[Histonet] HT HistoDeck question...
Hi, Please clarify why this answer to the HistoDeck study question is a) and not b). Here is the question: 'Frozen section slides cut from fresh, unfixed tissue specimens are optimally fixed in which of the following solutions? a) 37%-40% formaldehyde b) Cold acetone c) Acetic acid alcohol d) Alcoholic formalin Thanks, Sheryl Stephenson | Histology Technician ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Inconsistent Sections with Cryostat
I believe you may need to have the unit serviced. It sounds like something is not tight enough, perhaps the stage or blade holder unit. You said you secured everything which makes me think you have some issue with the cryostat itself. If a sharp blade, tightened blade and specimen, and varying the speed doesn't work, then you may need professional assistance form a service technician, especially if it is an older unit that has been moved around. Have you tried different temperatures? I don't think that is the problem but it is worth a try. Good luck! Cryosectioning can be an art in itself. Those decent sections are likely thicker than you intend, by the way. Classic thick and thin. Philip Manfre, B.A., HT (ASCP) Associate Principal Scientist Merck Research Laboratories WP45-251 PO Box 4 West Point, PA 19486 215-652-9750 215-993-0383 (fax) philip_man...@merck.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Perow, Elliott S (PEROWES10) Sent: Thursday, October 03, 2013 12:33 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Inconsistent Sections with Cryostat Hello All, I have a Leica Cryocut 1800 with a Reichert-Jung 2020 microtome that has sat for 8 years and went through a move from one academic building to another. I am trying to section brook trout brain, but am having difficulty getting consistent sections. I will get a good section and then the next section will be a very very thin shaving of usually just the top portion of the OCT block. I have tried adjusting the clearance angle, made sure the blade and specimen is secured, replaced the blade with a new one, have tried different sectioning speeds, different thicknesses of the section and still continually only get a decent section every other turn. I was wondering if anyone has had any similar experiences or if maybe the machine just needs a maintenance check due to it being an older machine. I wasn't sure if the advancement mechanism may be off or if it is another issue that doesn't seem apparent to me. Any help would be greatly appreciated. Thanks, Elliott Perow Juniata College Biology POE CONFIDENTIALITY NOTICE: The materials in this electronic mail transmission (including all attachments) are private and confidential and are the property of the sender. The information contained in the material is privileged and is intended only for the use of the named addressee(s). If you are not the intended addressee, be advised that any unauthorized disclosure, copying, distribution or the taking of any action in reliance on the contents of this material is strictly prohibited. If you have received this e-mail in error, please immediately notify the sender by replying to the e-mail, and then destroy it immediately. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] HT HistoDeck question...
Personally, I think it's a is a wrong answer, and that you are correct that b is a better answer. My students and I have found a couple of other questions that we thought had the wrong answer indicated in the study set. Peggy A. Wenk, HTL(ASCP)SLS -Original Message- From: Stephenson, Sheryl Sent: Thursday, October 03, 2013 7:21 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HT HistoDeck question... Hi, Please clarify why this answer to the HistoDeck study question is a) and not b). Here is the question: 'Frozen section slides cut from fresh, unfixed tissue specimens are optimally fixed in which of the following solutions? a) 37%-40% formaldehyde b) Cold acetone c) Acetic acid alcohol d) Alcoholic formalin Thanks, Sheryl Stephenson | Histology Technician ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] RE: Inconsistent Sections with Cryostat
I just attended Jan Minshew's workshop on cryostats at the NSH Symposium in Providence, RI, and she brought up something I had never thought of that causes thick and thin. If the handle that tightens the blade in the blade holder is over-tightened, the blade will become bowed, and that will cause thick-and-thin during sectioning. According to Jan, the handle should be tightened to be parallel to the angle of the blade holder slope. A lot of times, the handle can be pushed further back towards the back of the cryostat, beyond being parallel with the slope. The thought seems to be, if tight is good, then tighter is better, but not in this case. Peggy A. Wenk, HTL(ASCP)SLS -Original Message- From: Manfre, Philip Sent: Thursday, October 03, 2013 7:54 AM To: Perow, Elliott S (PEROWES10) ; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Inconsistent Sections with Cryostat I believe you may need to have the unit serviced. It sounds like something is not tight enough, perhaps the stage or blade holder unit. You said you secured everything which makes me think you have some issue with the cryostat itself. If a sharp blade, tightened blade and specimen, and varying the speed doesn't work, then you may need professional assistance form a service technician, especially if it is an older unit that has been moved around. Have you tried different temperatures? I don't think that is the problem but it is worth a try. Good luck! Cryosectioning can be an art in itself. Those decent sections are likely thicker than you intend, by the way. Classic thick and thin. Philip Manfre, B.A., HT (ASCP) Associate Principal Scientist Merck Research Laboratories WP45-251 PO Box 4 West Point, PA 19486 215-652-9750 215-993-0383 (fax) philip_man...@merck.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Perow, Elliott S (PEROWES10) Sent: Thursday, October 03, 2013 12:33 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Inconsistent Sections with Cryostat Hello All, I have a Leica Cryocut 1800 with a Reichert-Jung 2020 microtome that has sat for 8 years and went through a move from one academic building to another. I am trying to section brook trout brain, but am having difficulty getting consistent sections. I will get a good section and then the next section will be a very very thin shaving of usually just the top portion of the OCT block. I have tried adjusting the clearance angle, made sure the blade and specimen is secured, replaced the blade with a new one, have tried different sectioning speeds, different thicknesses of the section and still continually only get a decent section every other turn. I was wondering if anyone has had any similar experiences or if maybe the machine just needs a maintenance check due to it being an older machine. I wasn't sure if the advancement mechanism may be off or if it is another issue that doesn't seem apparent to me. Any help would be greatly appreciated. Thanks, Elliott Perow Juniata College Biology POE CONFIDENTIALITY NOTICE: The materials in this electronic mail transmission (including all attachments) are private and confidential and are the property of the sender. The information contained in the material is privileged and is intended only for the use of the named addressee(s). If you are not the intended addressee, be advised that any unauthorized disclosure, copying, distribution or the taking of any action in reliance on the contents of this material is strictly prohibited. If you have received this e-mail in error, please immediately notify the sender by replying to the e-mail, and then destroy it immediately. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] HT HistoDeck question...
For frozen cut sections, would the fixation also depend on what you plan on doing with it. For example, HE, Special Stain or IHC? Please correct if I am wrong. I think that is a trick question!!! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet- boun...@lists.utsouthwestern.edu] On Behalf Of Lee Peggy Wenk Sent: Thursday, October 03, 2013 8:16 AM To: Stephenson, Sheryl; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] HT HistoDeck question... Personally, I think it's a is a wrong answer, and that you are correct that b is a better answer. My students and I have found a couple of other questions that we thought had the wrong answer indicated in the study set. Peggy A. Wenk, HTL(ASCP)SLS -Original Message- From: Stephenson, Sheryl Sent: Thursday, October 03, 2013 7:21 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HT HistoDeck question... Hi, Please clarify why this answer to the HistoDeck study question is a) and not b). Here is the question: 'Frozen section slides cut from fresh, unfixed tissue specimens are optimally fixed in which of the following solutions? a) 37%-40% formaldehyde b) Cold acetone c) Acetic acid alcohol d) Alcoholic formalin Thanks, Sheryl Stephenson | Histology Technician ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message (including any attachments) may contain confidential, proprietary, privileged and/or private information. The information is intended to be for the use of the individual or entity designated above. If you are not the intended recipient of this message, please notify the sender immediately, and delete the message and any attachments. Any disclosure, reproduction, distribution or other use of this message or any attachments by an individual or entity other than the intended recipient is prohibited. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] HT HistoDeck question...
We fix HE's in 95% and our IHC protocol is acetone/alcohol fixation. Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Watson, Linda Sent: Thursday, October 03, 2013 8:40 AM To: Lee Peggy Wenk; Stephenson, Sheryl; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] HT HistoDeck question... For frozen cut sections, would the fixation also depend on what you plan on doing with it. For example, HE, Special Stain or IHC? Please correct if I am wrong. I think that is a trick question!!! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet- boun...@lists.utsouthwestern.edu] On Behalf Of Lee Peggy Wenk Sent: Thursday, October 03, 2013 8:16 AM To: Stephenson, Sheryl; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] HT HistoDeck question... Personally, I think it's a is a wrong answer, and that you are correct that b is a better answer. My students and I have found a couple of other questions that we thought had the wrong answer indicated in the study set. Peggy A. Wenk, HTL(ASCP)SLS -Original Message- From: Stephenson, Sheryl Sent: Thursday, October 03, 2013 7:21 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HT HistoDeck question... Hi, Please clarify why this answer to the HistoDeck study question is a) and not b). Here is the question: 'Frozen section slides cut from fresh, unfixed tissue specimens are optimally fixed in which of the following solutions? a) 37%-40% formaldehyde b) Cold acetone c) Acetic acid alcohol d) Alcoholic formalin Thanks, Sheryl Stephenson | Histology Technician ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message (including any attachments) may contain confidential, proprietary, privileged and/or private information. The information is intended to be for the use of the individual or entity designated above. If you are not the intended recipient of this message, please notify the sender immediately, and delete the message and any attachments. Any disclosure, reproduction, distribution or other use of this message or any attachments by an individual or entity other than the intended recipient is prohibited. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] The NSH was AMAZING!!! Congratulations to the 2013 Leadership, Education and Advocacy Award Winners!! From Pam Barker and RELIA Solutions!!
Hi Histonetters!!! How are you doing? The NSH was AMAZING! Providence was GORGEOUS The Speakers were BRILLIANT CONGRATULATIONS TO THE 2013 Leadership Education And Advocacy Award Winners: Histotechnologist of the Year-Wanda Jones J.B. McCormick Award- Peggy Wenk President's Award- Janet Dapson Lee G. Luna Foreign Travel Scholarship- M. Lamar Jones William Hacker Memorial Award- Anthony Wong Rosemary Donald Ostermeier Memorial Award- Christiane Coady Ventana IHC Award- Tiana Baskin Biogenex Standardization in IHC Award-David Davis Biogenex Standardization in ISH Award Dale Telgenhoff Dako Standardization of IHC Award-Jennifer Wright Anne Preece Award -Sarah Mack Leica Leadership in Management Award-Nirmala Amin Leica Leadership in Teaching Award- Elaine Basham Newcomer Helping Hand Award- Jean Mitchell Epitomics IHC Award- Melissa Downing Jules Elias Excellence in IHC Award- James Burchette Since I have returned to the office my phone has been ringing off the hook with exciting new opportunities. Here is a list of my current openings. All of these are full time permanent positions. My clients offer excellent compensation, benefits and relocation assistance. And they are ready to interview and hire right away! HISTOLOGY SUPERVISORS/MANAGERS: Histology Supervisor - Tallahassee, FL Histology Supervisor - West Palm Beach, FL Histology Supervisor - Seattle, WA Lead Pathology Tech - East of Columbus, OH HISTOTECHNICIANS/HISTOTECHNOLOGISTS: Histology Tech with Electron Microscopy - Southern CA Histotechnician Day shift -East of Columbus, OH Histotechnician Day shift - Tyler, TX Histotechnologist - Days - Seattle, WA Histology Tech - West Palm Beach, FL Histotechnician - Days - Harrisonburg, VA Histotechnician - Nights - Nashville, TN Senior Histotechnologist with FISH - Louisville, KY OTHER OPPORTUNITIES MT (ASCP) with Molecular experience- RTP, SC Of course I can't put all the information about these opportunities in an e-mail. So if you or anyone you know might be interested in hearing more about any of these positions or want help with a job search in another area please contact me. I can be reached at 866-607-3542 or rel...@earthlink.net. Remember it never hurts to look. Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: rel...@earthlink.net www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] HT HistoDeck question...
Thank you All! Sheryl Stephenson | Histology Technician -Original Message- From: Bernice Frederick [mailto:b-freder...@northwestern.edu] Sent: Thursday, October 03, 2013 9:43 AM To: Watson, Linda; Lee Peggy Wenk; Stephenson, Sheryl; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] HT HistoDeck question... We fix HE's in 95% and our IHC protocol is acetone/alcohol fixation. Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Watson, Linda Sent: Thursday, October 03, 2013 8:40 AM To: Lee Peggy Wenk; Stephenson, Sheryl; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] HT HistoDeck question... For frozen cut sections, would the fixation also depend on what you plan on doing with it. For example, HE, Special Stain or IHC? Please correct if I am wrong. I think that is a trick question!!! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet- boun...@lists.utsouthwestern.edu] On Behalf Of Lee Peggy Wenk Sent: Thursday, October 03, 2013 8:16 AM To: Stephenson, Sheryl; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] HT HistoDeck question... Personally, I think it's a is a wrong answer, and that you are correct that b is a better answer. My students and I have found a couple of other questions that we thought had the wrong answer indicated in the study set. Peggy A. Wenk, HTL(ASCP)SLS -Original Message- From: Stephenson, Sheryl Sent: Thursday, October 03, 2013 7:21 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HT HistoDeck question... Hi, Please clarify why this answer to the HistoDeck study question is a) and not b). Here is the question: 'Frozen section slides cut from fresh, unfixed tissue specimens are optimally fixed in which of the following solutions? a) 37%-40% formaldehyde b) Cold acetone c) Acetic acid alcohol d) Alcoholic formalin Thanks, Sheryl Stephenson | Histology Technician ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message (including any attachments) may contain confidential, proprietary, privileged and/or private information. The information is intended to be for the use of the individual or entity designated above. If you are not the intended recipient of this message, please notify the sender immediately, and delete the message and any attachments. Any disclosure, reproduction, distribution or other use of this message or any attachments by an individual or entity other than the intended recipient is prohibited. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] HT HistoDeck question...
