[Histonet] re automatic cover slippers

[Steven Weston via Histonet]

We use the Dako cover slipper and provided the moving arm is kept free from 
dried mounting medium we have found this to be a terrific machine. It also has 
a really small footprint and so fits nicely into our fume hood. Can do about 
400 slides an hour and produces very few bubbles.

Steve Weston
Breathe-Well CRE
Lab Manager
0408990859




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Re: [Histonet] Histonet Digest, Vol 144, Issue 21: Rapid tissue Programs

[Steve McClain via Histonet]

Since processing is not the issue, first standardize fixation, eg 4-6 hours 
before processing or in the first reagent on processor. Change that first 
formalin every 200 blocks or so.  When well-fixed, small tissues will tolerate 
rapid processing. Finally, if your pathologists can read the slides and your 
immunostains work, then only make small changes. And ignore the CAP.

Steve A. McClain, MD


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[Histonet] Rapid tissue Programs

[Vickroy, James via Histonet]

Our latest CAP survey was returned today and although there are no major issues 
one possible improvement area is evident.   On all of the biopsies the area of 
"fixation/processing" was not rated as excellent.  The suggested possible 
reasons were:   fixation incomplete, nuclear bubbling artifact, tissue poorly 
processed.   The survey also said that many of the samples sent in throughout 
the country had similar  issues in the fixation/processing area most likely 
because of the rapid turnaround times and shortened processing times.   I am 
trying to be proactive here and see if we can adjust some times to improve the 
processing quality even though we have not had any complaints from the 
pathologists.   Of course we all know that other artifacts caused prior to the 
specimen arriving in the lab can also have an effect on the quality of the H 
slides.   Our fixation times should not be a factor so I have to conclude that 
maybe the rest of our processing times need to be adjusted.   Another
  factor that we have is that we use blue sponges for almost all of our 
tissues.  Our largest number of specimens are GI biopsies.If possible can 
anyone share with me their rapid processing schedules or simply the approximate 
times they have for each dehydration or clearing step. We do run a larger 
overnight tissue run on any biopsy or tissue that we feel is too large for the 
"rapid run".I am hesitant to run the biopsies routinely on the longer 
programs becase of over dehydration, etc. even though we do use an alcohol 
blend.

Any suggestions or similar experiences please share.Again our pathologists 
say everything looks great so I don't want to change much.

Jim

Jim Vickroy
Histology Manager
Springfield Clinic, Main Campus, East Building
1025 South 6th Street
Springfield, Illinois  62703
Office:  217-528-7541, Ext. 15121
Email:  jvick...@springfieldclinic.com



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Re: [Histonet] Rapid tissue Programs

[WILLIAM DESALVO via Histonet]

First suggestion is to remove the sponge. There will be carry over on 
biopsy/rapid tissue processor schedules. The sponges do require longer times to 
drain and exchange liquids. Your fixation times must be >6 hours. Make sure you 
are not applying too much heat to processing. Small biopsies are delicate and 
exchange of fluids/paraffin should not need physical elements to assist. 
Additionally, do not over dehydrate through alcohols.  You processor schedule 
will need to be validated, if you make time/ pressure/heat or reagent change. 
Good luck in the problem solving process.

Sent from my Windows Phone

From: Vickroy, James via Histonet
Sent: ‎11/‎24/‎2015 10:27 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Rapid tissue Programs

Our latest CAP survey was returned today and although there are no major issues 
one possible improvement area is evident.   On all of the biopsies the area of 
"fixation/processing" was not rated as excellent.  The suggested possible 
reasons were:   fixation incomplete, nuclear bubbling artifact, tissue poorly 
processed.   The survey also said that many of the samples sent in throughout 
the country had similar  issues in the fixation/processing area most likely 
because of the rapid turnaround times and shortened processing times.   I am 
trying to be proactive here and see if we can adjust some times to improve the 
processing quality even though we have not had any complaints from the 
pathologists.   Of course we all know that other artifacts caused prior to the 
specimen arriving in the lab can also have an effect on the quality of the H 
slides.   Our fixation times should not be a factor so I have to conclude that 
maybe the rest of our processing times need to be adjusted.   Another factor 
that we have is that we use blue sponges for almost all of our tissues.  Our 
largest number of specimens are GI biopsies.If possible can anyone share 
with me their rapid processing schedules or simply the approximate times they 
have for each dehydration or clearing step. We do run a larger overnight tissue 
run on any biopsy or tissue that we feel is too large for the "rapid run".I 
am hesitant to run the biopsies routinely on the longer programs becase of over 
dehydration, etc. even though we do use an alcohol blend.

Any suggestions or similar experiences please share.Again our pathologists 
say everything looks great so I don't want to change much.

Jim

Jim Vickroy
Histology Manager
Springfield Clinic, Main Campus, East Building
1025 South 6th Street
Springfield, Illinois  62703
Office:  217-528-7541, Ext. 15121
Email:  jvick...@springfieldclinic.com



This electronic message contains information from Springfield Clinic, LLP that 
may be confidential, privileged, and/or sensitive. This information is intended 
for the use of the individual(s) or entity(ies) named above. If you are not the 
intended recipient, be aware that disclosure, copying, distribution, or action 
taken on the contents of this information is strictly prohibited. If you have 
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Re: [Histonet] Rapid tissue Programs

[Rene J Buesa via Histonet]

You point out to several issues that I would like to address:1- "nuclear 
bubbling" has nothing to do with processing. This is a post-sectioning artifact 
appearing when there is water underneath the section when it is set to dry 
before staining. Just make sure you shake the slide with the section and set it 
to drain vertically before placing them into the oven to dry.2- sponges can be 
a source of problems influencing dehydration and even causing "marks" on the 
tissue that can later produce an artifact. Try to avoid sponges and use tissue 
paper to wrap the biopsies instead.3- fear not placing small biopsies in the 
overnight "long process". If the protocol is well balances you will have no 
problems. In all the years I oversaw tissue processing ine 2 labs I supervised 
(one with an excess of 35,000 cases/years and the other close to 200,000 
cases/year) I had only one protocol for everything, except for fatty tissues, 
breast and brain.The time in every reagent is not really the issue but the 
gradient you use. Abrupt changes (like starting with 90% alcohols) or not 
having "mixed steps" (1:1 ratios) between last alcohol and clearing agent or 
between clearing agent and melted paraffin are the real causes of the so called 
"harsh" processing.
If you go to http://www.histosearch.com/rene/html you will find my "standard" 
protocols.René 


On Tuesday, November 24, 2015 12:24 PM, "Vickroy, James via Histonet" 
 wrote:
 

 Our latest CAP survey was returned today and although there are no major 
issues one possible improvement area is evident.  On all of the biopsies the 
area of "fixation/processing" was not rated as excellent.  The suggested 
possible reasons were:  fixation incomplete, nuclear bubbling artifact, tissue 
poorly processed.  The survey also said that many of the samples sent in 
throughout the country had similar  issues in the fixation/processing area most 
likely because of the rapid turnaround times and shortened processing times.  I 
am trying to be proactive here and see if we can adjust some times to improve 
the processing quality even though we have not had any complaints from the 
pathologists.  Of course we all know that other artifacts caused prior to the 
specimen arriving in the lab can also have an effect on the quality of the H 
slides.  Our fixation times should not be a factor so I have to conclude that 
maybe the rest of our processing times need to be adjusted.  Another factor 
that we have is that we use blue sponges for almost all of our tissues.  Our 
largest number of specimens are GI biopsies.    If possible can anyone share 
with me their rapid processing schedules or simply the approximate times they 
have for each dehydration or clearing step. We do run a larger overnight tissue 
run on any biopsy or tissue that we feel is too large for the "rapid run".    I 
am hesitant to run the biopsies routinely on the longer programs becase of over 
dehydration, etc. even though we do use an alcohol blend.

Any suggestions or similar experiences please share.    Again our pathologists 
say everything looks great so I don't want to change much.

Jim

Jim Vickroy
Histology Manager
Springfield Clinic, Main Campus, East Building
1025 South 6th Street
Springfield, Illinois  62703
Office:  217-528-7541, Ext. 15121
Email:  jvick...@springfieldclinic.com



This electronic message contains information from Springfield Clinic, LLP that 
may be confidential, privileged, and/or sensitive. This information is intended 
for the use of the individual(s) or entity(ies) named above. If you are not the 
intended recipient, be aware that disclosure, copying, distribution, or action 
taken on the contents of this information is strictly prohibited. If you have 
received this electronic message in error, please notify the sender 
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[Histonet] Histology position

[Roy, Ryan via Histonet]

Hello Histonetters,

The Manchester VA Medical Center in Manchester NH currently has a critical need 
for a qualified Histologist.  It's an excellent position with great benefits.

If you are interested please review the link below.  Any further questions can 
be directed to contact Kevin Hall at (603)624-4366 ext. 6638.

Feel free to share the link.

https://www.usajobs.gov/GetJob/ViewDetails/422133100

This is not a third party posting.


Ryan Roy HTL (ASCP)
Histology Lab
Manchester VA
718 Symth Rd
Manchester NH 03104
(603) 624-4366 ex 6640


Disclosure: The content of this email does not reflect the policies, views or 
opinions of the VA.

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[Histonet] Masson's Trichrome Stain

[Camila Gomes via Histonet]

Dear all,

I did a Masson's Trichrome Stain in frozen liver samples with cancer and it
turns quite well to differentiate the collagen in the tumour from the
normal liver but I would also like to see better the nuclei detail and have
a less intense pink.
Here is the protocol that I am using with the Sigma kit:

(Fixation with 4%PFA for 10 minutes)

1- Bouin's Solution at room temperature over-night;
2- Wash in tap water until remove yellow color;
3- Weigert's Hematoxilin for 5 min;
4- Was in tap water for 5 min;
5- Rinse in deionized water;
6- Biebrich Scarlet-Acid Fucshin  for 5 minutes;
7- Rinse in deionized water;
8- Phosphomolybdic Acid for 5 minutes;
9- Aniline blue solution for 5 minutes;
10-Acetic acid 1% 1'30'';
11-Rinse in water, dehydrate, clear and mount.

All the solution are made at the moment of the staining. I have experience
with the staining but only in paraffin embedded sections and I would like
to know if there is any step to be careful or change with frozen sections.



Anyone can help me please?
Thank you,
Camila
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[Histonet] Not work related

[Lester Raff MD via Histonet]

Just for fun today

www.chicagonow.com/downsize-maybe/2015/11/we-want-to-be-on-hgtv-too/



Lester J. Raff, MD MBA
UroPartners
Medical Director Of Laboratory
2225 Enterprise Dr. Suite 2511
Westchester, Il 60154
Tel: 708-486-0076
Fax: 708-492-0203

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