[Histonet] S100
Our S100 is not working very well. I think it's too old. Can someone recommend an S100 antibody that works well in dog? It would be really nice to find a good monoclonal instead of the polyclonals. We are looking for schwannomas, astrogliomas, malignant melanomas and their metastases. Margaret Perry HT(ASCP) Veterinary Biomedical Sciences Department North Campus Drive Box 2175 South Dakota State University Brookings SD 57007 605-688-5638 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] antibody diluent
I am looking for a less expensive antibody diluent and would like some opinions on which companies are best . Are there differences between diluents when it comes to background staining? Margaret Perry HT(ASCP) Veterinary Biomedical Sciences Department North Campus Drive Box 2175 South Dakota State University Brookings SD 57007 605-688-5638 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] polymer detection
I would like to get your opinion about rabbit/mouse universal polymer detection systems. Are there any out there that have little or no background when used on canine ,feline, ovine, bovine, porcine, equine or caprine tissues? Right now I use separate polymers for rabbit or mouse antibodies. We use a DAKO autostainer. I'm just trying to get a feel about what is new to the field of polymers. Thank you for your replies. Margaret Perry HT(ASCP) Veterinary Biomedical Sciences Department North Campus Drive Box 2175 South Dakota State University Brookings SD 57007 605-688-5638 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Nile Red dye
Is there another name for Nile Red? Could it be a florescent dye? I couldn't find it on the Biological Stain commission or in a reference book from Harleco. I don't know what the stain will be used for. Margaret Perry HT(ASCP) Veterinary Biomedical Sciences Department North Campus Drive Box 2175 South Dakota State University Brookings SD 57007 605-688-5638 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] gloves
Where do you buy the Sempermed gloves? Margaret Perry HT(ASCP) Veterinary Biomedical Sciences Department Box 2175 South Dakota State University Brookings SD 57007 605-688-5638 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] what is this item called
I found something at tedpella.com. It's a glass bottom dish, catalog number 14026. There are several sizes. It looks similar to the one you posted. Margaret Perry HT(ASCP) Dept of Veterinary and Biomedical services Box 2175 South Dakota State University Brookings SD 57007 605-688-5638 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] PVK stain
We are in need of some help trouble shooting this stain. Is it normal for the tissue nuclei to stain red? If not what are we doing wrong? The Chlamydia are staining OK. Margaret Perry HT(ASCP) Dept of Veterinary and Biomedical services Box 2175 South Dakota State University Brookings SD 57007 605-688-5638 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] cytokeratin Lu5
Has anyone used this antibody on dog? If so which company did you use? Would you be willing to share your protocol? Margaret Perry HT(ASCP) Dept of Veterinary and Biomedical services Box 2175 South Dakota State University Brookings SD 57007 605-688-5638 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] picric acid
I am curious how big an explosion there would be from 1% picric acid in acetone if a little dried around the cap. Margaret Perry HT(ASCP) Dept of Veterinary and Biomedical services Box 2175 South Dakota State University Brookings SD 57007 605-688-5638 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] HE stainers
We are in the market for a new HE stainer. We probably won't be doing any special stains with it. Does anyone use the Shur/stain automated slide stainer by TBS? If so what kind of repair record does it have? Are you satisfied with it? Thanks for any info you would like to share. Margaret Perry HT(ASCP) Dept of Veterinary and Biomedical services Box 2175 South Dakota State University Brookings SD 57007 605-688-5638 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Circo antibody
We are running out of our circo antibody from Iowa and did not find it listed in their bank. Are there any recommendations for a different source? Margaret Perry HT(ASCP) Dept of Veterinary and Biomedical services Box 2175 South Dakota State University Brookings SD 57007 605-688-5638 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Need help with Laminin
I am trying to work up Laminin in dogs and cats. I have tried the antibody from Sigma L9393 with a variety of retrieval methods both enzymatic and HEIR with no success. I would like to hear from anyone who is testing for laminin. Do I need to use a different antibody? Margaret Perry HT(ASCP) Dept of Veterinary and Biomedical services Box 2175 South Dakota State University Brookings SD 57007 605-688-5638 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] nonstructured proteins
I am posting this again to see if anyone has worked with nonstructured proteins. Do they form the same crosslinks when fixed with formalin? Would antigen retrieval be of any use? Do they react with IHC the same as structured proteins? Margaret Perry HT(ASCP) Dept of Veterinary and Biomedical services Box 2175 South Dakota State University Brookings SD 57007 605-688-5638 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] looking for
Does anyone have an e-mail address for Dick Dapson, Clive Taylor, and William Grizzle? Margaret Perry HT(ASCP) Dept of Veterinary and Biomedical services Box 2175 South Dakota State University Brookings SD 57007 605-688-5638 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] unstructured proteins
Have any of you worked with the unstructured proteins? Do they form the same crosslinks when fixed with formalin? Would antigen retrieval be of any use? Margaret Perry HT(ASCP) Dept of Veterinary and Biomedical services Box 2175 South Dakota State University Brookings SD 57007 605-688-5638 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: klotz solution
We have the same problem. All we do now is make up the solution and leave out the Chloral Hydrate. I haven't talked to the pathologist about it so I don't know if it worked OK. Margaret Perry HT(ASCP) Dept of Veterinary and Biomedical services Box 2175 South Dakota State University Brookings SD 57007 605-688-5638 I've just googled and histosearched for Klotz solution but cannot find anything that doesn't require chloral hydrate. Has anyone made a Big Breakthrough in making Klotz that can be made without that pesky controlled substance? Any help will be appreciated...who knew the recipe joined the Witness Protection Program! Sally Breeden, HT(ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] laminin and collagen4 in cat and dog
We want to use laminin and Collagen IV to sort out the origin of some spindle cell tumors. Specifically, malignant peripheral nerve sheath tumors reportedly show laminin and collagen IV basement membranes vs. Fibrosarcoma which does not. We need antibodies that will work in both cat and dog. Is there anyone who has had success with these? Do you have any recommendations for these antibodies? Margaret Perry HT(ASCP) Dept of Veterinary and Biomedical services Box 2175 South Dakota State University Brookings SD 57007 605-688-5638 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] canine teeth
Hi I was given blocks today of a canine tooth that had been decaled for 8 days. We processed it on our regular overnight program. It was too hard to cut and popped out of the paraffin. The biopsy trim sheet did not indicate it was a tooth so I put the pieces in ammonium hydroxide for 10 min. It didn't help. Do you have any suggestion on how I can save this tissue? Margaret Perry HT(ASCP) Dept of Veterinary and Biomedical services Box 2175 South Dakota State University Brookings SD 57007 605-688-5638 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] microwave processors
Has anyone used the LabPulse microwave processor from EBS? We need a new microwave for staining and retrieval and thought maybe we could get one that will do processing also. I've looked at the Shurwave from Fisher, and the Mars from Hacker. Are there any other companies that have small microwave processors? Margaret Perry HT(ASCP) Dept of Veterinary and Biomedical services Box 2175 South Dakota State University Brookings SD 57007 605-688-5638 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Stupid rabbit antibodies
Have you tried the Dako Rabbit envision? We also tried the AbCam Expose Rabbit specific HRP and used Nova red from Vector or the DAB in the kit depending on which antibody we used. It is excellent , maybe even a little better than Dako's envision. I did have to use blocks such as FC receptor block Innovex, Powerblock Biogenex and serum free protein block DAKO. What I used depended on the antibody with some needing only the block provided in the kit. Good Luck. Margaret Perry HT(ASCP) Dept of Veterinary and Biomedical services Box 2175 South Dakota State University Brookings SD 57007 605-688-5638 -- Message: 17 Date: Tue, 22 Mar 2011 16:04:16 -0500 From: sgoe...@mirnarx.com Subject: [Histonet] Stupid Rabbit primaries! To: histonet@lists.utsouthwestern.edu Message-ID: d957f2a7d21959488c492a2680f9920a1c5...@svrexch.asuragen.us Content-Type: text/plain; charset=us-ascii So I haven't had to deal with rabbit polyclonal primaries in a long time because I remember how much the background sucks with them. Unfortunately the only available antibody is a rabbit polyclonal. Does anyone have any suggestions for how to eliminate the background? I have diluted almost to the point of the antigens not showing! Thanks guys and gals!! Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -- Message: 18 Date: Tue, 22 Mar 2011 16:13:04 -0500 From: Sebree Linda A lseb...@uwhealth.org Subject: RE: [Histonet] Stupid Rabbit primaries! To: sgoe...@mirnarx.com, histonet@lists.utsouthwestern.edu Message-ID: 8c023b4ab999614ba4791baeb26e273801002...@uwhc-mail01.uwhis.hosp.wisc.edu Content-Type: text/plain; charset=us-ascii Do you know for sure that its the Ab and not endogenous biotin, peroxidase or Phosphatase? You can block for all those. Otherwise there are a number of commercial blocking agents on the market...try Biocare for a start (800)799-9499 or better yet, the company that makes your antibody. Good luck. Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] liver endothelial stains
What antibody would you recommend using to label the liver endothelial cells? Is factor 8 our best marker? Also do you know of a special stain other than HE that would demonstrate these cells? Thank you. Margaret Perry HT(ASCP) Dept of Veterinary and Biomedical services Box 2175 South Dakota State University Brookings SD 57007 605-688-5638 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] special stains QC
Our lab would like to know how you handle the quality issue of verifying that the control material you use for special stains is what it's supposed to be. For example: how do you confirm that bile in a piece of liver used as a Halls control is actually bile? We use controls for bacteria or viruses that are confirmed by PCR, plating, or virus isolation. If possible we like to have the case confirmed by two different tests. If confirmed we test the paraffin blocks from that case by running a stain on them and the pathologist confirming the test positive or negative. Margaret Perry HT(ASCP) Dept of Veterinary and Biomedical services Box 2175 South Dakota State University Brookings SD 57007 605-688-5638 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] FW: WNV
From: Perry, Margaret Sent: Monday, September 27, 2010 11:41 AM To: 'tgo...@mt.gov' Subject: WNV We have a problem with the equine tissue as do other labs. A lot of samples can be done and only one will come up positive. We have good luck with avian tissues. We use DAKO envision. The antibody we use is 7H2 (81-002) from BioReliance. Proteinase K for 6 min, antibody for 30 min, DAKO mouse envision for 30 min , nova red 4 min. Hope this helps. Our control tissue is avian heart and the cells that stain are very positive. However there are very few positive cells in our control and you really have to search to find them. Margaret Perry HT(ASCP) Dept of Veterinary and Biomedical services Box 2175 South Dakota State University Brookings SD 57007 605-688-5638 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] drying ovens
Has anyone used the TDO66 Medite drying oven? Do you like it? Is the company good to work with? Margaret Perry HT(ASCP) Dept of Veterinary and Biomedical services Box 2175 South Dakota State University Brookings SD 57007 605-688-5638 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] coverslip removal
I am using aqueous mounting media, faramount, for the first time. The slides I coverslipped several days ago look dry. Will soaking in water remove the coverslip? Margaret Perry HT(ASCP) Dept of Veterinary and Biomedical services Box 2175 South Dakota State University Brookings SD 57007 605-688-5638 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] EXPOSE
Has anyone tried the new polyvalent polymer detection EXPOSE from AbCam? What did you think of it? Margaret SDSU ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] slim jims
Do you put the slim jims in formalin and then process them or just put them in the processor? Margaret ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] xylene substitutes
We use formula 83 for everything and do not have a problem with a film. We have found that we must use isopropal alcohol instead of reagent alcohol in the final dehydration steps. Margaret Perry SDSU ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] paraformaldayhde
In the Brown and Hopps gram stain can I substitute 37 g in 100 ml of powdered paraformaldyhyde for the 37% formalin or do I need to buffer it? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Gram stain
Now that I'm done with my rant I have a real question. We are trying to do a gram stain on fish and the safranine O is staining everything red. What other stain would you use? I usually have time to look at the books but unfortunately it's almost quiting time and the slides need to be done by noon Monday. Thanks Margaret Perry ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] GMS stain
We sometimes have problems with the stain if we use positive slides. Margaret ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] elisa's
Is there a list serve for elisa's? Margaret Perry ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] alcian blue van gieson stain
We want to try the double stain alcian blue Van gieson. Do you stain for the alcian blue first and then the Van gieson? We do both stains but I am unsure of how to put them together. Margaret Perry South Dakota State University ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] looking for mouse antibodies
Are there good mouse antibodies that will work on dog and cat for the following: Factor 8 GFAP Mycobacterium sp S100a ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Klotz fixative
Do you know of anyone that sells Klotz fixative? We use it in a pre vet class but would like to eliminate the choloralhydrate since it is such a hassel due to it's being a controlled substance. Is there a substitute fixative that would retain the properties of Klotz? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] need help with stain
A researcher wants to measure the length of the intestinal crypts and asked for a suggestion on what type of stain to use. I am thinking of using a phloxine/tartrazine stain and do a double stain with a GMS to show the basement membrane. What is your opinion on this? Do you have other suggestions that would work better? Hope you are warmer than here in SD! Thanks for the help. Margaret Perry South Dakota State University ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] fish scales
We are trying to cut fish scales that have been decalcified. They are chunking out when we try to cut them. I think we need to soften the keratin and I looked in the archives for the right dilution of ammonium hydroxide to use. One post said 5% the other said straight. What dilution do you recommend? I'm think it would probably be the same as what you use on toenails. Margaret Perry HT(ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Mycoplasma special stain
Hi JoAnne, Our pathologist requested we try a Faulkner stain for Mycoplasma. I found two methods. The Faulkner/Lillie and the Faulkner/Kerr variations of the Warthin Starry. One of these is a lower acid. The pathologist is looking up the reference and will call the people who wrote a paper on this. I can let you know what I find out. We would like to find a quicker method than PCR and we've tried several antibodies for IHC but they seem to cross react with other bacteria. Margaret Perry margaret.pe...@sdstate.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Warthin Faulkner method for spirochaetes
We are trying to work up this stain per pathologists special request. We usually do the Warthin-Starry method not this modification. I have the published article from Stain Technology Vol. 20 No. 3 July 1945. The protocol is a little vague and I have some questions? Step one brings the slides into acetate buffer. Step two says “impregnate sections 45 minutes at 55-60˚ C (in paraffin oven) in 1% AGNO3 in water of the same buffer level. What does water of the same buffer level mean? We interpret this as the same acetate buffer as in step one. Is this correct? Also it says to use 1 :500 acid gold chloride(HAuCl4.xH2O) to tone. Can we substitute 0.2%AuCl1? Thanks for your help. Margaret Perry South Dakota State University ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Antigen retrieval question
If we need to hold retrieved slides for awhile we hold them in water up to 4 hours. Holding in water overnight leads to no signal. We have also tried holding the slides in our Tris buffer and have found decreased or no staining. Margaret Perry ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] tris buffer
I am trying a protocol that uses 0.1M Tris/0.15M sodium chloride pH7.5, which is what I normally use. At another step in the protocol it says to wash with 0.05M Tris buffer pH 7.5. What do I use to make the 0.05M Tris buffer? Is it a combination of Tris HCl and Tris base without sodium chloride? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Tissue Embedder
We are in the market for a new embedder. What do you suggest? I tried searching the archives but only came up with older comments. Vendors please feel free to contact frank@sdstate.edumailto:frank@sdstate.edu. We may have to go with a used instrument so would like comments on good vendors. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] West Nile
Has anyone found a good antibody for west Nile FFPE that will work on horses? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Start Up Lab
I would add an eye wash station and a chemical spill kit to the list. Margaret Perry HT (ASCP) IHC Lab Manager Veterinary Science Animal Disease Research and Diagnostic Lab South Dakota State University Box 2175 North Campus Drive Brookings SD 57007 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] time in formula 83
Our processor has gone down but we can process up to the paraffin step. My question is how long can we keep the tissues in the formula 83 without any visible problems? We would like to start processing tonight and possibly infiltrate by hand tomorrow. Not optimal but it does work. Your comments are gratefully appreciated. Margaret Perry HT (ASCP) IHC Lab Manager Veterinary Science Animal Disease Research and Diagnostic Lab South Dakota State University Box 2175 North Campus Drive Brookings SD 57007 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] protease XIV
I'm trying to find a source for protease XIV other than Sigma. Any suggestions? Margaret Perry HT (ASCP) IHC Lab Manager Veterinary Science Animal Disease Research and Diagnostic Lab South Dakota State University Box 2175 North Campus Drive Brookings SD 57007 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Looking for Patsy Ruegg's e-mail address
Patsy I have a couple of questions for you but have the wrong address. Margaret Perry ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] problem eyes
Please help! We have been trying to cut whole eyes from a pig. They need to be nice enough for a publication. We are having the devil of a time because no matter what we do there are wrinkles. If we turn the waterbath up to stretch things out the retina detaches. Do any of you have suggestions? We uses Surgipath EM400 for embedding paraffin and and their infiltration medium. Is this to soft? The lowest we can turn our waterbath is 38 degrees C. Margaret Perry HT (ASCP) IHC Lab Manager Veterinary Science Animal Disease Research and Diagnostic Lab South Dakota State University Box 2175 North Campus Drive Brookings SD 57007 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] brain sections falling off slides during IHC
A few years ago we also had this problem. The brains were terrible. We found that changing our processing chemicals, especially the wax, more frequently got rid of most of our problems. We use Fisher plus slides. There is a difference in plus slides. I recommend you contact several distributors and get samples and pick the one that works best for you. After cutting we have a fan that air dries the slides until the sections look dry about 10- 15 min. The slides are dried overnight on top of our embedder so that they are heated slightly. The next day they are dried for 1 hour at 60° C. We developed this method for cattle ear notches and about the only reason tissue comes off the slide now is because the tissue is necrotic or not fixed properly. Margaret Perry HT (ASCP) IHC Lab Manager Veterinary Science Animal Disease Research and Diagnostic Lab South Dakota State University Box 2175 North Campus Drive Brookings SD 57007 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] lids for stainer containers
We are looking for a source that sells a lid that looks like wax covered cardboard. We use them on top of the Shandon slide containers holding xylene or formula 83 while waiting to coverslip. They are about 5x7 and very thin. They have been here for years so I have no idea where they came from. Any help in finding them or a substitute will be greatly appreciated. Thanks. Margaret Perry HT (ASCP) IHC Lab Manager Veterinary Science Animal Disease Research and Diagnostic Lab South Dakota State University Box 2175 North Campus Drive Brookings SD 57007 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