I agree, there is probably more than one correct answer to this question, depending upon whether you are planning on doing stains for lipids, IHC, immunofluoresence or muscle enzymes. But I don't think (A) full strength 37-40% formaldehyde solution would ever be the correct answer. Unless you put a gauze in the bottom on the coplin jar, pour a little conc. formaldehyde on the gauze, put a lid on the coplin jar, and fix the section in formaldehyde vapors. But the question does say SOLUTION, not VAPOR. So I still think A is wrong. And most likely full strength acetic acid (C) is wrong - would eat the tissue off the slide. That leaves cold acetone (B) , which is good for some antibodies and some enzymes, or alcoholic formalin (D) which might be OK, but most of the time things either like alcohol and hate formalin, or they like formalin and don't like alcohol. So I would think most FS that we want to fix would not particularly like alcoholic formalin. And none of the solutions listed are good for lipids. So, given the question (with incomplete information) and the choices of answers, I would still side with (B) cold acetone. Now - a little aside - for the questions on the ASCP HT and HTL exams - if it is a new question, the people on the HT/HTL exam committee would be looking at it intensely before it goes on the exam for the first time. If the committee people are having problems answering it (like we are here), the question would be reworked until all the issues are resolved (such as putting in for lymph node IHC into the question). If it makes it past the committee, and the stats from the exam show that many people are having problems answering it, the question is pulled from the exam and is not used in the person's score. The question is then sent back to the HT/HTL exam committee, to try to figure out why examinees were having problems, and the question reworked again. As someone who has written exam questions at the school for 20+ years, I can tell you that it really is hard to write exam questions. I think I've covered everything in the question so that it is straight forward, and then half the students read something into the question that I never thought of, or come up with a written answer that I never considered. So I either have to throw out the question or give the point to the student, depending upon what's going on. And then mark the question for a re-write next year. And that doesn't include me marking the wrong answer on my master sheet. It happens! Peggy A. Wenk, HTL(ASCP)SLS -Original Message- From: Watson, Linda Sent: Thursday, October 03, 2013 9:40 AM To: Lee Peggy Wenk ; Stephenson, Sheryl ; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] HT HistoDeck question... For frozen cut sections, would the fixation also depend on what you plan on doing with it. For example, HE, Special Stain or IHC? Please correct if I am wrong. I think that is a trick question!!! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet- boun...@lists.utsouthwestern.edu] On Behalf Of Lee Peggy Wenk Sent: Thursday, October 03, 2013 8:16 AM To: Stephenson, Sheryl; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] HT HistoDeck question... Personally, I think it's a is a wrong answer, and that you are correct that b is a better answer. My students and I have found a couple of other questions that we thought had the wrong answer indicated in the study set. Peggy A. Wenk, HTL(ASCP)SLS -Original Message- From: Stephenson, Sheryl Sent: Thursday, October 03, 2013 7:21 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HT HistoDeck question... Hi, Please clarify why this answer to the HistoDeck study question is a) and not b). Here is the question: 'Frozen section slides cut from fresh, unfixed tissue specimens are optimally fixed in which of the following solutions? a) 37%-40% formaldehyde b) Cold acetone c) Acetic acid alcohol d) Alcoholic formalin Thanks, Sheryl Stephenson | Histology Technician ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message (including any attachments) may contain confidential, proprietary, privileged and/or private information. The information is intended to be for the use of the individual or entity designated above. If you are not the intended recipient of this message, please notify the sender immediately, and delete the message and any attachments. Any disclosure, reproduction, distribution or
Re: [Histonet] Vacuum and pressure in tissue processing
When tissue processing was manual there were some gadgets providing vacuum and those using it reported better results. The fact of the matter was that manual processing is so slow that anything you introduce will favor the process. Static tissue processors, i.e. those that only mover the specimens circumventing the manual transfer like the HistoKinete only improved processing if they were able to move the specimens, something they did by adding rotation inside the reagents vessels. Retort tissue processors introduced 3 novelties: vacuum, pressure and, most importantly, agitation that is nothing but empty/fill the whole retort every 20 minutes. This agitation is more important, as you point out, than the vacuum/pressure. René J. From: Teri Johnson tjohn...@gnf.org To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Sent: Wednesday, October 2, 2013 6:57 PM Subject: [Histonet] Vacuum and pressure in tissue processing Dear friends, I recall hearing at a conference (or maybe it was just a casual conversation by an expert during a NSH symposium break) that vacuum and pressure in tissue processing really accomplishes very little. I do believe that using heat and agitation of the solutions provides more activity kinetically and therefore makes processing more efficient. Can someone affirm or deny the efficacy of vacuum and/or pressure in tissue processing, please? Thank you, as always, for your wisdom. Teri Johnson Manager, Histology Genomics Institute for Novartis Research Foundation San Diego, CA 858-332-4752 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] HT HistoDeck question...
Honestly I think this all depends on what they are actually asking. Are they asking what you fix the tissue in after the frozen is complete and you need to submit the remaining tissue for routine processing? If so, then A is the correct answer. Are they asking what you fix the tissue to the slide with to stain the frozen section for immediate diagnosis? If so, then the answer can be several possibilities depending on what you are going to be staining/ the process you will be staining with. I have worked in several different hospitals and each have a different protocol for there frozen section staining. In order to fix the tissue to the slide I have used 95% alcohol and acidic acid alcohol. The only time I have used formaldehyde is when I use the vapors for fat staining. I think this might be a question to take up with ASCP. They are the ones administering the test. Emily K. McKenzie BS, HT(ASCP) Memorial Medical Center│701 North First Street│Springfield, IL 62781 Ph: 217-788-3991│email: mckenzie.em...@mhsil.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lee Peggy Wenk Sent: Thursday, October 03, 2013 9:22 AM To: Watson, Linda; Stephenson, Sheryl; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] HT HistoDeck question... I agree, there is probably more than one correct answer to this question, depending upon whether you are planning on doing stains for lipids, IHC, immunofluoresence or muscle enzymes. But I don't think (A) full strength 37-40% formaldehyde solution would ever be the correct answer. Unless you put a gauze in the bottom on the coplin jar, pour a little conc. formaldehyde on the gauze, put a lid on the coplin jar, and fix the section in formaldehyde vapors. But the question does say SOLUTION, not VAPOR. So I still think A is wrong. And most likely full strength acetic acid (C) is wrong - would eat the tissue off the slide. That leaves cold acetone (B) , which is good for some antibodies and some enzymes, or alcoholic formalin (D) which might be OK, but most of the time things either like alcohol and hate formalin, or they like formalin and don't like alcohol. So I would think most FS that we want to fix would not particularly like alcoholic formalin. And none of the solutions listed are good for lipids. So, given the question (with incomplete information) and the choices of answers, I would still side with (B) cold acetone. Now - a little aside - for the questions on the ASCP HT and HTL exams - if it is a new question, the people on the HT/HTL exam committee would be looking at it intensely before it goes on the exam for the first time. If the committee people are having problems answering it (like we are here), the question would be reworked until all the issues are resolved (such as putting in for lymph node IHC into the question). If it makes it past the committee, and the stats from the exam show that many people are having problems answering it, the question is pulled from the exam and is not used in the person's score. The question is then sent back to the HT/HTL exam committee, to try to figure out why examinees were having problems, and the question reworked again. As someone who has written exam questions at the school for 20+ years, I can tell you that it really is hard to write exam questions. I think I've covered everything in the question so that it is straight forward, and then half the students read something into the question that I never thought of, or come up with a written answer that I never considered. So I either have to throw out the question or give the point to the student, depending upon what's going on. And then mark the question for a re-write next year. And that doesn't include me marking the wrong answer on my master sheet. It happens! Peggy A. Wenk, HTL(ASCP)SLS -Original Message- From: Watson, Linda Sent: Thursday, October 03, 2013 9:40 AM To: Lee Peggy Wenk ; Stephenson, Sheryl ; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] HT HistoDeck question... For frozen cut sections, would the fixation also depend on what you plan on doing with it. For example, HE, Special Stain or IHC? Please correct if I am wrong. I think that is a trick question!!! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet- boun...@lists.utsouthwestern.edu] On Behalf Of Lee Peggy Wenk Sent: Thursday, October 03, 2013 8:16 AM To: Stephenson, Sheryl; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] HT HistoDeck question... Personally, I think it's a is a wrong answer, and that you are correct that b is a better answer. My students and I have found a couple of other questions that we thought had the wrong answer indicated in the study set. Peggy A. Wenk, HTL(ASCP)SLS -Original Message- From: Stephenson, Sheryl Sent: Thursday, October 03, 2013 7:21 AM To:
[Histonet] Microtomes
Dear Histonians I'm in the market (please, no vendors) for a new microtome. I'd like an automated/manual type. What is on the market these days that is worthy? I know about Leica, but it's been many years since I've looked for these instruments and would love to know what you like and don't like about your microtomes? Respectfully, Bruce Gapinsk HT (ASCP) Chief Histologist Marin Medical Laboratories PathGroup SF Important Notice: This e-mail is intended for the use of the person to whom it is addressed and may contain information that is privileged and confidential. If you are not the intended recipient, any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this e-mail in error, please destroy this message and contact the Security Officer at PathGroup, Inc immediately at 615-562-9255. Thank you ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] HT HistoDeck question...
