Re: [Histonet] Melanin Bleach

[Tony Henwood (SCHN) via Histonet]

The KMnO4/oxalic acid sequence will weaken the tissue adherence.
If you do not have to use Peroxidase/DAB, then I would recommend alkaline 
phosphatase labelling (+red chromagen).

Remember KMnO4/oxalic acid can be deleterious to some antigen-antibody 
reactions. 
We found that LCA, CAM5.2, L26, alpha-fetoprotein, and NSE were labile, whilst 
alpha-1-antitrypsin, HBsAg, CMV, CEA, Thyroglobin and chromogranin showed 
decreased staining. EMA, desmin, HSVII, S100, GFAP, PSA, PAcP, Calcitonin, ACTH 
and Prolactin were found to be resistant to permanganate treatment.

Regards 
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) 
Principal Scientist, the Children's Hospital at Westmead
Adjunct Fellow, School of Medicine, University of Western Sydney 
Tel: 612 9845 3306 
Fax: 612 9845 3318 
Pathology Department
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 


-Original Message-
From: Heckford, Karen - SMMC-SF via Histonet 
[mailto:histonet@lists.utsouthwestern.edu] 
Sent: Wednesday, 11 May 2016 1:45 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Melanin Bleach

Good Morning,
I need some help.  Yesterday I bleached some heavily pigmented tissue.  I have 
to run some IHC's on them.   I bought the bleaching kit from American Master 
Tech.  I had to put the Permanganate for about 6 hours to get the melanin and 
then a couple of minutes in Oxalic Acid.  I had to let them set overnight 
because I could not get another IHC run in that day.   It looks like the tissue 
fell off during decloaking.I used Apex slides.   I rarely ever have to 
bleach anything here.  So I am not sure if I did this correctly.   I am 
thinking I need to bleach and do the run in the same day and not let them set 
over night in DiH20.

Thanks,


Karen Heckford HT ASCP CE
Lead Histology Technician
St. Mary's Medical Center
450 Stanyan St.
San Francisco, Ca. 94117
415-668-1000 ext. 6167
karen.heckf...@dignityhealth.org

  Caution:  This email message, including all content and attachments, is 
CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED.  The 
information contained in this email message is intended only for the use of the 
recipient(s) named above. If the reader of this message is not the intended 
recipient or an agent responsible for delivering it to the intended recipient, 
you have received this document in error.  Any further review, dissemination, 
distribution, or copying of this message is strictly prohibited.  If you have 
received this communication in error, please notify us  immediately by reply 
email.  Thank you."



___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

This message is intended for the addressee named and may contain confidential 
information. If you are not the intended recipient, please delete it and notify 
the sender.

Views expressed in this message are those of the individual sender, and are not 
necessarily the views of NSW Health or any of its entities.


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Cytology/Histology Staining Question

[Tony Henwood (SCHN) via Histonet]

Yep,
We do.
We have a separate dehydration sequence for PAP and other special stains 
(separate from the eosin dehydration sequence).

We have a Leica (used to be Vision Biosystems) Autostainer

Regards 
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) 
Principal Scientist, the Children's Hospital at Westmead
Adjunct Fellow, School of Medicine, University of Western Sydney 
Tel: 612 9845 3306 
Fax: 612 9845 3318 
Pathology Department
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 

-Original Message-
From: Mullen, Mary via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Wednesday, 11 May 2016 12:55 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Cytology/Histology Staining Question

Hello all,



I work in a small, low volume community hospital and was recently asked by a 
coworker why we do not just run both our cytology and histology slides on the 
same automated stainer (with their respective protocols).



What I am wanting to know is if there is anyone currently running both staining 
protocols on a single automated stainer using common alcohols/xylenes/water? 
What are the pros/cons? Has there been any cross-contamination issues?



We only run non-gyn cytology, all gyn cytology is sent out.







Thanks,



Mary K. Mullen, HTL(ASCP)CM
Histotechnologist
UPMC Northwest
Seneca, PA

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

This message is intended for the addressee named and may contain confidential 
information. If you are not the intended recipient, please delete it and notify 
the sender.

Views expressed in this message are those of the individual sender, and are not 
necessarily the views of NSW Health or any of its entities.


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Cryojane Oct artifact

[Jamie McGinness via Histonet]

I am getting a bubbly artifact on my tissue due to the OCT not being washed off 
the slide after putting it on a cryojane slide and flashing it and staining 
with H&E. Is there a trick to dissolve the OCT away

Sent from my iPhone

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Melanin Bleach

[Jay Lundgren via Histonet]

Cool trick Jeffrey!

On Tue, May 10, 2016 at 12:14 PM, Maxim Peshkov via Histonet <
histonet@lists.utsouthwestern.edu> wrote:

> Karen,
> The bleaching reagents will not compatible before IHC anyway.
> Some tips:
> 1. Do IHC-test as usual and counterstain nuclei with methylene blue
> Loeffler at 3-5 sec, only for
> profile of chromatine. The melanin will stain at green color.
> 2. Use any red color chromogen for IHC. It will be some contrast with
> brown melanine
> 3. Cut sections at 3 microns. Do all steps of IHC as usual, include DAB.
> All next steps do very gently:
> Before nuclear counterstain do bleaching procedure with 0.01% KMnO4 + 0.5%
> H2SO4 at 10 mins;
> gently rinse in DW 1 min;
> 0.5% oxalic acid 5-10 sec or up to bleaching;
> gently rinse in DW 1 min;
> counterstain nuclei and all other steps with regular manner of your lab.
> This solutions will not bleach DAB.
> Sections will not detach from slides.
>
> Hope this help.
> --
>  Maxim Peshkov
>  Russia
>  Taganrog. mailto:maxim...@mail.ru
>
>
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] P24

[Ginny Achstetter via Histonet]

Does anyone know of a supplier for P24 control slides?
Sent from my iPhone

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] p16 Nordi QC

[Eridana via Histonet]

There is a fantastic resource for antibody assessment and optimization and they 
have done p16 as well as many many other antibodies. Their results of testing 
is free on line.   p16 is http://www.nordiqc.org/Epitopes/p16/p16.htm from 2009 
with many different companies antibodies.

Here is the list of their epitopes http://www.nordiqc.org/epitopes.htm

Donna Harclerode, HT, ASCP, HTL, QIHC
Lead Histotechnologist
VA San Diego, CA

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Melanin Bleach

[Maxim Peshkov via Histonet]

Karen,
The bleaching reagents will not compatible before IHC anyway.
Some tips:
1. Do IHC-test as usual and counterstain nuclei with methylene blue Loeffler at 
3-5 sec, only for
profile of chromatine. The melanin will stain at green color.
2. Use any red color chromogen for IHC. It will be some contrast with brown 
melanine
3. Cut sections at 3 microns. Do all steps of IHC as usual, include DAB.
All next steps do very gently:
Before nuclear counterstain do bleaching procedure with 0.01% KMnO4 + 0.5% 
H2SO4 at 10 mins;
gently rinse in DW 1 min;
0.5% oxalic acid 5-10 sec or up to bleaching;
gently rinse in DW 1 min;
counterstain nuclei and all other steps with regular manner of your lab.
This solutions will not bleach DAB.
Sections will not detach from slides.

Hope this help.
-- 
 Maxim Peshkov
 Russia
 Taganrog. mailto:maxim...@mail.ru


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Open Position

[Gullifer, Dawn via Histonet]

Hi-

Job Opening:
OSU Histology Lab, LLC
Columbus, Ohio
Assistant Laboratory Manager for a small reference based laboratory.  Simple 
grossing and histology are performed.
Musts:
Graduate of a school of histology with a HT/HTL certification or equivalent.
A minimum of an associate's degree in a science related field with a minimum of 
24 semester hours (36 quarter hours) of biology, chemistry, physics and math.
3 years of full time histology technician experience in a clinical setting.
3 years of lead, supervisor or manager experience in an anatomic 
pathology/histology lab strongly desired.

