[Histonet] FW: ER/PR question
This paper might be useful: Ehinger, A., Bendahl, P. O., Rydén, L., Fernö, M., & Alkner, S. (2018). Stability of oestrogen and progesterone receptor antigenicity in formalin‐fixed paraffin‐embedded breast cancer tissue over time. Apmis, 126(9), 746-754. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal Scientist, the Children’s Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -Original Message- From: Heckford, Karen - SMMC-SF via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Friday, 8 February 2019 12:19 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] ER/PR question Good Morning, One of my Pathologists wants me to do some ER/PR's on 9 year old tissue blocks. I know ER and PR can be sensitive are the IHC's going to work or will there be too much antigen decay? Thanks, Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckf...@dignityhealth.org Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Cut-resistant gloves and SOP for frozen brain
Hello - I would appreciate feedback from the HistoNet community on best practices for handling frozen human (or macaque) brain for autoradiography in a research lab. I'm also curious about lightweight cut resistant gloves, and whether they are practical for anything more than changing blades (if even that). We recently obtained frozen slabs of brain tissue that are larger than the material our group routinely sections. The PI purchased a Mar-Med Bone saw to cut the slabs into smaller blocks we can then section and mount on glass slides. It should be a safe option for biologists with no experience using any power tools. I need to update our EH and biosafety protocol for the saw, so I'm also revisiting our BBP/cryostat safety training. The institutional biosafety office suggested lightweight cut-resistant gloves made with ultrahigh molecular weight polyethylene fiber, layered with nitrile. They look like cotton liners, Spec-Tec and Spectra fiber are trade names. Are they useful? If they weren't so expensive (and we didn't need two different sizes), I wouldn't hesitate to try them. We follow standard universal/microbial practices for biohazards, with surgical mask and eye protection for splash hazards when dust/aerosols are a concern. The new Mar-Med saw will go in a fume hood. I've explained the use of push blocks or a jig to hold the tissue when using a band saw (probably have some made from melamine). Any other tips/tricks when using a saw on frozen brain? The company rep suggested ultra-fine blades, but I don't know if diamond would be better for brain. Should brain tissue be cut at -80 degrees or at -20 degrees? Do users chill the saw with ice or dry ice? Cleaning and disinfection for the saw will follow manufacturers SOP for disassembly of the parts and our cryotome disinfection protocol. The cryotome is cleaned and decontaminated at room temperature. Equipment surfaces are sometimes covered with foil to facilitate cleanup. We use lab tissues to clean surfaces and non-removable parts after discarding the blade. (Any foil is also discarded.) Chucks and other parts are removed and soaked in a neutral lab detergent. We clean surfaces with soap, then warm water rinse, then 70% ethanol, then 95% alcohol to remove water. After it is dry, final decontamination is with a waterless EPA-registered spray disinfectant (kills TB); spray it on so that it looks wet for ~2 minutes. 20 minutes after spraying with the EPA-registered disinfected, the cryotome can be set to standard temperature for use. Am I missing anything? We are an academic/research lab, so don't need to follow CAP. Much of our work uses rodent tissue, so there isn't a set schedule for cryotome disinfection - or even cleaning. Lab Specific Training is clean/disinfect both before and after working with potentially infectious material (human or macaque). Thanks, Lynne Jones Research Lab Supervisor Radiochemistry Research Group - Lab Manager Mallinckrodt Institute of Radiology Washington University School of Medicine St Louis, MO CONFIDENTIALITY NOTICE The materials in this message are private and may contain Protected Healthcare Information or other information of a sensitive nature. If you are not the intended recipient, be advised that any unauthorized use, disclosure, copying or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this email in error, please immediately notify the sender via telephone or return mail, and please delete the e-mail without further review, disclosure or copying. The materials in this message are private and may contain Protected Healthcare Information or other information of a sensitive nature. If you are not the intended recipient, be advised that any unauthorized use, disclosure, copying or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this email in error, please immediately notify the sender via telephone or return mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Histonet Digest, Vol 183, Issue 6
To whom it concerns My company for 30 years have sold refurbished vip tissue processor The refurbishment was not done properly your ceramics Value must have small cracks in it allowing chemical to creep into other chemicals on process If u bought this vip from a reputable service company it should be under warranty IMEB Mangement John O’Brien > On Feb 7, 2019, at 11:00 AM, histonet-requ...@lists.utsouthwestern.edu wrote: > > Send Histonet mailing list submissions to >histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to >histonet-requ...@lists.utsouthwestern.edu > > You can reach the person managing the list at >histonet-ow...@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. ER/PR question (Heckford, Karen - SMMC-SF) > 2. Histotech Job in New Hyde Park,NY Area Full Time/Permanent > (Melissa Owens) > 3. Re: ER/PR question (Rene J Buesa) > 4. bachelors of science (Piche, Jessica) > 5. Fwd: VIP 5 (Patti Nelson) > 6. Re: ER/PR question (Allan Wang) > > > -- > > Message: 1 > Date: Thu, 7 Feb 2019 13:19:07 + > From: "Heckford, Karen - SMMC-SF" > To: "histonet@lists.utsouthwestern.edu" > > Subject: [Histonet] ER/PR question > Message-ID: <2d9f0469501a45f8bb9905de59663...@phx-exch-013.chw.edu> > Content-Type: text/plain; charset="us-ascii" > > Good Morning, > One of my Pathologists wants me to do some ER/PR's on 9 year old tissue > blocks. I know ER and PR can be sensitive are the IHC's going to work or > will there be too much antigen decay? > > Thanks, > > Karen Heckford HT ASCP CE > Lead Histology Technician > St. Mary's Medical Center > 450 Stanyan St. > San Francisco, Ca. 94117 > 415-668-1000 ext. 6167 > karen.heckf...@dignityhealth.org > > Caution: This email message, including all content and attachments, is > CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The > information contained in this email message is intended only for the use of > the recipient(s) named above. If the reader of this message is not the > intended recipient or an agent responsible for delivering it to the intended > recipient, you have received this document in error. Any further review, > dissemination, distribution, or copying of this message is strictly > prohibited. If you have received this communication in error, please notify > us immediately by reply email. Thank you > > > > -- > > Message: 2 > Date: Thu, 7 Feb 2019 14:28:39 + > From: Melissa Owens > To: "histonet@lists.utsouthwestern.edu" > > Subject: [Histonet] Histotech Job in New Hyde Park,NY Area Full >Time/Permanent > Message-ID: > > > > Content-Type: text/plain; charset="us-ascii" > > Good Morning, > > Quick note to spread the word about my Full Time Permanent Histotech job in > New Hyde Park, NY area. Please contact me for job description/shift/details. > Thank you, > > Melissa Owens, CHP (ASA) > President, Laboratory Staffing > Allied Search Partners > Direct Line: 407.413.9117 > > > > > > -- > > Message: 3 > Date: Thu, 7 Feb 2019 15:22:35 + (UTC) > From: Rene J Buesa > To: "Heckford, Karen - SMMC-SF" , >"histonet@lists.utsouthwestern.edu" > > Subject: Re: [Histonet] ER/PR question > Message-ID: <1011043176.4635450.1549552955...@mail.yahoo.com> > Content-Type: text/plain; charset=UTF-8 > > Time alone is not the only cause for antigen decay for?it is also associated > with storage conditions and, as such, you cannot "predict" % decay based on > time alone.Just try one block and let the pathologist decide based on the > usefulness of what he sees in that try test.Ren? > >On Thursday, February 7, 2019 8:23 AM, "Heckford, Karen - SMMC-SF via > Histonet" wrote: > > > Good Morning, > One of my Pathologists wants me to do some ER/PR's on 9 year old tissue > blocks.? I know ER and PR can be sensitive are the IHC's going to work or > will there be too much antigen decay? > > Thanks, > > Karen Heckford HT ASCP CE > Lead Histology Technician > St. Mary's Medical Center > 450 Stanyan St. > San Francisco, Ca. 94117 > 415-668-1000 ext. 6167 > karen.heckf...@dignityhealth.org > > Caution:? This email message, including all content and attachments, is > CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED.? The > information contained in this email message is intended only for the use of > the recipient(s) named above. If the reader of this message is not the > intended recipient or an agent responsible for delivering it to the intended > recipient, you have received this document in error.? Any
Re: [Histonet] ER/PR question
Karen Heckford HT ASCP CE at St. Mary's Medical Center in San Francisco, CA asks: >>One of my pathologists wants me to do some ER/PR's on 9 year old tissue blocks. I know ER and PR can be sensitive are the IHC's going to work or will there be too much antigen decay?<< You can only try. There should be some antigen positive ducts and what have you in these 9 year old tissues that should serve as an internal control. If there's nothing positive, I'd interpret negative results cautiously. But it's worth a try. Bob Richmond Samurai Pathologist Maryville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] ER/PR question
The antigen doesn't decay much inside the tissue block, as long as the sections were freshly done. We redo ER/PR on archived samples from 15 years ago and they still stain very strongly. Allan On Thu, Feb 7, 2019 at 8:26 AM Heckford, Karen - SMMC-SF via Histonet < histonet@lists.utsouthwestern.edu> wrote: > Good Morning, > One of my Pathologists wants me to do some ER/PR's on 9 year old tissue > blocks. I know ER and PR can be sensitive are the IHC's going to work or > will there be too much antigen decay? > > Thanks, > > Karen Heckford HT ASCP CE > Lead Histology Technician > St. Mary's Medical Center > 450 Stanyan St. > San Francisco, Ca. 94117 > 415-668-1000 ext. 6167 > karen.heckf...@dignityhealth.org > > Caution: This email message, including all content and attachments, is > CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The > information contained in this email message is intended only for the use of > the recipient(s) named above. If the reader of this message is not the > intended recipient or an agent responsible for delivering it to the > intended recipient, you have received this document in error. Any further > review, dissemination, distribution, or copying of this message is strictly > prohibited. If you have received this communication in error, please > notify us immediately by reply email. Thank you > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Fwd: VIP 5
Patti Nelson HT (ASCP) 909-841-9761 Sent from my iPhone Begin forwarded message: > From: Patricia Nelson > Date: February 7, 2019 at 8:38:23 AM PST > To: "nelsonr...@verizon.net" > Subject: FW: VIP 5 > > > From: Patricia Nelson > Sent: Thursday, February 07, 2019 8:37 AM > To: histonet@lists.utsouthwestern.edu > Subject: VIP 5 > > Hi Histo World, > > HELP!!Mid December our VIP 5 went down. I work in a GI lab with a > workload of 300 daily. Our lab obtained another refurbished VIP 5. But now we > are starting to see the same symptoms as we seen on our old VIP before it > went down. Some of the symptoms are formalin carry over (formalin level goes > down after each run), our 95 w/eosin is carrying over to all solutions all > the way to the paraffin. Now our small GI bx are starting to feel unfixed and > has patchy staining. Has anyone dealt with any of these issues? > We maintenance our VIP weekly and change all solutions and all paraffins Once > a month we do our warm water flush. Below is our set up > > 1st level: Formalin, Formalin, 70%, 80%, 95% w/eosin, > 2nd level: 100,100, 100, xylene, xylene > 3rd level: cleaning xylene, cleaning alcohol, water, empty, fume con. water > > Patti Nelson (HT ASCP) > United Gastroenterologists > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] bachelors of science
Hello Everyone, Do you need a bachelor's of science to sit for the HTL exam? I thought you had to have a bachelor's degree, and the right amount of credits in science, chemistry, etc. Thanks in advance. Jessica Piche, HT(ASCP) Waterbury Hospital ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] ER/PR question
Time alone is not the only cause for antigen decay for it is also associated with storage conditions and, as such, you cannot "predict" % decay based on time alone.Just try one block and let the pathologist decide based on the usefulness of what he sees in that try test.René On Thursday, February 7, 2019 8:23 AM, "Heckford, Karen - SMMC-SF via Histonet" wrote: Good Morning, One of my Pathologists wants me to do some ER/PR's on 9 year old tissue blocks. I know ER and PR can be sensitive are the IHC's going to work or will there be too much antigen decay? Thanks, Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckf...@dignityhealth.org Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Histotech Job in New Hyde Park, NY Area Full Time/Permanent
Good Morning, Quick note to spread the word about my Full Time Permanent Histotech job in New Hyde Park, NY area. Please contact me for job description/shift/details. Thank you, Melissa Owens, CHP (ASA) President, Laboratory Staffing Allied Search Partners Direct Line: 407.413.9117 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] ER/PR question
Good Morning, One of my Pathologists wants me to do some ER/PR's on 9 year old tissue blocks. I know ER and PR can be sensitive are the IHC's going to work or will there be too much antigen decay? Thanks, Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckf...@dignityhealth.org Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
