Re: [Histonet] Testing for hires

[Anne Murvosh via Histonet]

Melt down old blocks, have them embed and cut them. Evaluate their time and 
technique. You could also do a working interview where a tech shows them around 
but has them cut and embed a few current tissues while watching. Thanks Anne

Anne Murvosh
Histology Technician

 Three Locations to Serve YOU!
   Spokane Valley   Northside   
   Coeur d'Alene
1807 N Hutchinson Rd 59 E Queen Ave, Ste 1021700 W. Riverstone Drive
Spokane, WA 99212 Spokane, WA 99207Coeur d'Alene, ID 
83814

509-456-7414  Office
509-624-0763  Fax
amurv...@advancederm.net
http://www.advancederm.net

-Original Message-
From: Stephanie L. Thompson via Histonet 
Sent: Wednesday, November 16, 2022 10:18 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Testing for hires



When hiring for a histotech, how do you test their skills?

Thank you,

Stephanie
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[Histonet] Resumes for laboratory

[Anne Murvosh via Histonet]

I wanted to update my resume just to have in case of emergency. I have not done 
one in many years, and I know some formatting has changed. Does anyone have a 
good template that relates to the laboratory? Thanks Anne


Anne Murvosh
Histology Technician

 Three Locations to Serve YOU!
   Spokane Valley   Northside   
   Coeur d'Alene
1807 N Hutchinson Rd 59 E Queen Ave, Ste 1021700 W. Riverstone Drive
Spokane, WA 99212 Spokane, WA 99207Coeur d'Alene, ID 
83814

509-456-7414  Office
509-624-0763  Fax
amurv...@advancederm.net<mailto:amurv...@advancederm.net>
http://www.advancederm.net<https://exchange/owa/redir.aspx?C=dj3RNrpbyU2PF7XzpTT48jX3fGkVVOl0hDXSRb4uoQA8X_Nm4MTWCA..=http%3a%2f%2fwww.advancederm.net%2f>


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[Histonet] MOHS scheduling

[Anne Murvosh via Histonet]

I was wondering, for those doing Mohs cases, how far apart you schedule your 
patients, and if you do them throughout the day or just in am or pm. Thanks Anne


Anne Murvosh
Histology Technician

 Three Locations to Serve YOU!
   Spokane Valley   Northside   
   Coeur d'Alene
1807 N Hutchinson Rd 59 E Queen Ave, Ste 1021700 W. Riverstone Drive
Spokane, WA 99212 Spokane, WA 99207Coeur d'Alene, ID 
83814

509-456-7414  Office
509-624-0763  Fax
amurv...@advancederm.net<mailto:amurv...@advancederm.net>
http://www.advancederm.net<https://exchange/owa/redir.aspx?C=dj3RNrpbyU2PF7XzpTT48jX3fGkVVOl0hDXSRb4uoQA8X_Nm4MTWCA..=http%3a%2f%2fwww.advancederm.net%2f>


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[Histonet] Small processors

[Anne Murvosh via Histonet]

We are looking forward to ordering a new or used processor. We have the Sakura 
VIP5 however, I was wondering if there were smaller processors out there. We 
only do a max of about 60 skin specimens and the VIP is a bit large for that 
quantity. I would say a max of 100 specimens for future growth. Any thoughts. 
Thanks Anne Murvosh HT
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Re: [Histonet] Cryostat anti-roll plate

[Anne Murvosh via Histonet]

We have 3 new leicas and we constantly have to adjust them. They creep forward 
so much that I just automatically know to roll it slightly back after every 4 
specimens or so. The new leica designs are just bad and customer service is 
even worse. We have to use the plate as we do Mohs specimens and the sections 
are just better with the glass plate. I want to look for a different machine 
next time. We did test the Avantik and it cut nice but the glass plate was on 
the front and got in the way. I wish they would talk to Mohs techs before they 
design these. Oh well. No tricks just constant adjusting. Thanks Anne

-Original Message-
From: Ken Marzinsky via Histonet 
Sent: Tuesday, May 24, 2022 1:16 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Cryostat anti-roll plate

Hi everyone. Does anyone regularly use the anti-roll plate on their cryostat? 
Does it work well on the Leicas and Microms, or do you have to figit with it a 
lot? Does anyone have any tricks they can share to get it to work all the time? 
 I found that a little spritz of freeze spray on the plate and the bottom of 
the roll glass helps, but it is still seems a lot easier for us to use a brush 
or a teasing needle.

Ken
Durham, NC
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[Histonet] Once-a-month job opportunity

[Anne Murvosh via Histonet]

We have a position available that is just once-a-month in Walla Walla, 
Washington. This is a good position for someone retired or free on a Friday who 
wants some extra cash and lives in Walla Walla or nearby Tri-cities Washington. 
The hours are 7:30 am to when Mohs is completed between 12-1:00 pm.Two years' 
experience preferable. Thanks Anne Murvosh H.
Please Contact:
Advanced Dermatology & Skin Surgery, in connection with United Derm Partners
Mohs TECHNICIAN-WALLA WALLA, Washington
3RD Friday of every month
Please contact: Anne Marie Poole 
apo...@advancederm.net<mailto:apo...@advancederm.net>





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[Histonet] Stain line order

[Anne Murvosh via Histonet]

When doing frozen section staining for Mohs we have DI water and don't need a 
clearing agent. I am helping set up someone who is using tap water and needs to 
clear it. They mentioned that they used to clear first, rinse, then use bluing. 
I thought it was the opposite after heme. Rinse, bluing, rinse, clearing. I 
don't remember which is first. Actually do you need a bluing if using tap water 
since that blues better then DI water? Thanks for your input, it's been 10 
years since I had to think about this. Anne


Anne Murvosh
Histology Technician



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[Histonet] Mohs Tech Wage?

[Anne Murvosh via Histonet]

We are trying to hire a part-time Mohs technician in Washington state that is a 
train on the job.  I feel like the wage is too low, what are beginning Mohs 
techs making if they are not a histology tech? Thanks Anne
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[Histonet] Formalin question

[Anne Murvosh via Histonet]

I was wondering if 10% formalin needed to be kept in a flammable cabinet. We 
never have before, but I wondered if we were doing it wrong or if regulations 
have changed. Thanks Anne Murvosh HT.



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[Histonet] Cryostats for Mohs specimens

[Anne Murvosh via Histonet]

We are shopping for a new cryostat; has anyone used the Tanner Scientific (r) 
TN50 cryostat? Can you let me know if you like it. We are unhappy with the new 
Leica's. We are also looking at the Avantik ones as well. Any input is helpful; 
this is for Mohs specimens so they need to cut well and be able to get a lot of 
tissue on each slide, sometimes the components of the machine get in the way of 
good tissue pick-up. Thanks Anne Murvosh HT
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[Histonet] Mart-1 rapid test for Mohs specimens

[Anne Murvosh via Histonet]

I'm working up a price comparison for NovoDiax's rapid 10 minute Mart- 1 stain 
for melanoma on Mohs specimens. The doctors would like me to price shop but I 
only know of 40 minute stains which is too long. Does anyone know of any other 
companies running a rapid 10-15 minute Mart- 1 stain? Thanks Anne Murvosh (HT)
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[Histonet] Frozen tissue

[Anne Murvosh via Histonet]

I'm testing a faster Mart 1 for melanoma on Mohs specimens. Its been a while 
since I've done any frozen tissue IHC stains and I can't remember the fixation 
to keep it viable for testing the next day, or same day but later on. Will it 
hold up if fixed in acetone and dried or do I need to refrigerate or freeze? 
Also how long do they last,  I can't remember the protocol. Thanks Anne HT
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[Histonet] Xylene recycle

[Anne Murvosh via Histonet]

I'm looking to switch from a disposal pick-up to recycling of Xylene. Can 
anyone tell me some companies that offer a recycling unit. The catch is we 
don't have very much so I need something for a small lab. Thanks Anne
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[Histonet] Processor

[Anne Murvosh via Histonet]

I have a back-up processor we keep filled for a back-up and if we have an extra 
run. However with lower volumes lately we aren't using it. I was just wondering 
about the paraffin in particularly. Does anyone know if you are not using it 
how long it can stay there (its heated) before changing out completely? Does it 
lose its stability? Thanks Anne Murvosh (HT)
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[Histonet] Formaldehyde test kit question

[Anne Murvosh via Histonet]

We switched formalin neutralizing solution and I bought the Quantofix 
formaldehyde test kit to make sure it's working, however it does not mention 
what color is acceptable for neutralization. The options are 0-10-20- up to 200 
MG/L. should it be "0" for acceptable dumping? Also the instructions say to add 
10 drops of a sodium hydroxide solution to the solution being tested, wouldn't 
that change the Ph and make the results not as accurate. This kit is expensive 
if regular pH strips are better, let me know that too. Thanks for your help. 
Anne

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[Histonet] Xylene and Formalin monitoring