Contact:  dawn.gulli...@osumc.edu


Dawn Gullifer, BS, HT (ASCP)
Clinical Laboratory and Outreach Manager
Department of Pathology
The Ohio State University Wexner Medical Center
OSU Physicians Inc
OSU Histology Lab, LLC
1507 Chambers Rd.Columbus.Ohio.43212
614.293.0358 Office
614.293.0345 Lab
dawn.gulli...@osumc.edu

CONFIDENTIALITY NOTICE: This e-mail, including attachments, may contain 
information that is physician-patient privileged, proprietary or otherwise 
confidential. If you are not the intended recipient or his or her authorized 
agent, use and disclosure of this message are prohibited. Any dissemination, 
distribution or copying of this e-mail is prohibited. If you received this 
e-mail in error, please notify the sender by replying to this e-mail and 
immediately delete the message and any attachments.

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Cytology/Histology Staining Question

[Hannen, Valerie via Histonet]

Mary,

We run both protocols on the same stainer, however, each protocol has it own 
set of reagents except for the common water wells and the two xylenes at the 
end before the final xylene.  We have the Leica ST 5020, which has 36 wells not 
including the 2 oven wells.

Hope this helps.


Valerie Hannen,MLT(ASCP),HTL,SU (FL)
Section Chief, Histology
Parrish Medical Center
951 N. Washington Ave.
Titusville,Florida 32796
T: (321)268-6333 ext. 7506
F: (321) 268-6149
valerie.han...@parrishmed.com
www.parrishmed.com


-Original Message-
From: Mullen, Mary via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Tuesday, May 10, 2016 10:55 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Cytology/Histology Staining Question

Hello all,



I work in a small, low volume community hospital and was recently asked by a 
coworker why we do not just run both our cytology and histology slides on the 
same automated stainer (with their respective protocols).



What I am wanting to know is if there is anyone currently running both staining 
protocols on a single automated stainer using common alcohols/xylenes/water? 
What are the pros/cons? Has there been any cross-contamination issues?



We only run non-gyn cytology, all gyn cytology is sent out.







Thanks,



Mary K. Mullen, HTL(ASCP)CM
Histotechnologist
UPMC Northwest
Seneca, PA

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
==
"This email is intended solely for the use of the individual to
whom it is addressed and may contain information that is
privileged, confidential or otherwise exempt from disclosure
under applicable law. If the reader of this email is not the
intended recipient or the employee or agent responsible for
delivering the message to the intended recipient, you are
hereby notified that any dissemination, distribution, or
copying of this communication is strictly prohibited. If you
have received this communication in error, please immediately
delete this message. Thank you"
==


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Cytology/Histology Staining Question (Mullen, Mary)

[O'Donnell, Lynn M. via Histonet]

This is the CAP regulation that talks about cross contamination.


CYP.04150 Cross-Contamination Phase I
There is a written procedure to prevent cross-contamination of specimens during
processing and staining.
NOTE: Procedures must prevent cross-contamination between gynecologic and 
non-gynecologic
specimens.
Also, procedures must prevent contamination among non-gynecologic cases when 
highly
cellular specimens are processed. Methods to minimize this potential problem 
may include
cytocentrifuge, filter, and monolayer preparations. Direct smears made from the 
sediment of
highly cellular cases should be stained after the other cases, and the staining 
fluids must be
changed or filtered between each of the highly cellular cases. One procedure to 
detect highly
cellular specimens is to use a toluidine blue, or other rapid stain, on a wet 
preparation. One
procedure to detect possible contamination is to insert a clean blank slide in 
each staining run
and examine it for contamination.
REFERENCES
1) Department of Health and Human Services, Centers for Medicare and Medicaid 
Services. Clinical laboratory improvement
amendments of 1988; final rule. Fed Register. 2003(Jan 24):7169 
[42CFR493.1274(b)(2-3)]




