[Histonet] Decalcification using EDTA
Hello Histonetters Is it imperative OR optional to maintain samples at below 4 degrees celcius when using EDTA to decalcify bone samples Thank You ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Nuclear Artifact
This seems like a classic case of drying of biopsies prior to fixation. This can occur if biopsies are placed on absorbent paper (or on disinfecting alcohol swabs, heaven forbid). From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of Sue [suetp...@comcast.net] Sent: Friday, 24 April 2015 10:51 AM To: Lisa Roy Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Nuclear Artifact Hi All So I have been seeing the same issue as I stated in past e-mails. I did one test and fixed a colon biopsy in formaldehyde and left it in overnight and processed next day. I was hoping that I could reproduce the artifact. The tissue was beautiful. At my pathologists requests we changed the paraffin temperature tonight is the first night. I do not think this i the issue. We are going to transfer our biopsies to another tissue processor just for test. I brought up in the past that i think the issue starts prior to the histo lab, my pathologist tended to disagree, but I think he is chaining his mind since my one common detonator is a PA. I do not think that she wets her blue wrap paper enough and the tissue sits on the paper dry she also fold the paper so tight that it is possible for the small biopsies to get stuck in a fold. My pathologists actually came in and said he thought I may be right. Wow. That is my next test. We are requiring our staff to do so much work that they tend to rush and as I have stated in the past grossing sets the tone for every step the nistotech is responsible for and if it is not prepared correctly in the gross lab the histologist cannot fix it. An old adage we are not magicians. Sue Paturzo TJUH ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet * This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. * ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE:Histology in higher education
Thanks for the info. I just signed up. The whole program sounds like a great idea. A few years ago I spoke at my son's middle school about histology and what the job entails. It is challenging to figure out how to reach kids at their level. I may talk about the program in my class next fall to introduce them to outreach in the science fields. Great info. Sincerely, Toysha N. Mayer, D.H.Sc., MBA, HT (ASCP) Instructor/Education Coordinator Program in Histotechnology School of Health Professions UT M.D. Anderson Cancer Center 713.563-3481 Message: 11 Date: Wed, 22 Apr 2015 16:24:28 + From: Piche, Jessica jpi...@wtbyhosp.org Subject: RE: [Histonet] histology in higher education To: 'Grantham, Andrea L - (algranth)' algra...@email.arizona.edu, \\ histonet@lists.utsouthwestern.edu Message-ID: 631955447a364b45b9458d2905635110d8bf6...@win08-mbx-01.wtbyhosp.org Content-Type: text/plain; charset=us-ascii Thank you for sharing this information Andi. I'd like to do something like this and I'm going to send this on to my daughters science teachers at school. I think it's a great idea. It always amazed me all the different jobs in hospitals alone that are available for kids and adults alike and no one knows they exist. Especially histology. Looking forward to passing this on! Thanks again, Jessica Piche, HT(ASCP) Waterbury Hospital CT -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Grantham, Andrea L - (algranth) Sent: Wednesday, April 22, 2015 12:01 PM To: Subject: RE: [Histonet] histology in higher education Bonnie, and anybody who wants to do this: www.prescientist.org From: Whitaker, Bonnie [bonnie.whita...@osumc.edu] Sent: Wednesday, April 22, 2015 8:45 AM To: Grantham, Andrea L - (algranth); Subject: RE: [Histonet] histology in higher education Andi, Would you be willing to share the information on how to volunteer with this program? Thanks, Bonnie -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Grantham, Andrea L - (algranth) Sent: Wednesday, April 22, 2015 11:40 AM To: Subject: RE: [Histonet] histology in higher education For a few years I've been involved in a program called letters to a pre-scientist. The idea is to reach middle schoolers as they are being introduced to the sciences. They have pretty high goals at this time, they want to be doctors and astronauts and engineers but they are just starting to learn about these things. You become a pen pal/mentor of sorts and write letters to a child and they will write back to you. Last year I was writing to a boy in the Chicago area and this year it was a girl in LA. I always write about what I do and how important it is and include pictures of things like brain cells, muscle, fungus, bacteria and pictures of my lab. I always pick up a copy of the NSH coloring book and send it to them and tell them what they need to study to be a histotech and other than a hospital, where they can find a job. Of course we also tell them about other things like our families, pets, vacations, etc. at the same time. It's just a small thing but it plants a seed. Andi G. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: NY State Histology License
There is a grandfather clause if you were a histotech by april 24.1995. I asked ClIA recently when they came to inspect me, as I fell into this category but didn't know if you had to have the licence by then or just have been performing high complexity test at that time (my licence was later than that). The CLIA person couldn't decipher their own rules and was going to ask her supervisor. I never heard back. As for IHC any HT in our state can do it and CAP never brought it up when I worked at the hospital. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Gail Marcella Sent: Tuesday, April 21, 2015 8:48 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] NY State Histology License Hi - I've been a Histotech for 20+ years and got my Clinical Laboratory License in NY State when they required getting it. I don't have an Associates or Bachelor's degree but a Pathologist signed off for me. I have my HTASCP. I was told when I went for an interview in NY State that I couldn't gross small specimens or do IHC without an Associates or Bachelor degree in biology. I was not aware of these restrictions. I don't see anything on the NYS website. I was wondering if anyone else heard of this? Thanks - Gail ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Histonet Digest, Vol 137, Issue 30
Gail, The regulation is from CLIA '88. They list the requirements for that, and I have never heard of a grandfather clause. I could be wrong about though. NY state did not license HTL. I had a student who wanted to move there, and did not because he was going to have an HTL, not HT. He would only have been able to work clinical if he took both exams (HT and HTL). I even contacted the state society for clarification. Hopefully it has changed. I believe it is because they do not have any HTL programs in the state, so it was not included in the licensure bill. Sincerely, Toysha N. Mayer, D.H.Sc., MBA, HT (ASCP) Instructor/Education Coordinator Program in Histotechnology School of Health Professions UT M.D. Anderson Cancer Center 713.563-3481 Message: 8 Date: Thu, 23 Apr 2015 18:34:13 + From: Joelle Weaver joellewea...@hotmail.com Subject: RE: [Histonet] NY State Histology License To: Gail Marcella gmarce...@nj-urology.com, histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Message-ID: snt149-w8666703aac97b9793fdbc9d8...@phx.gbl Content-Type: text/plain; charset=iso-8859-1 Yes, CLIA stipulation. I think that there may be a grandfather clause, but not sure of the time frame. You could check the regulation on that. Joelle Weaver MAOM, HTL (ASCP) QIHC From: gmarce...@nj-urology.com To: histonet@lists.utsouthwestern.edu Date: Tue, 21 Apr 2015 11:47:58 -0400 Subject: [Histonet] NY State Histology License Hi - I've been a Histotech for 20+ years and got my Clinical Laboratory License in NY State when they required getting it. I don't have an Associates or Bachelor's degree but a Pathologist signed off for me. I have my HTASCP. I was told when I went for an interview in NY State that I couldn't gross small specimens or do IHC without an Associates or Bachelor degree in biology. I was not aware of these restrictions. I don't see anything on the NYS website. I was wondering if anyone else heard of this? Thanks - Gail ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Decalcification using EDTA
It is optional to decal in 4oC, but it is much better for preserve antigenicity and get good result from enzymatic staining. Similar way as you do fixation, you can choose RT or 4oC. Dorothy Hu Hello Histonetters Is it imperative OR optional to maintain samples at below 4 degrees celcius when using EDTA to decalcify bone samples Thank You ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] RE: Question on IHC billing
Yes, that is what we do. It is per specimen. First IHC 88342, additional are 88341 Joelle Weaver MAOM, HTL (ASCP) QIHC From: joyce.we...@emoryhealthcare.org To: jvick...@springfieldclinic.com; histonet@lists.utsouthwestern.edu Date: Thu, 23 Apr 2015 20:31:09 + CC: Subject: [Histonet] RE: Question on IHC billing Correct Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.we...@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Vickroy, James Sent: Thursday, April 23, 2015 4:19 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Question on IHC billing Let me see if I have this straight:If a pathologist orders an Hpylori stain on 2 blocks from the same specimen C1 and C2 we can only bill one 88342. If this correct.Obviously if he ordered addition different IHC stains we could change additional 88341's. Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois 62703 Office: 217-528-7541, Ext. 15121 Email: jvick...@springfieldclinic.commailto:jvick...@springfieldclinic.com This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you.___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] IHC and oven temperature
I remember reading that the preffered temperature was about 60 degrees Celsius. I think that this was in the Dako education guides if I'm not mistaken. If that is the case, the citation for the source is probably in that resource available as pdf from their website. Joelle Weaver MAOM, HTL (ASCP) QIHC From: tony.henw...@health.nsw.gov.au To: wdesalvo@outlook.com; preis...@mail.etsu.edu Date: Fri, 24 Apr 2015 09:43:59 + Subject: RE: [Histonet] IHC and oven temperature CC: histonet@lists.utsouthwestern.edu Hi temp drying shown to be a bad idea: Henwood, A., (2005) “Effect of Slide Drying at 80°C on Immunohistochemistry” J Histotechnol 28(1):45-46. Abstract Prolonged high temperature dry heating has been found to be deleterious to the immunohistochemical demonstration of several antigens in formalin-fixed, paraffin- embedded sections. Paraffin sections were dried at 80°C for 7 h and their immunoreactivity was compared with mirror sections dried for 1 h at 60°C. NCL-5D3, CMV, S100, HMB45, and CEA were quite labile to dry overheating whereas AElAE3, HBsAg, HBcAg, HSVII, EMA, chromogranin, and NSE were found to be quite resistant. It is recommended that coated slides (poly-L-lysine or aminopropyltriethoxysilane) and low-temperature drying (60°C) be routinely used for irnmunohistochemistry. From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of WILLIAM DESALVO [wdesalvo@outlook.com] Sent: Tuesday, 21 April 2015 1:56 AM To: Preiszner, Johanna Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] IHC and oven temperature Dry heat compared to wet heat. Do not dry your slides at high heat. You are removing water trapped between slide and paraffin section. Antigen retrieval is an entirely different process. So not try to combine the two processes Sent from my iPhone On Apr 20, 2015, at 8:48 AM, Preiszner, Johanna preis...@mail.etsu.edu wrote: Hi Netters, is there something wrong with this logic: If the tissue needs 95C for HIER, it's ok to dry the slides in an 82C oven. Of course I'll test it before I try it on real specimens, but maybe someone else already knows the answer... Thanks! Hanna Preiszner ETSU/QCOM ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet * This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. * ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