[Anne Murvosh via Histonet]

Just need a refresher on formalin and xylene monitoring regulations. Do we 
still have to do a 15 minute and 8 hour testing on both xylene and formalin, or 
can we just do the 8 hour. I wasn't sure if the regulations have changed or 
not. Thanks Anne
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[Histonet] Yearly review

[Anne Murvosh via Histonet]

Hey everyone, does the yearly employee competency assessments need to be kept 
for just 2 years or for the entire time an employee is there. I have some old 
ones I want to toss, but I just wanted to be sure. Thanks Anne
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Re: [Histonet] Help to interview new employees for the first time

[Anne Murvosh via Histonet]

I'm not sure if this is an option, but we used to melt down old blocks and make 
the HT embed and cut them. This showed us how good and quick they were and if 
they actually new what they were doing. Anne

-Original Message-
From: Blanca Lopez via Histonet  
Sent: Thursday, September 12, 2019 11:48 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Help to interview new employees for the first time

I am going to interview people for Histotech position for the first time...what 
are the best questions to ask? How do I prepare myself? what is the I need to 
know that they are the best one? What should I ask or choose? Is good to put 
them in action like cutting or staining to check on their skills or what are 
your recommendations? thank you for your help

Blanca Lopez HT (ASCP)cm
Senior Histotechnologist
UT Southwestern Medical Center
Harold C. Simmons Comprehensive Cancer Center UTSTR Biorepository Tissue Lab
6000 Harry Hines Blvd NB5.102
Dallas, Texas 75390
214-648-7598
blanca.lo...@utsouthwestern.edu





UT Southwestern


Medical Center



The future of medicine, today.

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[Histonet] Grossing large ellipse melanomas

[Anne Murvosh via Histonet]

We are starting to keep our melanomas in-house now that we have a better 
processor, however I could use some tips or tricks on cutting large fatty skin 
ellipse specimens. My problem I think, is too small a knife, the specimens are 
not cutting even and the fat is getting in the way. So any suggestions or links 
to grossing techniques would be appreciated. Also, any ideas on better cutting 
utensils for these. Thanks Anne Murvosh HT
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[Histonet] Gout specimens

[Anne Murvosh via Histonet]

I haven't done a gout specimen in years, I remember the processing part, not 
remembering the Staining part. Could someone please send me your gout protocol? 
Thanks Anne Murvosh HT

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[Histonet] Processors

[Anne Murvosh via Histonet]

Can anyone recommend a processor for a smaller lab. I need one for about 150 to 
200 specimens. It can be a floor model or tabletop preferably a used model. 
Processing skin specimens only, I currently have a leica TP 1020. Venders 
welcome to reply. Thanks for your input, Anne
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[Histonet] Temp employees

[Anne Murvosh via Histonet]

Does anyone know of a company that provides temp employees for a Mohs 
technician? We need to cover someone for a little over a month in February or 
March of next year. Thanks Anne Murvosh
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[Histonet] CEU's

[Anne Murvosh via Histonet]

I was wondering if college classed not related to histology counted for 
continuing education. I'm going back to school and although I think science 
classes would count, I wasn't sure about  a math or English class. Does anyone 
know for sure. Thanks Anne
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[Histonet] Slide disposal

[Anne Murvosh via Histonet]

When disposing of slides after the 10 period is up, what is everyone doing to 
protect patient confidentiality? In the old days we would just put packing tape 
around the box and toss. With patient names on the slides and sometimes 
specimen location on the new labels I'm wondering how to dispose of them 
properly. Thanks Anne
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Re: [Histonet] Pathology Ladder System

[Anne Murvosh via Histonet]

If you don't already have it you may want to offer a shift differential for 
very early or late shifts and weekends.
This makes it a little easier to hire for and keep people on the harder shifts. 
 Anne

-Original Message-
From: Steve via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Tuesday, August 09, 2016 4:20 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Pathology Ladder System

Good morning everyone.  I had a meeting recently with hospital administration 
where I was asked what leadership could do to retain and keep quality staff.  
Besides the obvious, pay increase's, I suggested a ladder type progression 
system for all lab staff, similar to what some hospitals offer nursing staff.  
My question to this group is, if any of you are using such a system would you 
mind sharing it with me?

