Re: [Histonet] Hiring Lead Histotechnician

[Jeffrey Robinson via Histonet]

We have pretty much given up on finding certified techs willing to relocate (we 
are in central California).  We have mostly turned to trainees from within our 
lab.  We have actually found some very good techs that way.  And then there are 
always the other local labs that are willing to pay more siphoning off 
experienced techs.  Not an easy task these days to find qualified 
personnel.  Jeff Robinson, Senior Histotechnologist, Sierra Pathology Lab, 
Clovis, CA.

-Original Message-
From: Stephanie L. Thompson via Histonet 
Sent: Wednesday, July 6, 2022 9:51 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Hiring Lead Histotechnician

⚠EXTERNAL EMAIL⚠ Look for suspicious attachments/links. [X]  EXTERNAL EMAIL [X] 
Do not open attachments or click on links unless you recognize the sender and 
know the content is safe.

Does anyone have an idea on why I can't get anyone interested in working for 
our lab in Exeter, NH?

Are there any other avenues to search for candidates that everyone could share. 
I have turned over every rock I can find!

sthomps...@sonichealthcareusa.com
M:210-428-1646
This message contains privileged and confidential information intended only for 
the use of the addressee named above. If you are not the intended recipient of 
this message you must not disseminate, copy, or take any action in reliance on 
it.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


This email and attachments may contain PHI that is privileged and confidential 
and is not intended for any unauthorized person. If you, the reader, are not 
the intended recipient you are hereby notified that any dissemination, 
distribution or copying of this communication is strictly prohibited. Do not 
read the email but instead reply to the sender and destroy the message and any 
attachments. Thank you.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Ventana Retic problems

[Jeffrey Robinson via Histonet]

Hi Donna-  I have been dealing with these same exact issues for 3 1/2 years now 
so I think I have achieved "expert status" in dealing with this problem.

First, I'll give you my current protocol.  When all things are working 
smoothly, it produces a nice stain.  But it is very frustrating when it starts 
to "fade."

We cut all retics at 5.  Thinner cuts will definitely look lighter.

Protocol:  warmup slide:  47 degrees C.
 Oxidizer:4 minutes
 Decolorizer: 4 minutes
 Sensitizer:  8 minutes
 Optimize Counterstain Intensity:  4 Minutes

I have gone around and around with Ventana on decontamination problems with 
this instrument.  I was performing full decontamination runs every month.  My 
rep finally said to just decon the bulk wash carboy and the wash bottle on the 
instrument when fading begins to show.  Ventana claims there will be a new wash 
out this year that will hopefully take care of the problem once and for all but 
no one at Ventana can give me a release date on that.
In the meantime, this is what I do:

Daily:  run the "Purge Wash" function test 3 times in the morning before 
running any slides.  Be sure to run the "Purge" function and not the "Prime" 
function.
When loading slides, put all of your retic slides on after everything else 
(after the GMS, etc., slides).

When staining starts to fade:  after ruling out "thin cuts" and other obvious 
problems it is time for decon.  The fading will show first on the patient 
tissue (the control may still look OK).
Here is my current decon protocol:  I use the Lysol IC decon solution. I have 
an extra 6 gallon carboy and I leave some made up (diluted) so that it is ready 
to go.  Put some decon solution on a 4X4 guaze and wipe the dispenser tip 
underneath the top (lift lid up for access).  Dump the wash solution in the 
wash bottle on the instrument.  Rinse out with DI water. Fill with decon 
solution.  Swish solution inside bottle.  Replace bottle (on instrument).  Run 
"Purge Wash" function test (3 times).  After Purge #3, let the solution sit in 
the lines for at least 15 minutes (the longer the better).  When you are ready 
to proceed, rinse bulk bottle well several times and replace with DI water.  
Run "Purge Wash" 3 more times.  Time is not a factor here so you can just run 
them one after another.  After the third purge, replace the DI with BM SS wash. 
 Run "Purge Wash" function test 3 more times.  That's it.  I find I need to run 
this procedure about once a month.
Additionally, I decon the 5 gallon bulk wash carboy EVERY TIME I make up a new 
batch.  It doesn't take long (I just let the decon solution sit for 15 minutes) 
and then when I do need to do the modified decon on the instrument I do not 
need to worry about the bulk carboy.

I hope this helps- it takes a little time but it does seems to help with the 
retic stain intensity.

Jeff Robinson, Senior Histotechnologist (HT, HTL), Sierra Pathology Lab, 
Clovis, CA.

-Original Message-
From: donna mihalik rossi via Histonet 
[mailto:histonet@lists.utsouthwestern.edu]
Sent: Wednesday, June 14, 2017 1:45 PM
To: histonet
Subject: [Histonet] Ventana Retic problems

Hi Histonetters!  We are experiencing sporadic staining of retic fibers from 
our Ventana Benchmark machines.  We have 2 machines and are having the same 
problem on both. The machines were both decontaminated 2 weeks ago with all new 
solutions  being made.  The fibers are not crisp and are disconnected.  Our 
control tissue is  at the top of the slide with the patient tissue at the 
bottom. It appears that the control is staining better than the patient tissue 
but still is  not crisp. We have tried different lots with the same result. Any 
comments before we consult Ventana? Your help would be appreciated.  Thanks, 
Donna  Rossi, PSU


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


This email and attachments may contain PHI that is privileged and confidential 
and is not intended for any unauthorized person. If you, the reader, are not 
the intended recipient you are hereby notified that any dissemination, 
distribution or copying of this communication is strictly prohibited. Do not 
read the email but instead reply to the sender and destroy the message and any 
attachments. Thank you.

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] square footage for formalin

[Jeffrey Robinson via Histonet]

Hi Alison-  many years ago I worked in a hospital.  We had a very small Frozen 
Section room in the OR.  We did keep a 5 gallon carboy of formalin in there for 
the convenience of the OR staff so they would have formalin available for their 
specimens.  We were told by someone in "authority" (sorry, can't remember who) 
that we needed to switch to 1 gallon formalin bottles in the FS room to lessen 
the chance of a spill.  As "luck" would have it, someone on the OR staff opened 
the last 5 gallon carboy before the switch went into effect and left it on its 
side. The spigot was barely open and the entire 5 gallons leaked out during the 
night.  They had to call in a HazMat team for cleanup as the fumes were 
overwhelming.  Speaking from experience, I would highly recommend switching 
over to 1 gallon bottles of formalin for OR use.  Jeff Robinson, Senior 
Histotechnologist, Sierra Pathology Lab, Clovis, CA.

-Original Message-
From: Eck, Allison via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Monday, February 27, 2017 11:27 AM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] square footage for formalin

Good afternoon,
We have inspectors here and they are questioning the size of the room in the 
operating room where they keep their 5 gallon cube.  Does anyone know of any 
square footage requirements for a room that where formalin is kept and used?

Thank you in advance
Allison

Allison Eck, HTL(ASCP)cm,QLS, AHI(AMT)
Lead Tech Histology
Doylestown Hospital
595 W State St
Doylestown, PA 18901
215-345-2264
a...@dh.org


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


This email and attachments may contain PHI that is privileged and confidential 
and is not intended for any unauthorized person. If you, the reader, are not 
the intended recipient you are hereby notified that any dissemination, 
distribution or copying of this communication is strictly prohibited. Do not 
read the email but instead reply to the sender and destroy the message and any 
attachments. Thank you.

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] link was lost

[Jeffrey Robinson via Histonet]

Subscribe, please.  Jeff Robinson, Sierra Pathology Lab, Clovis, CA.


This email and attachments may contain PHI that is privileged and confidential 
and is not intended for any unauthorized person. If you, the reader, are not 
the intended recipient you are hereby notified that any dissemination, 
distribution or copying of this communication is strictly prohibited. Do not 
read the email but instead reply to the sender and destroy the message and any 
attachments. Thank you.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] MCH IHC

[Jeffrey Robinson via Histonet]

Hi Estela-  I had the same problem with the Bond multicytokeratin.  I currently 
have 3 vials of the "old" one on backorder.  I had to validate the new 
"reformulation" as I have now run out of the old one (PA0909).  Have your rep 
send you the new one (PA0012).  My rep sent me one for free.  It seems to be a 
bit stronger than the old one but I ended up using the same protocol (ER2 for 
20 minutes).  CAUTION!!!  If you do not have the newest database downloaded 
onto your instrument you will need to get that done.  If you have the old 
version 4.0 software like I do it is a different database.  Call Leica tech 
support for help as you may CRASH YOUR DATABASE if you download it incorrectly. 
 I needed Database 67.
Jeff Robinson, Senior Histotechnologist, Sierra Pathology Lab, Clovis, CA.

-Original Message-
From: Estela Martinez via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Thursday, July 21, 2016 8:54 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] MCH IHC

Hello My Histo Friends!!

Does anybody have an extra MCK RTU Antibody-- Catalog No. PA0909-- Clone AE1 
and AE3, for the Leica Bond Max machine. that we can borrow until we get ours 
in.  Apparently it's been back ordered til Aug 1 and we are out.  Please let me 
know if someone can help us out.  Thank you in advance!!


Estela Martinez
Histology Supervisor
Medical Center Hospital
Odessa, TX 79761
432-640-2348
emartin...@echd.org
CONFIDENTIALITY NOTICE: The documents accompanying this email transmission 
contain confidential information belonging to the sender that is legally 
privileged. This information is intended only for the use of the individual or 
entity named above. The authorized recipient of this information is prohibited 
from disclosing this information to any other party without permission of 
original user and is required to destroy the information after its stated need 
has been fulfilled. If you are not the intended recipient, you are hereby 
notified that any disclosure, copying, distribution, or action taken in 
reliance on the contents of these documents is strictly prohibited. If you have 
received this email in error, please notify the sender immediately to arrange 
for return of these documents.

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


This email and attachments may contain PHI that is privileged and confidential 
and is not intended for any unauthorized person. If you, the reader, are not 
the intended recipient you are hereby notified that any dissemination, 
distribution or copying of this communication is strictly prohibited. Do not 
read the email but instead reply to the sender and destroy the message and any 
attachments. Thank you.

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] PAX 2

[Jeffrey Robinson via Histonet]

Good Morning-  can anyone give me a recommendation for a predilute PAX2 
antibody?  The one I was using has been discontinued and the replacement 
product does not seem to perform very well.  I will be using it on the Bond 
III.  Thanks in advance!
Jeff Robinson, Senior Histotechnologist, Sierra Pathology Lab, Clovis, CA.