I'd go with A, but it really depends on what you are going to do with the sections after fixation. In the protocol for Oil Red O in Freida's second edition (that I use almost daily combined with some steps from PolyScientific's ORO protocol), step #1 says to fix in 40% formaldehyde. Doesn't say vapors so I plunk the slides in the liquid 40%. I have fixed frozen sections for regular HE and other stains as well in 40% formaldehyde and they come out beautiful. Unless there is some specific request or reason to use cold acetone or alcohol this is what I use. Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cellular and Molecular Medicine Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algra...@email.arizona.edumailto:algra...@email.arizona.edu Tel: 520.626.4415 Fax: 520.626.2097 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Microtomes
Still Leica René J. From: Bruce Gapinski bgapin...@pathgroup.com To: 'histonet@lists.utsouthwestern.edu' histonet@lists.utsouthwestern.edu Sent: Thursday, October 3, 2013 10:46 AM Subject: [Histonet] Microtomes Dear Histonians I'm in the market (please, no vendors) for a new microtome. I'd like an automated/manual type. What is on the market these days that is worthy? I know about Leica, but it's been many years since I've looked for these instruments and would love to know what you like and don't like about your microtomes? Respectfully, Bruce Gapinsk HT (ASCP) Chief Histologist Marin Medical Laboratories PathGroup SF Important Notice: This e-mail is intended for the use of the person to whom it is addressed and may contain information that is privileged and confidential. If you are not the intended recipient, any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this e-mail in error, please destroy this message and contact the Security Officer at PathGroup, Inc immediately at 615-562-9255. Thank you ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Microtomes
Still don't have use for fully automated. 1st microm , 2nd Leica Opinion only Sent from my Verizon Wireless 4G LTE Smartphone - Reply message - From: Bruce Gapinski bgapin...@pathgroup.com To: 'histonet@lists.utsouthwestern.edu' histonet@lists.utsouthwestern.edu Subject: [Histonet] Microtomes Date: Thu, Oct 3, 2013 10:46 am Dear Histonians I'm in the market (please, no vendors) for a new microtome. I'd like an automated/manual type. What is on the market these days that is worthy? I know about Leica, but it's been many years since I've looked for these instruments and would love to know what you like and don't like about your microtomes? Respectfully, Bruce Gapinsk HT (ASCP) Chief Histologist Marin Medical Laboratories PathGroup SF Important Notice: This e-mail is intended for the use of the person to whom it is addressed and may contain information that is privileged and confidential. If you are not the intended recipient, any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this e-mail in error, please destroy this message and contact the Security Officer at PathGroup, Inc immediately at 615-562-9255. Thank you ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Microtomes
Leica. Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bruce Gapinski Sent: Thursday, October 03, 2013 7:47 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Microtomes Dear Histonians I'm in the market (please, no vendors) for a new microtome. I'd like an automated/manual type. What is on the market these days that is worthy? I know about Leica, but it's been many years since I've looked for these instruments and would love to know what you like and don't like about your microtomes? Respectfully, Bruce Gapinsk HT (ASCP) Chief Histologist Marin Medical Laboratories PathGroup SF Important Notice: This e-mail is intended for the use of the person to whom it is addressed and may contain information that is privileged and confidential. If you are not the intended recipient, any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this e-mail in error, please destroy this message and contact the Security Officer at PathGroup, Inc immediately at 615-562-9255. Thank you ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Microtomes
I would go with Leica. I am getting them for my lab. Haley H. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bea DeBrosse-Serra Sent: Thursday, October 03, 2013 9:23 AM To: 'Bruce Gapinski'; 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] RE: Microtomes Leica. Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bruce Gapinski Sent: Thursday, October 03, 2013 7:47 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Microtomes Dear Histonians I'm in the market (please, no vendors) for a new microtome. I'd like an automated/manual type. What is on the market these days that is worthy? I know about Leica, but it's been many years since I've looked for these instruments and would love to know what you like and don't like about your microtomes? Respectfully, Bruce Gapinsk HT (ASCP) Chief Histologist Marin Medical Laboratories PathGroup SF Important Notice: This e-mail is intended for the use of the person to whom it is addressed and may contain information that is privileged and confidential. If you are not the intended recipient, any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this e-mail in error, please destroy this message and contact the Security Officer at PathGroup, Inc immediately at 615-562-9255. Thank you ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] HT HistoDeck question...
We also use this oil red O method and use the 40% formaldehyde. The questions lacks enough information to correctly answer it. I am sure the author of the question had something in mind and other options didn't occur to him/her at the time. Jennifer From: Grantham, Andrea L - (algranth) algra...@email.arizona.edu To: Cc: HISTONET histonet@lists.utsouthwestern.edu Date: 10/03/2013 08:33 AM Subject:Re: [Histonet] HT HistoDeck question... Sent by:histonet-boun...@lists.utsouthwestern.edu I'd go with A, but it really depends on what you are going to do with the sections after fixation. In the protocol for Oil Red O in Freida's second edition (that I use almost daily combined with some steps from PolyScientific's ORO protocol), step #1 says to fix in 40% formaldehyde. Doesn't say vapors so I plunk the slides in the liquid 40%. I have fixed frozen sections for regular HE and other stains as well in 40% formaldehyde and they come out beautiful. Unless there is some specific request or reason to use cold acetone or alcohol this is what I use. Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cellular and Molecular Medicine Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algra...@email.arizona.edumailto:algra...@email.arizona.edu Tel: 520.626.4415 Fax: 520.626.2097 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] RE: Microtomes
Thermo Shandon Finesse is my favorite. Manual version requires oiling which is easy and gives you a good reason to keep it clean inside. I got one for about 6,000. The high end automatic version is the Finesse ME+ and is the best microtome I have ever seen. Someone that has never cut before could sit down and get perfect sections on their first block. The quote i got for that was about 12,000 which was too much for my lab. Tony Auge HTL (ASCP) QIHC Cell: (651) 373-4768 Email: tony.a...@gmail.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Microtomes
Leica always Cheryl A. Miller HT ASCP cm Histology Supervisor Hygiene Officer Physicians Laboratory, P.C. 4140 F St. Omaha , NE. 68117 402 731 4145 ext. 532 Cell 402 493 0403 From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa [rjbu...@yahoo.com] Sent: Thursday, October 03, 2013 10:48 AM To: Bruce Gapinski; 'histonet@lists.utsouthwestern.edu' Subject: Re: [Histonet] Microtomes Still Leica René J. From: Bruce Gapinski bgapin...@pathgroup.com To: 'histonet@lists.utsouthwestern.edu' histonet@lists.utsouthwestern.edu Sent: Thursday, October 3, 2013 10:46 AM Subject: [Histonet] Microtomes Dear Histonians I'm in the market (please, no vendors) for a new microtome. I'd like an automated/manual type. What is on the market these days that is worthy? I know about Leica, but it's been many years since I've looked for these instruments and would love to know what you like and don't like about your microtomes? Respectfully, Bruce Gapinsk HT (ASCP) Chief Histologist Marin Medical Laboratories PathGroup SF Important Notice: This e-mail is intended for the use of the person to whom it is addressed and may contain information that is privileged and confidential. If you are not the intended recipient, any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this e-mail in error, please destroy this message and contact the Security Officer at PathGroup, Inc immediately at 615-562-9255. Thank you ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] HT HistoDeck question...
