[Histonet] mouse pituitary

[Atoska Gentry via Histonet]

Hello,

does anyone have a tried & proven method and/or pointers  for mounting mouse 
pituitary for cryo sectioning which guarantees great cross sections?

Thanks!

~Atoska

Sent from Mail for Windows 10

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[Histonet] HT Opening

[Arrington, Karla A via Histonet]

Hello All,

I hope everyone is enjoying summer.

In beautiful Alaska, our hospital caters to the Alaskan Native population with 
their entire medical needs being met.
Currently we have a Histology Technician position available in our pathology 
department. This position is M-F, no weekends and a rotating on-call schedule.
This position comes with excellent benefits and a team work environment. This 
also offers relocation assistance for the correct candidate. Must be ASCP 
certified or eligible.

For immediate consideration, email or FAX a current resume to 
kaarring...@anthc.org and it will be forwarded to 
our hiring manager. Also visit our website at anthc.org and select Careers to 
apply online.

If you have questions regarding this position or how to submit an online 
application, please call ANTHC Human Resources at (907) 729-1301 or email 
care...@anthc.org.


Karla Arrington, HT(ASCP) HIT(AHIMA)
Pathology Supervisor
Alaska Native Medical Center
4315 Diplomacy Drive
Anchorage, AK 99508
Tele: 907-729-1810
Fax:  907-729-1226


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Re: [Histonet] Tissue Adherence problem

[Terri Braud via Histonet]

Hi Deanne - 
Hope this will help
For our Ventana Ultra, we use Leica / Surgipath Apex Superior Adhesive slides.
We use de ionized water in our baths at 45C ish, no additives.
We never "double-dip" our slides (meaning that if we mount a control on the top 
of the slide, we turn the slide upside down to pick it up, only immersing the 
slide to pick up the intended tissue)
We pre-bake IHC slides in a hot air blower over at 65C, 10 min for small 
tissues, and 1 hr for large tissues, then load for IHC.

For special stains, we use the same slides, but they go directly on the Sakura 
Prisma stainer into the instrument oven at 65C for 15 minutes before staining
For H, we use the plain Surgipath Snowcoat  slides, no adhesive, directly on 
the Sakura Prisma stainer into the instrument oven at 65C for 15 minutes before 
staining.  They dry vertically in a rack as we cut, then we load as soon as the 
rack is full.

We once had an issue with tissue falling off and were able to trace it to a new 
tech's liberal use of Paraguard Spray. Stopped using it, wiped everything down 
with alcohol and our problem went away.
Also, many years ago, I had a problem with a bad lot of slides, but these were 
Cardinal's.
I sincerely hope you can get this straightened out.  I know how frustrating 
such a problem can be.
Terri

Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Laboratory
Holy Redeemer Hospital
1648 Huntingdon Pike
Meadowbrook, PA 19046
ph: 215-938-3689
fax: 215-938-3874
Care, Comfort, and Heal

Today's Topics:
   2. Tissue Adherence Issue (Knutson, Deanne)
Message: 2
Date: Wed, 17 Jul 2019 09:21:49 -0500
From: "Knutson, Deanne" 
Subject: [Histonet] Tissue Adherence Issue
Fellow Histonetters -

I would appreciate your feedback on an intermittent issue that has shown up in 
our lab.
All of a sudden, we are having difficulty with tissue specimens falling off of 
our slides on the IHC stains and special stains sporadically.
About a year ago, we switched instruments on the IHC bench from the Leica BOND 
to the ROCHE ULTRA, and we use the Ventana NexES special stainer.
No adherence issues with all of the validation slides that were run.
We have tried various types of slides recently as well, and the issue still 
prevails.

Would any of you mind telling me your slide workflow - type of slide used, 
gelatin or not used in flotation bath, how long slides are cooked, etc for 
your IHC slides, for your special stains slides, and even for your H slides.
I would welcome your suggestions and feedback.

Thank you so much!!!
Deanne Knutson
Supervisor
Anatomic Pathology
"Let All Be Received as Christ."


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Re: [Histonet] Tissue Adherence Issue

[Dilts,Andrew via Histonet]

We find that the amount of time slides spend baking in the oven prior to 
deparaffinization on our Benchmark Ultras tends to make a difference.  Harder 
tissue like brain and bone can even benefit from  2 hours at 58*C.



Andrew Dilts  HTL(ASCP)
Histo Supervisor, Laboratory Services
Phone: (417) 269-5021
andrew.di...@coxhealth.com
www.coxhealth.com



-Original Message-
From: Knutson, Deanne 
Sent: Wednesday, July 17, 2019 9:22 AM
To: 'histonet@lists.utsouthwestern.edu' 
Subject: [Histonet] Tissue Adherence Issue

Fellow Histonetters -

I would appreciate your feedback on an intermittent issue that has shown up in 
our lab.
All of a sudden, we are having difficulty with tissue specimens falling off of 
our slides on the IHC stains and special stains sporadically.
About a year ago, we switched instruments on the IHC bench from the Leica BOND 
to the ROCHE ULTRA, and we use the Ventana NexES special stainer.
No adherence issues with all of the validation slides that were run.
We have tried various types of slides recently as well, and the issue still 
prevails.

Would any of you mind telling me your slide workflow - type of slide used, 
gelatin or not used in flotation bath, how long slides are cooked, etc for 
your IHC slides, for your special stains slides, and even for your H slides.
I would welcome your suggestions and feedback.

Thank you so much!!!

Deanne Knutson
Supervisor
Anatomic Pathology

 [X]
 "Let All Be Received as Christ."



  
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[Histonet] Histology Opportunities available in Atlanta and Columbus GA.

[Pam Barker via Histonet]

Hello Histonetters,
I hope you are having a great week!
I have a couple of new positions to tell you about – 
Both Opportunities are RELIA EXCLUSIVES!!
These are permanent full time day shift positions offering excellent
compensation and benefits.
IHC Specialist – Atlanta, GA
Histotech – Columbus, GA
If you or anyone you know might be interested, please contact me.
Histonetters, If I place someone you refer You will earn a referral fee!
I can be reached toll free at the office at 866-607-3542 or
rel...@earthlink.net or you can always catch me on cell via call or text at
407-353-5070. 

Thanks-Pam

#jobs4myhistopeeps 
#ilovemyhistopeeps
#histopeeps
Follow my hashtags and make your day great and your career greater!!

Right Place, Right Time, Right Move with RELIA!

Thank You!
 Pam M. Barker 
Pam Barker
President/Senior Recruiting Specialist-Histology
RELIA Solutions
Specialists in Allied Healthcare Recruiting
5703 Red Bug Lake Road #330
Winter Springs, FL 32708-4969
Phone: (407)657-2027
Cell: (407)353-5070
FAX: (407)678-2788
E-mail: rel...@earthlink.net 
https://www.facebook.com/RELIASolutionsforhistologyprofessionals
www.facebook.com/PamBarkerRELIA
www.linkedin.com/in/reliasolutions
www.twitter.com/pamatrelia 



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[Histonet] Tissue Adherence Issue

[Knutson, Deanne via Histonet]

Fellow Histonetters -

I would appreciate your feedback on an intermittent issue that has shown up in 
our lab.
All of a sudden, we are having difficulty with tissue specimens falling off of 
our slides on the IHC stains and special stains sporadically.
About a year ago, we switched instruments on the IHC bench from the Leica BOND 
to the ROCHE ULTRA, and we use the Ventana NexES special stainer.
No adherence issues with all of the validation slides that were run.
We have tried various types of slides recently as well, and the issue still 
prevails.

Would any of you mind telling me your slide workflow - type of slide used, 
gelatin or not used in flotation bath, how long slides are cooked, etc for 
your IHC slides, for your special stains slides, and even for your H slides.
I would welcome your suggestions and feedback.

Thank you so much!!!

Deanne Knutson
Supervisor
Anatomic Pathology

 [X]
 "Let All Be Received as Christ."



  
This email may include confidential and privileged information. If this is not 
intended for your use, please destroy immediately and contact the sender of the 
message.

This email and attachments contain information that may be confidential or 
privileged. If you are not the intended recipient, notify the sender at once 
and delete this message completely from your information system. Further use, 
disclosure, or copying of information contained in this email is not 
authorized, and any such action should not be construed as a waiver of 
privilege or other confidentiality protections.
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[Histonet] Job

[shultz11 via Histonet]

Histology job a t Louisiana State University in Baton Rouge, LA. Must have IHC 
experience.Thanks. Kendra Shultz225-578-9724Sent from my Verizon, Samsung 
Galaxy smartphone
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