This email and attachments may contain PHI that is privileged and confidential 
and is not intended for any unauthorized person. If you, the reader, are not 
the intended recipient you are hereby notified that any dissemination, 
distribution or copying of this communication is strictly prohibited. Do not 
read the email but instead reply to the sender and destroy the message and any 
attachments. Thank you.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] floor vibration

[Jeffrey Robinson via Histonet]

An additional word of caution about placing the EM Lab under ANY kind of 
potential water leak.  Tim Morken and I can attest to that!  They installed a 
new CT scanner in Radiology directly above the EM Lab.  They broke some sort of 
water pipe during the installation and it leaked so much that part of the 
ceiling collapsed onto the scope!  They didn't bother checking to see if there 
was any damage on the next floor down- they just left.  Tim had a huge surprise 
waiting for him the next morning.  We always had to cover the scope with a 
sheet of plastic at night after that in case they installed something else.  
Jeff Robinson, Senior Histotechnologist, EM Tech (emeritus), Sierra Pathology 
Lab, Clovis, CA.

-Original Message-
From: Keyser Gerald T via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Monday, May 16, 2016 1:25 PM
To: 'Nancy Schmitt'; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] floor vibration

I can think of two things.

First, relentlessly make fun of your building planner for putting a histology 
lab underneath a laundry. This is a mistake worthy of pointing and laughing.

Second, there are isolation tables and platforms. That's probably the first 
thing I would try.

http://www.taab.co.uk/pdf-details/305_taab_products_1402580607.pdf


Gerry

-Original Message-
From: Nancy Schmitt via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Monday, May 16, 2016 10:03 AM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] floor vibration

Happy Monday!
We are moving to a new space and part of our area is above the laundry - there 
is some vibration from there.  Does anyone have any experience with this and 
could you please share how you accommodated this?  Special flooring, pads, etc.
Thank you much!

Nancy Schmitt HT, MLT (ASCP)

NOTICE: This email may contain legally privileged information. The information 
is for the use of only the intended recipient(s) even if addressed incorrectly. 
If you are not the intended recipient, please notify the sender that you have 
received it in error and then delete it along with any attachments. Thank you.




NOTICE: This email may contain legally privileged information. The information 
is for the use of only the intended recipient(s) even if addressed incorrectly. 
If you are not the intended recipient, please notify the sender that you have 
received it in error and then delete it along with any attachments. Thank you.


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


This email and attachments may contain PHI that is privileged and confidential 
and is not intended for any unauthorized person. If you, the reader, are not 
the intended recipient you are hereby notified that any dissemination, 
distribution or copying of this communication is strictly prohibited. Do not 
read the email but instead reply to the sender and destroy the message and any 
attachments. Thank you.

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Melanin Bleach

[Jeffrey Robinson via Histonet]

Hi Karen-  I have also had problems getting tissue being stained for IHC 
markers to adhere to the slide.  The Potassium permanganate is really harse on 
the tissue (if you can get the sections to stay on).  Here is a trick I picked 
up at an NSH lecture that I have used successfully several times.

Run your IHC stains as usual.  Rinse well in water.  Do not counterstain with 
hematoxylin.  Stain with DiffQuik II for 30 seconds.  Rinse in water for a 
short period of time.  Differentiate with 10 dips each in 2 changes of 95% ETOH 
and 2 changes of 100% ETOH and then clear in xylene and coverslip.
Results:  melanin pigment will turn green.  Brown DAB stain will be unaffected. 
 Other tissue elements will be stained a medium blue color.
This method will also work for other Special Stains but I have not attempted to 
modify this method for use on "Red" stains.

Jeff Robinson, Senior Histotechnologist, Sierra Pathology Lab, Clovis, CA.

-Original Message-
From: Heckford, Karen - SMMC-SF via Histonet 
[mailto:histonet@lists.utsouthwestern.edu]
Sent: Tuesday, May 10, 2016 8:45 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Melanin Bleach

Good Morning,
I need some help.  Yesterday I bleached some heavily pigmented tissue.  I have 
to run some IHC's on them.   I bought the bleaching kit from American Master 
Tech.  I had to put the Permanganate for about 6 hours to get the melanin and 
then a couple of minutes in Oxalic Acid.  I had to let them set overnight 
because I could not get another IHC run in that day.   It looks like the tissue 
fell off during decloaking.I used Apex slides.   I rarely ever have to 
bleach anything here.  So I am not sure if I did this correctly.   I am 
thinking I need to bleach and do the run in the same day and not let them set 
over night in DiH20.

Thanks,


Karen Heckford HT ASCP CE
Lead Histology Technician
St. Mary's Medical Center
450 Stanyan St.
San Francisco, Ca. 94117
415-668-1000 ext. 6167
karen.heckf...@dignityhealth.org

  Caution:  This email message, including all content and attachments, is 
CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED.  The 
information contained in this email message is intended only for the use of the 
recipient(s) named above. If the reader of this message is not the intended 
recipient or an agent responsible for delivering it to the intended recipient, 
you have received this document in error.  Any further review, dissemination, 
distribution, or copying of this message is strictly prohibited.  If you have 
received this communication in error, please notify us  immediately by reply 
email.  Thank you."



___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


This email and attachments may contain PHI that is privileged and confidential 
and is not intended for any unauthorized person. If you, the reader, are not 
the intended recipient you are hereby notified that any dissemination, 
distribution or copying of this communication is strictly prohibited. Do not 
read the email but instead reply to the sender and destroy the message and any 
attachments. Thank you.

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] GLOMERULI ADEQUACY

[Jeffrey Robinson via Histonet]

Hi Melissa-  when Tim Morken taught me to be an EM tech many moons ago we would 
take a small dissecting microscope to the CT room.  We would have the 
radiologist place the first core on a sterile tongue depressor and then we 
would check it in some saline on a dental wax square under the scope.  The 
viable gloms were quite easy to see.  We could ask for an additional core or 
two right then if we felt it was needed.  We would then place the cores in a 
saline vial and take them back to our lab to split up the cores into the 
appropriate fixatives.  It really was quite valuable to have that interaction 
with the radiologists and to have the ability to ensure that we were obtaining 
adequate samples with the dual goals of giving ourselves enough tissue to 
perform the EM workup as well as avoiding any repeat biopsies due to inadequate 
sampling.

Jeff Robinson, Senior Histotechnologist, EM Tech Emeritus, Sierra Pathology 
Lab, Clovis, CA.

-Original Message-
From: Melissa Likens via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Wednesday, April 13, 2016 7:22 AM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] GLOMERULI ADEQUACY

I have a question about how other institutions handle microscopic evaluation of 
glomeruli adequacy in renal specimens?  Specifically, who at you looks at the 
cores to determine if glomeruli are present before submitting specimens for 
further testing?  Do the pathologists look at them? Radiologists performing the 
cores?  Other staff?
Also, any links or recommendations for training for evaluating renal biopsies 
for glomeruli would be appreciated.
Thanks, Melissa
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


This email and attachments may contain PHI that is privileged and confidential 
and is not intended for any unauthorized person. If you, the reader, are not 
the intended recipient you are hereby notified that any dissemination, 
distribution or copying of this communication is strictly prohibited. Do not 
read the email but instead reply to the sender and destroy the message and any 
attachments. Thank you.

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] SOX 10 Cellmarque

[Jeffrey Robinson via Histonet]

Hi Edison-  I have had some headaches getting the SOX-10 to run in the past but 
I now have all of the kinks ironed out and it works quite well.  I had started 
with the CellMarque mouse monoclonal of their RTU SOX-10.  I had it working 
well and then Leica recalled their red detection kits.  I could never get the 
mouse monoclonal to work again after that with the new red detection kits.  
BioCare came out with a RTU rabbit monoclonal so I tried that and Bingo!- great 
staining once again.  CellMarque has since come out with their own RTU rabbit 
monoclonal.  They sent me a sample to try and that also stained well but I had 
already validated the BioCare antibody so I have stayed with that.  I run it 
with ER2 for 30 minutes with the red kit.  A word of caution:  do not delay 
when taking those slides off the Bond.  Take them off as soon as they are 
finished, rinse in DI water for a short period of time, run down through the 
alcohols (I use one 95% and 2 100% ETOH), into the xylene and covers
 lip immediately.  The red stain will "wash" out if you delay running the 
slides down.  This cannot be repaired to the best of my knowledge- you will 
have to rerun new slides.  I use this same protocol for Melan A and it also 
works great.  I use the Leica Melan A (RTU).  Good Luck!

Jeff Robinson, Senior Histotechnologist, Sierra Pathology Lab, Clovis, CA.

-Original Message-
From: Edison Narvaez via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Thursday, January 28, 2016 6:13 AM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] SOX 10 Cellmarque

Hello,
Anybody running Cellmarque's RTU SOX 10 antibody on the bond III, any protocol 
suggestions, I would like to run this antibody with Leica's red kit.


Thank you





The contents of this message, together with any attachments, are intended only 
for the use of the person(s) to which they are addressed and may contain 
confidential and/or privileged information. Further, any medical information 
herein is confidential and protected by law. It is unlawful for unauthorized 
persons to use, review, copy, disclose, or disseminate confidential medical 
information. If you are not the intended recipient, immediately advise the 
sender and delete this message and any attachments. Any distribution, or 
copying of this message, or any attachment, is prohibited.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


This email and attachments may contain PHI that is privileged and confidential 
and is not intended for any unauthorized person. If you, the reader, are not 
the intended recipient you are hereby notified that any dissemination, 
distribution or copying of this communication is strictly prohibited. Do not 
read the email but instead reply to the sender and destroy the message and any 
attachments. Thank you.

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] PAX-5 staining problems

[Jeffrey Robinson via Histonet]

Good Morning to all:  I am having staining problems with my PAX-5 predilute 
from CellMarque.  I was wondering if anyone else has experienced a dramatic 
dropoff in staining signal with this antibody after a few months.  I have now 
had this happen to me twice in the last 3 months.  It stains very nicely when 
the vial is first opened and seems to hold staining intensity for about 3 
months and then drops off dramatically even though the expiration date is 2 
years out.  I am using it on the Leica Bond.  I have been in contact with 
CellMarque about this problem but they tell me they have not had any complaints 
from any other labs.  I use many antibodies from CellMarque and have only seen 
this problem occur with one other antibody (ALK-1) about a year ago.  If you 
are experiencing this problem in your lab please contact Liz Beitman at 
CellMarque (lbeit...@cellmarque.com) so that 
they can have all available information to further research this problem.  
Thank 
 you!
Jeff Robinson, Senior Histotechnologist, Sierra Pathology Lab, Clovis, CA.


This email and attachments may contain PHI that is privileged and confidential 
and is not intended for any unauthorized person. If you, the reader, are not 
the intended recipient you are hereby notified that any dissemination, 
distribution or copying of this communication is strictly prohibited. Do not 
read the email but instead reply to the sender and destroy the message and any 
attachments. Thank you.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Retics on Ventana Benchmark Special Stainer

[Jeffrey Robinson via Histonet]

Good Morning to all Histotechs-  There have been many questions over the past 
several months regarding poor retic staining on the Ventana Benchmark Special 
Stainer.  With the help of my histotech friends, I have now achieved successful 
staining on both liver and bone marrow specimens utilizing this instrument.
My primary source of protocol information has been Heather Nowacki at the Mayo 
Clinic.  Heather says that they use B-5 fixative and RDO Gold decalcifier on 
their bone marrow cores.  We use B Plus fixative and Immunocal  decalcifier so 
the choice of an appropriate fixative and decalcifier does not seem to be a 
concern in the ability of this instrument to achieve the desired retic staining 
intensity.

Here is Heather's protocol for retic:  Heat:  45 degrees C.
 
Oxidizer:  4 minutes
 
Decolorizer: 4 minutes
 
Sensitizer:  8 minutes
 
Silver:  12 minutes
 
Nuclear Fast Red Counterstain:  4 minutes

I did not need to modify any of Heather's protocol options.  I had previously 
been unable to achieve any satisfactory staining on bone marrow retic staining 
on the Benchmark Special Stainer.  I showed my bone marrow retic test slides to 
one of my pathologists and he rated them comparable to the retic slides we are 
currently staining on the old Ventana Nexes stainer.

Notes:  It seems that the primary issue affecting proper retic staining on the 
Benchmark Special Stainer has been the lack of sufficient heat applied to the 
slides during the staining run.  Ventana has released a software upgrade that 
allows for a much wider range of heating temperature options during retic 
staining.  You will need to have the new software upgrade installed by Ventana 
before you will be able to use this protocol.  Ventana also discussed an 
enhanced decontamination protocol for the Benchmark Special Stainer that 
requires some additional reagents but the Ventana technical rep who installed 
my upgrade determined that the enhanced decontamination protocol was not 
necessary in our lab.  She felt that this enhanced protocol would only be 
beneficial in labs that have had major contamination issues (including Special 
Stains Wash pH changes) in the past.  We cut all of our retic slides at 5 (even 
for the Nexes) and I have been quite happy with the staining intensity on slides
  cut at that thickness.  Slides cut at 3 or 4 stain too light for my taste.

Acknowledgements:  I would like to thank Tim Morken for spotting the Mayo 
Clinic poster on Ventana Benchmark Special Stainer Retic staining at NSH last 
month.  I would also like to thank the team at the Mayo Clinic who worked on 
this project allowing the rest of us in the field to benefit from their hard 
work.  The Mayo team includes:  Heather Nowacki, HTL;  Mark Keith, HTL;  Scott 
Antilla, HTL;  and Joaquin Garcia, M.D.

I hope this information is of use to my fellow histotechs who have the Ventana 
Benchmark Special Stainer in their labs.

Jeff Robinson, HT, HTL, Senior Histotechnologist, Sierra Pathology Lab, Clovis, 
CA.


This email and attachments may contain PHI that is privileged and confidential 
and is not intended for any unauthorized person. If you, the reader, are not 
the intended recipient you are hereby notified that any dissemination, 
distribution or copying of this communication is strictly prohibited. Do not 
read the email but instead reply to the sender and destroy the message and any 
attachments. Thank you.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] are you desperate enough to hire a B.Sc. graduate?

[Jeffrey Robinson via Histonet]

I would love to have some talented staff with degrees!  I have a staff of 
around 22 people but I am currently the only HTL (I have a BA in Biology).  I 
do not have anyone else who is even eligible to sit for the HTL exam.  It would 
be very nice to have someone to share the load with!
A friend of mine went to civil engineering school.  He said that firms would 
hire engineers for management positions because it was a whole lot easier to 
teach an engineer the fine points of management than it was to teach 
engineering to a manager!  The same applies to Histology- it is a lot easier to 
teach Histology to someone who already has a science background.  I currently 
have one trainee who has taken many college science courses and his training 
has been a breeze so far.
Jeff Robinson, Senior Histotechnologist, Sierra Pathology Lab, Clovis, CA.

-Original Message-
From: Michael LaFriniere via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Monday, August 10, 2015 11:39 AM
To: tgen...@gmail.com
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] are you desperate enough to hire a B.Sc. graduate?

Yes this is a common problem, although I am not sure desperate enough would 
be appropriate  verbiage.  As histologists over the years we have learned to 
cope with and being highly resourceful. I have also resorted to the same idea, 
hiring both AS and BS individuals filling positions and training specific 
technical task to meet the production needs at present has worked very well in 
my laboratory.

Michael
Michael R. LaFriniere, HT (ASCP)
Executive Director


Capital Choice Pathology Laboratory
12041 Bournefield Way, Suite A . Silver Spring, MD 20904
P: 240.471.3427 . F: 240.471.3401 . Cell 410-940-8844 
michael.lafrini...@ccplab.com



-Original Message-
From: Tyrone Genade via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Monday, August 10, 2015 12:13 PM
To: histonet
Subject: [Histonet] are you desperate enough to hire a B.Sc. graduate?

Hello,

Last week a I visited Pittsburgh and had a chance to talk with a fellow 
histologist there. He remarked on the dire state his lab is in with respect to 
finding qualified histologists to employ; and that the lab was now considering 
hiring B.Sc. graduates and training them up in sectioning etc... Is this a 
common problem, the trouble finding qualified histotechs, and are other labs 
also considering hiring B.Sc. graduates to staff their labs?

I have one student working with me (with medical school aspirations) who 
sections beautifully. There is surely talent out there...

Bye
--
Tyrone Genade
Orange City, Iowa
tel: (+1) 712 230 4101
http://tgenade.freeshell.org

Romans 6:23: The gift of God is eternal life through Christ Jesus our Lord.
To find out how to receive this FREE gift visit http://www.alpha.org.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


This email and attachments may contain PHI that is privileged and confidential 
and is not intended for any unauthorized person. If you, the reader, are not 
the intended recipient you are hereby notified that any dissemination, 
distribution or copying of this communication is strictly prohibited. Do not 
read the email but instead reply to the sender and destroy the message and any 
attachments. Thank you.

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Spirochete Control Block?

[Jeffrey Robinson via Histonet]

Hi Brian-  we just buy controls for spirochetes.  I did procure a control block 
many years ago from someone working with pigs in a vet histology facility in 
Missouri.  It was quite good as I recall.  That may be an option for you.  We 
run so few Steiners that it is not worthwhile for me to try and find a control 
block.  Good Luck!
Jeff Robinson, Senior Histotechnologist, Sierra Pathology Lab, Clovis, CA.

-Original Message-
From: Cooper, Brian via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Wednesday, August 05, 2015 11:57 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Spirochete Control Block?

Good afternoon Histonetters!

I'm fairly certain my chances of seeing a puppy dog, riding on the back of a 
unicorn, sitting under a blue moon are greater, but does anyone have a block of 
spirochetes they'd be willing to share? Recently, we've gotten some great 
controls from these posts and thought we'd give this a shot!  We're more than 
willing to trade!  Please contact me directly if you don't want to post your 
responses.

Thanks,

Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Department of Pathology and 
Laboratory Medicine Children's Hospital Los Angeles
4650 Sunset Blvd MS#43- Los Angeles, CA 90027
Ph: 323.361.3357 Pager: 213-209-0184
bcoo...@chla.usc.edumailto:bcoo...@chla.usc.edu



-
CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for 
the sole use of the intended recipient(s) and may contain confidential or 
legally privileged information. Any unauthorized review, use, disclosure or 
distribution is prohibited. If you are not the intended recipient, please 
contact the sender by reply e-mail and destroy all copies of this original 
message.

-

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


This email and attachments may contain PHI that is privileged and confidential 
and is not intended for any unauthorized person. If you, the reader, are not 
the intended recipient you are hereby notified that any dissemination, 
distribution or copying of this communication is strictly prohibited. Do not 
read the email but instead reply to the sender and destroy the message and any 
attachments. Thank you.

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Ventana H.Pylori antibody

[Jeffrey Robinson via Histonet]

Hi Julia-  I have had a lot of experience trying to get a clean H. pylori.  I 
don't recall using the Ventana H. pylori but I was using the CellMarque H. 
pylori for years.  It would often have a lot of background as you describe.  I 
run Ventana Benchmarks (XT and Ultra) and a Leica Bond.  The slides I would run 
on the Benchmarks were usually cleaner than those run on the Bond but still not 
as clean as I would like. I finally switched over to BioCare's H. pylori after 
many complaints from my pathologists.  It has been much cleaner on all of my 
IHC instruments.  Actually, the ones I run on the Ultra are the cleanest I have 
ever seen.  The slides run on the Bond may still have a little background but 
it is still much cleaner than the ones I used to run with the CellMarque 
antibody.  One of my pathologists who used to complain about the excessive 
background now calls it his go to stain and is very happy with how crisp it 
is. The organisms stand out very nicely.  BioCare will send you a 
 free sample if you want to try it.
Jeff Robinson, Senior Histotechnologist, Sierra Pathology Lab, Clovis, CA.

-Original Message-
From: Cates, Julia via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Thursday, July 30, 2015 8:30 AM
To: histonet-boun...@lists.utsouthwestern.edu; histonet@lists.utsouthwestern.edu
Subject: [Histonet] Ventana H.Pylori antibody

Good day Histonet,

I have seen in the past conversations regarding Ventana's H.Pylori antibody and 
how dirty looking the stain can be.  We have been using this antibody for 
several years now and have never really been happy with the quality.  We have 
tried the recommended methods to clean up the stain and still we run into 
repeating the stain and/or complaints from the pathologists.  We are 
considering using a different vendor but my concern is that my efforts will be 
a lateral move.  Is anyone using a product that produces a clean stain or is 
this something inherent to this antibody?  Are the pathologists happy with it 
and not just tolerating it?

Thank you for your suggestions,

Julia Cates, HT(ASCP)cm
Pathology Coordinator, Pathology
Florida Hospital Waterman
(352) 253- ext.4346 | Fax: (352) 253-3592

Confidentiality Statement: This email message, including any attachments, is 
for the sole use of the intended recipient and may contain confidential and 
privileged information. Any unauthorized review, use, disclosure, or 
distribution is prohibited.  If you are not the intended recipient, please 
contact the sender by reply to this email and delete the original and all 
copies of this email.


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


This email and attachments may contain PHI that is privileged and confidential 
and is not intended for any unauthorized person. If you, the reader, are not 
the intended recipient you are hereby notified that any dissemination, 
distribution or copying of this communication is strictly prohibited. Do not 
read the email but instead reply to the sender and destroy the message and any 
attachments. Thank you.

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Film Coverslip

[Jeffrey Robinson via Histonet]

We used an alternative tape (I believe it was from Mercedes) years ago and we 
had some major issues with the tape detaching from the slide over time (in 
storage) and taking the tissue on the slide with it.  Perhaps the xylene being 
dispensed needed to be adjusted or some other technical problem was in play 
(such as storage conditions) but we did not feel it was worth the risk.  We 
went back to the Sakura tape and as far as I know we have not had any more 
problems with detaching tape.
Jeff Robinson, Senior Histotechnologist, Sierra Pathology Lab, Clovis, CA.

-Original Message-
From: Carol Fields via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Friday, July 24, 2015 8:04 AM
To: Houston, Ronald
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Film Coverslip

We used Mercedes tape and it worked ok.  It is thicker and one pathologist 
preferred it.  The drawback was you had to keep the Coverslipper clean or it 
would get sticky and cause problems.
We had no staining issues.
Have a great weekend.
Carole


-Original Message-
From: Houston, Ronald via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Friday, July 24, 2015 7:21 AM
To: Rene J Buesa; Michael Kent
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Film Coverslip

At previous place of employment we used Statlab's tape - absolutely no problems 
with stain bleeding or fading, bubbles or peeling of the tape. There were no 
problems with the coverslipper either.

Of course Sakura is going to warn against using any other product but theirs. 
Consumables are how they stay in business.
Ronnie

-Original Message-
From: Rene J Buesa via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Friday, July 24, 2015 9:50 AM
To: Michael Kent; Histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Film Coverslip

If you are using the Sakura instrument, please do not use other film than 
Sakura's. It is not only much better but also will allow the coverslipper to 
work better.Sometimes a cheaper option will be more costly at the end.René


 On Friday, July 24, 2015 9:47 AM, Michael Kent via Histonet 
histonet@lists.utsouthwestern.edu wrote:


 Good morning, We are considering changing coverslip film for our high volume 
Sakura Prisma with coverslipper.  We have tested StatLab film and pathologists 
are fine, and have not seen media build up on the instrument.
Sakura is cautioning against alternatives for lamination and media build-up 
issues.  Any feedback from Histonetters will be appreciated.
Best and have a great weekend,
Mike
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonetd=BQIGaQc=FGzDrZ8hK6OoO1oc9Smc5viw6E0cF__gglPkcFwC2N8r=Sq5_V4WPe-NKHXWNZ6pAinmQolgHQEbnpaFk_iS9Rpap2gKCsp18_6Vj99Jv3oyZm=RufHkqFubCpo3-LgDyoM-j2btPBF8TK8U91KmVVuTQ4s=rDlVheZRVLr9DBjR0VW0ejS5nf827G5F3gzCYzHTdqge=



___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonetd=BQIGaQc=FGzDrZ8hK6OoO1oc9Smc5viw6E0cF__gglPkcFwC2N8r=Sq5_V4WPe-NKHXWNZ6pAinmQolgHQEbnpaFk_iS9Rpap2gKCsp18_6Vj99Jv3oyZm=RufHkqFubCpo3-LgDyoM-j2btPBF8TK8U91KmVVuTQ4s=rDlVheZRVLr9DBjR0VW0ejS5nf827G5F3gzCYzHTdqge=
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


This email and attachments may contain PHI that is privileged and confidential 
and is not intended for any unauthorized person. If you, the reader, are not 
the intended recipient you are hereby notified that any dissemination, 
distribution or copying of this communication is strictly prohibited. Do not 
read the email but instead reply to the sender and destroy the message and any 
attachments. Thank you.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

FW: [Histonet] Need Antibody (Bartonella/ Cat-Scratch) update

[Jeffrey Robinson]

Hi Sheila-  I received a reply from BioCare so I am passing the information 
along.  Jeff.

-Original Message-
From: Dax Arguello [mailto:dargue...@biocare.net] 
Sent: Thursday, April 02, 2015 11:46 AM
To: Jeffrey Robinson
Subject: RE: [Histonet] Need Antibody

Jeff,

We do have the Bartonella Henselea antibody listed under Cat Scratch if you 
wanted to pass that info on to Sheila. It is now IVD as well.

http://biocare.net/product/cat-scratch-antibody/

Best regards,

Dax Arguello
Account Executive
Biocare Medical, LLC
Mobile: (925) 586-2591
Fax: (925) 603-8080
E-mail: dargue...@biocare.net

www.biocare.net ٠4040 Pike Lane٠Concord, CA 94520٠(800) 799-9499



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jeffrey Robinson
Sent: Thursday, April 02, 2015 11:40 AM
To: Sheila Fonner; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Need Antibody

Hi Sheila-  I received some information from BioCare last year that they were 
releasing a Bartonella (ASR) antibody.  I just checked their website and I did 
not see it listed but you might want to check with them (www.biocare.net).
Jeff Robinson, Senior Histotechnologist, Sierra Pathology Lab, Clovis, CA.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sheila Fonner
Sent: Thursday, April 02, 2015 10:48 AM
To: Histonet
Subject: [Histonet] Need Antibody

Good afternoon Histonetters!
Does anyone out there know of a good antibody for Bartonella? I would 
appreciate any information you could give me. Thank you for your knowledge and 
willingness to share information.
SheilaKnoxville, TN
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


This email and attachments may contain PHI that is privileged and confidential 
and is not intended for any unauthorized person. If you, the reader, are not 
the intended recipient you are hereby notified that any dissemination, 
distribution or copying of this communication is strictly prohibited. Do not 
read the email but instead reply to the sender and destroy the message and any 
attachments. Thank you.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

RE: [Histonet] Need Antibody

[Jeffrey Robinson]

Hi Sheila-  I received some information from BioCare last year that they were 
releasing a Bartonella (ASR) antibody.  I just checked their website and I did 
not see it listed but you might want to check with them (www.biocare.net).
Jeff Robinson, Senior Histotechnologist, Sierra Pathology Lab, Clovis, CA.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sheila Fonner
Sent: Thursday, April 02, 2015 10:48 AM
To: Histonet
Subject: [Histonet] Need Antibody

Good afternoon Histonetters!
Does anyone out there know of a good antibody for Bartonella? I would 
appreciate any information you could give me. Thank you for your knowledge and 
willingness to share information.
SheilaKnoxville, TN
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


This email and attachments may contain PHI that is privileged and confidential 
and is not intended for any unauthorized person. If you, the reader, are not 
the intended recipient you are hereby notified that any dissemination, 
distribution or copying of this communication is strictly prohibited. Do not 
read the email but instead reply to the sender and destroy the message and any 
attachments. Thank you.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

RE: [Histonet] Embedding Question

[Jeffrey Robinson]

This certainly does not sound ideal to be moving tissue blocks around the 
building.  If this situation cannot be changed then perhaps a plastic bin with 
a snap on lid could help minimize the risk of losing blocks during transport.

Jeff Robinson, Senior Histotechnologist, Sierra Pathology Lab, Clovis, CA.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Paula Sicurello
Sent: Thursday, March 12, 2015 9:47 AM
To: Hannen, Valerie
Cc: HistoNet
Subject: Re: [Histonet] Embedding Question

Why the change? Space. There is a larger space available for histology.

It would be optimum to either move the processors into the new space or leave 
the embedding centers where they are now.

I mentioned did bring up about the samples/wax freezing in transit and the 
reply was the experts didn't mention anything about that

The experts may not have been experienced histotechs.

Paula

On Thu, Mar 12, 2015 at 7:56 AM, Hannen, Valerie  
valerie.han...@parrishmed.com wrote:

 I do not think it to be a good idea.

   1) What is the possibility of a trip and fall in transit?
 Cassettes flying everywhere, possibility of losing any?

   2) Dripping paraffin on a hallway floor-- a slip and fall scenario
 for other people using the much used hallway.

   3) As Tom stated.. not a lean system/process.

 Just my two cents!


 Valerie Hannen,MLT(ASCP),HTL,SU (FL)
 Section Chief, Histology
 Parrish Medical Center
 951 N. Washington Ave.
 Titusville,Florida 32796
 T: (321)268-6333 ext. 7506
 F: (321) 268-6149
 valerie.han...@parrishmed.com
 www.parrishmed.com



 -Original Message-
 From: histonet-boun...@lists.utsouthwestern.edu [mailto:
 histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Podawiltz,
 Thomas
 Sent: Thursday, March 12, 2015 10:32 AM
 To: Paula Sicurello; HistoNet
 Subject: RE: [Histonet] Embedding Question

 Not what I call a lean system. Why the change?

 Tom


 Tom Podawiltz HT (ASCP)
 AP  Section Head
 LRGHealthcare
 603-524-3211 ext: 3220




 -Original Message-
 From: histonet-boun...@lists.utsouthwestern.edu [mailto:
 histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Paula
 Sicurello
 Sent: Thursday, March 12, 2015 9:29 AM
 To: HistoNet
 Subject: [Histonet] Embedding Question

 It has been proposed to move the embedding centers to a room about 210
 ft away from the tissue processors.

 The trip from processor to embedding center would take over 2 minutes
 and require the histotechs to carry the baskets full of cassettes down
 a much used hallway.

 Opinions?

 Do you feel this is a good idea-yes or no and why?

 Thanks in advance,

 Paula
 ___
 Histonet mailing list
 Histonet@lists.utsouthwestern.edu
 http://lists.utsouthwestern.edu/mailman/listinfo/histonet

 THIS MESSAGE IS CONFIDENTIAL.
 This e-mail message and any attachments are proprietary and
 confidential information intended only for the use of the recipient(s)
 named above. If you are not the intended recipient, you may not
 print,distribute, or copy this message or any attachments.  If you
 have received this communication in error, please notify the sender by
 return e-mail and delete this message and any attachments from your
 computer. Any views or opinions expressed are solely those of the
 author and do not necessarily represent those of LRGHealthcare.

 ==
 This email is intended solely for the use of the individual to whom
 it is addressed and may contain information that is privileged,
 confidential or otherwise exempt from disclosure under applicable law.
 If the reader of this email is not the intended recipient or the
 employee or agent responsible for delivering the message to the
 intended recipient, you are hereby notified that any dissemination,
 distribution, or copying of this communication is strictly prohibited.
 If you have received this communication in error, please immediately
 delete this message. Thank you
 ==

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


This email and attachments may contain PHI that is privileged and confidential 
and is not intended for any unauthorized person. If you, the reader, are not 
the intended recipient you are hereby notified that any dissemination, 
distribution or copying of this communication is strictly prohibited. Do not 
read the email but instead reply to the sender and destroy the message and any 
attachments. Thank you.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Mushrooms for GMS fungus control

[Jeffrey Robinson]

How about mushrooms?  Has anyone had any success using mushrooms as a GMS 
fungus control?

Jeff Robinson, Senior Histotechnologist, Sierra Pathology Lab, Clovis, CA


This email and attachments may contain PHI that is privileged and confidential 
and is not intended for any unauthorized person. If you, the reader, are not 
the intended recipient you are hereby notified that any dissemination, 
distribution or copying of this communication is strictly prohibited. Do not 
read the email but instead reply to the sender and destroy the message and any 
attachments. Thank you.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

RE: [Histonet] Controls:

[Jeffrey Robinson]

We have successfully used hamburger meat to make Gram controls.  As far as GMS 
controls go, we ran across a post from someone smearing cream cheese onto lung 
tissue and letting it sit for a couple of days, fixed and processed it and were 
able to demonstrate Aspergillus by GMS.  My fungus control stocks are low so I 
was actually planning to try this with some beef lung.  I haven't heard of the 
Slim Jim method before.

Jeff Robinson, Senior Histotechnologist, Sierra Pathology Lab, Clovis, CA.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jb
Sent: Wednesday, March 04, 2015 1:05 PM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] Controls:

Off the wall question, I have been told that slim jims (pepperoni stick) at the 
gas station can be processed and used as good gram controls. Has anyone done 
this and do they work for GMS also?

Thank you,

Sent from my iPhone
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


This email and attachments may contain PHI that is privileged and confidential 
and is not intended for any unauthorized person. If you, the reader, are not 
the intended recipient you are hereby notified that any dissemination, 
distribution or copying of this communication is strictly prohibited. Do not 
read the email but instead reply to the sender and destroy the message and any 
attachments. Thank you.

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] RE: Ventana Ultra CD10

[Jeffrey Robinson]

Hi Beth Ann-  I have also had problems with the Ventana CD10 after years of 
using it successfully.  I don't know if they changed something or what but it 
just dropped off all of a sudden.  I worked with one of their applications 
specialists on it for some time (different lot #'s, protocol changes, etc.) but 
was still unsuccessful in getting the problem resolved.  I also have a Leica 
Bond on which I also run CD10.  I ended up buying the Leica CD10 and putting it 
in a prep kit on the Ultra.  I have to use the amplifier with it and the 
protocol is a little long (3 1/2 hours I think) but it works OK.  I also have a 
Ventana Benchmark XT but I was unsuccessful in getting the Leica CD10 optimized 
on that instrument (I use the iView detection kit on that so I think that is 
the main problem).  I had a similar problem with the Ventana CD30 and had to 
switch to the CellMarque CD30 in that case but I was able to get that one 
optimized and validated on both the Ultra and the XT.

Jeff Robinson, Senior Histotechnologist, Sierra Pathology Lab, Clovis, CA.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of O'neil, Beth
Sent: Thursday, February 26, 2015 1:22 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Ventana Ultra CD10

We are in the last stages of contract negotiations for purchasing two Ventana 
Benchmark Ultras.  During this time period we have been optimizing our current 
inventory of approximately 110 antibodies and validating before our current 
instrumentation is removed within the next two weeks.  My Ventana application 
specialist is unable to successfully optimize CD10 (Ventana clone SP67) on the 
Ventana Benchmark Ultra.  He had me send slides to their 
applications/troubleshooting lab but they told us they won't start working on 
it until next week and then it would take about a week for them to try and 
optimize it.  We are in an urgent rush to get this antibody optimized and 
validated within the next two weeks since it is heavily requested by our 
Hemepaths.  Would anyone be willing to share their Ultra protocols with me?  
Has anyone had similar experiences with Ventana being unsuccessful and having 
to send their slides to their applications lab for work up?  Thank you for your 
help.

Beth Ann O'Neil, MT(ASCP)SC, HTL, QIHC
one...@wvuhealthcare.commailto:one...@wvuhealthcare.com
Histology Supervisor, Technical Specialist
Lab:  304 - 293 - 6014
Office:  304 - 293 - 7629



-

Confidentiality Notice: This e-mail message, including any attachments, is for 
the sole use of the intended recipient(s) and may contain confidential and 
privileged information.  Any unauthorized review, use, disclosure or 
distribution is prohibited.  If you are not the intended recipient, please 
contact the sender by reply e-mail and destroy all copies of the original 
message.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


This email and attachments may contain PHI that is privileged and confidential 
and is not intended for any unauthorized person. If you, the reader, are not 
the intended recipient you are hereby notified that any dissemination, 
distribution or copying of this communication is strictly prohibited. Do not 
read the email but instead reply to the sender and destroy the message and any 
attachments. Thank you.

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] RE: sections not sticking to charged slides

[Jeffrey Robinson]

HI Renee-  I had a major problem in the past with tissue staining for IHC 
lifting off the slides no matter what I tried.  It turned out that when we 
precut our controls and put them in the oven and then used them later for the 
patient tissue that the charge on the slide was altered.  We currently still 
precut controls on control slides (we use Leica APEX) and then let them just 
air dry with no heat.  We then use those slides when needed and add the patient 
tissue to the bottom and then put the slides in the oven (we use 70C for one 
hour) prior to IHC staining.  The tissue lifting decreased dramatically- even 
on breast tissue.  Be sure to pick up your controls from the wrong (label) end 
when picking up your controls and do not let the bottom half of the slide get 
into the waterbath so as to avoid double-dipping which can also cause tissue 
adherence problems.

Jeff Robinson, Senior Histotechnologist, Sierra Pathology Lab, Clovis, CA.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Renee H. Workman
Sent: Tuesday, February 24, 2015 1:33 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] sections not sticking to charged slides


Help, sections not sticking to charged slides.  We use Mercedes Medical charged 
slides.  Lately have been using sta-on but still have occasional problems 
especially during antigen retrieval.  I need any suggestions.

Renee H. Workman
Histology Supervisor
Virginia Urology
9105 Stony Point Drive
Richmond, VA  23235
W: 804-527-1316 | F: 804-270-0917
rhwork...@uro.com | www.uro.com





Disclaimer: The email and files transmitted with it are confidential and are 
intended solely for the use of the individual or entity to whom they are 
addressed. If you are not the original recipient or the person responsible for 
the delivering the email to the intended recipient, be advised that you have 
received this email in error, and that any use, dissemination, forwarding, 
printing or copying of this email is strictly prohibited. If you received this 
email in error, please delete it from your system without copying it, and 
notify the sender by reply email so that our address record can be corrected.


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


This email and attachments may contain PHI that is privileged and confidential 
and is not intended for any unauthorized person. If you, the reader, are not 
the intended recipient you are hereby notified that any dissemination, 
distribution or copying of this communication is strictly prohibited. Do not 
read the email but instead reply to the sender and destroy the message and any 
attachments. Thank you.

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] RE: Slide and Block Combination Transport or Mailer

[Jeffrey Robinson]

Hi James-  I just looked at my exhibit map from Austin and I believe the item 
you seek (a plastic mailer that holds both block and slide) is sold by 
Evergreen Scientific.

Jeff Robinson, Senior Histotechnologist, Sierra Pathology Lab, Clovis, CA.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Vickroy, James
Sent: Thursday, February 19, 2015 7:40 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Slide and Block Combination Transport or Mailer


I have seen somewhere a combination transport or mailer for both a paraffin 
block and slides.   We are going to have our special stains and IHC stains done 
across town and we use a courier to take the paraffin block to the lab that 
will do the procedure and then they send back the block and the slide(s).   Any 
ideas which vendor carries something like this?   I want to have something in 
which the block and returned slides will be in the same container.   Experience 
tells me things find a way to get separated and lost.

Thanks

Jim

Jim Vickroy
Histology Manager
Springfield Clinic, Main Campus, East Building
1025 South 6th Street
Springfield, Illinois  62703
Office:  217-528-7541, Ext. 15121
Email:  jvick...@springfieldclinic.commailto:jvick...@springfieldclinic.com


This electronic message contains information from Springfield Clinic, LLP that 
may be confidential, privileged, and/or sensitive. This information is intended 
for the use of the individual(s) or entity(ies) named above. If you are not the 
intended recipient, be aware that disclosure, copying, distribution, or action 
taken on the contents of this information is strictly prohibited. If you have 
received this electronic message in error, please notify the sender 
immediately, by electronic mail, so that arrangements may be made for the 
retrieval of this electronic message. Thank you.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


This email and attachments may contain PHI that is privileged and confidential 
and is not intended for any unauthorized person. If you, the reader, are not 
the intended recipient you are hereby notified that any dissemination, 
distribution or copying of this communication is strictly prohibited. Do not 
read the email but instead reply to the sender and destroy the message and any 
attachments. Thank you.

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] RE: processing problem

[Jeffrey Robinson]

Hi Martha-  I have had some limited success in rehabilitating underprocessed 
tissue without reprocessing it in the tissue processor.  If the tissue samples 
are not too large you may be able to let them air dry for a period of time so 
that they dry out.  Make sure to face the blocks if you haven't already done 
so.  You can then melt them down and put them in a mold with liquid paraffin 
and just let them sit on the embeddeder (still in liquid paraffin) for a couple 
of hours for better infiltration.  Re-embed your samples and hope for the best. 
 I hope this helps.

Jeff Robinson, Senior Histotechnologist, Sierra Pathology Lab, Clovis, CA.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Davenport, 
Martha R
Sent: Thursday, February 12, 2015 5:41 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] processing problem

I am Martha Davenport, supervisor, at the University of KY histology lab. We 
had a processing problem caused  by  accidently placing 70% (instead of 100%) 
into the last dehydration container. Would anyone please give me info on how 
you would remedy this?  We usually reprocess the tissue but have had trouble in 
the past with the tissue morphology being optimal. Any help will be greatly 
appreciated.
Martha Davenport 859-257-1822
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


This email and attachments may contain PHI that is privileged and confidential 
and is not intended for any unauthorized person. If you, the reader, are not 
the intended recipient you are hereby notified that any dissemination, 
distribution or copying of this communication is strictly prohibited. Do not 
read the email but instead reply to the sender and destroy the message and any 
attachments. Thank you.

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] RE: Retic Kit Problems

[Jeffrey Robinson]

Yes, Blake, the Benchmark Special Stainer does a very poor job on retics, 
especially bone marrow retics.  We got one in December, 2013, and we do not use 
it for retics (I still have an old Nexes).  I had a Ventana guy here multiple 
times as they thought it was a contamination problem.  He decontaminated it 
several times and they changed some interior filters which helped some.  The 
problem is the heat (or lack of), not the kit.  The old Nexes would heat the 
entire chamber with great results.  The new one has the individual heating pads 
but the current protocol does not give it enough heat for the retic.  Ventana 
says a software upgrade is coming out in the second quarter of this year that 
will hopefully fix the problem.  Keep your fingers crossed!

Jeff Robinson, Senior Histotechnologist, Sierra Pathology Lab, Clovis, CA.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Blake Taylor
Sent: Thursday, February 12, 2015 10:06 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Retic Kit Problems

Anyone using the Ventana benchmark special stainers encountering inconsistent 
staining on your Retics? Same Run one will look great the other not staining 
properly.  Re run it and no issues.  We are shaking the kit well before each 
use and have increased how often we decon.  Any thoughts?

Blake Taylor
Surgical Pathology Supervisor
Lexington Medical Center
803-936-8214
bcdu...@lexhealth.orgmailto:bcdu...@lexhealth.org

PRIVILEGED AND CONFIDENTIAL: This electronic message and any attachments are 
confidential property of the sender. The information is intended only for the 
use of the person to whom it was addressed. Any other interception, copying, 
accessing, or disclosure of this message is prohibited. The sender takes no 
responsibility for any unauthorized reliance on this message. If you have 
received this message in error, please immediately notify the sender and purge 
the message you received. Do not forward this message without permission.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


This email and attachments may contain PHI that is privileged and confidential 
and is not intended for any unauthorized person. If you, the reader, are not 
the intended recipient you are hereby notified that any dissemination, 
distribution or copying of this communication is strictly prohibited. Do not 
read the email but instead reply to the sender and destroy the message and any 
attachments. Thank you.

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Leica Multistainer use for routine Giemsas

[Jeffrey Robinson]

Good Morning-  I have a Leica Multistainer that I currently use only as an 
overflow H  E stainer.  I had high hopes when we purchased the instrument that 
it would prove to be very useful in automating some of our Special Stains.  It 
ended up just being easier to continue running those stains  manually.  I now 
have a request to automate (on the Multistainer) the routine Giemsas that we 
run for H. pylori screening (we use DiffQuik reagents).  Has anyone been 
successful in running Giemsas on this stainer?  My main concern is the slow 
arm speed and I wonder if it will be fast enough to achieve proper 
differentiation when running the slides down through the alcohols.  Any 
feedback would be greatly appreciated!

Jeff Robinson HT, HTL, Senior Histotechnologist, Sierra Pathology Lab, Clovis, 
CA.


This email and attachments may contain PHI that is privileged and confidential 
and is not intended for any unauthorized person. If you, the reader, are not 
the intended recipient you are hereby notified that any dissemination, 
distribution or copying of this communication is strictly prohibited. Do not 
read the email but instead reply to the sender and destroy the message and any 
attachments. Thank you.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

RE: [Histonet] Frozen Sections

[Jeffrey Robinson]

I worked in a hospital Histology Department years ago and the histotechs did 
everything except orient the specimen and read the slide.  The group I work for 
now perform all of their own frozens.  I still cut ORO controls on the cryostat 
but that's about it.  I do have some concerns that the younger histo staff 
members have ZERO experience with frozens.  It's a problem if a pathologist 
does ask for some assistance when there is an issue (especially with 
troubleshooting a cryostat).

Jeff Robinson, Senior Histotechnologist, Sierra Pathology Lab, Clovis, CA.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Hannen, Valerie
Sent: Monday, February 09, 2015 7:03 AM
To: 'wanda.sm...@hcahealthcare.com'; lsmal...@juno.com; 
histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Frozen Sections


As long as there is a Histotech on duty we cut, stain and coverslip all  the 
frozens, after hours the Pathologists do their own.


Valerie Hannen,MLT(ASCP),HTL,SU (FL)
Section Chief, Histology
Parrish Medical Center
951 N. Washington Ave.
Titusville,Florida 32796
T: (321)268-6333 ext. 7506
F: (321) 268-6149
valerie.han...@parrishmed.com
www.parrishmed.com



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of 
wanda.sm...@hcahealthcare.com
Sent: Monday, February 09, 2015 9:21 AM
To: lsmal...@juno.com; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Frozen Sections

We have 4 Pathologist and all four cut their own FS's.  Two will stain and 
coverslip them themselves and two request assistance with staining and 
coverslipping.  We label and set-up the cassette and slides.
Wanda

WANDA G. SMITH, HTL(ASCP)HT
Pathology Supervisor
TRIDENT MEDICAL CENTER
9330 Medical Plaza Drive
Charleston, SC  29406
843-847-4586
843-847-4296 fax

This email and any files transmitted with it may contain PRIVILEGED or 
CONFIDENTIAL information and may be read or used only by the intended 
recipient. If you are not the intended recipient of the email or any of its 
attachments, please be advised that you have received this email in error and 
that any use, dissemination, distribution, forwarding, printing, or copying of 
this email or any attached files is strictly prohibited. If you have received 
this email in error, please immediately purge it and all attachments and notify 
the sender by reply email or contact the sender at the number listed.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of 
lsmal...@juno.com
Sent: Friday, February 06, 2015 10:37 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Frozen Sections

Hi, I need to ask Histoland a questionHow many HT departments provide 
assistance to the pathologist in the performance of frozen sections (cutting 
and staining of slides) to be evaluated by the pathologist?   Thank you very 
much in advance!
Lorraine


Fast, Secure, NetZero 4G Mobile Broadband. Try it.
http://www.netzero.net/?refcd=NZINTISP0512T4GOUT2

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

=
This email is intended solely for the use of the individual to whom it is 
addressed and may contain information that is privileged, confidential or 
otherwise exempt from disclosure under applicable law. If the reader of this 
email is not the intended recipient or the employee or agent responsible for 
delivering the message to the intended recipient, you are hereby notified that 
any dissemination, distribution, or copying of this communication is strictly 
prohibited. If you have received this communication in error, please 
immediately delete this message. Thank you
=

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


This email and attachments may contain PHI that is privileged and confidential 
and is not intended for any unauthorized person. If you, the reader, are not 
the intended recipient you are hereby notified that any dissemination, 
distribution or copying of this communication is strictly prohibited. Do not 
read the email but instead reply to the sender and destroy the message and any 
attachments. Thank you.

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

RE: [Histonet] Problems with Universal Cassette Clamp

[Jeffrey Robinson]

Yes, unfortunately I am very familiar with this problem.  We have probably had 
at least 10 clamp handles break off over the last 3 years.  The problem is the 
soft metal they are using for the clamp post.  The post fits into a hole in the 
bottom portion of the clamp and that is actually what pulls the clamp open so 
that you can insert a cassette.  I have talked to our Leica rep numerous times 
about this issue.  They first tried to blame our techs for the problem (they 
must be using it too aggressively) but they eventually took a limited amount 
of ownership for the inferior materials.  At one point they thickened the 
clamp handle itself but the real problem is that post at the end of the handle. 
 I have suggested that they make the handle (or at least the post) out of 
titanium or some other material that can stand up to the stress put on it by 
the clamp.  I have not seen any additional improvements over the past two years 
and we still have clamp handles break off on occasion.  Your Leica rep is well 
aware of this problem so contact them and ask for some new clamps (they will 
hopefully offer a free one or discounted one).  I try to keep a couple of new 
spare clamps on hand as I have yet to figure out a way to repair that handle 
once it breaks off.

Jeff Robinson, Senior Histotechnologist, Sierra Pathology Lab, Clovis, CA.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Anna Rorick
Sent: Tuesday, February 03, 2015 9:51 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Problems with Universal Cassette Clamp

Hi Everyone,
I am using a Leica RM2255 microtome with a universal cassette clamp. Two of the 
clamps have broken over the past year.
Has anyone else had a problem with these?


Anna Rorick
Anatomic Pathology Technician
Battelle Memorial Institute
Columbus, Ohio
Sent from my iPhone
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


This email and attachments may contain PHI that is privileged and confidential 
and is not intended for any unauthorized person. If you, the reader, are not 
the intended recipient you are hereby notified that any dissemination, 
distribution or copying of this communication is strictly prohibited. Do not 
read the email but instead reply to the sender and destroy the message and any 
attachments. Thank you.

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Amyloid by Congo Red

[Jeffrey Robinson]

Greetings to all Histotechs-  Here's an amyloid question for the braintrust.  
We are cutting our slides and controls at 9 and staining in Congo Red for 1 
hour.  The control stains fine but the patient tissue is staining negative even 
on cases that the pathologist assures us should be positive for amyloid.  We 
are using the Leica APEX charged slides with control and patient tissue on the 
same slide.  Does anyone have any thoughts on why the patient tissue is not 
staining?  Thanks!

Jeff Robinson HT, HTL, Senior Histotechnologist, Sierra Pathology Lab, Clovis, 
CA.


This email and attachments may contain PHI that is privileged and confidential 
and is not intended for any unauthorized person. If you, the reader, are not 
the intended recipient you are hereby notified that any dissemination, 
distribution or copying of this communication is strictly prohibited. Do not 
read the email but instead reply to the sender and destroy the message and any 
attachments. Thank you.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] RE: Problem with cracked paraffin blocks

[Jeffrey Robinson]

Regarding the paraffin/plastic stratification:  we used to get the reddish 
paraffin dust bunnies in the paraffin pots apparently from the separation and 
stratification of the paraffin and the plasticizers.  I bought a big wood 
paddle from the industrial kitchen supply store (about $12) and we stir the 
melted paraffin in the paraffin pots once a day to help keep it mixed up.  It 
seems to have helped some.

Jeff Robinson, HT, HTL, Senior Histotechnologist, Sierra Pathology Lab, Clovis, 
CA.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Wheelock, 
Timothy R.
Sent: Friday, January 23, 2015 9:55 AM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] Problem with cracked paraffin blocks

Hi again  everyone:

I want to thank you all for your advice.
The consensus is that I have my new embedding center's cold plate set way too 
low at -14C, and that I should try raising it to around -5C to cure my cracked 
block problem.
I will run some test blocks over the weekend, and then embed them at this new 
temperature on Monday.
Otherwise my Valida embedding center is working very nicely (as did the 
HistoStar when I demoed it).
The cassette holding tank can accommodate a large number of brain specimens  
and the controls are very easy to use.
Thanks again for your advice.
Have a great weekend.

Tim

Tim Wheelock
Harvard Brain Bank
McLean Hospital
Belmont, MA


The information in this e-mail is intended only for the person to whom it is 
addressed. If you believe this e-mail was sent to you in error and the e-mail 
contains patient information, please contact the Partners Compliance HelpLine 
at http://www.partners.org/complianceline . If the e-mail was sent to you in 
error but does not contain patient information, please contact the sender and 
properly dispose of the e-mail.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


This email and attachments may contain PHI that is privileged and confidential 
and is not intended for any unauthorized person. If you, the reader, are not 
the intended recipient you are hereby notified that any dissemination, 
distribution or copying of this communication is strictly prohibited. Do not 
read the email but instead reply to the sender and destroy the message and any 
attachments. Thank you.

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

RE: [Histonet] HTL exam

[Jeffrey Robinson]

I used the NSH booklets on the various Histology subjects.  I don't know about 
their current availability- I think they were on a CD now but I haven't checked 
lately.  I learned a lot from the booklets as they not only give the correct 
answer they also described why the other answers were wrong along with some 
background pertaining to related subjects.  With the test being online now I 
don't know how important the color plates of the various special stains are but 
I found it extremely helpful to know all of the stains by sight backwards and 
forwards- even stains that we did not run in our lab as there were a lot of 
questions that would refer to different methods of staining for the same 
structure or organism, etc.  I used Sheehan and Bancroft as my texts.  Bancroft 
is British so there is a different slant to his writing that I find 
interesting.  I have read Carson's but I do not feel it has enough background 
information.  Lee Luna's last book has great color plates but the organization 
is poor and it can be hard to find things- I think someone finished it up after 
he passed away.

Jeff Robinson HT, HTL, Senior Histotechnologist, Sierra Pathology Lab, Clovis, 
CA.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Maryann 
Morissette
Sent: Friday, January 23, 2015 10:43 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] HTL exam


Hi all. Was just wondering if anyone has just taken the HTL exam. I passed the 
HT with just reading an older Frieda Carson book.  Can someone give me some 
advice on books that really helped them? Thanks!
Sent from my iPhone

 On Jan 23, 2015, at 1:01 PM, histonet-requ...@lists.utsouthwestern.edu wrote:
 
 Send Histonet mailing list submissions to
histonet@lists.utsouthwestern.edu
 
 To subscribe or unsubscribe via the World Wide Web, visit
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
 or, via email, send a message with subject or body 'help' to
histonet-requ...@lists.utsouthwestern.edu
 
 You can reach the person managing the list at
histonet-ow...@lists.utsouthwestern.edu
 
 When replying, please edit your Subject line so it is more specific 
 than Re: Contents of Histonet digest...
 
 
 Today's Topics:
 
   1. RE: rodent eye (Gowan,Christie C)
   2. Cracking paraffin blocks (Wheelock, Timothy R.)
   3. RE: Cheap Disposable Blades for Facing In (Bea DeBrosse-Serra)
   4. Re: Cracking paraffin blocks (Hans B Snyder)
   5. Amyloid by Congo Red (Jeffrey Robinson)
   6. Thermo  IHC (Cheri Miller)
   7. Problem  with cracked paraffin blocks (Wheelock, Timothy R.)
   8. Re: Amyloid by Congo Red (Michael Ann Jones)
 
 
 --
 
 Message: 1
 Date: Wed, 21 Jan 2015 16:23:06 +
 From: Gowan,Christie C christiecgo...@dermatology.med.ufl.edu
 Subject: RE: [Histonet] rodent eye
 To: Casie Phillips casie4...@gmail.com,
histonet@lists.utsouthwestern.edu
histonet@lists.utsouthwestern.edu
 Message-ID:
ccc0568455733548a03568ac55691f76259...@ahc-mb02.ad.ufl.edu
 Content-Type: text/plain; charset=us-ascii
 
 Hi Casie,
 Hope by now you have rec'd some good tips on rodent eye prep. The only thing 
 I have to offer is that we always used Davidson's fixative for 24 hours and 
 then transferred to 70% ETOH. This worked beautifully preserving all eye 
 components. Good luck and don't forget to check the Histonet archives where I 
 know rodent eyes have been discussed in the past.
 
 Christie Gowan HT (ASCP)
 
 Department of Dermatology
 4037 NW 86th Terrace, 4th Fl
 Mohs Laboratory
 Gainesville, FL 32606
 Phone: 352 594-1529
 
 
 
 From: histonet-boun...@lists.utsouthwestern.edu 
 [histonet-boun...@lists.utsouthwestern.edu] on behalf of Casie 
 Phillips [casie4...@gmail.com]
 Sent: Thursday, January 15, 2015 2:53 PM
 To: histonet@lists.utsouthwestern.edu
 Subject: [Histonet] rodent eye
 
 Good afternoon,
 
 I am currently working with Lewis rats performing corneal alkali 
 injuries at varying strengths. Is there someone there that has prior 
 experience working with a rat eye and would be willing to share 
 information on the most effective ways to preserve, fix and cut the cornea 
 sample.
 
 We are interested in using the cornea without using the whole globe if 
 possible. For now we will be using basic HE staining with a 
 possibility of immunohistochemistry at a later time. The main outcome 
 we are looking for is to find the presence of neutrophils in the 
 cornea. A second objective is to look for any damaged or newly reconstructed 
 tissue.
 
 I would greatly appreciate any advice relating to the type of paraffin 
 used, the ideal length of time to save the tissue and any assistance 
 you can suggest for completing this process  successfully.
 
 Thank you for your time. Any assistance will be greatly appreciated.
 
 Sincerely,
 
 Casie

RE: [Histonet] Re: Embedding

[Jeffrey Robinson]

We use empty pipette tip boxes.  They are the perfect size and fit right under 
the edge and we just toss them when them get full.

Jeff Robinson, HT, HTL, Senior Histotechnologist, Sierra Pathology Lab, Clovis, 
CA.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Boyd, Debbie M
Sent: Thursday, January 22, 2015 10:14 AM
To: gayle.cal...@bresnan.net; 'Tony Auge'; histot...@imagesbyhopper.com
Cc: histonet@lists.utsouthwestern.edu; 'Morken, Timothy'; 'Goins, Tresa'
Subject: RE: [Histonet] Re: Embedding

We use a urine specimen container under the right lower corner of the para 
trimmer and toss it each day.

Debbie M. Boyd HT (ASCP) | Chief Histologist  | Southside Regional Medical 
Center | 200 Medical Park Blvd.  |  Petersburg, Va.  23805 | PH 804-765-5025 | 
FAX 804-765-6058


From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] on behalf of Gayle Callis 
[gayle.cal...@bresnan.net]
Sent: Thursday, January 22, 2015 1:03 PM
To: 'Tony Auge'; histot...@imagesbyhopper.com
Cc: histonet@lists.utsouthwestern.edu; 'Morken, Timothy'; 'Goins, Tresa'
Subject: RE: [Histonet] Re: Embedding

A fabulous idea!   I suspect one could use a cheap travel iron although  one 
needs to devise a way to collect melted paraffin.   Even our fancy para trimmer 
didn't have catch pan for paraffin drippings.I suggest using a new or 
receycled aluminum baking pans available in supermarkets, discount stores or a 
recycled frozen food pan without separations.  These pans come in various sizes 
and depths.   The joy is toss pans when full of paraffin.

Gayle Callis
HTL/HT/MT(ASCP)

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tony Auge
Sent: Thursday, January 22, 2015 9:58 AM
To: histot...@imagesbyhopper.com
Cc: histonet@lists.utsouthwestern.edu; Morken, Timothy; Goins, Tresa
Subject: Re: [Histonet] Re: Embedding

If you want a cheaper alternative you can use a ski wax iron. They cost about 
$40. I mounted one upside down in a bucket and it works just as well as the 
$500 para trimmers.



-Tony Auge HTL (ASCP) QIHC
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

--

Disclaimer: This electronic message may contain information that is 
Proprietary, Confidential, or legally privileged or protected. It is intended 
only for the use of the individual(s) and entity named in the message. If you 
are not an intended recipient of this message, please notify the sender 
immediately and delete the material from your computer. Do not deliver, 
distribute or copy this message and do not disclose its contents or take any 
action in reliance on the information it contains.

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


This email and attachments may contain PHI that is privileged and confidential 
and is not intended for any unauthorized person. If you, the reader, are not 
the intended recipient you are hereby notified that any dissemination, 
distribution or copying of this communication is strictly prohibited. Do not 
read the email but instead reply to the sender and destroy the message and any 
attachments. Thank you.

