We switched form malt diastase to alpha amylase and have seen a significant improvement in digestion, but as others have said enzyme activity will be destroyed at 60 degrees C. We put ours into a 37 degree C water bath. Enzymes work optimally at 37C, body temperature. As the temperature rises above 37C you will see a decrease in enzyme activity and then none at all. Jennifer
From: Joanne Clark via Histonet <histonet@lists.utsouthwestern.edu> To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Date: 05/04/2016 01:04 PM Subject: [Histonet] PAS Stain Hi Histonetters, I'm hoping someone can help me troubleshoot our PAS diastase method. We have been digesting the tissue in 0.5% diastase of malt in a 60 degree oven for 30 minutes, but do not see the glycogen being digested out. I have tried alpha amylase and beta amylase also without any luck. Does anyone have any suggestions to get the digestion to work Joanne Clark, BAAS, HT(ASCP)CM Director of Histology P. (575) 622-5600 C. (575) 317-6403 F. (575) 622-3720 TF. (800) 753-7284 pcnm.com Disclaimer: This electronic message may contain information that is proprietary, confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet