to be fair, the defective block was embedded in gelatin while the other ones weren't. We have had this issue before, even without the embedding, and my PI thinks it could be due in part to a bad blocking procedure in which the surface is not completely flat. Before we were able to deal with the convex defect. This time it is so bad that we think it should be better to thaw and re-freeze. When dealing with thawing is usually for other purposes (i.e., live cells retrieval) and I think it is suggested to thaw very fast, to avoid water crystal issues. But in the case of morphology and IHC, will it be beneficial to thaw quickly? I am trying to learn from others experience so I can try so salvage this sample so any information is appreciated. I know this is far from being a standard procedure but since the block got so deformed I don't think there is any other option, I am just trying to find the optimum conditions for a non-ideal situation. Thanks, Maria ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] RE: AFB and negative control
This practice is listed as a QC measure for issues of cross contamination in the ASCP publication Quality Management in Anatomic Pathology, Nakhleh, R. M.D. I have never had any issues that were persistant enough to warrant this measure myself, but it is one of the suggestions made under the section for use of control tissue/slides. Joelle Weaver MAOM, HTL (ASCP) QIHC From: tnma...@mdanderson.org To: histonet@lists.utsouthwestern.edu Date: Thu, 14 Mar 2013 17:52:39 + Subject: [Histonet] RE: AFB and negative control While I don't use a negative control for the AFB, I will use distilled water throughout the procedure. Most of the time the water in the waterbath is distilled as well, to rule out contamination there as well. Make sure the waterbath has been disinfected. Toysha N. Mayer, MBA, HT (ASCP) Instructor, Education Coordinator Program in Histotechnology School of Health Professions MD Anderson Cancer Center (713) 563-3481 tnma...@mdanderson.org Date: Thu, 14 Mar 2013 12:42:30 + From: Ian R Bernard ibern...@uab.edu Subject: [Histonet] AFB and Negative Control To: Lee Peggy Wenk lpw...@sbcglobal.net, Tighe, Sean T sti...@ufl.edu, histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Message-ID: d4f4c602b10b9f45b4e9271af6380e16181a1...@uabexmb1.ad.uab.edu Content-Type: text/plain; charset=utf-8 The only special stain that I know that requires the use of a negative control is for the AFB. I understand to rule out false positives as the AFB bacteria might exist in tap water. Nevertheless, a good QA practice which we will implement now. Other than Carson, does anyone know of a regulatory or accreditation agency is requiring this as well? Any suggestion on a good control tissue type? Carson recommends uterus. Also if there is a pick up on the negative slide (link to the tap water) will use of distilled water and a repeat procedure fix this? Any thoughts from fellow histonetters? Thanks Ian Bernard ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] RE: AFB and negative control
Isn't a gram stain the only special that requires a positive and negative control? Sent from my iPhone On Mar 14, 2013, at 1:51 PM, joelle weaver joellewea...@hotmail.com wrote: This practice is listed as a QC measure for issues of cross contamination in the ASCP publication Quality Management in Anatomic Pathology, Nakhleh, R. M.D. I have never had any issues that were persistant enough to warrant this measure myself, but it is one of the suggestions made under the section for use of control tissue/slides. Joelle Weaver MAOM, HTL (ASCP) QIHC From: tnma...@mdanderson.org To: histonet@lists.utsouthwestern.edu Date: Thu, 14 Mar 2013 17:52:39 + Subject: [Histonet] RE: AFB and negative control While I don't use a negative control for the AFB, I will use distilled water throughout the procedure. Most of the time the water in the waterbath is distilled as well, to rule out contamination there as well. Make sure the waterbath has been disinfected. Toysha N. Mayer, MBA, HT (ASCP) Instructor, Education Coordinator Program in Histotechnology School of Health Professions MD Anderson Cancer Center (713) 563-3481 tnma...@mdanderson.org Date: Thu, 14 Mar 2013 12:42:30 + From: Ian R Bernard ibern...@uab.edu Subject: [Histonet] AFB and Negative Control To: Lee Peggy Wenk lpw...@sbcglobal.net, Tighe, Sean T sti...@ufl.edu,histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Message-ID: d4f4c602b10b9f45b4e9271af6380e16181a1...@uabexmb1.ad.uab.edu Content-Type: text/plain; charset=utf-8 The only special stain that I know that requires the use of a negative control is for the AFB. I understand to rule out false positives as the AFB bacteria might exist in tap water. Nevertheless, a good QA practice which we will implement now. Other than Carson, does anyone know of a regulatory or accreditation agency is requiring this as well? Any suggestion on a good control tissue type? Carson recommends uterus. Also if there is a pick up on the negative slide (link to the tap water) will use of distilled water and a repeat procedure fix this? Any thoughts from fellow histonetters? Thanks Ian Bernard ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
