Re: [Histonet] No Ribbon
I might get some individuals upset but we really need to watch what we do in the histology lab and how that effects the precision of our process. When you breath on the block you are basically generating a thicker section, it's not a good practice to keep. Trust me I used to do this in the past. Thicker sections especially for IHC will generate a more intense stain, most individuals may think that that should not be an issue but it is - in the clinical setting especially when you are staining for therapeutic markers such as Her2 and PD-L1 - 510K cleared kits for these recommend specific section thickness settings and in the research setting if you are running any type of analysis on a batch of samples. If your microtome is set at 4 microns and you are breathing on the block you are not cutting at 4 microns, you are cutting thicker than that and a thicker section will increase staining intensity and therefore alter the results. Just my two cents. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell l...@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -Original Message- From: Caroline Miller via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Tuesday, April 11, 2017 11:59 AM To: Paula Keene Pierce Cc: Histonet Histonet Subject: Re: [Histonet] No Ribbon Yes, +1 to Paula, also: - I often found they are too sharp (!!) to ribbon so a gentle wipe (in the direction of the bevel) with a kimwipe helps - Also 'huffing' slightly on the block will warm the paraffin and both smooth out the wrinkles as well as make the sections stick together :) mills On Tue, Apr 11, 2017 at 10:37 AM, Paula Keene Pierce via Histonet < histonet@lists.utsouthwestern.edu> wrote: > Hi, > the blades are packed with a tiny, bit of oil. This can cause you to > not obtain a ribbon. > Try gently cleaning with xylene, followed by alcohol before > sectioning. Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur > Pathology, Inc.5830 N Blue Lake DriveNorman, OK 73069PH > 405-759-3953FAX 405-759-7513www.excaliburpathology.com > > From: Gareth Davis via Histonet > > To: "Histonet@lists.utsouthwestern.edu" utsouthwestern.edu> > Sent: Tuesday, April 11, 2017 11:55 AM > Subject: [Histonet] No Ribbon > > I feel like I should be able to figure this one out, but I have been > having trouble with getting a ribbon for a while now. I have a Leica > Microtome > RM2125 and I use either a AccuEdge blade (I get one block to cut then > it starts to compress) or a StatCut blade (sections do not stick > together at all), both High Profile. The angle is about 4 microns. I > change the angle a lot to see if that makes a difference and it > doesn't. There are so many factors that could be the issue, I think > lack of humidity may be the main one. So, suggestions please!! > > -- > Ms. Gareth B. Davis, B.S., HT, QIHC (ASCP)cm Yuma Gastroenterology > Yuma, AZ 85364 > 928-248-5259 > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Caroline Miller (mills) Director of Histology 3Scan.com 415 2187297 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __ This email has been scanned by the Symantec Email Security.cloud service. For more information please visit http://www.symanteccloud.com __ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] No Ribbon
Point taken, thanks Elizabeth. My clinical days are long gone, but that is an important consideration. yours, mills On Tue, Apr 11, 2017 at 11:13 AM, Elizabeth Chlipala wrote: > I might get some individuals upset but we really need to watch what we do > in the histology lab and how that effects the precision of our process. > When you breath on the block you are basically generating a thicker > section, it's not a good practice to keep. Trust me I used to do this in > the past. Thicker sections especially for IHC will generate a more intense > stain, most individuals may think that that should not be an issue but it > is - in the clinical setting especially when you are staining for > therapeutic markers such as Her2 and PD-L1 - 510K cleared kits for these > recommend specific section thickness settings and in the research setting > if you are running any type of analysis on a batch of samples. If your > microtome is set at 4 microns and you are breathing on the block you are > not cutting at 4 microns, you are cutting thicker than that and a thicker > section will increase staining intensity and therefore alter the results. > > Just my two cents. > > Liz > > Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC > Premier Laboratory, LLC > PO Box 18592 > Boulder, CO 80308 > (303) 682-3949 office > (303) 682-9060 fax > (303) 881-0763 cell > l...@premierlab.com > www.premierlab.com > > Ship to Address: > > Premier Laboratory, LLC > 1567 Skyway Drive, Unit E > Longmont, CO 80504 > > > -Original Message- > From: Caroline Miller via Histonet [mailto:histonet@lists. > utsouthwestern.edu] > Sent: Tuesday, April 11, 2017 11:59 AM > To: Paula Keene Pierce > Cc: Histonet Histonet > Subject: Re: [Histonet] No Ribbon > > Yes, +1 to Paula, also: > - I often found they are too sharp (!!) to ribbon so a gentle wipe (in the > direction of the bevel) with a kimwipe helps > - Also 'huffing' slightly on the block will warm the paraffin and both > smooth out the wrinkles as well as make the sections stick together > > :) > > mills > > On Tue, Apr 11, 2017 at 10:37 AM, Paula Keene Pierce via Histonet < > histonet@lists.utsouthwestern.edu> wrote: > > > Hi, > > the blades are packed with a tiny, bit of oil. This can cause you to > > not obtain a ribbon. > > Try gently cleaning with xylene, followed by alcohol before > > sectioning. Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur > > Pathology, Inc.5830 N Blue Lake DriveNorman, OK 73069PH > > 405-759-3953FAX 405-759-7513www.excaliburpathology.com > > > > From: Gareth Davis via Histonet > > > > To: "Histonet@lists.utsouthwestern.edu" > utsouthwestern.edu> > > Sent: Tuesday, April 11, 2017 11:55 AM > > Subject: [Histonet] No Ribbon > > > > I feel like I should be able to figure this one out, but I have been > > having trouble with getting a ribbon for a while now. I have a Leica > > Microtome > > RM2125 and I use either a AccuEdge blade (I get one block to cut then > > it starts to compress) or a StatCut blade (sections do not stick > > together at all), both High Profile. The angle is about 4 microns. I > > change the angle a lot to see if that makes a difference and it > > doesn't. There are so many factors that could be the issue, I think > > lack of humidity may be the main one. So, suggestions please!! > > > > -- > > Ms. Gareth B. Davis, B.S., HT, QIHC (ASCP)cm Yuma Gastroenterology > > Yuma, AZ 85364 > > 928-248-5259 > > ___ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > ___ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > -- > Caroline Miller (mills) > Director of Histology > 3Scan.com > 415 2187297 > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > __ > This email has been scanned by the Symantec Email Security.cloud service. > For more information please visit http://www.symanteccloud.com > __ > -- Caroline Miller (mills) Director of Histology 3Scan.com 415 2187297 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] No Ribbon
Yes, +1 to Paula, also: - I often found they are too sharp (!!) to ribbon so a gentle wipe (in the direction of the bevel) with a kimwipe helps - Also 'huffing' slightly on the block will warm the paraffin and both smooth out the wrinkles as well as make the sections stick together :) mills On Tue, Apr 11, 2017 at 10:37 AM, Paula Keene Pierce via Histonet < histonet@lists.utsouthwestern.edu> wrote: > Hi, > the blades are packed with a tiny, bit of oil. This can cause you to not > obtain a ribbon. > Try gently cleaning with xylene, followed by alcohol before > sectioning. Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur > Pathology, Inc.5830 N Blue Lake DriveNorman, OK 73069PH 405-759-3953FAX > 405-759-7513www.excaliburpathology.com > > From: Gareth Davis via Histonet > To: "Histonet@lists.utsouthwestern.edu" utsouthwestern.edu> > Sent: Tuesday, April 11, 2017 11:55 AM > Subject: [Histonet] No Ribbon > > I feel like I should be able to figure this one out, but I have been having > trouble with getting a ribbon for a while now. I have a Leica Microtome > RM2125 and I use either a AccuEdge blade (I get one block to cut then it > starts to compress) or a StatCut blade (sections do not stick together at > all), both High Profile. The angle is about 4 microns. I change the angle > a lot to see if that makes a difference and it doesn't. There are so many > factors that could be the issue, I think lack of humidity may be the main > one. So, suggestions please!! > > -- > Ms. Gareth B. Davis, B.S., HT, QIHC (ASCP)cm > Yuma Gastroenterology > Yuma, AZ 85364 > 928-248-5259 > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Caroline Miller (mills) Director of Histology 3Scan.com 415 2187297 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] No Ribbon
Hi, the blades are packed with a tiny, bit of oil. This can cause you to not obtain a ribbon. Try gently cleaning with xylene, followed by alcohol before sectioning. Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N Blue Lake DriveNorman, OK 73069PH 405-759-3953FAX 405-759-7513www.excaliburpathology.com From: Gareth Davis via Histonet To: "Histonet@lists.utsouthwestern.edu" Sent: Tuesday, April 11, 2017 11:55 AM Subject: [Histonet] No Ribbon I feel like I should be able to figure this one out, but I have been having trouble with getting a ribbon for a while now. I have a Leica Microtome RM2125 and I use either a AccuEdge blade (I get one block to cut then it starts to compress) or a StatCut blade (sections do not stick together at all), both High Profile. The angle is about 4 microns. I change the angle a lot to see if that makes a difference and it doesn't. There are so many factors that could be the issue, I think lack of humidity may be the main one. So, suggestions please!! -- Ms. Gareth B. Davis, B.S., HT, QIHC (ASCP)cm Yuma Gastroenterology Yuma, AZ 85364 928-248-5259 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] No Ribbon
Yes, I use ice for my blocks and get them as cold as possible. It is a high profile blade holder. The paraffin is good for embedding and processing. I was told to use a low angle for Statcut blades, but I will try it at 10. Thanks -- Ms. Gareth B. Davis, B.S., HT, QIHC (ASCP)cm Yuma Gastroenterology Yuma, AZ 85364 928-248-5259 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] NY Histotech
Hi Histonetters! Do you know of anyone who has a NYS laboratory license? I have a fantastic private lab client on Long Island (1hr outside of Manhattan). They are looking for someone to fill their Full-Time, permanent, Day-Shift Histotech role! It is a great environment in a great location, and won't be available very long! Please send over an updated resume and let me know the best time to get a hold of you. Sincerely, *Angie Laparidis*Healthcare Recruiter K.A. Recruiting, Inc. 10 Post Office Square, 8th Floor South, Boston, MA, 02109 W: 617.746-2744 (*please note this is a new number*) F: (617) 507-8009 an...@ka-recruiting.com Our openings are updated daily at www.ka-recruiting.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] No Ribbon
I feel like I should be able to figure this one out, but I have been having trouble with getting a ribbon for a while now. I have a Leica Microtome RM2125 and I use either a AccuEdge blade (I get one block to cut then it starts to compress) or a StatCut blade (sections do not stick together at all), both High Profile. The angle is about 4 microns. I change the angle a lot to see if that makes a difference and it doesn't. There are so many factors that could be the issue, I think lack of humidity may be the main one. So, suggestions please!! -- Ms. Gareth B. Davis, B.S., HT, QIHC (ASCP)cm Yuma Gastroenterology Yuma, AZ 85364 928-248-5259 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