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] RE: embedding

[Jeffrey Robinson]

I have also noticed a big difference in the brand of cassette being used.  The 
old Tissue-Tek style with the large holes do not seem to bleed or leak much at 
all but the General Data ones we use bleed a lot.  I have tried different 
methods with the General Data type but have yet to come up with a sure fire 
fix.  It could also be affected by the brand of mold-  I think most of ours are 
the Tissue-Tek metal ones so they are probably a better fit for the 
Tissue-Tek cassettes.

Jeff Robinson, HT, HTL, Senior Histotechnologist,
Sierra Pathology Lab, Clovis, CA.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sanders, 
Jeanine (CDC/OID/NCEZID)
Sent: Wednesday, January 21, 2015 8:39 AM
To: Campbell, Tasha M.; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: embedding

Honestly, the easiest thing I think is just don't overfill the base mold. A 
small amount in the bottom, then the tissue, then the cassette top and just top 
off enough paraffin to cover. Unless you accidentally slosh it as you are 
moving it to the cooling tray you will always have clean sides.


Jeanine H. Sanders
Centers for Disease Control and Prevention Infectious Diseases Pathology Branch
404-639-3590
j...@cdc.gov



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Campbell, Tasha 
M.
Sent: Wednesday, January 21, 2015 10:12 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] embedding

Hello everyone, I was just curious if anyone had tips on how to embed without 
getting paraffin on the outside of the cassettes so I don't have to scrape the 
blocks or at least not scrape very much.  Thanks!!









Tasha Campbell, B.S.,HTL(ASCP)

Frederick Gastroenterology Associates

310 W. 9th St.

Frederick, MD 21701

301-695-6800 ext. 144 (w)

304-685-9307 (c)



___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


This email and attachments may contain PHI that is privileged and confidential 
and is not intended for any unauthorized person. If you, the reader, are not 
the intended recipient you are hereby notified that any dissemination, 
distribution or copying of this communication is strictly prohibited. Do not 
read the email but instead reply to the sender and destroy the message and any 
attachments. Thank you.

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] RE: specials stainers

[Jeffrey Robinson]

I have the Leica multistainer.  We use it for H  E staining only.  When we 
attempted to set it up for Special Stains, all of the silver stains had to be 
made up either each run or each day.  Too much hassle.

Jeff Robinson, HT, HTL
Sierra Pathology Lab, Clovis, CA
jrobin...@pathology-associates.com

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K.
Sent: Tuesday, January 20, 2015 6:13 AM
To: 'Mitchell, Janice A'; 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] RE: specials stainers

Oops   -  I had IHC on the brain and didn't pay close attention!

Leica does not have a dedicated special stainer  - but they have a multistainer 
which stains specials as well as HE. Is too slow in a busy lab tho.

I very much recommend the DAKO Artisan..

It is Monday on Tuesday... right?

Joyce Weems
Pathology Manager
678-843-7376 Phone
678-843-7831 Fax
joyce.we...@emoryhealthcare.org



www.saintjosephsatlanta.org
5665 Peachtree Dunwoody Road
Atlanta, GA 30342

This e-mail, including any attachments is the property of Saint Joseph's 
Hospital and is intended for the sole use of the intended recipient(s).  It may 
contain information that is privileged and confidential.  Any unauthorized 
review, use, disclosure, or distribution is prohibited. If you are not the 
intended recipient, please delete this message, and reply to the sender 
regarding the error in a separate email.


-Original Message-
From: Weems, Joyce K.
Sent: Tuesday, January 20, 2015 8:59 AM
To: 'Mitchell, Janice A'; histonet@lists.utsouthwestern.edu
Subject: RE: specials stainers

I recommend Leica.

Joyce Weems
Pathology Manager
678-843-7376 Phone
678-843-7831 Fax
joyce.we...@emoryhealthcare.org



www.saintjosephsatlanta.org
5665 Peachtree Dunwoody Road
Atlanta, GA 30342

This e-mail, including any attachments is the property of Saint Joseph's 
Hospital and is intended for the sole use of the intended recipient(s).  It may 
contain information that is privileged and confidential.  Any unauthorized 
review, use, disclosure, or distribution is prohibited. If you are not the 
intended recipient, please delete this message, and reply to the sender 
regarding the error in a separate email.


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mitchell, 
Janice A
Sent: Tuesday, January 20, 2015 6:43 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] specials stainers

Good Morning,
We are looking for an automatic stainer for special stains.   Ventana vs Dako?  
Thoughts?
Thanks for any input, Janice Mitchell
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



This e-mail message (including any attachments) is for the sole use of the 
intended recipient(s) and may contain confidential and privileged information. 
If the reader of this message is not the intended recipient, you are hereby 
notified that any dissemination, distribution or copying of this message 
(including any attachments) is strictly prohibited.

If you have received this message in error, please contact the sender by reply 
e-mail message and destroy all copies of the original message (including 
attachments).

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


This email and attachments may contain PHI that is privileged and confidential 
and is not intended for any unauthorized person. If you, the reader, are not 
the intended recipient you are hereby notified that any dissemination, 
distribution or copying of this communication is strictly prohibited. Do not 
read the email but instead reply to the sender and destroy the message and any 
attachments. Thank you.

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] RE: Special Stainer

[Jeffrey Robinson]

I must agree with Blake on the BM Special Stainer.  I have had one for over a 
year and have had many problems with it especially trying to run bone marrow 
retics.  Ventana claims a software upgrade will be out in the 2nd quarter so I 
hope to see improvement. Currently, I still use the old Nexes which is 
excellent!

Jeff Robinson, HT, HTL, Senior Histotechnologist
Sierra Pathology Lab, Clovis, CA

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Blake Taylor
Sent: Tuesday, January 20, 2015 10:28 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Special Stainer

We previously had the Ventana Nexus which was excellent, preferred it greatly 
over Dako but now we moved to the Ventana Benchmark Special Stainer and it has 
been nothing but trouble.  I honestly at this point would not recommend the 
Benchmark. It needs improvement.

Blake Taylor
Surgical Pathology Supervisor

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of 
histonet-requ...@lists.utsouthwestern.edu
Sent: Tuesday, January 20, 2015 1:01 PM
To: histonet@lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 134, Issue 23

Send Histonet mailing list submissions to
histonet@lists.utsouthwestern.edu

To subscribe or unsubscribe via the World Wide Web, visit
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
or, via email, send a message with subject or body 'help' to
histonet-requ...@lists.utsouthwestern.edu

You can reach the person managing the list at
histonet-ow...@lists.utsouthwestern.edu

When replying, please edit your Subject line so it is more specific than Re: 
Contents of Histonet digest...


Today's Topics:

   1. RE: Recycling alcohol (Marcum, Pamela A)
   2. Decon HydeAway (Vickroy, James)
   3. RE: specials stainers (Jeffrey Robinson)
   4. Re: Recycling alcohol (Esther C Peters)
   5. FW: Iron control tissue or blocks (Montana, Maria)
   6. Re: Re: Recycling alcohol (Pam Marcum)
   7. RE: specials stainers (Marcum, Pamela A)


--

Message: 1
Date: Tue, 20 Jan 2015 14:22:17 +
From: Marcum, Pamela A pamar...@uams.edu
Subject: [Histonet] RE: Recycling alcohol
To: Horn, Hazel V hor...@archildrens.org, 'Vickroy, James'
jvick...@springfieldclinic.com, histonet@lists.utsouthwestern.edu
histonet@lists.utsouthwestern.edu
Message-ID: 4c96ad5c746b4ba5ba60615893a16...@mail13m2n2.ad.uams.edu
Content-Type: text/plain; charset=us-ascii

Hazel is correct if you use the pure ethanol the paperwork is horrible and the 
limits on what should be in the lab are very low for a normal Histology Lab.  
Reagent alcohol is the best way to go as it has methanol and isopropanol in it 
so it is undrinkable and therefore not under the same ATF rules of usage.  

Pam Marcum
UAMS 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V
Sent: Monday, January 19, 2015 1:05 PM
To: 'Vickroy, James'; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Recycling alcohol

With Ethyl alcohol you will need a license and will have to keep records.  With 
reagent grade alcohol none of that is necessary.

Hazel Horn
Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas 
Children's Hospital
1 Children's Way | Slot 820| Little Rock, AR 72202
501.364.4240 direct | 501.364.1241 fax
hor...@archildrens.org
archildrens.org





-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Vickroy, James
Sent: Monday, January 19, 2015 12:28 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Recycling alcohol

We are planning to recycle alcohol in the new lab I am working with.   
Previously I always used an alcohol blend such as the Flex products.  However 
at this new lab we are going to only process biopsies so I believe I can get by 
using ethanol and not a blend.   We will be getting our alcohol from 
Thermofisher.  Can anyone tell me which alcohols they are using for 
dehydration?  Reagent alcohol or ethyl alcohol.  Obviously we will make our own 
concentrations from a 100%.

Jim

Jim Vickroy
Histology Manager
Springfield Clinic, Main Campus, East Building
1025 South 6th Street
Springfield, Illinois  62703
Office:  217-528-7541, Ext. 15121
Email:  jvick...@springfieldclinic.commailto:jvick...@springfieldclinic.com


This electronic message contains information from Springfield Clinic, LLP that 
may be confidential, privileged, and/or sensitive. This information is intended 
for the use of the individual(s) or entity(ies) named above. If you are not the 
intended recipient, be aware that disclosure, copying, distribution, or action 
taken on the contents

[Histonet] Tissue exchange- Coccidiodes for fungus

[Jeffrey Robinson]

I checked with NSH and they say that their tissue exchange bank has been 
discontinued.  I have a lot of lung tissue containing Coccidiodes organisms 
(Valley Fever).  I would like to trade for some tissue containing fungus.  Is 
there another tissue exchange network out there?  Is anyone interested in 
trading?  Hi Tim.
Jeff Robinson, Senior Histotechnologist, Sierra Pathology Lab, Clovis, CA.


This email and attachments may contain PHI that is privileged and confidential 
and is not intended for any unauthorized person. If you, the reader, are not 
the intended recipient you are hereby notified that any dissemination, 
distribution or copying of this communication is strictly prohibited. Do not 
read the email but instead reply to the sender and destroy the message and any 
attachments. Thank you.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet