[Histonet] About Amoebae infected Tissue for Undergraduate student Practical class

[Nilufa Sultana via Histonet]

Dear Histo-netter,

It will be appreciated if I can get information for the following-

1.   Any source to get Amoebae infected Animal/humane tissue/Block to cut 
about 800 slides for the undergraduate Anatomy Pathology Practical class for 
staining with PAS

2.   Any method to make control block with the infection of Amoebae to the 
Animal tissue



Thank you so much .



With kind regards

Nilufa Sultana

Histology and Anatomy pathology

University Of Technology, Sydney

Australia

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Re: [Histonet] Deplastifying MMA

[Dr. Michael Gudo (Morphisto GmbH) via Histonet]

Hi Dorothy,
Hi Reuel,

MMA embedding and deplastination is of course a very difficult process and many 
protocols are discussed, but only a few work well. So in general: real 
deplastination cannot be performed by xylene or acetone alone, these solutions 
are just a pretreatment. The best way for remove MMA from section is a 
procedure starting with xylene, then going into acetone (2 times) and then into 
MEA (Methoxyethylacetate) for at least 2-3 times for a minimum of 10-20 
minutes. You can also directly go into MEA, however, pretreatment with xylene 
or acetone helps to reduce the steps and volume of MEA you need.

Which kind of MMA do you use?

In our lab we do resin embedding in Technovit 9100, which is very well 
established MMA that provides good results for thin sections between around a 
few microns, but and also for thicker sections and even for 
cutting-grinding-techniques. 

We can also provide you with several protocols for infiltration, deplastination 
and staining of Technovit-sections.
You find some further information and also some hands-on-descriptions for all 
histology-technovits in this brochure:

http://www.morphisto.de/fileadmin/pdf/Brochure_Technovit_Histology_EN.pdf 


If you have any further questions, just contact me personally.
With best regards
Michael

P.S. Further information about the technovits you can also find here:
http://www.morphisto.de/en/resin-histology/technovit-resins/ 



> Am 14.02.2018 um 20:45 schrieb Dorothy Hu via Histonet 
>  >:
> 
>> 
>> Hi  Reuel
>> 
> 
> We had the same thing as you. It was caused by bad MMA, I tested. Write
> down the lot # and ask refund. Test new lot # 1st of MMA in the future. It
> was very frustratingI know.
> Best luck.
> 
> Dorothy
> 
> 
> 
> 
> 
>> Message: 2
>> Date: Mon, 12 Feb 2018 21:07:15 +
>> From: Reuel Cornelia > >
>> To: "histonet@lists.utsouthwestern.edu 
>> "
>>> >
>> Subject: [Histonet] Deplastifying MMA
>> Message-ID:
>>> namprd17.prod.outlook.com >
>> 
>> Content-Type: text/plain; charset="iso-8859-1"
>> 
>> Hello histonetters,
>> 
>> I have a problem with my MMA plastic section on my slide. I could not
>> remove the plastic MMA even if I leave the section slides in Xylene for
>> several days with heat incubation at 60 degrees. This is the first time
>> that happened to my section for several years of doing the same thing over.
>> I was thinking that it was my Xylene lot number so I tried to change it but
>> still does not remove the plastic, and then Itried different solvent
>> Acetone, and Ethylene glycol Monoethyl ether but still it does not work.
>> Can I ask for help if anyone knows what was going on and what would be the
>> best way to remove this plastic from my section?
>> 
>> Just to give you my embedding solution was MMA -94% , dibutly phthalate-
>> 5% and perkadox 16- 0.5%. This have been my solution for years and I do not
>> have any problem with the removal of plastic section until now. I was
>> thinking that my MMA (M55909) different lot number from Sigma Aldrich may
>> have cause this because even I tried to use the MMA to dissolve my plastic
>> it does not work.
>> 
>> 
>> Thank you for and any opinions or protocols are greatly appreciated.
>> 
>> 
>> Reuel Cornelia
>> 
>> TSRH
>> 
>> 214-559-7766
>> 
>> 
> ___
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MORPHISTO GmbH
PD Dr. phil. nat. Michael Gudo
Weismüllerstr. 45
60314 Frankfurt am Main
Telefon:069 / 400 3019 - 62
Telefax:069 / 400 3019 - 64

E-Mail: michael.g...@morphisto.de 
Internet: http://www.morphisto.de/ 

Vertretungsberechtigter Geschäftsführer: Dr. Michael Gudo

Registergericht: Amtsgericht Frankfurt
Registernummer: HRB 74954
Umsatzsteuer-Identifikationsnummer gemäß § 27 a Umsatzsteuergesetz: DE243397199

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Re: [Histonet] [EXTERNAL] Deplastifying MMA

[Garcia, Lori, M.Sc. via Histonet]

Hi Reuel,

One thing you could try is THF (tetrahydrofuran). We have used it in the past 
to rapidly dissolve the MMA on slides.

Good luck,
Lori

-Original Message-
From: Dorothy Hu via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Wednesday, February 14, 2018 11:46 AM
To: histonet@lists.utsouthwestern.edu
Subject: [EXTERNAL] [Histonet] Deplastifying MMA

>
> Hi  Reuel
>

We had the same thing as you. It was caused by bad MMA, I tested. Write down 
the lot # and ask refund. Test new lot # 1st of MMA in the future. It was very 
frustratingI know.
Best luck.

Dorothy





> Message: 2
> Date: Mon, 12 Feb 2018 21:07:15 +
> From: Reuel Cornelia 
> To: "histonet@lists.utsouthwestern.edu"
> 
> Subject: [Histonet] Deplastifying MMA
> Message-ID:
>  namprd17.prod.outlook.com>
>
> Content-Type: text/plain; charset="iso-8859-1"
>
> Hello histonetters,
>
> I have a problem with my MMA plastic section on my slide. I could not
> remove the plastic MMA even if I leave the section slides in Xylene
> for several days with heat incubation at 60 degrees. This is the first
> time that happened to my section for several years of doing the same thing 
> over.
> I was thinking that it was my Xylene lot number so I tried to change
> it but still does not remove the plastic, and then Itried different
> solvent Acetone, and Ethylene glycol Monoethyl ether but still it does not 
> work.
> Can I ask for help if anyone knows what was going on and what would be
> the best way to remove this plastic from my section?
>
> Just to give you my embedding solution was MMA -94% , dibutly
> phthalate- 5% and perkadox 16- 0.5%. This have been my solution for
> years and I do not have any problem with the removal of plastic
> section until now. I was thinking that my MMA (M55909) different lot
> number from Sigma Aldrich may have cause this because even I tried to
> use the MMA to dissolve my plastic it does not work.
>
>
> Thank you for and any opinions or protocols are greatly appreciated.
>
>
> Reuel Cornelia
>
> TSRH
>
> 214-559-7766
>
>
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[Histonet] Deplastifying MMA

[Dorothy Hu via Histonet]

>
> Hi  Reuel
>

We had the same thing as you. It was caused by bad MMA, I tested. Write
down the lot # and ask refund. Test new lot # 1st of MMA in the future. It
was very frustratingI know.
Best luck.

Dorothy





> Message: 2
> Date: Mon, 12 Feb 2018 21:07:15 +
> From: Reuel Cornelia 
> To: "histonet@lists.utsouthwestern.edu"
> 
> Subject: [Histonet] Deplastifying MMA
> Message-ID:
>  namprd17.prod.outlook.com>
>
> Content-Type: text/plain; charset="iso-8859-1"
>
> Hello histonetters,
>
> I have a problem with my MMA plastic section on my slide. I could not
> remove the plastic MMA even if I leave the section slides in Xylene for
> several days with heat incubation at 60 degrees. This is the first time
> that happened to my section for several years of doing the same thing over.
> I was thinking that it was my Xylene lot number so I tried to change it but
> still does not remove the plastic, and then Itried different solvent
> Acetone, and Ethylene glycol Monoethyl ether but still it does not work.
> Can I ask for help if anyone knows what was going on and what would be the
> best way to remove this plastic from my section?
>
> Just to give you my embedding solution was MMA -94% , dibutly phthalate-
> 5% and perkadox 16- 0.5%. This have been my solution for years and I do not
> have any problem with the removal of plastic section until now. I was
> thinking that my MMA (M55909) different lot number from Sigma Aldrich may
> have cause this because even I tried to use the MMA to dissolve my plastic
> it does not work.
>
>
> Thank you for and any opinions or protocols are greatly appreciated.
>
>
> Reuel Cornelia
>
> TSRH
>
> 214-559-7766
>
>
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Re: [Histonet] processing Dosophilla flies

[Hobbs, Carl via Histonet]

Hi
Processing them , how?

To Pwax?
To snapfreeze?
To resin?

Kind regards

Carl


  
 
Carl Hobbs FIBMS 
Histology and Imaging Manager 
Wolfson CARD 
Guys Campus, London Bridge  
Kings College London 
London 
SE1 1UL 
  
020 7848 6813
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[Histonet] (no subject)

[seaduster--- via Histonet]

Hey Histonet https://goo.gl/pmPPmg   
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