[Histonet] text-books for sale

[Houston, Ronald via Histonet]

Happy Thanksgiving

My turn now; I have decided to retire mid-January and am starting to clear out 
my office.

I have the following text-books for sale. There will be a standard charge of 
$5.50 for shipping and handling (irrespective of how many books you wish)

A few books may have some highlighting, but the majority are in pristine 
condition.

Please contact me off-line at 
ronniehh1...@yahoo.com to order and for payment 
arrangements (will accept money order, check or PayPal)


Management& Business Improvement

The Idea Generator
Tozawa B, Bodek N
PCS Press 2001  $23.99
plus
The Idea Generator Workbook - Quick and Easy Kaizen
Tozawa B, Bodek N
PCS Press 2002

Leadership Without Excuses
Grimshaw J, Baron G
McGraw Hill 2008  $11.99

Difficult Conversations
Stone D, Patton B, Heen S
Penguin  2010  $5.99

Coping With Difficult People in the Health Care Setting
Umiker W
ASCP Press 1994 (free with purchase of any one other management/business 
improvement book)

Leadership and Self-Deception: Getting Out of the Box 2nd Edition
The Arbinger Institute
Berrett-Koehler Publishers 2015  $5.99

Why Leadership Sucks(TM): Fundamentals of Level 5 Leadership and Servant 
Leadership
Smith, MA, Wolf M
Kompelling Publishing 2012   $9.99

Managers Guide to Performance Reviews
(free with purchase of any one other management/business improvement book)

Beyond Heroes: A Lean Management System for Healthcare
Barnas K
ThedaCare Center for Healthcare Value 2012 $15.99

The Lean Turnaround: How Business Leaders Use Lean Principles to Create Value 
and Transform Their Company
Byrne A, Womack J
McGraw Hill 2012 $11.99

The Spirit of Kaizen
Maurer R
McGraw Hill 2012  $9.99

Gemba Kaizen: A Commonsense Approach to a Continuous Improvement Strategy, 2nd 
Edition
Imai M
McGraw Hill 2012  $11.99

The Pittsburgh Way to Efficient Healthcare
Grunden N
Healthcare Performance Press 2008  $16.25

Improving Healthcare Using Toyota Lean Production Methods, 2nd Edition
Chalice R
ASQ Press 2005   $8.50

Lean Thinking: Banish Waste and Create Wealth in your Corporation
Womack JP, Jones DT
Simon & Schuster 1996 (free with purchase of any one other management/business 
improvement book)

What is Lean Six Sigma?
George M, Rowlands D, Kastle B
McGraw-Hill 2004 (free with purchase of any one other management/business 
improvement book)

Lean Hospitals: Improving Quality, Patient Safety, and Employee Satisfaction
Graban M
CRC Press  2009  $6.75

Complete Idiot's Guide to Lean Six Sigma
Breakthrough Management Group  $5.25

Developing Lean Leaders at All Levels: A Practical Guide
Liker JK
Lean Leadership Institute Publications 2015  $21.75


Pathology & Medicine

Scientific Imaging with Photoshop: Methods, Measurement, and Output
Sedgewick J
New Riders 2008  $100.25

Bone Marrow Immunohistochemistry
Torlakovic EE, Naresh KN, Brunning RD
ASCP 2008  $92.15

Diagnostic Histochemistry (with CD-ROM)
Wick M
Cambridge University Press 2008  $82.99

Tumors of Haematopoietic and Lymphoid Tissues
Jaffe ES, Harris NL, Vardiman JW (eds)
WHO - IARC  $18.95

Pathology Informatics: Theory and Practice
Pantanowitz, L
ASCP 2012  $105.15

Practical Pathology Informatics: Demystifying informatics for the practicing 
anatomic pathologist
Sinard, J
Springer 2010  $30.55

Pathology of Chronic Constipation in Pediatric and Adult Coloproctology
Meier-Rouge WA, Bruder E
Karger  2005  $95.00

Schuster Atlas of Gastrointestinal Motility in Health and Disease(with CD-ROM)
Schuster  MM, Crowell MD, Koch KL
BC Decker  2002  $15.99

The Second Brain: A Groundbreaking New Understanding of Nervous Disorders of 
the Stomach and Intestine
Gershan MD
Harper 1999  $17.25

Atlas of Pediatric Brain Tumors
Adesina AM, Tihan T, Fuller CE, Poussaint TY
Springer 2016 $55.75

Clinical Neuropathology: Text and Color Atlas
Haberland C
Demos 2006  $61.25

Modern Immunohistochemistry (with DVD-ROM)
Chu P, Weiss L
Cambridge 2014 $120.25

A Practical Guide to Frozen Section Technique
Peters SR
Springer 2009  $88.99

Color Atlas of Immunocytochemistry in Diagnostic Cytology
Ganjel-Azar P, Nadji M
Springer 2006  $65.95

Diagnostic Electron Microscopy: A Practical Guide to Interpretation and 
Technique
Stirling J, Curry A, Eyden B
Wiley 2013  $70.00

Handbook of Practical Immunohistochemistry: Frequently Asked Questions
Lin F, Prichard J
Springer 2015  $135.15




Immunomicroscopy: A Diagnostic Tool for the Surgical Pathologist 3rd Edition
Taylor CR, Cote RJ
Saunders 2005  $73.15

The Enteric Nervous System
Furness JB
Wiley-Blackwell 2006  $80.35

The Falling Sickness: a History of Epilepsy from the Greeks to the Beginnings 
of Modern Neurology
Temkin, O
John Hopkins University Press 1994  $16.35

A Disease Once Sacred: a History of the Medical Understanding of Epilepsy
Eadie MJ, Bladin PF
John Libbey 2001  $39.95

Pathology of Peripheral Nerves
Weller RO, Cervos-Navarro J
Butterworths 1977  $27.99

Color Atlas of Cytology, Histology, and Microscopic Anatomy
Kuehnel W

[Histonet] Process Improvement and Quality Initiatives

[Houston, Ronald via Histonet]

We are in the process of re-examining what we discuss in our daily huddles with 
a view to involve all pathologists, and was wondering what others are doing in 
relation to topics for daily study and as part of a daily management system 
e.g.  Metrics and Goals;  Daily Readiness Assessment -MESA (Methods, Equipment, 
Supplies, Associates); Problem Management - ownership, expectations, 
countermeasures.

Do pathologists get actively involved in huddles in your facilities?

Any suggestions for getting all staff fully engaged?

Thanks for any help/suggestions
Ronnie

Ronnie Houston, MS HT(ASCP)QIHC FIBMS
Anatomic Pathology Manager
Laboratory Services
700 Children's Drive
Columbus, OH 43205
(P) 614-722-5450
(F) 614-722-2899
ronald.hous...@nationwidechildrens.org
www.NationwideChildrens.org

"Without continual growth and progress, such words as improvement, achievement, 
and success have no meaning."
~ Ben Franklin

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[Histonet] pediatric facilities only

[Houston, Ronald via Histonet]

If you do not work in a pediatric facility please disregard

I need some help getting statistics for benchmarking for staffing evaluation

What is your annual volume of cases/blocks?
Continual flow processing?
# of IHC/Special stains
EM?
Shifts?
Days only?
Weekend coverage?
How many HTs do you have?
How many PAs?
Lab Assistants?
How many pathologists?
Do you perform any research?
What do you do regarding transcription? # of transcriptionists, outsource, 
voice recognition?

Thanks
Ronnie

Ronnie Houston, MS HT(ASCP)QIHC FIBMS
Anatomic Pathology Manager
Laboratory Services
700 Children's Drive
Columbus, OH 43205
(P) 614-722-5450
(F) 614-722-2899
ronald.hous...@nationwidechildrens.org
www.NationwideChildrens.org

"Without continual growth and progress, such words as improvement, achievement, 
and success have no meaning."
~ Ben Franklin

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[Histonet] Caspase 3 and Tunel Assay

[Houston, Ronald via Histonet]

Is anyone working on this just now? And more specifically on the pig species?

Any pointers would be appreciated
Thanks

Ronnie Houston, MS HT(ASCP)QIHC FIBMS
Anatomic Pathology Manager
Laboratory Services
700 Children's Drive
Columbus, OH 43205
(P) 614-722-5450
(F) 614-722-2899
ronald.hous...@nationwidechildrens.org
www.NationwideChildrens.org

"Without continual growth and progress, such words as improvement, achievement, 
and success have no meaning."
~ Ben Franklin

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Re: [Histonet] Rachel Pinch, HT(ASCP)

[Houston, Ronald via Histonet]

Have to disagree with performing H.pylori testing on all gastric biopsies. It 
cannot be justified as best practice if there is no clinical or pathological 
indication.


Ronnie Houston, MS HT(ASCP)QIHC FIBMS
Anatomic Pathology Manager
Laboratory Services
Nationwide Chidlren's Hospital
700 Children's Drive
Columbus, OH 43205
(P) 614-722-5450
(F) 614-722-2899
ronald.hous...@nationwidechildrens.org
www.NationwideChildrens.org

"Without continual growth and progress, such words as improvement, achievement, 
and success have no meaning."
~ Ben Franklin



-Original Message-
From: Forest Blankenship via Histonet 
[mailto:histonet@lists.utsouthwestern.edu] 
Sent: Wednesday, June 22, 2016 11:20 AM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] Rachel Pinch, HT(ASCP)

Rachel; if you are talking about testing for H. Pylori on gastric samples it 
can be justified as a best practice. I was surprised by how many kids have that 
bacterium when I moved down to Corpus to work in a pediatric hospital. I do 
miss H-town and it's theater scene.

Best wishes,

F. Kevin Blankenship, HTL

Disclaimer: This email and its content are confidential and intended solely for 
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[Histonet] x-raying specimens

[Houston, Ronald via Histonet]

Curious to know how you are billing for any x-raying you may do on surgical and 
autopsy specimens?

Ronnie Houston, MS HT(ASCP)QIHC
Anatomic Pathology Manager
700 Children's Drive
Columbus, OH 43205
(P) 614-722-5450
(F) 614-722-2899
ronald.hous...@nationwidechildrens.org
www.NationwideChildrens.org

"Without continual growth and progress, such words as improvement, achievement, 
and success have no meaning."
~ Ben Franklin

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Re: [Histonet] DIF on paraffin embedded tissue

[Houston, Ronald via Histonet]

Obviously it depends on what you are looking at, but I have had much success 
with DIF on paraffin sections


Ronnie Houston, MS HT(ASCP)QIHC FIBMS
Anatomic Pathology Manager
700 Children's Drive
Columbus, OH 43205
(P) 614-722-5450
(F) 614-722-2899
ronald.hous...@nationwidechildrens.org
www.NationwideChildrens.org

"Without continual growth and progress, such words as improvement, achievement, 
and success have no meaning."
~ Ben Franklin



-Original Message-
From: Rene J Buesa via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Wednesday, November 25, 2015 11:02 AM
To: madeathri...@pastnashville.com; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] DIF on paraffin embedded tissue

No matter WHO to tell you to do WHAT, for IF purposes, that FFPE tissue is 
USELESS.René 


On Wednesday, November 25, 2015 10:55 AM, Maryann Deathridge via Histonet 
 wrote:
 

 We have a tissue sample that was processed and paraffin embedded.  We URGENTLY 
need to recover the tissue and perform Immunofluorescence on the sample.
 Does anyone have a procedure.  HELP
  
    madeathri...@pastnashville.com  

  




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[Histonet] MAP2K7 IHC

[Houston, Ronald via Histonet]

Does anyone do MAP2K7 immunohistochemistry testing with interpretation on 
clinical specimens? We have a neuroblastoma that our oncologists want tested.
Thanks

Ronnie Houston, MS HT(ASCP)QIHC
Anatomic Pathology Manager
700 Children's Drive
Columbus, OH 43205
(P) 614-722-5450
(F) 614-722-2899
ronald.hous...@nationwidechildrens.org
www.NationwideChildrens.org

"Without continual growth and progress, such words as improvement, achievement, 
and success have no meaning."
~ Ben Franklin

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[Histonet] hepatocellular carcinoma

[Houston, Ronald via Histonet]

As I didn't get any bites yesterday, let me try another approach.

Can anyone help with a couple of unstained sections from hepatocellular 
carcinoma?

We are investigating an antibody that we hope will help in differentiating 
hepatoblastoma (a rare malignant tumor of the liver in children) from 
hepatocellular carcinoma. We have more than enough hepatoblastoma cases, but 
few HCC

Thanks
Ronnie

Ronnie Houston, MS HT(ASCP)QIHC FIBMS
Anatomic Pathology Manager
ChildLab, a Division of Nationwide Children's Hospital
www.childlab.com

700 Children's Drive
Columbus, OH 43205
(P) 614-722-5450
(F) 614-722-2899
ronald.hous...@nationwidechildrens.orgmailto:ronald.hous...@nationwidechildrens.org
www.NationwideChildrens.orghttp://www.nationwidechildrens.org/

One person with passion is better than forty people merely interested.
~ E.M. Forster

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[Histonet] hepatocellular carcinoma

[Houston, Ronald via Histonet]

Is anyone able to spare a block of hepatocellular carcinoma?

Can swop with CMV, Adenovirus, RSV control blocks?
Thanks


Ronnie Houston, MS HT(ASCP)QIHC FIBMS
Anatomic Pathology Manager
ChildLab, a Division of Nationwide Children's Hospital
www.childlab.com

700 Children's Drive
Columbus, OH 43205
(P) 614-722-5450
(F) 614-722-2899
ronald.hous...@nationwidechildrens.orgmailto:ronald.hous...@nationwidechildrens.org
www.NationwideChildrens.orghttp://www.nationwidechildrens.org/

One person with passion is better than forty people merely interested.
~ E.M. Forster

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Re: [Histonet] Film Coverslip

[Houston, Ronald via Histonet]

At previous place of employment we used Statlab's tape - absolutely no problems 
with stain bleeding or fading, bubbles or peeling of the tape. There were no 
problems with the coverslipper either.

Of course Sakura is going to warn against using any other product but theirs. 
Consumables are how they stay in business.
Ronnie

-Original Message-
From: Rene J Buesa via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Friday, July 24, 2015 9:50 AM
To: Michael Kent; Histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Film Coverslip

If you are using the Sakura instrument, please do not use other film than 
Sakura's. It is not only much better but also will allow the coverslipper to 
work better.Sometimes a cheaper option will be more costly at the end.René 


 On Friday, July 24, 2015 9:47 AM, Michael Kent via Histonet 
histonet@lists.utsouthwestern.edu wrote:
   

 Good morning, We are considering changing coverslip film for our high volume 
Sakura Prisma with coverslipper.  We have tested StatLab film and pathologists 
are fine, and have not seen media build up on the instrument.
Sakura is cautioning against alternatives for lamination and media build-up 
issues.  Any feedback from Histonetters will be appreciated.
Best and have a great weekend,
Mike
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[Histonet] Lynch syndrome

[Houston, Ronald]

I know it is a long-shot, but does anyone know of a TMA made with known Lynch 
Syndrome cases?