Thanks,

Stephen Clark  HT, BS, DESSO
Pathology Dept. Supervisor
Laboratory Safety Officer
Grand Strand Medical Center
809 82nd Parkway
Myrtle Beach, SC 29579
Desk: 843-692-1459
Lab: 843-692-1486
Fax: 843-449-6213

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[Histonet] Spill kits

[Anne Murvosh via Histonet]

Do spill kits need to be out where anyone can see them, or can I put it in a 
cupboard with a sign on the door?  I need to move mine and there's very little 
room.  Thanks Anne
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[Histonet] Hoods

[Anne Murvosh via Histonet]

We had an inspection from the fire department recently and they said we need to 
have the exhaust from the fume hood checked annually.  Does anyone know who 
does that?  The people that do our P.M. on our machines say they don't, so who 
would?  Thanks Anne
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Re: [Histonet] PAS Stain

[Anne Murvosh via Histonet]

You clearly don't know your histo history. The reason we know that H pylori 
exists is because a Scientist, Dr. Barry Marshall wanted to prove bacteria 
caused ulcers and not stress.  No one believed him.  So he took the organisms 
from a patient, mixed it in a broth and drank it. He then biopsied himself and 
treated it. There's a non-uniform method that saved a lot of suffering.  Bravo 
we crazy scientists.  Anne


-Original Message-
From: Geoff via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Thursday, May 05, 2016 12:31 PM
To: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] PAS Stain

I cannot believe any scientist would advocate such a non-uniform method 
as spitting on a slide.
Buy a bottle of what ever enzyme and use a reproducible buffer and 
temperature.

Geoff

On 5/5/2016 3:19 PM, Anne Murvosh via Histonet wrote:
> Yes, spitting is the tried and true way to do it.  Not to mention no 
> measuring and cheaper.  The reason we switched to a powder is because I just 
> don't spit well I used to have someone do it for me cause I would end up 
> drooling. YUCK! The best way to find out is do the amylase method and the 
> spit method at the same time and have the doctor pick the best.  A fun 
> experiment  Anne  
>
> -Original Message-
> From: Bob Richmond via Histonet [mailto:histonet@lists.utsouthwestern.edu]
> Sent: Thursday, May 05, 2016 11:36 AM
> To: koelli...@comcast.net
> Cc: Histonet@lists.utsouthwestern.edu
> Subject: Re: [Histonet] PAS Stain
>
> Spokane Ray points out something I've wondered about for years - can just
> anybody spit on the slide and remove the glycogen? I've never heard of any
> variation, but the number of people I've asked is very limited. This
> reference:
> http://www.ncbi.nlm.nih.gov/gene/276
> certainly suggests that different people have different salivary alpha
> amylase activity.
>
> Bob Richmond
>
> On Thu, May 5, 2016 at 2:27 PM, <koelli...@comcast.net> wrote:
>
>> I love having the Samuri Pathologist on this forum for wisdom and
>> real-laboratory life knowledge.  And yes, I have in the past spit on slide
>> ON OCCASSION when faced with a dire necessity.  Although I know there are
>> those who would wretch about this; it remains a fact of viable laboratory
>> life for some.
>>
>> My problem now is that in this era of (MUCH TOO MUCH) regulation, how do
>> you "test lots" or control from "lot-to-lot variation" in this SOP?  When
>> Jane or Joe do this routinely and then goes on vacation, what about Sally
>> or Jim spit?  There is a variation in copy number of the AMY1 gene
>> (amylase) and resulting difference in amylase protein concentration amongst
>> individuals.
>>
>> Why not just standardize it from the start, reagent, pH, temperature and
>> it really cannot fail.
>>
>> Spokane Ray
>>
>> --
>> *From: *"Bob Richmond via Histonet" <histonet@lists.utsouthwestern.edu>
>> *To: *"Histonet@lists.utsouthwestern.edu" <
>> histonet@lists.utsouthwestern.edu>
>> *Sent: *Thursday, May 5, 2016 11:10:40 AM
>> *Subject: *Re: [Histonet] PAS Stain
>>
>>
>> Amylase (diastase) for the PAS stain queries:
>>
>> Whatever happened to spitting on the slide (30 min at room temperature)?
>> John Kiernan advises "thinking of lemons and drooling into a small beaker"
>> though I'd advise chewing on a rubber band for a few seconds.
>>
>> He notes that alpha amylase is preferred. I'd go with the cheapest one in
>> the Sigma-Aldrich catalog. Room temperature is usual, but I note that Sigma
>> offers a heat-stable alpha amylase.
>>
>> Bob Richmond
>> Samurai Pathologist
>> Maryville TN
>> ___
>> Histonet mailing list
>> Histonet@lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>
>>
> ___
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>
> ___
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-- 
--
**
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732) 235-4583; fax: -4029
mcaul...@rwjms.rutgers.edu
**



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Re: [Histonet] PAS Stain

[Anne Murvosh via Histonet]

Yes, spitting is the tried and true way to do it.  Not to mention no measuring 
and cheaper.  The reason we switched to a powder is because I just don't spit 
well I used to have someone do it for me cause I would end up drooling. YUCK! 
The best way to find out is do the amylase method and the spit method at the 
same time and have the doctor pick the best.  A fun experiment  Anne

-Original Message-
From: Bob Richmond via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Thursday, May 05, 2016 11:36 AM
To: koelli...@comcast.net
Cc: Histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] PAS Stain

Spokane Ray points out something I've wondered about for years - can just
anybody spit on the slide and remove the glycogen? I've never heard of any
variation, but the number of people I've asked is very limited. This
reference:
http://www.ncbi.nlm.nih.gov/gene/276
certainly suggests that different people have different salivary alpha
amylase activity.

Bob Richmond

On Thu, May 5, 2016 at 2:27 PM,  wrote:

> I love having the Samuri Pathologist on this forum for wisdom and
> real-laboratory life knowledge.  And yes, I have in the past spit on slide
> ON OCCASSION when faced with a dire necessity.  Although I know there are
> those who would wretch about this; it remains a fact of viable laboratory
> life for some.
>
> My problem now is that in this era of (MUCH TOO MUCH) regulation, how do
> you "test lots" or control from "lot-to-lot variation" in this SOP?  When
> Jane or Joe do this routinely and then goes on vacation, what about Sally
> or Jim spit?  There is a variation in copy number of the AMY1 gene
> (amylase) and resulting difference in amylase protein concentration amongst
> individuals.
>
> Why not just standardize it from the start, reagent, pH, temperature and
> it really cannot fail.
>
> Spokane Ray
>
> --
> *From: *"Bob Richmond via Histonet" 
> *To: *"Histonet@lists.utsouthwestern.edu" <
> histonet@lists.utsouthwestern.edu>
> *Sent: *Thursday, May 5, 2016 11:10:40 AM
> *Subject: *Re: [Histonet] PAS Stain
>
>
> Amylase (diastase) for the PAS stain queries:
>
> Whatever happened to spitting on the slide (30 min at room temperature)?
> John Kiernan advises "thinking of lemons and drooling into a small beaker"
> though I'd advise chewing on a rubber band for a few seconds.
>
> He notes that alpha amylase is preferred. I'd go with the cheapest one in
> the Sigma-Aldrich catalog. Room temperature is usual, but I note that Sigma
> offers a heat-stable alpha amylase.
>
> Bob Richmond
> Samurai Pathologist
> Maryville TN
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
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[Histonet] Height regulations

[Anne Murvosh via Histonet]

Putting in some lower shelving for storage and I can't remember the height 
regulation for keeping stuff off the floor.  I tried to google it, it must be 
buried in some government doc.  Please Help?  Thanks Anne
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[Histonet] RE: NY State Histology License

[Anne Murvosh]

There is a grandfather clause if you were a histotech by april 24.1995.  I 
asked ClIA recently when they came to inspect me, as I fell into this category 
but didn't know if you had to have the licence by then or just have been 
performing high complexity test at that time (my licence was later than that).  
The CLIA person couldn't decipher their own rules and was going to ask her 
supervisor.  I never heard back.  As for IHC any HT in our state can do it and 
CAP never brought it up when I worked at the hospital.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Gail Marcella
Sent: Tuesday, April 21, 2015 8:48 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] NY State Histology License

Hi  - I've been a Histotech for 20+ years and got my Clinical Laboratory 
License in NY State when they required getting it. I don't have an Associates 
or Bachelor's degree but a Pathologist signed off for me. I have my HTASCP. I 
was told when I went for an interview in NY State that I couldn't gross small 
specimens or do IHC without an Associates  or Bachelor degree in biology. I was 
not aware of these restrictions. I don't see anything on the NYS website.  I 
was wondering if anyone else heard of this? Thanks - Gail
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RE: [Histonet] prevent wrinkles when cutting

[Anne Murvosh]

First make sure your block is cold.  Second, some models of microtome's and 
blades don't cut well without dulling the blade a bit.(newer models don't seem 
to need it.)  Lastly some processors don’t fix as well as others you may need 
to face the blocks in and soak them.  Again some places soak in ice water 
alone, some use fabric softener on the ice and some use ammonia water. (that 
one is stinky). Some places do soaking at all.  I now have a really old 
microtome and have to blow or breath on the paraffin lightly as I'm cutting, an 
old technique but effective.  Sorry its going to be a lot of trial and error.  
Maybe you can call the previous tech on how they did it there.  Anne

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rachel M 
Gonzalez
Sent: Monday, April 20, 2015 9:07 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] prevent wrinkles when cutting

Hi

Thursday was the first time I ever used a microtome I move to a lab
that does not have someone dedicated to cutting. I already miss her.

I have no problems getting ribbons of 10-30 sections long but the pieces
are half the size of the original block. I am guessing they are wrinkling.
What am I doing wrong?

Thanks
Rachel
Senior Scientist
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[Histonet] RE: cleaning glassware

[Anne Murvosh]

I always used to rinse the containers I used with alcohol and let them dry 
before doing the stain.  Some people use an acid alcohol rinse.  Anne

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jeff Halstead
Sent: Thursday, April 02, 2015 11:48 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] cleaning glassware

Hi, All-I am currently experiencing problems with our warthin-stary silver 
stain. The finished product has silver particulates all over the empty slide 
and deposited on the tissue making the stain very difficult to interpet. Any 
ideas would be very helpful.  thanx
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[Histonet] RE: Floor scraper

[Anne Murvosh]

just a paint scraper at your local hardware store. Some people attach it to a 
broom handle so they don't have bend down.  They may even have a long one.
Anne


From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] on behalf of Cates, Julia 
[julia.ca...@ahss.org]
Sent: Wednesday, March 4, 2015 10:53 AM
To: histonet-boun...@lists.utsouthwestern.edu; histonet@lists.utsouthwestern.edu
Subject: [Histonet] Floor scraper

Good Day Histonet!

I am wondering if anyone can recommend a tool/product to clean paraffin off 
linoleum floors.  We currently have a tool with a blade on it but it digs right 
into the flooring.  Any and all suggestion will be helpful.

Thanks,

Julia Cates, HT(ASCP)cm
Pathology Coordinator, Pathology
Florida Hospital Waterman
(352) 253- ext.4346 | Fax: (352) 253-3592

Confidentiality Statement: This email message, including any attachments, is 
for the sole use of the intended recipient and may contain confidential and 
privileged information. Any unauthorized review, use, disclosure, or 
distribution is prohibited.  If you are not the intended recipient, please 
contact the sender by reply to this email and delete the original and all 
copies of this email.


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[Histonet] RE: embedding

[Anne Murvosh]

My first job the molds were kept room temp or cool and I didn't have to scrape 
as much. My 2nd job they were kept hot and had to scrape all the time.  Have 
fun with it and test some cold, some room temp, some hot see if there is a 
difference. The problem with too cool molds is it takes a bit longer to embed 
cause the bottom takes longer to warm up so it's a trade off for time. Fun 
experiment though!  Anne 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Campbell, Tasha 
M.
Sent: Wednesday, January 21, 2015 7:12 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] embedding

Hello everyone, I was just curious if anyone had tips on how to embed without 
getting paraffin on the outside of the cassettes so I don't have to scrape the 
blocks or at least not scrape very much.  Thanks!!

 

 

 

 

Tasha Campbell, B.S.,HTL(ASCP)

Frederick Gastroenterology Associates

310 W. 9th St.

Frederick, MD 21701

301-695-6800 ext. 144 (w)

304-685-9307 (c)

 

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RE: [Histonet] marking small samples

[Anne Murvosh]

I mark shaves and punches with .5% Toludine blue it won't color the skin but 
will color the rest.  And best of all it doesn't show up in any staining its 
purely for identifying your tissue for embedding purposes.  Anne

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Yves Heremans
Sent: Wednesday, January 14, 2015 2:03 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] marking small samples

Dear Histonetters,

Does anyone know of a good method to mark (stain) small samples (tissue or 
cells) prior to paraffin embedding to aid in finding back more easily the 
sample in the paraffin block ?
Preferably something that would not interfere with subsequent antibody staining.

