Re: [Histonet] Xylene Substitute Protocols
We have found that Permount works best with the aliphatic hydrocarbons. Sent from my iPad On Jul 24, 2014, at 10:01 AM, Hilliard, Dawud Deshawn dawud_hilli...@med.unc.edu wrote: Hello all, Would anyone mind sharing their xylene substitute protocols for their automatic staining, to include the mountant type for coverslipping? We have tried a few, but without much success. We are currently trying Leica's Sub-X, also without much success. I greatly appreciate the insight! Dawud Hilliard MPM, HTL (ASCP) QIHC Facility Director Animal Histopathology Core Lab Lineberger Comprehensive Cancer Center University of North Carolina - Chapel Hill Chapel Hill, North Carolina 27599 (919) 966-3653 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Automated stainer for HE and Pap
We use the Leica ST5020 here in our cytology lab. We have 3 staining setups. One Pap, one Nons/FNA, one rush Nons/FNA. And the Nons/FNA and rush Nons/FNA share some dishes. It would not be possible to add an HE to this machine. And as previously mentioned, the GYN and Nons/FNA have to be separate. A lot of work to change out dishes and increased risk of error. __ Lynn M. O'Donnell, CT (ASCP), MHA Technical Specialist, Cytology Danbury Hospital 203-739-6704 Email: lynn.o'donn...@wchn.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Brandi Sent: Thursday, July 24, 2014 15:21 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Automated stainer for HE and Pap I am shopping for a new automated stainer and would prefer one that I can do my HE stain as well as my PAP (GY AND NON-GY) stains on. I know that I'm asking for a lot and will have to purchase extra containers to switch back and forth. I'm most interested in the Leica 5020 Gemini. I would greatly appreciate any feedback on these two stainers and if anyone else is utilizing either stainer for both stains. Thanks, Brandi Farris ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Coverslip
Hey everyone, I was wondering if anyone else has seen this occur in their labs and how to resolve this matter. It has to do with the coverslip using the resin tape. However more recently, I have been noticing that there is a drying artifact that happens right as the xylene drys. It looks almost like when water freezes and gives that frosted look. Thanks, Tommy This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Coverslip
We have found this to be a culmination of a couple of things: Bad lot of tape from vendor Drying slides before cover slipping Too much xylene drip during coverslip (dilutes the glue stuff on the tape allowing for weak adhesive) We use Sakura cover slipping tape for more consistent quality and adjust xylene drip accordingly and make sure slides do not sit out very long at all as xylene from the stainer will evaporate (drying slides) rather quickly. Hope this helps. Michael Ann Jones, HT (ASCP) Histology Manager Metropath 7444 W. Alaska Dr. #250 Lakewood, CO 80226 303.634.2511 mjo...@metropath.com On 7/25/14, 7:47 AM, Tommy Mai t...@phenopath.com wrote: Hey everyone, I was wondering if anyone else has seen this occur in their labs and how to resolve this matter. It has to do with the coverslip using the resin tape. However more recently, I have been noticing that there is a drying artifact that happens right as the xylene drys. It looks almost like when water freezes and gives that frosted look. Thanks, Tommy This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Xylene Substitute Protocols
Statlab XS-3 Acrymount. I do H E staining only. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Hilliard, Dawud Deshawn Sent: Thursday, July 24, 2014 9:50 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Xylene Substitute Protocols Hello all, Would anyone mind sharing their xylene substitute protocols for their automatic staining, to include the mountant type for coverslipping? We have tried a few, but without much success. We are currently trying Leica's Sub-X, also without much success. I greatly appreciate the insight! Dawud Hilliard MPM, HTL (ASCP) QIHC Facility Director Animal Histopathology Core Lab Lineberger Comprehensive Cancer Center University of North Carolina - Chapel Hill Chapel Hill, North Carolina 27599 (919) 966-3653 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Calponin
Hi All, I was wondering what stain protocols are being used for Calponin to minimize cross-reactivity with myofibroblasts? We are using a Dako concentrate antibody. I recently re-optimized the stain using a 1:100 dilution and changed the pre-treatment from low pH HIER, to Protease only. This helped a lot with cross-reactivity with myofibroblasts that we were seeing, however we are still seeing some non-specific staining from time to time. Any information would be greatly appreciated! Thanks, Brandy Burnett Cape Cod Hospital ~~ This email and any files transmitted with it are confidential, and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error contact the Help Desk for Cape Cod Healthcare. helpd...@capecodhealth.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] possible TB case
Hey histonetters!! We just performed a frozen section on a lymph node that looks like it may have TB- I know we will have to decontaminate the cryostat but what else do I have to decontaminate? Will I have to do anything special to the processor or microtome? Thank you in advance Fawn - This electronic message may contain information that is confidential or legally privileged. It is intended only for the use of the individual(s) and entity named as recipients in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from any computer. Do not deliver, distribute, or copy this message, and do not disclose its contents or take any action in reliance on the information it contains. Thank you ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Automated stainer for HE and Pap
Contact Sakura René J. On Thursday, July 24, 2014 3:21 PM, Brandi brandihi...@hotmail.com wrote: I am shopping for a new automated stainer and would prefer one that I can do my HE stain as well as my PAP (GY AND NON-GY) stains on. I know that I'm asking for a lot and will have to purchase extra containers to switch back and forth. I'm most interested in the Leica 5020 Gemini. I would greatly appreciate any feedback on these two stainers and if anyone else is utilizing either stainer for both stains. Thanks, Brandi Farris ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Coverslip
