[Histonet] Our Day!
Happy Histotechnology Professionals Day to All my Colleagues! Did you know it is also Dezna Sheehan's Birthday too? I am honored to have known her. What an incredible mentor. Akemi Allison BS, HT/HTL (ASCP) Pathology Manager Monterey Bay GI Consultants Laboratory 23 Upper Ragsdale Drive, Suite 200 Monterey, CA 93940 Email: aalli...@montereygi.com Tele: (831) 375-3577 X117 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Mineral Oil in Microwave Processing.
I have a Milestone Microwave Tissue Processor and use Prowave from Milestone. It’s proprietary, but it has alcohol in it and does a great job on our tissue.I haven’t used mineral oil for years! Akemi Allison HT/HTL (ASCP) Pathology Manager Monterey Bay GI Consultants Laboratory 23 Upper Ragsdale Drive, Suite 200 Monterey, CA 93940 Email: aalli...@montereygi.com (831) 375-3577 X117 On Feb 16, 2014, at 6:44 PM, Cesar Francisco Romero chesar...@hotmail.com wrote: Dear People from Histonet Do you still use Mineral Oil in your Microwave Processing Protocol ? Or you only use ethyl alcohol ~ Isopropyl alcohol and Paraffin . I will apreciate any advice. Thank you all Cesar Romero Buenos Aires Argentina ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Trade H-Pylori for Fungus Control
Hi everyone in histo land! I hope you all had a wonderful Valentines Day. I am hoping I might get a Valentines gift of some fungus control tissue. I am getting down to the tail end of mine. I would be more than happy to trade some (+) H Pylori blocks in exchange. I would be more than happy to pay for the shipping charges. Thank you in advance, Akemi Allison, BS, HT/HTL (ASCP) Pathology Manager Monterey Bay GI Consultants Laboratory Email: aalli...@montereygi.com Tele: (831) 375-3577 X117 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Eileen (Akemi) Allison-Tacha wants to stay in touch on LinkedIn
LinkedIn I'd like to add you to my professional network on LinkedIn. - Eileen (Akemi) Allison-Tacha Eileen (Akemi) Allison-Tacha Director at Phoenix Lab Consulting San Francisco Bay Area Confirm that you know Eileen (Akemi) Allison-Tacha https://www.linkedin.com/e/-le17ov-gd1tgzoc-70/isd/1580252622/4TPckY5L/ -- (c) 2010, LinkedIn Corporation ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Thermo's Excelsior Tissue Processor Program's
Hi All of you in Histoland, I am working with a facility that recently purchased a Thermo Excelsior Tissue Processor. They have had processing problems with several of their specimens using non-xylene clearing agent. These issues were with both bx's and larger tissues. The program and the non-xylene clearing agent was recommended by the technical staff at Thermo. The histology staff have switched back to using xylene verses the non-xylene clearing agent, and most of the issues have disappeared. Also, the histotech's were using reagent alcohol, histological grade. This grade of alcohol is denatured with methyl alcohol. The sales representative was in and informed us that this type of alcohol has damaging effects on the instrument. The representative will be coming in to flush out the system and reprogram the instrument next Monday. I looked over the current VIP program which is being used, and the program, timing and temperatures are a little different from most hospital and private laboratories I have worked with. We are going to use basically the same program for the Excelsior, that we use on the VIP. I would like to know what other Excelsior Tissue Processor users that use xylene have for their programs. It would be great to compare the reagents, programs, timing, and temperatures for Routine overnight runs and for Rapid Biopsy Runs. Thank you in advance for your assistance. Akemi Allison-Tacha BS, HT(ASCP)HTL Director Phoenix Lab Consulting E-Mail: akemiat3...@yahoo.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Email Spoofed. addresses were highjacked
Hi All, The email below was sent to me in response to the Scam. I guess someone from histonet contacted him regarding the scam, which was spammed. It's interesting to find out how vulnerable we all are. Yahoo's customer support has had high volumes of similar problems. Akemi, Boy! Peter needs an Oxycontin! Maybe more than one. Don't worry about this. The correct term is being Email Spoofed. From my friends, it happens when some computer hijacker either creates content on a new or existing web site that you ended up clicking on. Peter suggests it is Where you visited, but it could be anywhere, even on a legitimate site. Some people I've talked to think Microsoft's web page is loaded with Spoof and Spyware! Sometimes, it is a computer worm or virus affecting your computer. Most of the time, it is associated with your email provider. [Most of my friends with Yahoo email have been Spoofed.] I would run a up-to-date anti-virus software, and (especially if that doesn't help) ask for help from Yahoo to quarantine or fix your address. You probably have it fixed already. If not, it will do it again until someone stops it. It IS only email. So what??? Hugh-Hawaii ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] RE: Akemi - Urgent!
Hi All, I received an e-mail and an early morning call from my friend Helen Hedor this morning stating that she received a e-mail scam from me stating; FISHING FOR MONEY. I did in NO WAY send this e-mail! Please do not in anyway answer or send money to the bogus e-mail scam which was sent in my name. THIS IS SCARY! I don't know what has happened, but I came home last night and my yahoo account sent me an e-mail which stated that my e-mail has been compromised for scam purposes, and if I didn't answer the questions below I would have my account frozen. I immediately replied and answered the questions, but then I thought, what if this is a scam to extract personal information, so I copied the the questionnaire and sent it to the yahoo alert center. I received a reply that they would check into it. This morning I couldn't get into my e-mail account so I had to jump a bunch of hoops and reset my password. I hope none of you have this happen to you. The Yahoo Account warning stated: Yahoo Mail has discovered series of illegal attempts on your Yahoo Account from bad Ip Location and will shut your account as it has been flagged as a spam account. Filling Correct Information Carefully and Sending to Yahoo Alert Center: I hope this was legitimate! The information they wanted was my user name, password and birthday. Sincerely,Akemi Allison-Tacha BS, HT(ASCP)HTL E-Mail: akemiat3...@yahoo.com --- On Thu, 11/12/09, Helen Fedor hfe...@jhmi.edu wrote: From: Helen Fedor hfe...@jhmi.edu Subject: [Histonet] RE: Akemi - Urgent! To: Breeden, Sara sbree...@nmda.nmsu.edu, histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Date: Thursday, November 12, 2009, 6:29 AM I also receive one of the scam emails. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Thursday, November 12, 2009 9:21 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Akemi - Urgent! Akemi Allison-Tacha - email me immediately, please. I think you are being scammed and I don't want to email you directly. Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] RE: This is what was sent Akemi - Urgent!
This is the Yahoo ALERT that was sent it looks legitimate;From: basant...@yahoo.com Subject:Dear Valued Member, Date: November 11, 2009 10:49:15 AM PST To: undisclosed recipients: ; Reply-To: messagecenter2...@live.comaccount Alert Dear Valued Member, Yahoo Mail has discovered series of illegal attempts on your Yahoo Account from bad Ip Location and will shut your account as it has been flagged as a spam account. You are the thus immediately required to Secure Your Online Access by Manually Filling The Form Below by clicking on the reply-to button on your page, Filling Correct Information Carefully and Sending to Yahoo Alert Center: UserName: ...Password: ...Date of Birth: ..Country Or Territory : ... After following the instructions in the sheet, your account will not be interrupted and will continue as normal. Thanks for your attention to this request. We apologize for any inconvenience. Warning!!! Account owner that refuses to update his or her account before two weeks of receiving this warning will lose his or her account permanently. Akemi Allison-Tacha BS, HT(ASCP)HTL PresidentPhoenix Lab ConsultingE-Mail: akemiat3...@yahoo.com --- On Thu, 11/12/09, Adam . anonwu...@gmail.com wrote: From: Adam . anonwu...@gmail.com Subject: Re: [Histonet] RE: Akemi - Urgent! To: histonet@lists.utsouthwestern.edu Date: Thursday, November 12, 2009, 9:13 AM Phishing also happens over the phone as well. It is not unheard of that people get phone calls claiming to be from a bank asking for personal information (why would a bank need your account number... aren't they your bank and don't they already have this information?). It is generally a very bad idea to give any personal information out in any venue other than the physical office of the institution which you do business. If the company suspects that your account has been compromised, they will disable it and then ask you to contact them. If you receive such an e-mail, do not click on any links in it or dial any phone number contained within. Instead, look up the company's contact information (in a phone book or via a search engine) and use the publicly available contact information. Adam On Thu, Nov 12, 2009 at 11:04 AM, Rene J Buesa rjbu...@yahoo.com wrote: Akemi: And that was your second mistake. Why the server, in this case Yahoo, would like your password and your birth day when that is not originally needed? I NEVER EVER answer anything even if I am told that my life depends on it. Never answer a request for information of any kind. If it a legitimate request they will call you on the phone. René J. --- On Thu, 11/12/09, Akemi Allison-Tacha akemiat3...@yahoo.com wrote: From: Akemi Allison-Tacha akemiat3...@yahoo.com Subject: Re: [Histonet] RE: Akemi - Urgent! To: SaraBreeden sbree...@nmda.nmsu.edu, histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu, Helen Fedor hfe...@jhmi.edu Date: Thursday, November 12, 2009, 11:03 AM Hi All, I received an e-mail and an early morning call from my friend Helen Hedor this morning stating that she received a e-mail scam from me stating; FISHING FOR MONEY. I did in NO WAY send this e-mail! Please do not in anyway answer or send money to the bogus e-mail scam which was sent in my name.. THIS IS SCARY! I don't know what has happened, but I came home last night and my yahoo account sent me an e-mail which stated that my e-mail has been compromised for scam purposes, and if I didn't answer the questions below I would have my account frozen. I immediately replied and answered the questions, but then I thought, what if this is a scam to extract personal information, so I copied the the questionnaire and sent it to the yahoo alert center. I received a reply that they would check into it. This morning I couldn't get into my e-mail account so I had to jump a bunch of hoops and reset my password. I hope none of you have this happen to you. The Yahoo Account warning stated: Yahoo Mail has discovered series of illegal attempts on your Yahoo Account from bad Ip Location and will shut your account as it has been flagged as a spam account. Filling Correct Information Carefully and Sending to Yahoo Alert Center: I hope this was legitimate! The information they wanted was my user name, password and birthday. Sincerely,Akemi Allison-Tacha BS, HT(ASCP)HTL E-Mail: akemiat3...@yahoo.com --- On Thu, 11/12/09, Helen Fedor hfe...@jhmi.edu wrote: From: Helen Fedor hfe...@jhmi.edu Subject: [Histonet] RE: Akemi - Urgent! To: Breeden, Sara sbree...@nmda.nmsu.edu, histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Date: Thursday, November 12, 2009, 6:29 AM I also receive one of the scam emails. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto: histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Breeden
Re: [Histonet] RE:Akemi - Urgent! Scammed!
Well Guys, looks like I have definitely been Scammed and Spammed! After I thought about the possible rouse, I did however copy the original Alert that was sent and sent it to the Yahoo Alert Center. The REAL Yahoo Alert Center has the CRIMINAL'S e-mail addresses to do their investigation. Akemi Allison-Tacha BS, HT(ASCP)HTL E-Mail: akemiat3...@yahoo.com --- On Thu, 11/12/09, Shawn Leslie shawnles...@embarqmail.com wrote: From: Shawn Leslie shawnles...@embarqmail.com Subject: Re: [Histonet] RE: This is what was sent Akemi - Urgent! To: Akemi Allison-Tacha akemiat3...@yahoo.com Date: Thursday, November 12, 2009, 9:35 AM For one...The return address is a Live address...That's MSN not YahooYou are definitely being SCAMMED... - Original Message - From: Akemi Allison-Tacha akemiat3...@yahoo.com To: histonet@lists.utsouthwestern.edu, Adam . anonwu...@gmail.com Sent: Thursday, November 12, 2009 12:26:02 PM GMT -05:00 US/Canada Eastern Subject: Re: [Histonet] RE: This is what was sent Akemi - Urgent! This is the Yahoo ALERT that was sent it looks legitimate;From: basant...@yahoo.com Subject: Dear Valued Member, Date: November 11, 2009 10:49:15 AM PST To: undisclosed recipients: ; Reply-To: messagecenter2...@live.comaccount Alert Dear Valued Member, Yahoo Mail has discovered series of illegal attempts on your Yahoo Account from bad Ip Location and will shut your account as it has been flagged as a spam account. You are the thus immediately required to Secure Your Online Access by Manually Filling The Form Below by clicking on the reply-to button on your page, Filling Correct Information Carefully and Sending to Yahoo Alert Center: UserName: ...Password: ...Date of Birth: ..Country Or Territory : ... After following the instructions in the sheet, your account will not be interrupted and will continue as normal. Thanks for your attention to this request. We apologize for any inconvenience. Warning!!! Account owner that refuses to update his or her account before two weeks of receiving this warning will lose his or her account permanently. Akemi Allison-Tacha BS, HT(ASCP)HTL PresidentPhoenix Lab ConsultingE-Mail: akemiat3...@yahoo.com --- On Thu, 11/12/09, Adam . anonwu...@gmail.com wrote: From: Adam . anonwu...@gmail.com Subject: Re: [Histonet] RE: Akemi - Urgent! To: histonet@lists.utsouthwestern.edu Date: Thursday, November 12, 2009, 9:13 AM Phishing also happens over the phone as well. It is not unheard of that people get phone calls claiming to be from a bank asking for personal information (why would a bank need your account number... aren't they your bank and don't they already have this information?). It is generally a very bad idea to give any personal information out in any venue other than the physical office of the institution which you do business. If the company suspects that your account has been compromised, they will disable it and then ask you to contact them. If you receive such an e-mail, do not click on any links in it or dial any phone number contained within. Instead, look up the company's contact information (in a phone book or via a search engine) and use the publicly available contact information. Adam On Thu, Nov 12, 2009 at 11:04 AM, Rene J Buesa rjbu...@yahoo.com wrote: Akemi: And that was your second mistake. Why the server, in this case Yahoo, would like your password and your birth day when that is not originally needed? I NEVER EVER answer anything even if I am told that my life depends on it. Never answer a request for information of any kind. If it a legitimate request they will call you on the phone. René J. --- On Thu, 11/12/09, Akemi Allison-Tacha akemiat3...@yahoo.com wrote: From: Akemi Allison-Tacha akemiat3...@yahoo.com Subject: Re: [Histonet] RE: Akemi - Urgent! To: SaraBreeden sbree...@nmda.nmsu.edu, histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu, Helen Fedor hfe...@jhmi.edu Date: Thursday, November 12, 2009, 11:03 AM Hi All, I received an e-mail and an early morning call from my friend Helen Hedor this morning stating that she received a e-mail scam from me stating; FISHING FOR MONEY. I did in NO WAY send this e-mail! Please do not in anyway answer or send money to the bogus e-mail scam which was sent in my name.. THIS IS SCARY! I don't know what has happened, but I came home last night and my yahoo account sent me an e-mail which stated that my e-mail has been compromised for scam purposes, and if I didn't answer the questions below I would have my account frozen. I immediately replied and answered the questions, but then I thought, what if this is a scam to extract personal information, so I copied the the questionnaire and sent it to the yahoo alert center. I received a reply that they would check into it. This morning I couldn't get into my e-mail account so I had to jump
Re: [Histonet] RE: Akemi - Response to Vinnie
Hi Vinnie and anyone else who is in my address book, I am so sorry! This has become a nightmare. I have tried to contact everyone, but I must have at least close to 300 people in my address book. Akemi --- On Thu, 11/12/09, Della Speranza, Vinnie del...@musc.edu wrote: From: Della Speranza, Vinnie del...@musc.edu Subject: [Histonet] RE: Akemi - Urgent! To: 'Helen Fedor' hfe...@jhmi.edu, Breeden, Sara sbree...@nmda.nmsu.edu, histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Date: Thursday, November 12, 2009, 3:30 PM I also received the scam message purportedly from Akemi so something is amiss. I can only assume Akemi that your address book/contact list has been acquired by whoever is behind this. Vinnie Della Speranza Manager for Anatomic Pathology Services 165 Ashley Avenue Suite 309 Charleston, SC 29425 tel. 843-792-6353 fax. 843-792-8974 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Helen Fedor Sent: Thursday, November 12, 2009 9:30 AM To: Breeden, Sara; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Akemi - Urgent! I also receive one of the scam emails. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Thursday, November 12, 2009 9:21 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Akemi - Urgent! Akemi Allison-Tacha - email me immediately, please. I think you are being scammed and I don't want to email you directly. Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] new antibody validations
Hi Sharon, This was discussed around 1 1/2 months ago in great detail. Check the histonet archives for antibody validation. Akemi Allison-Tacha BS, HT (ASCP) HTL President/Director Phoenix Lab Consulting Tele: 408.402.5257 Cell: 408.335.9994 E-Mail: akemiat3...@yahoo.com On Oct 29, 2009, at 10:31 AM, Campbell, Sharon wrote: Hello Histonet! We are going to be optimizing and validating new antibodies. What is the recommended number of cases for validating once we have optimized? I have heard 20 and also 40 cases. Also, How is a negative control to be optimized and validated? Thank you. Sharon Campbell, HTL(ASCP)CM, BSBM Histology Supervisor Celligent Diagnostics, LLC 101 East W.T. Harris Blvd, Suite 1212 Charlotte, NC 28262 800-524-6779 ext. 104 704-970-3304 Direct Line scampb...@celligent.net ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] NSH Website down again
Hi out there, The NSH website is down again. I tried this weekend, as well as this morning and this is what comes-up. Warning: file(http://www.e-guana.net/organizations.php3?action=printContentItemorgid=111typeID=1234itemID=19243) [function.file]: failed to open stream: HTTP request failed! HTTP/1.1 404 Not Found in /home/nsh/nsh-www/organizations.php3 on line 23 Warning: implode() [function.implode]: Bad arguments. in /home/nsh/nsh-www/organizations.php3 on line 23 Akemi Allison-Tacha BS, HT(ASCP)HTL PresidentPhoenix Lab ConsultingTele: 408.402.5257 Cell: 408.335.9994 E-Mail: akemiat3...@yahoo.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] RE:Missing the point of Plants-in-the-lab OT
Hi All, I think all of you are missing the point of Patti's question. She stated that her lab was dinged for having plants in the lab by a CAP inspector. I had the same thing happen to me years ago. The inspector stated that plants attract insects that can contaminate a supposedly clean environment. Patti has an extremely well organized lab that only had a small phase (1) deficiency last year. I think the inspector couldn't find anything, so they had to come up with this ridiculous infraction. Akemi Allison-Tacha BS, HT(ASCP)HTL PresidentPhoenix Lab ConsultingTele: 408.402.5257 Cell: 408.335.9994 E-Mail: akemiat3...@yahoo.com --- On Thu, 10/22/09, Blazek, Linda lbla...@digestivespecialists.com wrote: From: Blazek, Linda lbla...@digestivespecialists.com Subject: [Histonet] RE: Plants-in-the-lab OT To: 'Breeden, Sara' sbree...@nmda.nmsu.edu, histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Date: Thursday, October 22, 2009, 12:15 PM I don't know Sally, but where I worked many moons ago I had a spider plant that was extremely prolific. The powers that be made me remove it from the lab for an inspection. It went to live in one of the administrator's office for several months. And died! I think it needed the fumes! Or it missed me. Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton Phone: (937) 293-4424 ext 7118 Email: lbla...@digestivespecialists.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Thursday, October 22, 2009 3:02 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Plants-in-the-lab OT I think it's the fluorescent lights that makes them thrive. The absorption of Fume Matter is a secondary, but beneficial, effect. You go, chlorophyll! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Plants in lab
Well, that makes more sense, especially after Mary's statement Some Health departments state that plants and their potting soil can be a potential microbial and fungi contaminate in the lab. Mary Anne Haynes Patti, you do have a wonderful assortment of plants lining your window sill.. Akemi Allison-Tacha BS, HT(ASCP)HTL PresidentPhoenix Lab ConsultingTele: 408.402.5257 Cell: 408.335.9994 E-Mail: akemiat3...@yahoo.com --- On Thu, 10/22/09, Patti Loykasek ployka...@phenopath.com wrote: From: Patti Loykasek ployka...@phenopath.com Subject: [Histonet] Plants in lab To: histonet histo...@pathology.swmed.edu Date: Thursday, October 22, 2009, 12:44 PM Hi All. Just to clarify - the inspection we had was not a CAP inspection.. It was an audit based on the GLP regulations. Thanks for all the feedback on plants in the lab. Patti Ann Loykasek PhenoPath Laboratories This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A.. at (206) 374-9000. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] NSH Website down
Hi, I just tried to get into the NSH website and it's down. Akemi Allison-Tacha BS, HT(ASCP)HTL PresidentPhoenix Lab ConsultingTele: 408.402.5257 Cell: 408.335.9994 E-Mail: akemiat3...@yahoo.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] OSCAR control
Dr. Allen Gown, from PhenoPath laboratories developed this antibody. Perhaps you might contact Patti Loykasek at PhenoPath at (206) 374-9000 and she might help you. Akemi Allison-Tacha BS, HT(ASCP)HTL Histology Manager APMG Laboratories 105A Cooper Court, Los Gatos, CA 95032 Contact: 800.848.2764 V/M: 408.884.2718 Fax: 408.884.2758 Cell: 408.335.9994 (W) E-Mail: aallison-ta...@apmglab.com (P) E-Mail: akemiat3...@yahoo.com --- On Mon, 9/28/09, Maria Katleba maria.katl...@stjoe.org wrote: From: Maria Katleba maria.katl...@stjoe.org Subject: [Histonet] OSCAR control To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Date: Monday, September 28, 2009, 11:57 AM Any ideas on what I can use to optimize OSACR antibody? Maria Katleba HT(ASCP), MS Pathology Dept. Mgr. Queen of the Valley Medical Center 707-294-9229 cell 707-252-4411 x3689 Notice from St. Joseph Health System: Please note that the information contained in this message may be privileged and confidential and protected from disclosure. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Fw: Re: [Histonet] antibody validation Thanks, but need Non-U also
Hi Cynthia, Thank you, this is exactly what I was looking for. You are a rare jewel. Especially for the size of your facility. I am impressed! I wish I would have received more feed-back from small labs, but that's OK. I have been doing some pro-active sleuthing of local labs. After numerous conversations with my fellow histologists that do IHC, I am finding out what I have long suspected and have experienced while doing technical support and consulting. The routine histology laboratory is multitasking all of their regular histology duties with the addition of IHC testing. Pathologists do not want to lose the IHC revenue to a reference laboratory, so they bring on-board simple IHC panels, and send out their esoteric or prognostic markers to a well known reference laboratory. Most histologists have been asked to bring on board IHC, without the proper educational background or tools. The average histologist has not had the opportunity to take continuing education classes, or even go on the histonet. This may be due to lack of continuing education funds, or lack of interest; It is amazing that pathologists can go to their continuing education meetings, but don't support their histologists. Unfortunately, these histologists do not understand the importance of standardization or validation steps. When something goes bump in the night, they are unaware how to troubleshoot the problem. This is unfortunate for the histologist, the pathologist and most of all, the patient. One more reason for pathologists to support continuing education. Hope to see you all at NSH! Akemi Allison-Tacha BS, HT(ASCP)HTL Histology Manager APMG Laboratories 105A Cooper Court, Los Gatos, CA 95032 Contact: 800.848.2764 V/M: 408.884.2718 Fax: 408.884.2758 Cell: 408.335.9994 (W) E-Mail: aallison-ta...@apmglab.com (P) E-Mail: akemiat3...@yahoo.com --- On Mon, 9/14/09, Cynthia Robinson robin...@mercyhealth.com wrote: From: Cynthia Robinson robin...@mercyhealth.com Subject: Re: [Histonet] antibody validation Thanks, but need Non-U also To: Akemi Allison-Tacha akemiat3...@yahoo.com Date: Monday, September 14, 2009, 11:08 AM Hi, I work at 250 bed facility. For the initial protocol workup we use the manufacturer recommended protocol and run an IHC TMA control block with 25 different tissues and tumors. Once the pathologist has looked and responded I search for 5-10 cases with expected positive results and then a selection of 5-10 normal or negative tissues and run the protocol. Hope this is what you were wanting. Cindi Cindi Robinson HT(ASCP) Mercy Medical Center Dunes Medical Laboratories 350 W Anchor Dr Dakota Dunes SD 57049 phone-712-279-2768 robin...@mercyhealth.com Akemi Allison-Tacha akemiat3...@yahoo.com 9/14/2009 12:53 PM Hi All, I would like to thank those of you who have responded to the e-mail below regarding antibody validation. The information I received was pretty much what I expected from university IHC labs. I would like to know what smaller IHC labs are doing for validation and re-validation when changes are made to their protocols. Thank you again for any and all responses, Akemi Akemi Allison-Tacha BS, HT(ASCP)HTL Histology Manager APMG Laboratories 105A Cooper Court, Los Gatos, CA 95032 Contact: 800.848.2764 V/M: 408.884.2718 Fax: 408.884.2758 Cell: 408.335.9994 (W) E-Mail: aallison-ta...@apmglab.com (P) E-Mail: akemiat3...@yahoo.com --- On Fri, 9/11/09, Akemi Allison-Tacha akemiat3...@yahoo.com wrote: From: Akemi Allison-Tacha akemiat3...@yahoo.com Subject: [Histonet] antibody validation To: histonet histonet@lists.utsouthwestern.edu Date: Friday, September 11, 2009, 8:20 AM Good Morning and Happy Friday out there in Histo-Land! I would like your assistance in an issue that I have just become aware of regarding antibody validation. I have been requested to bring on-board approximately (7) new antibodies in the immediate future, and several antibodies later. Although, many of you who know me realize I have worked in IHC RD for a well known IHC company for many years, it has been a while since I have overseen a clinical IHC lab. I would like your assistance and input on how you are validating new antibodies. Not counting Her2-neu, it has been my understanding that it is recommended to test samples with varying antigen expression from 10-15 cases. Furthermore, you should check for Sensitivity: expression range of Positive cases, low to high (10-15 cases). Specificity: (+) vs expected (-) cases/tissues (10-15 cases) and Precision (Reproducibility): It has been my practice that any changes that are made to existing protocols, such as a new detection kit or antigen retrieval methodology, also requires re-validation. I am curious how you approach validation. Also, do any of you just use a single known positive control for that antibody, and run it, and if it is positive, feel that it is satisfactory, and put it on-line for testing. Thank
RE: [Histonet] Special stains and microwave
Hi Rosa, Excuse me if I am wrong, but I think Bill is referring to using a microwave for special stains, and you are referring to a Microwave Tissue Processor. I have used an oven set at a higher temperature (85 -110 degrees C) for staining the silver portion of the GMS, and it generally only takes between 10-15 minutes. That is if you place the silver solution in the oven for approximately 10 minutes prior to use. I also use the oven set at 60-62 degrees C. for the bouins portion of the Trichrome stain. Although using a water bath at 60 degrees C. is the preferred method. You have more control using a oven than using a microwave. With a microwave, results vary with the # of slides placed in the coplin jar, as well as the placement in the microwave. There are variations in microwave intensities if you don't have a carousel. You also may have boil over of solutions. Food for thought. Akemi Akemi Allison-Tacha BS, HT(ASCP)HTL Histology Manager APMG Laboratories 105A Cooper Court, Los Gatos, CA 95032 Contact: 800.848.2764 V/M: 408.884.2718 Fax: 408.884.2758 Cell: 408.335.9994 (W) E-Mail: aallison-ta...@apmglab.com (P) E-Mail: akemiat3...@yahoo.com --- On Thu, 8/27/09, Rosa Fields rfie...@gidocs.net wrote: From: Rosa Fields rfie...@gidocs.net Subject: RE: [Histonet] Special stains and microwave To: O'Donnell, Bill billodonn...@catholichealth.net, histonet@lists.utsouthwestern.edu Date: Thursday, August 27, 2009, 2:26 PM Bill, I can highly recommend the EBS processor microwave. At a base price of 8,900 (last I checked) it is one of the lowest cost processors on the market. I don't know if having the flexibility to process in this new microwave may help justify the price? I purchased mine from Stat Lab, and the customer service from the folks at EBS was outstanding; they even had a prob and air agitator block style holder designed and built for me when I suggested that it should come with one. I really like our model, and it offers great flexibility in our lab. Rosa Fields, HT (ASCP) Gastroenterology Specialties Histology Supervisor 4545 R Street Lincoln, NE 68503 402-465-4545 rfie...@gidocs.net The information contained in the message and the documents accompanying this message contain information that is privileged and confidential and is intended only for the use of the individual or entity named above. If the reader of this message is not the intended recipient or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication, other than its return to the sender, is strictly prohibited. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of O'Donnell, Bill Sent: Thursday, August 27, 2009 4:09 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Special stains and microwave Greetings Histonetters, We have finally come to the point where we need to fish or cut bait in relation to purchasing a laboratory approved microwave and venting it. While I think it would be cool to have one, I'm wondering if the cost is justified to do a handful of special stains that could be done (though more slowly) in a laboratory oven like the one we already have. Anyone with the time or desire to opine, please chime in. Those who have purchased such a microwave, I'll gladly take suggestions. Thanks in advance William (Bill) O'Donnell, HT (ASCP) QIHC Lead Histologist Good Samaritan Hospital 10 East 31st Street Kearney, NE 68847 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Reimbursement for IHC Tests from Medicare/Medicaid
Good Morning Histology Managers, The Chief Medical Director here at my facility proposed a question the other day regarding reimbursement for IHC testing from Medicare /Medicaid patients. I asked our billing manager, and she was going to investigate the matter, but I know my fellow histology managers out there in histoland are pretty up on these matters, so I am asking once again for your expert knowledge. The laws have been going back and forth regarding how much we can charge, and I have been out of the loop for awhile. Can we charge for more than (2) IHC tests for these patients? Let's say you want to do a panel that consists of (5) IHC tests, can we only get reimbursed for (2) of those tests? Any assistance would be greatly appreciated. Thank you in advance for your information, Akemi Akemi Allison-Tacha BS, HT(ASCP)HTL Histology Manager APMG Laboratories 105A Cooper Court, Los Gatos, CA 95032 Contact: 800.848.2764 V/M: 408.884.2718 Fax: 408.884.2758 Cell: 408.335.9994 (W) E-Mail: aallison-ta...@apmglab.com (P) E-Mail: akemiat3...@yahoo.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] NSH Meeting Hotels
Hi All, Anyone trying to reserve a room at the Doubletree should read this. I just tried to reserve a room for the upcoming Symposium. I went to the NSH website and looked under the Hotel Travel icon and saw that the only hotel that wasn't Sold Out was the Double Tree. I called and they never heard of us NSH. I read all the information to the reservation person and she came up with a blank. I requested the supervisor. After searching for about 15 minutes, she found us under histotechnology. Anyone trying to book a room should state that our GROUP CODE is CI0N Akemi Allison-Tacha BS, HT(ASCP)HTL Histology Manager APMG Laboratories 105A Cooper Court, Los Gatos, CA 95032 Contact: 800.848.2764 V/M: 408.884.2718 Fax: 408.884.2758 Cell: 408.335.9994 (W) E-Mail: aallison-ta...@apmglab.com (P) E-Mail: akemiat3...@yahoo.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] General Orientation Checklist
I too am interested in the information. Please send it to me. I tried to send information with an attachment in the past to histonet and it never went through. I guess the only way an attachment can be sent is to an individual. Links are acceptable. Regards,Akemi Akemi Allison-Tacha, BS, HT/HTLE-Mail: akemiat3...@yahoo.com --- On Thu, 7/30/09, Cristi stephenson cls71...@sbcglobal.net wrote: From: Cristi stephenson cls71...@sbcglobal.net Subject: Re: [Histonet] General Orientation Checklist To: Amy Self as...@georgetownhospitalsystem.org, tahs...@brain.net.pk Cc: histonet@lists.utsouthwestern.edu Date: Thursday, July 30, 2009, 8:46 PM Hello, I am sorry, I am also interested in this, but I don't see the attachment? Thanks, Cristi Stephenson MSA, HT(ASCP) --- On Wed, 7/29/09, tahs...@brain.net.pk tahs...@brain.net.pk wrote: From: tahs...@brain.net.pk tahs...@brain.net.pk Subject: Re: [Histonet] General Orientation Checklist To: Amy Self as...@georgetownhospitalsystem.org Cc: histonet@lists.utsouthwestern.edu Date: Wednesday, July 29, 2009, 8:08 AM The file is attached with. M. Tahseen, Supervisor Histopathology SKMCHRC, Lahore, Pakistan Good Day Histonetters, Does anyone have a general orientation checklist for histology for new hires that they would be willing to share with me? Thanks in advance for your help, Amy Amy Self Georgetown Hospital System 843-527-7179 NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -Inline Attachment Follows- ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Paraffin for Processing and embedding
Hi All, I am thinking of switching the paraffin we are using for our processor and for embedding. We are using the Fisherbrand Paraplast Plus which contains DMSO for both the processor and for embedding. I used to use Suripath's paraplast and loved it. I would like to know what everyone in histoland is using. Thanks, Akemi Akemi Allison-Tacha BS, HT(ASCP)HTL Histology Manager APMG Laboratories 105A Cooper Court, Los Gatos, CA 95032 Contact: 800.848.2764 V/M: 408.884.2718 Fax: 408.884.2758 E-Mail: aallison-ta...@apmglab.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] (EDTA)Decal Bone marrows
Hi Patti, Years ago, I used a EDTA Decal Solution which was recommended by Dr. Jaffee, a Hematopathologist at NIH. This formula was particularly great for BM Core BX's. It is much gentler on the tissue, and is less likely to harm the cellular integrity. Dr. Todd Barry also prefers the use of EDTA formula's for BM Bx's. Below is the formula. or you can purchase from Biocare Medical. You should decal the specimen for 30 minutes to 1 hour and check for decalcification. DO NOT leave for any longer than 1 hour. 10% EDTA (Ethyl-media minetetra-acetic-acid Stock EDTA 10 ml DI water 90 ml pH 4.5-5.0 with Sodium Hydroxide If you prefer to purchase the IED Solution, Biocare Medical has it available. They have 2 sizes 140 ml or 500 ml. cat # IED 1204. Ion-Exchange Decalcification Unit (IED Unit, Designed for Bone Marrow Biopsies) An advanced decalcification system that removes calcium from bone quickly while leaving superior cellular detail. The IED Unit incorporates a strong cation ion-exchange resin in a weak acid solution to remove calcium ions from bone, replacing them with hydrogen ions. Because the IED Unit does not require strong concentrated acid solutions, as in traditional decalcification methods, delicate cellular structures antigenicity remain intact. Regards, Akemi Akemi Allison-Tacha BS, HT(ASCP)HTL Histology Manager APMG Laboratories 105A Cooper Court, Los Gatos, CA 95032 Contact: 800.848.2764 V/M: 408.884.2718 Fax: 408.884.2758 Cell: 408.335.9994 E-Mail: aallison-ta...@apmglab.com --- On Fri, 7/10/09, Patti Loykasek ployka...@phenopath.com wrote: From: Patti Loykasek ployka...@phenopath.com Subject: [Histonet] Bone marrows To: histonet histo...@pathology.swmed.edu Date: Friday, July 10, 2009, 12:38 PM Hi All. I was wondering what everyone's current favorite bone marrow decal solution/method is. Including commercially available decal solutions. We will be doing IHC after fixation/decal. Thanks for the input (as always). Patti Loykasek BS, HTL, QIHC Clinical Lab Supervisor PhenoPath Laboratories Seattle, WA This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Rotary Microtome's and the Meditome
Hi everyone in histoland, I am in the market for 3 microtome's. I was originally thinking of purchasing demo or refurbished Microm 355S units or comparable. I used this unit when I set-up a TMA lab and was very happy with it. I am in the process of demo'ing a Leica RM2255 unit. The Leica unit has all the bells and whistles the Microm microtomes has, but it is on a separate device vs Microm's features are created internally. I would like your thoughts on this. Also, I was given some information on a NEW Microtome called the Meditome Rotary Microtome. It is made in Germany by Medite and distributed by Micron, based in San Diego. It is my understanding that the engineers who made the Reichert Jung and the Microm microtomes developed this new microtome. It sells for under $10,000 and has a 2 year warranty vs the normal 1 year warranty. Although it doesn't have all the fancy features, it looks like a solid unit. Has anyone purchased one of these units, and if so, how do you like it? Thanks, Akemi Akemi Allison-Tacha BS, HT(ASCP)HTL Histology Manager APMG Laboratories 105A Cooper Court, Los Gatos, CA 95032 Contact: 800.848.2764 V/M: 408.884.2718 Fax: 408.884.2758 E-Mail: aallison-ta...@apmglab.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Resubmitting the question regarding 2011 CA Certification
Good Morning, I have not received any replies to my proposed question regarding the following question. I don't know if the histotech's that have the answers were on vacation, but I sure would like any information. Below is the original e-mail: I need your assistance on a on-going question which has been kicked around since 2005. I tried to look this up in the Histonet Archives, but was unsuccessful. Perhaps, my key words were incorrect. Anyway, it was my understanding that Governor Arnold Schwarzenegger put a mandate on all laboratory staff, including histologists, that they were required to be certified by 2011. It was further my understanding that un-certified histology staff were only going to be able to work as histology assistants. Could I get some clarification on this matter. Thanks, Akemi Allison-Tacha BS, HT/HTL ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] 2011 mandate for CA histology certification
Good Morning Histoland and Happy Friday! I need your assistance on a on-going question which has been kicked around since 2005. I tried to look this up in the Histonet Archives, but was unsuccessful. Perhaps, my key words were incorrect. Anyway, it was my understanding that Governor Arnold Schwarzenegger put a mandate on all laboratory staff, including histologists, that they were required to be certified by 2011. It was further my understanding that un-certified histology staff were only going to be able to work as histology assistants. Could I get some clarification on this matter. Thanks, Akemi Allison-Tacha BS, HT/HTL ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Fw: RE: I'm confused [Histonet] 2011 mandate for CA histology certification
Hi Donna, I'm confused! Are you addressing me because this was placed on the histonet? Regards, Akemi Akemi Allison-Tacha, BS, HT/HTL --- On Fri, 6/26/09, Akemi Allison-Tacha akemiat3...@yahoo.com wrote: From: Akemi Allison-Tacha akemiat3...@yahoo.com Subject: RE: [Histonet] 2011 mandate for CA histology certification To: Donna Willis do...@milestonemed.com Date: Friday, June 26, 2009, 2:23 PM Hi Donna, I'm confused! Are you addressing me? This was placed on the histonet. Regards, Akemi Allison-Tacha --- On Fri, 6/26/09, Donna Willis do...@milestonemed.com wrote: From: Donna Willis do...@milestonemed.com Subject: RE: [Histonet] 2011 mandate for CA histology certification To: Akemi Allison-Tacha akemiat3...@yahoo.com Date: Friday, June 26, 2009, 12:27 PM Would you please post your replies to the Histonet. Thanks, Donna Willis, HT/HTL(ASCP) North American Application Manager Milestone Medical (866) 995-5300 toll free (269) 488-4040 fax www.milestonemed.com Helping Patients -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Akemi Allison-Tacha Sent: Friday, June 26, 2009 9:29 AM To: histonet Subject: [Histonet] 2011 mandate for CA histology certification Good Morning Histoland and Happy Friday! I need your assistance on a on-going question which has been kicked around since 2005. I tried to look this up in the Histonet Archives, but was unsuccessful. Perhaps, my key words were incorrect. Anyway, it was my understanding that Governor Arnold Schwarzenegger put a mandate on all laboratory staff, including histologists, that they were required to be certified by 2011. It was further my understanding that un-certified histology staff were only going to be able to work as histology assistants. Could I get some clarification on this matter. Thanks, Akemi Allison-Tacha BS, HT/HTL ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Cell phone numbers go public next month
Hi All, Happy Friday! This is a little off the histology subject, but for those of you who have cell phones, might find this beneficial. Akemi Allison-Tacha BS, HT (ASCP) HTL Histology Manager Associated Pathology Medical Group Laboratories 105A Cooper Ct. Los Gatos, CA 95032Direct: 408.884.2718 Cell: 408.335.9994 W: E-Mail: aallison-ta...@apmglab.com P: E-Mail: akemiat3...@yahoo.com FYI Folks! REMEMBER: Cell Phone Numbers Go Public next month. All cell phone numbers are being released to telemarketing companies and you will start to receive sales calls. YOU WILL BE CHARGED FOR THESE CALLS Even if the message is saved on your phone, you will be charged for the minutes to listen to it. To prevent this, call the following number from your cell phone: 888-382-1222 It is the National DO NOT CALL list. It will only take a minute of your time. It blocks your number for five (5) years. You must call from the cell phone number you want to have blocked. You cannot call from a different phone number. HELP OTHERS BY PASSING THIS ON TO ALL YOUR FRIENDS. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] form for internal and external errors and corrective actions.
Hi All, I am updating several of our lab forms, and we do not have a form for internal and external errors and corrective actions. As you well know, this is a CAP requirement. Could any of you share your forms with me? Thanks a bunch, Akemi Akemi Allison-Tacha BS, HT (ASCP) HTL Histology Manager Associated Pathology Medical Group Laboratories 105A Cooper Ct. Los Gatos, CA 95032Direct: 408.884.2718 Cell: 408.335.9994 W: E-Mail: aallison-ta...@apmglab.com P: E-Mail: akemiat3...@yahoo.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Fw: Director, Laboratory Operations
Hi All, I was sent this opportunity for a Lab Operations position, but I already have a new job. I thought I would pass it on to anyone interested. Regards,Akemi Akemi Allison-Tacha BS, HT (ASCP) HTL Histology Manager Associated Pathology Medical Group Laboratories 105A Cooper Ct. Los Gatos, CA 95032Direct: 408.884.2718 Cell: 408.335.9994 W: E-Mail: aallison-ta...@apmglab.com P: E-Mail: akemiat3...@yahoo.com --- On Tue, 6/2/09, Steve Merwin s...@srasf.com wrote: From: Steve Merwin s...@srasf.com Subject: Director, Laboratory Operations To: akemiat3...@yahoo.com Date: Tuesday, June 2, 2009, 1:45 PM Dear Akemi, We have been retained by a dynamic and cutting edge molecular diagnostics based division of a Life Sciences company that provides competitive and personalized diagnosis toward cancer treatment. The company, which is well funded and financially stable, has retained us to help identify leaders for a Director of Laboratory Operations position that will be based in Southern California. This important and visible position within the organization will have responsibility for overseeing multiple clinical lab departments including flow cytometry, molecular diagnostics and client services comprising a group of up to 50. We are looking for candidates that possess the following: · BA/BS in Chemistry, Biology, Microbiology, Medical Technology or related field required. Advanced scientific degree desirable. · 10+ years post grad/industry experience. · 7+ years supervisory experience with a group of 30+, preferable in a commercial clinical laboratory. Experience overseeing molecular diagnostic testing would be viewed as a plus. · Strong inter-departmental presence and strategic outlook. · Experience ensuring regulatory compliance with federal and state agencies. The company offers an attractive compensation package as well as other benefits. Should you know of anyone who may be interested in hearing more about this opportunity, please contact me by telephone (925-472-6576) or reply by email. Thank you, in advance, for your time and help. Best regards, Steve Steve Merwin, C.P.C. Senior Executive Search Consultant Sanford Rose Associates - San Francisco 1255 Treat Blvd., Suite 300 Walnut Creek, CA 94597 Phone: 925-472-6576 Fax: 925-472-6579 email: s...@srasf.com linkedin: http://www.linkedin.com/in/stevemerwin website: www.srasf.com Finding People Who Make a Difference™” To be removed from our mailing list you may send a letter to the address listed above or send an email to rem...@srasf.com with REMOVE and include your Original Email Address/Addresses in the subject heading. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Fw: Re: AW: [Histonet] AFB Recycling Formalin for Fresh Tissue
Hello, I responded to Gudrun personally, but I thought that the information I am supplying him below might be a good educational source for other histologists who are having similar scenario's for AFB contamination. I would also like to say that I have an extremely eager histology team that have expressed an interest in learning theory and practice. Most of my team are OJT. They have had several pathologists approach them with these concerns regarding AFB, and because of their knowledge, or lack of it, couldn't fix the problem. We are starting with the basics and I am giving in-services along the way. Hi Gudrun, They are shipping the recycled formalin directly to the outside hospital surgery to put their fresh tissues into. As far as the AFB, we have had intermitant (+) AFB on the edges of tissues, that has been extremely questionable. We currently are not cutting a negative (-) positive(+) control on the same water bath as the test sample. That will be remedied beginning Monday. The water here is also in question. I will be sending it out for microbacteria analysis. We are going through a thorough housekeeping and in-service process. We are starting with the tissue processors, which are currently only changed weekly (not rotated daily), embedding centers, which have to my knowledge, never been drained, and the hopper cleaned-out. The water baths are not scrubbed out with soap and HOT water daily and covered at night to prevent contamination. The water bath is not skimmed of excess tissue debris, each and every time another specimen is placed on it. The Carbol Fuchsin is not being filtered prior to use. The working solution is currently being poured back into the stock bottle. Need I go on Akemi Allison-Tacha BS, HT (ASCP) HTL Histology Manager Associated Pathology Medical Group Laboratories 105A Cooper Ct. Los Gatos, CA 95032 Cell: 425.941.4287 W: E-Mail: aallison-ta...@apmglab.com P: E-Mail: akemiat3...@yahoo.com --- On Thu, 5/28/09, Gudrun Lang gu.l...@gmx.at wrote: From: Gudrun Lang gu.l...@gmx.at Subject: AW: [Histonet] Recycling Formalin for Fresh Tissue To: akemiat3...@yahoo.com Cc: histonet@lists.utsouthwestern.edu Date: Thursday, May 28, 2009, 10:34 AM Just for interest. Your lab takes the recycled formalin to make 4% neutral buffered formalin (NBF)? Or do they ship the recycled formalin directly in bottles to the surgery? AFB = acid fast bacili? Do you have concerns, that they produce false-positive AFB stains? I cannot imagine, that a (hopefully) small number of dead bacili on the surface of tissue will pretend this. An other question: Isn't there a filter in the recycler to prevent such contamination? Gudrun -Ursprüngliche Nachricht- Von: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von akemiat3...@yahoo.com Gesendet: Donnerstag, 28. Mai 2009 18:14 An: Histonet Betreff: [Histonet] Recycling Formalin for Fresh Tissue Good Morning Histo Land, I am asking all you out there to give me your input on recycIing formalin. I realize this has been discussed in the not too distant past, but this may be a little different situation. I realize some labs are recycling formalin to put on their tissue processors, but this is a totally different scenario. I have been a manager at my lab for only a month. Much to my surprise, I just found out yesterday that our lab is recycling formalin and shipping it out to one of our clients to put their FRESH SPECIMENS into. I just had a lengthy conversation with Robert Lott the day before yesterday, regarding pre-analytical protocol's effecting the test results of AFB. By the way, we were in full agreement. This formalin issue was unknown to me then. I realize that the pre-analytical, pre-analytical, pre-analytical, process effects the final out come. I need to present my case to our Director of Pathology why this is compromising patient care. Any and all responses are gladly welcome. Thanks, Akemi Akemi Allison-Tacha BS, HT (ASCP) HTL Histology Manager Associated Pathology Medical Group Laboratories 105A Cooper Ct. Los Gatos, CA 95032 Cell: 425.941.4287 W: E-Mail: aallison-ta...@apmglab.com P: E-Mail: akemiat3...@yahoo.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: Fw: Re: AW: [Histonet] AFB Recycling Formalin for Fresh Tissue
Thanks Freida, What better source to validate the steps I am taking to eliminate the problem of AFB Contaminate. I would also like to mention in my haste to compose my e-mail, I may have stated something incorrectly. I did not mean that a positive (+) control be cut at the same time as the test sample and negative (-) control. I usually place the test tissue on the same pre-cut positive (+) control slide. Thanks Rene, For your input on recycling formalin. This is good ammunition for my defense in stopping the use of recycling formalin. I still would welcome any and all replies. Akemi Akemi Allison-Tacha BS, HT (ASCP) HTL Histology Manager Associated Pathology Medical Group Laboratories 105A Cooper Ct. Los Gatos, CA 95032 Cell: 425.941.4287 W: E-Mail: aallison-ta...@apmglab.com P: E-Mail: akemiat3...@yahoo.com --- On Thu, 5/28/09, Freida Carson frei...@sbcglobal.net wrote: From: Freida Carson frei...@sbcglobal.net Subject: Re: Fw: Re: AW: [Histonet] AFB Recycling Formalin for Fresh Tissue To: Histonet Histonet@lists.utsouthwestern.edu, Akemi Allison-Tacha akemiat3...@yahoo.com Date: Thursday, May 28, 2009, 12:09 PM Wang reported on the contamination of tissue sections with AFB by the use of fluorescence microscopy, and if I remember correctly, when he tested the water in water fountains, he found something like 33% contained AFB. Non-pathogenic, but we can't tell that on our stains. The paper is in Am J Clin Pathol 51:71, 1969. We (Carson, Kingsley, Haberman, and Race) also reported the contamination in the 1964 issue of the same journal. We stopped using tap water for our water baths and began using millipore filtered water that had already been through a deionizing column and charcoal filter. We always cut a negative control from the same days workload as the patient case - uterus in our case. You don't need a positive control cut the same day, just a positive control with only a medium number of organisms. We also did not use any tap water in the deparaffinization prior to the carbol fuchsin. AFB organisms have also been reported growing in 40 gal formalin tanks believe it or not and in the old Fisher Paraffin wafers. We centrifuged tap water in 50-ml tubes, poured off the supernatent, and refilled and recentrifuged until we could see some sediment in the bottom. We took that to Microbioloby and had it cultured to definited prove the presence of AFB in the tap water. I would recommend this approach to anyone suspecting tissue contamination. Freida Carson --- On Thu, 5/28/09, Akemi Allison-Tacha akemiat3...@yahoo.com wrote: From: Akemi Allison-Tacha akemiat3...@yahoo.com Subject: Fw: Re: AW: [Histonet] AFB Recycling Formalin for Fresh Tissue To: Histonet Histonet@lists.utsouthwestern.edu Date: Thursday, May 28, 2009, 1:21 PM Hello, I responded to Gudrun personally, but I thought that the information I am supplying him below might be a good educational source for other histologists who are having similar scenario's for AFB contamination. I would also like to say that I have an extremely eager histology team that have expressed an interest in learning theory and practice. Most of my team are OJT. They have had several pathologists approach them with these concerns regarding AFB, and because of their knowledge, or lack of it, couldn't fix the problem. We are starting with the basics and I am giving in-services along the way. Hi Gudrun, They are shipping the recycled formalin directly to the outside hospital surgery to put their fresh tissues into. As far as the AFB, we have had intermitant (+) AFB on the edges of tissues, that has been extremely questionable. We currently are not cutting a negative (-) positive(+) control on the same water bath as the test sample. That will be remedied beginning Monday. The water here is also in question. I will be sending it out for microbacteria analysis. We are going through a thorough housekeeping and in-service process. We are starting with the tissue processors, which are currently only changed weekly (not rotated daily), embedding centers, which have to my knowledge, never been drained, and the hopper cleaned-out. The water baths are not scrubbed out with soap and HOT water daily and covered at night to prevent contamination. The water bath is not skimmed of excess tissue debris, each and every time another specimen is placed on it. The Carbol Fuchsin is not being filtered prior to use. The working solution is currently being poured back into the stock bottle. Need I go on Akemi Allison-Tacha BS, HT (ASCP) HTL Histology Manager Associated Pathology Medical Group Laboratories 105A Cooper Ct. Los Gatos, CA 95032 Cell: 425.941.4287 W: E-Mail: aallison-ta...@apmglab.com P: E-Mail: akemiat3...@yahoo.com --- On Thu, 5/28/09, Gudrun Lang gu.l...@gmx.at wrote: From: Gudrun Lang gu.l...@gmx.at Subject: AW: [Histonet] Recycling Formalin for Fresh
[Histonet] (Tissue Processor's) Used, Refurbished or Lease Agreement
Good Morning out there in Histo Land! Calling all Vendors. I have recently accepted a new position as the Histology Manager at APMG Labs in Los Gatos, CA. We are down one Tissue Processor. The Executive Administrator thought we would be purchasing a used VIP from one of our affiliates, but unfortunately this possibility has fallen through. I would appreciate any Vendors who have Tissue Processor's for sale that are Used or Refurbished. I am also open to the possibility of negotiating lease agreements. I realize warranties and service agreements are extremely important. Please e-mail me any available options. Thanks,Akemi Akemi Allison-Tacha BS, HT (ASCP) HTL Histology Manager Associated Pathology Medical Group Laboratories 105A Cooper Ct. Los Gatos, CA 95032 W: (408)884.2718 W: E-Mail: aallison-ta...@apmglab.com P: E-Mail: akemiat3...@yahoo.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Microtome Alignment
David Davis from CO designed an alignment device and can be purchased from Newcomer supply or I think his e-mail is still the following: davidda...@uchsc.edu Good Luck, Akemi Allison-Tacha BS, HT (ASCP) HTL Histology Manager Associated Pathology Medical Group Laboratories 105A Cooper Ct. Los Gatos, CA 95032 Cell: 425.941.4287 W: E-Mail: aallison-ta...@apmglab.com P: E-Mail: akemiat3...@yahoo.com --- On Thu, 5/7/09, kristen arvidson arvidsonkris...@yahoo.com wrote: From: kristen arvidson arvidsonkris...@yahoo.com Subject: [Histonet] Microtome Alignment To: histonet histonet@lists.utsouthwestern.edu Date: Thursday, May 7, 2009, 6:24 AM Does anyone know where I can an alignment device? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Job Descriptions and Annual Performance Appraisal Forms
Sorry, I forgot to put the Subject Hi All Histology Manager's, I would like to ask you for your assistance. I started my new position at APMG Labs yesterday, and one of the 1st requests given to me was by the HR Manager. She is in the process of updating and revising the Job Descriptions and the Annual Performance Appraisal Forms for all the Histology Staff. The positions are for the following: Histology Manager Histology Coordinator Lead Histologist Histotechnician (Non-Registered) Histology Lab Assistant Histology QC/Lab Assistant Grossing Assistant For the Annual Performance Appraisal Forms, she would like to separate each positions tasks for evaluation. If any of you would like to share your existing forms, I would greatly appreciate any and all assistance. Thank You in advance for your assistance, Akemi Akemi Allison-Tacha BS, HT (ASCP) HTL Histology Manager Associated Pathology Medical Group Laboratories 105A Cooper Ct. Los Gatos, CA 95032 Cell: 425.941.4287 E-Mail: aallison-ta...@apmg.com Akemi Allison-Tacha BS, HT (ASCP) HTL Histology Manager Associated Pathology Medical Group Laboratories 105A Cooper Ct. Los Gatos, CA 95032 Cell: 425.941.4287 E-Mail: aallison-ta...@apmg.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Wrong work e-mail address for Job Discriptions
Hi All, Sorry, My Bad! For those of you who were kind enough to supply me with information regarding Job Descriptions and Performance Appraisal Forms to my work e-mail address. Guess I made a mistake on my new work e-mail address. Below is my correct e-mail address. I would appreciate any information resent to either my personal or work e-mail. Thanks a bunch! Akemi Akemi Allison-Tacha BS, HT (ASCP) HTL Histology Manager Associated Pathology Medical Group Laboratories 105A Cooper Ct. Los Gatos, CA 95032 Cell: 425.941.4287 W: E-Mail: aallison-ta...@apmglab.com P: E-Mail: akemiat3...@yahoo.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet]( FE on BM Cores)Effects of decalcification for special stains
Don't forget the effects on decalcified Bone Marrow Cores. Even if you use EDTA decalcification solution, there will be diminished FE staining on Bone Marrow Cores or any other tissue that is decalcified and then stained for FE. Regards,Akemi Akemi Allison-Tacha BS, HT(ASCP)HTL Histology Manager Associated Pathology Medical Group Laboratories 105A Cooper Ct. Los Gatos, CA 95032 Cell: 425.941.4287 E-Mail: akemiat3...@yahoo.com --- On Thu, 4/9/09, Rene J Buesa rjbu...@yahoo.com wrote: From: Rene J Buesa rjbu...@yahoo.com Subject: Re: [Histonet] Effects of decalcification for special stains To: histonet@lists.utsouthwestern.edu, kyle ayres ayresk...@yahoo.com Date: Thursday, April 9, 2009, 1:53 PM Usually not, unless the tissues remain too much time in the decalcifyier, meaning more time than needed to decalcify. For IHC the adverse effect is greater and because of that, when possible, the decalficication should be done with EDTA. René J. --- On Thu, 4/9/09, kyle ayres ayresk...@yahoo.com wrote: From: kyle ayres ayresk...@yahoo.com Subject: [Histonet] Effects of decalcification for special stains To: histonet@lists.utsouthwestern.edu Date: Thursday, April 9, 2009, 3:31 PM Are there negative effects associated with acid decalcification and special stains, for example, AFB, GMS, and PAS (for fungus). All tissues are FFPE. Thanks, Kyle Ayres HT BS Nacogdoches Memorial Hospital ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: BEWARE aa aa staying with a link
Hi,Late last night I saw the posting from aa aa regarding staying, and there was a link, which I stupidly linked on to. What a BIG MISTAKE! It is a musical video. I started listening to it and it was cute, then I tried to close it. It stayed on, and on, and on! I have a Mac Book Pro Laptop, and I couldn't even do a force quit! I had to remove the battery and do a start-up cycle. I don't know if anyone else has had this problem, but DON'T TAKE THE CHANCE! Akemi Akemi Allison-Tacha BS, HT (ASCP) HTL Histology Manager Associated Pathology Medical Group Laboratories 105A Cooper Court, Los Gatos, CA 95032 Cell: (425) 941-4287 E-Mail: akemiat3...@yahoo.com --- On Wed, 4/8/09, Andrea Grantham algra...@email.arizona.edu wrote: From: Andrea Grantham algra...@email.arizona.edu Subject: Re: [Histonet] histonet off-topic comments To: LINDA MARGRAF linda.marg...@childrens.com Cc: histonet@lists.utsouthwestern.edu, MKing mak...@ufl.edu Date: Wednesday, April 8, 2009, 7:18 AM Linda, This really is too much - now I have this thing on my computer maliciously put there by aa aa that I can not get to close. More than one person has to be removed from the list and if Bernie was removed why are there emails from him this morning? Histonet has helped me so much but I'm afraid this is the last straw. I'm going to have to unsubscribe. Sad to see such a wonderful resource go down the tubes because of a handful of inconsiderate bastards. Andi Grantham Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cell Biology and Anatomy Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algra...@email.arizona.edu Tel: 520.626.4415 Fax: 520.626.2097 happy slicing and dicing and may all your stains work perfectly - Paula Sicurello On Apr 7, 2009, at 9:52 AM, LINDA MARGRAF wrote: Dear Histonet members: I have removed Bernie Taupin from the list. Let's please get back to the topic of histology and related fields.Thanks Linda M Histonet administrator MKing mak...@ufl.edu 4/7/2009 8:43 AM a motion is hereby offered to have the active members of histonet vote this troll off the island, or at least request that the list moderator do so. this person has proven to have absolutely nothing of value to contribute to this group, and is only intent on disrupting it. second? - Message: 16 Date: Mon, 6 Apr 2009 15:43:07 -0700 (PDT) From: Bernie Taupin bernietau...@ymail.com Subject: Re: [Histonet] Nice one Kemlo To: histonet@lists.utsouthwestern.edu Message-ID: 453473.65848...@web43516.mail.sp1.yahoo.com Content-Type: text/plain; charset=iso-8859-1 Instead of spitting your venom try to learn Sort of like you might consider learning correct English? I mean, its one thing if its not your first language, but despite how many people on here called you kindly and helpful, I find it perplexing that you feel compelled to call me a piece of ignorant, and TWICE, no less. I do not speak French. This list is not in French. Ergo, my awareness (or lack thereof) of proper French diction has absolutely nothing at all to do with anything... aside form providing you a vehicle to call me names and lash out. If you have something to say, say it. The simple fact that you do not like me does not give you- or anyone else- wholesale right to insult me or call me names. You should be ashamed of yourself, Rene. I never expected such antisocial behavior from you, and frankly, when you behave so regrettably, I feel no compulsion to learn anything form you! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet /pre span style=font-weight: bold;Please consider the environment before printing this e-mail/spanbr / br / span style=font-size: 8pt;This e-mail, facsimile, or letter and any files or attachments transmitted with it containsbr / information that is confidential and privileged. This information is intended only for the use of the br / individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further br / disclosures are prohibited without proper authorization. If you are not the intended recipient, any br / disclosure, copying, printing, or use of this information is strictly prohibited and possibly a br / violation of federal or state law and regulations. If you have received this information in error, br / please notify Children's Medical Center Dallas immediately at 214-456- or via e-mail at br / priv...@childrens.com. Children's Medical Center Dallas and its affiliates hereby claim all br / applicable privileges related to this information./spanbr / br / /html ___ Histonet mailing list Histonet@lists.utsouthwestern.edu
Re: RE: [Histonet] Virus planted ? BEWARE aa aa staying with a link
Hi All, I guess I was stupid to link on this file. I just hope aa aa didn't do this as an act of malicious behavior, and now we have viruses in our computers! I usually delete any unknown sender, and fortunately my MAC filters out most spams. Now I will have to do a diagnostic check. I recommend any of you who foolishly opened the link to do the same. Regards,Akemi Akemi Allison-Tacha BS, HT (ASCP) HTL Histology Manager Associated Pathology Medical Group Laboratories 105A Cooper Court, Los Gatos, CA 95032 Cell: (425) 941-4287 E-Mail: akemiat3...@yahoo.com --- On Wed, 4/8/09, Jane M Flinn jfl...@gmu.edu wrote: From: Jane M Flinn jfl...@gmu.edu Subject: Re: RE: [Histonet] Re: BEWARE aa aa staying with a link To: Martin, Gary gmar...@marshallmedical.org Cc: Akemi Allison-Tacha akemiat3...@yahoo.com, LINDA MARGRAF linda.marg...@childrens.com, Andrea Grantham algra...@email.arizona.edu, histonet@lists.utsouthwestern.edu, MKing mak...@ufl.edu Date: Wednesday, April 8, 2009, 10:21 AM I had to turn off my PC. Luckily that killed it. jane Life is short - make haste to be kind Dr. Jane Flinn Director, Undergraduate Neuroscience Program George Mason University, 3F5 4400 University Dr. Fairfax, VA 22030 Phone: 703-993-4107 Fax: 703-993-1359 - Original Message - From: Martin, Gary gmar...@marshallmedical.org Date: Wednesday, April 8, 2009 11:38 am Subject: RE: [Histonet] Re: BEWARE aa aa staying with a link If you have clicked on the aa aa attachment and can't get out of the loop ... use the ALT control delete buttons pushed simultaneously and you will get to the task manager ... highlight the attachment and click the button that says end task now that should get you out of the loop. Gary (California) -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet- boun...@lists.utsouthwestern.edu] On Behalf Of Akemi Allison-Tacha Sent: Wednesday, April 08, 2009 8:17 AM To: LINDA MARGRAF; Andrea Grantham Cc: histonet@lists.utsouthwestern.edu; MKing Subject: [Histonet] Re: BEWARE aa aa staying with a link Hi,Late last night I saw the posting from��aa aa�regarding staying, and there was a link, which I stupidly linked on to. �What a BIG MISTAKE! �It is a musical video. �I started listening to it and it was cute, then I tried to close it. It stayed on, and on, and on! �I have a Mac Book Pro Laptop, and I couldn't even do a force quit! �I had to remove the battery and do a start-up cycle. �I don't know if anyone else has had this problem, but DON'T TAKE THE CHANCE! Akemi Akemi Allison-Tacha BS, HT (ASCP) HTL Histology Manager Associated Pathology Medical Group Laboratories 105A Cooper Court, Los Gatos, CA 95032 Cell: (425) 941-4287 E-Mail: akemiat3...@yahoo.com --- On Wed, 4/8/09, Andrea Grantham algra...@email.arizona.edu wrote: From: Andrea Grantham algra...@email.arizona.edu Subject: Re: [Histonet] histonet off-topic comments To: LINDA MARGRAF linda.marg...@childrens.com Cc: histonet@lists.utsouthwestern.edu, MKing mak...@ufl.edu Date: Wednesday, April 8, 2009, 7:18 AM Linda, This really is too much - now I have this thing on my computer maliciously put there by aa aa that I can not get to close. More than one person has to be removed from the list and if Bernie was removed why are there emails from him this morning? Histonet has helped me so much but I'm afraid this is the last straw. I'm going to have to unsubscribe. Sad to see such a wonderful resource go down the tubes because of a handful of inconsiderate bastards. Andi Grantham Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cell Biology and Anatomy Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algra...@email.arizona.edu Tel: 520.626.4415� ���Fax: 520.626.2097 happy slicing and dicing and may all your stains work perfectly - Paula Sicurello On Apr 7, 2009, at 9:52 AM, LINDA MARGRAF wrote: Dear Histonet members: I have removed Bernie Taupin from the list. Let's please get back to the topic of histology and related fields.Thanks Linda M Histonet administrator MKing mak...@ufl.edu 4/7/2009 8:43 AM a motion is hereby offered to have the active members of histonet vote this troll off the island, or at least request that the list moderator do so.� this person has proven to have absolutely nothing of value to contribute to this group, and is only intent on disrupting it. second? - Message: 16 Date: Mon, 6 Apr 2009 15:43:07 -0700 (PDT) From: Bernie Taupin bernietau...@ymail.com Subject: Re: [Histonet] Nice one Kemlo To: histonet@lists.utsouthwestern.edu Message-ID: 453473.65848...@web43516.mail.sp1.yahoo.com Content-Type: text/plain; charset=iso-8859-1 Instead of spitting your venom try to learn Sort of like you might consider learning correct English? I mean, its one
Re: [Histonet] FREEZY spray
Bernie, I used to use the method below for FS on muscles at Emanual Hospital in Portland OR. Since we had such wonderful success, we incorporated this method for all FS. Years later, I developed the MATSSE (pH3.4) 1-Step Trichrome Stain Kit (Modified Trichrome Method for Muscle Biopsies Cut on Frozen Sections) for Biocare Medical. This kit has long since discontinued by the way...This came from Biocare's Data Sheet. NOTE: Muscle tissue should be suitably obtained and frozen within 30 minutes after excision. The usual method of freezing is to first mount a transverse section of the muscle on a chuck using 10% tragacanth gum or equivalent commercial frozen section mounting media as the adhesive. The chuck with the mounted specimen is then immersed in prechilled isopentane until frozen. Isopentane is precooled when a container of the substance is placed into liquid nitrogen. At a temperature of -160° C, the isopentane has a slightly syrupy consistency. Care should be taken to remove the muscle sample when freezing is complete, usually after 25 to 30 seconds. Too short a freezing time produces artifacts; prolonged freezing can produce cracking of the block. NOTE: If the sample cannot be sectioned immediately after freezing, it may be wrapped in foil and placed in a plastic-lidded container along with a small amount of ice for moisture. Specimens may be stored at -70° C until sectioned. Regards,Akemi Akemi Allison-Tacha BS, HT (ASCP) HTL Histology Manager Associated Pathology Medical Group Laboratories 105A Cooper Court, Los Gatos, CA 95032 Cell: (425) 941-4287 E-Mail: akemiat3...@yahoo.com --- On Tue, 4/7/09, Bernie Taupin bernietau...@ymail.com wrote: From: Bernie Taupin bernietau...@ymail.com Subject: Re: [Histonet] FREEZY spray To: Jennifer MacDonald jmacdon...@mtsac.edu, Ingles Claire cing...@uwhealth.org Cc: Histonet@lists.utsouthwestern.edu, histonet-boun...@lists.utsouthwestern.edu Date: Tuesday, April 7, 2009, 9:06 PM Does this apply to Liquid Nitrogen as well? I'd hate to have to try to cut fatty sections in the cryostat without it! I do mostly cryo, and I've never used liquid nitrogen to chill anything within the cryostat. What's that all about? I find that it leaves to many ice crystal artifacts anyway. Not cold enough fast enough. You might be making it harder on yourself... just set the temperature of the cryostat for the appropriate temperature of whatever tissue youre cutting, and you should have no need for a crutch, whether it be fluoroethane or liquid nitrogen. kisses, flowers and rainbows, Bernie Taupin, King of Cryomicrotomy, Esq. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] FREEZY spray
Sorry, I too noticed that the text looked weird then I did a cut and paste. I keep all the Data Sheets I created in my files, and this was just a portion of the Notes from the original Data sheet. I thought it might be helpful because you stated you had difficulty with cracking. You did not mention using isopentane. Some people immerse the tissue straight into liquid nitrogen, which could cause freezing artifacts. Akemi Allison-Tacha BS, HT (ASCP) HTL Histology Manager Associated Pathology Medical Group Laboratories 105A Cooper Court, Los Gatos, CA 95032 Cell: (425) 941-4287 E-Mail: akemiat3...@yahoo.com --- On Tue, 4/7/09, Bernie Taupin bernietau...@ymail.com wrote: From: Bernie Taupin bernietau...@ymail.com Subject: Re: [Histonet] FREEZY spray To: Akemi Allison-Tacha akemiat3...@yahoo.com, Jennifer MacDonald jmacdon...@mtsac.edu, Ingles Claire cing...@uwhealth.org Cc: Histonet@lists.utsouthwestern.edu, histonet-boun...@lists.utsouthwestern.edu Date: Tuesday, April 7, 2009, 10:11 PM I use isopentane suspended in liquid nitrogen, too. sorry if this sounds curt, but due to your cut-and-pasting, im not entirely sure what point youre trying to get at...? From: Akemi Allison-Tacha akemiat3...@yahoo.com To: Jennifer MacDonald jmacdon...@mtsac.edu; Ingles Claire cing...@uwhealth.org; Bernie Taupin bernietau...@ymail.com Cc: Histonet@lists.utsouthwestern.edu; histonet-boun...@lists.utsouthwestern.edu Sent: Wednesday, April 8, 2009 1:03:04 AM Subject: Re: [Histonet] FREEZY spray Bernie, I used to use the method below for FS on muscles at Emanual Hospital in Portland OR. Since we had such wonderful success, we incorporated this method for all FS. Years later, I developed the MATSSE (pH3.4) 1-Step Trichrome Stain Kit (Modified Trichrome Method for Muscle Biopsies Cut on Frozen Sections) for Biocare Medical. This kit has long since discontinued by the way...This came from Biocare's Data Sheet. NOTE: Muscle tissue should be suitably obtained and frozen within 30 minutes after excision. The usual method of freezing is to first mount a transverse section of the muscle on a chuck using 10% tragacanth gum or equivalent commercial frozen section mounting media as the adhesive. The chuck with the mounted specimen is then immersed in prechilled isopentane until frozen. Isopentane is precooled when a container of the substance is placed into liquid nitrogen. At a temperature of -160° C, the isopentane has a slightly syrupy consistency. Care should be taken to remove the muscle sample when freezing is complete, usually after 25 to 30 seconds. Too short a freezing time produces artifacts; prolonged freezing can produce cracking of the block. NOTE: If the sample cannot be sectioned immediately after freezing, it may be wrapped in foil and placed in a plastic-lidded container along with a small amount of ice for moisture. Specimens may be stored at -70° C until sectioned.. Regards,Akemi Akemi Allison-Tacha BS, HT (ASCP) HTL Histology Manager Associated Pathology Medical Group Laboratories 105A Cooper Court, Los Gatos, CA 95032 Cell: (425) 941-4287 E-Mail: akemiat3...@yahoo..com --- On Tue, 4/7/09, Bernie Taupin bernietau...@ymail.com wrote: From: Bernie Taupin bernietau...@ymail.com Subject: Re: [Histonet] FREEZY spray To: Jennifer MacDonald jmacdon...@mtsac.edu, Ingles Claire cing...@uwhealth.org Cc: Histonet@lists.utsouthwestern.edu, histonet-boun...@lists.utsouthwestern.edu Date: Tuesday, April 7, 2009, 9:06 PM Does this apply to Liquid Nitrogen as well? I'd hate to have to try to cut fatty sections in the cryostat without it! I do mostly cryo, and I've never used liquid nitrogen to chill anything within the cryostat. What's that all about? I find that it leaves to many ice crystal artifacts anyway. Not cold enough fast enough. You might be making it harder on yourself... just set the temperature of the cryostat for the appropriate temperature of whatever tissue youre cutting, and you should have no need for a crutch, whether it be fluoroethane or liquid nitrogen. kisses, flowers and rainbows, Bernie Taupin, King of Cryomicrotomy, Esq. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Sara Sorry to see you go
Hi Sara, I for one will be sad to see your absence. Perhaps in the future, you will reconsider and join our group again. It is extremely unfortunate to see these grown men acting like FOOLS! I have been pretty much a voyeur as of late, unless I have something credible to say or ask, but I feel the urge to state my feelings as a fellow histologist. And by the way, there are a number of extremely prominent histologists and pathologists that have stated they are voyeurs too! They may not go on line, but they see your postings! They have all taken note of this stupid on-line communication. I don't think these individuals realize that the pathology community observes these communications, and this may come back to bite them in the butt later on down the line! It lowers the image of these individuals as professionals, and their names and verbiage are forever archived in our memories. Once you ring the bell, you can't un-ring it! The Histonet Forum was initially created and funded by histology vendors and concerned histologists to help us as a group. I maybe wrong, but I thought it's purpose was to expand our knowledge in this valuable profession. I have been utilizing this wonderful tool, since almost the beginning. It was an eye opening experience when I started seeing pathologists joining in. It made me realize that they too saw the merit in this wonderful tool for sharing our knowledge and experiences. I have been involved in histology since taking classes in histomicrotechnic at Tokyo University in 1968. The older I get, the more I realize how little I know. Especially with the use of molecular biology, and more and more esoteric testing. We are experiencing a metamorphosis in our field, and forums such as this, are extremely valuable! Those of us who use this tool, do so, because we care about giving the best possible patient care. At least I use to think so This is a wonderful tool for histologists, researchers, and concerned pathologists, to come together for the sole purpose to learn and grow (then there's the Friday good hearted release of steam) The Histonet is especially important for those individuals who are geographically isolated, and do not have the means to attend continuing education classes. It is my belief that the Histonet was never intended to be used as a tool as these individuals have so irresponsibly done. It is childish and unprofessional. This sort of use just shows how unthinking and selfish these individuals are. I am ashamed that the pathology community sees this sort of communication between histologists. It demeans us as a whole. I don't think these men realize what fallout may occur. We may have this taken away from us because they have not used their good judgement, common sense, and restraint! I realize that in the past, I too may have spoken a little out of turn, but this thread has become an embarrassment. I have recently accepted a employment opportunity to go back to managing a histology lab, and will ask my fellow histologists for their assistance. I truly hope there will be a Histonet available when I need you! Let's hope we can learn from this experience and move on. Regards,Akemi Akemi Allison-Tacha BS, HT (ASCP) HTL President Phoenix Lab Consulting Specializing in Histology, SS, IHC, TMA Tele: (425) 941-4287 E-Mail: akemiat3...@yahoo.com --- On Mon, 4/6/09, Breeden, Sara sbree...@nmda.nmsu.edu wrote: From: Breeden, Sara sbree...@nmda.nmsu.edu Subject: [Histonet] I'm outta here To: histonet@lists.utsouthwestern.edu Date: Monday, April 6, 2009, 8:43 AM After almost 5 years of Histonet membership, I am taking a break and unsubscribing. Why? Because I've had enough of the extraneous nonsense. I'll unsubscribe as required but I'm telling you why you won't be coming into this computer for a while. This listserv has so much potential to do good but some are just like the gangsta wannabees that use their smarts for crap. If I need help, I'll hit the books. Ta-ta for now. Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Roche/Genetech Today Buy-out and Lay-offs
FYI... This is from Todays Fierce Pharma Report: Today's Top Stories 1. Roche to cut jobs in California cowboys deal By Tracy Staton Comment | Forward Deal dissection of the day, No. 1: Now that Genentech has finally conceded to Roche's buyout plans, the postgame analysis begins. As we all know after months of following the possibility of a deal, preserving innovation at Genentech is job one. Next on the priority list comes cost-cutting. But neither of those goals will kick in if shareholders don't tender, so we're hearing plenty about their opinions. Here's a sampling of the predictions, prognositications, and pushy advice: Roche plans to shed more than 1,500 jobs in New Jersey and relocate major operations to California as part of the Genentech takeover. Some 1,600 researchers and scientists will stay behind in Nutley, NJ. But the company is redoing its campus master plan to figure out what to do with all the vacant buildings that are left. And who's first in line for the job cuts? None other than sales and marketing, the Star-Ledger says. After years of partnership, Roche and Genentech are already close. Merging their drug portolios should be a snap, New York Times DealBook says. But their cultures are almost diametrically opposed: Roche is Swiss; trains-running-on-time, precision-watchmaking Swiss. Genentech, as one Silicon Valley venture capitalist put it, are a bunch of entrepreneurial California cowboys. Get the image? Then you see the problem. Roche execs may be guzzling celebratory champagne (somehow we can't imagine it, but it's possible). But along with its buyout of Genentech's promising pipeline come worries about payer reimbursements. President Obama's push for comparative-effectiveness research could leave some Genentech drugs struggling, BusinessWeek reports. Some previous head-to-head studies have shown the benefits of expensive cancer biologics are small; if that trend continues, the bottom line could suffer. Akemi Allison-Tacha BS, HT (ASCP) HTL President Phoenix Lab Consulting Specializing in Histology, SS, IHC, TMA Tele: (425) 941-4287 E-Mail: akemiat3...@yahoo.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Apogent, Fisher/Thermo
Hi, FYI Posting from a TBS recent article: Quote: Fisher Scientific Merges with Apogent and then is acquired by Thermo As you have probably heard, Fisher Scientific, one of TBS' premier dealers, first merged with Apogent - an international manufacturing company having several divisions that produce products that compete directly with some of TBS' industry leading reagents and consumables, and, has more recently been acquired by Thermo, the parent company of Shandon. Akemi Allison-Tacha BS, HT (ASCP) HTL President Phoenix Lab Consulting Specializing in Histology, SS, IHC, TMA Tele: (425) 941-4287 E-Mail: akemiat3...@yahoo.com --- On Fri, 3/13/09, Golden State Acrylic Designs gsacrylicdesi...@gmail.com wrote: From: Golden State Acrylic Designs gsacrylicdesi...@gmail.com Subject: Re: [Histonet] Buy-outs Who owns Who To: akemiat3...@yahoo.com Date: Friday, March 13, 2009, 2:03 PM Apogent Tech was bought out by Thermo who bought Fisher. On Fri, Mar 13, 2009 at 1:14 PM, Akemi Allison-Tacha akemiat3...@yahoo.comwrote: Happy Friday the 13th Histonet! Forewarning, this is a little long. There seems to be a lot of discussion as of late, to all the buy outs, and who owns who. I have been following this trend for several years with great interest. It is amazing how pricing, customer support and service, varies from one company to another, even though they are owned by the same parent company. I can certainly understand and relate to the frustration and anger of the end-user. There is so much disconnection within the companies! We need a score card to keep up with all the changes! I maybe wrong, but I don't think the sales reps even know the ownership tree. I put a tree at the end of this correspondence that I think is correct. I recently had a conservation with a HR rep from Thermo/Fisher, and she didn't even know who Peter Scheu was. Pretty telling of the knowledge out there. HR should at least know who is at the top of the chain! Quote: Peter Scheu has been Group President, Clinical Diagnostics of Apogent Technologies Inc. since September 2000. Mr. Scheu served as the President of Richard-Allan Scientific Company (“Richard-Allan”) from 1997 to 2000 and as Executive Vice President of Richard-Allan from 1995 to 1997. Let's dispell the confusion and please correct me if I am wrong. The ownership trees: Parent Company: Apogent Technologies Inc Thermo/Fisher Richard Allan Microm Lab Vision Neomarkers Antibodies Parent Company: Danaher Leica Microsystems Vision Biosystems (Bond) Novacastra NEW-Suripath NEW-McCormick Regards, Akemi Akemi Allison-Tacha BS, HT (ASCP) HTL President Phoenix Lab Consulting Specializing in Histology, SS, IHC, TMA Tele: (425) 941-4287 E-Mail: akemiat3...@yahoo.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Fw: RE: Back to the Lab in SF
Hi Doug and Histonet, Thanks for the clarification on the company tree. You are absolutely correct! Have a great weekend fellow histonet subscribers! As for me, I'm packing and heading back to the SF Bay Area. I have accepted a position as a Histology Manager for a private reference lab. My heart has always been in the lab and patient care. Wish me luck, Akemi Akemi Allison-Tacha BS, HT (ASCP) HTL President Phoenix Lab Consulting Specializing in Histology, SS, IHC, TMA Tele: (425) 941-4287 E-Mail: akemiat3...@yahoo.com --- On Fri, 3/13/09, Akemi Allison-Tacha akemiat3...@yahoo.com wrote: From: Akemi Allison-Tacha akemiat3...@yahoo.com Subject: RE: [Histonet] Buy-outs Who owns Who To: Doug Showers doug.show...@propath.com Date: Friday, March 13, 2009, 1:56 PM Hi Doug, Thanks for the clarification. You are absolutely correct! Have a great weekend fellow histonet subscribers! As for me, I'm packing and heading back to the SF Bay Area. I have accepted a position as a Histology Manager for a private reference lab. My heart has always been in the lab and patient care. Wish me luck, Akemi Akemi Allison-Tacha BS, HT (ASCP) HTL President Phoenix Lab Consulting Specializing in Histology, SS, IHC, TMA Tele: (425) 941-4287 E-Mail: akemiat3...@yahoo.com --- On Fri, 3/13/09, Doug Showers doug.show...@propath.com wrote: From: Doug Showers doug.show...@propath.com Subject: RE: [Histonet] Buy-outs Who owns Who To: akemiat3...@yahoo.com Date: Friday, March 13, 2009, 12:33 PM You certainly do need a program to keep track of who is who. Didn't Fisher Scientific purchase Apogent and the Thermo purchased Fisher the next year to form Thermo Fisher Scientific? If so then Thermo should be the parent company. Doug Showers, MS, HT Histology Manager ProPath 8267 Elmbrook Dr. Suite 100 Dallas, TX 75247 214-237-1680 214-422-3083 Mobile 214-237-1706 FAX To learn more about ProPath, please visit http://www.ProPathLab.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Akemi Allison-Tacha Sent: Friday, March 13, 2009 12:15 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Buy-outs Who owns Who Happy Friday the 13th Histonet! Forewarning, this is a little long. There seems to be a lot of discussion as of late, to all the buy outs, and who owns who. I have been following this trend for several years with great interest. It is amazing how pricing, customer support and service, varies from one company to another, even though they are owned by the same parent company. I can certainly understand and relate to the frustration and anger of the end-user. There is so much disconnection within the companies! We need a score card to keep up with all the changes! I maybe wrong, but I don't think the sales reps even know the ownership tree. I put a tree at the end of this correspondence that I think is correct. I recently had a conservation with a HR rep from Thermo/Fisher, and she didn't even know who Peter Scheu was. Pretty telling of the knowledge out there. HR should at least know who is at the top of the chain! Quote: Peter Scheu has been Group President, Clinical Diagnostics of Apogent Technologies Inc. since September 2000. Mr. Scheu served as the President of Richard-Allan Scientific Company (Richard-Allan) from 1997 to 2000 and as Executive Vice President of Richard-Allan from 1995 to 1997. Let's dispell the confusion and please correct me if I am wrong. The ownership trees: Parent Company: Apogent Technologies Inc Thermo/Fisher Richard Allan Microm Lab Vision Neomarkers Antibodies Parent Company: Danaher Leica Microsystems Vision Biosystems (Bond) Novacastra NEW-Suripath NEW-McCormick Regards, Akemi Akemi Allison-Tacha BS, HT (ASCP) HTL President Phoenix Lab Consulting Specializing in Histology, SS, IHC, TMA Tele: (425) 941-4287 E-Mail: akemiat3...@yahoo.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] back to the lab in SF
Hi All, I have been receiving numerous inquires as to why I have left PhenoPath. I appreciate your inquires and congratulations. I am going to give a blanket reply, since I have time constraints. PhenoPath is a great company and I have the highest regard for Dr's. Allen Gown, Steve Kussick, and the rest of the staff. Sometimes there is a bigger plan in the cards for us, and my talents are more suited for the laboratory and direct patient care. I was offered a wonderful job in Los Gatos, CA, which is 30 minutes from Santa Cruz, and an hour from Carmel and the wine country. I love the Bay Area. I lived there for 8 years while working for Biocare as the Director of Histology, and I have a lot of friends there, plus weather is wonderful, and it's like going home! Regards, Akemi Akemi Allison-Tacha BS, HT (ASCP) HTL President Phoenix Lab Consulting Specializing in Histology, SS, IHC, TMA Tele: (425) 941-4287 E-Mail: akemiat3...@yahoo.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Fw: RE: Holiday Greetings from Chuck Churukian
Hi Histonet Subscriber's, The following forwarded e-mail was from Chuck Churukian. I spoke with his wife Irene last evening and she wished to have this Holiday wish forwarded to the histonet world. Sincerely, Akemi Akemi Allison-Tacha BS, HT (ASCP) HTL President Phoenix Lab Consulting Specializing in Histology, SS, IHC, TMA Tele: (425) 941-4287 E-Mail: akemiat3...@yahoo.com Subject: RE: Holiday Greetings To: akemiat3...@yahoo.com Date: Friday, December 19, 2008, 5:37 PM Thank you for your Greeting that included every religion, every circumstance, situation, sex orientation, etc. etc. It was very humorous. Here's our greeting As simple as we are... Names We Hold Dear by Vicki J. Kuyper (paraphrased) Every name is a touchstone that leads to a place and time, Where God has used another’s heart to reach out and touch mine. (ours) It may have happened years ago or even yesterday, But every person on our list has changed our lives some way. Through simple conversation, a warm hug or a shared meal, Every person on our list has helped us grow or in many ways- heal, Or laugh or love or learn or smile…..the blessings never end As God allows our paths to cross as family and/or friends. So please know that this greeting is more than a Christmas wish. It’s a “thank you” to God for putting on our list--- Each and everyone whose name we have come to hold dear…. Those who have shown us Christmas love – years passed, even this year. Have a wonderful holiday. Get some rest and relaxation Our wish and prayer for you: A Happy Healthy Prosperous New Year Chuck and Irene ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Happy Holidays
Happy Friday before Christmeas...I couldn't help but share this with you all! Happy Solstice, Happy Hanukkah, Merry Christmas, Happy Kwanza Sorry for the delay…our Legal Department just approved the following Holiday Greeting: Please accept with no obligation, implied or implicit my best wishes for an environmentally conscious, socially responsible, low stress, non-addictive, gender neutral, celebration of the winter solstice holiday (tm), practiced within the most enjoyable traditions of the religious persuasion of your choice, or secular practices of your choice, with respect for the religious/secular persuasions and/or traditions of others, or their choice not to practice religious or secular traditions at all . . . and a fiscally successful, personally fulfilling, and medically uncomplicated recognition of the onset of the generally accepted calendar year 2009, but not without due respect for the calendars of choice of other cultures whose contributions to society have helped make America great, (not to imply that America is necessarily greater than any other country or is the only AMERICA in the western hemisphere), and without regard to the race, creed, color, age, physical ability, religious faith, choice of computer platform, or sexual orientation of the wishee. By accepting this greeting, you are accepting these terms: This greeting is subject to clarification or withdrawal. It is freely transferable with no alteration to the original greeting. It implies no promise by the wisher to actually implement any of the wishes for her/himself or others, and is void where prohibited by law, and is revocable at the sole discretion of the wisher. This wish is warranted to perform as expected within the usual application of good tidings for a period of one year, or until the issuance of a subsequent holiday greeting, whichever comes first, and warranty is limited to replacement of this wish or issuance of a new wish at the sole discretion of the wisher. Have a Great 2009: Akemi Allison-Tacha BS, HT (ASCP) HTL President Phoenix Lab Consulting Specializing in Histology, SS, IHC, TMA Tele: (425) 941-4287 E-Mail: akemiat3...@yahoo.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Re: Fungal stains political views
Hi All, I totally agree with Robert on his information regarding periodic acid and chromic acid. I have done extensive research on this subject when I developed my special stain kits for Biocare Medical. I did incorporate 10% chromic acid for the GMS stain, and 1% periodic acid for the PAS stain. As far as this forum for political views, in frustration, I deleted everything with these headings. There could of been some very interesting feed-back, but you have used this source totally inappropriately! I believe in this countries beliefs for freedom of speech, but use it wisely!! The histonet is NOT THE PLACE! Akemi Allison-Tacha, BS, HT(ASCP)HTL Client Services Manager PhenoPath laboratories 551 North 34th Street, Suite 100 Seattle, WA 98103-8675 Work: (206) 374-9000 ext 1053 E-Mail: [EMAIL PROTECTED] --- On Wed, 11/5/08, Robert Richmond [EMAIL PROTECTED] wrote: From: Robert Richmond [EMAIL PROTECTED] Subject: [Histonet] Re: Fungal stains To: histonet@lists.utsouthwestern.edu Date: Wednesday, November 5, 2008, 7:29 PM Colin Weaver asks about staining of various fungal species by the standard fungal stains. The standard fungal stains have two basic steps: 1. Periodic acid or chromic acid splits bonds between vicinal diol groups (two carbons with OHs on adjacent carbon atoms) and oxidizes the free ends to aldehyde groups (-CHO). 2. The aldehyde groups oxidize Schiff's reagent or methenamine silver to produce a visible deposit. Periodic acid is not a sufficiently strong oxidant to demonstrate some fungal cell walls. In human pathology, Histoplasma is most likely to stain weakly or not at all. You need chromic acid, most usually followed by methenamine silver. Unfortunately, many so-called GMS kits silently substitute periodic acid for the more hazardous chromic acid. Freida Carson reviewed this top in J Histotechnol several years ago - I can find the reference if anyone needs it (I think I've posted it on Histonet before, so it's probably in the archives.) So your first step is to see if you're actually using chromic acid. PLEASE get the politics off this list - the repetitious garbage is making the list hard to read, particularly in digest form. Linda Margraf, if it doesn't stop, I suggest moderating the list for a few days, though not permanently. Bob Richmond Samurai Pathologist Knoxville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
