Re: [Histonet] reference lab in So. CAL

[Jennifer MacDonald via Histonet]

We were able to find a local lab that experience with the specimens in 
question.  Thank you to all that responded with offers of help.

Thank you, 
Jennifer MacDonald 
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Re: [Histonet] Reference Lab in So. CAL

[Jennifer MacDonald via Histonet]

Thank you to all that responded.  I have a little more information about 
the project. 

The specimens are subdermal implants in mouse.  The specimens are 
cylinders about 5 mm long.  The first student has about 400 specimens and 
there may be more to follow.  The tissue has been fixed in formalin and 
they are currently in the formalin.  The are ready to be processed.  They 
would like H on the tissues.  Turn around time is not critical, but 
they would like it to be done in less than a couple of weeks. 

Local labs are preferred for time and ease of specimen handling reasons. 
They are located in Monrovia.  Shipping out of state or country is not 
feasible. 
Estimated cost and time frame would help.  I can pass your contact 
information on to the facility.

Thank you,
Jennifer MacDonald

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[Histonet] reference lab in So.CAL

[Jennifer MacDonald via Histonet]

I have a colleague looking for a lab that can handle about 400 specimens 
for H staining.  Any recommendations?
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[Histonet] CSH call for abstracts

[Jennifer MacDonald via Histonet]

Hi All,
The California Society for Histotechnology will have its annual symposium 
in Anaheim, CA May 4-6, 2018.  If you are interested in presenting please 
send me your abstract.  Thank you,
Jennifer
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Re: [Histonet] comparison between microtomes leica RM 2125RTS y thermo HM325

[Jennifer MacDonald via Histonet]

The HM325 also has that feature. 



From:   Jay Lundgren via Histonet 
To: Julio Benavides 
Cc: histonet 
Date:   09/01/2017 10:11 AM
Subject:Re: [Histonet] comparison between microtomes leica RM 
2125RTS y thermo HM325



I prefer the Leica, because it has that nifty rough cut button, but that's
just my personal choice.  The Thermo has a better waste tray.  I think
you'd be happy with either one.  The best thing for you to do would be to
get your sales reps to bring them both to your lab to demo and pick your
favorite.

  Jay A. Lundgren, M.S., HTL (ASCP)


On Fri, Sep 1, 2017 at 9:28 AM, Julio Benavides via Histonet <
histonet@lists.utsouthwestern.edu> wrote:

>
> Hi there,
>
> we are buying a new microtome for the lab (research, not high load of
> work) and have been offered leica RM 2125RTS and thermo HM325 with 
similar
> prices.
>
> Could anyone help us to decide?
>
> thanks a lot
>
> cheers
>
> Julio
>
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[Histonet] Digital Microscopes

[Jennifer MacDonald via Histonet]

Is anyone familiar with digital scanning microscopes?  We are in the 
market for one.
Thank you.
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Re: [Histonet] ClearRite

[Jennifer MacDonald via Histonet]

For many years we used ClearRite, both on our tissue processor and for 
staining.  We are now using a similar product from another company.  We 
did not see any problems with our IHC with the use of this product.  We do 
not do molecular testing.  We found it to be far superior to the limonenes 
and much safer to use than xylene.  Coverslipping is another issue.  Not 
all mounting media are compatible with the aliphatic hydrocarbons.  We 
have good success with Permount.
Jennifer MacDonald




From:   "Cartun, Richard via Histonet" 
To: "histonet@lists.utsouthwestern.edu" 

Date:   06/09/2017 10:36 AM
Subject:[Histonet] ClearRite



Anyone using ClearRite (xylene replacement) for tissue processing?  If so, 
what's been your experience?  Any effect on IHC or molecular testing? 
Thank you!

Richard

Richard W. Cartun, MS, PhD
Director, Histology & The Martin M. Berman, MD Immunopathology & 
Morphologic Proteomics Laboratory
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596
(860) 545-2204 Fax


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Re: [Histonet] heated block trimmers

[Jennifer MacDonald via Histonet]

We have two of the Paratrimmers by Shandon.  They really cut down on the 
mess with scraping the blocks and the repetitive motion.



From:   "Underwood, Fred via Histonet" 
To: "histonet@lists.utsouthwestern.edu" 

Date:   05/24/2017 06:04 AM
Subject:[Histonet] heated block trimmers



Hi All,

Anyone have any experience with the heated block trimmers?  Specifically, 
I was looking at the one from Newcomer.

Thanks,
Fred
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[Histonet] California Society for Histotechnology

[Jennifer MacDonald via Histonet]

It's not too late to register for the 2017 CSH 40th Annual symposium. Lots 
of great workshops! 
No late fees!

http://californiahistology.org/events.html#csh2017
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[Histonet] CE opportunities

[Jennifer MacDonald via Histonet]

   The California Society for Histotechnology is hosting their annual
   symposium May 18-21 in San Jose.  Please join us for some great
   workshops and California spring weather.  San Jose is a great airport
   to fly into.

   More information can be found at:
   http://californiahistology.org/events.html
   The deadline for CSH hotel pricing is April 28.
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Re: [Histonet] hirsch-peiffer cresyl violet method?

[Jennifer MacDonald via Histonet]

Tim,
I have this method in one of medical technology books from many years ago. 
 Lynch's Medical Laboratory Technology II.  The author of the Histology 
section was Charles F. A. Culling.  There are two procedures, one for 
urine and one for nervous system tissue.  I assume you want the nervous 
system?

Procedure:
1.  Cut 8 to 12 um frozen sections of formalin-fixed tissue into distilled 
water.
2. Stain free-floating sections in 1% cresyl echt violet in 1% acetic acid 
(pH about 2.7) for 10 minutes (pH 3.5 to 3.6 is preferable) as with urine.
3. Rinse in distilled water and mount in glycerin.

Results
Granules of metachromatic leukodystrophy - golden brown that remains 
uncvhanged after treatment with 1% ammonium water.
The granules stain positively with PAS in paraffin sections, but the 
present author has fuond that the PAS-positive reaction faded in routinely 
mounted sections after 6 to 12 months.

I can scan the page for you if you'd like.
Jennifer



From:   "Morken, Timothy via Histonet" 
To: Histonet 
Date:   04/14/2017 11:45 AM
Subject:[Histonet] hirsch-peiffer cresyl violet  method?



Hi all,

Does anyone have the method for Hirsch-peiffer cresyl violet used  for 
metachromatic leukodystrophy? On frozen sections.

We are trying to find out if it is different than the usual cresyl violet 
with acetic acid method for Nissl bodies.

I've found many references to it, but none that give the procedure. And 
the original paper is from 1955 not easily available. And is in German



Thanks for any help.

Tim Morken
Pathology Site Manager, Parnassus
Supervisor, Electron Microscopy/Neuromuscular Special Studies
Department of Pathology
UC San Francisco Medical Center

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Re: [Histonet] egg albumin

[Jennifer MacDonald via Histonet]

I respectively disagree with this analogy.  Eggnog is dairy based, made 
with milk, heavy cream and sugar.  Dairy products have a much shorter life 
than egg whites.




From:   Rene J Buesa via Histonet 
To: Nancy Schmitt , 
"'histonet@lists.utsouthwestern.edu'" 
Date:   04/12/2017 09:07 AM
Subject:Re: [Histonet] egg albumin



Do you have a bottle of "Eggnog"? Egg-albumin will have similar shelf life 
if refrigerated.René 

On Wednesday, April 12, 2017 7:40 AM, Nancy Schmitt via Histonet 
 wrote:
 

 Good Morning-

Thoughts on shelf life of egg albumin?

Thank you

Nancy Schmitt MLT, HT(ASCP)


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[Histonet] Clinical Laboratory Educators' Conference

[Jennifer MacDonald via Histonet]

This is a great opportunity for those teaching histotechnology.  Although 
many of the workshops are CLS oriented there are many that are about 
teaching laboratory science in general.  There are also workshops about 
clinical rotation issues.
I have gone several years and always bring back great information for our 
program.
Below is a link for the information.


http://www.ascls.org/education-meetings/clec
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[Histonet] California State Histo Symposium

[Jennifer MacDonald via Histonet]

The California Society for Histotechnology will be hosting the 41st Annual 
Symposium in San Jose May 19-21 at the Hilton.  We are accepting abstracts 
for workshops at this time.
Although it is the 41st we will be celebrating our 40th. 

Please email me with your abstract.

jmacdonald...@gmail.com
or
jmacdon...@mtsac.edu
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Re: [Histonet] Congo Red Controls

[Jennifer MacDonald via Histonet]

We cut ours at 8 um within a couple of days of when we are going to use 
them.  We bake them as usual.



From:   "Myles, Irene via Histonet" 
To: "'histonet@lists.utsouthwestern.edu'" 

Date:   11/14/2016 12:26 PM
Subject:[Histonet] Congo Red Controls



Good Afternoon Histonet,

How does everyone handle their Congo Red Controls; thickness, room temp or 
refridgerator, how long do they last once cut, keep them baked or unbaked 
and anything else I did not list.  Thank you very much.

Irene

Irene Myles, HT (ASCP)
Senior Histology Technician
Brigham and Women's Hospital
Histology; Dept. of Pathology
Amory Bldg; 3rd flr; RM 368J
75 Francis Street
Boston, MA 02215
617-732-5454



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Re: [Histonet] Bachelor's degrees to sit for HTL

[Jennifer MacDonald via Histonet]

Fawn,
There is no specification as to what type of degree it must be.  It can be 
any baccalaureate degree as long as you have at least 30 semester hours of 
biology and chemistry combined.  I have had students with art and 
psychology degrees sit for the HTL exam.  They came back to college to get 
the additional science classes to satisfy the HTL (ASCP) requirement.
Jennifer



From:   Fawn Bomar via Histonet 
To: "histonet@lists.utsouthwestern.edu" 

Date:   10/20/2016 08:27 AM
Subject:[Histonet] Bachelor's degrees to sit for HTL



Hi everyone,



I was wondering if anyone would be able to help my co-worker and myself in 
trying to figure out exactly what we need to do to obtain a bachelor's 
degree that would qualify us to sit for the HTL.  As of right now we both 
have associate's degrees and our HT certification.  We both work full time 
and have children so we cannot attend college full time and would have to 
do most of it online if possible due to our location in South Boston, 
Virginia.



We are trying to figure out how to blend online classes with the science 
classes that may have labs required that we would have to attend campus 
for.



Another question we are having is what specific bachelor's degree do we 
have to obtain, does it have to be a biology degree or a science degree?



Thanks for all your help,



Fawn Bomar
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Re: [Histonet] taking the HTL

[Jennifer MacDonald via Histonet]

This may be a better option:  
https://www.labce.com/histology_exam_simulator.aspx
The NSH has partnered with media lab for exams.  You have a full year 
access to the questions.  There are over 2,000 questions in the question 
bank and there are images as well.  NSH members pay the discounted rate of 
$79.  Non NSH members pay $99.

Jennifer



From:   Lauren Sweeney via Histonet 
To: "histonet@lists.utsouthwestern.edu" 

Date:   09/19/2016 10:36 AM
Subject:[Histonet] taking the HTL



Hello all,

I was wondering if anyone out there has used the HTL practice tests 
package you can buy from ASCP to prepare for their exam, and if you did 
was it worth $30?

Thanks!

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[Histonet] Nurses in charge of labs

[Jennifer MacDonald via Histonet]

This decision could affect histology labs as well.  Please read below and 
consider the effects of having a nurse in charge of the lab.
Jennifer

Petition Urging CMS to Reconsider Ruling on Nursing Degree Equivalence

ASCLS, in conjunction with our partners on the Board of Certification, 
urge the laboratory community and other interested individuals to Sign the 
Petition urging the Centers for Medicare and Medicaid Services (CMS) to 
reconsider its position that nursing is a biological science for purposes 
of performing laboratory testing.
[ASCLS_Logo]
Government Affairs Alert


Sign Petition Urging CMS to Reconsider Ruling on Nursing Degree 
Equivalence
ASCLS, in conjunction with our partners on the Board of Certification, 
urge the laboratory community and other interested individuals to Sign the 
Petition urging the Centers for Medicare & Medicaid Services (CMS) to 
reconsider its position that nursing is a biological science for purposes 
of performing laboratory testing.

Sign the petition here.<
http://weblaunch.blifax.com/listener3/redirect?l=8b3397a5-c6e1-4643-a69c-00f119b9c805=ca0c8eaf-ab6f-e611-95c6-0050569f409f=http%3a%2f%2fcqrcengage.com%2fascpath%2fapp%2fsign-petition%3f0%26engagementId%3d239813
>

On April 1, CMS announced that “an associate’s or bachelor’s degree in 
nursing is equivalent to an associate’s or bachelor’s degree, 
respectively, in biological science”—seemingly declaring that individuals 
with a nursing degree are potentially as qualified to perform advanced 
testing as certified laboratory professionals. It also appears that CMS’s 
position could allow individuals with as little as a bachelor’s degree in 
nursing to direct a CLIA moderate complexity laboratory and/or serve in 
senior supervisory roles within a CLIA high complexity laboratory. Since 
the Clinical Laboratory Improvement Amendments (CLIA) of 1988 doesn’t 
specifically require clinical training of individuals with a degree in 
biological sciences, CMS’s new policy exempts individuals with a 
bachelor’s degree in nursing from any specific training requirement prior 
to performing high complexity testing for diagnostic purposes.

We have great respect for the work and invaluable services nurses provide 
patients, but we do not agree that the nursing degree is equivalent to a 
biological sciences degree or that it would adequately prepare someone to 
perform non-waived laboratory services.

Our greatest concern is this policy will negatively affect test quality 
and patient outcomes as well as effect access to quality testing services. 
Take a few minutes to Sign the Petition to tell CMS<
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> that you believe a degree in nursing is not the same thing as a degree 
in the biological sciences and that appropriate academic coursework and 
clinical training/experience are need to provide quality testing services.







If you have any questions, please direct them to ASCLS Executive Vice 
President Jim Flanigan via email at j...@ascls.org.



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Re: [Histonet] Eosin

[Jennifer MacDonald via Histonet]

Elizabeth,
You can try to lower the pH of the eosin a bit.  Add some acetic acid. Use 
absolute alcohol after the eosin for differentiating/dehydrating.
Jennifer



From:   "Cameron, Elizabeth via Histonet" 

To: "histonet@lists.utsouthwestern.edu" 

Date:   08/30/2016 07:29 AM
Subject:[Histonet] Eosin



Hi,
I have been staining fish tissues fixed in Davidsons with H, and the 
researcher would like the eosin to be more intense.  Our standard protocol 
works well for our own tissue, but the fish look much more washed out.  I 
am using alcoholic eosin Y, have tried both water and alcohol before and I 
have varied the alcohol differentiation steps after the Eosin.  I also 
extended the time in Eosin and increased the wash after bluing to make 
sure the sections are not basic.  Any suggestions would be appreciated.
Thank you.

Elizabeth M. Cameron, HT(ASCP), QIHCCM
Lead Histologist
Mid Coast Hospital
123 Medical Center Drive
Brunswick, ME 04011
(207) 373-6573

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Re: [Histonet] On the hunt for new microtomes!

[Jennifer MacDonald via Histonet]

Your ignorant sarcasm isn't appreciated. Leica makes the microtome and
private labels it for Sakura.

Sent from my iPhone

> On Aug 2, 2016, at 7:46 AM, Rene J Buesa via Histonet
 wrote:
>
> As far as I know Leica Microsystems has bought/absorbed along the years
many optical/instruments makers, such as Baush, American Optical,
Cambridge Instruments, Wild-Heerburg (Switzerland, although this one was a
"reverse" acquisition), Australian "Bond" manufacturers, NOVOCASTRA
laboratories BUT I have never heard that it has bought Sakura instruments.
It would be nice if somebody has reliable information about this alleged
acquisition.René
>
>On Monday, August 1, 2016 4:02 PM, Rene J Buesa via Histonet
 wrote:
>
>
> I don't know now, but some years ago Thermo instruments were less that
reliable. Try Leica or even better Sakura.René
>
> On Monday, August 1, 2016 3:20 PM, Mary Faith Encarnacion via
Histonet  wrote:
>
>
> Hey HistoNet,
>
> Thanks to everyone who helped me out by providing their opinions on
> embedding centers.  This time, I need everyone's thoughts on microtomes.
> My lab is debating between the Thermo/Microm HM355S and the Leica RM2255.
> Your thoughts and advice are very much appreciated!  If there are any
more
> I should try, let me know!
>
> Thanks again,
> Mary Faith
> Histology Supervisor
> VA Palo Alto Health Care System
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Re: [Histonet] On the hunt for new microtomes!

[Jennifer MacDonald via Histonet]

Lecia makes the Sakura microtome.  We have both Sakura and Microm 
microtomes in our student lab.  They have both proved to be reliable. 
There is a difference between a Shandon microtome and a Microm microtome. 



From:   Rene J Buesa via Histonet 
To: Mary Faith Encarnacion , 
"histonet@lists.utsouthwestern.edu" 
Date:   08/01/2016 12:42 PM
Subject:Re: [Histonet] On the hunt for new microtomes!



I don't know now, but some years ago Thermo instruments were less that 
reliable. Try Leica or even better Sakura.René 

On Monday, August 1, 2016 3:20 PM, Mary Faith Encarnacion via Histonet 
 wrote:
 

 Hey HistoNet,

Thanks to everyone who helped me out by providing their opinions on
embedding centers.  This time, I need everyone's thoughts on microtomes.
My lab is debating between the Thermo/Microm HM355S and the Leica RM2255.
Your thoughts and advice are very much appreciated!  If there are any more
I should try, let me know!

Thanks again,
Mary Faith
Histology Supervisor
VA Palo Alto Health Care System
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Re: [Histonet] Reticulin Stain

[Jennifer MacDonald via Histonet]

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Re: [Histonet] block scrapers

[Jennifer MacDonald via Histonet]

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Re: [Histonet] Cleaning Tissue Molds

[Jennifer MacDonald via Histonet]

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Re: [Histonet] Poor Staining

[Jennifer MacDonald via Histonet]

Heat can cause poor chromatin staining.  Is the two shades of eosin only 
on the biopsies, or all tissues? 



From:   Charles Riley via Histonet 
To: histonet@lists.utsouthwestern.edu
Date:   06/16/2016 11:19 AM
Subject:[Histonet] Poor Staining



Can over processing small biopsies lead to poor chromatin staining? Also
can this cause an issue with the eosin staining ( for example only getting
to shades of pink instead of three)?

Please give me any feedback you can

-- 

Charles Riley HT(ASCP)CM

Histopathology Coordinator/ Mohs
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Re: [Histonet] warthin-starry stain

[Jennifer MacDonald via Histonet]

are you doing a microwave method or traditional?



From:   Jeff Halstead via Histonet 
To: "histonet@lists.utsouthwestern.edu" 

Date:   06/16/2016 10:17 AM
Subject:[Histonet] warthin-starry stain



Hello histonet---I am a tech in western Oregon, and I have been 
experiencing strange problems with the afore mentioned stain. When the 
stain is run a very strange grey precipitate deposits upon the slides. The 
deposition is not uniform across the slides and varies from slide to slide 
within the run. I use bleach washed slides and all glassware is also 
bleach washed. The slides I am using are non charged with only frosted 
top. The last two runs I used reagents from just opened bottles. No meatal 
contacts the slides or the glassware used to assimilate the reagents. As I 
said---what gives. To say I am perplexed is understating my mood. I have 
even tried washing the slides in the hot water bath to no improvement. My 
path has a list of cases to run and is a bit anxious. Please help. Anyone 
with any ideas ? one step I did not mention-I bleach wash the slides-wash 
in di water-soak in 100% ethanol-then air dry and cover. Also thought I 
might mention I de-wax the pookers from 30 minut
 es to 45 minutes. Thanx for the time   jeff
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Re: [Histonet] Powder Picric Acid vs Liquid Picric Acid

[Jennifer MacDonald via Histonet]

0.5 grams in 400 mL is 0.125%.  Dilute your 1.3% tenfold and it will be 
close enough.



From:   Amy Johnson via Histonet 
To: "histonet@lists.utsouthwestern.edu" 

Date:   06/13/2016 10:47 AM
Subject:[Histonet] Powder Picric Acid vs Liquid Picric Acid



Our current procedure for the Brown and Brenn Gram stain uses 0.5 grams of 
picric acid to 400mls of acetoneis there a way to use a 1.3% 
Picric Acid solution in place of the powdered Picric Acid?

Amylin Johnson, B.S. HTL(ASCP)
Associates in Pathology
Wausau Wi 54401
715-847-2130

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Re: [Histonet] Histonet for Med Techs

[Jennifer MacDonald via Histonet]

clse...@list.apsu.edu
This is a list serve for MT/MLT Educators.

Sent from my iPhone

> On May 23, 2016, at 6:09 AM, Pam Barker via Histonet
 wrote:
>
> Hi Histonetters!
> I hope everyone is looking forward to a great week and a fantastic
Memorial
> Day Weekend.  I am hoping someone can help me with this.
> I would like to know if anyone is aware of a forum similar to histonet
but
> for med techs?  (Besides Medlab out of the University of Buffalo).
>
> Thanks-Pam
>
> Right Place, Right Time, Right Move with RELIA!
>
> Thank You!
>  Pam M. Barker
>
> Pam Barker
> President/Senior Recruiting Specialist-Histology
> RELIA Solutions
> Specialists in Allied Healthcare Recruiting
> 5703 Red Bug Lake Road #330
> Winter Springs, FL 32708-4969
> Phone: (407)657-2027
> Cell: (407)353-5070
> FAX: (407)678-2788
> E-mail: rel...@earthlink.net
> www.facebook.com/PamBarkerRELIA
> www.linkedin.com/in/reliasolutions
> www.twitter.com/pamatrelia
>
>
>
>
>
>
>
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Re: [Histonet] PAS Stain

[Jennifer MacDonald via Histonet]

We switched form malt diastase to alpha amylase and have seen a 
significant improvement in digestion, but as others have said enzyme 
activity will be destroyed at 60 degrees C. We put ours into a 37 degree C 
water bath.  Enzymes work optimally at 37C, body temperature.  As the 
temperature rises above 37C you will see a decrease in enzyme activity and 
then none at all.
Jennifer



From:   Joanne Clark via Histonet 
To: "histonet@lists.utsouthwestern.edu" 

Date:   05/04/2016 01:04 PM
Subject:[Histonet] PAS Stain



Hi Histonetters, I'm hoping someone can help me troubleshoot our PAS 
diastase method.  We have been digesting the tissue in 0.5% diastase of 
malt in a 60 degree oven for 30 minutes, but do not see the glycogen being 
digested out.  I have tried alpha amylase and beta amylase also without 
any luck.  Does anyone have any suggestions to get the digestion to work

Joanne Clark, BAAS, HT(ASCP)CM
Director of Histology

P.   (575) 622-5600
C.   (575) 317-6403
F.   (575) 622-3720
TF. (800) 753-7284

pcnm.com




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Re: [Histonet] Sea urchin Spines

[Jennifer MacDonald via Histonet]

I'm told that it is the ammonia in urine that is the "active ingredient". 
Perhaps trying ammonia water?



From:   "deGuzman, Jose R via Histonet" 

To: "histonet@lists.utsouthwestern.edu" 

Date:   04/27/2016 01:58 PM
Subject:Re: [Histonet] Sea urchin Spines



Have you tried urine or something similar? I know that urine dissolves the 
spine. 



Does anyone have any tips on how to get a section of a sea urchin spine? I 
tried decal but that didn't soften it at all and I tried using nair. If 
anyone has any suggestions please let me know

-- 

Charles Riley HT(ASCP)CM

Histopathology Coordinator/ Mohs



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Re: [Histonet] Blog Post Not lab related

[Jennifer MacDonald via Histonet]

In addition to the Histonet, I am on a listserve for clinical laboratory 
educators.  Dr. Raff posts his blog link on that listserve as well.   He 
also responds to some of the questions.  There has never been one public 
complaint regarding his posts.  It is very clear in the subject line and 
the ability to delete his posts are quite easy.  Please don't give 
credence to the myth that the clinical lab has more patience and common 
sense than the histology world. 



From:   Rene J Buesa via Histonet 
To: Caroline Miller , Lester Raff MD 

Cc: "histonet@lists.utsouthwestern.edu" 

Date:   04/14/2016 06:02 AM
Subject:Re: [Histonet] Blog Post Not lab related



Amen!!!But you have to concede that Lester is a very  persistent, almost 
obstinate individual, probably used to impose his will and this postings 
are just an example of it: he likes his blog and tries to impose it to 
everyone. Evidently he has all the time in the world and just does not 
know what to do with it and enjoys sharing his "witty" side. René 

On Wednesday, April 13, 2016 3:24 PM, Caroline Miller via Histonet 
 wrote:
 

 Lester, I do believe this is the second 'non lab related' blog post this
week. As we have spoken about before I do not think histonet is an
appropriate place for your blog posts. I, personally, was totally OK with
you co-posting with other lab related topics. However you are now pushing
it and I respectfully ask that you refrain from sending out your blog 
posts
to the whole list, let people sign up if they are interested.

The issue is that this is a histology related list, if everyone posted
non-lab stuff here it would soon be chaos!

Respectfully yours,
mills

On Wed, Apr 13, 2016 at 11:38 AM, Lester Raff MD via Histonet <
histonet@lists.utsouthwestern.edu> wrote:

> Just some philosophy towards the end of a long day.
>
>
> 
http://www.chicagonow.com/downsize-maybe/2016/04/kitten-power-can-get-things-done/

>
>
>
> Just a reminder-I try to limit my blog invitations here. If you enjoy 
the
> blog, remember to subscribe (no charge, no spam) on the ChicagoNow blog
> site.
>
> Thanks for your readership.
>
> Lester J. Raff, MD MBA
> UroPartners
> Medical Director Of Laboratory
> 2225 Enterprise Dr. Suite 2511
> Westchester, Il 60154
> Tel: 708-486-0076
> Fax: 708-492-0203
>
> ___
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>



-- 
Caroline Miller (mills)
Director of Histology
3Scan.com
415 2187297
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Re: [Histonet] Reference for erythrosin-eosin counterstain for H

[Jennifer MacDonald via Histonet]

There is a reference to erythrosin in Lynch's Medical Laboratory 
Technology, but it is brief.  Culling is the author of the histology 
chapter, so perhaps he has something more in depth.



From:   "Tony Henwood (SCHN) via Histonet" 

To: "histonet@lists.utsouthwestern.edu" 

Date:   04/03/2016 05:20 PM
Subject:[Histonet] Reference for erythrosin-eosin counterstain for 
H



Hi all,

I am doing an H workshop next month and one of the Hx counterstains I 
will be including is Erythrosin-eosin (see below).
This counterstain is the preferred by some of our major Histopathology 
departments in Australia.

The issue I have is that I cannot find a reference for this variant of the 
H

Anyone have any ideas?


Eosin Y (CI 45380) 5g
Erythrosin B (CI 45430)   5g
Sodium Hydrogen Carbonate 1.25g
Magnesium Sulphate 10g
Distilled water   500ml
Regards
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA)
Principle Scientist, the Children's Hospital at Westmead
Adjunct Fellow, School of Medicine, University of Western Sydney
Tel: 612 9845 3306
Fax: 612 9845 3318
Pathology Department
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA


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Re: [Histonet] Nuclear Bubbling

[Jennifer MacDonald via Histonet]

There is no need to be rude.  He has tried the drying option and is still 
having nuclear bubbling.  He is exploring other possible issues.  You 
would see this if you read the email in its entirety. 



From:   Rene J Buesa via Histonet 
To: "Vickroy, James" , 
"histonet@lists.utsouthwestern.edu" 
Date:   02/16/2016 10:13 AM
Subject:Re: [Histonet] Nuclear Bubbling



If I remember correctly, this issue has been discussed previously.The 
general consensus as to the cause of nuclear "bubbling" (in reality a lack 
of staining in the nuclear area) has been attributed to an incomplete 
section drying.After the section has be "fished" from the water bath, if 
the slide is not set to drain the underneath water before drying, the 
nuclear components are dissolved hence when the section is stained, there 
is nothing to stain → "nuclear bubbling".I think this has been previously 
stated so I really do not understand posting this same question again.I do 
not think that posting again the question a different answer is going to 
be received.rené 

On Tuesday, February 16, 2016 12:32 PM, "Vickroy, James via Histonet" 
 wrote:
 

 
Struggling to find an answer.  We do a lot of GI biopsies in our lab.  
Sometimes they look wonderful without any nuclear bubbling, other times 
the bubbling is pretty intense.  Since nuclear bubbling is often 
attributed to incomplete fixation we of course have investigated the 
fixation times.  I do not find that the problem is fixation.  In fact some 
of the biopsies end up fixing for 48 hrs before processing. (weekend).  
There was a suggestion last week or so that there might be water trapped 
under the slides after cutting and before staining.  I really thought that 
this might be the issue however I'm not sure at this point.  Extra drying 
seems to help but sometimes slides side by side are so variable, one with 
bubbles and one without.  I also don't believe the problem is in the 
processing schedule since the problem has shown up on both a rapid and a 
normal schedule. (therefore longer dehydration, clearing, etc.)

I am wondering if anyone else has worked with this issue.  Here are my 
questions:


1.Could it be something that is happening with the tissue before 
it gets to the lab?  Usually a delay if fixation  causes other artifacts 
but not bubbling.  Could it be heat from the GI procedure?

2.  We do use blue sponges for our biopsies.  I know some say get rid 
of the sponges but has anyone seen this problem caused by usage of 
sponges?

3.  What about the heat stage in our Prisma stainer?


I am really getting frustrated.  Pathologists never complain however I 
would rather all of the tissue did not have the "nuclear bubbling".  Again 
we only do biopsies so I really don't think the standard old " not enough 
time in formalin" is the issue.  I have even wondered about variables such 
as we use recycled formalin, recycled Clearite III.

Any suggestions?

Jim



Jim Vickroy
Histology Manager
Springfield Clinic, Main Campus, East Building
1025 South 6th Street
Springfield, Illinois  62703
Office:  217-528-7541, Ext. 15121
Email:  jvick...@springfieldclinic.com<
mailto:jvick...@springfieldclinic.com>



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Re: [Histonet] unsolved proplem

[Jennifer MacDonald via Histonet]

There are a couple of things that it might be. 
1.   Uneven deparaffinization before staining.
2.  Water in your last reagents/paraffin on the processor.



From:   mohamed abd el razik via Histonet 

To: "Histonet@lists.utsouthwestern.edu" 

Date:   01/26/2016 11:33 AM
Subject:[Histonet] unsolved proplem



Dear allI have submitted a proplem about reprocessing tissue blocks that 
have patches of stained areas and unstained pale yellow areas (H 
stain)!!!, unfortently the proplem didn't solved yet and i have tried all 
possible solutions, increasing processing time and ordered new chemicals 
from other soruces and changed the paraplast company but still have the 
proplem  
i'm using 70% alc 2hr - 80% alc 1hr - 90% alc 45mins- absolut 1 and 2 each 
for 30 mins then Benzen 1 and 2 each for 25 mins then clearing by methyl 
benzoat 2hs at least then paraffin1+2 (Maccormick melting degree 
56-58)each 90 mins for mice kidney, liver and tests
the previous protocol was very satisfactory to us and we have tried to 
stain older sections processed befor the emerging proplem and have a nice 
stain by current H stain to ensure that the proplem is out our staining 
step. 
any suggestions please !!
Mohamed


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Re: [Histonet] Processing of breast tissue

[Jennifer MacDonald via Histonet]

Do you using "freezing spray" on these specimens?  Direct spray can cause 
the tissue to appear burnt. 



From:   Charles Riley via Histonet 
To: histonet@lists.utsouthwestern.edu
Date:   01/05/2016 06:00 AM
Subject:[Histonet] Processing of breast tissue



We have been experiencing some issue lately with cutting our breast
excisions. Recently we had two specimen where after our 8 hr run the 
tissue
was still extremely soft. Our medical director said the fat sectioned
beautifully but the fibrous tissue appeared cooked. Can anyone give a
suggestion as to how we should process our breast excisions from grossing
through to microtomy sectioning.

Currently I have tried to get grossing to limit the specimen to 2mm by 2mm
by 1mm thick. We run an 8hr process using the Thermo Shandon Excelsior
processor. We cut our sections at 4um but have recently had to do them at
6um in order to get the soft samples cut.
-- 

Charles Riley HT(ASCP)CM

Histopathology Coordinator/ Mohs

Doctors Pathology Services, Dover DE
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[Histonet] Leica rep in SoCal

[Jennifer MacDonald via Histonet]

Looking for the contact information for Leica sales in Southern 
California.
Thanks,
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Re: [Histonet] Hourly wage for trainee in CA

[Jennifer MacDonald via Histonet]

Akemi,
I think this offer is low for a certified HT/HTL, particularly in that 
area.  I have had graduates go up to Northern CA and the minimum starting 
wage that they have accepted is $25.  These are brand new graduates, with 
minimal experience, but I don't believe their titles are "trainees".
Jennifer



From:   Eileen Akemi Allison via Histonet 

To: Histonet 
Date:   11/28/2015 08:08 AM
Subject:[Histonet] Hourly wage for trainee in CA



Hello all my histology friends in California: 
1st I hope you all had a wonderful Thanksgiving! I would like to know what 
the hourly rate of a new histologist with a HT certification is making. I 
would very much appreciate this information.  We are offering a training 
position to a local individual who doesn't have a degree, HT or HTL 
certification, or recent experience (last worked in histology in 1991). I 
want to see if our offer of $20.00 an hour plus benefit's was out of line. 
 I would prefer to have a certified HT, but no one has answered our ads.

Best regards,

Akemi Allison BS, HT/HTL (ASCP)
Pathology Manager
Monterey Bay GI Consultants Laboratory
23 Upper Ragsdale Drive, Suite 200
Monterey, CA 93940
W: Email: aalli...@montereygi.com 
H: Email: akemiat3...@gmail.com 
Tele: (831) 375-3577 X117

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Re: [Histonet] IHC hand staining

[Jennifer MacDonald via Histonet]

In one of our classes the students do IHC staining by hand.  They perform 
multiple procedures over the semester.  We currently use Biocare reagents. 
 The polymer based detection systems are simple to use.  The data sheets 
that accompany the antibodies and detection reagents are really helpful 
with protocols. 



From:   Elaine allison Hoffman via Histonet 

To: Histonet List 
Date:   11/05/2015 08:08 AM
Subject:[Histonet] IHC hand staining



Greetings Histonetters,
I need some information on "hand" immuno-staining.  We are a small GI lab 
doing Giemsa stains on H. Pylori right now, but the Lab Director wants me 
to look into IHC hand staining for H.Pylori.  Anybody out there doing hand 
staining and willing to give me a protocol to try? Or maybe point me in 
the right direction to who could help me with that.  Any reliable IHC 
staining kits recommended?  Thanks in advance for the help
Elaine Hoffman
The Gastroenterology Clinic & Endoscopy Center, IncGI Pathology 
Laboratory1622 E Market StreetWarren, Ohio   44483

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Re: [Histonet] Pas Digestion

[Jennifer MacDonald via Histonet]

The optimal temperature for most enzymes in 37 degrees Celsius. 




From:   "Lager, Loree via Histonet" 
To: "'histonet@lists.utsouthwestern.edu'" 

Date:   11/03/2015 12:38 PM
Subject:[Histonet] Pas Digestion



Hello,

This is my first time using the histonet, as our veteran user retired.

We've been having an intermittent problem with incomplete glycogen 
digestion on PASD.  Our digestion solution is 0.5%  α-Amylase, Type VI-B 
from porcine pancreas (Sigma), which we freeze in small aliquots and thaw 
for 45 minutes at room temperature before use. We digest for 20 minutes @ 
room temperature, dropping amylase on slides.

In troubleshooting the issue, we discovered amylase was several years old, 
so new amylase ordered.   The new lot (while the same product number) 
produced even worse results.  After a closer look we saw that the new lot 
contained ½ of the units/mg solid, 26 to 13, not sure why?

Anyone willing to share their procedure or suggestions to help us resolve 
this issue?

Thank you,
Loree Lager
Seattle Children's Hospital
Histology




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