We have been asked to validate MSI antibodies for evaluating CMMRD 
(Constitutional Mismatch Repair Deficiency) in pediatric brain tumors and don't 
have access to Lynch Syndrome in our pediatric facility

Any help appreciated

Thanks
Ronnie

Ronnie Houston, MS HT(ASCP)QIHC
Anatomic Pathology Manager
ChildLab, a Division of Nationwide Children's Hospital
www.childlab.com

700 Children's Drive
Columbus, OH 43205
(P) 614-722-5450
(F) 614-722-2899
ronald.hous...@nationwidechildrens.orgmailto:ronald.hous...@nationwidechildrens.org
www.NationwideChildrens.orghttp://www.nationwidechildrens.org/

One person with passion is better than forty people merely interested.
~ E.M. Forster

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[Histonet] HT vacancy

[Houston, Ronald]

We have an immediate opening for a  registered HT. This would be a great 
opening for a newly registered tech as you would have the opportunity to learn 
and perform all aspects of histotechnology, from routine microtomy to electron 
microscopy, from special stains to IHC/ISH/IF and enzyme histochemistry.

Please contact me for more information and go to our web-site to apply 
(https://careers.nationwidechildrens.org) and to see our incredible new 
facility (http://www.nationwidechildrens.org/)


Come work for an organization where everything matters


Ronnie Houston, MS HT(ASCP)QIHC
Anatomic Pathology Manager
ChildLab, a Division of Nationwide Children's Hospital
www.childlab.com

700 Children's Drive
Columbus, OH 43205
(P) 614-722-5450
(F) 614-722-2899
ronald.hous...@nationwidechildrens.orgmailto:ronald.hous...@nationwidechildrens.org
www.NationwideChildrens.orghttp://www.nationwidechildrens.org/

One person with passion is better than forty people merely interested.
~ E.M. Forster

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RE: [Histonet] Frozen Sections

[Houston, Ronald]

we do, during the day Monday - Friday (8:00-17:30) but not over the weekend

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mike Pence
Sent: Monday, February 09, 2015 9:05 AM
To: 'lsmal...@juno.com'; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Frozen Sections

We provide assistance while the dept. is staff with histology techs.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of 
lsmal...@juno.com
Sent: Friday, February 06, 2015 9:41 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Frozen Sections

Hi, I need to ask Histoland a questionHow many HT departments provide 
assistance to the pathologist in the performance of frozen sections (cutting 
and staining of slides) to be evaluated by the pathologist?   Thank you very 
much in advance!  
Lorraine


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[Histonet] VEGF3 and VEGF

[Houston, Ronald]

Can you please tell me which antibody sources you have had best results for 
these epitopes?

we've been getting inconsistent results with the ones we've tried
Thanks
Ronnie

Ronnie Houston, MS HT(ASCP)QIHC
Anatomic Pathology Manager
ChildLab, a Division of Nationwide Children's Hospital
www.childlab.com

700 Children's Drive
Columbus, OH 43205
(P) 614-722-5450
(F) 614-722-2899
ronald.hous...@nationwidechildrens.orgmailto:ronald.hous...@nationwidechildrens.org
www.NationwideChildrens.orghttp://www.nationwidechildrens.org/

One person with passion is better than forty people merely interested.
~ E.M. Forster

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[Histonet] old journal article

[Houston, Ronald]

Does anyone have access to old archives of Am J Clin Pathol from 1985?

Looking for Sarnat, H.B., et al.:  A fluorochromic stain for nuclei 
acids to demonstrate submucosal and myenteric neurons in Hirschsprungs disease. 
 Amer. J. Clin. Path. 83:722-725, 1985

On-line access only goes back to 2000

Thanks
Ronnie

Ronnie Houston, MS HT(ASCP)QIHC
Anatomic Pathology Manager
ChildLab, a Division of Nationwide Children's Hospital
www.childlab.com

700 Children's Drive
Columbus, OH 43205
(P) 614-722-5450
(F) 614-722-2899
ronald.hous...@nationwidechildrens.orgmailto:ronald.hous...@nationwidechildrens.org
www.NationwideChildrens.orghttp://www.nationwidechildrens.org/

One person with passion is better than forty people merely interested.
~ E.M. Forster

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[Histonet] RE: specials stainers

[Houston, Ronald]

DAKO wins hands-down, without any reservation

Ronnie Houston, MS HT(ASCP)QIHC FIBMS
Anatomic Pathology Manager
ChildLab, a Division of Nationwide Children's Hospital
www.childlab.com

700 Children's Drive
Columbus, OH 43205
(P) 614-722-5450
(F) 614-722-2899
ronald.hous...@nationwidechildrens.org
www.NationwideChildrens.org

One person with passion is better than forty people merely interested.
~ E.M. Forster



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mitchell, 
Janice A
Sent: Tuesday, January 20, 2015 6:43 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] specials stainers

Good Morning,
We are looking for an automatic stainer for special stains.   Ventana vs Dako?  
Thoughts?
Thanks for any input, Janice Mitchell
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[Histonet] TCR immunohistochemistry

[Houston, Ronald]

has anyone had any success with the antibodies available for TCR lymphocytes in 
human paraffin tissue?
Thanks
Ronnie

Ronnie Houston, MS HT(ASCP)QIHC
Anatomic Pathology Manager
ChildLab, a Division of Nationwide Children's Hospital
www.childlab.com

700 Children's Drive
Columbus, OH 43205
(P) 614-722-5450
(F) 614-722-2899
ronald.hous...@nationwidechildrens.orgmailto:ronald.hous...@nationwidechildrens.org
www.NationwideChildrens.orghttp://www.nationwidechildrens.org/

One person with passion is better than forty people merely interested.
~ E.M. Forster

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[Histonet] melanoma control

[Houston, Ronald]

Anyone have a spare Melanoma control block (something positive for HMB-45, 
Melan-A and Tyrosinase)?

Can trade for something you may need. We have a ton of C3d/C4d positive 
rejected kidney blocks

Ronnie Houston, MS HT(ASCP)QIHC
Anatomic Pathology Manager
ChildLab, a Division of Nationwide Children's Hospital
www.childlab.com

700 Children's Drive
Columbus, OH 43205
(P) 614-722-5450
(F) 614-722-2899
ronald.hous...@nationwidechildrens.orgmailto:ronald.hous...@nationwidechildrens.org
www.NationwideChildrens.orghttp://www.nationwidechildrens.org/

One person with passion is better than forty people merely interested.
~ E.M. Forster

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[Histonet] IF on paraffin

[Houston, Ronald]

Anyone doing this? Needs some pointers in balancing quenching autofluorescence 
and suppression of fluorescent signal

Thanks

Ronnie Houston, MS HT(ASCP)QIHC
Anatomic Pathology Manager
ChildLab, a Division of Nationwide Children's Hospital
www.childlab.com

700 Children's Drive
Columbus, OH 43205
(P) 614-722-5450
(F) 614-722-2899
ronald.hous...@nationwidechildrens.orgmailto:ronald.hous...@nationwidechildrens.org
www.NationwideChildrens.orghttp://www.nationwidechildrens.org/

One person with passion is better than forty people merely interested.
~ E.M. Forster

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[Histonet] Dragon Voice Recognition software

[Houston, Ronald]

Is anyone using this transcribing directly into Sunquest CoPathPlus?

How successful was the transition, what problems did you encounter, and what 
resistance if any was there from pathologists, PAs and transcriptionists?

Thanks

Ronnie Houston, MS HT(ASCP)QIHC FIBMS
Anatomic Pathology Manager
ChildLab, a Division of Nationwide Children's Hospital
www.childlab.com

700 Children's Drive
Columbus, OH 43205
(P) 614-722-5450
(F) 614-722-2899
ronald.hous...@nationwidechildrens.orgmailto:ronald.hous...@nationwidechildrens.org
www.NationwideChildrens.orghttp://www.nationwidechildrens.org/

One person with passion is better than forty people merely interested.
~ E.M. Forster


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[Histonet] new IHC billing and Sunquest CoPathPlus

[Houston, Ronald]

Besides manually entering IHC charges, has anyone worked out a way to 
automatically bill for 88342 and 88343 on cases in Sunquest CoPathPlus?

Thanks

Ronnie Houston, MS HT(ASCP)QIHC
Anatomic Pathology Manager
ChildLab, a Division of Nationwide Children's Hospital
www.childlab.com

700 Children's Drive
Columbus, OH 43205
(P) 614-722-5450
(F) 614-722-2899
ronald.hous...@nationwidechildrens.orgmailto:ronald.hous...@nationwidechildrens.org
www.NationwideChildrens.orghttp://www.nationwidechildrens.org/

One person with passion is better than forty people merely interested.
~ E.M. Forster

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[Histonet] Specialist II position opening

[Houston, Ronald]

Due to growth, Nationwide Children's Hospital, Columbus, Ohio is pleased to 
open a new position for a Specialist II in the Department of Anatomic Pathology.

We are looking for an HT/HTL or MT, with at least a BS and a minimum of eight 
(8) years experience in all aspects of histotechnology in a clinical and/or 
research laboratory.

The Specilaist II is responsible for overseeing and maintaining the technical 
and procedural operations of the department; researching, developing and 
implementing new or improved methodologies and instrumentation; performing and 
monitoring quality assurance, quality improvement and regulatory compliance 
tasks; and will directly report to and support the anatomic pathology manager.

You will have the opportunity to work not only in one of the top pediatric 
facilities in the world but also, we believe, one of the premier anatomic 
pathology departments. We are a forward thinking department determined to 
achieve the best outcomes for our children, constantly seeking to adapt and 
improve the services we provide, and increasing the diagnostic, prognostic and 
predictive testing available.

Nationwide Children's is a place where staff are fully engaged, and a place 
that the staff are truly proud to be a part of..

We invite suitable candidtaes to check us out and apply online at 
http://nationwidechildrens.org and you can find out more about the outstanding 
care our kids get at http://700childrens.nationwidechildrens.org/

For more information about the position, please contact Ronnie Houston at 
ronald.hous...@nationwidechildrens.org and/or (614) histonet7225450




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[Histonet] Alport's testing

[Houston, Ronald]

Does anyone test for Alport's (alpha 1, 3  5 chains of collagen IV) on 
paraffin sections?

Ronnie Houston, MS HT(ASCP)QIHC
Anatomic Pathology Manager
ChildLab, a Division of Nationwide Children's Hospital
www.childlab.com

700 Children's Drive
Columbus, OH 43205
(P) 614-722-5450
(F) 614-722-2899
ronald.hous...@nationwidechildrens.orgmailto:ronald.hous...@nationwidechildrens.org
www.NationwideChildrens.orghttp://www.nationwidechildrens.org/

One person with passion is better than forty people merely interested.
~ E.M. Forster

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[Histonet] rare testing

[Houston, Ronald]

Looking for a lab(s) that can perform any or all of the following:

IHC for nuclear estradiol Beta R,  MYC-N,  VDR (vitamin D receptor), PDGFR, 
EGFR, VEGFR, IL-4R

Gene expression testing: MET, IGF, BAX (to determine sensitivity to Adriamycin 
and doxorubicin) and p53

Thanks
Ronnie

Ronnie Houston, MS HT(ASCP)QIHC
Anatomic Pathology Manager
ChildLab, a Division of Nationwide Children's Hospital
www.childlab.com

700 Children's Drive
Columbus, OH 43205
(P) 614-722-5450
(F) 614-722-2899
ronald.hous...@nationwidechildrens.orgmailto:ronald.hous...@nationwidechildrens.org
www.NationwideChildrens.orghttp://www.nationwidechildrens.org/

One person with passion is better than forty people merely interested.
~ E.M. Forster

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[Histonet] competency form

[Houston, Ronald]

Can someone please share the competency form(s) they are using to satisfy CAP?

I am having problems convincing our QA/Compliance folks of the differences 
between testing in AP compared to the other lab disciplines, who do read our 
test results

Thanks

Ronnie Houston, MS HT(ASCP)QIHC
Anatomic Pathology Manager
ChildLab, a Division of Nationwide Children's Hospital
www.childlab.com

700 Children's Drive
Columbus, OH 43205
(P) 614-722-5450
(F) 614-722-2899
ronald.hous...@nationwidechildrens.orgmailto:ronald.hous...@nationwidechildrens.org
www.NationwideChildrens.orghttp://www.nationwidechildrens.org/

One person with passion is better than forty people merely interested.
~ E.M. Forster

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[Histonet] RE: TAT for Transplant Biopsies

[Houston, Ronald]

Richard,



being a peds hospital, we are somewhat similar to Tony in Australia. If the 
clinician needs the results that day, that's what he gets (they don't abuse 
this either!). If on a Friday (or preceding a holiday), and they do not need 
the result that same day, we will process overnight and someone will come in 
and cut and stain the bx the following day.



To date, we have not had to come in on a Sunday, but someone is on-call for 
this (and other emergencies).



We do not use microwaves, but have great success using the Leica Peloris on a 1 
hour cycle, after a minimum of 30 minutes fixation.



We will not rush the special stains or IHC (C3d and C4d), but our regular 
cut-off time for those are 3:00pm for same day TAT and 4:30 for overnight IHC

Ronnie




Ronnie Houston, MS HT(ASCP)QIHC
Anatomic Pathology Manager
ChildLab, a Division of Nationwide Children's Hospital
www.childlab.com

700 Children's Drive
Columbus, OH 43205
(P) 614-722-5450
(F) 614-722-2899
ronald.hous...@nationwidechildrens.orgmailto:ronald.hous...@nationwidechildrens.org
www.NationwideChildrens.orghttp://www.nationwidechildrens.org/

One person with passion is better than forty people merely interested.
~ E.M. Forster



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cartun, Richard
Sent: Thursday, January 09, 2014 7:47 PM
To: Histonet
Subject: [Histonet] TAT for Transplant Biopsies



For those of you seeing biopsies of transplant kidney, liver, and heart, what 
is your cut-off time for accepting a specimen for same-day processing?  Do any 
of you handle specimens at night, weekends, or holidays?  Thank you!







Richard







Richard W. Cartun, MS, PhD



Director, Histology  Immunopathology



Director, Biospecimen Collection Programs



Assistant Director, Anatomic Pathology



Hartford Hospital



80 Seymour Street



Hartford, CT  06102



(860) 972-1596 Office



(860) 545-2204 Fax



richard.car...@hhchealth.orgmailto:richard.car...@hhchealth.orgmailto:richard.car...@hhchealth.org%3cmailto:richard.car...@hhchealth.org



This e-mail message, including any attachments, is for the sole use of the 
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are not the intended recipient, or an employee or agent responsible for 
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[Histonet] Bartonella PCR

[Houston, Ronald]

Does anyone know where we can get Bartonella PCR done on formaldehyde fixed, 
paraffin-embedded tissue?

Thanks

Ronnie Houston, MS HT(ASCP)QIHC
Anatomic Pathology Manager
ChildLab, a Division of Nationwide Children's Hospital
www.childlab.com

700 Children's Drive
Columbus, OH 43205
(P) 614-722-5450
(F) 614-722-2899
ronald.hous...@nationwidechildrens.orgmailto:ronald.hous...@nationwidechildrens.org
www.NationwideChildrens.orghttp://www.nationwidechildrens.org/

One person with passion is better than forty people merely interested.
~ E.M. Forster

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[Histonet] Endogen

[Houston, Ronald]

Does anyone know if Endogen has gone out of business or have they been gobbled 
up by another larger conglomerate? Trying to get one of their antibodies and 
can no longer find their web-site


Ronnie Houston, MS HT(ASCP)QIHC
Anatomic Pathology Manager
ChildLab, a Division of Nationwide Children's Hospital
www.childlab.com

700 Children's Drive
Columbus, OH 43205
(P) 614-722-5450
(F) 614-722-2899
ronald.hous...@nationwidechildrens.orgmailto:ronald.hous...@nationwidechildrens.org
www.NationwideChildrens.orghttp://www.nationwidechildrens.org/


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RE: [Histonet] Dimwits

[Houston, Ronald]

Time to log off Histonet again - stop acting like children and be the 
professionals you are (or should be). And you wonder why we get no 
respect???

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Marcum, Pamela A
Sent: Friday, September 13, 2013 10:32 AM
To: 'Tim Higgins'; anni...@gmail.com; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Dimwits

Thank you and well said!! 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tim Higgins
Sent: Friday, September 13, 2013 9:17 AM
To: anni...@gmail.com; histonet@lists.utsouthwestern.edu
Subject: [Histonet] Dimwits

Hello Annieinarabia,
 
You are quite the advocate for Sir Rene J. of the most respected voices on this 
forum.
 
Truly if you want to be respected, give respect and do not degrade other as he 
does from time to time (in my opinion).  Give substance in your responses and 
not comment using some pompous response to a valid question.  Like I said to 
someone off line, its not that he doesn't have knowledge (so I here, don't know 
him personally) to answer with useful creditable answers just at time he 
doesn't. I am sure everyone appreciates his sharing of knowledge, just be 
constructive in your responses.
 
I don't comment on a subject unless I have useful information to share, never 
to belittle the person asking who is in search of a solution to a problem.  
Take that approach and less people will be annoyed with the responses.
 
FYI, there is only one dimwit attached to this response unless your suggesting 
everyone on Histonet are dimwits.  Also, not sure why you are attacking the 
youngsters on this forum, this forum is for the young, old and middle-aged.
 
Have a fantastic Friday
 
TiminTexas not Tim M. (Don't want anyone mad at him on accident)
 
Message: 13
Date: Fri, 13 Sep 2013 09:52:45 +0400
From: Anne anni...@gmail.com
Subject: [Histonet] Re: Histonet Digest, Vol 118, Issue 24
To: histonet@lists.utsouthwestern.edu
histonet@lists.utsouthwestern.edu
Message-ID: ab6084bc-fab0-44f0-8cc1-0d1c6f141...@gmail.com
Content-Type: text/plain;   charset=utf-8

My goodness ignorance abounds...Rene J is one of the most respected voices on 
this forum.
And 'she' is actually a 'he' you dimwits. 
I would take his advice above almost anyone on this forum. 
You youngsters should try to emulate rather than denigrate. 
Rene you rock!!!

Annieinarabia 



  
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[Histonet] DNA ploidy

[Houston, Ronald]

Would anyone doing DNA ploidy be willing to share their protocol? It's a long 
time since I was involved in this and I'm sure there have been many advances
Thanks

Ronnie Houston, MS HT(ASCP)QIHC
Anatomic Pathology Manager
ChildLab, a Division of Nationwide Children's Hospital
www.childlab.com

700 Children's Drive
Columbus, OH 43205
(P) 614-722-5450
(F) 614-722-2899
ronald.hous...@nationwidechildrens.orgmailto:ronald.hous...@nationwidechildrens.org
www.NationwideChildrens.orghttp://www.nationwidechildrens.org/


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RE: [Histonet] IHC after EDTA decalcifying

[Houston, Ronald]

Perhaps I'm missing something here too. Did you decalcify the tonsil for the 
same length of time as the mouse femur? If you are looking at  e.g. CD markers 
in bone or indeed bone marrow, it is important that you have positive control 
material that you know works with undecalcified tissue and a corresponding 
piece of tissue that is treated in the same way as your test sample (i.e. 2 
tonsil samples from the same case, 1 of which has been decalcified and the 
other not, in the same control block - we also have bone marrow control blocks 
that we do the same with)

It is too much of a generalization that EDTA preserves all antigen sites. Some 
are affected much more than others. 
So to be able to help at all, what specific markers are you looking at, what 
are they raised in, and how are you detecting the antibody-antigen reaction?

and I completely agree with what Ray says also

Ronnie

Ronnie Houston, MS HT(ASCP)QIHC FRMS
Anatomic Pathology Manager
ChildLab, a Division of Nationwide Children's Hospital
www.childlab.com

700 Children's Drive
Columbus, OH 43205
(P) 614-722-5450
(F) 614-722-2899
ronald.hous...@nationwidechildrens.org
www.NationwideChildrens.org



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of 
koelli...@comcast.net
Sent: Thursday, August 15, 2013 10:17 PM
To: Colleen Forster
Cc: Histonet
Subject: Re: [Histonet] IHC after EDTA decalcifying



Could I ask exactly what you are looking for in mouse femur and human tonsil 
the tonsil  I assume is a control?  For what?  Whatever you are looking for is 
obviously in human tonsil.  Are you positive it is in murine femur.  I used to 
stain for 15-20 targets in EDTA decaled mouse femurs but my control was also a 
mouse. 



Ray 

Research Scientist 

Comparative Medicine 

University of Washington 


- Original Message -


From: Colleen Forster cfors...@umn.edu
To: Histonet histonet@lists.utsouthwestern.edu
Sent: Thursday, August 15, 2013 4:58:35 PM
Subject: [Histonet] IHC after EDTA decalcifying 

Hello fellow histonetters, 

Can someone who has worked with decalcifyied bone tell me if EDTA interferes 
with IHC staining? I was under the impression it did not but cannot get 
staining in mouse femurs that I have decaled in EDTA. I have used both the 
steamer and the decloaker for retrieval and in the human tonsil the stain is 
great. 

Any suggestions.. 

Thanks in advance! 

Colleen Forster
U of MN 

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[Histonet] RE: Transcriptionist Productivity (Willis, Donna G.)

[Houston, Ronald]

Beth
surely not 160 characters per hour; perhaps 160 lines/hour? This is one of the 
measurements of medical transcription productivity standards, typically rates 
fall within the 100-200 lines/hour, based on 65 characters/line, and depending 
on the speciality, type of facility and the dictator! 
The other major standard being Accuracy with a goal of ~98%.
My pet hamster could do more than 160 characters per hour and he's arthritic 
(accuracy probably down in the 0.98% range though)!
Ronnie

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of 
beth@hcahealthcare.com
Sent: Thursday, July 25, 2013 4:29 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Transcriptionist Productivity (Willis, Donna G.)

We have two types of transcriptionists working for our Market Pathology 
departments.  There are onsite transcriptionists that transcribe and have many 
other duties such as doing send outs and answering phones.  Because they have 
other miscellaneous duties, coming up with a standard was difficult, but we did 
do it.  We compare their characters typed by hour worked.  I add up all the 
character totals and then divide it between the number of transcriptionists.  
This is the average.  Each transcriptionist must be doing at least 80% of the 
average.  This helps to make sure that one transcriptionist is not doing less 
than others.

We also have transcriptionists that do transcription from home.  Since they do 
not have the interruptions or other duties, they have stricter guidelines.  The 
minimum that they are supposed to complete is 160 characters per hour worked 
however they often do much more.

I hope this helps, it's not an easy thing to keep track of, I know.  Let me 
know if you need any more information.

Beth A. Fye, CT (ASCP)
Pathology Technical Manager
HCA Richmond Hospital Laboratories
office:  (804) 228-6564
fax:  (877)699-3895
mailto:beth@hcahealthcare.com

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[Histonet] RE: IHC Elastin stain

[Houston, Ronald]

Linda,
we do it here


Ronnie Houston, MS HT(ASCP)QIHC
Anatomic Pathology Manager
ChildLab, a Division of Nationwide Children's Hospital
www.childlab.com

700 Children's Drive
Columbus, OH 43205
(P) 614-722-5450
(F) 614-722-2899
ronald.hous...@nationwidechildrens.org
www.NationwideChildrens.org

One person with passion is better than forty people merely interested.
~ E.M. Forster



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sebree Linda A
Sent: Tuesday, July 02, 2013 12:00 PM
To: Histonet (Histonet@lists.utsouthwestern.edu)
Subject: [Histonet] IHC Elastin stain

Do any of you do this?  Do any referral labs offer this?

Thanks,

Linda A. Sebree
University of Wisconsin Hospital  Clinics IHC/ISH Laboratory
600 Highland Ave.
Madison, WI 53792

(608)265-6596
FAX: (608)262-7174


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RE: [Histonet] HPV ISH Probes

[Houston, Ronald]

Many people are working on the Enzo probes just now. Mayo does have the probes 
up and running on the Bonds, but are keeping tight-lipped about their protocols.
We tried the Dako probes but never could get consistent results.

Ronnie Houston MS HT(ASCP)QIHC 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of joelle weaver
Sent: Tuesday, June 25, 2013 3:34 PM
To: Susan Foreman; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] HPV ISH Probes

HPV6, 11, 16 18, 31, 33  51 screening' 




Joelle Weaver MAOM, HTL (ASCP) QIHC
 
 From: sfore...@labpath.com
 To: histonet@lists.utsouthwestern.edu
 Date: Tue, 25 Jun 2013 14:10:59 -0400
 Subject: [Histonet] HPV ISH Probes
 
 Does anyone have HPV-ISH working?  utilizing a vendor for the probe 
 other than Ventana?  Prep Kit?
 
  
 
  
 
 Many Thanks,
 
 Susan Foreman, HT (ASCP)
 (865)584-1933
 
  
 
  
 
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[Histonet] RE: Her-2 antibody

[Houston, Ronald]

Never had any such problems with either the Max or the Bond IIIs in the 6+ 
years we have had them(no matter how many labels are on the slide - we have had 
three labels on a slide with no problems at all), unless of course your tissue 
extends to the rim of the covertile - then what would you expect?
You certainly do not need to have all 10 spaces filled on the tray. 
Suggest you contact Leica service as it seems as though your machine needs 
calibrated and realigned

Ronnie Houston, MS HT(ASCP)QIHC
Anatomic Pathology Manager
ChildLab, a Division of Nationwide Children's Hospital
www.childlab.com

700 Children's Drive
Columbus, OH 43205
(P) 614-722-5450
(F) 614-722-2899
ronald.hous...@nationwidechildrens.org
www.NationwideChildrens.org

One person with passion is better than forty people merely interested.
~ E.M. Forster



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sue Hunter
Sent: Wednesday, June 12, 2013 10:42 AM
To: Robert Fauck [CCDHB]; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Her-2 antibody

We have a Bond Max and run mainly ER/PR and ISH stains on that.  After Leica 
loaded in a new version of software in October we started having problems like 
that too.  Seems you have to have all 10 spots on the tray filled with a slide 
- doesn't have to be programed, just a slide and cover tile for the weight.  We 
also were doing a workaround with a Vantage label under the Leica label and 
found out that the two label thickness interferes with the movement of the 
cover tile (sometimes...but not always), which was affecting our staining.  
Even a regular slide label under the Leica label will cause a problem.  We 
finally have gone to entering a case, printing the labels but not putting them 
on the slides, cutting the sections and then putting the labels on the slide 
for positive patient identification.  Not ideal, and I worry a lot about case 
mix ups, but that is what is working to get consistent staining here.  As they 
say, The Enemy of Good is Better.  Wish Leica had not made it better!  It was 
working very nicely before.
Sue

Sue Hunter, Supervisor
Advanced Diagnostics
Beaumont Health System
Royal Oak MI
248-898-5146
shun...@beaumont.edu



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Robert Fauck 
[CCDHB]
Sent: Tuesday, June 11, 2013 1:05 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Her-2 antibody

Hi All
 
We here in wellington Hospital New Zealand having some problem with the
Her-2 ( Lieca, NCL-L-CBE-356 )
 
Top half of the slide ( control or Test tissue) are strongly positive but if we 
put the control tissue on the bottom half it is week to negative!
 
Does any one has the same problems with your Her-2, it is done on a Leica Bond 
(Max) III.
 
Much appreciated for any suggestions, also from Leica Technical specialists.
 
Regards,
Robert Fauck
Wellington hospital, NZ


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(1C_S1)
 
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[Histonet] patient release

[Houston, Ronald]

Would someone be willing to share a copy of their pathology slide patient 
release documentation, and if they have an inclusion acknowledging acceptance 
that they are responsible for any charges when the patient/family requests the 
second opinion?
Thanks
Ronnie Houston

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RE: [Histonet] Who is scanning thieir HE's slides for Path.

[Houston, Ronald]

Aperio

Ronnie Houston, MS HT(ASCP)QIHC
Anatomic Pathology Manager
ChildLab, a Division of Nationwide Children's Hospital
www.childlab.com

700 Children's Drive
Columbus, OH 43205
(P) 614-722-5450
(F) 614-722-2899
ronald.hous...@nationwidechildrens.org
www.NationwideChildrens.org

One person with passion is better than forty people merely interested.
~ E.M. Forster



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Stella Mireles
Sent: Tuesday, April 23, 2013 4:03 PM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] Who is scanning thieir HE's slides for Path.

We are gathering info. on the possibility of scanning our routine slides for 
our Path.
If you are doing this in your lab, would you mind sharing some info. on the 
eqiupment you are using?

Stella
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RE: [Histonet] RE: Pituitary gland

[Houston, Ronald]

back in the days when I worked exclusively in neuropathology in Glasgow, the 
tinctorial stains of choice for pituitary exams were PAS/OG and OFG (orange 
fuchsin green) methods.  I don't have the references to hand , although I 
believe the first was attributed to Tony Pearse, but can probably pull up the 
methods if needed


Ronnie Houston, MS HT(ASCP)QIHC
Anatomic Pathology Manager
ChildLab, a Division of Nationwide Children's Hospital
www.childlab.com

700 Children's Drive
Columbus, OH 43205
(P) 614-722-5450
(F) 614-722-2899
ronald.hous...@nationwidechildrens.org
www.NationwideChildrens.org

One person with passion is better than forty people merely interested.
~ E.M. Forster



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Geoff
Sent: Thursday, April 18, 2013 10:29 AM
To: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] RE: Pituitary gland

In our library all of these are still known as /Stain Technology/. The name 
change to /Biotechnic and Histochemistry/ happened much later.

Geoff


On 4/17/2013 9:14 PM, Tony Henwood (SCHN) wrote:
 Some references that might be useful:

 Paget, G. E.,  Eccleston, E. (1960). Simultaneous specific demonstration of 
 thyrotroph, gonadotroph and acidophil cells in the anterior hypophysis. 
 Biotechnic  Histochemistry, 35(3), 119-122.

 Phifer, R. F., Spicer, S. S.,  Hennigar, G. R. (1973). Histochemical 
 reactivity and staining properties of functionally defined cell types in the 
 human adenohypophysis. The American journal of pathology, 73(3), 569.

 Kerenyi, N. (1959). Congo red as a simple stain for beta cells of the 
 hypophysis. Biotechnic  Histochemistry, 34(6), 343-346.


 Elftman, H. (1959). Combined aldehyde-fuchsin and periodic acid-Schiff 
 staining of the pituitary. Biotechnic  Histochemistry, 34(2), 77-80.



 Read More: 
 http://informahealthcare.com/doi/abs/10.3109/10520295909114648

 Regards
 Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) 
 Laboratory Manager  Senior Scientist
 Tel: 612 9845 3306
 Fax: 612 9845 3318
 the children's hospital at westmead
 Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, 
 Westmead NSW 2145, AUSTRALIA

 -Original Message-
 From: histonet-boun...@lists.utsouthwestern.edu 
 [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of 
 Bustamante, Lin
 Sent: Thursday, 18 April 2013 1:45 AM
 To: histonet@lists.utsouthwestern.edu
 Subject: [Histonet] Pituitary gland

 Looking for any stain that shows acidophilic and basophilic cell from 
 pituitary gland.
 Thank you.
 Lin.


 Lin S. Bustamante, B.S., H.T.(ASCP)
 VIBS Histology Laboratory Supervisor
 College Of Veterinary Medicine
 Texas AM University
 College Station, Texas 77843-4458
 Phone: (979) 845-3177
 Fax: (979) 458-3499

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Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcaul...@umdnj.edu
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[Histonet] RE: Thioflavine S for Amyloid

[Houston, Ronald]

Jean,

obvious disadvantage is you need a fluorescence microscope, but then you need 
polarizing filters for Congo red (not as expensive though!)



I haven't used Thioflavine S but Thioflavine T. My understanding is that 
Thioflavine S, which is a chemically different dye, tends to fluoresce strongly 
but nonselectively, and can overstain the whole section. Either way, the 
staining is not light-fast!



Sections need to be kept in the dark until examined and usually within no more 
than a couple of days.



I used it a lot back home where it seemed to be preferred more than Congo red. 
It is a very simple stain to perform, and obviously can be screened quickly. 
Dissolving the dye in 0.1M HCl gives greater selectivity. The dye soluton has a 
shelf-life of a month or more if kept in the dark.



It is important to counterstain the sections with Mayer's hematoxylin as this 
quenches any nuclear fluorescence.



Elastic fibers, fibrinoid, mast cell granules and Paneth cell granules 
fluoresce with this method but they can be easily identified as such and ignored



The method of Burns et al gives probably the most consistent results: Burns J, 
Pennock CA, Stoward PJ. The specificity of staining of amyloid deposits with 
Thioflavine T. J Pathol Bacteriol 1967; 94: 337-344



Intersting comments  in Puchtler's paper: Puchtler H, Sweat Waldrop F, Meloan 
S. Application of thiazole dyes to amyloid under conditions of direct  cotton 
dyeing: correlation of histochemical and chemical data.  Histochemistry 1983; 
77: 431-445





Ronnie


Ronnie Houston, MS HT(ASCP)QIHC
Anatomic Pathology Manager
ChildLab, a Division of Nationwide Children's Hospital
www.childlab.com

700 Children's Drive
Columbus, OH 43205
(P) 614-722-5450
(F) 614-722-2899
ronald.hous...@nationwidechildrens.orgmailto:ronald.hous...@nationwidechildrens.org
www.NationwideChildrens.orghttp://www.nationwidechildrens.org/

One person with passion is better than forty people merely interested.
~ E.M. Forster



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mitchell Jean A
Sent: Friday, April 12, 2013 2:21 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Thioflavine S for Amyloid



Would appreciated some feedback/input from labs using Thioflavine S staining 
protocol for amyloid screening.  Any advantages/disadvantages to this procedure 
vs Congo Red?



Thanks much!!



Jean Mitchell, BS HT (ASCP)

University of Wisconsin Hospital  Clinics Neuromuscular Laboratory

600 Highland Avenue

Madison, WI  53792-5132







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[Histonet] Senior Histoetech vacancy

[Houston, Ronald]

We have a vacancy for a Senior Histotechnian in our progressive department. 
Experience in IHC a must. IF, ISH and /or EM desirable. Must be willing to 
participate in and lead part of formal Continuing Education program for all  
staff. Experience in QA development and Lean/Six Sigma projects a plus.

Please contact me for more details or apply on-line at 
http://www.nationwidechildrens.org/healthcare-career-opportunities

Thanks
Ronnie

Ronnie Houston, MS HT(ASCP)QIHC
Anatomic Pathology Manager
ChildLab, a Division of Nationwide Children's Hospital
www.childlab.com

700 Children's Drive
Columbus, OH 43205
(P) 614-722-5450
(F) 614-722-2899
ronald.hous...@nationwidechildrens.orgmailto:ronald.hous...@nationwidechildrens.org
www.NationwideChildrens.orghttp://www.nationwidechildrens.org/

One person with passion is better than forty people merely interested.
~ E.M. Forster

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[Histonet] RE: p57

[Houston, Ronald]

Jean,
we use Leica's clone Kip2 on placentas on our Bonds; 1:150

Ronnie

Ronnie Houston, MS HT(ASCP)QIHC
Anatomic Pathology Manager
ChildLab, a Division of Nationwide Children's Hospital
www.childlab.com

700 Children's Drive
Columbus, OH 43205
(P) 614-722-5450
(F) 614-722-2899
ronald.hous...@nationwidechildrens.orgmailto:ronald.hous...@nationwidechildrens.org
www.NationwideChildrens.orghttp://www.nationwidechildrens.org/

One person with passion is better than forty people merely interested.
~ E.M. Forster



From: ih...@googlegroups.com [mailto:ih...@googlegroups.com] On Behalf Of 
Taylor, Jean
Sent: Tuesday, March 19, 2013 12:19 PM
To: 'histonet@lists.utsouthwestern.edu'; 'ih...@googlegroups.com'
Subject: [IHCRG] p57

Hi everyone,

Would anyone be willing to share information about what clone/company/dilution 
you use for p57? We are looking to use this antibody on formalin fixed/paraffin 
embedded human placenta.

Thanks,
Jean Taylor, HT(ASCP)QIHC
Immuno Tech
Meriter Health Services
Madison, WI
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[Histonet] FISH baking and dewaxing

[Houston, Ronald]

Can someone please explain why paraffin-embedded slides for FISH need to be 
baked for so long and have extensive dewaxing in xylenes and subsequent alcohol 
pretreatment?
Our Molecular group bake for 1 hr at 65C, and then treat sections in 3 changes 
of xylene 15 min each, and 100% ethanol 15 minutes each x2 before air-drying. I 
have asked them why so long and get the standard response -- that's the way we 
always do it!

Thanks
Ronnie

Ronnie Houston, MS HT(ASCP)QIHC
Anatomic Pathology Manager
ChildLab, a Division of Nationwide Children's Hospital
www.childlab.com

700 Children's Drive
Columbus, OH 43205
(P) 614-722-5450
(F) 614-722-2899
ronald.hous...@nationwidechildrens.orgmailto:ronald.hous...@nationwidechildrens.org
www.NationwideChildrens.orghttp://www.nationwidechildrens.org/

One person with passion is better than forty people merely interested.
~ E.M. Forster

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[Histonet] Kaposi's Sarcoma

[Houston, Ronald]

Does anyone have a paraffin block of Kaposi's they could share?
Thanks

Ronnie Houston, MS HT(ASCP)QIHC
Anatomic Pathology Manager
ChildLab, a Division of Nationwide Children's Hospital
www.childlab.com

700 Children's Drive
Columbus, OH 43205
(P) 614-722-5450
(F) 614-722-2899
ronald.hous...@nationwidechildrens.orgmailto:ronald.hous...@nationwidechildrens.org
www.NationwideChildrens.orghttp://www.nationwidechildrens.org/

One person with passion is better than forty people merely interested.
~ E.M. Forster

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[Histonet] HT vacancy

[Houston, Ronald]

We have an immediate opening for a registered Histotechnician at Nationwide 
Childrens Hospital in Columbus, OH. Nationwide Childrens Hospital is the second 
largest free-standing pediatric facility in the nation. We offer a competitive 
salary and relocation allowance.
We are looking for an exemplary team player with a sound knowledge, both 
theoretical and practical, of microtomy, cryotomy, special stains, IHC, IF and 
ISH. Experience in electron microscopy a bonus, but full training will be 
provided in this and other specialties peculiar to pediatric pathology.

For more information and consideration, please contact me off-line or apply at 
www.nationwidechildrens.orghttp://www.nationwidechildrens.org
Thanks

Ronnie Houston, MS HT(ASCP)QIHC
Anatomic Pathology Manager
ChildLab, a Division of Nationwide Children's Hospital
www.childlab.comhttps://webmail.nationwidechildrens.org/owa/UrlBlockedError.aspx

700 Children's Drive
Columbus, OH 43205
(P) 614-722-5450
(F) 614-722-2899
ronald.hous...@nationwidechildrens.orgmailto:ronald.hous...@nationwidechildrens.org
www.NationwideChildrens.orghttp://www.nationwidechildrens.org/

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[Histonet] SOX-10

[Houston, Ronald]

Is anyone using SOX 10 in the diagnosis of soft tissue tumors? If so, can you 
please recommend a good reliable source for the antibody?

Thanks

Ronnie Houston, MS HT(ASCP)QIHC
Anatomic Pathology Manager
ChildLab, a Division of Nationwide Children's Hospital
www.childlab.com

700 Children's Drive
Columbus, OH 43205
(P) 614-722-5450
(F) 614-722-2899
ronald.hous...@nationwidechildrens.orgmailto:ronald.hous...@nationwidechildrens.org
www.NationwideChildrens.orghttp://www.nationwidechildrens.org/

One person with passion is better than forty people merely interested.
~ E.M. Forster



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RE: [Histonet] Can perform on IHC(P)

[Houston, Ronald]

don't understand why you would buy the antibody and then ask the question. The 
spec sheet indicates that the antibody has only been shown to work on WB, IP, 
IF and ELISA. There is no indication as to whether it will work on IHC.

Ronnie Houston

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of 
tahs...@brain.net.pk
Sent: Friday, September 07, 2012 1:03 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Can perform on IHC(P)

Dear All,
We have casein kinase sc-6479 santa cruz, can we perform on paraffin embedded 
section.
Thanks Muhammad tahseen



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[Histonet] Position - Snr Histotechnician

[Houston, Ronald]

We have a vacancy for a Senior Histotechnican. The Senior Histotechnician is 
responsible for performing a wide variety of histotechnological testing 
including, but not limited to, microtomy, cryotomy, complex special stains, IF, 
IHC, enzyme histochemistry and electron microscopy. The potential exists to 
become involved in research projects within the department. A working knowledge 
of Lean principles is useful. Theoretical knowledge as well as high quality 
practical ability is required. Candidates with expertise in 
Immunohistochemistry and/or Electron Microscopy preferred. Experience in 
pediatrics desirable. Registered HT(ASCP) or equivalent, with a minimum of 5 
years histopathology experience in a similar laboratory
Please apply on our web-site, and/or contact Sue Doud (614-355-41-5) or myself 
for more details
Thanks


Ronnie Houston, MS HT(ASCP)QIHC
Anatomic Pathology Manager
ChildLab, a Division of Nationwide Children's Hospital
www.childlab.com

700 Children's Drive
Columbus, OH 43205
(P) 614-722-5450
(F) 614-722-2899
ronald.hous...@nationwidechildrens.orgmailto:ronald.hous...@nationwidechildrens.org
www.NationwideChildrens.orghttp://www.nationwidechildrens.org/

One person with passion is better than forty people merely interested.
~ E.M. Forster



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RE: [Histonet] Luxol Fast Blue problem

[Houston, Ronald]

Luxol fast blue staining is dependent on the polarity of the solvent. Weak 
staining can occur if the solvent becomes too polar, i.e. if sections are taken 
down to water rather than 95% alcohol prior to putting in the staining 
solution. Also check the amount of water in the ethanol or methanol used to 
make up the LFB

Ronnie Houston, MS HT(ASCP)QIHC
Anatomic Pathology Manager
Nationwide Children's Hospital
Columbus OH 43205
(614) 722 5450


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tim Wheelock
Sent: Wednesday, June 27, 2012 11:10 AM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] Luxol Fast Blue problem

Hi again all:

Lately, I have had problems with my Luxol Fast Blue myelin stain not being 
nearly as dark as it should.
I am having my oven checked out  because  I have had problems with  it holding 
a  high enough temperature (at least 60C).
This seems to one factor in getting the stain to take.
But I suspect there may be other factors involved.
Has anyone had this sort of problem before? What did you find was the cause and 
how did you fix the problem?

Thanks for any advice you can provide.

Tim Wheelock
Harvard Brain Bank
McLean Hospital
Belmont, MA

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[Histonet] Controls for IF

[Houston, Ronald]

What are people using to control direct IF?

We use frozen tonsil for immunoglobulins and light chains, but what about 
fibrinogen, albumin, C1q, C3c and C4c?

Thanks

Ronnie Houston, MS HT(ASCP)QIHC
Anatomic Pathology Manager
ChildLab, a Division of Nationwide Children's Hospital
www.childlab.com

700 Children's Drive
Columbus, OH 43205
(P) 614-722-5450
(F) 614-722-2899
ronald.hous...@nationwidechildrens.orgmailto:ronald.hous...@nationwidechildrens.org
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[Histonet] CAP GEN.55500

[Houston, Ronald]

How are people tackling this General Competency question from CAP in Anatomic 
Pathology Labs, as in the strictest sense (in most cases) results are not being 
generated by the technical staff?

There is dispute between pathologists, techs and administrators about what 
would define a test system, and how specifically you would monitor the 
competency.

Would appreciate some ammunition

Thanks
Ronnie

Ronnie Houston, MS HT(ASCP)QIHC
Anatomic Pathology Manager
ChildLab, a Division of Nationwide Children's Hospital
www.childlab.com

700 Children's Drive
Columbus, OH 43205
(P) 614-722-5450
(F) 614-722-2899
ronald.hous...@nationwidechildrens.orgmailto:ronald.hous...@nationwidechildrens.org
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[Histonet] Interfacing CoPath with clients

[Houston, Ronald]

Does anyone have experience interfacing Sunquest CoPath with external clients 
so that they are able to access reports at will?

Ronnie Houston, MS HT(ASCP)QIHC
Anatomic Pathology Manager
ChildLab, a Division of Nationwide Children's Hospital
www.childlab.com

700 Children's Drive
Columbus, OH 43205
(P) 614-722-5450
(F) 614-722-2899
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[Histonet] charging for muscle biopsy

[Houston, Ronald]

What CPT codes would you use for a muscle biopsy sample that was dissected for 
frozen enzyme histochemistry, paraffin and EM?

Thanks
Ronnie


Ronnie Houston, MS HT(ASCP)QIHC
Anatomic Pathology Manager
ChildLab, a Division of Nationwide Children's Hospital
www.childlab.com

700 Children's Drive
Columbus, OH 43205
(P) 614-722-5450
(F) 614-722-2899
ronald.hous...@nationwidechildrens.orgmailto:ronald.hous...@nationwidechildrens.org
www.NationwideChildrens.orghttp://www.nationwidechildrens.org/

One person with passion is better than forty people merely interested.
~ E.M. Forster



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RE: [Histonet] Has anyone else noticed

[Houston, Ronald]

Joe,

you should never have water anywhere near Scotch. There's more than enough 
water used in its making (uisge beatha - water of life)



Ronnie Houston, MS HT(ASCP)QIHC

Anatomic Pathology Manager

Nationwide Children's Hospital

Columbus OH 43205

(614) 722 5450



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tony Henwood 
(SCHN)
Sent: Tuesday, December 20, 2011 5:10 PM
To: 'Joe Nocito'; 'Della Speranza, Vinnie'; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Has anyone else noticed



Remember we must validate, repeat, validate, repeate, vaallidatee, 
reeepeeaaat, valid.



OMG its blurry again



Regards

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory 
Manager  Senior Scientist

Tel: 612 9845 3306

Fax: 612 9845 3318

the children's hospital at westmead

Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead 
NSW 2145, AUSTRALIA



-Original Message-

From: Joe Nocito [mailto:jnoc...@satx.rr.com]

Sent: Tuesday, 20 December 2011 11:26 AM

To: Tony Henwood (SCHN); 'Della Speranza, Vinnie'; 
histonet@lists.utsouthwestern.edu

Subject: Re: [Histonet] Has anyone else noticed





I wonder if it works with Scotch. Hmmm, seems like an experiment brewing.

JTT



- Original Message -

From: Tony Henwood (SCHN) tony.henw...@health.nsw.gov.au

To: 'Della Speranza, Vinnie' del...@musc.edu; 
histonet@lists.utsouthwestern.edu

Sent: Monday, December 19, 2011 4:04 PM

Subject: RE: [Histonet] Has anyone else noticed





I keep trying to verify this scientific discovery but everything is blurry by 
the time I get to the bottom of the bottle, Oh sorry I need to look in the 
glass?



Regards

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory 
Manager  Senior Scientist

Tel: 612 9845 3306

Fax: 612 9845 3318

the children's hospital at westmead

Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead 
NSW 2145, AUSTRALIA



-Original Message-

From: histonet-boun...@lists.utsouthwestern.edu

[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Della Speranza, 
Vinnie

Sent: Tuesday, 20 December 2011 3:00 AM

To: histonet@lists.utsouthwestern.edu

Subject: FW: [Histonet] Has anyone else noticed









From: Pam Barker [mailto:rel...@earthlink.net]

Sent: Monday, December 19, 2011 10:57 AM

To: Della Speranza, Vinnie

Subject: RE: [Histonet] Has anyone else noticed



hahahahaha Vinnie!

A nice Cab is a great anesthetic, and your observation is a great reason to 
open a bottle!!  Who's with me?  Happy Holidays!!

-Original Message-

From: histonet-boun...@lists.utsouthwestern.edu

histonet-boun...@lists.utsouthwestern.edu

Sent: 12/19/2011 10:31 AM

To: histonet@lists.utsouthwestern.edu

Subject: [Histonet] Has anyone else noticed







Wondering if anyone else has noticed that rinsing a wine glass with water

will turn a nice cabernet sauvignon residue blue (reminiscient of

hematoxylin staining solution) ??





Yes, with so little positive in the news these days, I am definitely

anesthetizing myself this holiday season.



Vinnie Della Speranza, MS, HTL(ASCP)

Manager for Anatomic Pathology Services

Medical University of South Carolina

165 Ashley Avenue MSC 908

Charleston, SC 29425

tel. 843-792-6353

fax. 843-792-8974











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[Histonet] EM fixative composition

[Houston, Ronald]

Trying to get a sense of how people are making up their glutaradelhyde for EM 
on clinical specimens.

Is anyone buying it in premade, and if so from whom?
What buffer system are you using? phosphate or cacodylate (or something else)?
Are you checking the osmolality of the fixative before use?

Thanks

Ronnie Houston, MS HT(ASCP)QIHC
Anatomic Pathology Manager
ChildLab, a Division of Nationwide Children's Hospital
www.childlab.com

700 Children's Drive
Columbus, OH 43205
(P) 614-722-5450
(F) 614-722-2899
ronald.hous...@nationwidechildrens.orgmailto:ronald.hous...@nationwidechildrens.org
www.NationwideChildrens.orghttp://www.nationwidechildrens.org/

One person with passion is better than forty people merely interested.
~ E.M. Forster



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[Histonet] RE: Using methyl benzoate on a VIP 1000

[Houston, Ronald]

not familiar with its use nowadays, but I remember it being combined with 
celloidin (methyl benzoate celloidin) as part of a double-embedding protocol 
used for autopsy tissues. Personally I have not heard of it being used alone. 

Can still remember floating in the room where we had all our open processors 
with chloroform, methyl benzoate, alcohols, xylene, celloidin and ether in the 
atmosphere. Oh happy days - we were still high when we got to the pub after 
work!

Ronnie Houston, MS HT(ASCP)QIHC
Anatomic Pathology Manager
Nationwide Children's Hospital
Columbus OH 43205
(614) 722 5450


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kevin Seguin
Sent: Friday, December 02, 2011 2:40 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Using methyl benzoate on a VIP 1000

I have a customer who would like to use methyl benzoate on a VIP 1000.  Sakura 
says that it is not on the list of reagents so it is not recommended.  Can 
anyone shed some light on using methyl benzoate with a VIP 1000 or other tissue 
processors?  Will this work?  What are the problems?

Thanks!



Best regards,

Kevin Seguin
GMI
www.gmi-inc.comhttp://www.gmi-inc.com
kseg...@gmi-inc.commailto:kseg...@gmi-inc.com
800-745-2710



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[Histonet] fresh specimens after hours

[Houston, Ronald]

for facilities that are NOT staffed 24/7, how are fresh specimens handled after 
hours?

Up till now, OR staff have called the pathologist on-call to determine how best 
to handle the specimen, but that process has broken down on the OR side of 
things. The OR Director does not want 2 different ways of dealing with fresh 
specimens and they now want a process that will be the same 24/7.

As we will not be staffing the lab 24/7 on the odd chance a fresh specimen 
might come, I am interested in what other facilities may be doing.

Thanks
Ronnie


Ronnie Houston, MS HT(ASCP)QIHC
Anatomic Pathology Manager
ChildLab, a Division of Nationwide Children's Hospital
www.childlab.com

700 Children's Drive
Columbus, OH 43205
(P) 614-722-5450
(F) 614-722-2899
ronald.hous...@nationwidechildrens.orgmailto:ronald.hous...@nationwidechildrens.org
www.NationwideChildrens.orghttp://www.nationwidechildrens.org/

One person with passion is better than forty people merely interested.
~ E.M. Forster



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[Histonet] calmodulin

[Houston, Ronald]

Anyone had success getting Calmodulin up and running? I'm running in to some 
difficulties with an Abcam antibody one of our researchers gave me.

Thanks for any assistance
Ronnie


Ronnie Houston, MS HT(ASCP)QIHC
Anatomic Pathology Manager
ChildLab, a Division of Nationwide Children's Hospital
www.childlab.com

700 Children's Drive
Columbus, OH 43205
(P) 614-722-5450
(F) 614-722-2899
ronald.hous...@nationwidechildrens.orgmailto:ronald.hous...@nationwidechildrens.org
www.NationwideChildrens.orghttp://www.nationwidechildrens.org/

One person with passion is better than forty people merely interested.
~ E.M. Forster



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[Histonet] RE: IDH1 and IDH2 antibodies, MHC-Class I

[Houston, Ronald]

http://www.dianova.com/en/

Good luck...

Ronnie

Ronnie Houston, MS HT(ASCP)QIHC
Anatomic Pathology Manager
ChildLab, a Division of Nationwide Children's Hospital
www.childlab.com

700 Children's Drive
Columbus, OH 43205
(P) 614-722-5450
(F) 614-722-2899
ronald.hous...@nationwidechildrens.org
www.NationwideChildrens.org

One person with passion is better than forty people merely interested.
~ E.M. Forster



From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] on behalf of Martha Ward-Pathology 
[mw...@wakehealth.edu]
Sent: Monday, November 21, 2011 4:28 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] IDH1 and IDH2 antibodies, MHC-Class I

Hello all,

I have had a request from our neuropathologists to bring these antibodies in 
house but I am having some trouble finding vendors for them.   I would 
appreciate any information.

Thanks!

Martha Ward, MT (ASCP) QIHC
Manager, Molecular Diagnostics Lab
Dept. of Pathology
Wake Forest University Baptist Medical Center
Winston-Salem, NC 27157
336-716-2104

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RE: [Histonet] is there a way to recover ruined tissues

[Houston, Ronald]

Patsy
I have used a technique similar to the one in this article when I was fortunate 
to work on some mummified tissue, with surprising success, when I was back in 
Glasgow many, many moons ago

Mekota A-M, Vermehren MDetermination of optimal rehydration, fixation and 
staining methods for histological and immunohistochemical analysis of mummified 
soft tissues. Biotechnic  Histochemistry 2005, 80(1): 7-13

Ronnie

Ronnie Houston, MS HT(ASCP)QIHC
Anatomic Pathology Manager
Nationwide Children's Hospital
Columbus OH 43205
(614) 722 5450


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Shelly 
Christenson
Sent: Wednesday, November 16, 2011 10:06 AM
To: Patsy Ruegg; 'histo net'
Subject: RE: [Histonet] is there a way to recover ruined tissues

Patsy,
I have used this reprocessing schedule from Lee Luna's book Histopathologic 
Methods and Color Atlas of Specials Stains and Tissue Artifact. This procedure 
has worked for me on a couple of occasions, You may need to change the times a 
little since your tissue samples are so small. Luna's book has a lot of 
information that is very helpful, and would be a good reference book to have in 
the lab. Hope this helps you out, give it a try.

Tissue Softening Solution
The following solution is used for softening dried tissue specimens prior to 
reprocessing or processing

  Formol-Sodium Acetate (Stock) Solution
Concentrated formalin (37%) solution10 ml
Sodium acetate 2 gm
Tap water 90 ml

 Formol-Glycerol (Working) Solution
Formalin-sodium acetate (stock)  90 ml
Glycerol (glycerin)  10 ml
 
   Reprocessing Schedule
Melt the paraffin blocks down
Place the tissue in 3 changes of xylene for 1 hour each Place in 100% alcohol, 
2 changes for 1 hour each Place in 95% alcohol, 2 changes for 1 hour each Place 
under running tap water for 20 minutes Place in formol-glycerol working 
solution until tissue becomes soft (In most instances this will occur within 
5-8 hours: extended exposure to the formol-gycerol will not harm tissue 
specimens) Place the tissue in the process and proceed with routine Processing 
schedule. How ever it is not necessary for the tissue to go through the 
fixative stations on the processor


Best of Luck,
Shelly



 

Shelly Christenson HT (ASCP)

Veterinary Diagnostic Laboratory

Histopathology

L-216 Mosier Hall

Kansas State University

785/532-4464


From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] on behalf of Patsy Ruegg 
[pru...@ihctech.net]
Sent: Tuesday, November 15, 2011 2:26 PM
To: 'histo net'
Subject: [Histonet] is there a way to recover ruined tissues

My tissue processor was loaded and although the technician thought they started 
it, the next morning the tissues were found dry in the retort with the 
processor not started.  Another technician just started the machine so the 
tissues got processed as they would have been had it started the day before.  
The tissues look terrible, they shrank so much that the pieces started coming 
out of the cassettes even though they were plenty large enough in the first 
place.  The tissues were found to be very small and hard but were embedded, 
sectioned and stained anyway (I was not informed about this until I started 
questioning why the tissues looked so bad).



Of course the investigator is very upset that their tissues are ruined.
Does anyone know if there is any way to try and recovery these tissues?
They were mouse lungs and livers.  The livers are not as bad as the lungs, the 
lungs were inflated and they look compressed and unrecognizable as lung tissue. 
 If the investigator will ever speak to me again I am going to ask for the 
blocks back and try melting them down and perhaps try to rehydrate them somehow 
for reprocessing.



Regards,



Patsy



Patsy Ruegg, HT(ASCP)QIHC

IHCtech

12635 Montview Blvd. Ste.215

Aurora, CO 80045

720-859-4060

fax 720-859-4110

www.ihctech.net

www.ihcrg.org



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[Histonet] calmodulin

[Houston, Ronald]

Anyone had success getting Calmodulin up and running? I'm running in to some 
difficulties with an Abcam antibody one of our researchers gave me.

Thanks for any assistance
Ronnie


Ronnie Houston, MS HT(ASCP)QIHC
Anatomic Pathology Manager
ChildLab, a Division of Nationwide Children's Hospital
www.childlab.com

700 Children's Drive
Columbus, OH 43205
(P) 614-722-5450
(F) 614-722-2899
ronald.hous...@nationwidechildrens.orgmailto:ronald.hous...@nationwidechildrens.org
www.NationwideChildrens.orghttp://www.nationwidechildrens.org/

One person with passion is better than forty people merely interested.
~ E.M. Forster



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[Histonet] Rb gene protein antibody

[Houston, Ronald]

Can someone recommend the best clone for Retinoblastoma Gene Protein on 
formalin-fixed paraffin sections?

Thanks
Ronnie


Ronnie Houston, MS HT(ASCP)QIHC
Anatomic Pathology Manager
ChildLab, a Division of Nationwide Children's Hospital
www.childlab.com

700 Children's Drive
Columbus, OH 43205
(P) 614-722-5450
(F) 614-722-2899
ronald.hous...@nationwidechildrens.orgmailto:ronald.hous...@nationwidechildrens.org
www.NationwideChildrens.orghttp://www.nationwidechildrens.org/

One person with passion is better than forty people merely interested.
~ E.M. Forster



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RE: [Histonet] Re: Celestin blue B (was Help)

[Houston, Ronald]

I believe Celestine Blue was used much more frequently in the UK in the 70s and 
80s, preferred to an iron hematoxylin, in many trichrome stains including 
Lendrum's MSB, as Peggy mentions; the nuclear staining withstanding further 
differentiation during the subsequent staining from either picric or 
phosphotungstic/phosphomolybdic acids.
It is a difficult stain to prepare as it has to be boiled with glycerin in it 
and requires glass beads being added to prevent it foaming up and going all 
over the bench (don't have the exact recipe to hand, but do remember having to 
frequently clean up the mess during my training!) We used it extensively, and I 
do not remember it having a particularly short shelf-life - but it did have to 
be filtered regularly.


Ronnie Houston, MS HT(ASCP)QIHC
Anatomic Pathology Manager
Nationwide Children's Hospital
Columbus OH 43205
(614) 722 5450

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bob Richmond
Sent: Sunday, November 06, 2011 3:42 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Re: Celestin blue B (was Help)

Corrie Vernick writes:

I am currently a histology student at Keiser University. I am doing a 
project for my routine staining class about Celestine Blue. I've been 
able to find information on why it was created, the chemical make up, 
and some of it's uses including the trichrome stain. I am having 
trouble finding images of slides stained with Celestine Blue. Any 
additional information about the uses would be helpful as well! Thank 
you, Corrinne Vernick, Keiser University FL U.S.A.

I don't have access to my library this week, but you can get a good bit 
information by Googling celestin blue B. This dye was often used as a sort of 
backup or substitute for hematoxylin, particularly in the old outmoded Pearse 
stain for pituitary cells. R.D. Lillie as I remember didn't think much of the 
dye, and I don't think this dye is a very good topic for a study such as the 
one you describe.

Bob Richmond
Samurai Pathologist
Knoxville TN

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RE: [Histonet] Specimen Tracking for courier

[Houston, Ronald]

Our lab as a whole uses the Gajema Specimen Transport And Tracking System for 
all courier deliveries


Ronnie Houston, MS HT(ASCP)QIHC
Anatomic Pathology Manager
Nationwide Children's Hospital
Columbus OH 43205
(614) 722 5450

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cynthia Pyse
Sent: Thursday, November 03, 2011 11:13 AM
To: 'Bull, Jennifer L.'; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Specimen Tracking for courier

I would be interested in everyone responses.

Cindy

Cindy Pyse, CLT, HT (ASCP)
Laboratory Manger
X-Cell Laboratories
e-mail cp...@x-celllab.com



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bull, Jennifer 
L.
Sent: Thursday, November 03, 2011 10:43 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Specimen Tracking for courier



I'm curious what methods other labs are using to track specimens that couriers 
pick up from clinic locations to ensure safe delivery to the lab.
We currently utilize a logbook at the clinic site that the courier signs when 
they take the specimen but I have heard there are barcoded systems available 
out there as well. What works? What doesn't?  Appreciate your feedback!

Jenny


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[Histonet] TGF beta

[Houston, Ronald]

Having some trouble getting any staining with AbCam's TGF beta antibody? Any 
recommendations



Thanks

Ronnie Houston


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[Histonet] RE: staining for endothelial glycocalyx

[Houston, Ronald]

sounds like a job for Lectin Histochemistry

Ronnie Houston
Anatomic Pathology Manager
Nationwide Children's Hospital
Columbus OH 43205
(614) 722 5450
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Featherstone, 
Annette
Sent: Tuesday, October 11, 2011 2:21 PM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] staining for endothelial glycocalyx

Does anyone have a staining protocol that will stain sugar structures on the 
surface of the endothelial cells?? Commonly structures are visable using SEM 
but I would like to know if it is possible to see it in a paraffin section.

Annette
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RE: Re: [Histonet] Von Kossa stain kit

[Houston, Ronald]

I've used Rowley Biochemical for years:  http://rowleybio.com/


Ronnie Houston
Anatomic Pathology Manager
Nationwide Children's Hospital
Columbus OH 43205
(614) 722 5450

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sherwood, 
Margaret
Sent: Tuesday, October 04, 2011 11:03 AM
To: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Von Kossa stain kit

Thought of another question!  What other vendors do people order special stain 
kits?  We have been using Poly Scientific, which is very good, but costly.  

Thanks!
Peggy 

Peggy Sherwood
Lab Associate, Photopathology
Wellman Center for Photomedicine (EDR 214) Massachusetts General Hospital
50 Blossom Street
Boston, MA 02114-2696
617-724-4839 (voice mail)
617-726-6983 (lab)
617-726-1206 (fax)
msherw...@partners.org



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RE: [Histonet] decal question

[Houston, Ronald]

Alcoholic formalin or 70% ethanol as, quoting Gayle Callis, alcohol can slow 
or prevent decalcification

Ronnie Houston
Anatomic Pathology Manager
Nationwide Children's Hospital
Columbus OH 43205
(614) 722 5450
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg
Sent: Monday, October 03, 2011 12:32 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] decal question

Hi Everyone,

 

I have a new student taking course work at UND and using my lab for her 
practical clinical site.  She took a test on decal today and there was a 
question we didn't know the answer to.

 

What fixative should not be used for acid decalcification?  Would it be osmium 
tetroxide?

 

Patsy Ruegg, HT(ASCP)QIHC
IHCtech, LLC
Fitzsimmons BioScience Park
12635 Montview Blvd. Suite 215
Aurora, CO 80010
P-720-859-4060
F-720-859-4110
wk email  mailto:pru...@ihctech.net pru...@ihctech.net web site  
http://www.ihctech.net www.ihctech.net

 


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privileged  confidential within the meaning of applicable law. Accordingly any 
dissemination, distribution, copying, or other use of this message, or any of 
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intended recipient please contact the sender and dispose of this e-mail as soon 
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[Histonet] RE: Nuclear Fast Red Substitute?

[Houston, Ronald]

1% aq neutral red (CI 50040)

Ronnie Houston
Anatomic Pathology Manager
Nationwide Children's Hospital
Columbus OH 43205
(614) 722 5450
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara
Sent: Thursday, September 08, 2011 9:09 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Nuclear Fast Red Substitute?

What can I use as a substitute for NFR for Prussian Blue iron stain, please?

 

Sally Breeden, HT(ASCP)

New Mexico Department of Agriculture

Veterinary Diagnostic Services

1101 Camino de Salud NE

Albuquerque, NM  87102

505-383-9278 (Histology Lab)

 

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RE: [Histonet] MSDS binders

[Houston, Ronald]

There must be some way for the Fire Department to be able to access MSDS. As 
Victor rightly points out, if your computer system is down, that option no 
longer exists. It is not only prudent, it is mandatory for the Fire Service to 
be able to look up any chemical within your facility.

If you cannot satisfy your local fire daprtment, they will close you down 
quicker than CAP or CLIA ever could.


Ronnie Houston
Anatomic Pathology Manager
Nationwide Children's Hospital
Columbus OH 43205
(614) 722 5450

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Victor Tobias
Sent: Thursday, August 25, 2011 3:16 PM
To: William
Cc: Histonet@Lists. Edu
Subject: Re: [Histonet] MSDS binders

  In practicality you would turn to your online version first. What happens if 
the computer system/network is down. Seems like it would be prudent to have a 
hard copy available.

Victor

Victor Tobias HT(ASCP)
Clinical Applications Analyst
University of Washington Medical Center
Dept of Pathology Room BB220
1959 NE Pacific
Seattle, WA 98195
vic...@pathology.washington.edu
206-744-2735
206-744-8240 Fax
=
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On 8/25/2011 12:08 PM, William wrote:
 Not certain about CLIA, but CAP only requires immediate available access to 
 the MSDS's by any personal using chemicals. I interpret that as digital 
 copies ok, as long as everyone has access. I have passed three inspections 
 with digital MSDS only.

 Will Chappell

 Sent from my iPhone

 On Aug 25, 2011, at 11:58 AM, Jill Coxjco...@yahoo.com  wrote:

 Hello Histonetters,

 Does anyone know if there is a rule as to MSDS binders having to be in 
 yellow and black bold lettering? Do we even still need to have hard copy if 
 we have access to msds.com on desk top? I am in Ca and will be CLIA 
 licensed.. Thank you in advance!!

 Jill Cox, HT ASCP
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[Histonet] RE: Nile Red stain on Froz. muscle bx's

[Houston, Ronald]

J Histochem Cytochem. 1987 May;35(5):619-21.
Application of nile blue and nile red, two fluorescent probes, for detection of 
lipid droplets in human skeletal muscle.
Bonilla E, Prelle A.
Abstract
Using frozen sections from human muscle biopsies, we assessed the value of Nile 
blue and Nile red, two fluorescent probes, as stains for lipid droplets in 
normal and pathological skeletal muscle fibers. In normal muscle, lipid storage 
disorders, and mitochondrial myopathies, Nile blue stained the lipid droplets 
as yellow-gold fluorescent structures. The lipid droplets were also seen as 
yellow-gold fluorescent structures in Nile red-stained sections, but the 
outstanding feature in these preparations was the staining of the membrane 
network of the muscle fibers and membrane proliferations in pathological muscle 
as red-orange fluorescent structures. These results suggest that both Nile blue 
and Nile red stains are useful for visualization of lipid droplets and membrane 
proliferations in pathological muscle biopsies.

PMID:3559182[PubMed - indexed for MEDLINE]



Ronnie Houston
Anatomic Pathology Manager
Nationwide Children's Hospital
Columbus OH 43205
(614) 722 5450
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sharon Allen
Sent: Tuesday, August 23, 2011 10:11 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Nile Red stain on Froz. muscle bx's

Hi,
Does anyone have a method for staining frozen skeletal muscle bx's with Nile 
Red /or Nile Blue for the detection of lipid droplets.
Our Neuropathologist saw the stain at a conference  would like us to try it. 
Thanks

Sharon Allen
Senior Technologist
Neuropathology Lab MS435U
Health Sciences Centre
825 Sherbrook St.
Winnipeg, Manitoba R3A 1R9
Ph# 787-4615


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[Histonet] Eosinophil Cationic Protein

[Houston, Ronald]

Does anyone know of a source of ECP for use  on human paraffin embedded 
material? Pharmacia used to carry it but since they were bought out it is only 
availble as part of an ELISA kit?
Thanks

Ronnie Houston, MS HT(ASCP)QIHC
Anatomic Pathology Manager
ChildLab, a Division of Nationwide Children's Hospital
www.childlab.com

700 Children's Drive
Columbus, OH 43205
(P) 614-722-5450
(F) 614-722-2899
ronald.hous...@nationwidechildrens.orgmailto:ronald.hous...@nationwidechildrens.org
www.NationwideChildrens.orghttp://www.NationwideChildrens.org

One person with passion is better than forty people merely interested.
~ E.M. Forster


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[Histonet] TFE-3

[Houston, Ronald]

Is anyone using TFE-3? If so I would appreciate which clone is being used most.
Thanks

Ronnie Houston, MS HT(ASCP)QIHC
Anatomic Pathology Manager
ChildLab, a Division of Nationwide Children's Hospital
www.childlab.com

700 Children's Drive
Columbus, OH 43205
(P) 614-722-5450
(F) 614-722-2899
ronald.hous...@nationwidechildrens.orgmailto:ronald.hous...@nationwidechildrens.org
www.NationwideChildrens.orghttp://www.NationwideChildrens.org

One person with passion is better than forty people merely interested.
~ E.M. Forster


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[Histonet] C4d nuclear staining?

[Houston, Ronald]

Has anyone ever noticed any nuclear staining with C4d in a renal biopsy? The 
control stains great, but the patient's tissue exhibits some nuclear staining 
in what look to be inflammatory cells. There has been a report of granular and 
some nuclear staining in lung transplants on structures beyond the vasculature 
but we haven't seen anything like this in a case of renal rejection.

Ronnie Houston, MS HT(ASCP)QIHC
Anatomic Pathology Manager
ChildLab, a Division of Nationwide Children's Hospital
www.childlab.com

700 Children's Drive
Columbus, OH 43205
(P) 614-722-5450
(F) 614-722-2899
ronald.hous...@nationwidechildrens.orgmailto:ronald.hous...@nationwidechildrens.org
www.NationwideChildrens.orghttp://www.NationwideChildrens.org

One person with passion is better than forty people merely interested.
~ E.M. Forster


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[Histonet] RE: Cresyl violet stain on 50 um mouse brain sections

[Houston, Ronald]

Susan,
If you are talking about frozen sections of fixed tissue, they do not adhere 
well to slides (even charged slides) no matter how thick or thin. I'm not sure 
why you are treating the sections with alcohol chloroform either. That is 
pretty harsh treatment and totally unnecessary for Nissl staining. 

Why not stain the sections free-floating and then mount them from xylene?

Ronnie Houston
Anatomic Pathology Manager
Nationwide Children's Hospital
Columbus OH 43205
(614) 722 5450

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Truscott, Tom
Sent: Friday, July 29, 2011 10:22 AM
To: Michael, Susan; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Cresyl violet stain on 50 um mouse brain sections

Hi Susan, I would suggest cutting lots thinner sections. The plus slides seem 
to have trouble holding on to very thick sections. Another possibility is too 
much glacial acetic acid in your working solution of cresyl-too much acid can 
loosen the bonds on the plus slides. Tom T

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Michael, Susan
Sent: Friday, July 29, 2011 6:12 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Cresyl violet stain on 50 um mouse brain sections

I am having trouble keeping my sections on the slides when I stain with cresyl 
violet.  These are fixed frozen sections, 50 um, dried overnight on plus 
slides, overnight in 1 to 1 alcohol/chloroform, then rehydrated through 100, 95 
ETOHs then to water.  Into the cresyl violet solution, then 100% to 95% ETOH.  
Is it the water?  Is it the slides?  Any suggestions?

Susan
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[Histonet] Cat Scratch control

[Houston, Ronald]

Does anyone have an extra Cat Scratch control block to spare? We currently buy 
control sections from Newcomer, but I'm not convinced they are that good.
Thanks

Ronnie Houston, MS HT(ASCP)QIHC
Anatomic Pathology Manager
ChildLab, a Division of Nationwide Children's Hospital
www.childlab.com

700 Children's Drive
Columbus, OH 43205
(P) 614-722-5450
(F) 614-722-2899
ronald.hous...@nationwidechildrens.orgmailto:ronald.hous...@nationwidechildrens.org
www.NationwideChildrens.orghttp://www.NationwideChildrens.org

One person with passion is better than forty people merely interested.
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[Histonet] RE: Re: Histobath revised

[Houston, Ronald]

I second Terri's comments

Ronnie Houston
Anatomic Pathology Manager
Nationwide Children's Hospital
Columbus OH 43205
(614) 722 5450

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Johnson, Teri
Sent: Monday, July 18, 2011 9:44 AM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] Re: Histobath revised

Dr. Richmond recommended using non-flammable and non-explosive Novec (tm) 
Engineered Fluid HFE-7100. We are currently using this for our freezing and it 
works very well. The main thing we have noticed is the blocks float in it and 
do not sink as they would with other solvents.

It is a 3M product, and my contact there last year was ebin...@mmm.com (Erin 
Binder).

Teri Johnson, HT(ASCP)QIHC
Head, Histology and Electron Microscopy
Stowers Institute for Medical Research
Kansas City, MO


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[Histonet] RE: ...maybe a little more specific

[Houston, Ronald]

Sarah, 
if you don't want to go to the hassle of working up antibodies on xenografts, 
you could use Ulex Europaeus lectin histochemistry which should work on any 
species - great for capillaries

Ronnie Houston
Anatomic Pathology Manager
Nationwide Children's Hospital
Columbus OH 43205
(614) 722 5450
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of 
sgoe...@mirnarx.com
Sent: Friday, July 01, 2011 2:03 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] ...maybe a little more specific

So am getting lots of responses for IHC stains (which is ok), but I am
doing these stains on mouse xenografts and the background is getting in
my way a lot.  Was just trying to find a simple special that would do
the trick =)

If IHC is the only way, so be it...was just hoping...

 

What about Verhoeff???

 

Sarah Goebel-Dysart, BA, HT(ASCP)

Histotechnologist

Mirna Therapeutics

2150 Woodward Street

Suite 100

Austin, Texas  78744

(512)901-0900 ext. 6912

 

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[Histonet] Educational Symposium in Columbus, OH

[Houston, Ronald]

For those of you interested in keeping up to date, please note there is an 
all-day Educational Symposium in Columbus, OH, on Thursday, July 14, 2011.

Keynote Addresses in the morning:
Standardization and Automation in Anatomic Pathology: How to meet the future 
needs for quality clinical care in a cost effective era
Syed K. Mohsin, MD
Head of Breast Pathology  Medical Director, IHC Lab, Riverside Methodist/Grant 
Hospitals, Columbus, Ohio

When is a Diagnosis not a Diagnosis? When it belongs to the Wrong Patient!
Doyle Carney, Manager Workflow Solutions - Biosystems Division, Leica 
Microsystems, Buffalo Grove, IL

Breakout sessions after lunch.

For more information, please contact your Leica rep, or I can forward program 
if interested

Thanks

Ronnie Houston, MS HT(ASCP)QIHC
Anatomic Pathology Manager

ChildLab, a Division of Nationwide Children's Hospital

www.childlab.com


700 Children's Drive
Columbus, OH 43205
(P) 614-722-5450
(F) 614-722-2899
ronald.hous...@nationwidechildrens.orgmailto:ronald.hous...@nationwidechildrens.org
www.NationwideChildrens.orghttp://www.NationwideChildrens.org

One person with passion is better than forty people merely interested.
~ E.M. Forster



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RE: [Histonet] Leica Bond for IHCs

[Houston, Ronald]

greatest thing since sliced bread!


From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] on behalf of Sheila Adey 
[sa...@hotmail.ca]
Sent: Saturday, June 25, 2011 12:00 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Leica Bond for IHCs

Hello netters:
Looking for opinions on the Leica Bond immuno stainer please.
Thanks.
Sheila
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[Histonet] RE: Ventana's HER2 Dual ISH DNA Probe Cocktail

[Houston, Ronald]

If you modify any part of the test protocol from the FDA approved methodology 
in any way, then it is no longer an FDA approved test.

Does that mean you cannot use it on your system? Absolutely not; you would have 
to validate it as you would any other antibody. 

However, you do need to be cognisant of the fact that if the FDA approved test 
is dependent on a patient going on a clinical trial or FDA approved treatment 
for a particular disease, your patient may not be accepted for that treatment 
regimen.

Ronnie Houston
Anatomic Pathology Manager
Nationwide Children's Hospital
Columbus OH 43205
(614) 722 5450

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Walter Benton
Sent: Monday, June 20, 2011 1:37 PM
To: Bell, Lynne; Histonet (histonet@lists.utsouthwestern.edu)
Subject: [Histonet] RE: Ventana's HER2 Dual ISH DNA Probe Cocktail

In the past the FDA approval has been tied to running the antibody on the 
specific vendors' instrumentation using a predetermined protocol. This was true 
for Dako's EGFR that was FDA approved.

Ventana antibodies can be dispensed out of their vials and used on any 
instrumentation, however, you may have an IVD antibody at that point.

Definitely check around and see what others have to say.

Walter Benton HT(ASCP)QIHC
Histology Supervisor
Chesapeake Urology Associates
806 Landmark Drive, Suite 126
(All Deliveries to Suite 127)
Glen Burnie, MD 21061
443-471-5850 (Direct)
410-768-5961 (Lab)
410-768-5965 (Fax)
wben...@cua.md

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bell, Lynne 
[lynne.b...@cvmc.org]
Sent: Monday, June 20, 2011 1:28 PM
To: Histonet (histonet@lists.utsouthwestern.edu)
Subject: [Histonet] Ventana's HER2 Dual ISH DNA Probe Cocktail

Ventana just received FDA approval for the first fully automated diagnostic 
assay for HER2 gene status determination in breast cancer patients.

I do not have a Ventana immunostainer and from what I have been told, it is a 
closed system and this new probe cocktail could not be used on another stainer, 
such as the Dako.

Is anyone out there in HistoLand using this new cocktail and am I correct that 
it would not work using the Dako Autostainer.

A Ventana rep may contact me with this information also.

Lynne Bell, HT (ASCP)
Histology Team Leader
Central Vermont Medical Center
Barre, Vermont  05641
802-371-4923


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[Histonet] Ki-67/PHH3 double staining

[Houston, Ronald]

Has anyone had success double staining these two antibodies for IHC? Ki-67 
tends to mask most of the PHH3 staining irrespective of which antibody we use 
first. Individually, both antibodies have clean and crisp localization. I have 
seen pictures in articles and on-line of results but am far from attaining 
anything like these.
We are using the Refine DAB and AP Red kits from Leica on the Bonds. Ki-67 is 
from Leica and PHH3 from Cell Marque

Any help or suggestions welcome
Thanks
Ronnie

Ronnie Houston, MS HT(ASCP)QIHC
Anatomic Pathology Manager

ChildLab, a Division of Nationwide Children's Hospital

www.childlab.com


700 Children's Drive
Columbus, OH 43205
(P) 614-722-5450
(F) 614-722-2899
ronald.hous...@nationwidechildrens.orgmailto:ronald.hous...@nationwidechildrens.org
www.NationwideChildrens.orghttp://www.NationwideChildrens.org

One person with passion is better than forty people merely interested.
~ E.M. Forster



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RE: [Histonet] Light + IF microscope

[Houston, Ronald]

APO stands for apochromatic, indicating an objective with the highest degree of 
correction for spherical and chromatic aberrations

Ronnie Houston
Anatomic Pathology Manager
Nationwide Children's Hospital
Columbus OH 43205
(614) 722 5450
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Emily Sours
Sent: Monday, June 13, 2011 3:46 PM
To: Bader Siddiki; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Light + IF microscope

Nikon is expensive, but they are very friendly and will fix little things
for you for free (not that they'll tell you that upfront).  Our Nikon
representative is always coming by if we have even the slightest problem and
is willing to figure out weird problems that come up.  Also, they always
check the alignment on our FL bulb because I really hate doing it and I can
never get it right (though it seems so damn easy when the guy is showing me
how to do it!).
We have a Nikon E600 that is decades old and still in perfect condition.
This scope is actually featured on the microscope museum page Nikon runs, I
was very surprised to see it there!
You should try talking to the representative to see if you can get some sort
of deal.  They are very friendly and very willing to work with you.  I would
tell you to talk to Adam Henry but he works for Pennsylvania only.  He's
excellent!
You might be able to get something used from them, you never know.
Personally, I'd pay for something new, no matter how expensive (of course,
who isn't working under a grant right now? me! so I shouldn't really talk
about spending loads of money!).  Nikon is a good brand and definitely worth
the cost.
Also, pay for the APO objectives, it's worth it.  You can tell the
difference.  I have no idea what APO stands for, but they work better than
regular ones.

Emily

A great book should leave you with many experiences, and slightly exhausted.
You should live several lives while reading it.
-William Styron



On Mon, Jun 13, 2011 at 3:10 PM, Bader Siddiki bade...@gmail.com wrote:

 Hello Histonetters
 I need your professional advice.
 We are a small IHC and IF company looking for a good microscope.
 We have investigated in Nikon;s they have 50i and 55i microscope that one
 can use for IHC and IF with proper attachment, but pretty steep price.
 Do you have any recommendation for a good quality microscope w/ IF
 attachments.
 May be one of you have a microscope like this and want to sell at a
 reasonable price.
 Your expert advice and recommendation will be appreciated.
 Thank you.
 Bader


 Bader B Siddiki, PhD
 Executive director,
 Research and development
 ImmunoBioScience Corp. (IBSC)
 Phone: + 1 425 367 4601
 Fax: + 1 425 367 4817
 cell (mobile) phone: + 1 425 314 0199
 e-mail address: bade...@gmail.com
 Web site: www.ImmunoBioScience.Com
 Marketing: phone: + 1 650 343 IBSC (4272)
E-mail: anitai...@aol.com
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[Histonet] PA traveler needed

[Houston, Ronald]

Would any recruiter who has a traveling PA available for 8-10 weeks coverage 
starting August please contact me off-line?
Thanks

Ronnie Houston, MS HT(ASCP)QIHC
Anatomic Pathology Manager

ChildLab, a Division of Nationwide Children's Hospital

www.childlab.com


700 Children's Drive
Columbus, OH 43205
(P) 614-722-5450
(F) 614-722-2899
ronald.hous...@nationwidechildrens.orgmailto:ronald.hous...@nationwidechildrens.org
www.NationwideChildrens.orghttp://www.NationwideChildrens.org

One person with passion is better than forty people merely interested.
~ E.M. Forster



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[Histonet] RE: off topic for the guys

[Houston, Ronald]

believe it is Solvent Red 164 (Oil Red B) that is used to color ATF

Ronnie Houston

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Della Speranza, Vinnie 
[del...@musc.edu]
Sent: Friday, May 27, 2011 1:12 PM
To: Grantham, Andrea L - (algranth); HISTONET
Subject: [Histonet] RE: off topic for the guys

OK, I saw Andrea's post and after all , it is Friday.
I saw a recent episode of CSI-New York that had a cochineal beetle as part of 
the crime scene evidence about a sniper. Anyway, it got me wondering, anyone 
know if automatic transmission fluid is colored with cochineal (carmine) ?? (I 
had to make this guy related and don't know any girls working on their 
transmissions)

Vinnie Della Speranza
Manager for Anatomic Pathology Services
165 Ashley Avenue Suite 309
Charleston, SC 29425
tel. 843-792-6353
fax. 843-792-8974


  Learn more about Histotechnology Professionals Day at
www.nsh.orghttp://www.nsh.org/


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Grantham, 
Andrea L - (algranth)
Sent: Thursday, May 26, 2011 3:29 PM
To: HISTONET
Subject: [Histonet] off topic/on topic

This is mostly for the girls - I dropped into the Ulta store yesterday to pick 
up a few things and the sales person wanted me to try this somewhat new product 
by Benefit. It was a lip and cheek tint - came in a little bottle - looked like 
mucicarmine working solution. I looked at the box that it came in and sure 
enough, one of the first ingredients was CARMINE! So I had to relate a few of 
the stories about beetles and Carmine and pirates that I learned from reading 
A Perfect Red recommended by Dick Dapson...wonder if I bored her? Maybe she 
won't bother me the next time I'm rushing through the store trying to get my 
stuff and get out of there.
Andi

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[Histonet] correct CPT code for anal smear

[Houston, Ronald]

What would be the correct CPT code for an anal smear collected in a SurePath 
vial (thin layer technology) for cytological evaluation and subsequent HPV 
testing?
Thanks

Ronnie Houston, MS HT(ASCP)QIHC
Anatomic Pathology Manager

ChildLab, a Division of Nationwide Children's Hospital

www.childlab.com


700 Children's Drive
Columbus, OH 43205
(P) 614-722-5450
(F) 614-722-2899
ronald.hous...@nationwidechildrens.orgmailto:ronald.hous...@nationwidechildrens.org
www.NationwideChildrens.orghttp://www.NationwideChildrens.org

One person with passion is better than forty people merely interested.
~ E.M. Forster



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[Histonet] Microprobe

[Houston, Ronald]

Looking for a new or used Fisher Microprobe. Please contact me off-line if you 
can help

Ronnie Houston, MS HT(ASCP)QIHC
Anatomic Pathology Manager

ChildLab, a Division of Nationwide Children's Hospital

www.childlab.com


700 Children's Drive
Columbus, OH 43205
(P) 614-722-5450
(F) 614-722-2899
ronald.hous...@nationwidechildrens.orgmailto:ronald.hous...@nationwidechildrens.org
www.NationwideChildrens.orghttp://www.NationwideChildrens.org




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[Histonet] IHC menu

[Houston, Ronald]

Would anyone be willing to share their IHC menu with us? I want to see how we 
compare with other top-class facilities. Obviously I am more interested in 
pediatric facilities, but we are constantly evaluating antibodies for research 
studies, and what may have applications in pediatric pathology from the adult 
world.

I'd be happy to send our menu on return
Thanks

Ronnie Houston, MS HT(ASCP)QIHC
Anatomic Pathology Manager

ChildLab, a Division of Nationwide Children's Hospital

www.childlab.com


700 Children's Drive
Columbus, OH 43205
(P) 614-722-5450
(F) 614-722-2899
ronald.hous...@nationwidechildrens.orgmailto:ronald.hous...@nationwidechildrens.org
www.NationwideChildrens.orghttp://www.NationwideChildrens.org

One person with passion is better than forty people merely interested.
~ E.M. Forster



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[Histonet] Fisher Microprobe

[Houston, Ronald]

If anyone has a used Fisher Microprobe system that is sitting around gathering 
dust, could you please contact me off-line?

I am in desperate need for an intra-op study we are proposing

Thanks
Ronnie

Ronnie Houston, MS HT(ASCP)QIHC
Anatomic Pathology Manager

ChildLab, a Division of Nationwide Children's Hospital

www.childlab.com


700 Children's Drive
Columbus, OH 43205
(P) 614-722-5450
(F) 614-722-2899
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RE: [Histonet] Tissue Processors

[Houston, Ronald]

I would probably have gone along with you on this Rene but since we demo'ed, 
and subsequently purchased, the Peloris my opinion has changed

Ronnie Houston
Anatomic Pathology Manager
Nationwide Children's Hospital
Columbus OH 43205
(614) 722 5450
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Friday, February 18, 2011 8:33 AM
To: Histonet; Joe Nocito
Subject: Re: [Histonet] Tissue Processors

Sahura is still the best buy (for me).
René J.

--- On Thu, 2/17/11, Joe Nocito jnoc...@satx.rr.com wrote:


From: Joe Nocito jnoc...@satx.rr.com
Subject: [Histonet] Tissue Processors
To: Histonet histonet@lists.utsouthwestern.edu
Date: Thursday, February 17, 2011, 8:55 PM


Greetings all,
if you had to purchase new tissue processors, which one would you choose? 
Microwave technology is out of the question. Are Sakura's still a good buy? 
We've tried the Leica Peloris and the Shandon Pathcenters. Thanks for your help

Joe
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[Histonet] RE: Azure B as a counter-stain for melanin

[Houston, Ronald]

Naira,

we use a standard working Giemsa solution that is good for 1 month, stored at 
4C. We automate the procedure on our Bonds: 3min Giemsa and 1 min Hematoxylin.


Ronnie Houston, MS HT(ASCP)QIHC
Anatomic Pathology Manager

ChildLab, a Division of Nationwide Children's Hospital

www.childlab.com


700 Children's Drive
Columbus, OH 43205
(P) 614-722-5450
(F) 614-722-2899
ronald.hous...@nationwidechildrens.orgmailto:ronald.hous...@nationwidechildrens.org

www.NationwideChildrens.orghttp://www.NationwideChildrens.org



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Margaryan, Naira
Sent: Friday, February 11, 2011 11:22 AM
To: histonet@lists.utsouthwestern.edu; histonet-requ...@lists.utsouthwestern.edu
Subject: [Histonet] Azure B as a counter-stain for melanin
Importance: High



Dear Histology Professionals,



I am using the Azure B as a counter-stain for melanin preparing solution by the 
protocol below. My questions are:

1. Is this solution should be fresh prepared every day?

2. How long I can store and use this solution?

3. Where this solution should be stored in the room or in the refrigerator?



My protocol is:

Counterstain Method

  The use of Azure B as a counter-stain will stain melanin a blue green 
color.

Azure B working reagent:

4mL of a 25mg/mL aqueous solution of Azure B

3.4mL of 0.1M acetic acid

600uL of 0.1M sodium acetate

27mL of DI water

5mL acetone



Incubate for up to 30 minutes, followed by a 20-second 
hematoxylin counter-stain.  The use of either counterstain should give you good 
cytological detail, with blue-green melanin and brown DAB specific signal.



Thanks in advance,

Naira



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[Histonet] Slide Hybridizer

[Houston, Ronald]

If anyone has a used slide Hybridizer, e.g. Dako or Spot-Light Slide Hybridizer 
(Invitrogen) or StatSpin ThermoBrite* Slide Hybridizer, for sale please let me 
know.

Thanks

Ronnie Houston, MS HT(ASCP)QIHC
Anatomic Pathology Manager

ChildLab, a Division of Nationwide Children's Hospital

www.childlab.com


700 Children's Drive
Columbus, OH 43205
(P) 614-722-5450
(F) 614-722-2899
ronald.hous...@nationwidechildrens.orgmailto:ronald.hous...@nationwidechildrens.org
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~ E.M. Forster



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RE: [Histonet] QIHC

[Houston, Ronald]

Read and digest the Dako book and you will sail through the exam. For a 
specialist type qualification it really is pitiful.

Ronnie 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Michael Bradley
Sent: Wednesday, February 09, 2011 4:30 PM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] QIHC

Hi all

I am preparing to take the QIHC exam.  Does anyone have any suggestions on
what I should study?  What areas does the exam concentrate on and what
research material I can use.

Thanks
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RE: [Histonet] Bond Refine Red for manual IHC

[Houston, Ronald]

Yes, we've used both the AP Red and DAB Refine kits manually with comparable 
results to the automation

Ronnie Houston
Anatomic Pathology Manager
Nationwide Children's Hospital
Columbus OH 43205
(614) 722 5450
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sarka Lhotak
Sent: Friday, February 04, 2011 10:18 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Bond Refine Red for manual IHC

Hello all,

I wonder if anyone is using the Bond Polymer Refine Red Detection kit
(cat # DS390, Leica) for manual IHC. I've seen some beautiful staining,
the technical specialist at Leica, however, told me it is only for
Leica automatic stainers. It is an AP stain. 
Has anybody tried it?

Thanks a lot,

Sarka Lhotak
McMaster University
Hamilton, Ontario, Canada

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[Histonet] RE: g ratio

[Houston, Ronald]

Michel,

Most studies involving morphometric studies of peripheral nerves involve 
thick plastic sections stained with toluidine blue. In my opinion, you are 
unlikely to get consistent and acceptable staining results on paraffin sections 
for the simple reason that the intense blue staining on thick EM sections is 
more from the osmium tetroxide than the toluidine blue per se.




Ronnie Houston, MS HT(ASCP)QIHC
Anatomic Pathology Manager

ChildLab, a Division of Nationwide Children's Hospital

www.childlab.com


700 Children's Drive
Columbus, OH 43205
(P) 614-722-5450
(F) 614-722-2899
ronald.hous...@nationwidechildrens.orgmailto:ronald.hous...@nationwidechildrens.org
www.NationwideChildrens.orghttp://www.NationwideChildrens.org

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bataillon Michel
Sent: Tuesday, January 25, 2011 5:16 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] g ratio



Hello,

We have to measure the g ratio (ratio of the inner axonal diameter to the 
total outer diameter) - rat sciatic nerve - at least 500 myelinated fibers per 
nerve will be evaluated with a standard imaging soflware.

Do you think that we can perform this work with thin paraffin slides - 1.5µm 
(stained with toluidine blue), in spite of the best practice seems to prepare 
semithin sections from epoxy resin blocks.

Thank you,

Michel



Michel BATAILLON

Histology

Evreux, France



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RE: [Histonet] C3d

[Houston, Ronald]

Richard,

We have used the C3d from Cell Marque for quite some time on our Bonds with 
very clean crisp results on all our transplant biopsies. The jury is still out 
on how much more information we get in addition to C4d.

Ronnie

Ronnie Houston, MS HT(ASCP)QIHC
Anatomic Pathology Manager

ChildLab, a Division of Nationwide Children's Hospital

www.childlab.com


700 Children's Drive
Columbus, OH 43205
(P) 614-722-5450
(F) 614-722-2899
ronald.hous...@nationwidechildrens.orgmailto:ronald.hous...@nationwidechildrens.org
www.NationwideChildrens.orghttp://www.NationwideChildrens.org

One person with passion is better than forty people merely interested.
~ E.M. Forster



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Richard Cartun
Sent: Thursday, January 20, 2011 12:26 PM
To: Histonet
Subject: [Histonet] C3d



Is anyone using a Commercially-available C3d (not CD3) antibody for 
identifying antibody-mediated rejection in formalin-fixed cardiac transplant 
tissue?  Thanks.



Richard



Richard W. Cartun, MS, PhD

Director, Histology  Immunopathology

Director, Biospecimen Collection Programs

Assistant Director, Anatomic Pathology

Hartford Hospital

80 Seymour Street

Hartford, CT  06102

(860) 545-1596 Office

(860) 545-2204 Fax







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[Histonet] RE: Myocardial Biopsies

[Houston, Ronald]

7 slides, one ribbon of 6 sections/slide: HE x4, C4d x1, Masson Trichrome x1, 
unstained x1

Ronnie Houston
Anatomic Pathology Manager
Nationwide Children's Hospital
Columbus OH 43205
(614) 722 5450

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of 
jeffery.mil...@spectrum-health.org
Sent: Thursday, January 20, 2011 12:06 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Myocardial Biopsies

My hospital has recently started performing heart transplants and we are now 
receiving numerous myocardial biopsies to check for rejection. One of our 
forensic pathologists is an expert in cardiovascular pathology and has read 
many biopsies over the years for other hospitals. His protocol for cutting 
these biopsies is very labor intensive (10 slides with 8 serial sections on 
each slide, some stained with HE others kept unstained and 2 for C4d immunos). 
 I'm curious to know what other labs are doing for these biopsies in hopes of 
getting this protocol changes.

Thanks for your help

Jeff Miller
Spectrum Health
Grand Rapids. MI
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RE: [Histonet] Courier Tracking Mechanism

[Houston, Ronald]

Our lab here, and previous employ in Virginia, uses Gajema

Ronnie Houston
Anatomic Pathology Manager
Nationwide Children's Hospital
Columbus OH 43205
(614) 722 5450

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Nita Searcy
Sent: Thursday, January 20, 2011 1:16 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Courier Tracking Mechanism

Am looking for a method for verification of specimens for couriers/ 
laboratories. Anyone use such an animal?


Bill, as I recall you have in use such an animal?

Thanks
Nita



Nita Searcy, HT/HTL (ASCP)
Scott and White Hospital
Division Manager, Anatomic Pathology
2401 S. 31st. Street 
254-724-2438
Temple, Texas, 76502
nsea...@swmail.sw.org


254-724-2438

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RE: [Histonet] C3d

[Houston, Ronald]

Pam, 
We have been performing C4d by IHC for about 3 years now and C3d for many 
months on all our transplant biopsies. Obviously, being able to do this on 
paraffin obviates the need to have additional tissue for frozens (although I do 
understand the need for additional IF on kidney biopsies - but we're working on 
that!). 
Currently we get both antibodies from Cell Marque and run them on our Bonds
Let me know if you need any more info.
Ronnie

Ronnie Houston
Anatomic Pathology Manager
Nationwide Children's Hospital
Columbus OH 43205
(614) 722 5450

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Pamela Marcum
Sent: Thursday, January 20, 2011 1:28 PM
To: Richard Cartun
Cc: Histonet
Subject: Re: [Histonet] C3d



Hi Dr Cartun, 



I hope anyone answering this will make it available for all of HistoNet.  I 
would be very interested in the use of this antibody as we are already using 
C4d IF for kidney here. 



Pamela Marcum 

UAMS 

Little Rock AR 


- Original Message - 
From: Richard Cartun rcar...@harthosp.org 
To: Histonet histonet@lists.utsouthwestern.edu 
Sent: Thursday, January 20, 2011 11:26:29 AM 
Subject: [Histonet] C3d 

Is anyone using a Commercially-available C3d (not CD3) antibody for 
identifying antibody-mediated rejection in formalin-fixed cardiac transplant 
tissue?  Thanks. 

Richard 

Richard W. Cartun, MS, PhD 
Director, Histology  Immunopathology 
Director, Biospecimen Collection Programs 
Assistant Director, Anatomic Pathology 
Hartford Hospital 
80 Seymour Street 
Hartford, CT  06102 
(860) 545-1596 Office 
(860) 545-2204 Fax 



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- Original Message - 
From: Richard Cartun rcar...@harthosp.org 
To: Histonet histonet@lists.utsouthwestern.edu 
Sent: Thursday, January 20, 2011 11:26:29 AM 
Subject: [Histonet] C3d 

Is anyone using a Commercially-available C3d (not CD3) antibody for 
identifying antibody-mediated rejection in formalin-fixed cardiac transplant 
tissue?  Thanks. 

Richard 

Richard W. Cartun, MS, PhD 
Director, Histology  Immunopathology 
Director, Biospecimen Collection Programs 
Assistant Director, Anatomic Pathology 
Hartford Hospital 
80 Seymour Street 
Hartford, CT  06102 
(860) 545-1596 Office 
(860) 545-2204 Fax 



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RE: [Histonet] Billing question

[Houston, Ronald]

We've been repeatedly told by Compliance that if a pathologist (or a clinician) 
orders a test, the test result must be included in the report and the test must 
be billed for; even if the result is non-contributory.


Ronnie Houston
Anatomic Pathology Manager
Nationwide Children's Hospital
Columbus OH 43205
(614) 722 5450

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Drew Meyer
Sent: Thursday, January 06, 2011 12:40 PM
To: Weems, Joyce
Cc: Histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Billing question

Hey Joyce,

My understanding has always been that if the pathologist specifically
mentions the stain in the report, then it's OK to charge all the fees.
However, if the pathologist chooses to leave any mention of the stain/immuno
in the report, then you can't bill for it.  So if the pathologist mentions a
specific stain was done, but that the findings were negative, you can still
charge.  In the case of an exhausted tumor, I think the ultimate discretion
comes to whether or not the pathologist chooses to mention it in the
report.  That's just from my experience, but I would love to hear how others
are handling instances such as these, too!

Drew

On Thu, Jan 6, 2011 at 12:22, Weems, Joyce jwe...@sjha.org wrote:

 When you have performed a special stain or immuno that does not help the
 pathologist (tumor exhausted, etc) do you still charge for the technical
 component and the pathologist credits the professional fee? Just curious..

 Happy New Year, Everyone!

 Thanks! j


 Joyce Weems
 Pathology Manager
 Saint Joseph's Hospital
 5665 Peachtree Dunwoody Rd NE
 Atlanta, GA 30342
 678-843-7376 - Phone
 678-843-7831 - Fax


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