Lynn M. O'Donnell, CT (ASCP), MHA l Technical Specialist, Cytology
Danbury Hospital l lynn.o'donn...@wchn.org
tel: 203-739-6704  Fax: 203-739-6034




-Original Message-
From: T H via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Tuesday, May 10, 2016 13:46
To: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Cytology/Histology Staining Question (Mullen, Mary)

Hey Mary,

The problem is not the machine, it is the reagents sharing that is the issue.  
You can use different reagents and protocols for the Histo and Cyto slides and 
on the same instrument.  You might even get away with changing your Alcohols 
and filtering everything else and see how that works.  Try it and run some 
blank slides through the stainer and see if anything is there from the cytology 
specimens.

I would personally have two separate sets of staining reagents to be on the 
safe side.

Good luck!!

Tim


Message: 7
Date: Tue, 10 May 2016 14:54:55 +
From: "Mullen, Mary" 
To: "histonet@lists.utsouthwestern.edu"

Subject: [Histonet] Cytology/Histology Staining Question
Message-ID:
<374dc72e6b29d44086f8ff3289351b2508823...@msxmbxnsprd39.acct.upmchs.net>

Content-Type: text/plain; charset="iso-8859-1"

Hello all,



I work in a small, low volume community hospital and was recently asked by a 
coworker why we do not just run both our cytology and histology slides on the 
same automated stainer (with their respective protocols).



What I am wanting to know is if there is anyone currently running both staining 
protocols on a single automated stainer using common alcohols/xylenes/water? 
What are the pros/cons? Has there been any cross-contamination issues?



We only run non-gyn cytology, all gyn cytology is sent out.







Thanks,



Mary K. Mullen, HTL(ASCP)CM
Histotechnologist
UPMC Northwest
Seneca, PA



___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Cytology/Histology Staining Question (Mullen, Mary)

[T H via Histonet]

Hey Mary,

The problem is not the machine, it is the reagents sharing that is the issue.  
You can use different reagents and protocols for the Histo and Cyto slides and 
on the same instrument.  You might even get away with changing your Alcohols 
and filtering everything else and see how that works.  Try it and run some 
blank slides through the stainer and see if anything is there from the cytology 
specimens.

I would personally have two separate sets of staining reagents to be on the 
safe side.

Good luck!!

Tim


Message: 7
Date: Tue, 10 May 2016 14:54:55 +
From: "Mullen, Mary" 
To: "histonet@lists.utsouthwestern.edu"

Subject: [Histonet] Cytology/Histology Staining Question
Message-ID:
<374dc72e6b29d44086f8ff3289351b2508823...@msxmbxnsprd39.acct.upmchs.net>

Content-Type: text/plain; charset="iso-8859-1"

Hello all,



I work in a small, low volume community hospital and was recently asked by a 
coworker why we do not just run both our cytology and histology slides on the 
same automated stainer (with their respective protocols).



What I am wanting to know is if there is anyone currently running both staining 
protocols on a single automated stainer using common alcohols/xylenes/water? 
What are the pros/cons? Has there been any cross-contamination issues?



We only run non-gyn cytology, all gyn cytology is sent out.







Thanks,



Mary K. Mullen, HTL(ASCP)CM
Histotechnologist
UPMC Northwest
Seneca, PA



___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Melanin Bleach

[Rene J Buesa via Histonet]

You are right. Bleaching is a "rough" procedure for the "survival" of sections 
and if on top of that you left the section overnight in DiH2O that is a recipe 
for disaster, as the one you experienced. Try to do the whole procedure during 
the same day.Additionally it seems to me that 6h in potassium permanganate is 
too much, you should check the condition of the sections every hour trying to 
minimize KMnO4 action the least time possible.René 

On Tuesday, May 10, 2016 12:10 PM, "Heckford, Karen - SMMC-SF via Histonet" 
 wrote:
 

 Good Morning,
I need some help.  Yesterday I bleached some heavily pigmented tissue.  I have 
to run some IHC's on them.  I bought the bleaching kit from American Master 
Tech.  I had to put the Permanganate for about 6 hours to get the melanin and 
then a couple of minutes in Oxalic Acid.  I had to let them set overnight 
because I could not get another IHC run in that day.  It looks like the tissue 
fell off during decloaking.    I used Apex slides.  I rarely ever have to 
bleach anything here.  So I am not sure if I did this correctly.  I am thinking 
I need to bleach and do the run in the same day and not let them set over night 
in DiH20.

Thanks,


Karen Heckford HT ASCP CE
Lead Histology Technician
St. Mary's Medical Center
450 Stanyan St.
San Francisco, Ca. 94117
415-668-1000 ext. 6167
karen.heckf...@dignityhealth.org
                                                                                
  Caution:  This email message, including all content and attachments, is 
CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED.  The 
information contained in this email message is intended only for the use of the 
recipient(s) named above. If the reader of this message is not the intended 
recipient or an agent responsible for delivering it to the intended recipient, 
you have received this document in error.  Any further review, dissemination, 
distribution, or copying of this message is strictly prohibited.  If you have 
received this communication in error, please notify us  immediately by reply 
email.  Thank you."



___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


  
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Melanin Bleach

[Jeffrey Robinson via Histonet]

Hi Karen-  I have also had problems getting tissue being stained for IHC 
markers to adhere to the slide.  The Potassium permanganate is really harse on 
the tissue (if you can get the sections to stay on).  Here is a trick I picked 
up at an NSH lecture that I have used successfully several times.

Run your IHC stains as usual.  Rinse well in water.  Do not counterstain with 
hematoxylin.  Stain with DiffQuik II for 30 seconds.  Rinse in water for a 
short period of time.  Differentiate with 10 dips each in 2 changes of 95% ETOH 
and 2 changes of 100% ETOH and then clear in xylene and coverslip.
Results:  melanin pigment will turn green.  Brown DAB stain will be unaffected. 
 Other tissue elements will be stained a medium blue color.
This method will also work for other Special Stains but I have not attempted to 
modify this method for use on "Red" stains.

Jeff Robinson, Senior Histotechnologist, Sierra Pathology Lab, Clovis, CA.

-Original Message-
From: Heckford, Karen - SMMC-SF via Histonet 
[mailto:histonet@lists.utsouthwestern.edu]
Sent: Tuesday, May 10, 2016 8:45 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Melanin Bleach

Good Morning,
I need some help.  Yesterday I bleached some heavily pigmented tissue.  I have 
to run some IHC's on them.   I bought the bleaching kit from American Master 
Tech.  I had to put the Permanganate for about 6 hours to get the melanin and 
then a couple of minutes in Oxalic Acid.  I had to let them set overnight 
because I could not get another IHC run in that day.   It looks like the tissue 
fell off during decloaking.I used Apex slides.   I rarely ever have to 
bleach anything here.  So I am not sure if I did this correctly.   I am 
thinking I need to bleach and do the run in the same day and not let them set 
over night in DiH20.

Thanks,


Karen Heckford HT ASCP CE
Lead Histology Technician
St. Mary's Medical Center
450 Stanyan St.
San Francisco, Ca. 94117
415-668-1000 ext. 6167
karen.heckf...@dignityhealth.org

  Caution:  This email message, including all content and attachments, is 
CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED.  The 
information contained in this email message is intended only for the use of the 
recipient(s) named above. If the reader of this message is not the intended 
recipient or an agent responsible for delivering it to the intended recipient, 
you have received this document in error.  Any further review, dissemination, 
distribution, or copying of this message is strictly prohibited.  If you have 
received this communication in error, please notify us  immediately by reply 
email.  Thank you."



___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


This email and attachments may contain PHI that is privileged and confidential 
and is not intended for any unauthorized person. If you, the reader, are not 
the intended recipient you are hereby notified that any dissemination, 
distribution or copying of this communication is strictly prohibited. Do not 
read the email but instead reply to the sender and destroy the message and any 
attachments. Thank you.

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Melanin Bleach

[Heckford, Karen - SMMC-SF via Histonet]

Good Morning,
I need some help.  Yesterday I bleached some heavily pigmented tissue.  I have 
to run some IHC's on them.   I bought the bleaching kit from American Master 
Tech.  I had to put the Permanganate for about 6 hours to get the melanin and 
then a couple of minutes in Oxalic Acid.  I had to let them set overnight 
because I could not get another IHC run in that day.   It looks like the tissue 
fell off during decloaking.I used Apex slides.   I rarely ever have to 
bleach anything here.  So I am not sure if I did this correctly.   I am 
thinking I need to bleach and do the run in the same day and not let them set 
over night in DiH20.

Thanks,


Karen Heckford HT ASCP CE
Lead Histology Technician
St. Mary's Medical Center
450 Stanyan St.
San Francisco, Ca. 94117
415-668-1000 ext. 6167
karen.heckf...@dignityhealth.org

  Caution:  This email message, including all content and attachments, is 
CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED.  The 
information contained in this email message is intended only for the use of the 
recipient(s) named above. If the reader of this message is not the intended 
recipient or an agent responsible for delivering it to the intended recipient, 
you have received this document in error.  Any further review, dissemination, 
distribution, or copying of this message is strictly prohibited.  If you have 
received this communication in error, please notify us  immediately by reply 
email.  Thank you."



___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] ASCP Certified Pathologist Assistant Job Opening-

[Melissa Owens via Histonet]

Hello,

I have a Full Time/Permanent job opening for an ASCP certified Pathologist 
Assistant in Ohio. Please contact me for details. Have a great day!

Melissa Owens
President, Laboratory Staffing
Allied Search Partners


T: 888.388.7571 ext. 102

F: 888.388.7572

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Cytology/Histology Staining Question

[Rene J Buesa via Histonet]

I would be concerned with potential cross-contamination. In my lab we had 2 
staining instruments, one for cytology and other for histology.René 

On Tuesday, May 10, 2016 10:59 AM, "Mullen, Mary via Histonet" 
 wrote:
 

 Hello all,



I work in a small, low volume community hospital and was recently asked by a 
coworker why we do not just run both our cytology and histology slides on the 
same automated stainer (with their respective protocols).



What I am wanting to know is if there is anyone currently running both staining 
protocols on a single automated stainer using common alcohols/xylenes/water? 
What are the pros/cons? Has there been any cross-contamination issues?



We only run non-gyn cytology, all gyn cytology is sent out.







Thanks,



Mary K. Mullen, HTL(ASCP)CM
Histotechnologist
UPMC Northwest
Seneca, PA

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


  
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Cytology/Histology Staining Question

[Mullen, Mary via Histonet]

Hello all,



I work in a small, low volume community hospital and was recently asked by a 
coworker why we do not just run both our cytology and histology slides on the 
same automated stainer (with their respective protocols).



What I am wanting to know is if there is anyone currently running both staining 
protocols on a single automated stainer using common alcohols/xylenes/water? 
What are the pros/cons? Has there been any cross-contamination issues?



We only run non-gyn cytology, all gyn cytology is sent out.







Thanks,



Mary K. Mullen, HTL(ASCP)CM
Histotechnologist
UPMC Northwest
Seneca, PA

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] P16

[Charles Riley via Histonet]

Has anyone found a way to do p16 staining without purchasing anything from
Ventana?

My company wants to do P16 but refuses to by any ventana products and I
have explained it is a waste of money to keep testing the antibodies from
all the other companies.

-- 

Charles Riley HT(ASCP)CM

Histopathology Coordinator/ Mohs
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet