Re: [Histonet] Aqueous Mounting Media
Hello, I’ve been used many different AMM's (from many companies). In my experience lab-made AMM is the best, there is *modified method *in ihcworld.com. I’m using this method; slides are good even one year past. And no bubbles. On Wed, Nov 4, 2015 at 4:08 PM, Keyser Gerald T via Histonet < histonet@lists.utsouthwestern.edu> wrote: > Currently my lab is using Aqua-mount (Lerner Laboratories). I'm looking at > replacing it with a different Aqueous mounting media. The only stain we use > it for is Oil Red O. > > I've tried many different variations on its use and the results have been > the same. > > 1) The slide looks lovely the day that the coverslip is mounted and > then dozens of bubbles form after. Sealing does not make a difference. > Following company recommended procedure doesn't make a difference. Getting > creative doesn't make a difference. Nothing seems to make a difference. > > 2) We use a non-aqueous media surrounding the aqueous and > post-coverslip the slide. This option is messy and cannot be > re-coverslipped (for obvious reasons) if there is an air bubble. I'm sure > that this wouldn't work at all with a more delicate stain. > > Ok. I give up. Can anyone make a recommendation for a different aqueous > mounting media? > > Gerry > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Asst. Prof. Dr. Mehmet Fatih BOZKURT Department of Pathology Faculty of Veterinary Medicine Afyon Kocatepe University 03100, ANS Campus Afyonkarahisar-TURKEY Tel: +902722281312-16173/16237 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] NFkB
Hello, Anti-NFkB p65, SC-109, Santa Cruz (1/50 dilution and HieR pH 6.0) is good working on rat tissues. On Wed, Sep 2, 2015 at 4:57 PM, Hughes, Anna [JRDUS] via Histonet < histonet@lists.utsouthwestern.edu> wrote: > Hi HistoPeeps! > Anyone out there have any IHC experience with NFkB on mouse tissue? Any > antibody suggestions, good literature references, or protocols would be > welcome. > > Thanks in advance for your help! > Anna Hughes, HTL, QIHC > ahugh...@its.jnj.com > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Asst. Prof. Dr. Mehmet Fatih BOZKURT Department of Pathology Faculty of Veterinary Medicine Afyon Kocatepe University 03100, ANS Campus Afyonkarahisar-TURKEY Tel: +902722281312-16173/16237 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] CD68 and CD204 for dogs/pigs
Hello Jan, I will stain ED1 (cd68) for you on dog tissue Friday. I hope it works. On Wed, Feb 25, 2015 at 7:42 PM, Jan Shivers wrote: > Hi all, > > I do have MAC387 and it works great in dog and pig tissue. The PI is > asking for additional CD markers to macrophages (CD68 and 204). I need > them to work on dogs and pigs, but of course, there's not many vendors who > list species cross-reactivities on their data sheets beyond the usual human > and mouse. > > In my earlier trials years ago with CD68 (clone KP1), I didn't have success > using it on dog tissue. Am looking for a different clone that will > cross-react with dogs. > > Jan > > On Wed, Feb 25, 2015 at 11:08 AM, Elizabeth Chlipala > wrote: > > > Jan > > > > MAC387 is a marker that we have used successfully in canine and porcine, > > not entirely specific to macs but may work for your situation. > > > > LIz > > > > Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC > > Premier Laboratory, LLC > > PO Box 18592 > > Boulder, CO 80308 > > (303) 682-3949 office > > (303) 682-9060 fax > > (303) 881-0763 cell > > l...@premierlab.com > > www.premierlab.com > > > > March 10, 2014 is Histotechnology Professionals Day > > > > Ship to Address: > > > > Premier Laboratory, LLC > > 1567 Skyway Drive, Unit E > > Longmont, CO 80504 > > > > -Original Message- > > From: histonet-boun...@lists.utsouthwestern.edu [mailto: > > histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jan Shivers > > Sent: Tuesday, February 24, 2015 3:59 PM > > To: histonet > > Subject: [Histonet] CD68 and CD204 for dogs/pigs > > > > Can anyone recommend vendors for CD68 and CD204 that work on dogs and > pigs > > (on FFPE tissue)? I don't seem to find much information on species > > cross-reactivity online. > > > > Thanks much in advance. > > > > -- > > Jan Shivers > > Senior Scientist > > IHC/Histology Section Head > > Pathology Teaching Program > > Veterinary Diagnostic Laboratory > > University of Minnesota > > 1333 Gortner Ave. > > St. Paul, MN 55108 > > 612-624-7297 > > shive...@umn.edu > > ___ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > -- > Jan Shivers > Senior Scientist > IHC/Histology Section Head > Pathology Teaching Program > Veterinary Diagnostic Laboratory > University of Minnesota > 1333 Gortner Ave. > St. Paul, MN 55108 > 612-624-7297 > shive...@umn.edu > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Asst. Prof. Dr. Mehmet Fatih BOZKURT Department of Pathology Faculty of Veterinary Medicine Afyon Kocatepe University 03100, ANS Campus Afyonkarahisar-TURKEY Tel: +902722281312-16173/16237 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] CD31 in rabbit tissue
Hi, Santacruz Goat sc-1506 works well on rabbit tissues. Good lucks. On Tue, Oct 14, 2014 at 3:46 PM, Mandy Bell wrote: > Hi, > > We are trying to run CD31 in rabbit tissue. We've tried a couple of > different antibodies with no luck. Hoping someone can point me in the > right direction- does anyone have a CD31 they have had success with in > rabbit tissue? > > Thanks, > > Mandy Bell, HTL(ASCP) > > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Asst. Prof. Dr. Mehmet Fatih BOZKURT Department of Pathology Faculty of Veterinary Medicine Afyon Kocatepe University 03030, ANS Campus Afyonkarahisar-TURKEY Tel: +902722281312-16173/16237 Asst. Prof. Dr. Mehmet Fatih BOZKURT Manager of Veterinary Diagnostic Laboratory Afyon Kocatepe University 03030, ANS Campus Afyonkarahisar-TURKEY Tel: +902722281312-16300-16301 vetana...@aku.edu.tr ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Biossusa antibodies?
Hello, I haven't use their antibodies yet. They take 20 %fee for returned unopened antibodies. On Mon, Mar 17, 2014 at 5:48 PM, Johnson, Carole wrote: > Hello all, > > Has anyone had experience with antibodies from Biossusa? I was looking for > antibodies and came across this company but I am not familiar with them. > Any help is appreciated. > > Happy Monday, > Carole > > Carole Johnson, HT(ASCP)cm > Histopathology Section Supervisor > New Mexico Department of Agriculture > Veterinary Diagnostic Services > > > > > Confidentiality Notice: New Mexico has a very broad public records law. > Most written communications to or from state employees are public records. > Your e-mail communications may therefore be subject to public disclosure. > This e-mail, including all attachments is for the sole use of the intended > recipients. Any unauthorized review, use, disclosure or distribution is > prohibited unless specifically provided under the New Mexico Inspection of > Public Records Act. > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Asst. Prof. Dr. Mehmet Fatih BOZKURT Department of Pathology Faculty of Veterinary Medicine Afyon Kocatepe University 03030, ANS Campus Afyonkarahisar-TURKEY Tel: +902722281312-16173/16237 Asst. Prof. Dr. Mehmet Fatih BOZKURT Manager of Veterinary Diagnostic Laboratory Afyon Kocatepe University 03030, ANS Campus Afyonkarahisar-TURKEY Tel: +902722281312-16300-16301 vetana...@aku.edu.tr ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Equipment recommendations, please
Leica for Microtomy*, thermo histostar for embedding 26 Şub 2014 18:53 tarihinde "Mehmet Fatih BOZKURT" yazdı: > Leica for Microsoft, histostar for embedding. > 26 Şub 2014 17:58 tarihinde yazdı: > >> >> Which microtomes and embedding centers are you using out there? I am >> looking at the Leica RM2265 (since we're a research lab) and the Thermo >> Scientific Finesse (We have one and I like it!), but I am also open to >> suggestions. >> >> Thank you so much! >> Kathleen >> >> Principal Lab Technician >> Neurotoxicology Labs >> Molecular Pathology Facility Core >> Dept of Pharmacology & Toxicology >> Rutgers, the State University of NJ >> 41 B Gordon Road >> Piscataway, NJ 08854 >> (848) 445-1443 >> FAX (732) 445-6905 >> >> ___ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Proteinase K EIER Solutions
There is good protocol on ihcworld website. I had prepared five years ago. I'm still using it. 24 Şub 2014 17:18 tarihinde "Jennifer Leigh" yazdı: > > All- > > I am interested in Proteinase K EIER applications and was curiouis if ready-made solutions are preferred or preparation from powder and if so, how do you prepare and store the solutions? I have used the DakyCytomation ready-made Proteinase K in the past (years ago) but I am not sure if this is the most cost-effective way. Thank you so much for any ideas! > > Jennifer Oskins > > Jennifer L. Oskins > "Until one has loved an animal, part of their soul remains unawakened." > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Dried out liver bx's
Hello, You can try less alcohol fixation and before cutting you can put blocks in wet-ice box. On Wed, Feb 19, 2014 at 9:46 PM, SARAH GIBSON wrote: > We are having problems with some of our small liver bx looking burned and > dry. Does anyone have any suggestions on ways to fix this problem and any > techniques while sectionly to get a better quality section. Thanks for any > help. > > Sent from my iPad > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Asst. Prof. Dr. Mehmet Fatih BOZKURT Department of Pathology Faculty of Veterinary Medicine Afyon Kocatepe University 03030, ANS Campus Afyonkarahisar-TURKEY Tel: +902722281312-16173/16237 Asst. Prof. Dr. Mehmet Fatih BOZKURT Manager of Veterinary Diagnostic Laboratory Afyon Kocatepe University 03030, ANS Campus Afyonkarahisar-TURKEY Tel: +902722281312-16300-16301 vetana...@aku.edu.tr ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] aqueous mounting medium
I'm using modified protocol. I'm storing it at 4C. *Aqueous Mounting Medium (glycerol gelatin)* *Kisser's**Kaiser's**Glycerol jelly**Modified*Gelatine10 g40 g65 g5 gDistilled water35 ml210 ml300 ml50 mlGlycerol (glycerin)30 ml250 ml100 ml50 mlPhenol (carbolic acid) 5 ml5 ml Mix gelatin and distilled water. Heat in 50 C oven until gelatin is dissolved (it takes about 30 minutes). Add glycerin and adjust pH to 7.0 using 1N NaOH (to avoid fading of hematoxylin). Store at room temperature. Warm to 37-40 C before mounting. Note: Modified protocol works well. from ihcworld.com On Wed, Feb 19, 2014 at 8:22 PM, Caroline Miller wrote: > I use prolong gold from life tech (formerly molecular probes and now part > of the world dominating thermofisher) without Dapi as I do that separately, > for IF and aquaslip from American master tech for non IF aqueous mounting > > Good luck! > Caroline > > Caroline Miller > Gladstone Institutes > www.gladstoneinstitutes.org > > Tel: 415 7342566 > Cell: 415 2187297 > > > > > > On Feb 19, 2014, at 10:11 AM, Hans B Snyder > wrote: > > > > Hello, > > > > I have one for ORO but not for IF. If someone has one for IF, I would > be interested also. > > > > Thank you > > > > Histologistics > > Hans B Snyder > > 508.308.7800 > > h...@histologistics.com > > > >> On Feb 19, 2014, at 12:27, Jennifer MacDonald > wrote: > >> > >> Does anyone have a good "recipe" for aqueous mounting medium? > >> Thanks, > >> Jennifer > >> ___ > >> Histonet mailing list > >> Histonet@lists.utsouthwestern.edu > >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ___ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Asst. Prof. Dr. Mehmet Fatih BOZKURT Department of Pathology Faculty of Veterinary Medicine Afyon Kocatepe University 03030, ANS Campus Afyonkarahisar-TURKEY Tel: +902722281312-16173/16237 Asst. Prof. Dr. Mehmet Fatih BOZKURT Manager of Veterinary Diagnostic Laboratory Afyon Kocatepe University 03030, ANS Campus Afyonkarahisar-TURKEY Tel: +902722281312-16300-16301 vetana...@aku.edu.tr ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] rabbit cea antibody for mouse
Hello, I'm looking for rabbit CEA antibody for using on mouse tissue. Anyone can help me? Thanks. -- Mehmet Fatih BOZKURT, DVM, PhD Afyon Kocatepe University Faculty of Veterinary Medicine Department of Pathology 03030, ANS Campus Afyonkarahisar-TURKEY Tel: +902722281312-16173/16237 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] treponema spp
Hello, Do you have any protocol suggestions for *treponema spp * staining with silver and also suggestion for primary antibody doing ihc. Thanks.. -- Mehmet Fatih BOZKURT, DVM, PhD Afyon Kocatepe University Faculty of Veterinary Medicine Department of Pathology 03030, ANS Campus Afyonkarahisar-TURKEY Tel: +902722281312-16173/16237 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: [IHCRG] macrophage eq markers?
In which animals works also this antibody ? Thanks On Mon, Dec 2, 2013 at 11:20 PM, Jan Shivers wrote: > Dako's MAC 387 (cat. #: M0747) > > > On Mon, Dec 2, 2013 at 2:09 PM, Jackie Ferracone > wrote: > >> Hi All, >> >> Looking for good macrophage markers for equine tissue, FFPE IHC .? >> >> Thanks >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> Jackie Ferracone, B.Sc. >> >> Research Specialist >> >> Dept. of Pathobiology >> >> University of Pennsylvania >> >> School of Veterinary Medicine >> >> 382 West Street Road >> >> Kennett Square, PA 19348-1692 >> >> 610-925-6391 >> >> 610-350-8860 (cell) >> >> 610-925-6810 (fax) >> >> >> >> -- >> -- >> You received this message because you are subscribed to the Google >> Groups "ihcrg" group. The IHC Resource Group is a standing committee >> within the National Society for Histotechnology. >> >> To post to this group, send email to ih...@googlegroups.com >> To unsubscribe from this group, send email to >> ihcrg+unsubscr...@googlegroups.com >> For more options, visit this group at >> http://groups.google.com/group/ihcrg?hl=en >> >> To contact the National Society for Histotechnology, email: histo@nsh.orgor >> call >> 443.535.4060. >> >> --- >> You received this message because you are subscribed to the Google Groups >> "ihcrg" group. >> To unsubscribe from this group and stop receiving emails from it, send an >> email to ihcrg+unsubscr...@googlegroups.com. >> For more options, visit https://groups.google.com/groups/opt_out. >> > > > > -- > Jan Shivers > Senior Scientist > IHC/Histology Section Head > Pathology Teaching Program > Veterinary Diagnostic Laboratory > University of Minnesota > 1333 Gortner Ave. > St. Paul, MN 55108 > 612-624-7297 > shive...@umn.edu > > -- > -- > You received this message because you are subscribed to the Google > Groups "ihcrg" group. The IHC Resource Group is a standing committee > within the National Society for Histotechnology. > > To post to this group, send email to ih...@googlegroups.com > To unsubscribe from this group, send email to > ihcrg+unsubscr...@googlegroups.com > For more options, visit this group at > http://groups.google.com/group/ihcrg?hl=en > > To contact the National Society for Histotechnology, email: histo@nsh.orgor > call > 443.535.4060. > > --- > You received this message because you are subscribed to the Google Groups > "ihcrg" group. > To unsubscribe from this group and stop receiving emails from it, send an > email to ihcrg+unsubscr...@googlegroups.com. > For more options, visit https://groups.google.com/groups/opt_out. > -- Mehmet Fatih BOZKURT, DVM, PhD Afyon Kocatepe University Faculty of Veterinary Medicine Department of Pathology 03030, ANS Campus Afyonkarahisar-TURKEY Tel: +902722281312-16173/16237 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] ZInc formalin
Santa Cruz, SC-1506 is a good goat polyclonal antibody for mouse tissues. also there is rabbit IgG that is coded SC-1506R. On Thu, Sep 19, 2013 at 4:32 AM, Mesruh Turkekul wrote: > Hi Liz, > > I checked the web site and the images of FFPE sections stained with CD31 > from Dianova. It seems that it mostly stains large blood vessels. Which I > have experienced with most CD31 Ab on the market. Does this ab stain all > the capillaries in your experiments? > > Thanks again, > Mesruh > > Sent from my iPad > > On Sep 18, 2013, at 7:51 PM, Elizabeth Chlipala > wrote: > > > Dianova has an excellent antibody for CD31 in mice for FFPE tissue > samples. > > > > Liz > > > > Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC > > Premier Laboratory, LLC > > PO Box 18592 > > Boulder, CO 80308 > > (303) 682-3949 office > > (303) 881-0763 cell > > (303) 682-9060 fax > > l...@premierlab.com > > > > Ship to address: > > > > Premier Laboratory, LLC > > 1567 Skyway Drive, Unit E > > Longmont, CO 80504 > > > > From: histonet-boun...@lists.utsouthwestern.edu [ > histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mesru T [ > turke...@gmail.com] > > Sent: Wednesday, September 18, 2013 4:29 PM > > To: histonet@lists.utsouthwestern.edu > > Subject: [Histonet] ZInc formalin > > > > Dear Histonetters, > > > > I do not think there is a good CD31 (PECAM-1) antibody that will work on > > FFPE mouse tissues. If you have one please let me know. We use CD34 rat > > mono (Abcam) and Meca-32 Rat mono (Dev Hybridoma Bank Iowa Univ.) to > label > > endothelial cells of blood vessels in FFPE mouse tissues. > > There is a CD31 Rat mono from BD that will work of 10% NBF fixed, sucrose > > protected cryo embedded mouse tissues with antigen retrieval. > > The same CD31 Rat mono from BD will also work on Zinc Fixative from BD > > cat# 51-7538KZ ( it is a solution of ZnCl2 and maybe other zinc salts but > > no aldehyde) fixed paraffin embedded mouse tissues. The morphology of the > > section will be suboptimal due to this novel fixative. > > If anybody has any experince with this Zinc Fixative please would you > share? > > > > Regards, > > > > Mesruh Turkekul > > mskcc.org > > _______ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Mehmet Fatih BOZKURT, DVM, PhD Afyon Kocatepe University Faculty of Veterinary Medicine Department of Pathology 03030, ANS Campus Afyonkarahisar-TURKEY Tel: +902722281312-16173/16237 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Re: edge effect IHC
Sometimes edges of tissues can get more stain due to disruption of edge's cells more than center during the antigen retrieval. But this is not false positivity. It must pay attention to this situation On Wed, Sep 11, 2013 at 7:50 PM, Teri Johnson wrote: > Ditto to what Tim said (as usual!) What is often referred to as "edge > artifact" is sometimes excellent staining due to the edges fixed early and > well, and the interior of the tissue poorly penetrated and not as well > fixed. You can usually tell because the staining pattern is correct and > well demarcated, and easily distinguishable from the type seen in drying > artifact. > > Teri Johnson > Manager, Histology > Genomics Institute for > Novartis Research > Foundation > San Diego, CA > 858-332-4752 > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Mehmet Fatih BOZKURT, DVM, PhD Afyon Kocatepe University Faculty of Veterinary Medicine Department of Pathology 03030, ANS Campus Afyonkarahisar-TURKEY Tel: +902722281312-16173/16237 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] keeping acids in the lab.
Hello Histonet, How are you keeping daily used acids like hydrochloric, nitric, acetic and formic? Thank you.. -- Mehmet Fatih BOZKURT, DVM, PhD Afyon Kocatepe University Faculty of Veterinary Medicine Department of Pathology 03030, ANS Campus Afyonkarahisar-TURKEY Tel: +902722281312-173/237 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] help ! paraffin section
In addition to Rene's comment,to cut coagulated tissue (skin that have new wound crust) and calcified tissue is difficult. On Wed, Aug 8, 2012 at 6:45 AM, Megha Kumar wrote: > Hi All > I am trying to section adult mouse intestine and skin using paraffin > embedding. However, when i section, the tissue is torn although the rest of > the paraffin looks perfect. Please suggest why this is happening. Also, > sometimes the skin sections fall off the slides when I perform in situ > hybridization. Any ideas how to prevent this? > Please help! i am a beginner in histology and dont' know what to do! > regards > Megha > > > * > * > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Mehmet Fatih BOZKURT, DVM, PhD Afyon Kocatepe University Faculty of Veterinary Medicine Department of Pathology 03030, ANS Campus Afyonkarahisar-TURKEY Tel: +902722281312-173/237 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] PCNA sc-7907?
dilute antibody with %1 BSA.. and use %1 BSA for serum blocking... On Wed, Jul 4, 2012 at 10:59 PM, Mehmet Fatih BOZKURT wrote: > dilute antibody with %1 BSA.. and use %1 BSA for serum blocking... > > On Wed, Jul 4, 2012 at 10:21 PM, E Wolfe wrote: > >> Hello Histonet - >> >> Has anyone had any success staining with PCNA sc-7907 on frozen, >> paraformaldehyde-fixed tissue? I am experiencing a lot of background, and >> so far diluting down to 1:5000 hasn't helped at all. Any advice would be >> very much appreciated! >> ___ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > > > -- > Mehmet Fatih BOZKURT, DVM, PhD > Afyon Kocatepe University > Faculty of Veterinary Medicine > Department of Pathology > 03030, ANS Campus > Afyonkarahisar-TURKEY > Tel: +902722281312-173/237 > > -- Mehmet Fatih BOZKURT, DVM, PhD Afyon Kocatepe University Faculty of Veterinary Medicine Department of Pathology 03030, ANS Campus Afyonkarahisar-TURKEY Tel: +902722281312-173/237 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Looking for protzoal antibodies
Hello, there is antibody of neospora caninum in VMRD http://www.vmrd.com/ .. I didnt use but i have heard that is good working. for sarcocyst I can send blocks by regular mail. But Imho no need IHC for sarcocyst. On Wed, Apr 11, 2012 at 11:10 PM, Cynthia Hutchinson wrote: > Hello All, > > Does anyone know of a source for clean Neospora caninum and/or Sarcocystis > antibodies? > Also, does anyone have any positive control tissue for toxo, neo, and/or > sarco? > > Thank you, > Cindy > > Cynthia Tily Hutchinson > Rsch Asst IV > Pathobiology > Coll of Vet Med > Auburn University > Ph: 334-844-7020 > Fax: 334-844-2652 > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Mehmet Fatih BOZKURT, DVM, PhD Afyon Kocatepe University Faculty of Veterinary Medicine Department of Pathology 03030, ANS Campus Afyonkarahisar-TURKEY Tel: +902722281312-109 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Microwave use in histo lab
Hello, http://www.ebsciences.com/papers/microwave.htm I hope this helps.. On Fri, Apr 6, 2012 at 3:05 AM, Hart, Heather wrote: > Hello everyone! > > I am currently taking a class titled Current Trends & Applications in > Applied Science & Technology which is essentially a capstone course for my > BSAST degree completion. This course requires a four part research paper > on any technology of choice in the (students) related field. The topic I > have chosen is Microwave Use in the Histology Lab. I am trying to gather > information for the third module in which I need to address the topics > listed below. I would appreciate any personal input or opinions about the > topic per guidelines listed. > > Thank you for your help! > > Heather Hart, MLT (ASCP) > > > Various Perspectives and Opinions > Provide alternative perspectives from experts on technology > Political implications and influences > Public opinion on technology such as the media, consumers and > community > Your assessment of how effective the initial planning and risk > assessment was to the implementation and usage of the technology > > “You are encouraged to access other users and/or associated > technologists in order to gain insight into various perspectives and fully > understand the applications of the technology selected.” > > This message (including any attachments) may contain confidential, > proprietary, privileged and/or private information. The information is > intended to be for the use of the individual or entity designated above. > If you are not the intended recipient of this message, please notify the > sender immediately, and delete the message and any attachments. Any > disclosure, reproduction, distribution or other use of this message or any > attachments by an individual or entity other than the intended recipient is > prohibited. > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Mehmet Fatih BOZKURT, DVM, PhD Afyon Kocatepe University Faculty of Veterinary Medicine Department of Pathology 03030, ANS Campus Afyonkarahisar-TURKEY Tel: +902722281312-109 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] IHC for decalcified bone protocol
cool !! On Mon, Mar 12, 2012 at 8:49 PM, Jeffery Howery wrote: > Anyone have a Protocol for Decalcified bone for IHC? > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Mehmet Fatih BOZKURT, DVM, PhD Afyon Kocatepe University Faculty of Veterinary Medicine Department of Pathology 03030, ANS Campus Afyonkarahisar-TURKEY Tel: +902722281312-109 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] false positive CD31?
Hello, if you send images, I can say something. And I also can send cd31 positive images from different vascular tumors. On Sun, Mar 11, 2012 at 3:49 PM, Gudrun Lang wrote: > Dear histonet! > > > > Has anyone seen granular, fine dot-like, cytoplasmic CD-31 staining in > tumorcells, that was thought to be false positive? > > It looks like really specific staining, but my pathologist sent this case > to > an expert, who couldn't reproduce this staining. > > The expert is really an expert and his diagnosis is not to be bothered. > > > > We stain with dako-ab on benchmark Ultra (CC1 mild, 32min plus amplifier, > 1:50). > > > > thank you > > Gudrun Lang > > > > Biomed. Analytikerin > > histolab, Linz, Austria > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Mehmet Fatih BOZKURT, DVM, PhD Afyon Kocatepe University Faculty of Veterinary Medicine Department of Pathology 03030, ANS Campus Afyonkarahisar-TURKEY Tel: +902722281312-109 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] about some antiboides..
Hello Histonet, I’m looking for “mmp-1, mmp-2, ER beta, TIMP-1, Collagen 1, and Collagen 3” antibodies that work on canine tissue (dog). Does anybody have any experiences about these antibodies? Thanks in advance for replies. Best Wishes. -- Mehmet Fatih BOZKURT, DVM, PhD Afyon Kocatepe University Faculty of Veterinary Medicine Department of Pathology 03030, ANS Campus Afyonkarahisar-TURKEY Tel: +902722281312-109 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Re: Weigert's Iron Hematoxylin
Hi Kimberly, Imho because of the color, Weigert's Iron Hematoxylin is dark brown. Gill's 3 is blue. anilin blue is also blue. imho it would be better stain with Weigert's Iron Hematoxylin. On Wed, Mar 30, 2011 at 10:55 PM, Blundon, Kimberly < kimberly.blun...@drdc-rddc.gc.ca> wrote: > Hi Histonetters, > > > > I have a quick question regarding Weigert's Iron Hematoxylin and Gill's > 3 Hematoxylin. One of the guys here thinks that we can interchange these > 2 stains. I am attempting to perform a Masson Trichrome stain and it > calls for Weigert's Iron Hematoxylin. This person in my lab says to just > use Gill's Hematoxylin in place of the Weigert's stain. Is this right? > > > > Thanks for your help > > > > Kimberly > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] about decalcification
Hello Histonet, Does anyone know procedure of formic acid with sodium citrate decalcification method. ? i need to all of steps of procudure; fixation, decalcification and tissue processing. I will decalcificate rabbit extremites. Thanks in advance. -- Mehmet Fatih BOZKURT, DVM, PhD Afyon Kocatepe University Faculty of Veterinary Medicine Department of Pathology 03030, ANS Campus Afyonkarahisar-TURKEY Tel: +902722281312-109 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] IHC slides
You can put them %3 H2O2 for 10 minutes. and then drop primary antibody and incubate at 4 degrees overnight. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: about ki-67
Then I have two options. Firstly i have to try ThermoScientific's antibody. for alternative SC antibody. Thank you for your answers. Regards. Mehmet Fatih BOZKURT, DVM, PhD Afyon Kocatepe University Faculty of Veterinary Medicine Department of Pathology 03030, ANS Campus Afyonkarahisar-TURKEY Tel: +902722281312-109 On Mon, Feb 21, 2011 at 8:28 PM, Mehmet Fatih BOZKURT wrote: > Hello, > > This is the first mail that i sent to this group. I'm looking for Ki-67 > primary antibody for use on bovine tissue. I couldn't find on the internet > search. Does anyone know if there is a ki-67 primary antibody for available > IHC on bovine tissue ? > > Best Regards, > > Mehmet Fatih BOZKURT, DVM, PhD > Afyon Kocatepe University > Faculty of Veterinary Medicine > Department of Pathology > 03030, ANS Campus > Afyonkarahisar-TURKEY > Tel: +902722281312-109 > > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] about ki-67
Hello, This is the first mail that i sent to this group. I'm looking for Ki-67 primary antibody for use on bovine tissue. I couldn't find on the internet search. Does anyone know if there is a ki-67 primary antibody for available IHC on bovine tissue ? Best Regards, Mehmet Fatih BOZKURT, DVM, PhD Afyon Kocatepe University Faculty of Veterinary Medicine Department of Pathology 03030, ANS Campus Afyonkarahisar-TURKEY Tel: +902722281312-109 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