For those of you who use the 40% formaldehyde, how long is you fixation time on frozen slides? We use 10% NBF with 1 minute to fix, but sometimes it gets hectic if you have multiple frozens all at once. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jennifer MacDonald Sent: Thursday, October 03, 2013 2:04 PM To: Grantham, Andrea L - (algranth) Cc: HISTONET; histonet-boun...@lists.utsouthwestern.edu Subject: Re: [Histonet] HT HistoDeck question... We also use this oil red O method and use the 40% formaldehyde. The questions lacks enough information to correctly answer it. I am sure the author of the question had something in mind and other options didn't occur to him/her at the time. Jennifer From: Grantham, Andrea L - (algranth) algra...@email.arizona.edu To: Cc: HISTONET histonet@lists.utsouthwestern.edu Date: 10/03/2013 08:33 AM Subject:Re: [Histonet] HT HistoDeck question... Sent by:histonet-boun...@lists.utsouthwestern.edu I'd go with A, but it really depends on what you are going to do with the sections after fixation. In the protocol for Oil Red O in Freida's second edition (that I use almost daily combined with some steps from PolyScientific's ORO protocol), step #1 says to fix in 40% formaldehyde. Doesn't say vapors so I plunk the slides in the liquid 40%. I have fixed frozen sections for regular HE and other stains as well in 40% formaldehyde and they come out beautiful. Unless there is some specific request or reason to use cold acetone or alcohol this is what I use. Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cellular and Molecular Medicine Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algra...@email.arizona.edumailto:algra...@email.arizona.edu Tel: 520.626.4415 Fax: 520.626.2097 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] C3d by IHC on FFPE human specimens
Does anyone in Histoland know of a reference lab that offers this? Thanks, Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] HT HistoDeck question...
It would dissolve the fat if you used acetone wouldnt it? Without knowing the tissue type or stain, the answer is A. All other choices dissolve fat I think. Good luck on your exam! Sent from my iPhone On Oct 3, 2013, at 8:15 AM, Lee Peggy Wenk lpw...@sbcglobal.net wrote: Personally, I think it's a is a wrong answer, and that you are correct that b is a better answer. My students and I have found a couple of other questions that we thought had the wrong answer indicated in the study set. Peggy A. Wenk, HTL(ASCP)SLS -Original Message- From: Stephenson, Sheryl Sent: Thursday, October 03, 2013 7:21 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HT HistoDeck question... Hi, Please clarify why this answer to the HistoDeck study question is a) and not b). Here is the question: 'Frozen section slides cut from fresh, unfixed tissue specimens are optimally fixed in which of the following solutions? a) 37%-40% formaldehyde b) Cold acetone c) Acetic acid alcohol d) Alcoholic formalin Thanks, Sheryl Stephenson | Histology Technician ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
AW: [Histonet] HT HistoDeck question...
We use 36-40% formaldehyde for a minimal time of 2-3 dips for fast frozen HE. On the other side the slides stand in the solution as long as all frozens are already cut. Commercial formaldehyde contents a good part of methanol (a MSDS says 5-15%). So fast fixation is a combination of formaldehyde and alcohol fixation. Additional we use Harris hematoxylin, that's also mainly made of ethanol. So in my opinion all together fixation is rather due to alcohol in this way. Gudrun -Ursprüngliche Nachricht- Von: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von Rathborne, Toni Gesendet: Donnerstag, 03. Oktober 2013 20:34 An: 'Jennifer MacDonald'; Grantham, Andrea L - (algranth) Cc: HISTONET; histonet-boun...@lists.utsouthwestern.edu Betreff: RE: [Histonet] HT HistoDeck question... For those of you who use the 40% formaldehyde, how long is you fixation time on frozen slides? We use 10% NBF with 1 minute to fix, but sometimes it gets hectic if you have multiple frozens all at once. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jennifer MacDonald Sent: Thursday, October 03, 2013 2:04 PM To: Grantham, Andrea L - (algranth) Cc: HISTONET; histonet-boun...@lists.utsouthwestern.edu Subject: Re: [Histonet] HT HistoDeck question... We also use this oil red O method and use the 40% formaldehyde. The questions lacks enough information to correctly answer it. I am sure the author of the question had something in mind and other options didn't occur to him/her at the time. Jennifer From: Grantham, Andrea L - (algranth) algra...@email.arizona.edu To: Cc: HISTONET histonet@lists.utsouthwestern.edu Date: 10/03/2013 08:33 AM Subject:Re: [Histonet] HT HistoDeck question... Sent by:histonet-boun...@lists.utsouthwestern.edu I'd go with A, but it really depends on what you are going to do with the sections after fixation. In the protocol for Oil Red O in Freida's second edition (that I use almost daily combined with some steps from PolyScientific's ORO protocol), step #1 says to fix in 40% formaldehyde. Doesn't say vapors so I plunk the slides in the liquid 40%. I have fixed frozen sections for regular HE and other stains as well in 40% formaldehyde and they come out beautiful. Unless there is some specific request or reason to use cold acetone or alcohol this is what I use. Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cellular and Molecular Medicine Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algra...@email.arizona.edumailto:algra...@email.arizona.edu Tel: 520.626.4415 Fax: 520.626.2097 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Question about fixation terminology
Hello - Our research group does a fair amount of autoradiography with frozen sections and we sometimes perform IHC or routine stains. I am not a histologist (nor do we have one in our current group), so I assumed that the correct answer was alcoholic formalin, because the other options were either inappropriate or not fixatives. I was taught (by an old-school cell biologist) that both alcohol and acetone act as dehydrating agents when used on frozen sections, and are not true fixatives, because they don't cross-link proteins. Real fixatives don't just preserve against decay, they also modify the tissue (i.e., cross-linking or denaturing proteins). I am pretty sure I was taught that acetone does not fix tissue, and that fixing tissue to a slide is an imprecise/slang term derived from affix. (This was at least a decade ago, so my memory could be faulty.) How is acetone a fixative? I thought it just replaced water, and preserved the structure of frozen sections by drying them out. (Please be kind - I'm not a histologist. I've sat at the cryostat sectioning mouse brains, and I know when we use it, but I don't understand what the acetone actually does.) How do you describe the difference between preserving tissue by drying (with acetone) and cross-linking the proteins (with alcoholic formalin)? I would really appreciate clarification from the guru's on HistoNet. I no longer spend much time in the lab, but I edit our manuscripts, so try make sure we use the correct terms in describing how the work is done. (Some reviewers get picky about terminology.) Thanks, Lynne Jones Lab Manager Radiochemistry Research Group Radiological Chemistry and Imaging Lab Washington University School of Medicine St. Louis, MO Message: 2 Date: Thu, 3 Oct 2013 10:21:42 -0400 From: Lee Peggy Wenk lpw...@sbcglobal.netmailto:lpw...@sbcglobal.net Subject: Re: [Histonet] HT HistoDeck question... To: Watson, Linda linda.wat...@bms.commailto:linda.wat...@bms.com, Stephenson, Sheryl sstephen...@lifecell.commailto:sstephen...