[Histonet] test
test mail - best wishes to all Gudrun ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] paraffin sectioning-dry tissue?
After macro trimming into our blocks (very gently if friable or dry) we place them on an ice tray to soak for a good while. Each well is filled with a little bit of water - this rehydrates the tissue just enough to get a few good sections off the top. we¹ve had success with this method for most any tissue (the more dry the tissue, the longer the soak). Michael Ann Jones, HT (ASCP) Histology Manager Metropath 7444 W. Alaska Dr. #250 Lakewood, CO 80226 303.634.2511 mjo...@metropath.com On 2/5/15, 4:23 AM, Emily Brown talulahg...@gmail.com wrote: Hello all! I just started sectioning mouse liver in paraffin and the tissue is very dry. I know it's not supposed to have water due to the processing, but the weird thing is that one tech's solution is to put a wet kimwipe on the block for a while. It seems to me that there is a larger processing issue if this is happening, am I correct? And why add water when you've already dehydrated it? Unfortunately, we do not have the set up to embed them ourselves, so we have to send them to a histology lab. They were sectioning for us, but they are backlogged, so my boss wants me to do it. Therefore, I can't tell you how they were processed, but I think usually the histology lab manages to get good sections. Is putting a wet kimwipe (using distilled water) the best way to get rid of chatter that's only in the tissue? The surrounding paraffin sections excellent. This may have been answered already, but a very quick google search didn't help. My googlefu is probably erratic as it's still early. Emily By bitching and bitching and bitching, they could exhaust the drama of their own horror stories. Grow bored. Only then could they accept a new story for their lives. Move forward. -Chuck Palahniuk, Haunted ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] use of denatured alcohol
Hello, Our lab is considering switching to denatured alcohol as a cost saving initiative. Is anyone using denatured alcohols and performing subsequent Class II IHC testing with no impact to results? Thank-you, Joanna Joanna Bartczak MLT II - Immunohistochemistry Calgary Laboratory Services 403-770-3695 This message and any attached documents are only for the use of the intended recipient(s), are confidential and may contain privileged information. Any unauthorized review, use, retransmission, or other disclosure is strictly prohibited. If you have received this message in error, please notify the sender immediately, and then delete the original message. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] C-Kit
Hi All, I have a pathologist that is wanting me to bring in C-kit. My question is am I required by CAP to also do the Survey for predicative markers for C-kit? Thanks for your time and answers. Mike ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Looking for ribbons for old Surgipath VCP cassette printer
Hi everyone - For those of us who are still in the dark ages... have an old Surgipath VCP cassette printer; does anyone know where to get replacement ribbons for these machines these days? Does anyone know who supports these machines anymore? Thanks so much - -Nancy Stedman ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Looking for ribbons for old Surgipath VCP cassette printer
We have one too. We have been getting ribbons from Surgipath/Leica. The number is 38V0590001F. we have Tech One Biomedical service it. They have been great. Lynn M Burton Histology Animal Disease Lab Galesburg, Il 309-344-2451 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Stedman, Nancy Sent: Thursday, February 05, 2015 8:54 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Looking for ribbons for old Surgipath VCP cassette printer Hi everyone - For those of us who are still in the dark ages... have an old Surgipath VCP cassette printer; does anyone know where to get replacement ribbons for these machines these days? Does anyone know who supports these machines anymore? Thanks so much - -Nancy Stedman ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] denatured alcohol
Dear Histonetters: The other day, someone sent me a sample in (allegedly) denaturated alcohol. I asked the colleague to let me know what was the concentration of ethanol in the sample and the answer was about 80%. From the smell, I suspect the rest is methanol. I need to dehydrate the sample through an ethanol series. Could someone recommend where should I get started? OK to begin at 80% or to assume that the sample is dehydrated enough in this denaturated alcohol. Thereafter, I will process the sample via a critical point dryer. If you have any suggestions, please email me directly at blayjo...@gmail.com . Thank you. Sincerely, Jorge Jorge A. Santiago-Blay, PhD blaypublishers.com http://blayjorge.wordpress.com/ http://paleobiology.si.edu/staff/individuals/santiagoblay.cfm ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Troponin is biomarker of heart attacks
Dear Histonnetters: I am not sure the range of expertise of the members may reach this far but let me try. Question: Could sometone tell me (blayjo...@gmail.com) why is blood troponin is biomarker of heart attacks. I am aware that is is not the only biomarker but nowhere have I yet seen an explanation why not tropomyosin of other protein components of the cardiac muscle that may find themselves in the blood stream as a result of damage or death due to the heart attack. If you know of a human physiology / human anatomy listserver, please, let me know. Gratefully, Jorge blayjo...@gmail.com -- Jorge A. Santiago-Blay, PhD blaypublishers.com http://blayjorge.wordpress.com/ http://paleobiology.si.edu/staff/individuals/santiagoblay.cfm ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] denatured alcohol
You will have to start in 80% ethanol and you cannot assume the tissue is dehydrated enough.René J. On Thursday, February 5, 2015 3:57 PM, Jorge A. Santiago-Blay blayjo...@gmail.com wrote: Dear Histonetters: The other day, someone sent me a sample in (allegedly) denaturated alcohol. I asked the colleague to let me know what was the concentration of ethanol in the sample and the answer was about 80%. From the smell, I suspect the rest is methanol. I need to dehydrate the sample through an ethanol series. Could someone recommend where should I get started? OK to begin at 80% or to assume that the sample is dehydrated enough in this denaturated alcohol. Thereafter, I will process the sample via a critical point dryer. If you have any suggestions, please email me directly at blayjo...@gmail.com . Thank you. Sincerely, Jorge Jorge A. Santiago-Blay, PhD blaypublishers.com http://blayjorge.wordpress.com/ http://paleobiology.si.edu/staff/individuals/santiagoblay.cfm ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] paraffin sectioning-dry tissue?
This is common with mouse and rat tissues, they get over-dried with a typical processing schedule. Soaking the face of the block with a kimwipe wet with ice water for 60 -120 seconds will enable you to cut 10 nice sections, maybe more. Geoff On 2/5/2015 6:23 AM, Emily Brown wrote: Hello all! I just started sectioning mouse liver in paraffin and the tissue is very dry. I know it's not supposed to have water due to the processing, but the weird thing is that one tech's solution is to put a wet kimwipe on the block for a while. It seems to me that there is a larger processing issue if this is happening, am I correct? And why add water when you've already dehydrated it? Unfortunately, we do not have the set up to embed them ourselves, so we have to send them to a histology lab. They were sectioning for us, but they are backlogged, so my boss wants me to do it. Therefore, I can't tell you how they were processed, but I think usually the histology lab manages to get good sections. Is putting a wet kimwipe (using distilled water) the best way to get rid of chatter that's only in the tissue? The surrounding paraffin sections excellent. This may have been answered already, but a very quick google search didn't help. My googlefu is probably erratic as it's still early. Emily By bitching and bitching and bitching, they could exhaust the drama of their own horror stories. Grow bored. Only then could they accept a new story for their lives. Move forward. -Chuck Palahniuk, Haunted ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- -- ** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732) 235-4583; fax: -4029 mcaul...@rwjms.rutgers.edu ** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] paraffin sectioning-dry tissue?
Yes, exactly what Mike and Geoff said. All mouse tissue, especially liver, can be really dry and needs a 'soak'. I have left them for an hour before now but don't leave it for longer than 4 hours though because it can start to swell and de-process! You will still only get a few non-chattery sections so be gentle. Thinner sections also help too (3-4.5). Plus low um polishing after you trim Good luck! It is weird at first but you will get used to it! Caroline Sent from my iPhone On Feb 5, 2015, at 6:29 AM, Geoff mcaul...@rwjms.rutgers.edu wrote: This is common with mouse and rat tissues, they get over-dried with a typical processing schedule. Soaking the face of the block with a kimwipe wet with ice water for 60 -120 seconds will enable you to cut 10 nice sections, maybe more. Geoff On 2/5/2015 6:23 AM, Emily Brown wrote: Hello all! I just started sectioning mouse liver in paraffin and the tissue is very dry. I know it's not supposed to have water due to the processing, but the weird thing is that one tech's solution is to put a wet kimwipe on the block for a while. It seems to me that there is a larger processing issue if this is happening, am I correct? And why add water when you've already dehydrated it? Unfortunately, we do not have the set up to embed them ourselves, so we have to send them to a histology lab. They were sectioning for us, but they are backlogged, so my boss wants me to do it. Therefore, I can't tell you how they were processed, but I think usually the histology lab manages to get good sections. Is putting a wet kimwipe (using distilled water) the best way to get rid of chatter that's only in the tissue? The surrounding paraffin sections excellent. This may have been answered already, but a very quick google search didn't help. My googlefu is probably erratic as it's still early. Emily By bitching and bitching and bitching, they could exhaust the drama of their own horror stories. Grow bored. Only then could they accept a new story for their lives. Move forward. -Chuck Palahniuk, Haunted ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- -- ** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732) 235-4583; fax: -4029 mcaul...@rwjms.rutgers.edu ** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] paraffin sectioning-dry tissue?
We use a 5% glycerin in denatured alcohol for our 100% alcohol on our tissue processor for routine animal tissues, this reduces the over dehydration of the animal tissue and greatly reduces the time required to soak the blocks. Warning, if processing fat or cell pellets do not use this, we switch the containers to straight 100% reagent alcohol for them. For fat we have a longer processing schedule and for cell pellets we have a short processing cycle. James Watson HT ASCP GNF Genomics Institute of the Novartis Research Foundation Scientific Technical Leader II, Histology Tel 858-332-4647 Fax 858-812-1915 jwat...@gnf.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Caroline Miller Sent: Thursday, February 05, 2015 6:41 AM To: Geoff Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] paraffin sectioning-dry tissue? Yes, exactly what Mike and Geoff said. All mouse tissue, especially liver, can be really dry and needs a 'soak'. I have left them for an hour before now but don't leave it for longer than 4 hours though because it can start to swell and de-process! You will still only get a few non-chattery sections so be gentle. Thinner sections also help too (3-4.5). Plus low um polishing after you trim Good luck! It is weird at first but you will get used to it! Caroline Sent from my iPhone On Feb 5, 2015, at 6:29 AM, Geoff mcaul...@rwjms.rutgers.edu wrote: This is common with mouse and rat tissues, they get over-dried with a typical processing schedule. Soaking the face of the block with a kimwipe wet with ice water for 60 -120 seconds will enable you to cut 10 nice sections, maybe more. Geoff On 2/5/2015 6:23 AM, Emily Brown wrote: Hello all! I just started sectioning mouse liver in paraffin and the tissue is very dry. I know it's not supposed to have water due to the processing, but the weird thing is that one tech's solution is to put a wet kimwipe on the block for a while. It seems to me that there is a larger processing issue if this is happening, am I correct? And why add water when you've already dehydrated it? Unfortunately, we do not have the set up to embed them ourselves, so we have to send them to a histology lab. They were sectioning for us, but they are backlogged, so my boss wants me to do it. Therefore, I can't tell you how they were processed, but I think usually the histology lab manages to get good sections. Is putting a wet kimwipe (using distilled water) the best way to get rid of chatter that's only in the tissue? The surrounding paraffin sections excellent. This may have been answered already, but a very quick google search didn't help. My googlefu is probably erratic as it's still early. Emily By bitching and bitching and bitching, they could exhaust the drama of their own horror stories. Grow bored. Only then could they accept a new story for their lives. Move forward. -Chuck Palahniuk, Haunted ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- -- ** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732) 235-4583; fax: -4029 mcaul...@rwjms.rutgers.edu ** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: ALCIAN YELLOW ALCIAN BLUE 8GX
Dear Sirs, We are manufacturer of ALCIAN BLUE 8GX and ALCIAN YELLOW. Looking forward to hearing from you asap. Kind Regards Minggeng Wang, Ph.D/President SUZHOU SINOERA CHEM CO., LTD. 125 Binhe Road, Suzhou New Hi-Tech District, 215011 China Tel: 0086 512 68246939 Fax: 0086 512 68258994 Inquiries:sinoerac...@sina.cn General Questions: billions1...@outlook.com http://www.sinoeratech.com http://www.sinoerachem.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] paraffin sectioning-dry tissue?
By adding water, ice, or warm humidity (through exhalations) to the mix, though, doesn't the block contract/expand? Wouldn't that change the ultimate thickness of the section? I've always wondered how much it affects things. Could your sections be 1 um (or multiple um!) thicker/thinner that you expect? What happens if individual blocks contract/expand differently (due to the amount of time left soaking, how far into the block you've cut since you last soaked, etc.)? I feel like you couldn't properly compare quantifications across a study if the thickness of your tissue is an unknown variable. Am I overthinking this? Lucie Guernsey UC San Diego (858) 822-5797 lguern...@ucsd.edu On Thu, Feb 5, 2015 at 7:35 AM, James Watson jwat...@gnf.org wrote: We use a 5% glycerin in denatured alcohol for our 100% alcohol on our tissue processor for routine animal tissues, this reduces the over dehydration of the animal tissue and greatly reduces the time required to soak the blocks. Warning, if processing fat or cell pellets do not use this, we switch the containers to straight 100% reagent alcohol for them. For fat we have a longer processing schedule and for cell pellets we have a short processing cycle. James Watson HT ASCP GNF Genomics Institute of the Novartis Research Foundation Scientific Technical Leader II, Histology Tel858-332-4647 Fax 858-812-1915 jwat...@gnf.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto: histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Caroline Miller Sent: Thursday, February 05, 2015 6:41 AM To: Geoff Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] paraffin sectioning-dry tissue? Yes, exactly what Mike and Geoff said. All mouse tissue, especially liver, can be really dry and needs a 'soak'. I have left them for an hour before now but don't leave it for longer than 4 hours though because it can start to swell and de-process! You will still only get a few non-chattery sections so be gentle. Thinner sections also help too (3-4.5). Plus low um polishing after you trim Good luck! It is weird at first but you will get used to it! Caroline Sent from my iPhone On Feb 5, 2015, at 6:29 AM, Geoff mcaul...@rwjms.rutgers.edu wrote: This is common with mouse and rat tissues, they get over-dried with a typical processing schedule. Soaking the face of the block with a kimwipe wet with ice water for 60 -120 seconds will enable you to cut 10 nice sections, maybe more. Geoff On 2/5/2015 6:23 AM, Emily Brown wrote: Hello all! I just started sectioning mouse liver in paraffin and the tissue is very dry. I know it's not supposed to have water due to the processing, but the weird thing is that one tech's solution is to put a wet kimwipe on the block for a while. It seems to me that there is a larger processing issue if this is happening, am I correct? And why add water when you've already dehydrated it? Unfortunately, we do not have the set up to embed them ourselves, so we have to send them to a histology lab. They were sectioning for us, but they are backlogged, so my boss wants me to do it. Therefore, I can't tell you how they were processed, but I think usually the histology lab manages to get good sections. Is putting a wet kimwipe (using distilled water) the best way to get rid of chatter that's only in the tissue? The surrounding paraffin sections excellent. This may have been answered already, but a very quick google search didn't help. My googlefu is probably erratic as it's still early. Emily By bitching and bitching and bitching, they could exhaust the drama of their own horror stories. Grow bored. Only then could they accept a new story for their lives. Move forward. -Chuck Palahniuk, Haunted ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- -- ** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732) 235-4583; fax: -4029 mcaul...@rwjms.rutgers.edu ** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu
[Histonet] Leica ASP basket transport
Our lab has standardized all cassette racking into the Leica ASP 300 basket. For locations that ship back to the main lab I have not found a suitable hard plastic storage container that fits this basket. I performed an in-person search at home depot and lowes without success. 14047643576 Basket set Leica ASP300 Shttp://www.leicabiosystems.com/ Looking to see what others are using for transport of this particular style of basket. I am struggling to find plastic storage container that will stack 3 high on the hook. Each basket is approx. 8x13x3 inches. Thanks, Nick Nick Ingrao, MBA, MT(ASCP) Manager, Anatomic Pathology Kaleida Health: Buffalo General Medical Center 100 High St Buffalo, NY 14203 Office:(716) 859-2028 Pager: (716) 642-0232 Fax: (716) 859-2393 The Keeping You Informed section of Kaleida Health`s website features a wealth of information, stories and pictures about our valued workforce and the tremendous momentum our organization is experiencing. Check us out at: www.kaleidahealth.org/kyi CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. If you are unable to contact the sender or you are not sure as to whether you are the intended recipient, please call Kaleida HealthÂs Technology Assistance Center at (716) 859-. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Leica ASP basket transport
Good luck probably some type of plastic ware that u can find at wallmart Sent from my Verizon Wireless 4G LTE smartphone Original message From: Ingrao, Nicholas ning...@kaleidahealth.org Date:02/05/2015 11:19 AM (GMT-05:00) To: 'histonet@lists.utsouthwestern.edu' histonet@lists.utsouthwestern.edu Subject: [Histonet] Leica ASP basket transport Our lab has standardized all cassette racking into the Leica ASP 300 basket. For locations that ship back to the main lab I have not found a suitable hard plastic storage container that fits this basket. I performed an in-person search at home depot and lowes without success. 14047643576 Basket set Leica ASP300 Shttp://www.leicabiosystems.com/ Looking to see what others are using for transport of this particular style of basket. I am struggling to find plastic storage container that will stack 3 high on the hook. Each basket is approx. 8x13x3 inches. Thanks, Nick Nick Ingrao, MBA, MT(ASCP) Manager, Anatomic Pathology Kaleida Health: Buffalo General Medical Center 100 High St Buffalo, NY 14203 Office:(716) 859-2028 Pager: (716) 642-0232 Fax: (716) 859-2393 The Keeping You Informed section of Kaleida Health`s website features a wealth of information, stories and pictures about our valued workforce and the tremendous momentum our organization is experiencing. Check us out at: www.kaleidahealth.org/kyi CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. If you are unable to contact the sender or you are not sure as to whether you are the intended recipient, please call Kaleida Health��s Technology Assistance Center at (716) 859-. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Leica ASP basket transport
Glad to know I'm not the only one who haunts the plasticware sections of stores looking for the ideal container for the lab! We use some Rubbermaid containers for the Peloris and VIP baskets, but they are not fully 3 high. I suggest looking at Bed Bath and Beyond, Container Store, all supermarkets, etc. I looked for many months for the ideal container to hold our EM embedding resins bottles and finally found it at the Container Store. Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ingrao, Nicholas Sent: Thursday, February 05, 2015 8:19 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Leica ASP basket transport Our lab has standardized all cassette racking into the Leica ASP 300 basket. For locations that ship back to the main lab I have not found a suitable hard plastic storage container that fits this basket. I performed an in-person search at home depot and lowes without success. 14047643576 Basket set Leica ASP300 Shttp://www.leicabiosystems.com/ Looking to see what others are using for transport of this particular style of basket. I am struggling to find plastic storage container that will stack 3 high on the hook. Each basket is approx. 8x13x3 inches. Thanks, Nick Nick Ingrao, MBA, MT(ASCP) Manager, Anatomic Pathology Kaleida Health: Buffalo General Medical Center 100 High St Buffalo, NY 14203 Office:(716) 859-2028 Pager: (716) 642-0232 Fax: (716) 859-2393 The Keeping You Informed section of Kaleida Health`s website features a wealth of information, stories and pictures about our valued workforce and the tremendous momentum our organization is experiencing. Check us out at: www.kaleidahealth.org/kyi CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. If you are unable to contact the sender or you are not sure as to whether you are the intended recipient, please call Kaleida Health??s Technology Assistance Center at (716) 859-. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Troponin is biomarker of heart attacks
troponin I is the only troponin assay to show cardiac muscle injuty. -Original Message- From: Jorge A. Santiago-Blay blayjo...@gmail.com To: Histonet Histonet@lists.utsouthwestern.edu Sent: Thu, Feb 5, 2015 3:02 pm Subject: [Histonet] Troponin is biomarker of heart attacks Dear Histonnetters: I am not sure the range of expertise of the members may reach this far but let me try. Question: Could sometone tell me (blayjo...@gmail.com) why is blood troponin is biomarker of heart attacks. I am aware that is is not the only biomarker but nowhere have I yet seen an explanation why not tropomyosin of other protein components of the cardiac muscle that may find themselves in the blood stream as a result of damage or death due to the heart attack. If you know of a human physiology / human anatomy listserver, please, let me know. Gratefully, Jorge blayjo...@gmail.com -- Jorge A. Santiago-Blay, PhD blaypublishers.com http://blayjorge.wordpress.com/ http://paleobiology.si.edu/staff/individuals/santiagoblay.cfm ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Troponin is biomarker of heart attacks
Hi Jorge - If I understand your question correctly - it is because cardiac troponin is specific for cardiac muscle damage. Other muscle enzymes/proteins we can assay for could also be elevated with skeletal muscle damage. -Nancy Stedman From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of Jorge A. Santiago-Blay [blayjo...@gmail.com] Sent: Thursday, February 05, 2015 4:01 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Troponin is biomarker of heart attacks Dear Histonnetters: I am not sure the range of expertise of the members may reach this far but let me try. Question: Could sometone tell me (blayjo...@gmail.com) why is blood troponin is biomarker of heart attacks. I am aware that is is not the only biomarker but nowhere have I yet seen an explanation why not tropomyosin of other protein components of the cardiac muscle that may find themselves in the blood stream as a result of damage or death due to the heart attack. If you know of a human physiology / human anatomy listserver, please, let me know. Gratefully, Jorge blayjo...@gmail.com -- Jorge A. Santiago-Blay, PhD blaypublishers.com http://blayjorge.wordpress.com/ http://paleobiology.si.edu/staff/individuals/santiagoblay.cfm ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Substitute for Safran du Gatinais
I am doing the Movats Pentachrome stain which requires the use of Safran du Gatinais. I would like to know if there is any substitute for Safran du Gatinais. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] paraffin sectioning-dry tissue? - long response - image analysis related
Lucie You are not overthinking this at all if you are utilizing your sections for any image analysis applications. You need to be able to standardize as much of the histology process as possible. There are so many other parameters that can cause section thickness to fluctuate. Such as the sharpness of your knife, how fast you turn the hand wheel. Blowing on the block is not acceptable in our lab that will create thicker sections since you are warming up the block. Great care is taken to standardize what we do, from soaking blocks to how we collect the sections to placement on the slide, and how often we move our knife blade. We routinely soak all of our blocks but we keep in mind so many other factors when we are providing histology for image analysis. It starts at the beginning with fixation, it all has to be standardized, our goal is to decrease the potential for variability. That is on our minds at every step through the histology process. The other thing to consider is how well the algorithm functions - you need to determine the limits of the algorithm and when it will stop functioning properly, which is usually due to staining issues (over or under staining or inconsistent staining), section thickness and overall section and stain quality which is so important. As a part of algorithm validation we test for these parameters we want to understand where we lose functionality, accuracy and precision of the algorithm. So we look at different section thicknesses and how that impacts analysis, we look at over and under staining parameters to see how that affects the algorithm, etc. Just my two cents. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell l...@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lucie Guernsey Sent: Thursday, February 05, 2015 10:22 AM To: James Watson Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] paraffin sectioning-dry tissue? By adding water, ice, or warm humidity (through exhalations) to the mix, though, doesn't the block contract/expand? Wouldn't that change the ultimate thickness of the section? I've always wondered how much it affects things. Could your sections be 1 um (or multiple um!) thicker/thinner that you expect? What happens if individual blocks contract/expand differently (due to the amount of time left soaking, how far into the block you've cut since you last soaked, etc.)? I feel like you couldn't properly compare quantifications across a study if the thickness of your tissue is an unknown variable. Am I overthinking this? Lucie Guernsey UC San Diego (858) 822-5797 lguern...@ucsd.edu On Thu, Feb 5, 2015 at 7:35 AM, James Watson jwat...@gnf.org wrote: We use a 5% glycerin in denatured alcohol for our 100% alcohol on our tissue processor for routine animal tissues, this reduces the over dehydration of the animal tissue and greatly reduces the time required to soak the blocks. Warning, if processing fat or cell pellets do not use this, we switch the containers to straight 100% reagent alcohol for them. For fat we have a longer processing schedule and for cell pellets we have a short processing cycle. James Watson HT ASCP GNF Genomics Institute of the Novartis Research Foundation Scientific Technical Leader II, Histology Tel858-332-4647 Fax 858-812-1915 jwat...@gnf.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto: histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Caroline Miller Sent: Thursday, February 05, 2015 6:41 AM To: Geoff Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] paraffin sectioning-dry tissue? Yes, exactly what Mike and Geoff said. All mouse tissue, especially liver, can be really dry and needs a 'soak'. I have left them for an hour before now but don't leave it for longer than 4 hours though because it can start to swell and de-process! You will still only get a few non-chattery sections so be gentle. Thinner sections also help too (3-4.5). Plus low um polishing after you trim Good luck! It is weird at first but you will get used to it! Caroline Sent from my iPhone On Feb 5, 2015, at 6:29 AM, Geoff mcaul...@rwjms.rutgers.edu wrote: This is common with mouse and rat tissues, they get over-dried with a typical processing schedule. Soaking the face of the block with a kimwipe wet with ice water for 60 -120 seconds will enable you to cut 10 nice sections, maybe more. Geoff On 2/5/2015 6:23 AM, Emily Brown wrote: Hello all! I just started sectioning mouse liver in paraffin and
RE: [Histonet] Cryosectioning undecalcified bone
Hello, I believe that the products for the CryoJane tape transfer are still available from Fisher. Helen L. Fedor Oncology Tissue Services, Manager Johns Hopkins University 411 N. Caroline St Room 310 Basement| Bond St Annex Building Baltimore, MD | 21231 410-614-1660 http://tmalab.jhmi.edu/ -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Elizabeth Chlipala Sent: Thursday, February 05, 2015 1:08 PM To: Orla M Gallagher; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Cryosectioning undecalcified bone Orla There is an article in the Journal of Histotechnology from a while ago from John Tarpley that addressed methods for this. I have a pdf of it and I will send in another e-mail. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell l...@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Orla M Gallagher Sent: Thursday, February 05, 2015 10:56 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cryosectioning undecalcified bone Dear Histonetters, We would like to develop cryosectioning for undecalcified mouse and rat bones. Previous attempts to use the CryoJane system from Instrumedics with an old Bright cryostat and solid tungsten carbide blade a few years ago didn't result in successful reproducible sections - marrow without bone or bone without marrow on the slide, in spite of various freezing and prep. protocols used by my colleague. I suspect the old cryostat and blade weren't helping either. Have you any recommendations on the best cryostat to use to do this? We'd like to also use the cryostat for standard soft tissue sectioning. I've seen Leica mentioned in a few papers relating to CryoJane. There appears also to be a tape transfer method from Kawamoto's Section-Lab Co. Ltd. (i...@section-lab.jp) Does anyone use this method or know whether the consumables are still available for purchase, as the website seems dormant? Thanks for any advice, Orla -- ** Ms. Orla Gallagher Bone Analysis Laboratory Mellanby Centre for Bone Research Department of Human Metabolism D Floor Medical School University of Sheffield Beech Hill Road Sheffield S10 2RX UK Website: http://mellanbycentre.dept.shef.ac.uk Tel: 0044114-2713337 (office) 0044114-2713174 (lab) E-Mail:o.m.gallag...@sheffield.ac.uk *STOP*: Do you really need to print this e-mail? *BE GREEN:* Keep it on the screen. http://www.shef.ac.uk/faculty/medicine-dentistry-health/2.9274/emailwording http://www.sheffield.ac.uk/humanmetabolism/greenimpact http://www.shef.ac.uk/faculty/medicine-dentistry-health/2.9274/emailwording http://www.sheffield.ac.uk/visitors/mapsandtravel ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Cryosectioning undecalcified bone
Dear Histonetters, We would like to develop cryosectioning for undecalcified mouse and rat bones. Previous attempts to use the CryoJane system from Instrumedics with an old Bright cryostat and solid tungsten carbide blade a few years ago didn't result in successful reproducible sections - marrow without bone or bone without marrow on the slide, in spite of various freezing and prep. protocols used by my colleague. I suspect the old cryostat and blade weren't helping either. Have you any recommendations on the best cryostat to use to do this? We'd like to also use the cryostat for standard soft tissue sectioning. I've seen Leica mentioned in a few papers relating to CryoJane. There appears also to be a tape transfer method from Kawamoto's Section-Lab Co. Ltd. (i...@section-lab.jp) Does anyone use this method or know whether the consumables are still available for purchase, as the website seems dormant? Thanks for any advice, Orla -- ** Ms. Orla Gallagher Bone Analysis Laboratory Mellanby Centre for Bone Research Department of Human Metabolism D Floor Medical School University of Sheffield Beech Hill Road Sheffield S10 2RX UK Website: http://mellanbycentre.dept.shef.ac.uk Tel: 0044114-2713337 (office) 0044114-2713174 (lab) E-Mail:o.m.gallag...@sheffield.ac.uk *STOP*: Do you really need to print this e-mail? *BE GREEN:* Keep it on the screen. http://www.shef.ac.uk/faculty/medicine-dentistry-health/2.9274/emailwording http://www.sheffield.ac.uk/humanmetabolism/greenimpact http://www.shef.ac.uk/faculty/medicine-dentistry-health/2.9274/emailwording http://www.sheffield.ac.uk/visitors/mapsandtravel ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Cryosectioning undecalcified bone
Orla There is an article in the Journal of Histotechnology from a while ago from John Tarpley that addressed methods for this. I have a pdf of it and I will send in another e-mail. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell l...@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Orla M Gallagher Sent: Thursday, February 05, 2015 10:56 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cryosectioning undecalcified bone Dear Histonetters, We would like to develop cryosectioning for undecalcified mouse and rat bones. Previous attempts to use the CryoJane system from Instrumedics with an old Bright cryostat and solid tungsten carbide blade a few years ago didn't result in successful reproducible sections - marrow without bone or bone without marrow on the slide, in spite of various freezing and prep. protocols used by my colleague. I suspect the old cryostat and blade weren't helping either. Have you any recommendations on the best cryostat to use to do this? We'd like to also use the cryostat for standard soft tissue sectioning. I've seen Leica mentioned in a few papers relating to CryoJane. There appears also to be a tape transfer method from Kawamoto's Section-Lab Co. Ltd. (i...@section-lab.jp) Does anyone use this method or know whether the consumables are still available for purchase, as the website seems dormant? Thanks for any advice, Orla -- ** Ms. Orla Gallagher Bone Analysis Laboratory Mellanby Centre for Bone Research Department of Human Metabolism D Floor Medical School University of Sheffield Beech Hill Road Sheffield S10 2RX UK Website: http://mellanbycentre.dept.shef.ac.uk Tel: 0044114-2713337 (office) 0044114-2713174 (lab) E-Mail:o.m.gallag...@sheffield.ac.uk *STOP*: Do you really need to print this e-mail? *BE GREEN:* Keep it on the screen. http://www.shef.ac.uk/faculty/medicine-dentistry-health/2.9274/emailwording http://www.sheffield.ac.uk/humanmetabolism/greenimpact http://www.shef.ac.uk/faculty/medicine-dentistry-health/2.9274/emailwording http://www.sheffield.ac.uk/visitors/mapsandtravel ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Leica ASP basket transport
Not sure what your baskets look like, but we found Rubbermaid-like travel shoe boxes at Walmart that work really well for us. They're deeper than what we found in the kitchen department. Look by the luggage and travel stuff. (We also haunt the plasticware sections of stores... :D) Laura Jones B.A., HT, PBT (ASCP) | Lead Tech, Histology | Community Health Systems 740 East State Street | Sharon PA | Phone (724)983-3950 | Fax (724)983-3982 www.sharonregional.com | lpjo...@srhs-pa.org From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy [timothy.mor...@ucsf.edu] Sent: Thursday, February 05, 2015 11:45 AM To: 'Ingrao, Nicholas'; 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] RE: Leica ASP basket transport Glad to know I'm not the only one who haunts the plasticware sections of stores looking for the ideal container for the lab! We use some Rubbermaid containers for the Peloris and VIP baskets, but they are not fully 3 high. I suggest looking at Bed Bath and Beyond, Container Store, all supermarkets, etc. I looked for many months for the ideal container to hold our EM embedding resins bottles and finally found it at the Container Store. Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ingrao, Nicholas Sent: Thursday, February 05, 2015 8:19 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Leica ASP basket transport Our lab has standardized all cassette racking into the Leica ASP 300 basket. For locations that ship back to the main lab I have not found a suitable hard plastic storage container that fits this basket. I performed an in-person search at home depot and lowes without success. 14047643576 Basket set Leica ASP300 Shttp://www.leicabiosystems.com/ Looking to see what others are using for transport of this particular style of basket. I am struggling to find plastic storage container that will stack 3 high on the hook. Each basket is approx. 8x13x3 inches. Thanks, Nick Nick Ingrao, MBA, MT(ASCP) Manager, Anatomic Pathology Kaleida Health: Buffalo General Medical Center 100 High St Buffalo, NY 14203 Office:(716) 859-2028 Pager: (716) 642-0232 Fax: (716) 859-2393 The Keeping You Informed section of Kaleida Health`s website features a wealth of information, stories and pictures about our valued workforce and the tremendous momentum our organization is experiencing. Check us out at: www.kaleidahealth.org/kyi CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. If you are unable to contact the sender or you are not sure as to whether you are the intended recipient, please call Kaleida Health??s Technology Assistance Center at (716) 859-. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains.
[Histonet] Job opportunity: Research Histology Technician in Bastrop, TX
Job opportunity for a research histology technician at MD Anderson Cancer Center at the BASTROP, TX location. (approximately 35 miles from Austin, TX) http://jobs.mdanderson.org/texas/research/jobid6803661-research-histology-technician-bastrop-texas-jobs Beth K. Chaffee, DVM, PhD, DACVP Assistant Professor Michale E. Keeling Center for Comparative Medicine and Research MD Anderson Cancer Center 650 Cool Water Dr. Bastrop, TX 78602 Tel. (512) 321-3991 Fax (512) 332-5397 bkchaf...@mdanderson.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Recall: Rick
Scott, Catherine L would like to recall the message, Rick. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Rick
Rick had a situation at home and had to leave about noon ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] TRAP staining FFPE sections
Hi all, I just performed TRAP staining on ffpe edta delcaled bone sections using the Sigma kit (#387A), and was wondering if anyone had experience with reducing the yellow staining over the entire tissue due to the Fast Garnet GBC. I had positive TRAP staining that is red, but there is a lack of contrast due to the yellow that I'd like to improve. Thanks! -- Melanie Smith, MS melsm...@udel.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