Yves


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[Histonet] RE: Animal Tissue/Human Tissue

[Anne Murvosh]

My first job was at a lab that did both human and animal (till a case got mixed 
up)  the only difference I found was animals were fattier and hairier.  Same 
microtome.  Anne

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Matthew 
Lauterbach
Sent: Tuesday, November 04, 2014 8:39 AM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] Animal Tissue/Human Tissue

Has anyone used the same microtome to cut animal tissue as well as human 
tissue?  We are a hospital clinical Histology lab being asked to provide 
research study slides from nonhuman sources.  Anyone have an input or 
precedence?  I couldn't find anything that specifically prohibits this practice 
from the CAP.  Thanks for the help in advance!!
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RE: [Histonet] Mohs

[Anne Murvosh]

On fatty tissue, especially cheeks. After we mount on a slide we spray the 
tissue with liquid nitrogen and let sit to freeze before embedding for a couple 
of minutes.  Than after embedding spray the chuck again with liquid nitrogen.  
On really fatty stuff you may have to freeze before cutting each time. Time 
consuming yes.  But I find freezing and let sitting I don't fight with it as 
much when cutting.  Anne

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of michele carr
Sent: Tuesday, September 23, 2014 9:00 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Mohs

Hello I perform mohs cutting a few days a week and I noticed that on fatty skin 
tissue I don't get the interior of the skin section. I only seem to get the 
epidermis. Is there a trick to getting the fatty tissue too. I tried bumping up 
the microns but that only helped a bit.
Thanks for your responses.

Michele Carr
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RE: [Histonet] Practice tissue?

[Anne Murvosh]

Contact your local labs and Hospitals, you could just use some already
embedded and cut tissue that is about to be disposed of as long as there
is no patient info on the blocks it shouldn't be a problem.  Anne

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of David
Waugh
Sent: Tuesday, June 03, 2014 2:51 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Practice tissue?

Hello histonet, I was a member a number of years ago, but had gotten out
of doing much histology (I was never a pro) for awhile but I seem to
be back at it. 
I was wondering if people might have some ideas on a good practice
tissue, one that I could embed, section, and stain (HE) to both
practice my sectioning, and check my staining protocol, etc. I really
need to be sectioning some decalcified teeth, and have had some luck,
but I would really like to do some more practice on an easy to section
tissue (other than just a blank block of paraffin)  that I can also
test my staining on.  I have accesses to a tissue processor. Is there
anything I can just pick up at the grocery store? Thanks, David
David A. Waugh, Ph.D.
Research Assistant
Department of Anatomy and Neurobiology
NEOMED
4209 State Route 44
Rootstown, OH 44272


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RE: [Histonet] Microtome

[Anne Murvosh]

Most companies will let you test run their newest microtomes. Let them
come to you and test for a week or so than pick the one you like best.
Anne

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sylvia
Shockey
Sent: Monday, June 02, 2014 2:20 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Microtome


Hello,
   My name is Sylvia Shockey I work in a histology lab.
One of our microtomes is not working properly and would appreciate some
advice on purchasing a new one or maybe refurbished.  I have had some
quotes on a Tanner scientific manual rotary microtome, titan 5000 .
Thermo Shandon Finesse and a Thermo Shandon Microm HM325. Mr. Grimm with
Thermo fisher has new and used on both of these models. Help me please I
don't want to buy a clunker.  Thanks for your time.





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RE: [Histonet] (no subject)

[Anne Murvosh]

I just got back from the Mohs conference.  The Biocare people have the stain 
and told me they would come out to help work it up. Anne

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bethany
Sent: Thursday, May 29, 2014 1:23 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] (no subject)

The doctor I work for wants to use Mart 1 staining for Mohs on melanoma cases.
Any information on where to start? What equipment do we need? Where do we 
acquire the stains/antibodies, etc?
thx
Bethany
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RE: [Histonet] antibody vials

[Anne Murvosh]

We got some plexiglass dividers at the Container Store if you have one
near or check online.  Can't remember what they were called but they had
different sized square compartments that fit various venders antibodies.
Anne

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Clare
Thornton
Sent: Tuesday, May 20, 2014 7:48 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] antibody vials

Does anyone have any good ideas for keeping the little vials of
concentrated antibodies neat and organized in the fridge?  Right now we
keep ours in empty pipette tip boxes (without tip holders) but they're
always falling over and mixed up.  We use several different vendors for
our concentrated antibodies, so our vials are all different sizes.
Thanks for any ideas!



Clare J. Thornton, HTL(ASCP)QIHC
Lead Immunohistochemistry Technologist
Dahl-Chase Diagnostic Services
417 State Street, Suite 540
Bangor, ME 04401
cthorn...@dahlchase.commailto:cthorn...@dahlchase.com


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RE: [Histonet] company for antibody HSV(cocktail) on paraffin section

[Anne Murvosh]

Biocare has a herpes III mix if that's what you're looking for. Anne

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
Pathology-Histology Sr. Supervisor
Sent: Monday, April 28, 2014 5:27 AM
To: histonet@lists.utsouthwestern.edu
Cc: tahseen0...@gmail.com
Subject: [Histonet] company for antibody HSV(cocktail) on paraffin
section

Hi All
We are looking company for antibody HSV(cocktail) on paraffin section,
kindly any comments.
Regards
Muhammad Tahseen
Histology supervisor
SKMCHRC
Lahore

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RE: [Histonet] Ventana Vantage and HE stainer

[Anne Murvosh]

For the most part I like this system.  A few things though.  Their HE
racks constantly break so make sure in your contract you get free ones
to replace them.  Also if you have to remove a coverslip it is a pain.
You have to heat the slide for a while so see if they will include a
heater and you will need it.  Every once and a while the coverslips get
a bad lot and you get bubbles.  Also the color of your cassettes can
make a big difference on if the barcode reader can pick up the code so
ask them about it. I don't work there any more so maybe they have fixed
things Sacred Heart Hospital in Spokane Wa Has the complete system so
you could give them a call. 509-474-4108  Thanks Anne

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Amber
McKenzie
Sent: Wednesday, February 12, 2014 10:50 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Ventana Vantage and HE stainer 


So, Ventana came out to my lab to do a LEAN consult for us since we are
building a new lab...they want to sell us their vantage tracking system
and HE stainer.  Our Rep is coming on Fri to talk to me, our lab
director and CEO about these products.  Anyone using these?  I'd like
some feedback so I can be prepared with questions on Fri for our
meeting.  Thanks!

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RE: [Histonet] Safety items

[Anne Murvosh]

Don't forget to make sure you have a spill kit and its labeled well with
easy access,  Inspectors will ask employees if they know where it and
your fire extinguisher and alarm are located. Anne

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Amber
McKenzie
Sent: Tuesday, January 07, 2014 6:35 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Safety items


What safety items do you keep in your lab besides the normal apron,
gloves, eye wash stations and shower?  Thanks!

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[Histonet] Paperwork

[Anne Murvosh]

OK, I have another CAP Vs CLIA question. Can we toss paperwork after 2
years with Clia, like you do CAP specifically Temperature and
maintenance charts.  Thanks Anne

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RE: [Histonet] Helicobacter helmani -- help?

[Anne Murvosh]

We had same problem at my old job.  I don't think they have come up with a 
separate IHc so our doctor would order an alcian yellow with ihc on children 
under age of 12 I think but we gave this up cause it's so rare. If you look up 
on Google under h. Heilmannii,  I believe its spelled, it has a lot of papers 
written on it that might help.  Anne

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cheryl
Sent: Wednesday, December 04, 2013 12:19 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Helicobacter helmani -- help?

Hi Guys-

Does the routine Helicobacter pylori IHC staining generally stain the whole 
spectrum of helicos?

We had a clinician ask specifically about the helmani and we're waiting for a 
response from the vendor but now I'm curious, too!

Thanks in advance and Wednesday--

Cheryl

Cheryl Kerry, HT(ASCP) 
Full Staff Inc. 
Staffing the AP Lab by helping one GREAT Tech at a time.  
281.852.9457 Office
800.756.3309 Phone  Fax 
ad...@fullstaff.org 

Sign up for the FREE newsletter AP News--updates, tricks of the trade and 
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RE: [Histonet] Rate of microtome injuries in Histotechnology schools.

[Anne Murvosh]

If anyone wants to cut down on cutting injuries.  Teach the new techs to
use two forceps instead of your fingers.  I'm too old to change but I
know a tech who cuts this way and has never had an injury.  It works
really well on automated microtomes.  Anne

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tom
Wells
Sent: Wednesday, November 27, 2013 12:11 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Rate of microtome injuries in Histotechnology
schools.

Hi,
I am curious about the rate of microtome cuts that students receive in
Histotechnology schools. We have a rather large program and take in
approximately 60 (or more)  students a year. Our Histotechnology
semester is around 20 weeks long. During that time our students cut
approximately 300 - 400 blocks per student. We typically have
approximately 10 minor cuts per semester. How does this compare to other
schools? Please email or call me directly to discuss this further. Thank
you very much. Tom

Tom Wells BSc, MEd, MLT, ART
Faculty
Department of Medical Laboratory Sciences, School of Health British
Columbia Institute of Technology SW03-3088, 3700 Willingdon Avenue
Burnaby, BC, V5G 3H2

E tom_we...@bcit.ca
T (604) 412-7594
C (778) 228 -4102 

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