Usually these odd results have 2 origins that you should look into: 1- using a different (cheaper and harder) plastic than the originally recommended; and 2- a regulation problem in the amount of xylene dropped into the film René J. On Friday, July 25, 2014 9:47 AM, Tommy Mai t...@phenopath.com wrote: Hey everyone, I was wondering if anyone else has seen this occur in their labs and how to resolve this matter. It has to do with the coverslip using the resin tape. However more recently, I have been noticing that there is a drying artifact that happens right as the xylene drys. It looks almost like when water freezes and gives that frosted look. Thanks, Tommy This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] multitissue control block
Hello, Could someone provide some information on the multi-tissue control block used for IHC? Does anyone have one that is also used for ER/PR too. Thank you Debra Ortiz Technical Director, AP ACL Laboratories, Il debra.or...@advocatehealth.com This e-mail, and any attachments thereto, is intended only for use by the addressee(s) named herein and may contain legally privileged and/or confidential information. If you are not the intended recipient of this e-mail (or the person responsible for delivering this document to the intended recipient), you are hereby notified that any dissemination, distribution, printing or copying of this e-mail, and any attachments thereto, is strictly prohibited. If you have received this e-mail in error, please respond to the individual sending the message and permanently delete the original and any copy of any e-mail and any printout thereof. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] IHC Validation:
Does anyone know the preferred method for IHC validation? Is making a tissue microarray of different known cases the preferred method? Does anyone have experience validating like this? Please let me know if anyone has information/tips to doing so. Thank you. Sent from my iPhone ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Slide Mate Print Quality
In answer to the question about the Slide Mate printing coming off of the slides, we had the same issue about a year ago. At first Thermo though it was the ribbon but it turned out the problem was with the slides. We use the Shandon Superfrost Plus (Cat# 6776214). We contacted Thermo/Shandon and they sent us a different lot. We had to go through about three different lots before the problem was resolved. Beth Ann O'Neil, MT(ASCP)SC, HTL, QIHC one...@wvuhealthcare.commailto:one...@wvuhealthcare.com Histology Supervisor, Technical Specialist Lab: 304 - 293 - 6014 Office: 304 - 293 - 7629 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: multitissue control block
What is it that you want to know? I used to make frozen multiple tissue control blocks for ER/PR many years ago before these antibodies could be used for FFPE tissues. You could do the same thing with paraffin embedded tissue. I've made multi-tissue blocks using a skin biopsy punch tool to obtain small amounts of paraffin-embedded tissues to be used in the control block. Barbara S. Tibbs Histology Supervisor Accurate Diagnostic Labs South Plainfield, NJ barbara.ti...@accuratediagnosticlabs.com 732-839-3374 Cell: 610-809-6508 From: histonet-boun...@lists.utsouthwestern.edu histonet-boun...@lists.utsouthwestern.edu on behalf of Ortiz, Debra debra.or...@advocatehealth.com Sent: Friday, July 25, 2014 3:56 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] multitissue control block Hello, Could someone provide some information on the multi-tissue control block used for IHC? Does anyone have one that is also used for ER/PR too. Thank you Debra Ortiz Technical Director, AP ACL Laboratories, Il debra.or...@advocatehealth.com This e-mail, and any attachments thereto, is intended only for use by the addressee(s) named herein and may contain legally privileged and/or confidential information. If you are not the intended recipient of this e-mail (or the person responsible for delivering this document to the intended recipient), you are hereby notified that any dissemination, distribution, printing or copying of this e-mail, and any attachments thereto, is strictly prohibited. If you have received this e-mail in error, please respond to the individual sending the message and permanently delete the original and any copy of any e-mail and any printout thereof. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: multitissue control block
We used to use a pc. Of proliferative endometrium, a pc of secretory emdometrium and a pc of cervix in a multitissue block for our ER/PgR control. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Barbara Tibbs Sent: Friday, July 25, 2014 2:37 PM To: Ortiz, Debra; Histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: multitissue control block What is it that you want to know? I used to make frozen multiple tissue control blocks for ER/PR many years ago before these antibodies could be used for FFPE tissues. You could do the same thing with paraffin embedded tissue. I've made multi-tissue blocks using a skin biopsy punch tool to obtain small amounts of paraffin-embedded tissues to be used in the control block. Barbara S. Tibbs Histology Supervisor Accurate Diagnostic Labs South Plainfield, NJ barbara.ti...@accuratediagnosticlabs.com 732-839-3374 Cell: 610-809-6508 From: histonet-boun...@lists.utsouthwestern.edu histonet-boun...@lists.utsouthwestern.edu on behalf of Ortiz, Debra debra.or...@advocatehealth.com Sent: Friday, July 25, 2014 3:56 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] multitissue control block Hello, Could someone provide some information on the multi-tissue control block used for IHC? Does anyone have one that is also used for ER/PR too. Thank you Debra Ortiz Technical Director, AP ACL Laboratories, Il debra.or...@advocatehealth.com This e-mail, and any attachments thereto, is intended only for use by the addressee(s) named herein and may contain legally privileged and/or confidential information. If you are not the intended recipient of this e-mail (or the person responsible for delivering this document to the intended recipient), you are hereby notified that any dissemination, distribution, printing or copying of this e-mail, and any attachments thereto, is strictly prohibited. If you have received this e-mail in error, please respond to the individual sending the message and permanently delete the original and any copy of any e-mail and any printout thereof. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] (no subject)
Hi guys, I need to settle on a coverslip machine, I wonder which is most reliable need 5 peoples at minimum or 10 opinions for the recommendations please Aneesh Dhiman University of Alberta hospital ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