@lifecell.com, histonet@lists.utsouthwestern.edumailto:histonet@lists.utsouthwestern.edu Message-ID: 335B9C2DCE414D1CB831DCCB07DEDC94@HP2010 Content-Type: text/plain; format=flowed; charset=iso-8859-1; reply-type=original I agree, there is probably more than one correct answer to this question, depending upon whether you are planning on doing stains for lipids, IHC, immunofluoresence or muscle enzymes. But I don't think (A) full strength 37-40% formaldehyde solution would ever be the correct answer. Unless you put a gauze in the bottom on the coplin jar, pour a little conc. formaldehyde on the gauze, put a lid on the coplin jar, and fix the section in formaldehyde vapors. But the question does say SOLUTION, not VAPOR. So I still think A is wrong. And most likely full strength acetic acid (C) is wrong - would eat the tissue off the slide. That leaves cold acetone (B) , which is good for some antibodies and some enzymes, or alcoholic formalin (D) which might be OK, but most of the time things either like alcohol and hate formalin, or they like formalin and don't like alcohol. So I would think most FS that we want to fix would not particularly like alcoholic formalin. And none of the solutions listed are good for lipids. So, given the question (with incomplete information) and the choices of answers, I would still side with (B) cold acetone. Now - a little aside - for the questions on the ASCP HT and HTL exams - if it is a new question, the people on the HT/HTL exam committee would be looking at it intensely before it goes on the exam for the first time. If the committee people are having problems answering it (like we are here), the question would be reworked until all the issues are resolved (such as putting in for lymph node IHC into the question). If it makes it past the committee, and the stats from the exam show that many people are having problems answering it, the question is pulled from the exam and is not used in the person's score. The question is then sent back to the HT/HTL exam committee, to try to figure out why examinees were having problems, and the question reworked again. As someone who has written exam questions at the school for 20+ years, I can tell you that it really is hard to write exam questions. I think I've covered everything in the question so that it is straight forward, and then half the students read something into the question that I never thought of, or come up with a written answer that I never considered. So I either have to throw out the question or give the point to the student, depending upon what's going on. And then mark the question for a re-write next year. And that doesn't include me marking the wrong answer on my master sheet. It happens! Peggy A. Wenk, HTL(ASCP)SLS -Original Message- From: Watson, Linda Sent:
RE: [Histonet] Question about fixation terminology
Surely I will find those that disagree with this post, however what I was classically trained about fixation categories generally falls within the information below...which I took the liberty of reposting here since it is pretty clear straightforward from Leica. http://www.leicabiosystems.com/pathologyleaders/fixation-and-fixatives-1-the-process-of-fixation-and-the-nature-of-fixatives/ I hope this will help. Traditionally fixing agents were termed “coagulant” or “non-coagulant” based on their effect on soluble proteins in solution. 7 Coagulant fixatives were said to result in a permeable meshwork of protein strands whereas non-coagulant fixatives which are additive in nature, formed extensive cross-links producing a less permeable gel. These terms are still encountered in modern histological literature but a more systematic approach has recently been taken to classification. 2 There are two major mechanisms which are important in fixation of proteins and protein complexes: denaturation, and addition and cross-link formation. Denaturation: Most commonly this effect is induced by dehydrants such as the alcohols or acetone. These reagents remove and replace free water in cells and tissues and cause a change in the tertiary structure of proteins by destabilizing hydrophobic bonding. Hydrophobic areas, frequently found on the inside of protein molecules, are released from the repulsion of water and become free to occupy a greater area. hydrophilic areas of protein water molecules are loosely bound by hydrogen bonds and removal of water also destabilizes these bonds. The changes produced in the conformation of the protein molecules cause a change in the solubility of the protein, rendering water soluble proteins insoluble, a change that is largely irreversible if the protein is returned to an aqueous environment. 2, 7 Addition and cross-link formation: The non-coagulant fixing agents chemically react with proteins and other cell and tissue components, becoming bound to them by addition and forming inter-molecular and intra-molecular cross-links. Because these agents are reactive compounds they bind to a variety of chemical groups in tissues, often affecting the charge at the site of attachment. This can have an effect on the subsequent staining characteristics of a particular protein as well as altering its molecular conformation and thus its solubility . For further and more in depth discussion of formalin fixation ( and the article I am usually referred to in order to make corrections to my own chemistry in publications) is 1985!! Fox, et al. Formaldehyde Fixation, Journal of Histochemistry and Cytochemistry, vol. 33, No. 8. pp. 845-855. pdf available at http://www.gofindpdf.com/readpdf/formaldehyde-fixation.html Joelle Weaver MAOM, HTL (ASCP) QIHC From: jone...@mir.wustl.edu To: histonet@lists.utsouthwestern.edu Date: Thu, 3 Oct 2013 19:32:51 + CC: Subject: [Histonet] Question about fixation terminology Hello - Our research group does a fair amount of autoradiography with frozen sections and we sometimes perform IHC or routine stains. I am not a histologist (nor do we have one in our current group), so I assumed that the correct answer was alcoholic formalin, because the other options were either inappropriate or not fixatives. I was taught (by an old-school cell biologist) that both alcohol and acetone act as dehydrating agents when used on frozen sections, and are not true fixatives, because they don't cross-link proteins. Real fixatives don't just preserve against decay, they also modify the tissue (i.e., cross-linking or denaturing proteins). I am pretty sure I was taught that acetone does not fix tissue, and that fixing tissue to a slide is an imprecise/slang term derived from affix. (This was at least a decade ago, so my memory could be faulty.) How is acetone a fixative? I thought it just replaced water, and preserved the structure of frozen sections by drying them out. (Please be kind - I'm not a histologist. I've sat at the cryostat sectioning mouse brains, and I know when we use it, but I don't understand what the acetone actually does.) How do you describe the difference between preserving tissue by drying (with acetone) and cross-linking the proteins (with alcoholic formalin)? I would really appreciate clarification from the guru's on HistoNet. I no longer spend much time in the lab, but I edit our manuscripts, so try make sure we use the correct terms in describing how the work is done. (Some reviewers get picky about terminology.) Thanks, Lynne Jones Lab Manager Radiochemistry Research Group Radiological Chemistry and Imaging Lab Washington University School of Medicine St. Louis, MO Message: 2 Date: Thu, 3 Oct 2013 10:21:42 -0400 From: Lee Peggy Wenk lpw...@sbcglobal.netmailto:lpw...@sbcglobal.net Subject: Re: [Histonet] HT
[Histonet] RE: Question about fixation terminology
http://www.ihcworld.com/_protocols/general_ICC/fixation.htm This article specifically addresses the differences. You are right about the cross linking, but alcohol and acetone are still considered fixatives. Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jones, Lynne Sent: Thursday, October 03, 2013 2:33 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Question about fixation terminology Hello - Our research group does a fair amount of autoradiography with frozen sections and we sometimes perform IHC or routine stains. I am not a histologist (nor do we have one in our current group), so I assumed that the correct answer was alcoholic formalin, because the other options were either inappropriate or not fixatives. I was taught (by an old-school cell biologist) that both alcohol and acetone act as dehydrating agents when used on frozen sections, and are not true fixatives, because they don't cross-link proteins. Real fixatives don't just preserve against decay, they also modify the tissue (i.e., cross-linking or denaturing proteins). I am pretty sure I was taught that acetone does not fix tissue, and that fixing tissue to a slide is an imprecise/slang term derived from affix. (This was at least a decade ago, so my memory could be faulty.) How is acetone a fixative? I thought it just replaced water, and preserved the structure of frozen sections by drying them out. (Please be kind - I'm not a histologist. I've sat at the cryostat sectioning mouse brains, and I know when we use it, but I don't understand what the acetone actually does.) How do you describe the difference between preserving tissue by drying (with acetone) and cross-linking the proteins (with alcoholic formalin)? I would really appreciate clarification from the guru's on HistoNet. I no longer spend much time in the lab, but I edit our manuscripts, so try make sure we use the correct terms in describing how the work is done. (Some reviewers get picky about terminology.) Thanks, Lynne Jones Lab Manager Radiochemistry Research Group Radiological Chemistry and Imaging Lab Washington University School of Medicine St. Louis, MO Message: 2 Date: Thu, 3 Oct 2013 10:21:42 -0400 From: Lee Peggy Wenk lpw...@sbcglobal.netmailto:lpw...@sbcglobal.net Subject: Re: [Histonet] HT HistoDeck question... To: Watson, Linda linda.wat...@bms.commailto:linda.wat...@bms.com, Stephenson, Sheryl sstephen...@lifecell.commailto:sstephen...@lifecell.com, histonet@lists.utsouthwestern.edumailto:histonet@lists.utsouthwestern.edu Message-ID: 335B9C2DCE414D1CB831DCCB07DEDC94@HP2010 Content-Type: text/plain; format=flowed; charset=iso-8859-1; reply-type=original I agree, there is probably more than one correct answer to this question, depending upon whether you are planning on doing stains for lipids, IHC, immunofluoresence or muscle enzymes. But I don't think (A) full strength 37-40% formaldehyde solution would ever be the correct answer. Unless you put a gauze in the bottom on the coplin jar, pour a little conc. formaldehyde on the gauze, put a lid on the coplin jar, and fix the section in formaldehyde vapors. But the question does say SOLUTION, not VAPOR. So I still think A is wrong. And most likely full strength acetic acid (C) is wrong - would eat the tissue off the slide. That leaves cold acetone (B) , which is good for some antibodies and some enzymes, or alcoholic formalin (D) which might be OK, but most of the time things either like alcohol and hate formalin, or they like formalin and don't like alcohol. So I would think most FS that we want to fix would not particularly like alcoholic formalin. And none of the solutions listed are good for lipids. So, given the question (with incomplete information) and the choices of answers, I would still side with (B) cold acetone. Now - a little aside - for the questions on the ASCP HT and HTL exams - if it is a new question, the people on the HT/HTL exam committee would be looking at it intensely before it goes on the exam for the first time. If the committee people are having problems answering it (like we are here), the question would be reworked until all the issues are resolved (such as putting in for lymph node IHC into the question). If it makes it past the committee, and the stats from the exam show that many people are having problems answering it, the question is pulled from the exam and is not used in the person's score. The question is then sent back to the HT/HTL exam committee, to try to figure out why examinees were having problems, and the question reworked again. As someone who has written exam questions at
[Histonet] Bodian
Hi Thanks to every one that sent me Acid Phosphotase procedure for muscle biopsies. I also need some help with the bodian stain, we are not getting any staining on the slides, we have a procedure that they used years ago and now we can't get it working (we did purchase new chemicals) and we also tried a stain kit with very little results. Thanks again Helene Upstate Medical University ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Proteinase K treatment
Hello histoneters! This is my first time using the net. I hope to find here some answers to my problem. I am working with free floating sections embedded in gelatin. I need to use Proteinase K to detect alpha- synuclein aggregates in brain tissue. I tried concentrations in the range of 1 Ug/ml to 5Ug/ml, incubation time is 10 minutes at room temperature. I noticed that after several rinses the Proteinase K keeps being active, because the gelatin in the sections gets sticky and even disappears overtime. I did several rinses (4 X 10 min each) and I am afraid that the antibody will get degraded in the overnight incubation. How can I stop this enzymatic reaction? I will truly appreciate any insights, Thank you! Claudia N. Segovia Senior Neurohistologist Antibody Specialist NeuroScience Associates 10915 Lake Ridge Drive Knoxville, TN 37934 865-675-2245 csego...@nsalabs.com STATEMENT OF CONFIDENTIALITY: The information contained in this electronic message and any attachments are intended for the exclusive use of the addressee(s) and may contain confidential or privileged information. If you are not the intended recipient, or the person responsible for delivering email to the intended recipient, be advised you have received this message in error and any use, dissemination, forwarding, printing or copying is strictly prohibited. Please notify NeuroScience Associates immediately at 865-675-2245 or at csego...@nsalabs.com, and destroy all copies of this message and any attachments. You will be reimbursed for reasonable costs incurred in notifying us. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] HT HistoDeck question...
the protocol calls for 1 minute in the 40% formaldehyde and then rinse the sections well. From: Rathborne, Toni trathbo...@somerset-healthcare.com To: 'Jennifer MacDonald' jmacdon...@mtsac.edu, Grantham, Andrea L - (algranth) algra...@email.arizona.edu Cc: HISTONET histonet@lists.utsouthwestern.edu, histonet-boun...@lists.utsouthwestern.edu histonet-boun...@lists.utsouthwestern.edu Date: 10/03/2013 11:42 AM Subject:RE: [Histonet] HT HistoDeck question... Sent by:histonet-boun...@lists.utsouthwestern.edu For those of you who use the 40% formaldehyde, how long is you fixation time on frozen slides? We use 10% NBF with 1 minute to fix, but sometimes it gets hectic if you have multiple frozens all at once. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [ mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jennifer MacDonald Sent: Thursday, October 03, 2013 2:04 PM To: Grantham, Andrea L - (algranth) Cc: HISTONET; histonet-boun...@lists.utsouthwestern.edu Subject: Re: [Histonet] HT HistoDeck question... We also use this oil red O method and use the 40% formaldehyde. The questions lacks enough information to correctly answer it. I am sure the author of the question had something in mind and other options didn't occur to him/her at the time. Jennifer From: Grantham, Andrea L - (algranth) algra...@email.arizona.edu To: Cc: HISTONET histonet@lists.utsouthwestern.edu Date: 10/03/2013 08:33 AM Subject:Re: [Histonet] HT HistoDeck question... Sent by:histonet-boun...@lists.utsouthwestern.edu I'd go with A, but it really depends on what you are going to do with the sections after fixation. In the protocol for Oil Red O in Freida's second edition (that I use almost daily combined with some steps from PolyScientific's ORO protocol), step #1 says to fix in 40% formaldehyde. Doesn't say vapors so I plunk the slides in the liquid 40%. I have fixed frozen sections for regular HE and other stains as well in 40% formaldehyde and they come out beautiful. Unless there is some specific request or reason to use cold acetone or alcohol this is what I use. Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cellular and Molecular Medicine Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algra...@email.arizona.edumailto:algra...@email.arizona.edu Tel: 520.626.4415 Fax: 520.626.2097 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE:Gram stain (Mesru T) Histonet Digest, Vol 119, Issue 4
We perform Gram Twort for our gram stains. It is essentially the same as the gram stain used in microbiology except that we counter stain with a neutral red fast green which is very effective. Cheers Cate Cate Hardy Senior Technical Officer Veterinary Diagnostic Laboratory School of Animal and Veterinary Sciences Locked bag 588 Charles Sturt University Wagga Wagga NSW 2678 T: 02 69334000 Email: cha...@csu.edu.au Email: v...@csu.edu.au -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histonet-requ...@lists.utsouthwestern.edu Sent: Friday, 4 October 2013 12:07 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 119, Issue 4 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than Re: Contents of Histonet digest... Today's Topics: 1. Gram stain (Mesru T) 2. Benchmark Ultra troubles (Gudrun Lang) 3. Re: RE: MOHS IPs (Kim Donadio) 4. Re: IHC turnaround time (Kim Donadio) 5. Re: Gram stain (Jennifer MacDonald) 6. Hi (Helene Degan) 7. RE: Validating new Benchmark instrument (Eytalis, Robert A) 8. need lever clamp for blade of leica microtome (Patricia F Lott) 9. Re: Shipping to Countries Outside of the US (Jan Shivers) 10. RE: Histotechnologist, (ASCP, QIHC certified) and Pathology assistant position in Seattle area (Yan Liang) 11. Vacuum and pressure in tissue processing (Teri Johnson) 12. Trivew (Gerald Davydov) 13. Inconsistent Sections with Cryostat (Perow, Elliott S (PEROWES10)) 14. HT HistoDeck question... (Stephenson, Sheryl) 15. RE: Inconsistent Sections with Cryostat (Manfre, Philip) 16. Re: HT HistoDeck question... (Lee Peggy Wenk) 17. Re: RE: Inconsistent Sections with Cryostat (Lee Peggy Wenk) 18. RE: HT HistoDeck question... (Watson, Linda) 19. RE: HT HistoDeck question... (Bernice Frederick) 20. The NSH was AMAZING!!! Congratulations to the 2013 Leadership, Education and Advocacy Award Winners!! From Pam Barker and RELIA Solutions!! (Pam Barker) -- Message: 1 Date: Wed, 2 Oct 2013 13:11:22 -0400 From: Mesru T turke...@gmail.com Subject: [Histonet] Gram stain To: histonet@lists.utsouthwestern.edu Message-ID: CACf3QnTkAREiduT-jCBZ6CGbd6Bn0qudF4cM6FE1F9TVgYE5=a...@mail.gmail.com Content-Type: text/plain; charset=ISO-8859-1 Hi All, Does anybody have a protocol for Gram stain on FFPE mouse intestine sections. We are looking to distinguish between Klebsiella and Vancomycin Resistant Enterococcus (VRE). I would like to avoid using Picric acid as some protocols suggest. Thanks in advance. -- Message: 2 Date: Wed, 2 Oct 2013 19:38:56 +0200 From: Gudrun Lang gu.l...@gmx.at Subject: [Histonet] Benchmark Ultra troubles To: histonet@lists.utsouthwestern.edu Message-ID: 001a01cebf96$45a20a10$d0e61e30$@gmx.at Content-Type: text/plain; charset=us-ascii Hi Ventana-users, we have a special technical problem with our Ultra. It seems, that the liquid in the waste-tube is sometime pressed out of the hole on the platform like a fountain. You can imagine the nice surprise, when the oil drops from the Ultra-roof onto the carousel-top. Everything is oily. The technical service has'nt found a cause until now. The filter in the tube has been changed. Has anyone seen a similar problem? Solved the problem? Gudrun Lang -- Message: 3 Date: Wed, 2 Oct 2013 13:47:22 -0400 From: Kim Donadio one_angel_sec...@yahoo.com Subject: Re: [Histonet] RE: MOHS IPs To: Bauer, Karen L. bauer.ka...@mayo.edu Cc: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Message-ID: 628c0e60-8664-4dbc-b558-f3b5d9fea...@yahoo.com Content-Type: text/plain; charset=us-ascii What detection kit are you using? Sent from my iPhone On Oct 2, 2013, at 10:47 AM, Bauer, Karen L. bauer.ka...@mayo.edu wrote: Thanks for the reply, We are pre-fixing in a formalin substitute, but we'll give the acetone a try. I have diluted the protease, but have not gotten any good results. We'll keep trying with that as well. We've tried multiple incubation times for the cytokeratin... From 5 minutes all the way up to 30 minutes... With no luck. We are using an enhanced polymer DAB. Thanks for the suggestions! I greatly appreciate it! :) Karen Karen L. Bauer HTL/HT (ASCP) | Histology Supervisor | Pathology | MOHS Lab Supervisor |
[Histonet] HistoDeck questions
HistoDeck questions are companion of the Carson' s book. Carson fixes in 37-40% formaldehyde for OilRed O. Any acetone or alcohol will dissolve the fat and OilRedO will be negative or suboptimum. The answer should be A Mesru ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Dacie Iron Stain
Hi Everyone, this is my first time posting, hope this works. One of our pathologists is interested in the Dacie iron stain for bone marrow specimens. Where can I purchase this stain or is this simply a method? I greatly appreciate your help! Melissa Anatomic Pathology Supervisor Nemours Children's Hospital___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Bodian
Hi Helene Have you tried a Bielschowsky instead. It is widely reported to provide better staining than the Bodian. http://www.ncbi.nlm.nih.gov/pubmed/2422580 regards Tony Tony Reilly B.App.Sc. , M.Sc. Chief Scientist, Anatomical Pathology Pathology Queensland-PA Laboratory Health Services Support Agency | Department of Health Level 1, Building 15,Princess Alexandra Hospital Ipswich Road,WOOLLOONGABBA Qld4102 Ph: 07 3176 2412 Mob: 0402 139411 Fax: 07 3176 2930 Email: tony_rei...@health.qld.gov.au Web: www.health.qld.gov.au/qhcss/ Helene Degan deg...@upstate.edu 10/4/2013 6:38 am Hi Thanks to every one that sent me Acid Phosphotase procedure for muscle biopsies. I also need some help with the bodian stain, we are not getting any staining on the slides, we have a procedure that they used years ago and now we can't get it working (we did purchase new chemicals) and we also tried a stain kit with very little results. Thanks again Helene Upstate Medical University ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This email, including any attachments sent with it, is confidential and for the sole use of the intended recipient(s). This confidentiality is not waived or lost, if you receive it and you are not the intended recipient(s), or if it is transmitted/received in error. Any unauthorised use, alteration, disclosure, distribution or review of this email is strictly prohibited. The information contained in this email, including any attachment sent with it, may be subject to a statutory duty of confidentiality if it relates to health service matters. If you are not the intended recipient(s), or if you have received this email in error, you are asked to immediately notify the sender by telephone collect on Australia +61 1800 198 175 or by return email. You should also delete this email, and any copies, from your computer system network and destroy any hard copies produced. If not an intended recipient of this email, you must not copy, distribute or take any action(s) that relies on it; any form of disclosure, modification, distribution and/or publication of this email is also prohibited. Although Queensland Health takes all reasonable steps to ensure this email does not contain malicious software, Queensland Health does not accept responsibility for the consequences if any person's computer inadvertently suffers any disruption to services, loss of information, harm or is infected with a virus, other malicious computer programme or code that may occur as a consequence of receiving this email. Unless stated otherwise, this email represents only the views of the sender and not the views of the Queensland Government. ** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Microtomes
Hi there We recently purchased a Leica microtome and all seems to be going very well. It cuts beautifully. Just warn students not to clean the machine with xylene as ours did and smudged the writing on the microtome. Good luck Subash Govender Anatomical Pathology Research Lab University of Cape Town Medical School South Africa Email: subash.goven...@uct.ac.za UNIVERSITY OF CAPE TOWN This e-mail is subject to the UCT ICT policies and e-mail disclaimer published on our website at http://www.uct.ac.za/about/policies/emaildisclaimer/ or obtainable from +27 21 650 9111. This e-mail is intended only for the person(s) to whom it is addressed. If the e-mail has reached you in error, please notify the author. If you are not the intended recipient of the e-mail you may not use, disclose, copy, redirect or print the content. If this e-mail is not related to the business of UCT it is sent by the sender in the sender's individual capacity. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
